15,405 results match your criteria tissue resident


Dorsal Onlay Buccal Mucosal Graft Urethroplasty In Post Radiation Female Urethral Stricture- A Technical Appraisal with Outcomes.

Urology 2021 Jun 19. Epub 2021 Jun 19.

Senior resident, Department of Urology, JIPMER, Pondicherry, India-605006. Electronic address:

Introduction And Objectives: Female urethral stricture following radiation has been reported sparsely in the literature with just a handful of case reports. Radiation to the pelvis affects the genitourinary tracts and afflicts damage by causing periurethral fibrosis, necrosis, and subsequent tissue contraction, posing as a hurdle to the reconstructive surgeon. We studied the technique and outcomes of Dorsal Onlay Buccal mucosal graft (BMG) urethroplasty in patients of radiation-induced female urethral stricture disease (FUSD). Read More

View Article and Full-Text PDF

Distinct populations of antigen specific tissue resident CD8 T cells in human cervix mucosa.

JCI Insight 2021 Jun 22. Epub 2021 Jun 22.

Department of Laboratory Medicine, and Pathology, University of Washington, Seattle, United States of America.

The ectocervix is part of the lower female reproductive tract (FRT), which is susceptible to sexually transmitted infections (STI). Comprehensive knowledge of the phenotypes and T cell receptor (TCR) repertoire of tissue resident memory T cells (TRM) in human FRT is lacking. We have taken single-cell RNA sequencing approaches to simultaneously define gene expression and TCR clonotypes of the human ectocervix. Read More

View Article and Full-Text PDF

Role of tissue-resident memory T cells in the pathophysiology of allergic contact dermatitis.

Curr Opin Allergy Clin Immunol 2021 Jun 21. Epub 2021 Jun 21.

CIRI, Centre International de Recherche en Infectiologie team Epidermal Immunity and Allergy , INSERM, U1111, Univ Lyon, Université de Lyon 1, Ecole Normale Supérieure de Lyon, CNRS, UMR 5308, Lyon Université de Lyon, Centre Hospitalier Lyon-Sud, Service d'Allergologie et d'Immunologie Clinique, Pierre-Benite, France.

Purpose Of Review: We bring updated knowledge on tissue-resident memory T cells (TRM), underlining their major role in the recurrence and the severity of allergic contact dermatitis (ACD).

Recent Findings: ACD is a frequently encountered skin disease. It is defined as a delayed-type hypersensitivity reaction initiated by the recruitment of antigen-specific T cells into the skin of sensitized patients. Read More

View Article and Full-Text PDF

Visceral adipose tissue imparts peripheral macrophage influx into the hypothalamus.

J Neuroinflammation 2021 Jun 21;18(1):140. Epub 2021 Jun 21.

Division of Biomedical Sciences, School of Medicine, University of California, Riverside, Riverside, CA, 92521, USA.

Background: Obesity is characterized by a systemic inflammation and hypothalamic neuroinflammation. Systemic inflammation is caused by macrophages that infiltrate obese adipose tissues. We previously demonstrated that high-fat diet (HFD)-fed male mice exhibited peripheral macrophage infiltration into the hypothalamus, in addition to activation of resident microglia. Read More

View Article and Full-Text PDF

Oligodendrocyte progenitor cell fate and function in development and disease.

Curr Opin Cell Biol 2021 Jun 18;73:35-40. Epub 2021 Jun 18.

Department of Genetics and Genome Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA. Electronic address:

Differentiation of oligodendrocyte progenitor cells (OPCs) into myelination-capable mature oligodendrocytes is essential for proper function of the central nervous system. OPCs are tissue-resident stem cells that populate all regions of the central nervous system and exist beyond development into adulthood. Disorders that lead to disruption of this critical cell state change cause devastating myelin diseases that are often associated with shortened life span. Read More

View Article and Full-Text PDF

Ca/calmodulin kinase II-dependent regulation of β-spectrin modulates cardiac fibroblast gene expression, proliferation, and contractility.

J Biol Chem 2021 Jun 18:100893. Epub 2021 Jun 18.

The Frick Center for Heart Failure and Arrhythmia, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA; Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH, USA; Department of Internal Medicine, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address:

Fibrosis is a pronounced feature of heart disease and the result of dysregulated activation of resident cardiac fibroblasts (CFs). Recent work identified stress-induced degradation of the cytoskeletal protein β-spectrin as an important step in CF activation and cardiac fibrosis. Further, loss of β-spectrin was found to depend on Ca/calmodulin-dependent kinase II (CaMKII). Read More

View Article and Full-Text PDF

A Decade of Complications Following Total Temporomandibular Joint Reconstruction (TJR) in a Patient with Ehlers-Danlos Syndrome. Another Surgical Misadventure or An Absolute Contraindication for TJR?

J Oral Maxillofac Surg 2021 May 19. Epub 2021 May 19.

Associate Professor of Surgery, Chief of Oral & Maxillofacial Surgery, University of Cincinnati, Department of Surgery, Division of Oral & Maxillofacial Surgery, Cincinnati, OH. Electronic address:

This report describes an extensive surgical journey for a patient with Ehlers-Danlos syndrome (EDS) who underwent a total temporomandibular joint reconstruction and illustrates an ongoing challenge for oral and maxillofacial surgeons treating patients with connective tissue disorders and managing chronic pain symptoms. The surgical team attempted multiple procedures including 2 failed total temporomandibular joint replacements and a myocutaneous vascularized free flap. This case demonstrates the potential for postoperative complications in patients with Ehlers-Danlos syndrome. Read More

View Article and Full-Text PDF

Thirteen Synchronous Multifocal Calcifying Epithelial Odontogenic Tumors (CEOT): Case Report and Review of the Literature.

J Oral Maxillofac Surg 2021 May 18. Epub 2021 May 18.

Assistant Professor, Department of Diagnostic Sciences, West Virginia University School of Dentistry. Electronic address:

Background: Calcifying epithelial odontogenic tumor (CEOT, Pindborg tumor) is a rare, benign, locally aggressive neoplasm of the jaws that accounts for approximately 1% of all odontogenic tumors. It was first defined by Pindborg in 1955 and has been reported approximately 350 times in the literature; 7 reported multiple (up to 4) synchronous lesions.

Materials And Methods: We report an individual with the largest number of CEOTs reported to date and provide a literature review of multifocal CEOT cases. Read More

View Article and Full-Text PDF

IFNγ-producing CD8 tissue resident memory T cells are a targetable hallmark of immune checkpoint inhibitor-colitis.

Gastroenterology 2021 Jun 17. Epub 2021 Jun 17.

Translational Gastroenterology Unit, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom; NIHR Oxford Biomedical Research Centre, Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital, Oxford, United Kingdom. Electronic address:

Background And Aims: The pathogenesis of immune checkpoint inhibitor (ICI)-colitis remains incompletely understood. We sought to identify key cellular driver(s) of ICI-colitis and their similarities to idiopathic ulcerative colitis (UC), and to determine potential novel therapeutic targets.

Methods: We used a cross-sectional approach to study patients with ICI-colitis, those receiving ICI without the development of colitis, idiopathic UC and healthy controls. Read More

View Article and Full-Text PDF

Differentiation and functional plasticity of gamma-delta (γδ) T cells under homeostatic and disease conditions.

Mol Immunol 2021 Jun 16;136:138-149. Epub 2021 Jun 16.

National Centre for Cell Science, NCCS Complex, SP Pune University Campus, Ganeshkhind, Pune, MH-411007, India. Electronic address:

Gamma-delta (γδ) T cells are a heterogeneous population of immune cells, which constitute <5% of total T cells in mice lymphoid tissue and human peripheral blood. However, they comprise a higher proportion of T cells in the epithelial and mucosal barrier, where they perform immune functions, help in tissue repair, and maintaining homeostasis. These tissues resident γδ T cells possess properties of innate and adaptive immune cells which enables them to perform a variety of functions during homeostasis and disease. Read More

View Article and Full-Text PDF

Understanding Polyomavirus CNS Disease - a Perspective from Mouse Models.

FEBS J 2021 Jun 19. Epub 2021 Jun 19.

Department of Microbiology and Immunology, Penn State College of Medicine, Hershey, PA, 17033, United States.

JC polyomavirus (JCPyV), a ubiquitous human pathogen, causes several devastating brain diseases in immune compromised individuals. The most notable of these JCPyV-associated CNS diseases is the frequently fatal demyelinating brain disease progressive multifocal leukoencephalopathy (PML). PML, an AIDS-defining disease in the pre-cART epoch, has emerged as a life-threatening complication in patients receiving immunomodulatory agents for autoimmune and inflammatory disorders and treatment for certain hematological malignancies. Read More

View Article and Full-Text PDF

Protective function and durability of mouse lymph node-resident memory CD8 T cells.

Elife 2021 Jun 18;10. Epub 2021 Jun 18.

Department of Pathology, The University of Iowa, Iowa City, United States.

Protective lung tissue-resident memory CD8T cells (Trm) form after influenza A virus (IAV) infection. We show that IAV infection of mice generates CD69CD103and other memory CD8T cell populations in lung-draining mediastinal lymph nodes (mLNs) from circulating naive or memory CD8T cells. Repeated antigen exposure, mimicking seasonal IAV infections, generates quaternary memory (4M) CD8T cells that protect mLN from viral infection better than 1M CD8T cells. Read More

View Article and Full-Text PDF

Long non-coding RNA: an underlying bridge linking neuroinflammation and central nervous system diseases.

Neurochem Int 2021 Jun 14:105101. Epub 2021 Jun 14.

Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China. Electronic address:

Central nervous system (CNS) diseases are responsible for a large proportion of morbidity and mortality worldwide. CNS diseases caused by intrinsic and extrinsic stimuli stimulate the resident immune cells including microglia and astrocyte, resulting in neuroinflammation that exacerbates the progression of diseases. Recent evidence reveals the aberrant expression patterns of long non-coding RNAs (lncRNAs) in the damaged tissues following CNS diseases. Read More

View Article and Full-Text PDF

Cutting into wound repair.

FEBS J 2021 Jun 17. Epub 2021 Jun 17.

Helmholtz Zentrum München, Institute of Regenerative Biology and Medicine, Munich, Germany.

The skin is home to an assortment of fibroblastic lineages that shape the wound repair response towards scars or regeneration. In this review, we discuss the distinct embryonic origins, anatomic locations, and functions of fibroblastic lineages, and how these distinct lineages of fibroblasts dictate the skin's wound response across injury depths, anatomic locations, and embryonic development to promote either scarring or regeneration. We highlight the supportive role of the fascia in dictating scarring outcomes, we then discuss recent findings that indicate fascia mobilization by its resident fibroblasts supersede the classical de novo deposition program of wound matrix formation. Read More

View Article and Full-Text PDF

Preservation of Gastrointestinal Mucosal Barrier Function and Microbiome in Patients With Controlled HIV Infection.

Front Immunol 2021 31;12:688886. Epub 2021 May 31.

St. Vincent's Clinical School, UNSW, Darlinghurst, NSW, Australia.

Background: Despite successful ART in people living with HIV infection (PLHIV) they experience increased morbidity and mortality compared with HIV-negative controls. A dominant paradigm is that gut-associated lymphatic tissue (GALT) destruction at the time of primary HIV infection leads to loss of gut integrity, pathological microbial translocation across the compromised gastrointestinal barrier and, consequently, systemic inflammation. We aimed to identify and measure specific changes in the gastrointestinal barrier that might allow bacterial translocation, and their persistence despite initiation of antiretroviral therapy (ART). Read More

View Article and Full-Text PDF

Matrix Stiffness Induces Pericyte-Fibroblast Transition Through YAP Activation.

Front Pharmacol 2021 31;12:698275. Epub 2021 May 31.

State Key Laboratory for Structural Chemistry of Unstable and Stable Species, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.

Vascular pericytes, important mural cells that retain progenitor cell properties and protect vascular integrity in healthy tissues, are often associated with tumor development, but their functions in cancer invasion remain elusive. One prominent outcome of tumor occurrence is that the microenvironment of the lesion often stiffens, which could change resident cell behavior. Here, we found pericytes are matrix stiffness-responsive and mechanical stimuli induce pericyte-fibroblast transition (PFT). Read More

View Article and Full-Text PDF

Tissue-resident macrophages provide a pro-tumorigenic niche to early NSCLC cells.

Nature 2021 Jun 16. Epub 2021 Jun 16.

Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Macrophages have a key role in shaping the tumour microenvironment (TME), tumour immunity and response to immunotherapy, which makes them an important target for cancer treatment. However, modulating macrophages has proved extremely difficult, as we still lack a complete understanding of the molecular and functional diversity of the tumour macrophage compartment. Macrophages arise from two distinct lineages. Read More

View Article and Full-Text PDF

Evaluation of barbed suture for celiorrhaphy and subcutaneous closure in bitches with pyometra submitted to ovariohysterectomy.

Acta Cir Bras 2021 14;36(5):e360502. Epub 2021 Jun 14.

Resident. School of Veterinary Medicine - Universidade Federal de Mato Grosso - Cuiaba (MT), Brazil.

Purpose: To evaluate the use of barbed sutures over the surgical time, the leukogram, the tissue thickness in which the sutures were employed (ultrasonography), the costs, and the possible complications in bitches with pyometra submitted to ovariohysterectomy (OH).

Methods: Convectional 2.0 polyglyconate suture was used in the control group (CG n = 10) and 2. Read More

View Article and Full-Text PDF

Severe acute respiratory syndrome coronavirus 2 invasion in the central nervous system: a host-virus deadlock.

Acta Virol 2021 ;65(2):115-126

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) devastation on the central nervous system (CNS) is ascertained by the present clinical findings and the noticeable signs and symptoms. The CNS involvement of the virus is not trivial; although the brain has highly protective systems, the virus has ways to breach them with a destructive potential. For successful entry of the virus, different possible routes with favorable mechanisms are used. Read More

View Article and Full-Text PDF

Basement Membrane Extracellular Matrix Proteins in Pulmonary Vascular and Right Ventricular Remodeling in Pulmonary Hypertension.

Am J Respir Cell Mol Biol 2021 Jun 15. Epub 2021 Jun 15.

Johns Hopkins University School of Medicine, 1500, Division of Pulmonary and Critical Care Medicine, Baltimore, Maryland, United States;

The extracellular matrix (ECM), a highly organized network of structural and non-structural proteins, plays a pivotal role in cellular and tissue homeostasis. Changes in the ECM are critical for normal tissue repair, while dysregulation contributes to aberrant tissue remodeling. Pulmonary arterial hypertension (PAH) is a severe disorder of the pulmonary vasculature characterized by pathologic remodeling of the pulmonary vasculature and right ventricle (RV), increased production and deposition of structural and non-structural proteins, and altered expression of ECM growth factors and proteases. Read More

View Article and Full-Text PDF

Immunophenotypic analysis of the distribution of hepatic macrophages, lymphocytes and hepatic stellate cells in the adult rat liver.

Anat Histol Embryol 2021 Jun 15. Epub 2021 Jun 15.

Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

The liver consists of parenchymal hepatocytes and non-parenchymal cells. Non-parenchymal cells, Kupffer cells, hepatic stellate cells and cholangiocytes have crucial roles in liver homeostasis and liver pathology. To establish baseline data, this study investigated immunohistochemically the distribution of non-parenchymal cells in perivenular areas (PV), periportal areas (PP) and Glisson's sheath (GS) of adult rat liver. Read More

View Article and Full-Text PDF

Maintenance of the human memory T cell repertoire by subset and tissue site.

Genome Med 2021 Jun 14;13(1):100. Epub 2021 Jun 14.

Department of Microbiology and Immunology, Columbia University, New York, NY, USA.

Background: Immune-mediated protection is mediated by T cells expressing pathogen-specific T cell antigen receptors (TCR) that are maintained at diverse sites of infection as tissue-resident memory T cells (TRM) or that disseminate as circulating effector-memory (TEM), central memory (TCM), or terminal effector (TEMRA) subsets in blood and tissues. The relationship between circulating and tissue resident T cell subsets in humans remains elusive, and is important for promoting site-specific protective immunity.

Methods: We analyzed the TCR repertoire of the major memory CD4 and CD8T cell subsets (TEM, TCM, TEMRA, and TRM) isolated from blood and/or lymphoid organs (spleen, lymph nodes, bone marrow) and lungs of nine organ donors, and blood of three living individuals spanning five decades of life. Read More

View Article and Full-Text PDF

Autograft long head biceps tendon can be used as a scaffold for biologically augmenting rotator cuff repairs.

Arthroscopy 2021 Jun 11. Epub 2021 Jun 11.

Laboratory of Orthopaedic Tissue Regeneration & Orthobiologics, Department of Bioengineering, Clemson University, Clemson, SC; Frank H. Stelling and C. Dayton Riddle Orthopaedic Education and Research Laboratory, Clemson University Biomedical Engineering Innovation Campus, Greenville, SC.

Purpose: To create a viable, mechanically expanded autograft long head biceps tendon (LHBT) scaffold for biologically augmenting the repair of torn rotator cuffs.

Methods: The proximal aspect of the tenotomized LHBTs were harvested from patients during rotator cuff repair surgery and were mechanically formed into porous scaffolds using a surgical graft expander. LHBT scaffolds were evaluated for change in area, tensile properties, and tenocyte viability before and after expansion. Read More

View Article and Full-Text PDF

Immune cells in lens injury repair and fibrosis.

Exp Eye Res 2021 Jun 11;209:108664. Epub 2021 Jun 11.

Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA; Department of Ophthalmology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA. Electronic address:

Immune cells, both tissue resident immune cells and those immune cells recruited in response to wounding or degenerative conditions, are essential to both the maintenance and restoration of homeostasis in most tissues. These cells are typically provided to tissues by their closely associated vasculatures. However, the lens, like many of the tissues in the eye, are considered immune privileged sites because they have no associated vasculature. Read More

View Article and Full-Text PDF

Prognostic image-based quantification of CD8CD103 T cell subsets in high-grade serous ovarian cancer patients.

Oncoimmunology 2021 Jun 6;10(1):1935104. Epub 2021 Jun 6.

Department of Obstetrics and Gynecology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

CD103-positive tissue resident memory-like CD8 T cells (CD8CD103 TRM) are associated with improved prognosis across malignancies, including high-grade serous ovarian cancer (HGSOC). However, whether quantification of CD8, CD103 or both is required to improve existing survival prediction and whether all HGSOC patients or only specific subgroups of patients benefit from infiltration, remains unclear. To address this question, we applied image-based quantification of CD8 and CD103 multiplex immunohistochemistry in the intratumoral and stromal compartments of 268 advanced-stage HGSOC patients from two independent clinical institutions. Read More

View Article and Full-Text PDF

Joint regression-classification deep learning framework for analyzing fluorescence lifetime images using NADH and FAD.

Biomed Opt Express 2021 May 14;12(5):2703-2719. Epub 2021 Apr 14.

Laboratory for Optical and Computational Instrumentation, Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53705, USA.

In this paper, we develop a deep neural network based joint classification-regression approach to identify microglia, a resident central nervous system macrophage, in the brain using fluorescence lifetime imaging microscopy (FLIM) data. Microglia are responsible for several key aspects of brain development and neurodegenerative diseases. Accurate detection of microglia is key to understanding their role and function in the CNS, and has been studied extensively in recent years. Read More

View Article and Full-Text PDF

Microglial Lipid Biology in the Hypothalamic Regulation of Metabolic Homeostasis.

Front Endocrinol (Lausanne) 2021 27;12:668396. Epub 2021 May 27.

Diabetes Center, University of California, San Francisco, San Francisco, CA, United States.

In mammals, myeloid cells help maintain the homeostasis of peripheral metabolic tissues, and their immunologic dysregulation contributes to the progression of obesity and associated metabolic disease. There is accumulating evidence that innate immune cells also serve as functional regulators within the mediobasal hypothalamus (MBH), a critical brain region controlling both energy and glucose homeostasis. Specifically, microglia, the resident parenchymal myeloid cells of the CNS, play important roles in brain physiology and pathology. Read More

View Article and Full-Text PDF

Noncanonical functions of glucocorticoids: A novel role for glucocorticoids in performing multiple beneficial functions in endometrial stem cells.

Cell Death Dis 2021 Jun 12;12(6):612. Epub 2021 Jun 12.

Department of Biomedical Science, Jungwon University, Goesan-gun, Chungcheongbuk-do, Republic of Korea.

Chronic stress has a negative impact on many fertility-related functions; thus, the recent decline in female fertility seems to be at least partially associated with increased stress. The secretion of glucocorticoids is a typical endocrine response to chronic stress and indirectly reduces uterine receptivity through the hypothalamus-pituitary-gonadal (HPG) axis. However, in addition to its well-known canonical role, the direct effects of chronic stress-induced glucocorticoids on various uterine functions and their underlying molecular mechanisms are complex and have not yet been revealed. Read More

View Article and Full-Text PDF

Alloplastic Temporomandibular Joint Replacement in Patients With Systemic Inflammatory Arthritis and Connective Tissue Disorders.

J Oral Maxillofac Surg 2021 May 8. Epub 2021 May 8.

Assistant Professor, University of Toronto Department of Oral and Maxillofacial Surgery; Co-founder, Sinai Health System Centre for Excellence in TMJ Surgery; Co-director, University of Toronto Fellow ship Program in Advanced TMJ and Orthognathic Surgery.

Purpose: We present a retrospective study to report the outcomes of total temporomandibular joint (TMJ TJR) replacement with alloplastic devices in patients suffering from systemic inflammatory arthropathies.

Methods: A total of 39 patients with a diagnosis of rheumatoid arthritis (RA), ankylosing spondylitis (AS), psoriatic arthritis (PA), juvenile idiopathic rheumatoid arthritis (JIA), or systemic lupus, underwent alloplastic total joint replacement(s) (TJR) from 1999 to 2019. Maximal interincisal opening (in mm) was recorded before surgery (T0), at 1-year post-surgery (T1) and at last follow-up (T2). Read More

View Article and Full-Text PDF

Emerging role of Tissue Resident Memory T cells in vitiligo: From pathogenesis to therapeutics.

Autoimmun Rev 2021 Jun 10;20(8):102868. Epub 2021 Jun 10.

C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Tarsadi, Surat 394350, Gujarat, India. Electronic address:

Vitiligo is an acquired depigmenting disorder which affects both skin and mucous membranes and autoimmunity has been strongly suggested to play a role in loss of melanocytes. The recurrence of skin macules at the same sites where they were observed prior to the treatment, suggests the existence of Tissue Resident Memory T cells (TRMs) that persist within the skin or peripheral tissues with a longer survivability. Emerging studies have shown that reactivation of these skin TRMs results into autoreactive TRM cells in various autoimmune diseases including vitiligo. Read More

View Article and Full-Text PDF