93,689 results match your criteria tissue macrophages

The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages.

Int Immunopharmacol 2021 Jul 29;99:107977. Epub 2021 Jul 29.

Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing Medical University, Chongqing 40010, China. Electronic address:

Background: Liver ischaemia-reperfusion injury (IRI) is a major complication in the perioperative period and often leads to liver failure and even systemic inflammation. Sufficient evidence has demonstrated that isoflurane has anti-inflammatory effects. We aimed to determine whether isoflurane pretreatment protects against liver IRI and to investigate the mechanisms involved in this protection. Read More

View Article and Full-Text PDF

M-CSF and prostratin induced Mregs promote immune tolerance in transplanted mice through Arg-1 pathway.

Int Immunopharmacol 2021 Jul 29;99:108014. Epub 2021 Jul 29.

Department of Urology, Capital Medical University Beijing Chaoyang Hospital, Beijing, China. Electronic address:

Objective: Regulatory macrophages (Mregs) are a group of heterogeneous macrophages. These cells could induce immunosuppressive effects through the expression of immune regulatory molecules and cytokines.

Methods: The differentiation of Mregs was induced by treating bone marrow cells with M-CSF and prostratin in vitro. Read More

View Article and Full-Text PDF

Reactive astrocytes contribute to epileptogenesis in patients with cavernous angioma.

Epilepsy Res 2021 Jul 26;176:106732. Epub 2021 Jul 26.

Department of Pathology, Brain Research Institute, Niigata University, Japan.

Objective: Patients with cavernous angioma (CA) often suffer from severe epilepsy, and surgical resection is often performed to attenuate these epileptic seizures. Several studies have suggested that surgical removal of the surrounding hemosiderin-pigmented tissues adjacent to CA achieves better seizure control than restricted lesionectomy. Pathological examination of the resected foci reveals not only hemosiderin pigmentation but also various degrees of inflammatory change, such as hemosiderin-laden macrophages, gliosis and fibrosis. Read More

View Article and Full-Text PDF

Cross-tissue single-cell landscape of human monocytes and macrophages in health and disease.

Immunity 2021 Jul 27. Epub 2021 Jul 27.

Gustave Roussy Cancer Campus, Villejuif, France; Institut National de la Santé Et de la Recherche Médicale (INSERM) U1015, Equipe Labellisée-Ligue Nationale contre le Cancer, Villejuif, France.

Mononuclear phagocytes (MNPs) encompass dendritic cells, monocytes, and macrophages (MoMac), which exhibit antimicrobial, homeostatic, and immunoregulatory functions. We integrated 178,651 MNPs from 13 tissues across 41 datasets to generate a MNP single-cell RNA compendium (MNP-VERSE), a publicly available tool to map MNPs and define conserved gene signatures of MNP populations. Next, we generated a MoMac-focused compendium that revealed an array of specialized cell subsets widely distributed across multiple tissues. Read More

View Article and Full-Text PDF

Multiplexed Imaging for Immune Profiling on Human FFPE Material.

Methods Mol Biol 2021 ;2350:125-144

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

In this chapter, we describe the pipeline for multiplex immunohistochemical staining, multispectral image acquisition, and analysis. The protocol is dedicated for use on human formalin fixed paraffin embedded (FFPE) tissues and utilizes immune markers of dendritic cells, myeloid cells, and macrophages, as well as cytokeratin. This provides quantitative data of the (co-)expression levels and spatial localization of immune cell subtypes. Read More

View Article and Full-Text PDF
January 2021

Epigenetic Analysis of the Chromatin Landscape Identifies a Repertoire of Murine Eosinophil-Specific PU.1-Bound Enhancers.

J Immunol 2021 Jul 30. Epub 2021 Jul 30.

Division of Allergy and Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH;

Eosinophils develop in the bone marrow from hematopoietic progenitors into mature cells capable of a plethora of immunomodulatory roles via the choreographed process of eosinophilopoiesis. However, the gene regulatory elements and transcription factors (TFs) orchestrating this process remain largely unknown. The potency and resulting diversity fundamental to an eosinophil's complex immunomodulatory functions and tissue specialization likely result from dynamic epigenetic regulation of the eosinophil genome, a dynamic eosinophil regulome. Read More

View Article and Full-Text PDF

Macrophage regulation of angiogenesis in health and disease.

Semin Cell Dev Biol 2021 Jul 27. Epub 2021 Jul 27.

Department of Biology & Biochemistry, Faculty of Science, University of Bath, Claverton Down, Bath BA2 7AY, UK. Electronic address:

Macrophages are primarily known as phagocytic innate immune cells, but are, in fact, highly dynamic multi-taskers that interact with many different tissue types and have regulatory roles in development, homeostasis, tissue repair, and disease. In all of these scenarios angiogenesis is pivotal and macrophages appear to play a key role in guiding both blood vessel sprouting and remodelling wherever that occurs. Recent studies have explored these processes in a diverse range of models utilising the complementary strengths of rodent, fish and tissue culture studies to unravel the mechanisms underlying these interactions and regulatory functions. Read More

View Article and Full-Text PDF

Establishment of particulate matter-induced lung injury model in mouse.

Lab Anim Res 2021 Jul 30;37(1):20. Epub 2021 Jul 30.

College of Pharmacy, Gachon University, Incheon, 21936, South Korea.

Background: Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung diseases caused by PM. However, the induced severity of lung injury in animal models using PM varies from study to study due to disparities in the preparation of PM, and the route and number of PM administrations. Read More

View Article and Full-Text PDF

Clonal hematopoiesis and associated diseases: A review of recent findings.

Cancer Sci 2021 Jul 30. Epub 2021 Jul 30.

Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Recent genome-wide studies have revealed that aging or chronic inflammation can cause clonal expansion of cells in normal tissues. Clonal hematopoiesis has been the most intensively studied form of clonal expansion in the last decade. Clonal hematopoiesis of indeterminate potential (CHIP) is an age-related phenomenon observed in elderly individuals with no history of hematological malignancy. Read More

View Article and Full-Text PDF

Nuclear receptor Nur77: its role in chronic inflammatory diseases.

Essays Biochem 2021 Jul 30. Epub 2021 Jul 30.

Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences, and Amsterdam Gastroenterology, Endocrinology and Metabolism, University of Amsterdam, Amsterdam 1105 AZ, The Netherlands.

Nur77 is a nuclear receptor that has been implicated as a regulator of inflammatory disease. The expression of Nur77 increases upon stimulation of immune cells and is differentially expressed in chronically inflamed organs in human and experimental models. Furthermore, in a variety of animal models dedicated to study inflammatory diseases, changes in Nur77 expression alter disease outcome. Read More

View Article and Full-Text PDF

Anti-allergic effects of Asarum heterotropoides on an ovalbumin-induced allergic rhinitis murine model.

Biomed Pharmacother 2021 Jul 22;141:111944. Epub 2021 Jul 22.

Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero Yuseong-gu, Daejeon 34054, South Korea. Electronic address:

Allergic rhinitis (AR) is a common chronic respiratory disease. Asarum heterotropoides (AH) is predicted to be a treatment for allergic diseases, but its therapeutic effect is unclear. We aimed to determine the anti-allergic effects of AH in mice with ovalbumin (OVA)-induced AR. Read More

View Article and Full-Text PDF

Structural Brain Changes Associated with Overweight and Obesity.

J Obes 2021 16;2021:6613385. Epub 2021 Jul 16.

Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico.

Obesity is a global health problem with a broad set of comorbidities, such as malnutrition, metabolic syndrome, diabetes, systemic hypertension, heart failure, and kidney failure. This review describes recent findings of neuroimaging and two studies of cell density regarding the roles of overnutrition-induced hypothalamic inflammation in neurodegeneration. These studies provided consistent evidence of smaller cortical thickness or reduction in the gray matter volume in people with overweight and obesity; however, the investigated brain regions varied across the studies. Read More

View Article and Full-Text PDF

Macrophages, as a Promising Strategy to Targeted Treatment for Colorectal Cancer Metastasis in Tumor Immune Microenvironment.

Front Immunol 2021 13;12:685978. Epub 2021 Jul 13.

Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

The tumor immune microenvironment plays a vital role in the metastasis of colorectal cancer. As one of the most important immune cells, macrophages act as phagocytes, patrol the surroundings of tissues, and remove invading pathogens and cell debris to maintain tissue homeostasis. Significantly, macrophages have a characteristic of high plasticity and can be classified into different subtypes according to the different functions, which can undergo reciprocal phenotypic switching induced by different types of molecules and signaling pathways. Read More

View Article and Full-Text PDF

Soluble mannose receptor induces proinflammatory macrophage activation and metaflammation.

Proc Natl Acad Sci U S A 2021 Aug;118(31)

Cellular Immunology, Life and Medical Sciences Institute, University of Bonn, 53115 Bonn, Germany;

Proinflammatory activation of macrophages in metabolic tissues is critically important in the induction of obesity-induced metaflammation. Here, we demonstrate that the soluble mannose receptor (sMR) plays a direct functional role in both macrophage activation and metaflammation. We show that sMR binds CD45 on macrophages and inhibits its phosphatase activity, leading to an Src/Akt/NF-κB-mediated cellular reprogramming toward an inflammatory phenotype both in vitro and in vivo. Read More

View Article and Full-Text PDF

Ureaplasma parvum alters the immune tolerogenic state in placental tissue and could cause miscarriage.

Fertil Steril 2021 Jul 26. Epub 2021 Jul 26.

Microbiology and Immunology Laboratory, Federal University of Bahia, Anísio Teixeira Campus, Vitória da Conquista, Brazil. Electronic address:

Objective: To study the inflammatory profile and genes involved in the response to bacterial infections in women who developed spontaneous abortion in the presence of Ureaplasma parvum.

Design: Cross-sectional study.

Setting: A maternal and child referral center. Read More

View Article and Full-Text PDF

Hypoxia-inducible factors individually facilitate inflammatory myeloid metabolism and inefficient cardiac repair.

J Exp Med 2021 Sep 29;218(9). Epub 2021 Jul 29.

Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL.

Hypoxia-inducible factors (HIFs) are activated in parenchymal cells in response to low oxygen and as such have been proposed as therapeutic targets during hypoxic insult, including myocardial infarction (MI). HIFs are also activated within macrophages, which orchestrate the tissue repair response. Although isoform-specific therapeutics are in development for cardiac ischemic injury, surprisingly, the unique role of myeloid HIFs, and particularly HIF-2α, is unknown. Read More

View Article and Full-Text PDF
September 2021

Detrimental role of IL-33/ST2 pathway sustaining a chronic eosinophil-dependent Th2 inflammatory response, tissue damage and parasite burden during Toxocara canis infection in mice.

PLoS Negl Trop Dis 2021 Jul 29;15(7):e0009639. Epub 2021 Jul 29.

Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Toxocariasis is a neglected disease that affects people around the world. Humans become infected by accidental ingestion of eggs containing Toxocara canis infective larvae, which upon reaching the intestine, hatch, penetrate the mucosa and migrate to various tissues such as liver, lungs and brain. Studies have indicated that Th2 response is the main immune defense mechanism against toxocariasis, however, there are still few studies related to this response, mainly the IL-33/ST2 pathway. Read More

View Article and Full-Text PDF

Oral ferroportin inhibitor vamifeport for improving iron homeostasis and erythropoiesis in β-thalassemia: current evidence and future clinical development.

Expert Rev Hematol 2021 Jul 29:1-12. Epub 2021 Jul 29.

Professor of Internal Medicine, Department of Clinical Sciences and Community, University of Milan, Milan, Italy.

Introduction: In β-thalassemia, imbalanced globin synthesis causes reduced red blood cell survival and ineffective erythropoiesis. Suppressed hepcidin levels increase ferroportin-mediated iron transport in enterocytes, causing increased iron absorption and potentially iron overload. Low hepcidin also stimulates ferroportin-mediated iron release from macrophages, increasing transferrin saturation (TSAT), potentially forming non-transferrin-bound iron, which can be toxic. Read More

View Article and Full-Text PDF

A conserved pathway of transdifferentiation in murine Kupffer cells.

Eur J Immunol 2021 Jul 29. Epub 2021 Jul 29.

Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.

Abundant long-lived liver-resident macrophages, termed Kupffer cells, are activated during chronic liver injury. They secrete both pro-inflammatory and pro-fibrotic cytokines, which act on hepatic stellate cells causing their trans-differentiation into myofibroblasts that deposit collagen. In other tissues, wound-associated macrophages go further, and transdifferentiate into fibrocytes, secreting collagen themselves. Read More

View Article and Full-Text PDF

Acute-phase protein synthesis: a key feature of innate immune functions of the liver.

Biol Chem 2021 Jul 29. Epub 2021 Jul 29.

Clinic for Gastroenterology, Hepatology and Infectious Diseases, Hospital of the Heinrich-Heine-University, Moorenstrasse 5, D-40225Düsseldorf, Germany.

The expression of acute-phase proteins (APP's) maintains homeostasis and tissue repair, but also represents a central component of the organism's defense strategy, especially in the context of innate immunity. Accordingly, an inflammatory response is accompanied by significant changes in the serum protein composition, an aspect that is also used diagnostically. As the main site of APP synthesis the liver is constantly exposed to antigens or pathogens via blood flow, but also to systemic inflammatory signals originating either from the splanchnic area or from the circulation. Read More

View Article and Full-Text PDF

Fcγ receptor activation mediates vascular inflammation and abdominal aortic aneurysm development.

Clin Transl Med 2021 Jul;11(7):e463

Renal, Vascular and Diabetes Research Lab, IIS-Fundacion Jimenez Diaz (IIS-FJD), Madrid, Spain.

Background: Abdominal aortic aneurysm (AAA), a degenerative vascular pathology characterized by permanent dilation of the aorta, is considered a chronic inflammatory disease involving innate/adaptive immunity. However, the functional role of antibody-dependent immune response against antigens present in the damaged vessel remains unresolved. We hypothesized that engagement of immunoglobulin G (IgG) Fc receptors (FcγR) by immune complexes (IC) in the aortic wall contributes to AAA development. Read More

View Article and Full-Text PDF

S100A7 as a potential diagnostic and prognostic biomarker of esophageal squamous cell carcinoma promotes M2 macrophage infiltration and angiogenesis.

Clin Transl Med 2021 Jul;11(7):e459

Department of Thoracic Surgery, State Key Laboratory of Molecular Oncology/National Cancer Center/National Clinical Research for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Dysregulated expression of S100A7 is found in several cancers and plays an important role in tumor progression; however, its carcinogenic role in esophageal squamous carcinoma (ESCC) is still poorly understood. Here, we identified that the levels of S100A7 were remarkably upregulated in 341 tumor tissues (P < .001) and 274 serum samples (P < . Read More

View Article and Full-Text PDF

Tissue response, macrophage phenotype, and intrinsic calcification induced by cardiovascular biomaterials: Can clinical regenerative potential be predicted in a rat subcutaneous implant model?

J Biomed Mater Res A 2021 Jul 29. Epub 2021 Jul 29.

Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

The host immune response to an implanted biomaterial, particularly the phenotype of infiltrating macrophages, is a key determinant of biocompatibility and downstream remodeling outcome. The present study used a subcutaneous rat model to compare the tissue response, including macrophage phenotype, remodeling potential, and calcification propensity of a biologic scaffold composed of glutaraldehyde-fixed bovine pericardium (GF-BP), the standard of care for heart valve replacement, with those of an electrospun polycarbonate-based supramolecular polymer scaffold (ePC-UPy), urinary bladder extracellular matrix (UBM-ECM), and a polypropylene mesh (PP). The ePC-UPy and UBM-ECM materials induced infiltration of mononuclear cells throughout the thickness of the scaffold within 2 days and neovascularization at 14 days. Read More

View Article and Full-Text PDF

Adamts18 Deficiency Causes Spontaneous SMG Fibrogenesis in Adult Mice.

J Dent Res 2021 Jul 29:220345211029270. Epub 2021 Jul 29.

Key Laboratory of Brain Functional Genomics (Ministry of Education and Shanghai), School of Life Sciences, East China Normal University, Shanghai, China.

Chronic sclerosing sialadenitis of the submandibular gland (also known as Küttner tumor) is characterized by concomitant swelling of the submandibular glands secondary to strong lymphocytic infiltration and fibrosis. The pathogenesis of this disease has been unclear, but it is associated with immune disorders. ADAMTS18 is a member of the ADAMTS superfamily of extracellular proteinases. Read More

View Article and Full-Text PDF

Phase 1 study of combination pexidartinib and sirolimus to target tumor associated macrophages in unresectable sarcoma and malignant peripheral nerve sheath tumors.

Clin Cancer Res 2021 Jul 28. Epub 2021 Jul 28.

Columbia University Medical Center.

Purpose: To evaluate the safety and tolerability in phase 1 first-in-human combination therapy with pexidartinib, an inhibitor of colony-stimulating factor-1 receptor, and sirolimus, an mTOR inhibitor, to target tumor associated macrophage (TAM) polarization in soft tissue sarcomas (STSs).

Experimental Design: This multicenter phase 1 study used the time-to-event continual reassessment method (TITE-CRM) to study the combination of sirolimus, doses ranging from 2-6mg, with pexidartinib, doses ranging from 600-1000mg, both provided continuously on a 28 day cycle, in patients with advanced sarcoma. A total of 24 patients (eight MPNST, three tenosynovial giant cell tumor (TGCT), five leiomyosarcoma and eight with other sarcoma subtypes) were enrolled. Read More

View Article and Full-Text PDF

Identification of uterine leiomyosarcoma-associated hub genes and immune cell infiltration pattern using weighted co-expression network analysis and CIBERSORT algorithm.

World J Surg Oncol 2021 Jul 28;19(1):223. Epub 2021 Jul 28.

Department of Gynecology, The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University and Jiangsu Shengze Hospital, 1399 Shunxin Middle Road, Suzhou, 215228, Jiangsu Province, People's Republic of China.

Background: While large-scale genomic analyses symbolize a precious attempt to decipher the molecular foundation of uterine leiomyosarcoma (ULMS), bioinformatics results associated with the occurrence of ULMS based totally on WGCNA and CIBERSORT have not yet been reported. This study aimed to screen the hub genes and the immune cell infiltration pattern in ULMS by bioinformatics methods.

Methods: Firstly, the GSE67463 dataset, including 25 ULMS tissues and 29 normal myometrium (NL) tissues, was downloaded from the public database. Read More

View Article and Full-Text PDF

rPanglaoDB: an R package to download and merge labeled single-cell RNA-seq data from the PanglaoDB database.

Bioinformatics 2021 Jul 28. Epub 2021 Jul 28.

Department of Veterinary Integrative Biosciences.

Motivation: Characterizing cells with rare molecular phenotypes is one of the promises of high throughput single-cell RNA sequencing (scRNA-seq) techniques. However, collecting enough cells with the desired molecular phenotype in a single experiment is challenging, requiring several samples preprocessing steps to filter and collect the desired cells experimentally before sequencing. Data integration of multiple public single-cell experiments stands as a solution for this problem, allowing the collection of enough cells exhibiting the desired molecular signatures. Read More

View Article and Full-Text PDF

Resident cardiac macrophages mediate adaptive myocardial remodeling.

Immunity 2021 Jul 21. Epub 2021 Jul 21.

Departmental of Medicine, Washington University School of Medicine; Department of Pathology and Immunology, Washington University School of Medicine; Department of Developmental Biology, Washington University School of Medicine. Electronic address:

Cardiac macrophages represent a heterogeneous cell population with distinct origins, dynamics, and functions. Recent studies have revealed that C-C Chemokine Receptor 2 positive (CCR2) macrophages derived from infiltrating monocytes regulate myocardial inflammation and heart failure pathogenesis. Comparatively little is known about the functions of tissue resident (CCR2) macrophages. Read More

View Article and Full-Text PDF

The Src-family kinase Lyn in immunoreceptor signaling.

Endocrinology 2021 Jul 28. Epub 2021 Jul 28.

Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, United States.

Effective regulation of immune-cell activation is critical for ensuring that the immune response, and inflammation generated for the purpose of pathogen elimination, is limited in space and time to limit tissue damage. Autoimmune disease can occur when immunoreceptor signaling is dysregulated, leading to unrestrained inflammation and organ damage. Conversely, tumors can coopt the tissue-healing and immunosuppressive functions of hematopoietic cells to promote metastasis and evade therapy. Read More

View Article and Full-Text PDF

Emodin alleviates hypertrophic scar formation by suppressing macrophage polarization and inhibiting the Notch and TGF-β pathways in macrophages.

Braz J Med Biol Res 2021 23;54(8):e11184. Epub 2021 Jul 23.

Department of Plastic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Hypertrophic scar (HS) formation is a common complication that develops after skin injury; however, there are few effective and specific therapeutic approaches for HS. Emodin has previously been reported to inhibit mechanical stress-induced HS inflammation. Here, we investigated the molecular mechanisms underlying the inhibitory effects of emodin on HS formation. Read More

View Article and Full-Text PDF