771,319 results match your criteria transcription block

Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway.

Vet Microbiol 2021 May 4;258:109098. Epub 2021 May 4.

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China. Electronic address:

Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection via immunosuppression. Although the co-infection of PCV2 and porcine parvovirus (PPV) is commonly observed in worldwide, the relative immune mechanisms promoting PPV infection in PCV2-infected piglets are currently unknown. Herein, we found that PCV2 infection suppressed IFN-β expression and promoted PPV infection in the piglets. Read More

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Direct reprogramming with Sendai virus vectors repaired infarct hearts at the chronic stage.

Biochem Biophys Res Commun 2021 May 10;560:87-92. Epub 2021 May 10.

Department of Cardiology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba City, Ibaraki, 305-8575, Japan. Electronic address:

Adult hearts have limited regenerative capacity. Hence, after acute myocardial infarction (MI), dead myocardial tissues are digested by immune cells and replaced by fibrosis, leading to ventricular remodeling and heart failure at the chronic stage. Direct reprogramming of the cardiac fibroblasts (CFs) into induced cardiomyocytes (iCMs) with cardiac transcription factors, including Gata4, Mef2c, and Tbx5 (GMT), may have significant potential for cardiac repair. Read More

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RNA polymerase I subunit 12 plays opposite roles in cell proliferation and migration.

Biochem Biophys Res Commun 2021 May 10;560:112-118. Epub 2021 May 10.

College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, 430065, China. Electronic address:

RNA polymerase I (Pol I) is responsible for the synthesis of the majority of ribosomal RNA molecules in eukaryotes. Pol I subunit 12 (RPA12) is involved in the transcriptional termination and lipid metabolism in yeast. However, its role in human cells hasn't been investigated so far. Read More

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Expression pattern of transcriptional enhanced associate domain family member 1 (Tead1) in developing mouse molar tooth.

Gene Expr Patterns 2021 May 10:119182. Epub 2021 May 10.

Department of Maxillofacial Orthognathics, Division of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.

The Hippo pathway is essential for determining organ size by regulating cell proliferation. Previous reports have shown that impairing this pathway causes abnormal tooth development. However, the precise expression profile of the members of the transcriptional enhanced associate domain family (Tead), which are key transcription factors mediating Yap function, during tooth development is unclear. Read More

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Epigenetic Regulation of Melanogenesis.

Ageing Res Rev 2021 May 10:101349. Epub 2021 May 10.

Department of Dermatology, Third Xiangya Hospital, Central South University, 138 Tongzipo Road, Changsha, Hunan, 410013, PR China. Electronic address:

Melanogenesis is a complex process in which melanin is synthesized in melanocytes and transported to keratinocytes, which involves multiple genes and signaling pathways. Epigenetics refers to the potential genetic changes that affect gene expression without involving changes in the original sequence of DNA nucleotides. DNA methylation regulates the expression of key genes such as tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), dopachrome tautomerase (DCT) and microphthalmia-associated transcription factor (MITF), as well as paracrine factors such as stem cell factor (SCF) and endothelin-1 (ET-1) in melanogenesis. Read More

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The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli.

N Biotechnol 2021 May 10. Epub 2021 May 10.

Institute of Microbiology and Infection and School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address:

Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins that are difficult to fold, targeted to the membrane or secreted out of the cytoplasm. To circumvent this problem, a suite of bacterial promoters has been constructed with a range of different promoter strengths, assigning them specific "promoter activity ratings" (PARs). Read More

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Network pharmacology integrated with experimental validation reveals the regulatory mechanism of plastrum testudinis in treating senile osteoporosis.

J Ethnopharmacol 2021 May 10:114198. Epub 2021 May 10.

The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China; Lingnan Medical Research Center of Guangzhou Univercity of Chinese Medicine, Guangzhou, 510405, China. Electronic address:

Ethnopharmacological Relevance: Plastrum testudinis (PT) has been used in traditional Chinese medicine to treat bone diseases such as senile osteoporosis (SOP) for thousands of years. However, the underlying mechanisms remain largely unknown.

Aim Of The Study: This study aims to investigate the possible molecular mechanism of PT in the treatment of SOP using an integrated strategy of network pharmacology and experimental validation. Read More

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Discovery of BrATG6 and its potential role in Brassica rapa L. resistance to infection by Plasmodiophora brassicae.

Gene 2021 May 10:145711. Epub 2021 May 10.

Shenyang Agricultural University, Liaoning province cruciferous vegetables genetic breeding primary laboratory, Shenyang, Liaoning, 110866, China. Electronic address:

Clubroot disease, caused by Plasmodiophora brassicae infection, occurs in cruciferous vegetable crops in many areas of the world, sometimes leading to yield loss. In this study, a differentially expressed protein (0305), was found between control and clubroot-diseased Chinese cabbage (Brassica rapa L.) roots through two-dimensional electrophoresis. Read More

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Degradation of p53 by HPV16-E6 variants isolated from cervical cancer specimens of Moroccan women.

Gene 2021 May 10:145709. Epub 2021 May 10.

Biology and Medical Research Unit, National Centre for Energy, Nuclear Sciences and Techniques (CNESTEN), Rabat, Morocco. Electronic address:

Cervical cancer is the second most diagnosed cancer in Moroccan women. The main etiological factor for developing cervical cancer is the persistent infection with HPV16. Genetic studies have reported the occurrence of amino acid variations within the E6 oncoprotein that promotes host cell transformation by targeting p53 for degradation. Read More

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Diphlorethohydroxycamalol isolated from Ishige okamurae prevents HO-induced oxidative damage via BMP2/Runx2 signaling in osteoblastic MC3T3-E1 cells.

Fitoterapia 2021 May 10:104921. Epub 2021 May 10.

Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea. Electronic address:

Accumulating evidence has shown an association between osteoporosis and oxidative damage. In the present study, the protective effects of diphlorethohydroxycarmalol (DPHC) isolated from the brown algae Ishige okamurae against HO-induced oxidative damage via BMP2/ runt-related transcription factor 2 (Runx2) signaling were investigated using MC3T3-E1 osteoblastic cells. DPHC counteracted the reduction in cell viability caused by HO exposure and protected against HO-induced dysfunction, demonstrated by improved cellular alkaline phosphatase (ALP) activity and calcium deposition. Read More

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The binding of LDN193189 to CD133 C-terminus suppresses the tumorigenesis and immune escape of liver tumor-initiating cells.

Cancer Lett 2021 May 10. Epub 2021 May 10.

NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, PR China. Electronic address:

The tumor-initiating cell (TIC) marker CD133 promotes TIC self-renewal and tumorigenesis through the tyrosine phosphorylation of its c-terminal domain. Therefore, finding compounds that target the phosphorylation of CD133 will provide an effective method for inhibiting TICs characteristics. Here, through small molecule microarray screening, compound LDN193189 was found to bind to the c-terminus of CD133 and influenced its tyrosine phosphorylation. Read More

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Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay.

J Virol Methods 2021 May 10:114182. Epub 2021 May 10.

Institute for Medical Virology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, Germany. Electronic address:

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic requires fast and accurate high-throughput diagnostic tools. To evaluate the analytical performance of the Hologic Aptima transcription-mediated amplification (TMA) assay for detection of SARS-CoV-2 RNA from respiratory samples we analysed 103 clinical and proficiency panel samples pre-tested by real-time RT-PCR (Altona, RealStar) and found a positive percent agreement (sensitivity) of 95.7% and a negative percent agreement (specificity) of 100%. Read More

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The polyamine regulator AMD1 up-regulates spermine levels to drive epidermal differentiation.

J Invest Dermatol 2021 May 10. Epub 2021 May 10.

Maintaining tissue homeostasis depends on a balance of cell proliferation, differentiation and apoptosis. Within the epidermis the levels of the polyamines putrescine, spermidine and spermine are altered in many different skin conditions yet their role in epidermal tissue homeostasis is poorly understood. We identify the polyamine regulator, AMD1, as a crucial regulator of keratinocyte differentiation. Read More

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Fenofibrate, a PPARα agonist, reduces hepatic fat accumulation through the upregulation of TFEB-mediated lipophagy.

Metabolism 2021 May 10:154798. Epub 2021 May 10.

Department of Endocrinology and Metabolism, Kyung Hee University Hospital at Gangdong, Seoul, Republic of Korea; Division of Endocrinology and Metabolism, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Republic of Korea. Electronic address:

Background: Recent studies have shown that dysregulation of autophagy is involved in the development of nonalcoholic fatty liver disease (NAFLD). Transcription factors E3 (TFE3) and EB (TFEB) are master regulators of the transcriptional response of basic cellular processes such as lysosomal biogenesis and autophagy. Here, we investigated the role of fenofibrate, a PPARα agonist, in promotion of intracellular lipid clearance by upregulation of TFEB/TFE3. Read More

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Silencing Hoxa2 reverses dexamethasone-induced dysfunction of MC3T3-E1 osteoblasts and osteoporosis in rats.

Adv Clin Exp Med 2021 May 13. Epub 2021 May 13.

Department of Orthopedics, Liuzhou People's Hospital, Guangxi Medical University, China.

Background: Osteoporosis is damaging the health of women worldwide. Osteoporosis results from the imbalance between bone resorption and formation, which may be regulated by homeobox A2 (Hoxa2). However, the specific role and mechanism of Hoxa2 in osteogenesis and dexamethasone (Dex)-induced osteoporosis remain unknown. Read More

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Rhinocerebral Mucormycosis and COVID-19 Pneumonia.

J Med Cases 2021 Mar 19;12(3):85-89. Epub 2021 Jan 19.

Christiana Care Health System, 4755 Ogletown Stanton Road, Newark, DE 19718, USA.

As the coronavirus disease 2019 (COVID-19) pandemic is evolving, more complications associated with COVID-19 are emerging. In this case report, we present a case of rhinocerebral mucormycosis concurrent with COVID-19 pneumonia in a 41-year-old man with a history of type 1 diabetes mellitus (T1DM). COVID-19 pneumonia was diagnosed with reverse transcription-polymerase chain reaction (RT-PCR). Read More

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Multi-omics approach identifies germline regulatory variants associated with hematopoietic malignancies in retriever dog breeds.

PLoS Genet 2021 May 13;17(5):e1009543. Epub 2021 May 13.

Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

Histiocytic sarcoma is an aggressive hematopoietic malignancy of mature tissue histiocytes with a poorly understood etiology in humans. A histologically and clinically similar counterpart affects flat-coated retrievers (FCRs) at unusually high frequency, with 20% developing the lethal disease. The similar clinical presentation combined with the closed population structure of dogs, leading to high genetic homogeneity, makes dogs an excellent model for genetic studies of cancer susceptibility. Read More

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Diagnostic Performance of an Antigen Test with RT-PCR for the Detection of SARS-CoV-2 in a Hospital Setting - Los Angeles County, California, June-August 2020.

MMWR Morb Mortal Wkly Rep 2021 May 14;70(19):702-706. Epub 2021 May 14.

Prompt and accurate detection of SARS-CoV-2, the virus that causes COVID-19, has been important during public health responses for containing the spread of COVID-19, including in hospital settings (1-3). In vitro diagnostic nucleic acid amplification tests (NAAT), such as real-time reverse transcription-polymerase chain reaction (RT-PCR) can be expensive, have relatively long turnaround times, and require experienced laboratory personnel.* Antigen detection tests can be rapidly and more easily performed and are less expensive. Read More

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Community-Based Testing for SARS-CoV-2 - Chicago, Illinois, May-November 2020.

MMWR Morb Mortal Wkly Rep 2021 May 14;70(19):707-711. Epub 2021 May 14.

On May 13, 2020, Chicago established a free community-based testing (CBT) initiative for SARS-CoV-2, the virus that causes COVID-19, using reverse transcription-polymerase chain reaction (RT-PCR). The initiative focused on demographic groups and geographic areas that were underrepresented in testing by clinical providers and had experienced high COVID-19 incidence, including Hispanic persons and those who have been economically marginalized. To assess the CBT initiative, the Chicago Department of Public Health (CDPH) compared demographic characteristics, economic marginalization, and test positivity between persons tested at CBT sites and persons tested in all other testing settings in Chicago. Read More

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Integrative methylome-transcriptome analysis unravels cancer cell vulnerabilities in infant MLL-rearranged B-cell acute lymphoblastic leukemia.

J Clin Invest 2021 May 13. Epub 2021 May 13.

Josep Carreras Leukemia Research Institute-Campus Clinic, University of Barcelona, Barcelona, Spain.

B-cell acute lymphoblastic leukemia (B-ALL) is the most common childhood cancer. As predicated by its prenatal origin, infant B-ALL (iB-ALL) shows an exceptionally silent DNA mutational landscape, suggesting that alternative epigenetic mechanisms may substantially contribute to its leukemogenesis. Here, we have integrated genome-wide DNA methylome and transcriptome data from 69 patients with de novo MLL-rearranged (MLLr) and non-MLLr iB-ALL leukemias uniformly treated according to Interfant-99/06 protocol. Read More

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Selective Nonmethylated CpG DNA Recognition Mechanism of Cysteine Clamp Domains.

J Am Chem Soc 2021 May 13. Epub 2021 May 13.

Beijing Nuclear Magnetic Resonance Center, College of Chemistry and Molecular Engineering, and School of Life Sciences, Peking University, Beijing 100871, China.

Methylation of DNA at CpG sites is a major mark for epigenetic regulation, but how transcription factors are influenced by CpG methylation is not well understood. Here, we report the molecular mechanisms of how the TCF (T-cell factor) and GEF (glucose transporter 4 enhancer factor) families of proteins selectively target unmethylated DNA sequences with a C-clamp type zinc finger domain. The structure of the C-clamp domain from human GEF family protein HDBP1 (C-clamp) in complex with DNA was determined using NMR spectroscopy, which adopts a unique zinc finger fold and selectively binds RCCGG (R = A/G) DNA sequences with an "Arg···Trp-Lys-Lys" DNA recognition motif inserted in the major groove. Read More

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Can laboratory findings predict pulmonary involvement in children with COVID-19 infection?

Pediatr Pulmonol 2021 May 13. Epub 2021 May 13.

Department of Pediatric Infectious Diseases, University of Health Sciences Dr. Behçet Uz Child Disease and Pediatric Surgery Training and Research Hospital, İzmir, Turkey.

Background: Studies investigating clinical and imaging findings of coronavirus disease 2019 (COVID-19) pneumonia and predictors for lung injury mostly focus on adults. In this study, we aimed to evaluate the role of laboratory findings in predicting lung involvement in children with COVID-19.

Methods: Children with COVID-19 confirmed by reverse-transcription polymerase chain reaction or COVID-19 IgM and who underwent chest computed tomography (CT) scans were reviewed retrospectively. Read More

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The ERF-Ⅶ transcription factor SmERF73 coordinately regulates tanshinone biosynthesis in response to stress elicitors in Salvia miltiorrhiza.

New Phytol 2021 May 13. Epub 2021 May 13.

State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing, 100700, China.

Here, we investigated the role of SmERF73, a group VII ETHYLENE RESPONSE FACTOR stress response transcription factor, in the regulation of post-modification of the skeleton precursors of diterpene tanshinones in Salvia miltiorrhiza. Most genes found to be involved in tanshinone biosynthesis are located on chromosome 6, and five of which comprise a large gene cluster in S. miltiorrhiza. Read More

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Fusion Genes in Breast Cancer.

Adv Exp Med Biol 2021 ;1187:147-157

Department of Surgery, Seoul National University College of Medicine, Seoul, South Korea.

Fusion gene is a hybrid form of two distinct genes resulting in broad spectrum of downstream pathway alterations. Gene fusion events are mostly from genomic rearrangements though diverse mechanisms have now been identified from in-depth analyses of next generation sequencing data. While profound level of genomic, transcriptomic driver alterations have been identified, till now, not many gene fusions are found to be the 'driver' of cancer development nor progression. Read More

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January 2021

The p53 status in rheumatoid arthritis with focus on fibroblast-like synoviocytes.

Immunol Res 2021 May 13. Epub 2021 May 13.

Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Kargar Ave, PO-BOX: 1411713137, Tehran, Iran.

P53 is a transcription factor that regulates many signaling pathways like apoptosis, cell cycle, DNA repair, and cellular stress responses. P53 is involved in inflammatory responses through the regulation of inflammatory signaling pathways, induction of cytokines, and matrix metalloproteinase expression. Also, p53 regulates immune responses through modulating Toll-like receptors expression and innate and adaptive immune cell differentiation and maturation. Read More

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Disease-resistant identification and analysis to transcriptome differences of blueberry leaf spot induced by beta-aminobutyric acid.

Arch Microbiol 2021 May 13. Epub 2021 May 13.

Agricultural College of Guizhou University, Guiyang, 550025, China.

Leaf spot (Pestalotiopsis microspora) is one of the major fungal diseases in blueberry (Vaccinium corymbosum L.) production and if not treated promptly that can eventually lead to plant death. To prevent and control leaf spot effectively, we selected BABA (beta-aminobutyric acid) as an inducer, "Canlan" in blueberries of rabbit eyes varieties as experimental material and then induced and inoculated leaf spot on blueberries as an experimental group and used uninduced blueberries inoculated with leaf spot as the control group. Read More

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Identification of the iron-limitation stimulon in Staphylococcus lugdunensis.

Arch Microbiol 2021 May 13. Epub 2021 May 13.

EA4655 U2RM (équipe "Antibio-résistance"), CHU de Caen, Université de Caen Normandie, Caen, France.

During the infectious process, pathogens such as Staphylococcus lugdunensis have to cope with the condition of host-induced iron-limitation. Using the RNAseq approach, we performed the first global transcriptomic analysis of S. lugdunensis cells incubated in the absence and presence of iron chelator. Read More

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The role of Mig1, Mig2, Tup1 and Hap4 transcription factors in regulation of xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha.

FEMS Yeast Res 2021 May 13. Epub 2021 May 13.

Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, NAS of Ukraine, Drahomanov Street, 14/16, Lviv 79005 Ukraine.

Glucose is a preferred carbon source for most living organisms. The metabolism and regulation of glucose utilization are well studied mostly for Saccharomyces cerevisiae. Xylose is the main pentose sugar released from the lignocellulosic biomass, which has a high potential as a renewable feedstock for bioethanol production. Read More

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Combinatorial patterns of graded RhoA activation and uniform F-actin depletion promote tissue curvature.

Development 2021 May 13. Epub 2021 May 13.

Biology Department, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139, UK.

During development, gene expression regulates cell mechanics and shape to sculpt tissues. Epithelial folding proceeds through distinct cell shape changes that occur simultaneously in different regions of a tissue. Here, using quantitative imaging in Drosophila melanogaster, we investigate how patterned cell shape changes promote tissue bending during early embryogenesis. Read More

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Wedelolactone improves the renal injury induced by lipopolysaccharide in HK-2 cells by upregulation of protein tyrosine phosphatase non-receptor type 2.

J Int Med Res 2021 May;49(5):3000605211012665

Department of Critical Care Medicine, Beijing Friendship Hospital Affiliated to Capital Medical University, Beijing, China.

Objective: To explore the effects of wedelolactone (WEL) on sepsis-induced renal injury in the human renal proximal tubular epithelial cell line HK-2.

Methods: HK-2 cells were stimulated by 1 µg/ml lipopolysaccharide (LPS) to trigger renal injury . HK-2 cells were pretreated with or without WEL (0. Read More

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