308 results match your criteria transcriptome secretome

Multiomic analysis of stretched osteocytes reveals processes and signalling linked to bone regeneration and cancer.

NPJ Regen Med 2021 Jun 7;6(1):32. Epub 2021 Jun 7.

Department of Biosciences, Centre for Health, Ageing and Understanding Disease (CHAUD), School of Science and Technology, Nottingham Trent University, Nottingham, UK.

Exercise is a non-pharmacological intervention that can enhance bone regeneration and improve the management of bone conditions like osteoporosis or metastatic bone cancer. Therefore, it is gaining increasing importance in an emerging area of regenerative medicine-regenerative rehabilitation (RR). Osteocytes are mechanosensitive and secretory bone cells that orchestrate bone anabolism and hence postulated to be an attractive target of regenerative exercise interventions. Read More

View Article and Full-Text PDF

Transcriptomic Profiling of the Liver Sinusoidal Endothelium during Cirrhosis Reveals Stage-Specific Secretory Signature.

Cancers (Basel) 2021 May 29;13(11). Epub 2021 May 29.

Liver Vascular Biology Research Group, Barcelona Hepatic Hemodynamic Unit, IDIBAPS, 08036 Barcelona, Spain.

The poor prognosis of chronic liver disease (CLD) generates the need to investigate the evolving mechanisms of disease progression, thus disclosing therapeutic targets before development of clinical complications. Considering the central role of liver sinusoidal endothelial cells (LSECs) in pre-neoplastic advanced CLD, the present study aimed at investigating the progression of CLD from an endothelial holistic perspective. RNAseq defined the transcriptome of primary LSECs isolated from three pre-clinical models of advanced CLD, during the progression of the disease, and from fresh human cirrhotic tissue. Read More

View Article and Full-Text PDF

Targeting mesenchymal stromal cells plasticity to reroute acute myeloid leukemia course.

Blood 2021 05 19. Epub 2021 May 19.

Women’s and Children’s Health Department, Haematology-Oncology Clinic and Lab, University of Padua, Padua, Italy

Bone marrow (BM) microenvironment contributes to the regulation of normal hematopoiesis through a finely tuned balance of self-renewal and differentiation processes, cell-cell interaction and secretion of cytokines that during leukemogenesis are altered and favor tumor cell growth. In pediatric acute myeloid leukemia (AML), chemotherapy is the standard of care, but still >30% of patients relapse. The need to accelerate the evaluation of innovative medicines prompted us to investigate the mesenchymal stromal cells (MSCs) role in the leukemic niche to define its contribution to the mechanisms of leukemia escape. Read More

View Article and Full-Text PDF

Integrated OMICs unveil the bone-marrow microenvironment in human leukemia.

Cell Rep 2021 May;35(6):109119

Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK. Electronic address:

The bone-marrow (BM) niche is the spatial environment composed by a network of multiple stromal components regulating adult hematopoiesis. We use multi-omics and computational tools to analyze multiple BM environmental compartments and decipher their mutual interactions in the context of acute myeloid leukemia (AML) xenografts. Under homeostatic conditions, we find a considerable overlap between niche populations identified using current markers. Read More

View Article and Full-Text PDF

The response of two polar monocyte subsets to inflammation.

Biomed Pharmacother 2021 Jul 27;139:111614. Epub 2021 Apr 27.

Рeoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia; Scientific Research Institute of Human Morphology, 117418 Moscow, Russia.

Macrophages are a central component of innate immunity that play an important role in the defense of the organism. Macrophages are highly plastic and are activated by interaction with other cells and environmental factors. In this work, we study the effect of lipopolysaccharide on macrophages derived from the two most polar (CD14+ and CD16+ monocytes) as well as the intermediate subset of blood monocytes from healthy donors and assess what happens to the subset most prone to polarization on the transcriptomic and proteomic level. Read More

View Article and Full-Text PDF

Phylogeny, tissue-specific expression, and activities of root-secreted purple acid phosphatases for P uptake from ATP in P starved poplar.

Plant Sci 2021 Jun 6;307:110906. Epub 2021 Apr 6.

Forest Botany and Tree Physiology, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany; Laboratory for Radio-Isotopes, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany; Center of Sustainable Land Use, University of Göttingen, Büsgenweg 1, 37077 Göttingen, Germany; Center for Molecular Biosciences (GZMB), Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany. Electronic address:

Plants secrete purple acid phosphatases (PAPs) under phosphorus (P) shortage but the contribution of plant PAPs to P acquisition is not well understood. The goals of this study were to investigate comprehensively the transcription patterns of PAPs under P shortage in poplar (Populus × canescens), to identify secreted PAPs and to characterize their contribution to mobilize organic P. Phylogenetic analyses of the PAP family revealed 33 putative members. Read More

View Article and Full-Text PDF

Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600.

Biotechnol Biofuels 2021 Apr 17;14(1):96. Epub 2021 Apr 17.

Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.

Background: Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydrate-active enzymes and non-enzymatic Fenton mechanism. Differences in the lignocellulose catabolism among closely related brown rots are not completely understood. Read More

View Article and Full-Text PDF

Diabetic kidney disease alters the transcriptome and function of human adipose-derived mesenchymal stromal cells but maintains immunomodulatory and paracrine activities important for renal repair.

Diabetes 2021 Apr 15. Epub 2021 Apr 15.

Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, Rochester, MN, USA.

Mesenchymal stem/stromal cells (MSC) facilitate repair in experimental diabetic kidney disease (DKD). However, the hyperglycemic and uremic milieu may diminish regenerative capacity of patient-derived therapy. We hypothesized that DKD reduces human MSC paracrine function. Read More

View Article and Full-Text PDF

Eudiplozoon nipponicum (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling.

BMC Genomics 2021 Apr 15;22(1):274. Epub 2021 Apr 15.

Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic.

Background: Ectoparasites from the family Diplozoidae (Platyhelminthes, Monogenea) belong to obligate haematophagous helminths of cyprinid fish. Current knowledge of these worms is for the most part limited to their morphological, phylogenetic, and population features. Information concerning the biochemical and molecular nature of physiological processes involved in host-parasite interaction, such as evasion of the immune system and its regulation, digestion of macromolecules, suppression of blood coagulation and inflammation, and effect on host tissue and physiology, is lacking. Read More

View Article and Full-Text PDF

Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann-Like Adipose Mesenchymal Stromal Cells.

Adv Biol (Weinh) 2021 04 4;5(4):e2000271. Epub 2021 Mar 4.

Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK.

Mesenchymal stromal cells from adipose tissue (AD-MSCs) exhibit favorable clinical traits for autologous transplantation and can develop 'Schwann-like' phenotypes (sAD-MSCs) to improve peripheral nerve regeneration, where severe injuries yield insufficient recovery. However, sAD-MSCs regress without biochemical stimulation and detach from conduits under unfavorable transplant conditions, negating their paracrine effects. Graphene-derived materials support AD-MSC attachment, regulating cell adhesion and function through physiochemistry and topography. Read More

View Article and Full-Text PDF

Insights into the multitrophic interactions between the biocontrol agent Bacillus subtilis MBI 600, the pathogen Botrytis cinerea and their plant host.

Microbiol Res 2021 Jul 2;248:126752. Epub 2021 Apr 2.

Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala Biocenter, Box 7026, SE-750 07, Uppsala, Sweden. Electronic address:

Botrytis cinerea is a plant pathogen causing the gray mold disease in a plethora of host plants. The control of the disease is based mostly on chemical pesticides, which are responsible for environmental pollution, while they also pose risks for human health. Furthermore, B. Read More

View Article and Full-Text PDF

Trematode Proteomics: Recent Advances and Future Directions.

Pathogens 2021 Mar 16;10(3). Epub 2021 Mar 16.

School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, Northern Ireland, UK.

Trematodes cause disease in millions of people worldwide, but the absence of commercial vaccines has led to an over-reliance on a handful of monotherapies to control infections. Since drug-resistant fluke populations are emerging, a deeper understanding of parasite biology and host interactions is required to identify new drug targets and immunogenic vaccine candidates. Mass spectrometry-based proteomics represents a key tool to that end. Read More

View Article and Full-Text PDF

Probing the Sialomes in Potential Anti-Tick Vaccine Candidates: A Reverse Vaccinology Approach.

Biomedicines 2021 Mar 31;9(4). Epub 2021 Mar 31.

Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal.

In the wake of the 'omics' explosion of data, reverse vaccinology approaches are being applied more readily as an alternative for the discovery of candidates for next generation diagnostics and vaccines. Promising protective antigens for the control of ticks and tick-borne diseases can be discovered by mining available omics data for immunogenic epitopes. The present study aims to explore the previously obtained sialotranscriptome during both feeding and infection, to select antigenic targets that are either membrane-associated or a secreted protein, as well as unique to the ectoparasite and not present in the mammalian host. Read More

View Article and Full-Text PDF

Beyond the Extracellular Vesicles: Technical Hurdles, Achieved Goals and Current Challenges When Working on Adipose Cells.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Institute for Tumor Immunology, Center for Tumor Biology and Immunology (ZTI), Philipps University of Marburg, 35043 Marburg, Germany.

Adipose tissue and its crosstalk with other organs plays an essential role in the metabolic homeostasis of the entire body. Alteration of this communication (i.e. Read More

View Article and Full-Text PDF

Oncolytic Virus Therapy Alters the Secretome of Targeted Glioblastoma Cells.

Cancers (Basel) 2021 Mar 14;13(6). Epub 2021 Mar 14.

Harvey Cushing Neuro-Oncology Laboratories, Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Oncolytic virus (OV) therapy, which is being tested in clinical trials for glioblastoma, targets cancer cells, while triggering immune cells. Yet OV sensitivity varies from patient to patient. As OV therapy is regarded as an anti-tumor vaccine, by making OV-infected cancer cells secrete immunogenic proteins, linking these proteins to transcriptome would provide a measuring tool to predict their sensitivity. Read More

View Article and Full-Text PDF

Schwann cell plasticity regulates neuroblastic tumor cell differentiation via epidermal growth factor-like protein 8.

Nat Commun 2021 03 12;12(1):1624. Epub 2021 Mar 12.

St. Anna Children's Cancer Research Institute (CCRI), Vienna, Austria.

Adult Schwann cells (SCs) possess an inherent plastic potential. This plasticity allows SCs to acquire repair-specific functions essential for peripheral nerve regeneration. Here, we investigate whether stromal SCs in benign-behaving peripheral neuroblastic tumors adopt a similar cellular state. Read More

View Article and Full-Text PDF

Endometrium On-a-Chip Reveals Insulin- and Glucose-induced Alterations in the Transcriptome and Proteomic Secretome.

Endocrinology 2021 Jun;162(6)

Discovery and Translational Sciences Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, Leeds, UK.

The molecular interactions between the maternal environment and the developing embryo are key for early pregnancy success and are influenced by factors such as maternal metabolic status. Our understanding of the mechanism(s) through which these individual nutritional stressors alter endometrial function and the in utero environment for early pregnancy success is, however, limited. Here we report, for the first time, the use of an endometrium-on-a-chip microfluidics approach to produce a multicellular endometrium in vitro. Read More

View Article and Full-Text PDF

Reproducing diabetic retinopathy features using newly developed human induced-pluripotent stem cell-derived retinal Müller glial cells.

Glia 2021 Jul 8;69(7):1679-1693. Epub 2021 Mar 8.

Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France.

Muller glial cells (MGCs) are responsible for the homeostatic and metabolic support of the retina. Despite the importance of MGCs in retinal disorders, reliable and accessible human cell sources to be used to model MGC-associated diseases are lacking. Although primary human MGCs (pMGCs) can be purified from post-mortem retinal tissues, the donor scarcity limits their use. Read More

View Article and Full-Text PDF

Multiomics analyses of cytokines, genes, miRNA, and regulatory networks in human mesenchymal stem cells expanded in stirred microcarrier-spinner cultures.

Stem Cell Res 2021 May 26;53:102272. Epub 2021 Feb 26.

Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), Singapore. Electronic address:

Mesenchymal stem cells (MSCs) are of great clinical interest as a form of allogenic therapy due to their excellent regenerative and immunomodulatory effects for various therapeutic indications. Stirred suspension bioreactors using microcarriers (MC) have been used for large-scale production of MSCs compared to planar cultivation systems. Previously, we have demonstrated that expansion of MSCs in MC-spinner cultures improved chondrogenic, osteogenic, and cell migration potentials as compared to monolayer-static cultures. Read More

View Article and Full-Text PDF

Transcriptome Profiles of during the Early Infection of Resistant and Susceptible Maize Isogenic Lines.

J Fungi (Basel) 2021 Feb 19;7(2). Epub 2021 Feb 19.

State Key Laboratory of Plant Physiology and Biochemistry, College of Agronomy and Biotechnology, National Maize Improvement Center, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, 2 West Yuanmingyuan Road, Beijing 100193, China.

The biotrophic fungus causes destructive head smut disease in maize ( L.). To explore the pathogenicity arsenal of this fungus, we tracked its transcriptome changes during infection of the maize seedling mesocotyls of two near-isogenic lines, HZ4 and HZ4R, differing solely in the disease resistance gene . Read More

View Article and Full-Text PDF
February 2021

Plasma-Signature-Model for End-Stage Liver Disease Score to Predict Survival in Severe Alcoholic Hepatitis.

Clin Gastroenterol Hepatol 2021 Mar 2. Epub 2021 Mar 2.

Liver Tumor Translational Research Program, Simmons Comprehensive Cancer Center, Division of Digestive and Liver Diseases, Department of Internal Medicine, Dallas, Texas. Electronic address:

Background & Aims: Severe alcoholic hepatitis (AH) is a highly lethal condition and it is still a challenge to predict the outcome. We previously identified and validated a composite score of hepatic 123-gene prognostic signature and the model for end-stage liver disease (MELD) score: gene signature-MELD. However, the need for liver biopsy limits its clinical application. Read More

View Article and Full-Text PDF

Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes.

Aging (Albany NY) 2021 02 12;13(4):4747-4777. Epub 2021 Feb 12.

Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies and University of California - San Diego, La Jolla, CA 92037, USA.

Senescent cells produce chronic inflammation that contributes to the diseases and debilities of aging. How this process is orchestrated in epithelial cells, the origin of human carcinomas, is poorly understood. We used human normal oral keratinocytes (NOKs) to elucidate senescence programs in a prototype primary mucosal epithelial cell that senesces spontaneously. Read More

View Article and Full-Text PDF
February 2021

Transcriptome and secretome analysis of intra-mammalian life-stages of the emerging helminth pathogen, Calicophoron daubneyi reveals adaptation to a unique host environment.

Mol Cell Proteomics 2021 Feb 10:100055. Epub 2021 Feb 10.

School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast, Northern Ireland. Electronic address:

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock which has seen a rapid rise in prevalence throughout Western Europe in recent years. Following ingestion of metacercariae (parasite cysts) by the mammalian host, newly-excysted juveniles (NEJs) emerge and invade the duodenal submucosa which causes significant pathology in heavy infections. The immature larvae then migrate upwards, along the gastrointestinal tract, and enter the rumen where they mature and begin to produce eggs. Read More

View Article and Full-Text PDF
February 2021

Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228.

Biotechnol Biofuels 2021 Jan 25;14(1):31. Epub 2021 Jan 25.

Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.

Background: Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse, P. funiculosum has been underestimated due to a lack of reliable genetic engineering tools. Read More

View Article and Full-Text PDF
January 2021

Macrophage-Derived Inflammation Induces a Transcriptome Makeover in Mesenchymal Stromal Cells Enhancing Their Potential for Tissue Repair.

Int J Mol Sci 2021 Jan 14;22(2). Epub 2021 Jan 14.

Shirley Ryan AbilityLab, Chicago, IL 60611, USA.

Pre-clinical and clinical studies revealed that mesenchymal stromal cell (MSC) transplants elicit tissue repair. Conditioning MSC prior to transplantation may boost their ability to support repair. We investigated macrophage-derived inflammation as a means to condition MSC by comprehensively analyzing their transcriptome and secretome. Read More

View Article and Full-Text PDF
January 2021

The human G protein-coupled ATP receptor P2Y is a target for anti-inflammatory strategies.

Br J Pharmacol 2021 Apr 19;178(7):1541-1555. Epub 2021 Feb 19.

Immunotherapy Unit, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria.

Background And Purpose: The ATP receptor P2Y , which couples to G and G proteins, senses cell stress and promotes cytoprotective responses. P2Y receptors are upregulated during differentiation of M2 macrophages. However, it is unclear whether and how P2Y receptors contribute to the anti-inflammatory properties of M2 macrophages. Read More

View Article and Full-Text PDF

Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver.

BMC Genomics 2021 Jan 11;22(1):46. Epub 2021 Jan 11.

Zoology Department, School of Natural Sciences, Centre for One Health, Ryan Institute, National University of Ireland Galway, Galway, Ireland.

Background: The major pathogenesis associated with Fasciola hepatica infection results from the extensive tissue damage caused by the tunnelling and feeding activity of immature flukes during their migration, growth and development in the liver. This is compounded by the pathology caused by host innate and adaptive immune responses that struggle to simultaneously counter infection and repair tissue damage.

Results: Complementary transcriptomic and proteomic approaches defined the F. Read More

View Article and Full-Text PDF
January 2021

Transcriptome during the Infection Process of the Bryophyte and Angiosperms.

J Fungi (Basel) 2020 Dec 28;7(1). Epub 2020 Dec 28.

Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, Uruguay.

is a necrotrophic pathogen that causes grey mold in many plant species, including crops and model plants of angiosperms. also infects and colonizes the bryophyte (previously ), which perceives the pathogen and activates defense mechanisms. However, these defenses are not sufficient to stop fungal invasion, leading finally to plant decay. Read More

View Article and Full-Text PDF
December 2020

Prophage-Related Gene Contributes to Cell Membrane Integrity and Growth of CHN25.

Front Cell Infect Microbiol 2020 9;10:595709. Epub 2020 Dec 9.

Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), China Ministry of Agriculture, Shanghai, China.

is a leading seafood-borne pathogen that can cause acute gastroenteritis and even death in humans. In aquatic ecosystems, phages constantly transform bacterial communities by horizontal gene transfer. Nevertheless, biological functions of prophage-related genes in remain to be fully unveiled. Read More

View Article and Full-Text PDF

Cellular Senescence in Kidney Fibrosis: Pathologic Significance and Therapeutic Strategies.

Front Pharmacol 2020 11;11:601325. Epub 2020 Dec 11.

State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Age-related disorders such as chronic kidney disease (CKD) are increasingly prevalent globally and pose unprecedented challenges. In many aspects, CKD can be viewed as a state of accelerated and premature aging. Aging kidney and CKD share many common characteristic features with increased cellular senescence, a conserved program characterized by an irreversible cell cycle arrest with altered transcriptome and secretome. Read More

View Article and Full-Text PDF
December 2020