34 results match your criteria tmem100


Tmem100-BAC-EGFP mice to selectively mark and purify embryonic endothelial cells of large caliber arteries in mid-gestational vascular formation.

Genesis 2021 Apr 2;59(4):e23416. Epub 2021 Mar 2.

Department of Molecular Physiology, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan.

Embryonic vascular development is achieved through the complex arrays of differentiation, proliferation, migration and mutual interaction of different cell types, and visualization as well as purification of unique cell populations are fundamental in studying its detailed mechanisms using in vivo experimental models. We previously demonstrated that Tmem100 was a novel endothelial gene encoding a small transmembrane protein, and that Tmem100 null mice showed embryonic lethality due to severe impairment of vascular formation. In the present study, we generated an EGFP reporter mouse line using a 216 kb genomic region containing mouse Tmem100 gene. Read More

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Tmem100- and Acta2-Lineage Cells Contribute to Implant Osseointegration in a Mouse Model.

J Bone Miner Res 2021 Feb 2. Epub 2021 Feb 2.

Hospital for Special Surgery, New York, NY, USA.

Metal implants are commonly used in orthopedic surgery. The mechanical stability and longevity of implants depend on adequate bone deposition along the implant surface. The cellular and molecular mechanisms underlying peri-implant bone formation (ie, osseointegration) are incompletely understood. Read More

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February 2021

Statistical and Functional Studies Identify Epistasis of Cardiovascular Risk Genomic Variants From Genome-Wide Association Studies.

J Am Heart Assoc 2020 04 2;9(7):e014146. Epub 2020 Apr 2.

Department of Cardiovascular and Metabolic Sciences Lerner Research Institute Cleveland Clinic Cleveland OH.

Background Epistasis describes how gene-gene interactions affect phenotypes, and could have a profound impact on human diseases such as coronary artery disease (CAD). The goal of this study was to identify gene-gene interactions in CAD using an easily generalizable multi-stage approach. Methods and Results Our forward genetic approach consists of multiple steps that combine statistical and functional approaches, and analyze information from global gene expression profiling, functional interactions, and genetic interactions to robustly identify gene-gene interactions. Read More

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TMEM100 is a key factor for specification of lymphatic endothelial progenitors.

Angiogenesis 2020 08 28;23(3):339-355. Epub 2020 Feb 28.

Department of Physiology and Functional Genomics, College of Medicine, University of Florida, 1600 SW Archer Road, Room CG-20B, Gainesville, FL, USA.

Background: TMEM100 is identified as a downstream gene of bone morphogenetic protein 9 (BMP9) signaling via activin receptor-like kinase 1 (ALK1), which is known to participate in lymphangiogenesis as well as angiogenesis. TMEM100 has been shown to be important for blood vessel formation and maintenance, but its role in the development of lymphatic vasculature remains unknown. The objective is to investigate the role of TMEM100 in development of the lymphatic system. Read More

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Identification and analysis of long non-coding RNA related miRNA sponge regulatory network in bladder urothelial carcinoma.

Cancer Cell Int 2019 3;19:327. Epub 2019 Dec 3.

1Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Chongqing, China.

Background: The aim of this study was to investigate the regulatory network of lncRNAs as competing endogenous RNAs (ceRNA) in bladder urothelial carcinoma (BUC) based on gene expression data derived from The Cancer Genome Atlas (TCGA).

Materials And Methods: RNA sequence profiles and clinical information from 414 BUC tissues and 19 non-tumor adjacent tissues were downloaded from TCGA. Differentially expressed RNAs derived from BUC and non-tumor adjacent samples were identified using the R package "edgeR". Read More

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December 2019

Transcriptome profiling-based identification of prognostic subtypes and multi-omics signatures of glioblastoma.

Sci Rep 2019 07 22;9(1):10555. Epub 2019 Jul 22.

Department of Neurosurgery, Brain Tumor Center, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

Glioblastoma (GBM) is a lethal tumor, but few biomarkers and molecular subtypes predicting prognosis are available. This study was aimed to identify prognostic subtypes and multi-omics signatures for GBM. Using oncopression and TCGA-GBM datasets, we identified 80 genes most associated with GBM prognosis using correlations between gene expression levels and overall survival of patients. Read More

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TMEM100 expression suppresses metastasis and enhances sensitivity to chemotherapy in gastric cancer.

Biol Chem 2020 02;401(2):285-296

Department of Gastrointestinal Surgery 2 Section, The First Affiliated Hospital of Fujian Medical University, No. 20 Chazhong Road, Fuzhou 350004, Fujian, China.

The gene encoding transmembrane protein 100 (TMEM100) was first discovered to be transcribed by the murine genome. It has been recently proven that TMEM100 contributes to hepatocellular carcinoma and non-small-cell lung carcinoma (NSCLC). This study investigates the impact of TMEM100 expression on gastric cancer (GC). Read More

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February 2020

Transmembrane protein 100 is expressed in neurons and glia of dorsal root ganglia and is reduced after painful nerve injury.

Pain Rep 2019 Jan-Feb;4(1):e703. Epub 2018 Dec 26.

Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA.

Introduction: Tmem100 modulates interactions between TRPA1 and TRPV1. The cell specificity of Tmem100 expression in dorsal root ganglia (DRGs) is not well defined, nor is the effect of peripheral nerve injury on Tmem100 expression.

Objective: This study was designed to determine the cell specificity of Tmem100 expression in DRG and its subcellular localization, and to examine how Tmem100 expression may be altered in painful conditions. Read More

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December 2018

Long noncoding RNA regulates endothelial cell activities associated with coronary artery disease by up-regulating , , and genes.

J Biol Chem 2019 03 17;294(11):3881-3898. Epub 2019 Jan 17.

From the Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, Ohio 44106,

Coronary artery disease (CAD) is the leading cause of death worldwide. Long noncoding RNAs (lncRNAs) are a class of noncoding transcripts of > 200 nucleotides and are increasingly recognized as playing functional roles in physiology and disease. is an lncRNA gene mapped to the chromosome 9p21 genetic locus for CAD identified by the first series of genome-wide association studies (GWAS). Read More

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TMEM100 mediates inflammatory cytokines secretion in hepatic stellate cells and its mechanism research.

Toxicol Lett 2019 Dec 11;317:82-91. Epub 2019 Jan 11.

School of Pharmacy, Anhui Key Laboratory of Bioactivity of Natural Products, Anhui Medical University, Hefei, 230032, China; Institute for Liver Diseases of Anhui Medical University, Anhui Medical University, Hefei, 230032, China. Electronic address:

Recent studies have shown that Transmembrane protein 100 (TMEM100) is a gene at locus 17q32 encoding a 134-amino acid protein with two hypothetical transmembrane domainsa, and first identified as a transcript from the mouse genome. As a downstream target gene of bone morphogenetic protein (BMP)-activin receptor-like kinase 1 (ALK1) signaling, it was activated to participate in inducing arterial endothelium differentiation, maintaining vascular integrity, promoting cell apoptosis, inhibiting metastasis and proliferation of cancer cells. However, evidence for the function of TMEM100 in inflammation is still limited. Read More

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December 2019

Time series analysis of neoadjuvant chemotherapy and bevacizumab-treated breast carcinomas reveals a systemic shift in genomic aberrations.

Genome Med 2018 11 29;10(1):92. Epub 2018 Nov 29.

Department of Genetics, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Postboks 4953 Nydalen, 0424, Oslo, Norway.

Background: Chemotherapeutic agents such as anthracyclines and taxanes are commonly used in the neoadjuvant setting. Bevacizumab is an antibody which binds to vascular endothelial growth factor A (VEGFA) and inhibits its receptor interaction, thus obstructing the formation of new blood vessels.

Methods: A phase II randomized clinical trial of 123 patients with Her2-negative breast cancer was conducted, with patients treated with neoadjuvant chemotherapy (fluorouracil (5FU)/epirubicin/cyclophosphamide (FEC) and taxane), with or without bevacizumab. Read More

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November 2018

Integrated analysis of a competing endogenous RNA network reveals key lncRNAs as potential prognostic biomarkers for human bladder cancer.

Medicine (Baltimore) 2018 Aug;97(35):e11887

Affiliated Hospital of Chengde Medical College Hebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical College, Chengde First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin China-Japan Friendship Hospital, Beijing Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Human bladder cancer (BCa) is one of the most commonly diagnosed malignancies worldwide. It has high recurrence rates and low-grade malignancy, thus representing an important public health concern. An increasing number of studies suggest that long-noncoding RNAs (lncRNAs) play important roles in various biological processes and disease pathologies, including cancer. Read More

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The expression of Transmembrane Protein 100 is regulated by alterations in calcium signaling rather than endoplasmic reticulum stress.

Biosci Biotechnol Biochem 2018 Aug 25;82(8):1377-1383. Epub 2018 Apr 25.

a Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , University of Tokyo , Tokyo , Japan.

Transmembrane protein 100 (TMEM100) comprises 134 amino acid residues and is highly conserved among vertebrates. Tmem100 has been recently reported as a key factor in angiogenesis, pain transmission, and tumor suppression. Although the importance of TMEM100 function is well supported, few studies have elucidated its expression mechanism. Read More

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BMP7 plays a critical role in TMEM100-inhibited cell proliferation and apoptosis in mouse metanephric mesenchymal cells in vitro.

In Vitro Cell Dev Biol Anim 2018 Feb 15;54(2):111-119. Epub 2017 Dec 15.

Division of Molecular Nephrology and the Creative Training Center for Undergraduates, the Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.

Kidney mainly arises from the induction of metanephric mesenchymal cells (MM cells) and the ureteric bud (UB). Transmembrane protein-100 (Tmem100) consists of two transmembrane regions with strong temporal and spatial expression characteristics during renal development. However, the function of Tmem100 in mouse embryonic kidney-derived cells remained unclear. Read More

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February 2018

Bone Morphogenetic Protein 9 Protects against Neonatal Hyperoxia-Induced Impairment of Alveolarization and Pulmonary Inflammation.

Front Physiol 2017 13;8:486. Epub 2017 Jul 13.

Division of Neonatology, Department of Pediatrics, Leiden University Medical CenterLeiden, Netherlands.

Effective treatment of premature infants with bronchopulmonary dysplasia (BPD) is lacking. We hypothesize that bone morphogenetic protein 9 (BMP9), a ligand of the TGF-β family that binds to the activin receptor-like kinase 1 (ALK1)-BMP receptor type 2 (BMPR2) receptor complex, may be a novel therapeutic option for BPD. Therefore, we investigated the cardiopulmonary effects of BMP9 in neonatal Wistar rats with hyperoxia-induced BPD. Read More

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Low-expression of TMEM100 is associated with poor prognosis in non-small-cell lung cancer.

Am J Transl Res 2017 15;9(5):2567-2578. Epub 2017 May 15.

Department of Bone Tumor Surgery, Changzheng Hospital, Second Military Medical UniversityShanghai, China.

Transmembrane protein 100 (TMEM100) was first identified as a transcript from the mouse genome. Recent studies have demonstrated that TMEM100 is involved in hepatocellular carcinoma (HCC) malignancy. However, the distribution and clinical significance of TMEM100 in non-small-cell lung carcinoma (NSCLC) remains poorly understood. Read More

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Severe damage to the placental fetal capillary network causes mid- to late fetal lethality and reduction in placental size in Peg11/Rtl1 KO mice.

Genes Cells 2017 Feb 23;22(2):174-188. Epub 2017 Jan 23.

Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.

Paternally expressed 11/Retrotransposon-like 1 (Peg11/Rtl1) knockout (KO) mice show mid- to late fetal lethality or late fetal growth retardation associated with frequent neonatal lethality. The lethal phenotype is largely dependent on genetic background and becomes more severe with each succeeding generation in the course of backcross experiments to C57BL/6 (B6). We previously suggested that these lethal and growth phenotypes in the fetal stages were due to severe defects in placental fetal capillaries in the labyrinth layer. Read More

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February 2017

Transcriptome analysis of trigeminal ganglia following masseter muscle inflammation in rats.

Mol Pain 2016 4;12. Epub 2016 Oct 4.

Department of Neural and Pain Sciences, Center to Advance Chronic Pain Research, University of Maryland Dental School, Baltimore, MD, USA.

Background: Chronic pain in masticatory muscles is a major medical problem. Although mechanisms underlying persistent pain in masticatory muscles are not fully understood, sensitization of nociceptive primary afferents following muscle inflammation or injury contributes to muscle hyperalgesia. It is well known that craniofacial muscle injury or inflammation induces regulation of multiple genes in trigeminal ganglia, which is associated with muscle hyperalgesia. Read More

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October 2017

Integrative radiogenomic analysis for genomic signatures in glioblastomas presenting leptomeningeal dissemination.

Medicine (Baltimore) 2016 Jul;95(27):e4109

Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, Korea Departments of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea Institute for Refractory Cancer Research, Samsung Medical Center, Seoul, Korea Samsung Advanced Institute of Technology, Samsung Electronics Co. Ltd., Seoul, Korea Department of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Despite therapeutic advances, the prognosis for glioblastoma (GBM) remains poor. In particular, leptomeningeal dissemination (LMD) has a dismal prognosis. The aim of this study was to identify tumor molecular phenotype, which has a great propensity to develop LMD. Read More

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Arteriovenous malformations in hereditary haemorrhagic telangiectasia: looking beyond ALK1-NOTCH interactions.

Cardiovasc Res 2016 Feb 8;109(2):196-203. Epub 2015 Dec 8.

Department of Cardiovascular Science, Centre for Molecular and Vascular Biology, KU Leuven, UZ Herestraat 49-Box 911, 3000 Leuven, Belgium

Hereditary haemorrhagic telangiectasia (HHT) is characterized by the development of arteriovenous malformations--enlarged shunts allowing arterial flow to bypass capillaries and enter directly into veins. HHT is caused by mutations in ALK1 or Endoglin; however, the majority of arteriovenous malformations are idiopathic and arise spontaneously. Idiopathic arteriovenous malformations differ from those due to loss of ALK1 in terms of both location and disease progression. Read More

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February 2016

Bioinformatics approach reveals systematic mechanism underlying lung adenocarcinoma.

Tumori 2015 May-Jun;101(3):281-6. Epub 2015 May 21.

1 Department of Respiratory Medicine, The First Hospital of Nanchang University, Nanchang, Jiangxi - PR China.

Background: The purpose of this work was to explore the systematic molecular mechanism of lung adenocarcinoma and gain a deeper insight into it.

Methods: Comprehensive bioinformatics methods were applied. Initially, significant differentially expressed genes (DEGs) were analyzed from the Affymetrix microarray data (GSE27262) deposited in the Gene Expression Omnibus (GEO). Read More

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September 2015

Novel roles of TMEM100: inhibition metastasis and proliferation of hepatocellular carcinoma.

Oncotarget 2015 Jul;6(19):17379-90

Department of Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Transmembrane protein 100 (TMEM100) was activated by ALK1/TGF-β signaling. We found that TMEM100 was decreased in hepatocellular carcinoma (HCC) tissues and in highly metastatic cell lines. Overexpressed of TMEM100 inhibited invasion, migration and proliferation. Read More

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Delineation of the clinically recognizable 17q22 contiguous gene deletion syndrome in a patient carrying the smallest microdeletion known to date.

Am J Med Genet A 2015 Sep 21;167A(9):2034-41. Epub 2015 Apr 21.

Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Madrid, Spain.

We describe a patient with a 1.34 Mb microdeletion at chromosome band 17q22, which is also present in his affected mother. To better delineate this microdeletion syndrome, we compare the clinical and molecular characteristics of 10 previously reported cases and our patient. Read More

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September 2015

Loosening pain's grip by tightening TRPV1-TRPA1 interactions.

Neuron 2015 Feb;85(4):661-3

Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road Milwaukee, WI 53226, USA. Electronic address:

TRPA1 and TRPV1 are ion channels crucial for pain sensation. In this issue of Neuron, Weng et al. (2015) demonstrate that the activity of TRPA1-TRPV1 heteromers is governed by Tmem100 and that disabling Tmem100 may be a novel pharmacologic strategy to combat pain. Read More

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February 2015

Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain.

Neuron 2015 Feb 29;85(4):833-46. Epub 2015 Jan 29.

Departments of Neuroscience and Neurosurgery, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address:

TRPA1 and TRPV1 are crucial pain mediators, but how their interaction contributes to persistent pain is unknown. Here, we identify Tmem100 as a potentiating modulator of TRPA1-V1 complexes. Tmem100 is coexpressed and forms a complex with TRPA1 and TRPV1 in DRG neurons. Read More

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February 2015

Essential role for TMEM100 in vascular integrity but limited contributions to the pathogenesis of hereditary haemorrhagic telangiectasia.

Cardiovasc Res 2015 Mar 23;105(3):353-60. Epub 2014 Dec 23.

Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Republic of Korea Department of Physiology and Functional Genomics, College of Medicine, University of Florida, 1600 SW Archer Road, Room CG-20B, Gainesville, FL 32610, USA

Aims: TMEM100 was previously identified as a downstream target of activin receptor-like kinase 1 (ALK1; ACVRL1) signalling. Mutations on ALK1 cause hereditary haemorrhagic telangiectasia (HHT), a vascular disorder characterized by mucocutaneous telangiectases and visceral arteriovenous malformations (AVMs). The aims of this study are to investigate the in vivo role of TMEM100 at various developmental and adult stages and to determine the extent to which TMEM100 contributed to the development of AVMs as a key downstream effector of ALK1. Read More

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Impairment of endothelial-mesenchymal transformation during atrioventricular cushion formation in Tmem100 null embryos.

Dev Dyn 2015 Jan 7;244(1):31-42. Epub 2014 Nov 7.

Laboratory for Cardiovascular System Research, Nara Medical University Advanced Medical Research Center, Kashihara, Nara, Japan.

Background: Endothelial-mesenchymal transformation (EndMT) is essential for endocardial cushion formation during cardiac morphogenesis. We recently identified Tmem100 as an endothelial gene indispensable for vascular development. In this study, we further investigated its roles for EndMT during atrioventricular canal (AVC) cushion formation. Read More

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January 2015

Distribution of TMEM100 in the mouse and human gastrointestinal tract--a novel marker of enteric nerves.

Neuroscience 2013 Jun 26;240:117-28. Epub 2013 Feb 26.

Enteric Neuroscience Program, Mayo Clinic, 200 1st Street SW, Rochester, MN 55905, USA.

Identification of markers of enteric neurons has contributed substantially to our understanding of the development, normal physiology, and pathology of the gut. Previously identified markers of the enteric nervous system can be used to label all or most neuronal structures or for examining individual cells by labeling just the nucleus or cell body. Most of these markers are excellent but have some limitations. Read More

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Tmem100, an ALK1 receptor signaling-dependent gene essential for arterial endothelium differentiation and vascular morphogenesis.

Proc Natl Acad Sci U S A 2012 Jul 10;109(30):12064-9. Epub 2012 Jul 10.

First Department of Internal Medicine, Nara Medical University, Kashihara, Nara, Japan.

Members of the transforming growth factor-β superfamily play essential roles in various aspects of embryonic development and physiological organ function. Among them, bone morphogenetic protein (BMP) 9 and BMP10 regulate embryonic vascular development by activating their endothelial receptor ALK1 (activin receptor-like kinase 1, also called Acvrl1). ALK1-mediated intracellular signaling is implicated in the etiologies of human diseases, but their downstream functional proteins are largely unknown. Read More

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