115,509 results match your criteria transcriptional response

Midgut transcriptome assessment of the cockroach-hunting wasp Ampulex compressa (Apoidea: Ampulicidae).

PLoS One 2021 24;16(6):e0252221. Epub 2021 Jun 24.

Evolutionary Biology and Ecology, Institute of Biology I (Zoology), Albert-Ludwig University of Freiburg, Freiburg, Germany.

The emerald jewel wasp Ampulex compressa (Hymenoptera: Ampulicidae) is a solitary wasp that is widely known for its specialized hunting of cockroaches as larvae provision. Adult wasps mainly feed on pollen and nectar, while their larvae feed on the cockroachs' body, first as ecto- and later as endoparsitoids. Little is known about the expression of digestive, detoxification and stress-response-related genes in the midgut of A. Read More

View Article and Full-Text PDF

Egr‑1 inhibits colon cancer cell proliferation, migration and invasion via regulating CDKL1 at the transcriptional level.

Oncol Rep 2021 Aug 24;46(2). Epub 2021 Jun 24.

Department of Anus and Intestine Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.

Colon cancer is one of the most common malignant tumors worldwide, and the molecular mechanisms involved in the oncogenesis and progression of colon cancer remain unclear. Early growth response 1 (Egr‑1) is a transcription factor that is closely associated with several tumor processes; however, its role in colon cancer is unknown. The present study aimed to explore the function and mechanism of transcription factor Egr‑1 in colon cancer progression. Read More

View Article and Full-Text PDF

BBX19 fine-tunes the circadian rhythm by interacting with PSEUDO-RESPONSE REGULATOR proteins to facilitate their repressive effect on morning-phased clock genes.

Plant Cell 2021 May 14. Epub 2021 May 14.

State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475004, China.

The core plant circadian oscillator is composed of multiple interlocked transcriptional-translational feedback loops, which synchronize endogenous diel physiological rhythms to the cyclic changes of environmental cues. PSEUDO-RESPONSE REGULATORS (PRRs) have been identified as negative components in the circadian clock, though their underlying molecular mechanisms remain largely unknown. Here, we found that a subfamily of zinc finger transcription factors, B-box (BBX)-containing proteins, have a critical role in fine-tuning circadian rhythm. Read More

View Article and Full-Text PDF

Hooked on heart regeneration: the zebrafish guide to recovery.

Cardiovasc Res 2021 Jun 23. Epub 2021 Jun 23.

Centre for Cardiovascular Science, University of Edinburgh, UK.

While humans lack sufficient capacity to undergo cardiac regeneration following injury, zebrafish can fully recover from a range of cardiac insults. Over the past two decades our understanding of the complexities of both the independent and co-ordinated injury responses by multiple cardiac tissues during zebrafish heart regeneration has increased exponentially. Although cardiomyocyte regeneration forms the cornerstone of the reparative process in the injured zebrafish heart, recent studies have shown that this is dependent on prior neovascularisation and lymphangiogenesis, which in turn require epicardial, endocardial and inflammatory cell signalling within an extracellular milieu that is optimised for regeneration. Read More

View Article and Full-Text PDF

Genetic Signatures From RNA Sequencing of Pediatric Localized Scleroderma Skin.

Front Pediatr 2021 7;9:669116. Epub 2021 Jun 7.

Division of Rheumatology, Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA, United States.

The purpose of this study was to explore the skin transcriptional profile in pediatric localized scleroderma (LS) to provide a better understanding of the altered immune and fibrotic pathways promoting disease. LS is a progressive disease of the skin and underlying tissue that causes significant functional disability and disfigurement, especially in developing children. RNA sequencing (RNAseq) technology allows for improved understanding of relevant cellular expression through transcriptome analysis of phases during LS disease progression (more active/inflammatory vs. Read More

View Article and Full-Text PDF

Synthesis of 2-oxoglutarate derivatives and their evaluation as cosubstrates and inhibitors of human aspartate/asparagine-β-hydroxylase.

Chem Sci 2020 Dec 7;12(4):1327-1342. Epub 2020 Dec 7.

Chemistry Research Laboratory, University of Oxford 12 Mansfield Road OX1 3TA Oxford UK

2-Oxoglutarate (2OG) is involved in biological processes including oxidations catalyzed by 2OG oxygenases for which it is a cosubstrate. Eukaryotic 2OG oxygenases have roles in collagen biosynthesis, lipid metabolism, DNA/RNA modification, transcriptional regulation, and the hypoxic response. Aspartate/asparagine-β-hydroxylase (AspH) is a human 2OG oxygenase catalyzing post-translational hydroxylation of Asp/Asn-residues in epidermal growth factor-like domains (EGFDs) in the endoplasmic reticulum. Read More

View Article and Full-Text PDF
December 2020

Poly adenosine diphosphate-ribosylation, a promising target for colorectal cancer treatment.

World J Gastrointest Oncol 2021 Jun;13(6):574-588

Research and Development, Metimedi Pharmaceuticals, Incheon 22006, South Korea.

The development of colorectal cancer (CRC) can result from changes in a variety of cellular systems within the tumor microenvironment. Particularly, it is primarily associated with genomic instability that is the gradual accumulation of genetic and epigenetic changes consisting of a characteristic set of mutations crucial for pathways in CRC progression. Based on this background, the potential to focus on poly [adenosine diphosphate (ADP)-ribose] polymerase (PARP)-1 and poly-ADP ribosylation (PARylation) as the main causes of malignant formation of CRC may be considered. Read More

View Article and Full-Text PDF

Evaluation and Differential Diagnosis of a Genetic Marked Brucella Vaccine A19ΔvirB12 for Cattle.

Front Immunol 2021 7;12:679560. Epub 2021 Jun 7.

Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, China.

is an important zoonotic pathogen that causes severe economic loss to husbandry and poses a threat to human health. The A19 live vaccine has been extensively used to prevent bovine brucellosis in China. However, it is difficult to distinguish the serological response induced by A19 from that induced by natural infection. Read More

View Article and Full-Text PDF

Syncytiotrophoblast Extracellular Vesicles From Late-Onset Preeclampsia Placentae Suppress Pro-Inflammatory Immune Response in THP-1 Macrophages.

Front Immunol 2021 7;12:676056. Epub 2021 Jun 7.

Nuffield Department of Women's and Reproductive Health, University of Oxford, Oxford, United Kingdom.

Syncytiotrophoblast derived Extracellular Vesicles (STBEV) from normal pregnancy (NP) have previously been shown to interact with circulating monocytes and B cells and induce pro-inflammatory cytokine release. Early-onset preeclampsia (EOPE) is associated with an exacerbated inflammatory response, yet there is little data regarding late-onset PE (LOPE) and immune function. Here, using a macrophage/monocyte cell line THP-1, we investigated the inflammatory potential of STBEV, comprising medium/large-STBEV (>200nm) and small-STBEV (<200nm), isolated from LOPE (n=6) and normal (NP) (n=6) placentae via dual-lobe placental perfusion and differential centrifugation. Read More

View Article and Full-Text PDF

A transcriptional switch governs fibroblast activation in heart disease.

Nature 2021 Jun 23. Epub 2021 Jun 23.

Gladstone Institutes, San Francisco, CA, USA.

In diseased organs, stress-activated signalling cascades alter chromatin, thereby triggering maladaptive cell state transitions. Fibroblast activation is a common stress response in tissues that worsens lung, liver, kidney and heart disease, yet its mechanistic basis remains unclear. Pharmacological inhibition of bromodomain and extra-terminal domain (BET) proteins alleviates cardiac dysfunction, providing a tool to interrogate and modulate cardiac cell states as a potential therapeutic approach. Read More

View Article and Full-Text PDF

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Oncogene 2021 Jun 23. Epub 2021 Jun 23.

State Key Laboratory of Oncogenes and Related Genes, Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Pancreatic ductal adenocarcinoma (PDAC) is the deadliest cancer mainly owing to its proclivity to early metastasis and the lack of effective targeted therapeutic drugs. Hence, understanding the molecular mechanisms underlying early invasion and metastasis by PDAC is imperative for improving patient outcomes. The present study identified that upregulation of TSPAN8 expression in PDAC facilitates metastasis in vivo and in vitro. Read More

View Article and Full-Text PDF

Gene expression profiles during tissue remodeling following bladder outlet obstruction.

Sci Rep 2021 Jun 23;11(1):13171. Epub 2021 Jun 23.

Department of Urology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto-City, Kyoto, 602-8566, Japan.

Bladder outlet obstruction (BOO) often results in lower urinary tract symptoms (LUTSs) and negatively affects quality of life. Here, we evaluated gene expression patterns in the urinary bladder during tissue remodeling due to BOO. We divided BOO model rats into two groups according to the degree of hypertrophy of smooth muscle in the bladder. Read More

View Article and Full-Text PDF

Ischemic heart injury leads to HIF1-dependent differential splicing of CaMK2γ.

Sci Rep 2021 Jun 23;11(1):13116. Epub 2021 Jun 23.

Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawaii, 651 Ilalo St. BSB 311, Honolulu, HI, 96813, USA.

Ischemic heart disease is a leading cause of heart failure and hypoxia inducible factor 1 (HIF1) is a key transcription factor in the response to hypoxic injury. Our lab has developed a mouse model in which a mutated, oxygen-stable form of HIF1α (HIF-PPN) can be inducibly expressed in cardiomyocytes. We observed rapid cardiac dilation and loss of contractility in these mice due to lower expression of excitation-contraction coupling genes and reduced calcium flux. Read More

View Article and Full-Text PDF

The circadian clock is associated with prognosis and immune infiltration in stomach adenocarcinoma.

Aging (Albany NY) 2021 Jun 23;13. Epub 2021 Jun 23.

Department of Emergency, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, China.

Background: Stomach adenocarcinoma (STAD) is one of the most prevalent malignances and ranks fifth in incidence and third in cancer-related death among all malignances. The prognosis of STAD is poor. The circadian clock is regulated by interlocked transcriptional-translational feedback loops that orchestrate circadian rhythms in some biological processes, including the immune response and metabolism. Read More

View Article and Full-Text PDF

Integrative Cistromic and Transcriptomic Analyses Identify CREB Target Genes in Cystic Renal Epithelial Cells.

J Am Soc Nephrol 2021 Jun 23. Epub 2021 Jun 23.

Y Chen, 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Institute of Urology, Tianjin, China

Genome-wide mapping of transcription factor (TF) binding sites is essential to identify a TF's direct target genes in kidney development and diseases. However, due to the cellular complexity of the kidney and limited numbers of a given cell type, it has been challenging to determine the binding sites of a TF in vivo. cAMP-response element-binding protein (CREB) is phosphorylated and hyperactive in autosomal dominant polycystic kidney disease (ADPKD). Read More

View Article and Full-Text PDF

Endothelial Recovery in Bare Metal Stents and Drug-Eluting Stents on a Single-Cell Level.

Arterioscler Thromb Vasc Biol 2021 Jun 24:ATVBAHA121316472. Epub 2021 Jun 24.

CVPath Institute, Gaithersburg, MD (A.C., L.G., R.F., C.J., S.K., A.S., H.J., Y.S., K.H.P., F.D.K., M.E.R., D.S., R.V., A.V.F.).

Objective: Healing processes, particularly reendothelialization, are essential for vascular homeostasis after plain old balloon angioplasty and stent implantation. Drug-eluting stents (DES) are commonly used for percutaneous coronary intervention because restenosis rates are reduced as compared with bare metal stents. However, in addition to understanding the nature of regenerated endothelial cells, concerns over incomplete stent healing persist, and the molecular effects of antiproliferative drug coatings on endothelium remain poorly understood. Read More

View Article and Full-Text PDF

Response and resistance to CDK12 inhibition in aggressive B-cell lymphomas.

Haematologica 2021 Jun 24. Epub 2021 Jun 24.

Department of Hematopathology and Laboratory Medicine, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, 33612.

Despite significant progress in the treatment of patients with diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL), prognosis of patients with relapsed disease remains poor due to the emergence of drug resistance and subsequent disease progression. Identification of novel targets and therapeutic strategies for these diseases represents an urgent need. Here, we report that both MCL and DLBCL are exquisitely sensitive to transcription-targeting drugs, particular to THZ531, a covalent inhibitor of cyclin-dependent kinase 12 (CDK12). Read More

View Article and Full-Text PDF

Global transcriptome profiling reveals antagonizing response of head kidney of juvenile common carp exposed to glyphosate.

Chemosphere 2021 Oct 10;280:130823. Epub 2021 May 10.

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Tai'an City, Shandong Province, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Tai'an City, Shandong Province, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Tai'an City, Shandong Province, 271018, China. Electronic address:

Glyphosate (GLY) frequently detected in various water bodies has imposed a serious risk on fish. Head kidney of fish is an important defense organ, playing a vital part in antagonizing exogenous hazardous matter. The objective of this study was to characterize toxic mechanisms of GLY in head kidney of common carp based on transcriptome profiling. Read More

View Article and Full-Text PDF
October 2021

Invasion history shapes host transcriptomic response to a body-snatching parasite.

Mol Ecol 2021 Jun 23. Epub 2021 Jun 23.

Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.

By shuffling biogeographic distributions, biological invasions can both disrupt long-standing associations between hosts and parasites and establish new ones. This creates natural experiments with which to study the ecology and evolution of host-parasite interactions. In estuaries of the Gulf of Mexico, the white-fingered mud crab (Rhithropanopeus harrisii) is infected by a native parasitic barnacle Loxothylacus panopaei (Rhizocephala), which manipulates host physiology and behavior. Read More

View Article and Full-Text PDF

Single-cell BCR and transcriptome analysis after influenza infection reveals spatiotemporal dynamics of antigen-specific B cells.

Cell Rep 2021 Jun;35(12):109286

Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden. Electronic address:

B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of antigen-specific cells in lymph nodes, spleen, and lungs after influenza infection in mice, we identify several germinal center (GC) B cell subpopulations and organ-specific differences that persist over the course of the response. Read More

View Article and Full-Text PDF

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Cell Rep 2021 Jun;35(12):109281

Shanghai Key Laboratory of Metabolic Remodeling and Disease, Institute of Metabolism and Integrative Biology, Zhongshan Hospital, Fudan University, and Shanghai Qi Zhi Institute, Shanghai, China; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China. Electronic address:

Obesity has become a global pandemic. Identification of key factors in adipogenesis helps to tackle obesity and related metabolic diseases. Here, we show that DDB1 binds the histone reader BRWD3 to promote adipogenesis and diet-induced obesity. Read More

View Article and Full-Text PDF

Unveiling sugarcane defense response to Mythimna separata herbivory by combination of transcriptome and metabolic analyses.

Pest Manag Sci 2021 Jun 23. Epub 2021 Jun 23.

National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, 350002, P. R. China.

Background: Sugarcane is the most important sugar crops in the world. Like other crops, sugarcane is also suffered from herbivorous insect attack. The oriental armyworm Mythimna separata is a devastating pest of various crop in northeast Asia, outbreak of this pest resulted in substantially yield loss including sugarcane. Read More

View Article and Full-Text PDF

Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes.

PLoS One 2021 23;16(6):e0253475. Epub 2021 Jun 23.

Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Medicine, McGill University, Montreal, Quebec, Canada.

Hepcidin is a peptide hormone that targets the iron exporter ferroportin, thereby limiting iron entry into the bloodstream. It is generated in hepatocytes mainly in response to increased body iron stores or inflammatory cues. Iron stimulates expression of bone morphogenetic protein 6 (BMP6) from liver sinusoidal endothelial cells, which in turn binds to BMP receptors on hepatocytes and induces the SMAD signaling cascade for transcriptional activation of the hepcidin-encoding HAMP mRNA. Read More

View Article and Full-Text PDF

Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosoma brucei.

PLoS Pathog 2021 Jun 23;17(6):e1009696. Epub 2021 Jun 23.

Department of Life Sciences, Sir Ernst Chain Building, Imperial College London, London, United Kingdom.

Iron is an essential regulatory signal for virulence factors in many pathogens. Mammals and bloodstream form (BSF) Trypanosoma brucei obtain iron by receptor-mediated endocytosis of transferrin bound to receptors (TfR) but the mechanisms by which T. brucei subsequently handles iron remains enigmatic. Read More

View Article and Full-Text PDF

The Heat Shock Repressor HspR Directly Controls Avermectin Production, Morphological Development, and HO Stress Response in .

Appl Environ Microbiol 2021 Jun 23:AEM0047321. Epub 2021 Jun 23.

State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, China.

Heat shock response (HSR) is a universal cellular response that promotes survival following temperature increase. In filamentous , which account for ∼70% commercial antibiotic production, HSR is regulated by transcriptional repressors; in particular, the widespread MerR-family regulator HspR has been identified as a key repressor. However, functions of HspR in other biological processes are unknown. Read More

View Article and Full-Text PDF

Variations in transfer and ribosomal RNA epitranscriptomic status can adapt eukaryote translation to changing physiological and environmental conditions.

RNA Biol 2021 Jun 23:1-15. Epub 2021 Jun 23.

CNRS LGDP-UMR5096, Alduy France.

The timely reprogramming of gene expression in response to internal and external cues is essential to eukaryote development and acclimation to changing environments. Chemically modifying molecular receptors and transducers of these signals is one way to efficiently induce proper physiological responses. Post-translation modifications, regulating protein biological activities, are central to many well-known signal-responding pathways. Read More

View Article and Full-Text PDF

miRNAs play critical roles in response to abiotic stress by modulating cross-talk of phytohormone signaling.

Plant Cell Rep 2021 Jun 22. Epub 2021 Jun 22.

Molecular Biology and Biotechnology Division, CSIR-National Botanical Research Institute, Lucknow, India.

One of the most interesting signaling molecules that regulates a wide array of adaptive stress responses in plants are the micro RNAs (miRNAs) that are a unique class of non-coding RNAs constituting novel mechanisms of post-transcriptional gene regulation. Recent studies revealed the role of miRNAs in several biotic and abiotic stresses by regulating various phytohormone signaling pathways as well as by targeting a number of transcription factors (TFs) and defense related genes. Phytohormones are signal molecules modulating the plant growth and developmental processes by regulating gene expression. Read More

View Article and Full-Text PDF

Spike-in normalization for single-cell RNA-seq reveals dynamic global transcriptional activity mediating anticancer drug response.

NAR Genom Bioinform 2021 Jun 17;3(2):lqab054. Epub 2021 Jun 17.

Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

The transcriptional plasticity of cancer cells promotes intercellular heterogeneity in response to anticancer drugs and facilitates the generation of subpopulation surviving cells. Characterizing single-cell transcriptional heterogeneity after drug treatments can provide mechanistic insights into drug efficacy. Here, we used single-cell RNA-seq to examine transcriptomic profiles of cancer cells treated with paclitaxel, celecoxib and the combination of the two drugs. Read More

View Article and Full-Text PDF

Novel vectors and approaches for gene therapy in liver diseases.

JHEP Rep 2021 Aug 30;3(4):100300. Epub 2021 Apr 30.

Gene Therapy Area, Foundation for Applied Medical Research, University of Navarra, IdisNA, Pamplona, Spain.

Gene therapy is becoming an increasingly valuable tool to treat many genetic diseases with no or limited treatment options. This is the case for hundreds of monogenic metabolic disorders of hepatic origin, for which liver transplantation remains the only cure. Furthermore, the liver contains 10-15% of the body's total blood volume, making it ideal for use as a factory to secrete proteins into the circulation. Read More

View Article and Full-Text PDF

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Cell Death Differ 2021 Jun 22. Epub 2021 Jun 22.

Danish Cancer Society Research Center, Copenhagen, Denmark.

During autophagy, the coordinated actions of autophagosomes and lysosomes result in the controlled removal of damaged intracellular organelles and superfluous substrates. The evolutionary conservation of this process and its requirement for maintaining cellular homeostasis emphasizes the need to better dissect the pathways governing its molecular regulation. In our previously performed high-content screen, we assessed the effect of 1530 RNA-binding proteins on autophagy. Read More

View Article and Full-Text PDF