2,009 results match your criteria tissue factor-induced

A numerical bone regeneration model incorporating angiogenesis, considering oxygen-induced secretion of vascular endothelial growth factor and vascular remodeling.

J Biomech 2021 Aug 8;127:110656. Epub 2021 Aug 8.

The State Key Laboratory of Tribology, Tsinghua University, Beijing 100084, China. Electronic address:

Angiogenesis is considered playing an important role in bone regeneration. Studies have shown that angiogenesis is affected by biological factors, oxygen tension, and blood flow. In this paper, we propose a bone regeneration model with angiogenesis based on the theories of mechanobiology regulation, vascular network modeling, oxygen-induced secretion of vascular endothelial growth factor (VEGF), and vascular remodeling. Read More

View Article and Full-Text PDF

Functional Genomic Screening During Somatic Cell Reprogramming Identifies DKK3 as a Roadblock of Organ Regeneration.

Adv Sci (Weinh) 2021 07 13;8(14):2100626. Epub 2021 May 13.

Department of Internal Medicine I University Hospital Ulm Albert-Einstein Allee 23 89081 Ulm Germany.

Somatic cell reprogramming and tissue repair share relevant factors and molecular programs. Here, Dickkopf-3 (DKK3) is identified as novel factor for organ regeneration using combined transcription-factor-induced reprogramming and RNA-interference techniques. Loss of enhances the generation of induced pluripotent stem cells but does not affect de novo derivation of embryonic stem cells, three-germ-layer differentiation or colony formation capacity of liver and pancreatic organoids. Read More

View Article and Full-Text PDF

The mir-423-5p/MMP-2 Axis Regulates the Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis.

Cancers (Basel) 2021 Jul 3;13(13). Epub 2021 Jul 3.

Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan.

A chondrosarcoma is a common tumor of the soft tissue and bone that has a high propensity to metastasize to distant organs. Nerve growth factor (NGF) is capable of promoting the progression and metastasis of several different types of tumors although the effects of NGF in a chondrosarcoma are not confirmed. Here, we found that the levels of NGF and matrix metalloproteinase-2 (MMP-2) correlated with the tumor stage in patients with a chondrosarcoma. Read More

View Article and Full-Text PDF

E-type prostanoid receptor 4 drives resolution of intestinal inflammation by blocking epithelial necroptosis.

Nat Cell Biol 2021 07 8;23(7):796-807. Epub 2021 Jul 8.

Department of Medicine 1, University of Erlangen-Nuremberg, Erlangen, Germany.

Inflammatory bowel diseases present with elevated levels of intestinal epithelial cell (IEC) death, which compromises the gut barrier, activating immune cells and triggering more IEC death. The endogenous signals that prevent IEC death and break this vicious cycle, allowing resolution of intestinal inflammation, remain largely unknown. Here we show that prostaglandin E2 signalling via the E-type prostanoid receptor 4 (EP4) on IECs represses epithelial necroptosis and induces resolution of colitis. Read More

View Article and Full-Text PDF

Toward an understanding of tissue factor pathway inhibitor-2 as a novel serodiagnostic marker for clear cell carcinoma of the ovary.

J Obstet Gynaecol Res 2021 Sep 28;47(9):2978-2989. Epub 2021 Jun 28.

Department of Obstetrics and Gynecology, Nara Medical University, Kashihara, Nara, Japan.

Aims: Tissue factor pathway inhibitor (TFPI)-2 has recently emerged as a serodiagnostic marker for patients with epithelial ovarian cancer (EOC), especially clear cell carcinoma (CCC). This review discusses the biological properties of TFPI-2 and why serum levels are elevated in CCC patients.

Methods: A comprehensive literature search was conducted in PubMed up until March, 2021. Read More

View Article and Full-Text PDF
September 2021

Effect of NOS Inhibitors and Anticoagulants on Nitric Oxide Production in a Tissue-factor Induced Rat DIC Model.

In Vivo 2021 Jul-Aug;35(4):1999-2004

Department of Hematology, Kanazawa University Hospital, Kanazawa, Japan.

Background/aim: We examined the mechanism of nitric oxide (NO) production in a tissue-factor (TF)-induced disseminated intravascular coagulation (DIC) model in rats, using inducible nitric oxide synthase (iNOS) inhibitor (L-NIL), endothelial nitric oxide synthase (eNOS) inhibitor (L-NAME), Factor Xa inhibitor (DX-9065a), and thrombin inhibitor argatroban.

Materials And Methods: Experimental DIC was induced by sustained infusion of 3.75 U/kg TF for 4 h via the tail vein. Read More

View Article and Full-Text PDF

S62798, a potent TAFIa inhibitor, accelerates endogenous fibrinolysis in a murine model of pulmonary thromboembolism.

Thromb Res 2021 08 11;204:81-87. Epub 2021 Jun 11.

Cardiovascular and Metabolic Diseases Research, Institut de Recherches Servier, Suresnes, France.

Enhancement of fibrinolysis constitutes a promising approach to treat thrombotic diseases. Venous thrombosis and thromboembolism risks are associated with increased plasma levels of TAFI (Thrombin Activatable Fibrinolysis Inhibitor) as well as its active form TAFIa. A new TAFIa inhibitor, namely S62798 has been identified. Read More

View Article and Full-Text PDF

Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway.

J Dermatol Sci 2021 Jul 2;103(1):25-32. Epub 2021 Jun 2.

Strategic Research Planning & Management Office, Drug Discovery Research Dept., Kyoto R&D Center, Maruho Co., Ltd., Kyoto, Japan.

Background: Mucopolysaccharide polysulfate (MPS) is a heparinoid and MPS-containing formulations are widely used as moisturizers for dry skin and to treat peripheral vascular insufficiency. Although MPS has therapeutic effects in skin diseases with microvascular abnormalities, the effects of MPS on microvascular function remain incompletely understood.

Objective: The aim of this study was to evaluate the functional activities of MPS on human pericytes (HPC) and human dermal microvascular endothelial cells (HDMEC) in vitro, and on microvascular permeability of the skin. Read More

View Article and Full-Text PDF

Reduced hyaluronan cross-linking induces breast cancer malignancy in a CAF-dependent manner.

Cell Death Dis 2021 06 7;12(6):586. Epub 2021 Jun 7.

Department of Molecular Biology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, 200233, Shanghai, China.

Hyaluronan (HA) cross-linking is a conformational state of HA, a covalent complex between HA and heavy chains (HCs) from inter-α-trypsin inhibitor (I-α-I) mediated by tumor necrosis factor-induced protein 6 (TSG6). Cross-linked HA has been identified as a protective factor in physiological and inflammatory conditions. However, the state of HA cross-linking in tumor microenvironment has not been fully elucidated. Read More

View Article and Full-Text PDF

Reactive Oxygen Species-Induced TRPM2-Mediated Ca Signalling in Endothelial Cells.

Antioxidants (Basel) 2021 May 3;10(5). Epub 2021 May 3.

Department of Physiology and Pathophysiology, Sino-UK Joint Laboratory of Brain Function and Injury of Henan Province, Xinxiang Medical University, Xinxiang 453003, China.

Endothelial cells form the innermost layer of blood vessels with a fundamental role as the physical barrier. While regulation of endothelial cell function by reactive oxygen species (ROS) is critical in physiological processes such as angiogenesis, endothelial function is a major target for interruption by oxidative stress resulting from generation of high levels of ROS in endothelial cells by various pathological factors and also release of ROS by neutrophils. TRPM2 is a ROS-sensitive Ca-permeable channel expressed in endothelial cells of various vascular beds. Read More

View Article and Full-Text PDF

The Evaluation of Hemostatic Abnormalities Using a CWA-Small Amount Tissue Factor Induced FIX Activation Assay in Major Orthopedic Surgery Patients.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211012094

Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan.

We analyzed the utility for a clot waveform analysis (CWA) of small tissue factor induced FIX activation (sTF/FIXa) assay in patients with major orthopedic surgery (including total hip arthroplasty [THA] and total knee arthroplasty [TKA]) receiving edoxaban for the prevention of venous thromboembolism (VTE). The sTF/FIXa assay using recombinant human TF in platelet-rich plasma (PRP) and platelet-poor plasma (PPP) was performed using a CWA in the above patients to monitor the efficacy of edoxaban administration. Of 147 patients (109 THA and 38 TKA), 21 exhibited deep vein thrombosis (DVT), and 15 had massive bleeding. Read More

View Article and Full-Text PDF

Tumor necrosis factor-induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption.

FASEB J 2021 06;35(6):e21627

Department of Pediatrics, Yale School of Medicine, Yale University, New Haven, CT, USA.

Capillary endothelial cells (ECs) maintain a semi-permeable barrier between the blood and tissue by forming inter-EC tight junctions (TJs), regulating selective transport of fluid and solutes. Overwhelming inflammation, as occurs in sepsis, disrupts these TJs, leading to leakage of fluid, proteins, and small molecules into the tissues. Mechanistically, disruption of capillary barrier function is mediated by small Rho-GTPases, such as RhoA, -B, and -C, which are activated by guanine nucleotide exchange factors (GEFs) and disrupted by GTPase-activating factors (GAPs). Read More

View Article and Full-Text PDF

Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF.

J Cell Physiol 2021 May 2. Epub 2021 May 2.

Laboratory for Experimental Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.

Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Read More

View Article and Full-Text PDF

Detailed exploration of pathophysiology involving inflammatory status and bleeding symptoms between lipopolysaccharide- and tissue factor-induced disseminated intravascular coagulation in rats.

Int J Hematol 2021 Aug 27;114(2):172-178. Epub 2021 Apr 27.

Department of Hematology, Kanazawa University Hospital, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-8641, Japan.

Lipopolysaccharide (LPS) and tissue factor (TF) have frequently been used to induce disseminated intravascular coagulation (DIC) in experimental animal models. We have previously reported that the pathophysiology of DIC differs according to the inducing agents. However, inflammatory status and bleeding symptoms have not been fully compared between rat models of the two forms of DIC. Read More

View Article and Full-Text PDF

Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.

J Cereb Blood Flow Metab 2021 Apr 25:271678X211010354. Epub 2021 Apr 25.

Department of Neurology, Peking University First Hospital, Beijing, China.

Chronic cerebral hypoperfusion, a major vascular contributor to vascular cognitive impairment and dementia, can exacerbate small vessel pathology. Connexin43, the most abundant gap junction protein in brain tissue, has been found to be critically involved in the pathological changes of vascular cognitive impairment and dementia caused by chronic cerebral hypoperfusion. However, the precise mechanisms underpinning its role are unclear. Read More

View Article and Full-Text PDF

Hepatocyte Growth Factor-Induced Tendon Stem Cell Conditioned Medium Promotes Healing of Injured Achilles Tendon.

Front Cell Dev Biol 2021 7;9:654084. Epub 2021 Apr 7.

Department of Pediatric Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Tendon repair is a medical challenge. Our present study investigated the effectiveness of acellular therapy consisting of conditioned medium (CM) of tendon stem cells (TSCs) induced with hepatocyte growth factor (HGF) in promoting the healing of injured Achilles tendon in a rat model. Proteomic analysis of soluble substances in the CM was performed using an array chip, and bioinformatic analysis was carried out to evaluate interactions among the factors. Read More

View Article and Full-Text PDF

Downregulation of OIP5-AS1 affects proNGF-induced pancreatic cancer metastasis by inhibiting p75NTR levels.

Aging (Albany NY) 2021 04 3;13(7):10688-10702. Epub 2021 Apr 3.

Department of Biliary Surgery, West China Hospital of Sichuan University, Chengdu 610041, China.

We aimed to explore the mechanism by which long non-coding RNA (lncRNA) OIP5-AS1 affects proNGF (precursor nerve growth factor)-induced pancreatic cancer metastasis by targeting the miR-186-5p/NGFR axis. Bioinformatics was used to analyse whether OIP5-AS1 targets miR-186-5p/NGFR and their expression characteristics in pancreatic cancer. OIP5-AS1 and NGFR were overexpressed in pancreatic cancer, and their levels showed a significant positive correlation. Read More

View Article and Full-Text PDF

β-Carotene Inhibits Expression of Matrix Metalloproteinase-10 and Invasion in -Infected Gastric Epithelial Cells.

Molecules 2021 Mar 12;26(6). Epub 2021 Mar 12.

Department of Food and Nutrition, BK21 FOUR, College of Human Ecology, Yonsei University, Seoul 03722, Korea.

Matrix metalloproteinases (MMPs), key molecules of cancer invasion and metastasis, degrade the extracellular matrix and cell-cell adhesion molecules. MMP-10 plays a crucial role in -induced cell-invasion. The mitogen-activated protein kinase (MAPK) signaling pathway, which activates activator protein-1 (AP-1), is known to mediate MMP expression. Read More

View Article and Full-Text PDF

A Combined Activity of Thrombin and P-Selectin Is Essential for Platelet Activation by Pancreatic Cancer Cells.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Pharmaceutical Institute, University of Bonn, An der Immenburg 4, 53121 Bonn, Germany.

Pancreatic cancer patients have an elevated risk of suffering from venous thrombosis. Among several risk factors that contribute to hypercoagulability of this malignancy, platelets possess a key role in the initiation of clot formation. Although single mechanisms of platelet activation are well-known in principle, combinations thereof and their potential synergy to mediate platelet activation is, in the case of pancreatic cancer, far from being clear. Read More

View Article and Full-Text PDF

TIPE Regulates DcR3 Expression and Function by Activating the PI3K/AKT Signaling Pathway in CRC.

Front Oncol 2020 24;10:623048. Epub 2021 Feb 24.

Cancer Research Center, School of Medicine, Xiamen University, Xiamen, China.

Tumor necrosis factor-induced protein-8 (TIPE) is highly expressed in colorectal cancer (CRC). Decoy receptor 3 (DcR3) is a soluble secreted protein that can antagonize Fas ligand (FasL)-induced apoptosis and promote tumorigenesis. It remains unclear whether TIPE can regulate DcR3 expression. Read More

View Article and Full-Text PDF
February 2021

Biomaterials in Craniomaxillofacial Reconstruction: Past, Present, and Future.

J Craniofac Surg 2021 Mar-Apr 01;32(2):535-540

DeWitt Daugtry Family Department of Surgery, Division of Plastic, Aesthetic, and Reconstructive Surgery, University of Miami/Jackson Health System.

Abstract: Deformity and tissue loss involving the craniomaxillofacial region occurs frequently as a result of trauma, oncologic resection, or a congenital malformation. In order to maximize the patient's quality of life, reconstruction of the craniomaxillofacial skeleton must seek to restore aesthetics as well as function. Advances in diagnostic technology, surgical technique, instrumentation, and innovative biomaterials used have transformed the way reconstructive surgeons approach their patients' needs. Read More

View Article and Full-Text PDF

Ibudilast Mitigates Delayed Bone Healing Caused by Lipopolysaccharide by Altering Osteoblast and Osteoclast Activity.

Int J Mol Sci 2021 Jan 25;22(3). Epub 2021 Jan 25.

Bone and Joint Research Center, Chang Gung Memorial Hospital, No. 5, Fuxing Street, Guishan Dist., Taoyuan 33305, Taiwan.

Bacterial infection in orthopedic surgery is challenging because cell wall components released after bactericidal treatment can alter osteoblast and osteoclast activity and impair fracture stability. However, the precise effects and mechanisms whereby cell wall components impair bone healing are unclear. In this study, we characterized the effects of lipopolysaccharide (LPS) on bone healing and osteoclast and osteoblast activity in vitro and in vivo and evaluated the effects of ibudilast, an antagonist of toll-like receptor 4 (TLR4), on LPS-induced changes. Read More

View Article and Full-Text PDF
January 2021

Is Regulated by MiR-206, MiR-1-3p and MiR-133b.

Front Cell Dev Biol 2020 23;8:584606. Epub 2020 Dec 23.

Cell Signaling and Apoptosis Group, Vall d'Hebron Research Institute, Barcelona, Spain.

Apoptosis plays an important role during development, control of tissue homeostasis and in pathological contexts. Apoptosis is executed mainly through the intrinsic pathway or the death receptor pathway, i.e. Read More

View Article and Full-Text PDF
December 2020

Loss of β-Cytoplasmic Actin in the Intestinal Epithelium Increases Gut Barrier Permeability and Exaggerates the Severity of Experimental Colitis.

Front Cell Dev Biol 2020 23;8:588836. Epub 2020 Oct 23.

Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, United States.

Intestinal epithelial barrier is critical for the maintenance of normal gut homeostasis and disruption of this barrier may trigger or exaggerate mucosal inflammation. The actin cytoskeleton is a key regulator of barrier structure and function, controlling the assembly and permeability of epithelial adherens and tight junctions. Epithelial cells express two actin isoforms: a β-cytoplasmic actin and γ-cytoplasmic actin. Read More

View Article and Full-Text PDF
October 2020

Hypophosphatemia and FGF23 tumor-induced osteomalacia in two cases of metastatic breast cancer.

Clin Nephrol 2021 Feb;95(2):104-111

Tumor-induced osteomalacia (TIO) is a rare paraneoplastic syndrome characterized by factor-induced dysregulation of phosphate and vitamin D metabolism resulting in alterations in bone formation, leading to bone pain and fractures. While the true incidence is likely underestimated, less than 500 cases of TIO have been reported since initial description in 1947. TIO cases have classically been associated with mesenchymal tumors of bone and soft tissue, but have also rarely been linked to malignant tumors, with scant reports implicating non-mesenchymal tumors. Read More

View Article and Full-Text PDF
February 2021

[Effect of glycoside of short-horned epimedium Herb on the expressions of transforming growth factor β and basic fibroblast growth factor induced membrane by Masquelet technology].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2020 Nov;34(11):1405-1409

Department of Osteonecrosis, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, P.R.China.

Objective: To observe the expressions of transforming growth factor β (TGF-β ) and basic fibroblast growth factor (bFGF) induced membrane by Masquelet technique in rats treated with glycoside of short-horned epimedium Herb, and to explore the effect of glycoside of short-horned epimedium Herb on Masquelet induced membrane.

Methods: Sixty 3-month-old male Wistar rats were randomly divided into 3 groups with 20 rats in each group; a tibial bone defect (6 mm in length) model was established. The blank group (group A) was not treated; the control group (group B) and the experimental group (group C) were filled with vancomycin antibiotic bone cement in the drawing stage, and the bone cement was completely solidified. Read More

View Article and Full-Text PDF
November 2020

Defining an Upstream VEGF (Vascular Endothelial Growth Factor) Priming Signature for Downstream Factor-Induced Endothelial Cell-Pericyte Tube Network Coassembly.

Arterioscler Thromb Vasc Biol 2020 12 22;40(12):2891-2909. Epub 2020 Oct 22.

From the Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa.

Objective: In this work, we have sought to define growth factor requirements and the signaling basis for different stages of human vascular morphogenesis and maturation. Approach and Results: Using a serum-free model of endothelial cell (EC) tube morphogenesis in 3-dimensional collagen matrices that depends on a 5 growth factor combination, SCF (stem cell factor), IL (interleukin)-3, SDF (stromal-derived factor)-1α, FGF (fibroblast growth factor)-2, and insulin (factors), we demonstrate that VEGF (vascular endothelial growth factor) pretreatment of ECs for 8 hours (ie, VEGF priming) leads to marked increases in the EC response to the factors which includes; EC tip cells, EC tubulogenesis, pericyte recruitment and proliferation, and basement membrane deposition. VEGF priming requires VEGFR2, and the effect of VEGFR2 is selective to the priming response and does not affect factor-dependent tubulogenesis in the absence of priming. Read More

View Article and Full-Text PDF
December 2020

Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice.

BMC Pulm Med 2020 Oct 12;20(1):263. Epub 2020 Oct 12.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, China.

Background: The complement system plays a critical role as the pathogenic factor in the models of acute lung injury due to various causes. Cobra venom factor (CVF) is a commonly used complement research tool. The CVF can cause acute inflammation in the lung by producing complement activation components. Read More

View Article and Full-Text PDF
October 2020

Heat shock protein 70 positively regulates transforming growth factor-α-induced hepatocellular carcinoma cell migration via the AKT signaling pathway.

Heliyon 2020 Sep 23;6(9):e05002. Epub 2020 Sep 23.

Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Japan.

Heat shock proteins (HSPs) are induced in response to extracellular stress and manage the quality of proteins as molecular chaperones. HSP70, a highly conserved HSP, has been reported to correlate with the proliferation and migration of human cancer cells, such as oral, prostate, lung and liver cancer. Regarding hepatocellular carcinoma (HCC), the HSP70 levels in the tumor tissues from patients are significantly higher than those in the normal liver tissues. Read More

View Article and Full-Text PDF
September 2020

Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds.

Exp Ther Med 2020 Nov 27;20(5):19. Epub 2020 Aug 27.

Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.

Articular cartilage injuries are common orthopedic conditions that severely affect the quality of life of patients. Tissue engineering can facilitate cartilage repair and the key points involve scaffolding and seed cell selection. Pre-experiments found a range of microstructures of bioceramic scaffolds suitable for chondrocyte adhesion and proliferation, and maintaining chondrocyte phenotype. Read More

View Article and Full-Text PDF
November 2020