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Glycans unique to the relapse-prone subset within triple-negative breast cancer as revealed by lectin array-based analysis of surgical specimens.

PLoS One 2021 11;16(5):e0250747. Epub 2021 May 11.

Division of Glycobiologics, Intractable Disease Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan.

Introduction: Molecular and cellular characteristics of the relapse-prone subset within triple-negative breast cancer (TNBC) remain unclear. Aberrant glycosylation is involved in the malignant behavior of cancer cells. In the present study, we aimed to reveal glycan profiles unique to relapsed TNBC patients. Read More

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Intrapatient Tumor Heterogeneity in IHC Interpretation Using PD-L1 IHC 22C3 pharmDx.

Appl Immunohistochem Mol Morphol 2021 May 12. Epub 2021 May 12.

Agilent Technologies Inc., R&D Agilent Technologies Inc., Histopathology, Carpinteria, CA.

Tumor heterogeneity may impact immunohistochemical (IHC) interpretation, thus potentially affecting decision making by treating oncologists for cancer patient management. Programmed cell death ligand-1 (PD-L1) IHC 22C3 pharmDx is a companion diagnostic used as an aid in identifying patient eligibility for treatment with pembrolizumab (KEYTRUDA). This study aims to investigate tumor heterogeneity impact on IHC staining when evaluating PD-L1 expression using PD-L1 IHC 22C3 pharmDx. Read More

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Genetic predisposition in the 2'-5'A pathway in the development of type 1 diabetes: potential contribution to dysregulation of innate antiviral immunity.

Diabetologia 2021 May 11. Epub 2021 May 11.

The Bartholin Institute, Department of Pathology, Rigshospitalet, Copenhagen, Denmark.

Aims/hypothesis: The incidence of type 1 diabetes is increasing more rapidly than can be explained by genetic drift. Viruses may play an important role in the disease, as they seem to activate the 2'-5'-linked oligoadenylate (2'-5'A) pathway of the innate antiviral immune system. Our aim was to investigate this possibility. Read More

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Oxidant therapy improves adipogenic differentiation of adipose-derived stem cells in human wound healing.

Stem Cell Res Ther 2021 May 10;12(1):280. Epub 2021 May 10.

Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria.

Background: Adipose-derived stem cells (ASC) and adipocytes are involved in numerous physiological and pathophysiological conditions, which have been extensively described in subcutaneous and visceral fat depots over the past two decades. However, much less is known about ASC and adipocytes outside classical fat tissue depots and their necessity in tissue remodeling after injury. Therefore, we investigated the etiology of adipocytes in human granulation tissue and define their possible role wound healing. Read More

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Grape seed extract effects on hippocampal neurogenesis, synaptogenesis and dark neurons production in old mice. Can this extract improve learning and memory in aged animals?

Nutr Neurosci 2021 May 10:1-11. Epub 2021 May 10.

Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: During the elderly, hippocampal neurogenesis and synaptogenesis reduce and dark neurons (DNs) increase, leading to cognitive impairment. It is believed that natural products can protect the neural cells and system by protecting from damages or promoting regeneration. Therefore, the effects of grape seed extract (GSE) on the hippocampus of aged mice were investigated in this study. Read More

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Necrosis binding of Ac-Lys(IRDye800CW)-Tyr-octreotate: a consequence from cyanine-labeling of small molecules.

EJNMMI Res 2021 May 10;11(1):47. Epub 2021 May 10.

Department of Radiology and Nuclear Medicine/Molecular Genetics, Erasmus Medical Centre, 's-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands.

Background: There is a growing body of nuclear contrast agents that are repurposed for fluorescence-guided surgery. New contrast agents are obtained by substituting the radioactive tag with, or adding a fluorescent cyanine to the molecular structure of antibodies or peptides. This enables intra-operative fluorescent detection of cancerous tissue, leading to more complete tumor resection. Read More

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First Report of Alfalfa Anthracnose Caused by in Xinjiang, China.

Plant Dis 2021 May 10. Epub 2021 May 10.

Bazhou Agricultural Technology Promotion Center, plant protection , korla, xinjiang, China;

Alfalfa ( L.) is widely planted in the world as one of the most important leguminous forage crops, and it is also the first choice of forage crops for animal feed in Xinjiang. In June 2018, alfalfa plants with typical anthracnose symptoms were observed in 75% of alfalfa fields in Hutubi County, Xinjiang, China. Read More

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First Report of Pectobacterium brasiliense causing soft rot on mizuna (Brassica rapa var. japonica) in the United States.

Plant Dis 2021 May 10. Epub 2021 May 10.

University of Hawai'i at Manoa, 3949, Plant and Environmental Protection Sciences, Honolulu, Hawaii, United States;

Mizuna (Brassica rapa var. japonica), a member of family Brassicaceae, is a leafy vegetable having phenolic and other compounds beneficial to human health, such as natural antioxidants (Khanam et al. 2012). Read More

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Targeting Peritumoral Lesions Identified by Computed Tomography and Magnetic Resonance Imaging in Feline Injection-Site Sarcomas for Microscopic Examination.

Vet Pathol 2021 May 10:3009858211012949. Epub 2021 May 10.

2694Oregon State University, Corvallis, OR, USA.

Peritumoral lesions identified during in vivo imaging of feline injection-site sarcoma (FISS) are frequently interpreted as neoplastic. We recently showed that most peritumoral imaging-identified lesions (PTIILs) in FISS are non-neoplastic. In this article, we describe a protocol to target PTIIL for microscopic examination and report on the protocol's performance. Read More

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Liposomal amphotericin B is more effective in polymorphic lesions of post kala-azar dermal leishmaniasis.

Indian J Dermatol Venereol Leprol 2021 Apr 23:1-6. Epub 2021 Apr 23.

Department of Pharmacology, Institute of Postgraduate Medical Education and Research, Kolkata, India.

Background: Post kala-azar dermal leishmaniasis (PKDL) is thought to be the reservoir of infection for visceral leishmaniasis in South Asia. The development of strategies for the diagnosis and treatment of PKDL are important for the implementation of the visceral leishmaniasis elimination program.

Aims: Liposomal amphotericin B (L-AMB) has been an overwhelming success in the treatment of visceral leishmaniasis. Read More

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TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.

Front Oncol 2021 22;11:643104. Epub 2021 Apr 22.

Department of Spinal Orthopedics, The First Clinical Affiliated Hospital of Guangxi Medical University, Nanning, China.

Introduction: Osteosarcoma is among the most common orthopedic neoplasms, and currently, there are no adequate biomarkers to predict its prognosis. Therefore, the present study was aimed to identify the prognostic biomarkers for autophagy-and immune-related osteosarcoma using bioinformatics tools for guiding the clinical diagnosis and treatment of this disease.

Materials And Methods: The gene expression and clinical information data were downloaded from the Public database. Read More

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Term Newborns with relatively low Tissue Oxygen Saturation Levels soon after Birth are predisposed to Neonatal Respiratory Disorders in Low-risk, Elective Cesarean Sections.

Int J Med Sci 2021 30;18(11):2262-2268. Epub 2021 Mar 30.

Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Japan.

Neonatal respiratory disorders, such as transient tachypnea of the newborn and respiratory distress syndrome, occur frequently after an elective cesarean delivery. Although conventional pulse oximetry is recommended for neonatal resuscitation, it often requires several minutes after birth to obtain a reliable signal. In a previous study, we used novel tissue oximetry equipment to detect fetal and neonatal early tissue oxygen saturation (StO) before and immediately after vaginal delivery. Read More

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Osteosarcoma of jaws: Challenges in diagnosis.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):589. Epub 2021 Jan 9.

Department of Oral Surgery, Government Dental College, Raipur, Chhattisgarh, India.

Osteosarcoma (OS) accounts for about 20% of all sarcomas with gnathic involvement seen in about 6%-10% of all OSs. The clinical presentation of OSs in the jaws is different from that of long bones as swelling is the most common complaint in patients with jaw OS followed by pain. The histopathologic variables seen are more favorable in OSs of jaws. Read More

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January 2021

Tumor budding - A promising prognostic histopathological parameter in oral squamous cell carcinoma - A comparative immunohistochemical study.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):587. Epub 2021 Jan 9.

Department of Oral Pathology and Microbiology, Vasantdada Patil Dental College and Hospital, Sangli, Maharashtra, India.

Introduction: The majority of the head and neck squamous cell carcinomas (HNSCC) occur in the oral cavity. Even with advances in cancer therapy only minor improvements in the survival of HNSCC patients have taken place and approximately 350,000 patients die annually of HNSCC worldwide. Tumor budding (TB) is a novel and promising histo-morphological parameter that has been studied in many cancers. Read More

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January 2021

Comparison of staining adequacy between tissues stored in formalin and paraffin embedded blocks for prolonged duration.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):586. Epub 2021 Jan 9.

Department of Prosthodontics, Sri Siddhartha Dental College and Hospital, Sri Siddhartha Academy of Higher Education, Tumakuru, Karnataka, India.

Introduction: Tissues submitted for histopathological diagnoses are routinely fixed in formaldehyde fixative which facilitates tissue storage for the extended period of time for further investigation or research purpose. The literature has reliable data relating to immunohistochemistry and molecular studies done using tissue fixed in formalin for longer duration and in paraffin-embedded tissue blocks. We have attempted to evaluate staining adequacy of archival specimens using hematoxylin and eosin (H&E) and trichrome stains. Read More

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January 2021

Morphometric analysis of basal cells of oral epithelium in predicting malignant transformation of oral potentially malignant disorders in patients with tobacco chewing habit.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):579. Epub 2021 Jan 9.

Department of Oral and Maxillofacial Pathology, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India.

Background: Oral potentially malignant disorders (OPMDs) are a heterogenous group of disorders which precede the development of oral cancer. These are characterized by increased risk of malignant transformation to Oral cancer.

Aims & Objectives: In this study, an attempt has been made to assess the morphological alterations of the nuclei of the basal cells in OPMDs and oral squamous cell carcinoma (OSCC). Read More

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January 2021

Immunohistochemical detection of 8-hydroxydeoxyguanosine: A biomarker of oxidative DNA damage in oral submucous fibrosis.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):536-541. Epub 2021 Jan 9.

Department of Oral and Maxillofacial Surgery, Karpaga Vinayaga Institute of Dental Sciences, Chengalpattu, Tamil Nadu, India.

Background: Oral submucous fibrosis (OSMF) is one of the common potentially malignant disorders prevailing in India. The primary etiological factors include tobacco and arecanut, which contain numerous reactive oxygen species (ROS). ROS attack guanine bases in DNA and form 8-hydroxydeoxyguanosine (8-OHdG), which can be detected in patients who have diseases associated with oxidative stress. Read More

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January 2021

Expression of CK 19 as a biomarker in early detection of oral squamous cell carcinoma.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):523-529. Epub 2021 Jan 9.

Department of Biostatistics, Amrita School of Medicine, AIMS, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India.

Background: Increased mortality in oral squamous cell carcinoma (OSCC) had been attributed to delay in diagnosis. Lack of a specific marker to assess the malignant potential of premalignant lesions is thought to be one of the reasons for late detection. Expression of Cytokeratin 19, which is widely used as an odontogenic epithelial marker had been reported in OSCC. Read More

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January 2021

"Analysis of immunohistochemical expression of epidermal growth factor receptor in oral squamous cell carcinoma using tissue microarray technology and whole sections:" A comparative study.

J Oral Maxillofac Pathol 2020 Sep-Dec;24(3):499-504. Epub 2021 Jan 9.

Department of Oral Pathology, Srinivas Institute of Dental Sciences, Mangalore, Karnataka, India.

Background: Oral squamous cell carcinoma (OSCC) accounts for more than 90% of all oral cancers. Epidermal growth factor receptor (EGFR) dysregulation is associated with essentially all of the key features of cancer. Tissue microarrays (TMAs) allow the simultaneous analysis of many tumours using small-diameter cores sampled from larger blocks of tissue. Read More

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January 2021

Digital Frozen Sections with Fluorescence Confocal Microscopy During Robot-assisted Radical Prostatectomy: Surgical Technique.

Eur Urol 2021 May 5. Epub 2021 May 5.

Department of Urology, Ospedale Policlinico e Nuovo Ospedale Civile S. Agostino Estense Modena, University of Modena and Reggio Emilia, Modena, Italy.

Background: Robot-assisted radical prostatectomy (RARP) involves a tradeoff between oncological control and functional outcomes. Intraoperative control of surgical margins (SMs) may help in ensuring the safety of the dissection. Fluorescence confocal microscopy (FCM) is an effective method for interpretation of prostate tissue and provides digital images with an appearance similar to hematoxylin-eosin staining. Read More

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Analysis of Silymarin-Modulating Effects against Acrylamide-Induced Cerebellar Damage in Male Rats: Biochemical and Pathological Markers.

J Chem Neuroanat 2021 May 6:101964. Epub 2021 May 6.

Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon. Electronic address:

Background: Acrylamide (ACR) is a well-proven neurotoxin and potential food carcinogen in humans and rodent models. Silymarin (SIL) is a flavonoid mixture isolated from seeds, leaves, and fruits of Silymarin marianum (milk thistle) that possesses a free-radical scavenging effect.

Objective: In this work, the primary focus was to investigate the efficacy of SIL to mitigate ACR-induced subacute neurotoxic effects and oxidative changes in rat cerebellum. Read More

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Ultrasound-guided fine needle aspiration cytology of angiosarcoma of head and neck: a review of cytomorphologic features and discussion of diagnostic pitfall of aspiration cytology of vascular lesions.

Diagn Cytopathol 2021 May 8. Epub 2021 May 8.

Fine Needle Aspiration Biopsy Clinic, Department of Pathology, Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai Hospital, Mount Sinai Beth Israel, New York, New York, USA.

Angiosarcoma is a rare malignant tumor of vascular origin (<2% of sarcomas). It represents <1% of all head-neck malignancies. A cytological diagnosis on fine needle aspiration (FNA) is extremely difficult. Read More

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Protective effect against d-gal-induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion.

J Food Sci 2021 May 7. Epub 2021 May 7.

Institute of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.

Defatted walnut kernel with pellicle (WKP) is an industrial byproduct during walnut oil extraction, which is rich in protein and polyphenols. WKP was hydrolyzed by simulated gastrointestinal digestion to obtain WKP hydrolysates (WKPHs). Results showed the protein recovery and hydrolysis degree of WKPH were 82. Read More

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Safety of intravenous thrombolysis in stroke of unknown time of onset: A systematic review and meta-analysis.

J Thromb Thrombolysis 2021 May 7. Epub 2021 May 7.

Cerebrovascular Disease Center, Department of Neurology, People's Hospital, China Medical University. Address, 33 Wenyi Road, Shenhe District, Shenyang, 110016, People's Republic of China.

The safety of intravenous tissue plasminogen activator (IV-tPA) in patients with stroke of unknown time of onset (SUTO) was unclear and mostly concerned. We sought to investigate the safety in terms of symptomatic intracranial hemorrhage (sICH) and death in SUTO patients treated with IV-tPA. We searched PubMed and EMBASE from inception to 2 December 2020 for eligible studies reporting IV-tPA in SUTO patients compared to conservative medical therapy, or to stroke of known onset time (SKOT) treated with IV-tPA within standard time window. Read More

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Metabolome and transcriptome profiling reveals anthocyanin contents and anthocyanin-related genes of chimeric leaves in Ananas comosus var. bracteatus.

BMC Genomics 2021 May 7;22(1):331. Epub 2021 May 7.

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China.

Background: Ananas comosus var. bracteatus is a colorful plant used as a cut flower or landscape ornamental. The unique foliage color of this plant includes both green and red leaves and, as a trait of interest, deserves investigation. Read More

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Ferulic acid positively modulates the inflammatory response to septic liver injury through the GSK-3β/NF-κB/CREB pathway.

Life Sci 2021 May 4:119584. Epub 2021 May 4.

Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, PR China. Electronic address:

Aims: Ferulic acid (FA) is a component found in plants that has free radical scavenging and liver-protective properties. Acute liver injury (ALI) is a serious complication of sepsis and is closely associated with changes in the levels of inflammatory factors. This study was taken to examine the role of FA in cecal ligation and perforation (CLP)-induced murine ALI and lipopolysaccharide (LPS)-induced cellular ALI models. Read More

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Low-Grade Oncocytic Tumor of Kidney (CK7-Positive, CD117-Negative): Incidence in a Single Institutional Experience with Clinicopathological and Molecular Characteristics.

Hum Pathol 2021 May 4. Epub 2021 May 4.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905. Electronic address:

Aims: Low-grade oncocytic tumor of the kidney (LOT) is characterized by cytoplasmic eosinophilia and a CK7-positive/CD117-negative immunophenotype. Morphologically, they exhibit overlapping features with oncocytoma and chromophobe renal cell carcinoma (chRCC). Our aim was to obtain long-term clinical follow-up data, clinicopathological and molecular characteristics, and incidence of LOT. Read More

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Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging.

Anal Chem 2021 May 7. Epub 2021 May 7.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China.

Cancer has become one of the most common diseases with high mortality in humans. Early and accurate diagnosis of cancer is of great significance to enhance the survival rate of patients. Therefore, effective molecular ligands capable of selectively recognizing cancer are urgently needed. Read More

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Validating pore size estimates in a complex microfiber environment on a human MRI system.

Magn Reson Med 2021 May 7. Epub 2021 May 7.

Cardiff University Brain Research Imaging Centre (CUBRIC), Cardiff University, Cardiff, United Kingdom.

Purpose: Recent advances in diffusion-weighted MRI provide "restricted diffusion signal fraction" and restricting pore size estimates. Materials based on co-electrospun oriented hollow cylinders have been introduced to provide validation for such methods. This study extends this work, exploring accuracy and repeatability using an extended acquisition on a 300 mT/m gradient human MRI scanner, in substrates closely mimicking tissue, that is, non-circular cross-sections, intra-voxel fiber crossing, intra-voxel distributions of pore-sizes, and smaller pore-sizes overall. Read More

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The Angiopoietin-like protein 4: a promising biomarker to distinguish brucella spondylitis from tuberculous spondylitis.

Clin Rheumatol 2021 May 6. Epub 2021 May 6.

Medical Imaging Center, Xinjiang Medical University Affiliated First Hospital, Urumqi, 830054, People's Republic of China.

Objective: The Angiopoietin-like protein 4 (ANGPTL-4) has been proved to be a protein associated with multiple inflammatory responses. Nevertheless, whether it contributes to distinguishing brucella spondylitis (BS) from tuberculous spondylitis (TS) remains an open question. Our study aim is to explore the capability of the ANGPTL-4 to differentiating BS from TS. Read More

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