914 results match your criteria tma test


High Gene Expression Is Associated With Poor Prognosis in Glioma Patients.

Front Cell Dev Biol 2021 17;9:679684. Epub 2021 Jun 17.

Huaxi MR Research Center, Department of Radiology, Functional and Molecular Imaging Key Laboratory of Sichuan Province, West China Hospital, Sichuan University, Chengdu, China.

Objective: Immediate early response 5 () plays a core role in cell cycle and response to irradiation. However, its role in glioma remains unclear. We aimed to evaluate its prognostic significance in glioma based on The Cancer Genome Atlas data resource. Read More

View Article and Full-Text PDF

Thrombotic microangiopathy increases the risk of chronic kidney disease but not overall mortality in long-term transplant survivors.

Transplant Cell Ther 2021 Jul 1. Epub 2021 Jul 1.

Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA; Division of Nephrology, Seattle Children's Hospital, Seattle, WA; Department of Pediatrics, University of Washington School of Medicine, Seattle, WA.

Background: Thrombotic microangiopathy (TMA) after allogeneic hematopoietic cell transplant (HCT) is associated with acute kidney injury (AKI) and increased mortality. The impact of TMA on chronic kidney disease (CKD) and long-term mortality among HCT survivors has not been fully examined.

Objectives: To assess the risk of CKD and mortality in HCT survivors with and without history of TMA. Read More

View Article and Full-Text PDF

Unraveling the mechanism of octenidine and chlorhexidine on membranes: does electrostatics matter?

Biophys J 2021 Jun 29. Epub 2021 Jun 29.

Wroclaw University of Science and Technology, Faculty of Fundamental Problems of Technology, Department of Biomedical Engineering, Wroclaw, Poland.

The increasing problem of antibiotic resistance in bacteria requires the development of new antimicrobial candidates. There are several well-known substances with commercial use, but their molecular mode of action is not fully understood. In this paper, we focus on two commonly used antimicrobial agents from the detergent family - octenidine dichloride (OCT) and chlorhexidine digluconate (CHX). Read More

View Article and Full-Text PDF

The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time.

J Clin Lab Anal 2021 Jul 2:e23888. Epub 2021 Jul 2.

Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, USA.

Background: The Hologic Aptima™ TMA SARS-CoV-2 assay was employed to test pooled nasopharyngeal (NP) samples to evaluate the performance of pooled sample testing and characterize variables influencing results.

Methods: Results on 1033 previously tested NP samples were retrieved to characterize the relative light units (RLU) of SARS-CoV-2-positive samples in the tested population. The pooling strategy of combining 10 SARS-CoV-2 samples into one pool (10/1) was used in this study. Read More

View Article and Full-Text PDF

TIMAP Upregulation Correlates Negatively with Survival in HER2- Negative Subtypes of Breast Cancer.

Asian Pac J Cancer Prev 2021 Jun 1;22(6):1899-1905. Epub 2021 Jun 1.

Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.

Objective: TIMAP expression is regulated by transforming growth factor beta 1 (TGFβ1); known for its role in breast cancer development and metastasis. Nevertheless, data on TIMAP protein expression and its association with breast cancer development are lacking. In this study, we aimed to investigate the variation in TIMAP protein expression in breast cancer tissue and its correlation with various clinicopathological characteristics of breast cancer patients and overall survival rate. Read More

View Article and Full-Text PDF

Preliminary Validation of the Triana Test: A New Story Recall Test Based on Emotional Material.

Am J Alzheimers Dis Other Demen 2021 Jan-Dec;36:15333175211025911

Memory Unit, 16885Virgen del Rocio University Hospital, Seville, Spain.

Objective: To first validate the diagnostic accuracy of the "Triana Test," a new story recall test based on emotional material.

Method: A phase I study of validation. We included 55 patients with amnestic Mild Cognitive Impairment and 69 healthy controls, diagnosed according to the "Memory Associative Test of the district of Seine-Saint-Denis" (TMA-93), and matched by age, gender, and educational level. Read More

View Article and Full-Text PDF

Expression patterns for Bcl-2, EMA, β-catenin, E-cadherin, PAX8, and MIB1 in thymomas.

Mod Pathol 2021 Jun 16. Epub 2021 Jun 16.

Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.

The expression of immunohistochemical markers has been extensively investigated in thymomas to assist in the differential diagnosis. We have studied six select markers to determine their utility in the evaluation of these tumors. A series of 126 thymomas including 33 type A, 27 type AB, 20 type B1, 22 type B2, and 24 type B3, were examined utilizing a tissue microarray (TMA) technique with antibodies to e-cadherin, β-catenin, PAX8, bcl-2, EMA, and MIB-1. Read More

View Article and Full-Text PDF

Mechanical force system of double key loop with finite element analysis.

BMC Oral Health 2021 06 13;21(1):301. Epub 2021 Jun 13.

Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Background: The mechanics of double key loop (DKL) are not well defined, and this finite element study was designed to explore its force system.

Methods: A simplified 3-dimensional finite element model of single and double key loops with an archwire between the lateral incisor and second premolar was established in Ansys Workbench 17.0. Read More

View Article and Full-Text PDF

[Thrombotic thrombocytopenic purpura].

Rinsho Ketsueki 2021 ;62(5):430-434

Saitama Medical University Hospital.

In this article, I mainly review the molecular targeted therapy for thrombotic thrombocytopenic purpura (TTP). TTP is one of thrombotic microangiopathies (TMA), which demonstrate hemolytic anemia with red blood cell destruction and thrombocytopenia. Another TTP, hemolytic uremic anemia (HUS) reveals bloody stood and acute kidney failure. Read More

View Article and Full-Text PDF

Comparison of surface topography of low-friction and conventional TMA orthodontic arch wires using atomic force microscopy.

J Orthod Sci 2021 19;10. Epub 2021 Feb 19.

Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

Objective: To evaluate the surface topography and roughness of orthodontic arch wire materials, including low-friction titanium molybdenum alloy (TMA), conventional TMA, and stainless-steel arch wires.

Materials And Methods: The surface topography was evaluated using atomic force microscopy (AFM). A total of 24 wire specimens were used for the AFM scans {8 low-friction TMA (TMA-Low), 8 conventional TMA (TMA-C), and 8 stainless steel (SS)} (Ormco, Orange, CA, USA), measuring 0. Read More

View Article and Full-Text PDF
February 2021

Three-dimensional soft tissue changes in orthodontic extraction and non-extraction patients: A prospective study.

Orthod Craniofac Res 2021 Jun 3. Epub 2021 Jun 3.

Department of Dentistry and Oral Health, Section of Orthodontics, Aarhus University, Aarhus, Denmark.

Objectives: To assess the soft tissue changes in orthodontic extraction and non-extraction patients on 3D stereophotogrammetric images.

Setting And Sample: 23 extraction (22.2 ± 9. Read More

View Article and Full-Text PDF

Association Between Alkaline Phosphatase and Muscle Mass, Strength, or Physical Performance in Patients on Maintenance Hemodialysis.

Front Med (Lausanne) 2021 17;8:657957. Epub 2021 May 17.

Division of Nephrology, Department of Internal Medicine, CHA Gumi Medical Center, CHA University, Gumi, South Korea.

Alkaline phosphatase (ALP) is an indicator for checking liver or bone disorders, but recent studies have shown the possibility of an additive indicator beyond the simple mineral-bone status in dialysis patients. The aim of the study was to evaluate the ALP level and various indicators for malnutrition, physical performance, or hospitalization in patients on hemodialysis (HD). This study was an observational study ( = 84). Read More

View Article and Full-Text PDF

Change of tibial tuberosity-trochlear groove (TT-TG) distance during total knee arthroplasty had no influence on clinical outcome and anterior knee pain.

Int Orthop 2021 Jun 1. Epub 2021 Jun 1.

Department of Orthopaedic Surgery and Traumatology, Kantonsspital Baselland (Bruderholz, Liestal, Laufen), CH-4101, Bruderholz, Switzerland.

Purpose: The main purpose of this study was to determine whether there is a correlation between the change of tibial tuberosity-trochlear groove (TT-TG) distance and clinical outcomes after total knee arthroplasty (TKA).

Methods: A total of 52 knees undergoing TKA due to primary osteoarthritis were included in this retrospective study. All patients had pre- and postoperative CT scans. Read More

View Article and Full-Text PDF

Diagnosing SARS-CoV-2 with Antigen Testing, Transcription-Mediated Amplification and Real-Time PCR.

J Clin Med 2021 May 29;10(11). Epub 2021 May 29.

Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany.

This study was performed as a head-to-head comparison of the performance characteristics of (1) two SARS-CoV-2-specific rapid antigen assays with real-time PCR as gold standard as well as (2) a fully automated high-throughput transcription-mediated amplification (TMA) assay and real-time PCR in a latent class analysis-based test comparison without a gold standard with several hundred samples in a low prevalence "real world" setting. Recorded sensitivity and specificity of the NADAL and the LumiraDx antigen assays and the Hologic Aptima SARS-CoV-2 TMA assay were 0.1429 (0. Read More

View Article and Full-Text PDF

MET Expression and Cancer Stem Cell Networks Impact Outcome in High-Grade Serous Ovarian Cancer.

Genes (Basel) 2021 May 14;12(5). Epub 2021 May 14.

Division of Oncology, Department of Clinical Sciences Lund, Lund University and Skåne University Hospital, 223 81 Lund, Sweden.

Overexpression of the receptor tyrosine kinase MET has been linked to poor survival in several cancer types, and MET has been suggested to interact with stem cell networks. In vitro studies have further suggested a possible benefit of a combined treatment using PARP and MET inhibitors. We used a tissue microarray (TMA) with 130 samples of advanced-stage high-grade serous fallopian tube/ovarian cancer (HGSC) to investigate the prognostic value of MET protein expression alone and in combination with the stem cell factor SOX2. Read More

View Article and Full-Text PDF

Same-day SARS-CoV-2 antigen test screening in an indoor mass-gathering live music event: a randomised controlled trial.

Lancet Infect Dis 2021 May 27. Epub 2021 May 27.

Division of Infectious diseases and Foundation for Fighting AIDS, Infectious Diseases and Promoting Health and Science, University Hospital Germans Trias i Pujol, Badalona, Spain. Electronic address:

Background: The banning of mass-gathering indoor events to prevent SARS-CoV-2 spread has had an important effect on local economies. Despite growing evidence on the suitability of antigen-detecting rapid diagnostic tests (Ag-RDT) for mass screening at the event entry, this strategy has not been assessed under controlled conditions. We aimed to assess the effectiveness of a prevention strategy during a live indoor concert. Read More

View Article and Full-Text PDF

Minipool testing for SARS-CoV-2 RNA in United States blood donors.

Transfusion 2021 May 27. Epub 2021 May 27.

Vitalant Research Institute, San Francisco, California, USA.

Background: SARS-CoV-2 RNA prevalence in blood donors from large geographic areas of high community transmission is limited. We tested residual donor plasma minipools (MPs) to determine SARS-CoV-2 RNAemia prevalence in six United States areas.

Study Design/methods: Blood donations collected from 7 March 2020 to 25 September 2020 were tested for SARS-CoV-2 RNA (vRNA) in MP of 6 or 16 donations using the Grifols Procleix SARS-CoV-2 research-use only (RUO) transcription-mediated amplification (TMA) assay. Read More

View Article and Full-Text PDF

TMA-93 Validation by Alzheimer's Disease Biomarkers: A Comparison with the Free and Cued Selective Reminding Test on a Biobank Sample.

J Alzheimers Dis 2021 ;82(1):401-410

Memory Unit, Virgen del Rocio University Hospital, Seville, Spain.

Background: The Memory Associative Test TMA-93 examines visual relational binding, characteristically affected in early-AD stages.

Objective: We aim to validate the TMA-93 by biomarkers determination and compare its diagnostic characteristics with the Free and Cued Selective Reminding Test (FCSRT).

Methods: Retrospective analysis of a Biobank database. Read More

View Article and Full-Text PDF
January 2021

Clinical Characteristics and Prognosis of Renal Thrombotic Microangiopathy in Lupus Nephritis.

Iran J Kidney Dis 2021 May;15(3):169-176

Department of Nephrology, the First Affiliated Hospital of Zhengzhou University, Institute of Nephrology, Zhengzhou University, Zhengzhou City, Henan Province, China.

Introduction: LN is an important complication affecting the prognosis of SLE. We retrospectively analysed the influence of thrombotic microangiopathy (TMA) on LN, identified risk factors of TMA in LN and renal failure in LN-TMA, and evaluated the availability of plasmapheresis.

Methods: After balancing epidemiological characteristics and pathological types between groups, 127 patients (LN-TMA:42, LN:85) were included. Read More

View Article and Full-Text PDF

Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay.

J Virol Methods 2021 08 10;294:114182. Epub 2021 May 10.

Institute for Medical Virology, University Hospital Tuebingen, Elfriede-Aulhorn-Str. 6, 72076 Tuebingen, Germany. Electronic address:

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic requires fast and accurate high-throughput diagnostic tools. To evaluate the analytical performance of the Hologic Aptima transcription-mediated amplification (TMA) assay for detection of SARS-CoV-2 RNA from respiratory samples we analysed 103 clinical and proficiency panel samples pre-tested by real-time RT-PCR (Altona, RealStar) and found a positive percent agreement (sensitivity) of 95.7 % and a negative percent agreement (specificity) of 100 %. Read More

View Article and Full-Text PDF

Endogenous Retroviral-K Envelope Is a Novel Tumor Antigen and Prognostic Indicator of Renal Cell Carcinoma.

Front Oncol 2021 22;11:657187. Epub 2021 Apr 22.

Department of Gynecology and Obstetrics, University Hospital Erlangen, Comprehensive Cancer Center, European Metropolitan Area Erlangen-Nuremberg (CCC ER-EMN), Friedrich-Alexander-University Erlangen-Nuernberg, Erlangen, Germany.

Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. Read More

View Article and Full-Text PDF

BRCA1 Protein Expression Predicts Survival in Glioblastoma Patients from an NRG Oncology RTOG Cohort.

Oncology 2021 May 6:1-9. Epub 2021 May 6.

Department of Radiation Oncology, Weill Cornell Medicine, New York, New York, USA.

Purpose: Glioblastoma, the most common malignant brain tumor, was associated with a median survival of <1 year in the pre-temozolomide (TMZ) era. Despite advances in molecular and genetic profiling studies identifying several predictive biomarkers, none has been translated into routine clinical use. Our aim was to investigate the prognostic significance of a panel of diverse cellular molecular markers of tumor formation and growth in an annotated glioblastoma tissue microarray (TMA). Read More

View Article and Full-Text PDF

High TIL, HLA, and Immune Checkpoint Expression in Conventional High-Grade and Dedifferentiated Chondrosarcoma and Poor Clinical Course of the Disease.

Front Oncol 2021 12;11:598001. Epub 2021 Apr 12.

Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States.

Purpose: The aim of this study was to characterize chondrosarcoma tumor infiltration by immune cells and the expression of immunologically relevant molecules. This information may contribute to our understanding of the role of immunological events in the pathogenesis of chondrosarcoma and to the rational design of immunotherapeutic strategies.

Patients And Methods: A tissue microarray (TMA) containing 52 conventional and 24 dedifferentiated chondrosarcoma specimens was analyzed by immunohistochemical staining for the expression of parameters associated with tumor antigen-specific immune responses, namely, CD4 and CD8 tumor infiltrating lymphocytes (TILs) and the expression of HLA class I heavy chain, beta-2 microglobulin (β2m), HLA class II and immune checkpoint molecules, B7-H3 and PD-1/PD-L1. Read More

View Article and Full-Text PDF

Development of a Novel Weighted Ranking Method for Immunohistochemical Quantification of a Heterogeneously Expressed Protein in Gastro-Esophageal Cancers.

Cancers (Basel) 2021 Mar 13;13(6). Epub 2021 Mar 13.

Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland.

High expression of Junctional Adhesion Molecule-A (JAM-A) has been linked with poor prognosis in several cancers, including breast cancers overexpressing the human epidermal growth factor receptor-2 (HER2). Furthermore, JAM-A expression has been linked with regulating that of HER2, and associated with the development of resistance to HER2-targeted therapies in breast cancer patients. The purpose of this study was to establish a potential relationship between JAM-A and HER2 in HER2-overexpressing gastro-esophageal (GE) cancers. Read More

View Article and Full-Text PDF

Interventions for atypical haemolytic uraemic syndrome.

Cochrane Database Syst Rev 2021 Mar 23;3:CD012862. Epub 2021 Mar 23.

Renal Department, Newcastle University/Freeman Hospital, Newcastle Upon Tyne, UK.

Background: Atypical haemolytic uraemic syndrome (aHUS) is a rare disorder characterised by thrombocytopenia, microangiopathic haemolytic anaemia, and acute kidney injury. The condition is primarily caused by inherited or acquired dysregulation of complement regulatory proteins with ~40% of those affected aged < 18 years. Historically, kidney failure and death were common outcomes, however, improved understanding of the condition has led to discovery of novel therapies. Read More

View Article and Full-Text PDF

Simple method for constructing and repairing tissue microarrays using simple equipment.

J Int Med Res 2021 Mar;49(3):3000605211000156

Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Objective: Many methods for tissue microarray (TMA) construction were described in previous reports. Because TMA-based methods are expensive and complicated, their widespread application may be restricted. This study aimed to develop a simple method for TMA construction. Read More

View Article and Full-Text PDF

Higher Acid Recovery Efficiency of Novel Functionalized Inorganic/Organic Composite Anion Exchange Membranes from Acidic Wastewater.

Membranes (Basel) 2021 Feb 14;11(2). Epub 2021 Feb 14.

Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.

In this work, the synthesis of a series of the functionalized inorganic/organic composite anion exchange membranes (AEMs) was carried out by employing the varying amount of inorganic filler consist of -(trimethoxysilylpropyl)-,,-trimethylammonium chloride (TMSP-TMACl) into the quaternized poly (2, 6-dimethyl-1, 4-phenylene oxide) (QPPO) matrix for acid recovery via diffusion dialysis (DD) process. Fourier transform infrared (FTIR) spectroscopy clearly demonstrated the fabrication of the functionalized inorganic/organic composite AEMs and the subsequent membrane characteristic measurements such as ion exchange capacity (IEC), linear swelling ratio (LSR), and water uptake (W) gave us the optimum loading condition of the filler without undesirable filler particle aggregation. These composite AEMs exhibited IEC of 2. Read More

View Article and Full-Text PDF
February 2021

Clinical Significance of Receptor-Interacting Protein 3 and Parkin, Essential Molecules for Necroptosis, in Breast Cancer.

J Breast Cancer 2021 Feb;24(1):34-48

Department of Surgery, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, Korea.

Purpose: Receptor-interacting protein 3 (RIP3) is the main initiator of necroptosis. Parkin prevents the formation of the RIP1-RIP3 complex by promoting polyubiquitination of RIP3. However, the mechanism by which necroptosis affects the clinical features of breast cancer and prognosis is not known. Read More

View Article and Full-Text PDF
February 2021

Comparison of multiple flatfoot indicators in 5-8-year-old children.

Open Med (Wars) 2021 1;16(1):246-256. Epub 2021 Feb 1.

Department of Paediatric Surgery Pediatric Orthopedics - Traumatology Unit, Lithuanian University of Health Sciences, A. Mickevičiaus g. 9, LT-44307, Kaunas, Lithuania.

Background: The foot posture is age dependent. The purpose of this study was to investigate the relationship between the 6-item version of the foot posture index (FPI) and other clinical, foot anthropometric, radiological measurements for the foot position in 5-8-year-old children.

Methods: A total of 301 participants with a mean age of 6. Read More

View Article and Full-Text PDF
February 2021

Schistocyte quantitation, thrombotic microangiopathy and acute kidney injury in Australian snakebite coagulopathy [ASP28].

Int J Lab Hematol 2021 Feb 22. Epub 2021 Feb 22.

Clinical Toxicology Research Group, University of Newcastle, Newcastle, NSW, Australia.

Introduction: The major systemic manifestation of hemotoxicity in human snakebite envenoming is venom-induced consumption coagulopathy (VICC). A subset of patients with VICC develop thrombotic microangiopathy (TMA), in which acute kidney injury (AKI) occurs. We aimed to investigate the association between schistocytosis in snakebite patients with VICC and AKI, compared to non-envenomed patients. Read More

View Article and Full-Text PDF
February 2021