18,006 results match your criteria transcriptome time


A Transcriptome Insight During Early Fish Larval Development Followed by Starvation in Seriola rivoliana.

Mar Biotechnol (NY) 2021 Oct 13. Epub 2021 Oct 13.

Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Calle IPN 195, 23096, La Paz, B.C.S, Mexico.

We investigated a time-course larval transcriptional analysis (RNA-seq) in the longfin yellowtail Seriola rivoliana, from hatching to day four at 22 °C, without providing zooplankton as food. Larval starvation is a critical physiological stage that must be prevented to ensure survival. However, the transcriptional mechanisms to endure starvation have not been investigated in marine fish. Read More

View Article and Full-Text PDF
October 2021

Upregulated NMDAR-mediated GABAergic transmission underlies autistic-like deficits in knockout mice.

Theranostics 2021 7;11(19):9296-9310. Epub 2021 Sep 7.

Center for Genetics and Developmental Systems Biology, Department of Obstetrics & Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Mutations in serotonin pathway genes, especially the serotonergic receptor subunit gene , are associated with autism. However, the association of deficiency with autism and the underlying mechanisms remain unknown. The knockout (KO) mice were generated using transcription activator-like effector nuclease technology. Read More

View Article and Full-Text PDF
September 2021

Discovery of alternatively spliced isoforms and long non-coding RNA in full length brain transcriptomes of anadromous Hilsa shad, Tenualosa ilisha (Hamilton, 1822).

Mol Biol Rep 2021 Oct 13. Epub 2021 Oct 13.

ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, India.

Background: Full length transcriptomes, achieved through long-read sequencing, along with the isoform analysis can reveal complexities in the gene expression profiles, as well as annotate the transcriptomes of non-model organisms.

Methods And Result: Full length transcripts of brain transcriptome of Tenualosa ilisha, Hilsa shad, were generated through PacBio single molecule real-time sequencing and were characterized. A total of 8. Read More

View Article and Full-Text PDF
October 2021

Effect of Costimulatory Blockade With Abatacept After Ustekinumab Withdrawal in Patients With Moderate to Severe Plaque Psoriasis: The PAUSE Randomized Clinical Trial.

JAMA Dermatol 2021 Oct 13. Epub 2021 Oct 13.

The Rockefeller University, New York, New York.

Importance: Psoriasis relapse may involve compensatory T-cell activation pathways in the presence of CD28-CD80/CD86 blockade with abatacept.

Objective: To determine whether costimulatory signaling blockade with abatacept prevents psoriasis relapse after ustekinumab withdrawal.

Design, Setting, And Participants: Psoriasis Treatment with Abatacept and Ustekinumab: a Study of Efficacy (PAUSE), a parallel-design, double-blind, placebo-controlled randomized clinical trial, was conducted at 10 sites in the US and Canada. Read More

View Article and Full-Text PDF
October 2021

Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs.

Int J Mol Sci 2021 Oct 7;22(19). Epub 2021 Oct 7.

LBN, Laboratory of Bioengineering and Nanoscience, Univ Montpellier, 34090 Montpellier, France.

We analyzed transcriptomic data from otic sensory cells differentiated from human induced pluripotent stem cells (hiPSCs) by a previously described method to gain new insights into the early human otic neurosensory lineage. We identified genes and biological networks not previously described to occur in the human otic sensory developmental cell lineage. These analyses identified and ranked genes known to be part of the otic sensory lineage program (SIX1, EYA1, GATA3, etc. Read More

View Article and Full-Text PDF
October 2021

ZNF521 Enhances MLL-AF9-Dependent Hematopoietic Stem Cell Transformation in Acute Myeloid Leukemias by Altering the Gene Expression Landscape.

Int J Mol Sci 2021 Oct 6;22(19). Epub 2021 Oct 6.

Department of Experimental and Clinical Medicine, University Magna Græcia, 88100 Catanzaro, Italy.

Leukemias derived from the MLL-AF9 rearrangement rely on dysfunctional transcriptional networks. ZNF521, a transcription co-factor implicated in the control of hematopoiesis, has been proposed to sustain leukemic transformation in collaboration with other oncogenes. Here, we demonstrate that ZNF521 mRNA levels correlate with specific genetic aberrations: in particular, the highest expression is observed in AMLs bearing MLL rearrangements, while the lowest is detected in AMLs with FLT3-ITD, NPM1, or CEBPα double mutations. Read More

View Article and Full-Text PDF
October 2021

Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony.

Int J Mol Sci 2021 Oct 6;22(19). Epub 2021 Oct 6.

Cell Therapy Center, The University of Jordan, Amman 11942, Jordan.

The stemness in keratinocyte stem cells (KSCs) is determined by their gene expression patterns. KSCs are crucial in maintaining epidermal homeostasis and wound repair and are widely used candidates for therapeutic applications. Although several studies have reported their positive identifiers, unique biomarkers for KSCs remain elusive. Read More

View Article and Full-Text PDF
October 2021

Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts.

Int J Mol Sci 2021 Sep 29;22(19). Epub 2021 Sep 29.

Department for Vascular Medicine, University Heart and Vascular Center Hamburg, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

Platelet concentrate products are increasingly used in many medical disciplines due to their regenerative properties. As they contain a variety of chemokines, cytokines, and growth factors, they are used to support the healing of chronic or complicated wounds. To date, underlying cellular mechanisms have been insufficiently investigated. Read More

View Article and Full-Text PDF
September 2021

Global Survey of the Full-Length Cabbage Transcriptome ( Var. L.) Reveals Key Alternative Splicing Events Involved in Growth and Disease Response.

Int J Mol Sci 2021 Sep 28;22(19). Epub 2021 Sep 28.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, #12 Zhong Guan Cun Nandajie Street, Beijing 100081, China.

Cabbage ( L. var. L. Read More

View Article and Full-Text PDF
September 2021

Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading Strain sp. YN2 Revealed by Transcriptome-Scale Analysis.

Int J Mol Sci 2021 Sep 28;22(19). Epub 2021 Sep 28.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

Biodegradation of 1,4-dioxane (dioxane) contamination has gained much attention for decades. In our previous work, we isolated a highly efficient dioxane degrader, sp. YN2, but the underlying mechanisms of its extraordinary degradation performance remained unresolved. Read More

View Article and Full-Text PDF
September 2021

Comparative Transcriptome Analysis of Chemoreception Organs of in Response to Rice Stripe Virus Infection.

Int J Mol Sci 2021 Sep 24;22(19). Epub 2021 Sep 24.

College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225000, China.

Many vector-borne viruses possess the ability to manipulate vector behaviors to facilitate their transmission. There is evidence that the mechanism of this phenomenon has been described in part as direct manipulation through regulating vector chemosensation. Rice stripe virus (RSV) is transmitted by the small brown planthopper, (Fallen), in a persistent, circulative-propagative manner. Read More

View Article and Full-Text PDF
September 2021

Selective Extracellular Signal-Regulated Kinase 1/2 (ERK1/2) Inhibition by the SCH772984 Compound Attenuates In Vitro and In Vivo Inflammatory Responses and Prolongs Survival in Murine Sepsis Models.

Int J Mol Sci 2021 Sep 22;22(19). Epub 2021 Sep 22.

Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, 02-781 Warsaw, Poland.

Sepsis is the leading cause of death in intensive care units worldwide. Current treatments of sepsis are largely supportive and clinical trials using specific pharmacotherapy for sepsis have failed to improve outcomes. Here, we used the lipopolysaccharide (LPS)-stimulated mouse RAW264. Read More

View Article and Full-Text PDF
September 2021

MEIS2 Is an Adrenergic Core Regulatory Transcription Factor Involved in Early Initiation of TH-MYCN-Driven Neuroblastoma Formation.

Cancers (Basel) 2021 Sep 24;13(19). Epub 2021 Sep 24.

Department for Biomolecular Medicine, Ghent University, Medical Research Building (MRB1), Corneel Heymanslaan 10, B-9000 Ghent, Belgium.

Roughly half of all high-risk neuroblastoma patients present with MYCN amplification. The molecular consequences of MYCN overexpression in this aggressive pediatric tumor have been studied for decades, but thus far, our understanding of the early initiating steps of MYCN-driven tumor formation is still enigmatic. We performed a detailed transcriptome landscaping during murine TH-MYCN-driven neuroblastoma tumor formation at different time points. Read More

View Article and Full-Text PDF
September 2021

Draft genome of Semisulcospira libertina, a species of freshwater snail.

Genomics Inform 2021 Sep 30;19(3):e32. Epub 2021 Sep 30.

Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Jinju 52727, Korea.

Semisulcospira libertina, a species of freshwater snail, is widespread in East Asia. It is important as a food source. Additionally, it is a vector of clonorchiasis, paragonimiasis, metagonimiasis, and other parasites. Read More

View Article and Full-Text PDF
September 2021

TEC kinase stabilizes PLK4 to promote liver cancer metastasis.

Cancer Lett 2021 Oct 9. Epub 2021 Oct 9.

School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China; State Key Laboratory of Liver Research (The University of Hong Kong), The University of Hong Kong, Pokfulam, Hong Kong, China. Electronic address:

Aberrated PLK4 expression has been reported in different malignancies and causes centrosome amplification, aneuploidy, and genomic instability. However, the mechanism by which PLK4 is regulated in carcinogenesis remains not fully characterised. Here, we showed that PLK4 was overexpressed in human HCC and overexpression of PLK4 predicted poorer patient prognosis. Read More

View Article and Full-Text PDF
October 2021

Integrated transcriptome profiling in THP-1 macrophages infected with bunyavirus SFTSV.

Virus Res 2021 Oct 9;306:198594. Epub 2021 Oct 9.

State Key Laboratory of Virology, School of Health Sciences, Wuhan University, Wuhan, 430071, China. Electronic address:

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne bunyavirus that causes an emerging hemorrhagic fever termed SFTS with high mortality. However, knowledge of SFTSV-host interactions is largely limited. Here, we performed a global transcriptome analysis of mRNAs and lncRNAs in THP-1 macrophages infected with SFTSV for 24 and 48 h. Read More

View Article and Full-Text PDF
October 2021

Identification and Functional Analysis of Cytokine-Like Protein CLEC-47 in Caenorhabditis elegans.

mBio 2021 Oct 12:e0257921. Epub 2021 Oct 12.

Division of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA.

A variety of effector proteins contribute to host defense in Caenorhabditis elegans. However, beyond lytic enzymes and antimicrobial peptides and proteins, little is known about the exact function of these infection-related effectors. This study set out to identify pathogen-dependent cytokine-like molecules, focusing on C-type lectin domain-containing proteins (CLECs). Read More

View Article and Full-Text PDF
October 2021

Data on RNA-seq analysis of Drosophila melanogaster during ageing.

Data Brief 2021 Oct 22;38:107413. Epub 2021 Sep 22.

USM-RIKEN Interdisciplinary Collaboration for Advanced Sciences, Universiti Sains Malaysia, Gelugor, Penang 11800, Malaysia.

Ageing is defined as gradual decline of physiological, cellular and molecular state of an organism with time. The age-associated cell dysfunctions usually cause chronic diseases such as diabetes, cancers and other age-related diseases. Many of the genes and pathways involved in ageing are conserved in different species. Read More

View Article and Full-Text PDF
October 2021

Transcriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies.

Virusdisease 2021 Sep 31;32(3):559-567. Epub 2021 Jul 31.

College of Veterinary Medicine, Key Laboratory for Clinical Diagnosis and Treatment of Animal Diseases of Ministry of Agriculture, Inner Mongolia Agricultural University, No. 29, E'erduosi east street, Saihan Region, Hohhot, People's Republic of China.

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes a serious disease to the swine industry worldwide. To understand the mechanisms of HP-PRRSV infection, RNA-seq-based transcriptome analyses were performed on porcine alveolar macrophages (PAMs) infected with a HP-PRRSV strain (TJ), a less virulent strain of a classical lineage (CH-1a), and a vaccine strain TJM-F92. Gene ontology, Kyoto Encyclopedia of Genes and Genomes analyses indicate that TJM-F92 led to significant up-regulation of gene expression for proteins associated with membrane-bound organelles. Read More

View Article and Full-Text PDF
September 2021

Transcriptome profiling and differential gene expression analysis provides insights into 24-based resistance in wheat against .

3 Biotech 2021 Nov 6;11(11):455. Epub 2021 Oct 6.

Fungal Molecular Biology Laboratory, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012 India.

Leaf rust caused by is an important disease of wheat and 24 gene confers resistance to all known pathotypes of . in India. Transcripts associated with the 24 mediated resistance were identified through transcriptome sequencing and further expression analysis of differentially regulated genes was performed using qPCR technique. Read More

View Article and Full-Text PDF
November 2021

Weighted gene co-expression network analysis unveils gene networks regulating folate biosynthesis in maize endosperm.

3 Biotech 2021 Oct 21;11(10):441. Epub 2021 Sep 21.

Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Supervision and Test Center for Ecological Environment Safety of Crops of MOA, Shanghai, China.

Folates are essential elements for human growth and development, and their deficiency can lead to serious disorders. Waxy maize is a rich source of folates; however, the regulatory mechanism underlying folate biosynthesis in the endosperm remains unclear. Here, we examined changes in the folate content of maize endosperm collected at 15, 18, 21, 24, and 27 days after pollination (DAP) using liquid chromatograph-mass spectrometry and identified genes related to folate biosynthesis using transcriptome sequencing data. Read More

View Article and Full-Text PDF
October 2021

Metabolic and Transcriptomic Profiling of Leaves Infected With Reveals Different Stages of Plant Defense Mechanisms.

Front Plant Sci 2021 22;12:730620. Epub 2021 Sep 22.

Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.

, the causal agent of gray mold disease, poses a major threat to commercial production, limiting its ornamental value and yield. The molecular and metabolic regulation mechanisms of 's defense response to infection have not been completely elucidated. Here, we performed transcriptomic and metabolomic analyses of resistant oriental hybrid "Sorbonne" to understand the molecular basis of gray mold disease resistance in gray mold disease. Read More

View Article and Full-Text PDF
September 2021

Transcriptome Reprogramming of Tomato Orchestrate the Hormone Signaling Network of Systemic Resistance Induced by .

Front Plant Sci 2021 23;12:721193. Epub 2021 Sep 23.

ICAR-National Institute for Plant Biotechnology, ICAR-IARI, New Delhi, India.

is a potential biological control agent effective against various plant pathogens. Several reports are available on the mycoparastism and antibiosis mechanisms of against plant pathogenic fungi, whereas a few states induced resistance. The potential induced defense component of (Cg-2) was evaluated against early blight disease of tomato ( and further, global RNA sequencing was performed to gain deep insight into its mechanism. Read More

View Article and Full-Text PDF
September 2021

A Comprehensive Prognostic and Immunological Analysis of a Six-Gene Signature Associated With Glycolysis and Immune Response in Uveal Melanoma.

Front Immunol 2021 22;12:738068. Epub 2021 Sep 22.

Reproductive Medicine Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan, China.

Uveal melanoma (UM) is a subtype of melanoma with poor prognosis. This study aimed to construct a new prognostic gene signature that can be used for survival prediction and risk stratification of UM patients. In this work, transcriptome data from the Molecular Signatures Database were used to identify the cancer hallmarks most relevant to the prognosis of UM patients. Read More

View Article and Full-Text PDF
September 2021

Transcriptomics Analysis Reveals Shared Pathways in Peripheral Blood Mononuclear Cells and Brain Tissues of Patients With Schizophrenia.

Front Psychiatry 2021 22;12:716722. Epub 2021 Sep 22.

NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Schizophrenia is a serious mental disorder with complicated biological mechanisms. Few studies explore the transcriptional features that are shared in brain tissue and peripheral blood. In the present study, we aimed to explore the biological pathways with similar expression patterns in both peripheral blood mononuclear cells (PBMCs) and brain tissues. Read More

View Article and Full-Text PDF
September 2021

[Expression and Prognostic Value of Metabolism-related Genes in Pediatric Acute Lymphoblastic Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Oct;29(5):1375-1379

Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China E-mail:

Objective: To analyze the expression and prognostic value of metabolism-related genes in pediatric acute lymphoblastic leukemia (ALL), and explore the potential prognostic biomarkers or therapeutic targets.

Methods: Transcriptome data from 84 children with B-cell ALL at the time of diagnosis and prior to any treatment were used to analyze the differential gene expression. A prognostic scoring system based on the expression of the metabolism-related genes was constructed using Cox and Lasso regression methods. Read More

View Article and Full-Text PDF
October 2021

Comparative transcriptomic analysis of races 1, 2, 5 and 6 of Fusarium oxysporum f.sp. pisi in a susceptible pea host identifies differential pathogenicity profiles.

BMC Genomics 2021 Oct 9;22(1):734. Epub 2021 Oct 9.

Australian Institute of Botanical Science, Royal Botanic Gardens & Domain Trust, Sydney, NSW, Australia.

Background: The fungal pathogen Fusarium oxysporum f.sp. pisi (Fop) causes Fusarium wilt in peas. Read More

View Article and Full-Text PDF
October 2021

From innate to adaptive immunity: Abomasal transcriptomic responses of merino sheep to Haemonchus contortus infection.

Mol Biochem Parasitol 2021 Oct 6;246:111424. Epub 2021 Oct 6.

College of Life Science, Hubei Normal University, Huangshi, Hubei, 435002, China. Electronic address:

Although many important mediators and critical pathways are found to be involved in host immune responses to Haemonchus contortus infection, the initial responses to infection in the naïve and in the previously exposed state have not been compared at the transcriptional level. To further understand the development of adaptive immunity to H. contortus infection, we compared the early abomasal gene expression patterns between a primary and a tertiary challenge for four lines of sheep to discover differentially expressed genes (DEGs). Read More

View Article and Full-Text PDF
October 2021

Testis transcriptome profiling identified lncRNAs involved in spermatogenic arrest of cattleyak.

Funct Integr Genomics 2021 Oct 9. Epub 2021 Oct 9.

Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, 610041, Sichuan, China.

Cattleyaks are the crossbred offspring between cattle and yaks, exhibiting the prominent adaptability to the harsh environment as yaks and much higher growth performances than yaks around Qinghai-Tibet plateau. Unfortunately, cattleyak cannot be effectively used in yak breeding due to its male infertility resulted from spermatogenic arrest. In this study, we performed RNA sequencing (RNA-seq) and bioinformatics analysis to determine the expression profiles of long noncoding RNA (lncRNA) from cattleyak and yak testis. Read More

View Article and Full-Text PDF
October 2021

Characterization of the early gene expression profile in Populus ussuriensis under cold stress using PacBio SMRT sequencing integrated with RNA-seq reads.

Tree Physiol 2021 Oct 9. Epub 2021 Oct 9.

State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.

Populus ussuriensis is an important and fast-growing afforestation plant species in north-eastern China. The whole-genome sequencing of P. ussuriensis has not been completed. Read More

View Article and Full-Text PDF
October 2021