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Implementing "chest pain pathway" using smartphone messaging application "WhatsApp" as a corrective action plan to improve ischemia time in "ST-Elevation myocardial Infarction" in primary PCI capable center "WhatsApp-STEMI Trial".

Wesam A AlHejily

Crit Pathw Cardiol 2021 Jul 27. Epub 2021 Jul 27.

Department of Medicine (Division of Cardiology) Faculty of Medicine, King Abdelaziz University Hospital, Jeddah, Saudi Arabia Department of Cardiology division at Dr Sulaiman Alhabib medical group.

Background: ST-elevation myocardial infarction (STEMI) is a life-threatening medical emergency that requires immediate medical attention, each hospital should implement a clinical pathway with a main objective to reduce ischemia time from diagnosis to revascularization, as this has shown to save myocardial tissues and subsequently patients' lives, utilizing the most evidence-based approach and the most up to date management protocol. In this study we aimed to assess the utility of structuring chest pain pathway with instantaneous case by case feedback protocol using WhatsApp as a smartphones' messaging application and its impact on improving two major KPIs, the impact on initial ER door to ECG time, and door to balloon time, in a predefined 6-month corrective action period.

Methods: Prospective, quality improvement plan was set to reduce door to first ECG and door to balloon times to less than 10 and 90 minutes, respectively in all ACS cases including STEMI, from August 2020 to April 30th, 2021. Read More

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Bioaccumulation of current-use herbicides in fish from a global biodiversity hotspot: Lake St Lucia, South Africa.

Chemosphere 2021 Jul 2;284:131407. Epub 2021 Jul 2.

School of Chemistry, University of the Witwatersrand, Johannesburg, Private Bag 3, WITS, 2050, South Africa. Electronic address:

Agricultural pesticides threaten aquatic systems and biodiversity at a global scale, but limited information is available on the accumulation of current-use herbicides in tissues of aquatic organisms. Here, we examine the potential exposure and accumulation of currently used herbicides in two species of fish from Lake St Lucia, a global biodiversity hotspot located in South Africa. Muscle tissue samples were analysed for 11 widely used multi-residue and phenoxy-acid herbicides. Read More

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Zeolite-supported manganese oxides decrease the Cd uptake of wheat plants in Cd-contaminated weakly alkaline arable soils.

J Hazard Mater 2021 Jun 24;419:126464. Epub 2021 Jun 24.

Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

Cd pollution in arable soils has posed serious threats to food safety and human health. Mn oxides and Mn oxide-based materials have been widely applied to the removal of heavy metals for their high adsorption capacity, especially in water treatment. However, the performance and stability of Mn oxide-based materials and the underlying mechanism of Cd immobilization in upland soils remain unclear. Read More

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Gaseous ozone to preserve quality and enhance microbial safety of fresh produce: Recent developments and research needs.

Xuetong Fan

Compr Rev Food Sci Food Saf 2021 Jul 29. Epub 2021 Jul 29.

Eastern Regional Research Center, U.S. Department of Agriculture, Agricultural Research Service, Wyndmoor, Pennsylvania, USA.

Fresh fruits and vegetables are highly perishable and are subject to large postharvest losses due to physiological (senescence), pathologic (decay), and physical (mechanical damage) factors. In addition, contamination of fresh produce with foodborne human pathogens has become a concern. Gaseous ozone has multiple benefits including destruction of ethylene, inactivation of foodborne and spoilage microorganisms, and degradation of chemical residues. Read More

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GNAI2 exacerbates non-alcoholic steatohepatitis progression via regulating PRDX1-related inflammation and lipophagy.

Hepatology 2021 Jul 29. Epub 2021 Jul 29.

Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

Non-alcoholic steatohepatitis (NASH) is an advanced stage of liver disease accompanied by lipid accumulation, inflammation, and liver fibrosis. Guanine nucleotide-binding protein G(i) subunit alpha-2 (GNAI2) is a member of the "inhibitory" class of α-subunits and resent studies showed Gnai2-deficiency is known to cause reduced weight in mice. However, the role of GNAI2 in hepatocytes, particularly in the context of liver inflammation and lipid metabolism, remains to be elucidated. Read More

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Collective Cell Migration in a Fibrous Environment: A Hybrid Multiscale Modelling Approach.

Front Appl Math Stat 2021 Jun 25;7. Epub 2021 Jun 25.

Department of Mathematics, University of Dundee, Dundee, UK.

The specific structure of the extracellular matrix (ECM), and in particular the density and orientation of collagen fibres, plays an important role in the evolution of solid cancers. While many experimental studies discussed the role of ECM in individual and collective cell migration, there are still unanswered questions about the impact of nonlocal cell sensing of other cells on the overall shape of tumour aggregation and its migration type. There are also unanswered questions about the migration and spread of tumour that arises at the boundary between different tissues with different collagen fibre orientations. Read More

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The Impacts of Surgery and Intracerebral Electrodes in C57BL/6J Mouse Kainate Model of Epileptogenesis: Seizure Threshold, Proteomics, and Cytokine Profiles.

Front Neurol 2021 12;12:625017. Epub 2021 Jul 12.

Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, United Kingdom.

Intracranial electroencephalography (EEG) is commonly used to study epileptogenesis and epilepsy in experimental models. Chronic gliosis and neurodegeneration at the injury site are known to be associated with surgically implanted electrodes in both humans and experimental models. Currently, however, there are no reports on the impact of intracerebral electrodes on proteins in the hippocampus and proinflammatory cytokines in the cerebral cortex and plasma in experimental models. Read More

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Protean role of epigenetic mechanisms and their impact in regulating the Tregs in TME.

Cancer Gene Ther 2021 Jul 28. Epub 2021 Jul 28.

Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, 600 077, India.

Constitutive expression of Foxp3+ Tregs in the tumor microenvironment (TME) specifically renders immune suppression in the tumor tissues. Being highly stable and self-tolerant, Tregs may be influenced by various epigenetic-associated mechanisms while exhibiting their functions. DNA methylation, histone acetylation, epigenetic silencing, alteration in chromatin networks, etc. Read More

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4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis.

Prostate 2021 Jul 28. Epub 2021 Jul 28.

Department of Urology, The First Affiliated Hospital of Anhui Medical University, Institute of Urology, and Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China.

Background: Hyaluronan (HA), an extracellular matrix component, accumulates in most chronic inflammatory tissues. Here, we studied the impact of HA on the pathogenesis of chronic prostatitis.

Materials And Methods: First, we sorted demographic characteristics and peripheral blood serum samples from patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) to assess the relationship between the levels of HA in peripheral blood serum and the severity of inflammation in patients. Read More

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Loss of the mitochondrial phosphate carrier SLC25A3 induces remodeling of the cardiac mitochondrial protein acylome.

Am J Physiol Cell Physiol 2021 07 28. Epub 2021 Jul 28.

Division of Pediatric Cardiology, Department of Pediatrics, Emory University School of Medicine, and Children's Healthcare of Atlanta, Atlanta, GA, United States.

Mitochondria are recognized as signaling organelles because, under stress, mitochondria can trigger various signaling pathways to coordinate the cell's response. The specific pathway(s) engaged by mitochondria in response to mitochondrial energy defects in vivo and in high-energy tissues like the heart are not fully understood. Here, we investigated cardiac pathways activated in response to mitochondrial energy dysfunction by studying mice with cardiomyocyte-specific loss of the mitochondrial phosphate carrier (SLC25A3), an established model that develops cardiomyopathy as a result of defective mitochondrial ATP synthesis. Read More

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Loss of crossbridge inhibition drives pathological cardiac hypertrophy in patients harboring the TPM1 E192K mutation.

J Gen Physiol 2021 Sep 28;153(9). Epub 2021 Jul 28.

Department of Biomedical Engineering, Yale University, New Haven, CT.

Hypertrophic cardiomyopathy (HCM) is an inherited disorder caused primarily by mutations to thick and thinfilament proteins. Although thin filament mutations are less prevalent than their oft-studied thick filament counterparts, they are frequently associated with severe patient phenotypes and can offer important insight into fundamental disease mechanisms. We have performed a detailed study of tropomyosin (TPM1) E192K, a variant of uncertain significance associated with HCM. Read More

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September 2021

A chondroitin sulfate proteoglycan 4‑specific monoclonal antibody inhibits melanoma cell invasion in a spheroid model.

Int J Oncol 2021 Sep 28;59(3). Epub 2021 Jul 28.

Department of Dermatology, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, A-3100 St. Poelten, Austria.

The overexpression of chondroitin sulfate proteoglycan 4 (CSPG4) is associated with several tumor types, including malignant melanoma, squamous cell carcinoma, triple‑negative breast carcinoma, oligodendrocytomas or gliomas. Due to its restricted distribution in normal tissues, CSPG4 has been considered a potential target for several antitumor approaches, including monoclonal antibody (mAb) therapies. The aim of the present study was to characterize the impact of the CSPG4‑specific mAb clone 9. Read More

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September 2021

lncRNA-PACER upregulates and PGE2 through the NF-κB pathway to promote the proliferation and invasion of colorectal-cancer cells.

Gastroenterol Rep (Oxf) 2021 Jun 10;9(3):257-268. Epub 2020 Dec 10.

Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P. R. China.

Background: p50-associated cyclooxygenase-2 extragenic RNA (PACER) is a recently identified antisense long non-coding RNA (lncRNA) located on the upstream of the promoter region of cyclooxygenase-2 (). Preliminary studies have suggested that PACER is involved in the regulation of expression in macrophagocyte and osteosarcoma cells. However, the role of this lncRNA in colorectal cancer (CRC) remains elusive. Read More

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Shotgun lipidomics of liver and brain tissue of Alzheimer's disease model mice treated with acitretin.

Sci Rep 2021 Jul 27;11(1):15301. Epub 2021 Jul 27.

Experimental Neurology, Saarland University, Homburg, Saar, Germany.

Alzheimer's disease (AD) is a very frequent neurodegenerative disorder characterized by an accumulation of amyloid-β (Aβ). Acitretin, a retinoid-derivative and approved treatment for Psoriasis vulgaris, increases non-amyloidogenic Amyloid-Precursor-Protein-(APP)-processing, prevents Aβ-production and elicits cognitive improvement in AD mouse models. As an unintended side effect, acitretin could result in hyperlipidemia. Read More

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IGHG1 upregulation promoted gastric cancer malignancy via AKT/GSK-3β/β-Catenin pathway.

Cancer Cell Int 2021 Jul 27;21(1):397. Epub 2021 Jul 27.

Department of Gastrointestinal Surgery, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou City, 362002, Fujian Province, China.

Background: Despite current advances in gastric cancer treatment, disease metastasis and chemo-resistance remain as major hurdles against better overall prognosis. Previous studies indicated that IGHG1 as well as -Catenin serve as important regulators of tumor cellular malignancy. Therefore, understanding detailed molecular mechanism and identifying druggable target will be of great potentials in future therapeutic development. Read More

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Anti-F4/80 treatment attenuates Th2 cell responses: Implications for the role of lung interstitial macrophages in the asthmatic mice.

Int Immunopharmacol 2021 Jul 24;99:108009. Epub 2021 Jul 24.

Department of Respiratory & Critical Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China. Electronic address:

Lung interstitial macrophages (IMs) can be polarized towards an alternative activation phenotype in ovalbumin (OVA)-induced asthmatic mice. However, the role of alternative activation of lung IMs in Th2 cell responses in the asthmatic murine is still unclear. Here, we leverage an anti-F4/80 treatment which has been shown to selectively deplete IMs in mice and investigate how this treatment modulates Th2 cell responses in lung and whether the modulation is dependent on lung IMs in murine models of asthma. Read More

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The impact of artificial selection for Wolbachia-mediated dengue virus blocking on phage WO.

PLoS Negl Trop Dis 2021 Jul 27;15(7):e0009637. Epub 2021 Jul 27.

Department of Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America.

Wolbachia is currently at the forefront of global efforts to control arbovirus transmission from the vector Aedes aegypti. The use of Wolbachia relies on two phenotypes-cytoplasmic incompatibility (CI), conferred by cifA and cifB genes in prophage WO, and Wolbachia-mediated pathogen blocking (WMPB). These traits allow for local, self-sustaining reductions in transmission of dengue (DENV) following release of Wolbachia-infected A. Read More

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Neuropathological effects of chronically implanted, intracortical microelectrodes in a tetraplegic patient.

J Neural Eng 2021 Jul 27;18(4). Epub 2021 Jul 27.

Department of Pathology, Keck USC School of Medicine, Los Angeles, CA, United States of America.

Intracortical microelectrode arrays (MEA) can be used as part of a brain-machine interface system to provide sensory feedback control of an artificial limb to assist persons with tetraplegia. Variability in functionality of electrodes has been reported but few studies in humans have examined the impact of chronic brain tissue responses revealed postmortem on electrode performanceIn a tetraplegic man, recording MEAs were implanted into the anterior intraparietal area and Brodmann's area 5 (BA5) of the posterior parietal cortex and a recording and stimulation array was implanted in BA1 of the primary somatosensory cortex (S1). The participant expired from unrelated causes seven months after MEA implantation. Read More

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J Orthop Res 2021 Jul 27. Epub 2021 Jul 27.

Department of Orthopaedic Surgery, Stanford University, 240 Pasteur Drive, Stanford, CA, 94304, USA.

In the induced membrane (IM) technique for bone reconstruction, a poly(methyl methacrylate) (PMMA) spacer is implanted to induce formation of a foreign body membrane around the defect site. Membrane development is essential for later bone grafting success, yet the mechanism by which the IM promotes bone regeneration remains unknown, as are the ways that spacer composition plays a role in the membrane's healing potential. This study investigated the impact of leached methyl methacrylate (MMA) - the major monomeric component of PMMA - on IM development. Read More

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Occurrence, functionality, and abundance of the TERT promoter mutations.

Int J Cancer 2021 Jul 27. Epub 2021 Jul 27.

Division of Functional Genome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Telomere shortening at chromosomal ends due to the constraints of the DNA replication process acts as a tumor suppressor by restricting the replicative potential in primary cells. Cancers evade that limitation primarily through the reactivation of telomerase via different mechanisms. Mutations within the promoter of the telomerase reverse transcriptase (TERT) gene represent a definite mechanism for the ribonucleic enzyme regeneration predominantly in cancers that arise from tissues with low rates of self-renewal. Read More

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Toxic effects of acetone, 2-pentanone, and 2-hexanone on physiological indices of wheat (Triticum aestivum L.) germination and seedlings.

Environ Sci Pollut Res Int 2021 Jul 26. Epub 2021 Jul 26.

School of Environmental Science and Engineering, China-America CRC for Environment & Health, Shandong University, 72# Jimo Binhai Road, Qingdao, Shandong, 266237, People's Republic of China.

Petroleum hydrocarbons are important characteristic pollutants in the process of oil exploitation in the Yellow River Delta (China), and they cause a potential hazard to the surrounding ecological environment. The research on eco-toxicological effects of petroleum-derived products still needs to be studied in depth. This paper describes the physiological indices of wheat (Triticum aestivum L. Read More

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Novel Small Molecule Growth Inhibitors of spp. Causing Bacterial Spot of Tomato.

Phytopathology 2021 Jul 26:PHYTO08200341R. Epub 2021 Jul 26.

Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH.

Bacterial spot (BS) of tomato, caused by , . , . , and . Read More

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The Jackson Laboratory Nathan Shock Center: impact of genetic diversity on aging.

Geroscience 2021 Jul 23. Epub 2021 Jul 23.

The Jackson Laboratory, Bar Harbor, ME, USA.

Healthspan is a complex trait, influenced by many genes and environmental factors that accelerate or delay aging, reduce or increase disease risk, and extend or reduce lifespan. Thus, assessing the role of genetic variation in aging requires an experimental strategy capable of modeling the genetic and biological complexity of human populations. The goal of the The Jackson Laboratory Nathan Shock Center (JAX NSC) is to provide research resources and training for geroscience investigators that seek to understand the role of genetics and genetic diversity on the fundamental process of aging and diseases of human aging using the laboratory mouse as a model system. Read More

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Histone Mark Profiling in Pediatric Astrocytomas Reveals Prognostic Significance of H3K9 Trimethylation and Histone Methyltransferase SUV39H1.

Neurotherapeutics 2021 Jul 22. Epub 2021 Jul 22.

Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, 75 Mikras Asias Street, 11527, Athens, Greece.

Alterations in global histone methylation regulate gene expression and participate in cancer onset and progression. The profile of histone methylation marks in pediatric astrocytomas is currently understudied with limited data on their distribution among grades. The global expression patterns of repressive histone marks H3K9me3, H3K27me3, and H4K20me3 and active H3K4me3 and H3K36me3 along with their writers SUV39H1, SETDB1, EZH2, MLL2, and SETD2 were investigated in 46 pediatric astrocytomas and normal brain tissues. Read More

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The road to success of coagulase-negative staphylococci: clinical significance of small colony variants and their pathogenic role in persistent infections.

Eur J Clin Microbiol Infect Dis 2021 Jul 23. Epub 2021 Jul 23.

Chair and Department of Medical Microbiology, Medical University of Lublin, ul. Chodźki 1, 20-093, Lublin, Poland.

Bacterial small colony variants represent an important aspect of bacterial variability. They are naturally occurring microbial subpopulations with distinctive phenotypic and pathogenic traits, reported for many clinically important bacteria. In clinical terms, SCVs tend to be associated with persistence in host cells and tissues and are less susceptible to antibiotics than their wild-type (WT) counterparts. Read More

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Pathogenesis of the inflammatory bowel disease in context of SARS-COV-2 infection.

Mol Biol Rep 2021 Jul 22. Epub 2021 Jul 22.

Chaim Sheba Medical Center, The Zabludowicz Research Center for Autoimmune Diseases, Tel Hashomer, Israel.

To date, the latest research results suggest that the novel severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) can enter host cells directly via the gastrointestinal tract by binding to the enterocyte-expressed ACE2 receptor, or indirectly as a result of infection of type II alveolar epithelial cells. At the same time, entry of SARS-CoV-2 through the gastrointestinal tract initiates the activation of innate and adaptive immune responses, the formation of an excessive inflammatory reaction and critical increase in the expression of proinflammatory cytokines, which, subsequently, can presumably increase inflammation and induce intestinal damage in patients suffering from inflammatory bowel disease (IBD). The aims of the present review were to reveal and analyze possible molecular pathways and consequences of the induction of an innate and adaptive immune response during infection with SARS-CoV-2 in patients with IBD. Read More

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Human placental piwi-interacting RNA transcriptome is characterized by expression from the DLK1-DIO3 imprinted region.

Sci Rep 2021 Jul 22;11(1):14981. Epub 2021 Jul 22.

British Columbia Cancer Research Centre, 675 West 10th Ave, Vancouver, BC, Canada.

The placenta is vital to embryonic development and requires a finely-tuned pattern of gene expression, achieved in part by its unique epigenetic landscape. Piwi-interacting RNAs (piRNAs) are a class of small-non-coding RNA with established roles as epigenetic regulators of gene expression, largely via methylation of targeted DNA sequences. The expression of piRNAs have mainly been described in germ cells, but a fraction have been shown to retain expression in adult somatic tissues. Read More

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Impact of COVID-19 Pandemic on Infectious Keratitis Outcomes: A Retrospective Multicenter Study in Tertiary Eye Hospitals of South India.

Cornea 2021 Jul 22. Epub 2021 Jul 22.

Cornea and Refractive Services, Aravind Eye Hospital, Pondicherry, India; Cornea and External Diseases Division, Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD; Cornea and Refractive Services, Aravind Eye Hospital, Coimbatore, India; Cornea and Refractive Services, Aravind Eye Hospital, Tirunelveli, India; Cornea and Refractive Services, Aravind Eye Hospital, Chennai, India; Cornea and Refractive Services, Aravind Eye Hospital, Salem, India; and Cornea and Refractive Services, Aravind Eye Hospital, Madurai, India.

Purpose: The purpose of this study was to report the adverse effects of the COVID-19 pandemic on the clinical outcomes of infectious keratitis in South India.

Methods: Two hundred fifty-eight patients presented with infectious keratitis at 6 tertiary care centers when strict travel restrictions were in place from March 24 to May 31, 2020. Case records were collected retrospectively and analyzed for demographics, type of initial treatment, length of delay in presentation, microbiological diagnosis, clinical course, and final treatment outcome. Read More

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A comprehensive evaluation and impact of normalization of generalized tracer kinetic model parameters to characterize blood-brain-barrier permeability in normal-appearing and tumor tissue regions of patients with glioma.

Magn Reson Imaging 2021 Jul 23. Epub 2021 Jul 23.

Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New Delhi, India; Department of Biomedical Engineering, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

Rationale And Objectives: To comprehensively evaluate robustness and variations of DCE-MRI derived generalized-tracer-kinetic-model(GTKM) parameters in healthy and tumor tissues and impact of normalization in mitigating these variations on application to glioma.

Materials (patients) And Methods: A retrospective study included pre-operative 31 high-grade-glioma(HGG), 22 low-grade-glioma(LGG) and 33 follow-up data from 10 patients a prospective study with 4 HGG subjects. Voxel-wise GTKM was fitted to DCE-MRI data to estimate K, v, v. Read More

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p28-functionalized PLGA nanoparticles loaded with gefitinib reduce tumor burden and metastases formation on lung cancer.

J Control Release 2021 Jul 23. Epub 2021 Jul 23.

i3S- Instituto de Inovação e Investigação em Saúde, University of Porto, R. Alfredo Allen, 208, 4200-135 Porto, Portugal; INEB-Instituto de Engenharia Biomédica, University of Porto, R. Alfredo Allen, 208, 4200-135 Porto, Portugal; CESPU-Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, R. Central de Gandra, 1317, 4585-116, Gandra, Portugal. Electronic address:

Lung cancer is still the main cause of cancer-related deaths worldwide. Its treatment generally includes surgical resection, immunotherapy, radiotherapy, and chemo-targeted therapies such as the application of tyrosine kinase inhibitors. Gefitinib (GEF) is one of them, but its poor solubility in gastric fluids weakens its bioavailability and therapeutic activity. Read More

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