767,938 results match your criteria transcription

Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease.

Stem Cell Res 2021 Apr 10;53:102321. Epub 2021 Apr 10.

Department of Biomedical Sciences, College of Medical Convergence, Catholic Kwandong University, Gangneung-si, Gangwon-do, Republic of Korea. Electronic address:

Brachyury is an embryonic nuclear transcription factor required for mesoderm formation and differentiation. Here, we introduced an mCherry reporter into the C-terminus of Brachyury in the human pluripotent stem cell line SNUhES3 using the CRISPR/Cas9 nuclease approach. Successful gene editing was verified by DNA sequencing. Read More

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Roles of liquid-liquid phase separation in bacterial RNA metabolism.

Curr Opin Microbiol 2021 Apr 17;61:91-98. Epub 2021 Apr 17.

Department of Biological Sciences, Wayne State University, Detroit, MI, 48202, USA. Electronic address:

While bacteria typically lack membrane bound organelles, the mechanisms of subcellular organization have been unclear. Bacteria have recently been found to harbor membraneless organelles containing enzymes of many biochemical pathways. These organelles, called biomolecular condensates, have been found to commonly form through the process of liquid-liquid phase separation and are typically enriched in nucleic acid binding proteins. Read More

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The FOXO signaling axis displays conjoined functions in redox homeostasis and stemness.

Free Radic Biol Med 2021 Apr 17. Epub 2021 Apr 17.

Department of Molecular, Cell and Systems Biology, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, 92521, CA, USA; Stem Cell Center, College of Natural and Agricultural Sciences, University of California Riverside, Riverside, 92521, CA, USA. Electronic address:

Previous views of reactive oxygen species (ROS) depicted them as harmful byproducts of metabolism as uncontrolled levels of ROS can lead to DNA damage and cell death. However, recent studies have shed light into the key role of ROS in the self-renewal or differentiation of the stem cell. The interplay between ROS levels, metabolism, and the downstream redox signaling pathways influence stem cell fate. Read More

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Enhancement of catabolite regulatory genes in Saccharomyces cerevisiae to increase ethanol production using hydrolysate from red seaweed Gloiopeltis furcata.

J Biotechnol 2021 Apr 17. Epub 2021 Apr 17.

Department of biotechnology, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address:

Glucose and galactose are monosaccharides obtained from Gloiopeltis furcata (Red algae). A total monosaccharide yield of 62.3 g/L was obtained from G. Read More

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ChIP-R: Assembling reproducible sets of ChIP-seq and ATAC-seq peaks from multiple replicates.

Genomics 2021 Apr 17. Epub 2021 Apr 17.

School of Chemistry and Molecular Biosciences, The University of Queensland, Cooper Road, QLD 4072, Australia; School of Chemistry and Molecular Biosciences, The University of Queensland, Cooper Road, QLD 4072, Australia. Electronic address:

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the primary protocol for detecting genome-wide DNA-protein interactions, and therefore a key tool for understanding transcriptional regulation. A number of factors, including low specificity of antibody and cellular heterogeneity of sample, may cause "peak" callers to output noise and experimental artefacts. Statistically combining multiple experimental replicates from the same condition could significantly enhance our ability to distinguish actual transcription factor binding events, even when peak caller accuracy and consistency of detection are compromised. Read More

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PcALF5 inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.

Dev Comp Immunol 2021 Apr 17:104106. Epub 2021 Apr 17.

College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, 271018, China. Electronic address:

Antimicrobial peptides (AMPs), most of which are small proteins, are necessary for innate immunity against pathogens. Anti-lipopolysaccharide factor (ALF) with a conserved lipopolysaccharide binding domain (LBD) can bind to lipopolysaccharide (LPS) and neutralize LPS activity. The antibacterial mechanism of ALF, especially its role in bacteria, needs to be further investigated. Read More

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Identification of the transcription factor, AFF4, as a new target of miR-203 in CNS.

Int J Biol Macromol 2021 Apr 17. Epub 2021 Apr 17.

The Key Laboratory of Virology of Guangzhou, Jinan University, Guangzhou, China. Electronic address:

MiR-203 was identified as a hub of a potential regulatory miRNA network in central nervous system. Overexpressing of miR-203 in the frontal cortex of C57BL/6J wild type mouse induced neurodegeneration by increasing the apoptotic pathway and neuron death. AFF4, a transcription factor, was identified as a new bona fida protein target of miR-203 in CNS. Read More

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Role of vitamin D-vitamin D receptor signaling on hyperoxia-induced bronchopulmonary dysplasia in neonatal rats.

Pediatr Pulmonol 2021 Apr 20. Epub 2021 Apr 20.

Department of Pediatrics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Background: Vitamin D exerts therapeutic effects on bronchopulmonary dysplasia (BPD), but its underlying mechanisms remain unclear. The present study was designed to investigate the effects of vitamin D on hyperoxia-induced BPD and elucidate the underlying mechanisms.

Methods: Neonatal rats were exposed to either room air (control) or 75% O (hyperoxia) and intraperitoneally injected with vitamin D3. Read More

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Enhanced triacylglycerol catabolism by Carboxylesterase 1 promotes aggressive colorectal carcinoma.

J Clin Invest 2021 Apr 20. Epub 2021 Apr 20.

Department of Immunology and Inflammation, Imperial College London, London, United Kingdom.

The ability to adapt to low-nutrient microenvironments is essential for tumor-cell survival and progression in solid cancers, such as colorectal carcinoma (CRC). Signaling by the NF-κB transcription-factor pathway associates with advanced disease stages and shorter survival in CRC patients. NF-κB has been shown to drive tumor-promoting inflammation, cancer-cell survival and intestinal epithelial cell (IEC) dedifferentiation in mouse models of CRC. Read More

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Glucose deprivation induced aberrant FUT1-mediated fucosylation drives cancer stemness in hepatocellular carcinoma.

J Clin Invest 2021 Apr 20. Epub 2021 Apr 20.

School of Biomedical Sciences, The University of Hong Kong, Hong Kong, Hong Kong.

Rapidly growing tumors often experience hypoxia and nutrient (e.g., glucose) deficiency because of poor vascularization. Read More

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Hematocrit-Related Alterations of Circulating microRNA-21 Levels in Heart Failure Patients with Reduced Ejection Fraction: A Preliminary Study.

Genet Test Mol Biomarkers 2021 Apr;25(4):302-306

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovakia.

Circulating microRNA-21 (miR-21) has been utilized as a diagnostic tool in the assessment of heart failure (HF). Blood constitution may be altered when HF occurs and miR-21 may affect hematopoiesis. Sample hemolysis may influence the determination of circulating miRNAs, challenging the diagnostic use of miRNAs. Read More

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LncRNA-HEIH is a Novel Diagnostic and Predictive Biomarker in Gastric Cancer.

Genet Test Mol Biomarkers 2021 Apr;25(4):284-292

Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Gastric cancer (GC) is associated with a high mortality rate. Long noncoding RNA (lncRNA)-high expressed in hepatocellular carcinoma (HEIH) has recently gained interest as a marker for the detection of several cancer types. This study was designed to uncover the function of lncRNA-HEIH in GC. Read More

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Betulonic Acid Derivatives Interfering with Human Coronavirus 229E Replication via the nsp15 Endoribonuclease.

J Med Chem 2021 Apr 20. Epub 2021 Apr 20.

Laboratory of Virology and Chemotherapy, Rega Institute, KU Leuven, 3000 Leuven, Belgium.

To develop antiviral therapeutics against human coronavirus (HCoV) infections, suitable coronavirus drug targets and corresponding lead molecules must be urgently identified. Here, we describe the discovery of a class of HCoV inhibitors acting on nsp15, a hexameric protein component of the viral replication-transcription complexes, endowed with immune evasion-associated endoribonuclease activity. Structure-activity relationship exploration of these 1,2,3-triazolo-fused betulonic acid derivatives yielded lead molecule as a strong inhibitor (antiviral EC: 0. Read More

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Membrane-Bound Transcriptional Activator NTL1 from Rapeseed Positively Modulates Leaf Senescence through Targeting Genes Involved in Reactive Oxygen Species Production and Programmed Cell Death.

J Agric Food Chem 2021 Apr 20. Epub 2021 Apr 20.

State Key Laboratory of Crop Stress Biology for Arid Areas and, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.

Leaf senescence is the last stage of leaf development and is determined by various environmental and endogenous signals. Leaf senescence can determine plant productivity and fitness. Transcription factors (TFs) with the transmembrane domain constitute a special group of regulatory proteins that can translocate from the membrane system into nuclei to exert the transcriptional function upon endogenous or exogenous stimuli. Read More

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The Enzymes of the Rifamycin Antibiotic Resistome.

Acc Chem Res 2021 Apr 20. Epub 2021 Apr 20.

M.G. DeGroote Institute for Infectious Disease Research, David Braley Center for Antibiotic Discovery, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8S 3Z5, Canada.

ConspectusRifamycin antibiotics include the WHO essential medicines rifampin, rifabutin, and rifapentine. These are semisynthetic derivatives of the natural product rifamycins, originally isolated from the soil bacterium . These antibiotics are primarily used to treat mycobacterial infections, including tuberculosis. Read More

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Hepatic Stellate Cell as a Mac-2-binding Protein-producing Cell in Patients with Liver Fibrosis.

Hepatol Res 2021 Apr 20. Epub 2021 Apr 20.

Department of Hepatobiliary and Pancreatic Surgery, Gunma University, Graduate School of Medicine, 3-39-22 Showamachi, Maebashi, Gunma, 371-8511, Japan.

Background: Mac-2 binding protein (M2BP) glycosylated isomer (M2BPGi) is a serum marker of liver fibrosis; M2BPGi is a glycosylated form of M2BP. Hepatocytes and hepatic stellate cells (HSCs) have each been studied to determine the source of M2BP. This study proposes to identify the origin of M2BP in fibrotic liver. Read More

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Analysis of Protein-DNA Interactions Using Surface Plasmon Resonance and a ReDCaT Chip.

Methods Mol Biol 2021 ;2263:369-379

Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.

The recognition of specific DNA sequences by proteins is crucial to fundamental biological processes such as DNA replication, transcription, and gene regulation. The technique of surface plasmon resonance (SPR) is ideally suited for the measurement of these interactions because it is quantitative, simple to implement, reproducible, can be automated, and requires very little sample. This typically involves the direct capture of biotinylated DNA to a streptavidin (SA) chip before flowing over the protein of interest and monitoring the interaction. Read More

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January 2021

Potential roles of condensin in genome organization and beyond in fission yeast.

Kyoung-Dong Kim

J Microbiol 2021 Apr 20. Epub 2021 Apr 20.

Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea.

The genome is highly organized hierarchically by the function of structural maintenance of chromosomes (SMC) complex proteins such as condensin and cohesin from bacteria to humans. Although the roles of SMC complex proteins have been well characterized, their specialized roles in nuclear processes remain unclear. Condensin and cohesin have distinct binding sites and mediate long-range and short-range genomic associations, respectively, to form cell cycle-specific genome organization. Read More

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Food intake in early life and epigenetic modifications of pro-opiomelanocortin expression in arcuate nucleus.

Mol Biol Rep 2021 Apr 20. Epub 2021 Apr 20.

Pós-Graduação em Biociência Animal, Federal University of Jataí (UFJ), Rodovia 364, Km 195, nº 3800, Jataí, Goiás, CEP 75801-615, Brasil.

The prevalence of obesity is increasing in nowadays societies and, despite being a multifactorial disease, it has a significant correlation with food intake. The control of food intake is performed by neurons of the arcuate nucleus of the hypothalamus (ARC), which secret orexigenic and anorexigenic neuropeptides, such as proopiomelanocortin (POMC), under stimulation of, e.g. Read More

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An immuno-blocking agent targeting IL-1β and IL-17A reduces the lesion of DSS-induced ulcerative colitis in mice.

Inflammation 2021 Apr 20. Epub 2021 Apr 20.

Bio-pharmaceutical Lab, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.

In recent decades when biological agents have flourished, a part of patients suffering from inflammatory bowel disease (IBD) have received the treatment of tumor necrosis factor inhibitors or IL-1 antibodies. This study aims to investigate the anti-colitis effects of bispecific antibody (FL-BsAb1/17) targeting IL-1β and IL-17A comparing with TNF-α soluble receptor medicine etanercept. IBD model in mice was established by drinking 3% DSS (dextran sulfate sodium salt). Read More

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Restored microRNA-326-5p Inhibits Neuronal Apoptosis and Attenuates Mitochondrial Damage via Suppressing STAT3 in Cerebral Ischemia/Reperfusion Injury.

Nanoscale Res Lett 2021 Apr 20;16(1):63. Epub 2021 Apr 20.

Department of Neurology, Affiliated Hospital of Yangzhou University; Yangzhou University, 45 Taizhou Road, Yangzhou, 225001, Jiangsu, People's Republic of China.

Studies have greatly explored the role of microRNAs (miRNAs) in cerebral ischemia/reperfusion injury (CI/RI). But the specific mechanism of miR-326-5p in CI/RI is still elusive. Hence, this study was to unmask the mechanism of miR-326-5p/signal transducer and activator of transcription-3 (STAT3) axis in CI/RI. Read More

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Characterization of DinJ-YafQ toxin-antitoxin module in Tetragenococcus halophilus: activity, interplay, and evolution.

Appl Microbiol Biotechnol 2021 Apr 20. Epub 2021 Apr 20.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.

Tetragenococcus halophilus is a moderately halophilic lactic acid bacterium widely used in high-salt food fermentation because of its coping ability under various stress conditions. Bacterial toxin-antitoxin (TA) modules are widely distributed and play important roles in stress response, but those specific for genus Tetragenococcus have never been explored. Here, a bona fide TA module named DinJ-YafQ was characterized in T. Read More

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[The 2017 WHO classification of pituitary tumors].

Wolfgang Saeger

Pathologe 2021 Apr 20. Epub 2021 Apr 20.

Institute für Pathologie und Neuropathologie, Universität Hamburg, Universitätsklinikum Hamburg-Eppendorf, Martinistraße 52, 20246, Hamburg, Deutschland.

The 2017 WHO classification of pituitary tumors is still based on structural analyses and expression of various pituitary hormones. Three innovations have to be considered: (1) The expression of pituitary transcription factors Pit‑1, T‑Pit and SF‑1. (2) The term "atypical adenoma" was replaced by "aggressive adenoma". Read More

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A Genetic Screen Using the Drosophila melanogaster TRiP RNAi Collection To Identify Metabolic Enzymes Required for Eye Development.

G3 (Bethesda) 2019 Jul;9(7):2061-2070

Department of Biology, Indiana University, 1001 East Third Street, Bloomington, IN 47405.

The metabolic enzymes that compose glycolysis, the citric acid cycle, and other pathways within central carbon metabolism have emerged as key regulators of animal development. These enzymes not only generate the energy and biosynthetic precursors required to support cell proliferation and differentiation, but also moonlight as regulators of transcription, translation, and signal transduction. Many of the genes associated with animal metabolism, however, have never been analyzed in a developmental context, thus highlighting how little is known about the intersection of metabolism and development. Read More

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Restriction of Cellular Plasticity of Differentiated Cells Mediated by Chromatin Modifiers, Transcription Factors and Protein Kinases.

G3 (Bethesda) 2019 Jul;9(7):2287-2302

Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY.

Ectopic expression of master regulatory transcription factors can reprogram the identity of specific cell types. The effectiveness of such induced cellular reprogramming is generally greatly reduced if the cellular substrates are fully differentiated cells. For example, in the nematode C. Read More

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Sequencing of a Wild Apple (Malus baccata) Genome Unravels the Differences Between Cultivated and Wild Apple Species Regarding Disease Resistance and Cold Tolerance.

G3 (Bethesda) 2019 Jul;9(7):2051-2060

College of Life Sciences, Northwest A&F University, Yangling 712100, Shannxi, China.

Malus baccata is one of four wild apple species that can hybridize with the cultivated apple species (Malus domestica). It is widely used in high-latitude apple-producing areas as a rootstock and breeding resource because of its disease resistance, and cold tolerance. A lack of a reference genome has limited the application of M. Read More

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RNA-Sequencing Analysis of Paternal Low-Protein Diet-Induced Gene Expression Change in Mouse Offspring Adipocytes.

G3 (Bethesda) 2019 Jul;9(7):2161-2170

RIKEN Cluster for Pioneering Research, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.

Increasing evidence indicates that parental diet affects the metabolism and health of offspring. It is reported that paternal low-protein diet (pLPD) induces glucose intolerance and the expression of genes involved in cholesterol biosynthesis in mouse offspring liver. The aim of the present study was to determine the effect of a pLPD on gene expression in offspring white adipose tissue (WAT), another important tissue for the regulation of metabolism. Read More

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Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures.

Nucleic Acids Res 2021 Apr 20. Epub 2021 Apr 20.

Division of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, USA.

Transcription elongation can be affected by numerous types of obstacles, such as nucleosome, pausing sequences, DNA lesions and non-B-form DNA structures. Spt4/5 and Elf1 are conserved transcription elongation factors that promote RNA polymerase II (Pol II) bypass of nucleosome and pausing sequences. Importantly, genetic studies have shown that Spt4/5 plays essential roles in the transcription of expanded nucleotide repeat genes associated with inherited neurological diseases. Read More

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Stomatal Development in the Context of Epidermal Tissues.

Keiko U Torii

Ann Bot 2021 Apr 20. Epub 2021 Apr 20.

Howard Hughes Medical Institute and Department of Molecular Biosciences, The University of Texas at Austin, Austin TX, USA.

Background: Stomata are adjustable pores on the surface of plant shoots for efficient gas exchange and water control. The presence of stomata is essential for plant growth and survival, and the evolution of stomata is considered as a key developmental innovation of the land plants, allowing colonization on land from aquatic environments some 450 million years ago. In the past two decades, molecular genetic studies using the model plant Arabidopsis thaliana identified key genes and signalling modules that regulate stomatal development: master-regulatory transcription factors that orchestrate cell-state transitions and peptide-receptor signal transduction pathways, which, together, enforce proper patterning of stomata within the epidermis. Read More

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Corrigendum to: TCP5 controls leaf margin development by regulating KNOX and BEL-like transcription factors in Arabidopsis.

J Exp Bot 2021 Apr 20. Epub 2021 Apr 20.

Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 518055, PR China.

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