247 results match your criteria transcriptome grapevine


The grapevine (Vitis vinifera L.) floral transcriptome in Pinot noir variety: identification of tissue-related gene networks and whorl-specific markers in pre- and post-anthesis phases.

Hortic Res 2021 Sep 1;8(1):200. Epub 2021 Sep 1.

Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Campus of Agripolis, V. le dell'Università 16, 35020, Legnaro, Padova, Italy.

The comprehension of molecular processes underlying the development and progression of flowering in plants is a hot topic, not only because that often the products of interest for human and animal nutrition are linked to the development of fruits or seeds, but also because the processes of gametes formation occurring in sexual organs are at the basis of recombination and genetic variability which constitutes the matter on which evolution acts, whether understood as natural or human driven. In the present study, we used an NGS approach to produce a grapevine flower transcriptome snapshot in different whorls and tissues including calyx, calyptra, filament, anther, stigma, ovary, and embryo in both pre- and post-anthesis phases. Our investigation aimed at identifying hub genes that unequivocally distinguish the different tissues providing insights into the molecular mechanisms that are at the basis of floral whorls and tissue development. Read More

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September 2021

Physiological and transcriptomic analysis of Cabernet Sauvginon (Vitis vinifera L.) reveals the alleviating effect of exogenous strigolactones on the response of grapevine to drought stress.

Plant Physiol Biochem 2021 Aug 14;167:400-409. Epub 2021 Aug 14.

College of Enology, Northwest A & F University, Yangling, 712100, Shaanxi, China. Electronic address:

Drought stress can significantly affect the growth and yield of grapevine. The application of exogenous strigolactone can relieve the drought symptoms of grapevine; however, little is known about the transcription levels in grapevine under drought stress following exogenous strigolactone application. The mitigative effect of exogenous strigolactone on grapevine leaves under drought stress was studied by transcriptome analysis based on RNA sequencing. Read More

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Transcriptomic analysis of temporal shifts in berry development between two grapevine cultivars of the Pinot family reveals potential genes controlling ripening time.

BMC Plant Biol 2021 Jul 7;21(1):327. Epub 2021 Jul 7.

Genetics and Genomics of Plants, Faculty of Biology & Center for Biotechnology, Bielefeld University, Bielefeld, Germany.

Background: Grapevine cultivars of the Pinot family represent clonally propagated mutants with major phenotypic and physiological differences, such as different colour or shifted ripening time, as well as changes in important viticultural traits. Specifically, the cultivars 'Pinot Noir' (PN) and 'Pinot Noir Precoce' (PNP, early ripening) flower at the same time, but vary in the beginning of berry ripening (veraison) and, consequently, harvest time. In addition to genotype, seasonal climatic conditions (i. Read More

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Functional Characterization of VDACs in Grape and Its Putative Role in Response to Pathogen Stress.

Front Plant Sci 2021 16;12:670505. Epub 2021 Jun 16.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.

Voltage-dependent anion channels (VDACs) are the most abundant proteins in the mitochondrial outer membranes of all eukaryotic cells. They participate in mitochondrial energy metabolism, mitochondria-mediated apoptosis, and cell growth and reproduction. Here, the chromosomal localizations, gene structure, conserved domains, and phylogenetic relationships were analyzed. Read More

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Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary.

Viruses 2021 06 10;13(6). Epub 2021 Jun 10.

Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, Ménesi Road 44, H-1118 Budapest, Hungary.

Grapevine virus T (GVT) is a recently described foveavirus, which was identified from a transcriptome of a Teroldego grapevine cultivar in 2017. Recently, we surveyed vineyards and rootstock plantations in Hungary using small RNA (sRNA) high-throughput sequencing (HTS), at a time when GVT had not yet been described. A re-analysis of our sRNA HTS datasets and a survey of grapevines by RT-PCR revealed the presence of GVT in most of the vineyards tested, while at rootstock fields its presence was very rare. Read More

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Transcriptional profiling reveals multiple defense responses in downy mildew-resistant transgenic grapevine expressing a TIR-NBS-LRR gene located at the MrRUN1/MrRPV1 locus.

Hortic Res 2021 Jul 1;8(1):161. Epub 2021 Jul 1.

Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China.

Grapevine downy mildew (DM) is a destructive oomycete disease of viticulture worldwide. MrRPV1 is a typical TIR-NBS-LRR type DM disease resistance gene cloned from the wild North American grapevine species Muscadinia rotundifolia. However, the molecular basis of resistance mediated by MrRPV1 remains poorly understood. Read More

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Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Shang Qiao Zhong Lu, Chaoyang DistrictBeijing, China, 100024.

To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus (CNSV), apple stem grooving virus (ASGV), lychnis mottle virus (LycMoV), grapevine line pattern virus (GLPV), and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, Citrivirus in Betaflexiviridae), peony betaflexivirus 1 (PeV1, unclassified in Betaflexiviridae), and peony leafroll-associated virus (PLRaV, Ampelovirus in Closteroviridae). PYaCV was 8,666 nucleaotides (nt) in length, comprising three open reading frames (ORFs) and shared 63. Read More

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Transcriptional, hormonal, and metabolic changes in susceptible grape berries under powdery mildew infection.

J Exp Bot 2021 Jun 9. Epub 2021 Jun 9.

BioISI - Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, Campo Grande, Lisboa, Portugal.

Vitis vinifera berries are extremely sensitive to infection by the biotrophic pathogen Erysiphe necator causing powdery mildew disease and deleterious effects on grape and wine quality. The combined analysis of the transcriptome and metabolome associated with this common fungal infection has not been previously carried out in any fruit. In order to identify the molecular, hormonal and metabolic mechanisms associated with infection, healthy and naturally infected Carignan berries were collected at two developmental stages: late green (EL33) and early véraison (EL35). Read More

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Bacteriophage-Host Association in the Phytoplasma Insect Vector .

Pathogens 2021 May 17;10(5). Epub 2021 May 17.

Institute for Sustainable Plant Protection, National Research Council of Italy, Strada delle Cacce 73, 10135 Torino, Italy.

Insect vectors transmit viruses and bacteria that can cause severe diseases in plants and economic losses due to a decrease in crop production. Insect vectors, like all other organisms, are colonized by a community of various microorganisms, which can influence their physiology, ecology, evolution, and also their competence as vectors. The important ecological meaning of bacteriophages in various ecosystems and their role in microbial communities has emerged in the past decade. Read More

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Transcriptional and metabolite analysis reveal a shift in fruit quality in response to calcium chloride treatment on "Kyoho" grapevine.

J Food Sci Technol 2021 Jun 19;58(6):2246-2257. Epub 2020 Aug 19.

Key Laboratory of Genetics and Fruit Development, College of Horticulture, Nanjing Agricultural University, Nanjing, 210018 PR China.

'Kyoho' grapevine () treated by calcium ions solution has been proved as an effective treatment to extend grape quality during storage to reduce disease, but its molecular mechanism was not clear yet. In the current work, grape berries were treated with different concentration of Calcium chloride (CaCl) solution, and their effects on antioxidant enzyme activity and transcriptome and metabolome in fruit were investigated. CaCl treatments reduced weight loss and inhibited the decrement of flesh firmness. Read More

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Multi-omics analyses on the response mechanisms of 'Shine Muscat' grapevine to low degree of excess copper stress (Low-ECS).

Environ Pollut 2021 Oct 30;286:117278. Epub 2021 Apr 30.

Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, China; Fruit Crop Genetic Improvement and Seedling Propagation Engineering Center of Jiangsu Province, Nanjing, 210095, China.

Copper stress is one of the most severe heavy metal stresses in plants. Grapevine has a relatively higher copper tolerance than other fruit crops. However, there are no reports regarding the tolerance mechanisms of the 'Shine Muscat' ('SM') grape to a low degree of excess copper stress (Low-ECS). Read More

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October 2021

Integration of early disease-resistance phenotyping, histological characterization, and transcriptome sequencing reveals insights into downy mildew resistance in impatiens.

Hortic Res 2021 May 1;8(1):108. Epub 2021 May 1.

University of Florida, IFAS, Department of Environmental Horticulture, Gulf Coast Research and Education Center, 14625 County Road 672, Wimauma, FL, 33598, USA.

Downy mildew (DM), caused by obligate parasitic oomycetes, is a destructive disease for a wide range of crops worldwide. Recent outbreaks of impatiens downy mildew (IDM) in many countries have caused huge economic losses. A system to reveal plant-pathogen interactions in the early stage of infection and quickly assess resistance/susceptibility of plants to DM is desired. Read More

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Comparative transcriptomic analysis highlights contrasting levels of resistance of Vitis vinifera and Vitis amurensis to Botrytis cinerea.

Hortic Res 2021 May 1;8(1):103. Epub 2021 May 1.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, 712100, Yangling, Xianyang, Shaanxi, China.

Botrytis cinerea is a major grapevine (Vitis spp.) pathogen, but some genotypes differ in their degree of resistance. For example, the Vitis vinifera cultivar Red Globe (RG) is highly susceptible, but V. Read More

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Secondary Metabolism and Defense Responses Are Differently Regulated in Two Grapevine Cultivars during Ripening.

Int J Mol Sci 2021 Mar 17;22(6). Epub 2021 Mar 17.

Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino, Italy.

'Nebbiolo' is one of the most important wine grape cultivars used to produce prestigious high-quality wines known throughout the world, such as Barolo and Barbaresco. 'Nebbiolo' is a distinctive genotype characterized by medium/high vigor, long vegetative and ripening cycles, and limited berry skin color rich in 3'-hydroxylated anthocyanins. To investigate the molecular basis of these characteristics, 'Nebbiolo' berries collected at three different stages of ripening (berry pea size, véraison, and harvest) were compared with 'Barbera' berries, which are rich in 3',5'-hydroxylated anthocyanins, using transcriptomic and analytical approaches. Read More

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Biosynthesis of the Sex Pheromone Component (E,Z)-7,9-Dodecadienyl Acetate in the European Grapevine Moth, Lobesia botrana, Involving ∆11 Desaturation and an Elusive ∆7 Desaturase.

J Chem Ecol 2021 Mar 29;47(3):248-264. Epub 2021 Mar 29.

Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden.

The European grapevine moth, Lobesia botrana, uses (E,Z)-7,9-dodecadienyl acetate as its major sex pheromone component. Through in vivo labeling experiments we demonstrated that the doubly unsaturated pheromone component is produced by ∆11 desaturation of tetradecanoic acid, followed by chain shortening of (Z)-11-tetradecenoic acid to (Z)-9-dodecenoic acid, and subsequently introduction of the second double bond by an unknown ∆7 desaturase, before final reduction and acetylation. By sequencing and analyzing the transcriptome of female pheromone glands of L. Read More

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Overexpression of VlPRX21 and VlPRX35 genes in Arabidopsis plants leads to bioconversion of trans-resveratrol to δ-viniferin.

Plant Physiol Biochem 2021 May 12;162:556-563. Epub 2021 Mar 12.

Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212, Republic of Korea. Electronic address:

Stilbenes, including resveratrol and viniferins, a small family of polyphenols, are considered the most important phytoalexin group in Vitis species. In a previous study, we found that co-treatment of methyl jasmonate (MJ) and stevioside (STE) resulted in enhanced extracellular production of viniferins in grapevine cell suspension cultures. Thus, to further understand the mechanisms of viniferin production in grapevine cell cultures, we performed transcriptome analysis and isolated seven candidates of grapevine peroxidase genes (VlAPX6, VlGPX5, VlPRX13, VlPRX21, VlPRX35, VlPRX40, and VlPRX50). Read More

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Genome-wide identification of BAM genes in grapevine (Vitis vinifera L.) and ectopic expression of VvBAM1 modulating soluble sugar levels to improve low-temperature tolerance in tomato.

BMC Plant Biol 2021 Mar 26;21(1):156. Epub 2021 Mar 26.

College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People's Republic of China.

Background: Low temperature (LT) is one of the main limiting factors that affect growth and development in grape. Increasing soluble sugar and scavenging reactive oxygen species (ROS) play critical roles in grapevine resistance to cold stress. However, the mechanism of β-amylase (BAM) involved in the regulation of sugar levels and antioxidant enzyme activities in response to cold stress is unclear. Read More

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GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response.

Plant Physiol 2021 Mar 22. Epub 2021 Mar 22.

Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Innovation Academy for Seed Design, the Chinese Academy of Science, Beijing 100093, P.R. China.

Cultivated grapevine (Vitis) is a highly valued horticultural crop, and cold stress affects its growth and productivity. Wild Amur grape (Vitis amurensis) PAT1 (Phytochrome A signal transduction 1, VaPAT1) is induced by low temperature, and ectopic expression of VaPAT1 enhances cold tolerance in Arabidopsis (Arabidopsis thaliana). However, little is known about the molecular mechanism of VaPAT1 during the cold stress response in grapevine. Read More

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Ethephon Activates the Transcription of Senescence-Associated Genes and Nitrogen Mobilization in Grapevine Leaves ( cv. Riesling).

Plants (Basel) 2021 Feb 9;10(2). Epub 2021 Feb 9.

Department of Soil Science and Plant Nutrition, Hochschule Geisenheim University, Von-Lade-Str. 1, D-65366 Geisenheim, Germany.

Nitrogen (N) remobilization in the context of leaf senescence is of considerable importance for the viability of perennial plants. In late-ripening crops, such as , it may also affect berry ripening and fruit quality. Numerous studies on the model plant have confirmed an involvement of the plant hormone ethylene in the regulation of senescence. Read More

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February 2021

VviNAC33 promotes organ de-greening and represses vegetative growth during the vegetative-to-mature phase transition in grapevine.

New Phytol 2021 07 16;231(2):726-746. Epub 2021 Mar 16.

Department of Biotechnology, University of Verona, Verona, 37134, Italy.

Plants undergo several developmental transitions during their life cycle. In grapevine, a perennial woody fruit crop, the transition from vegetative/green-to-mature/woody growth involves transcriptomic reprogramming orchestrated by a small group of genes encoding regulators, but the underlying molecular mechanisms are not fully understood. We investigated the function of the transcriptional regulator VviNAC33 by generating and characterizing transgenic overexpressing grapevine lines and a chimeric repressor, and by exploring its putative targets through a DNA affinity purification sequencing (DAP-seq) approach combined with transcriptomic data. Read More

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Comparative transcriptome analyses between cultivated and wild grapes reveal conservation of expressed genes but extensive rewiring of co-expression networks.

Plant Mol Biol 2021 May 4;106(1-2):1-20. Epub 2021 Feb 4.

Embrapa Uva e Vinho (Brazilian Agricultural Research Corporation, Grape and Wine Research Center), Rua Livramento, 515, Bento Gonçalves, RS, 95701-008, Brazil.

Key Message: The transcriptomes of wild and cultivated grapes consists of similar expressed genes but distinct wiring of co-expressed modules associated with environmental conditions. Grapevine is an important fruit crop worldwide, with high economic value and widespread distribution. Commercial production is based on Vitis vinifera, and, to a lesser extent, on hybrids with American grapes, such as V. Read More

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Transcriptomic analysis of grapevine Dof transcription factor gene family in response to cold stress and functional analyses of the VaDof17d gene.

Planta 2021 Feb 1;253(2):55. Epub 2021 Feb 1.

Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Innovation Academy for Seed Design, Chinese Academy of Science, Beijing, 100093, People's Republic of China.

Main Conclusion: Dof genes enhance cold tolerance in grapevine and VaDof17d is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides. DNA-binding with one finger (Dof) proteins comprise a large family that plays important roles in the regulation of abiotic stresses. No in-depth analysis of Dof genes has been performed in the grapevine. Read More

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February 2021

Transcriptome Analysis and Cell Morphology of Cells to Botryosphaeria Dieback Pathogen .

Genes (Basel) 2021 01 27;12(2). Epub 2021 Jan 27.

College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China.

, one of the major causal agents of Botryosphaeria dieback, spreads worldwide causing cankers, leaf spots and fruit black rot in grapevine. is an American wild grapevine widely used for resistance and rootstock breeding and was found to be highly resistant to Botryosphaeria dieback. The defence responses of to 98. Read More

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January 2021

Genome Assembly and Transcriptome Analysis of the Fungus During Infection on Grapevine ( L.).

Front Microbiol 2020 11;11:599150. Epub 2021 Jan 11.

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.

Grape white rot caused by (Speg.) affects the production and quality of grapevine in China and other grapevine-growing countries. Despite the importance of as a serious disease-causing agent in grape, the genome information and molecular mechanisms underlying its pathogenicity are poorly understood. Read More

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January 2021

The Grapevine E3 Ubiquitin Ligase VriATL156 Confers Resistance against the Downy Mildew Pathogen .

Int J Mol Sci 2021 Jan 19;22(2). Epub 2021 Jan 19.

Department of Biotechnology, University of Verona, Strada Le Grazie 15, CV1, 37134 Verona, Italy.

Downy mildew, caused by , is one of the most severe diseases of grapevine ( L.). Genetic resistance is an effective and sustainable control strategy, but major resistance genes (encoding receptors for specific pathogen effectors) introgressed from wild species, although effective, may be non-durable because the pathogen can evolve to avoid specific recognition. Read More

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January 2021

The genome of Shanputao (Vitis amurensis) provides a new insight into cold tolerance of grapevine.

Plant J 2021 03 21;105(6):1495-1506. Epub 2021 Jan 21.

Beijing Key Laboratory of Grape Science and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Innovation Academy for Seed Design, the Chinese Academy of Science, Beijing, 100093, China.

Vitis amurensis (Shanputao) is the most cold tolerant Vitis species and so is of great interest to grape breeders and producers in areas with low winter temperatures. Here, we report its high-quality, chromosome-level genome assembly based on a combination of sequence data from Illumina and PacBio platforms, BioNano optical mapping and high-throughput chromosome conformation Capture (Hi-C) mapping. The 604. Read More

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Xylella fastidiosa causes transcriptional shifts that precede tylose formation and starch depletion in xylem.

Mol Plant Pathol 2021 02 20;22(2):175-188. Epub 2020 Nov 20.

Department of Microbiology and Plant Pathology, University of California, Riverside, California, USA.

Pierce's disease (PD) in grapevine (Vitis vinifera) is caused by the bacterial pathogen Xylella fastidiosa. X. fastidiosa is limited to the xylem tissue and following infection induces extensive plant-derived xylem blockages, primarily in the form of tyloses. Read More

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February 2021

Evaluation of RNA Interference for Control of the Grape Mealybug (Hemiptera: Pseudococcidae).

Insects 2020 Oct 28;11(11). Epub 2020 Oct 28.

Department of Entomology, Cornell University, Ithaca, NY 14850, USA.

The grape mealybug (Ehrhorn, 1900) (Hemiptera: Pseudococcidae) is a significant pest of grapevines ( spp.) and a vector of disease-causing grape viruses, linked to its feeding on phloem sap. The management of this pest is constrained by the lack of naturally occurring resistance traits in . Read More

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October 2020

The Biocontrol Root-Oomycete, , Triggers Grapevine Resistance and Shifts in the Transcriptome of the Trunk Pathogenic Fungus, .

Int J Mol Sci 2020 Sep 19;21(18). Epub 2020 Sep 19.

INRAE, UMR 1065 Santé et Agroécologie du Vignoble (SAVE), Institut des Sciences de la vigne et du Vin (ISVV), 33883 Villenave d'Ornon, France.

The worldwide increase in grapevine trunk diseases, mainly esca, represents a major threat for vineyard sustainability. Biocontrol of a pioneer fungus of esca, , was investigated here by deciphering the tripartite interaction between this trunk-esca pathogen, grapevine and the biocontrol-oomycete, . When colonizes grapevine roots, it was observed that the wood necroses caused by were significantly reduced. Read More

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September 2020

The Molecular Priming of Defense Responses is Differently Regulated in Grapevine Genotypes Following Elicitor Application against Powdery Mildew.

Int J Mol Sci 2020 Sep 15;21(18). Epub 2020 Sep 15.

Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino, Italy.

Molecular changes associated with response to powdery mildew (PM) caused by have been largely explored in cultivars, but little is known on transcriptional and metabolic modifications following application of resistance elicitors against this disease. In this study, the whole transcriptome sequencing, and hormone and metabolite analyses were combined to dissect long-term defense mechanisms induced by molecular reprogramming events in PM-infected 'Moscato' and 'Nebbiolo' leaves treated with three resistance inducers: acibenzolar-S-methyl, potassium phosphonate, and laminarin. Although all compounds were effective in counteracting the disease, acibenzolar-S-methyl caused the most intense transcriptional modifications in both cultivars. Read More

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September 2020