264,081 results match your criteria tissue detected


DNA Methylome Mapping Identifies Epigenetic Abnormalities in Intestinal Lymphocyte Regulation in Human Necrotizing Enterocolitis.

Dig Dis Sci 2021 Nov 30. Epub 2021 Nov 30.

Department of General Surgery, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, 200040, China.

Background: Epigenetic changes occur in response to environmental factors during the pathogenesis of necrotizing enterocolitis (NEC) in animal models, but the DNA methylation signature in human patients with NEC has not been examined.

Aim: To illustrate the signature and function of DNA methylation in the intestine of human NEC.

Methods: DNA methyltransferases (DNMTs) were compared between intestinal tissue with NEC and control. Read More

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November 2021

sp. nov. isolated from trachea of , sp. nov. and sp. nov. from human faeces.

Int J Syst Evol Microbiol 2021 Nov;71(11)

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320/zg-336, zg-917/zg-910 and zg-913/zg-915) isolated from animal tissues and human faeces were found to belong to the genus based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320/zg-336 had the highest 16S rRNA gene similarity to DSM 44353 (97.51 %), zg-917/zg-910 to DSM 44184 (98. Read More

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November 2021

Expression of CTAG1B clone EPR13780 versus gene rearrangement distinguishes myxoid liposarcoma from its mimics with detection of novel gene copy number variations.

J Histotechnol 2021 Nov 30:1-10. Epub 2021 Nov 30.

Department of Pathology, National Cancer Institute, Cairo University, Cairo, Egypt.

Myxoid liposarcoma (MLPS) has different patterns that are often difficult to distinguish from other soft tissue lesions. MLPS is characterized by a reciprocal translocation involving the DNA Damage Inducible Transcript 3 gene that can be detected using fluorescent in situ hybridization (FISH). Recently, the marker for cancer testis antigen 1b (CTAG1B) was found to be expressed in MLPS. Read More

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November 2021

Hyperpolarization-activated cyclic nucleotide-gated channels working as pacemaker channels in colonic interstitial cells of Cajal.

J Cell Mol Med 2021 Nov 29. Epub 2021 Nov 29.

Department of Physiology, College of Medicine, Chosun University, Gwangju, Korea.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels function as pacemaker channels in spontaneously active cells. We studied the existence of HCN channels and their functional roles in the interstitial cells of Cajal (ICC) from the mouse colon using electrophysiological, immunohistochemical and molecular techniques. HCN1 and HCN3 channels were detected in anoctamin-1 (Ca -activated Cl channel; ANO1)-positive cells within the muscular and myenteric layers in colonic tissues. Read More

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November 2021

CaMKII inhibitor KN-93 impaired angiogenesis and aggravated cardiac remodelling and heart failure via inhibiting NOX2/mtROS/p-VEGFR2 and STAT3 pathways.

J Cell Mol Med 2021 Nov 29. Epub 2021 Nov 29.

Department of Cardiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Persistent cardiac Ca /calmodulin-dependent Kinase II (CaMKII) activation was considered to promote heart failure (HF) development, some studies believed that CaMKII was a target for therapy of HF. However, CaMKII was an important mediator for the ischaemia-induced coronary angiogenesis, and new evidence confirmed that angiogenesis inhibited cardiac remodelling and improved heart function, and some conditions which impaired angiogenesis aggravated ventricular remodelling. This study aimed to investigate the roles and the underlying mechanisms of CaMKII inhibitor in cardiac remodelling. Read More

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November 2021

A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae.

Methods Mol Biol 2022 ;2316:89-96

Scientific Directorate of Phytopathology, Laboratory of Virology, Benaki Phytopathological Institute, Athens, Greece.

A simplified dot-blot hybridization protocol for Potato spindle tuber viroid (PSTVd) detection in Solanaceae species is described here. The protocol uses an RNA DIG-labeled probe and a simplified extraction procedure that avoids the use of hazardous chemicals. PSTVd was detected in composite tomato leaf samples in a ratio of up to 1:15 of PSTVd-infected to non-infected tissue and in composite potato tuber samples in a ratio up to 1:5 of PSTVd-infected to non-infected tissue. Read More

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January 2022

Differential Regulation of Neurite Outgrowth and Growth Cone Morphology by 3D Fibronectin and Fibronectin-Collagen Extracellular Matrices.

Mol Neurobiol 2021 Nov 30. Epub 2021 Nov 30.

Department of Molecular Biology, Princeton University, Princeton, NJ, 08544, USA.

The extracellular matrix (ECM) plays a critical role in development, homeostasis, and regeneration of tissue structures and functions. Cell interactions with the ECM are dynamic and cells respond to ECM remodeling by changes in morphology and motility. During nerve regeneration, the ECM facilitates neurite outgrowth and guides axons with target specificity. Read More

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November 2021

CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression.

Cell Death Discov 2021 Nov 29;7(1):370. Epub 2021 Nov 29.

Department of Cardiology, Minhang Hospital, Fudan University, Shanghai, China.

Pyroptosis has been implicated in the pathophysiology of myocardial infarction (MI) in rodents, but its contribution to reperfusion injury in MI patients is unclear. Here, we evaluated pyroptosis in MI patients in vitro and in vivo models of myocardial ischemia/reperfusion (I/R) injury. We also investigated the molecular mechanisms that regulate pyroptosis and myocardial I/R injury in these in vitro and in vivo models. Read More

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November 2021

Autophagy in Spinocerebellar ataxia type 2, a dysregulated pathway, and a target for therapy.

Cell Death Dis 2021 Nov 29;12(12):1117. Epub 2021 Nov 29.

ABC-RI, Algarve Biomedical Center Research Institute, Algarve Biomedical Center, Faro, Portugal.

Spinocerebellar ataxia type 2 (SCA2) is an incurable and genetic neurodegenerative disorder. The disease is characterized by progressive degeneration of several brain regions, resulting in severe motor and non-motor clinical manifestations. The mutation causing SCA2 disease is an abnormal expansion of CAG trinucleotide repeats in the ATXN2 gene, leading to a toxic expanded polyglutamine segment in the translated ataxin-2 protein. Read More

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November 2021

tubulin alpha 1c promotes aerobic glycolysis and cell growth through upregulation of yes association protein expression in breast cancer.

Anticancer Drugs 2021 Nov 29. Epub 2021 Nov 29.

Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan Department of respiratory medicine, Jingzhou first people's Hospital Department of breast surgery, Jingzhou first people's Hospital, Jingzhou, Hubei, China.

Tubulin alpha 1c (TUBA1C) as a member of α-tubulin was identified to take part in the occurrence and development of hepatocellular carcinoma and pancreatic cancer. Using the bioinformatics, we noticed that TUBA1C level was also increased in breast cancer was also demonstrated. Here, we explored TUBA1 role in modulation of breast cancer cell aerobic glycolysis, growth and migration and explored whether yes association protein (YAP) was involved. Read More

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November 2021

CircATXN7 contributes to the progression and doxorubicin resistance of breast cancer via modulating miR-149-5p/HOXA11 pathway.

Anticancer Drugs 2021 Nov 29. Epub 2021 Nov 29.

Department of Thyroid and Breast Surgery, Xian Yang Central Hospital, Xianyang, Shanxi, China.

Breast cancer is a frequent tumor threatening the health of women. Circular RNAs (circRNAs) play vital roles in cancer progression and chemoresistance. Herein, we mainly investigate the role and potential mechanism of circRNA ataxin 7 (circATXN7; circ_0066436) in breast cancer. Read More

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November 2021

Circ_0005033 is an oncogene in laryngeal squamous cell carcinoma and regulates cell progression and Cisplatin sensitivity via miR-107/IGF1R axis.

Anticancer Drugs 2021 Nov 29. Epub 2021 Nov 29.

Department of Otolaryngology, Affiliated Hospital of Shandong Medical College, Qingdao Department of Otolaryngology, The First People's Hospital of Jiangxia District of Wuhan, Union Jiangnan Hospital, Wuhan, China.

Transcriptome expression profiles of laryngeal squamous cell carcinoma (LSCC) are altered, and we aimed to investigate expression and role of hsa_circ_0005033 (circ_0005033), microRNA (miR)-107 and insulin-like growth factor 1 receptor (IGF1R) in LSCC. Real-time PCR, western blotting and immunohistochemistry detected RNA and protein expression levels. Functional assays were performed using MTT assay, EdU assay, apoptosis assay, flow cytometry, Transwell assay, and xenograft tumor model. Read More

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November 2021

Targeting the IL-6-Yap-Snail signalling axis in synovial fibroblasts ameliorates inflammatory arthritis.

Ann Rheum Dis 2021 Nov 29. Epub 2021 Nov 29.

Arthritis and Regenerative Medicine Laboratory, Aberdeen Centre for Arthritis and Musculoskeletal Health, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK

Objective: We aimed to understand the role of the transcriptional co-factor Yes-associated protein (Yap) in the molecular pathway underpinning the pathogenic transformation of synovial fibroblasts (SF) in rheumatoid arthritis (RA) to become invasive and cause joint destruction.

Methods: Synovium from patients with RA and mice with antigen-induced arthritis (AIA) was analysed by immunostaining and qRT-PCR. SF were targeted using and mice, crossed with fluorescent reporters for cell tracing and mice for conditional ablation. Read More

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November 2021

Tracing TET1 expression in prostate cancer: discovery of malignant cells with a distinct oncogenic signature.

Clin Epigenetics 2021 Nov 29;13(1):211. Epub 2021 Nov 29.

Clinic of Urology, Pediatric Urology and Andrology, Justus-Liebig-University Giessen, Giessen, Germany.

Background: Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is involved in DNA demethylation and transcriptional regulation, plays a key role in the maintenance of stem cell pluripotency, and is dysregulated in malignant cells. The identification of cancer stem cells (CSCs) driving tumor growth and metastasis is the primary objective of biomarker discovery in aggressive prostate cancer (PCa). In this context, we analyzed TET1 expression in PCa. Read More

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November 2021

Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo.

Chin Med 2021 Nov 29;16(1):127. Epub 2021 Nov 29.

Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Honokiol (HKL) has been reported to ameliorate lipopolysaccharide (LPS)-induced acute lung injury (ALI). However, its potential mechanism of its protective effects remains unclear. In this study, the protective mechanism of HKL on LPS-induced ALI was explored in vivo and in vitro. Read More

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November 2021

Seed-Borne can Initiate Cercospora Leaf Spot from Sugar Beet ( L.) Fruit Tissue.

Phytopathology 2021 Nov 29. Epub 2021 Nov 29.

United States Department of Agriculture, Fargo, North Dakota, United States;

Cercospora leaf spot (CLS) is a globally important disease of sugar beet (Beta vulgaris L.) caused by the fungus Cercospora beticola. Long-distance movement of C. Read More

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November 2021

Assessing bone maturity: Compositional and mechanical properties of rib cortical bone at different ages.

Bone 2021 Nov 26:116265. Epub 2021 Nov 26.

Cranfield University, Defence Academy of the UK, Shrivenham, UK. Electronic address:

Understanding what maturity entails for bone, when it arrives, and its pre- and post-maturity traits and properties is very important for understanding its evolution and physiology. There is a clear but fine distinction between the chronological age of bone (the age of its donor) and the tissue age of the bone packets it comprises at the microscopic level. Whole bone fragility changes with age due to mass and architecture effects, but so do the properties of bone at the tissue level. Read More

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November 2021

Conditional Gene Targeting Using UCP1-Cre Mice Directly Targets the Central Nervous System Beyond Thermogenic Adipose Tissues.

Mol Metab 2021 Nov 26:101405. Epub 2021 Nov 26.

Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Fraternal Order of Eagles Diabetes Research Center, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Iowa Neuroscience Institute, University of Iowa Carver College of Medicine, Iowa City, IA, 52242, USA; Department of Veterans Affairs Medical Center, Iowa City, IA, 52242, USA. Electronic address:

Objective: Uncoupling protein 1 (UCP1) is a mitochondrial protein critical for adaptive thermogenesis in adipose tissues, and as such, is typically believed to be restricted to thermogenic adipose tissues. UCP1-Cre transgenic mice are utilized in numerous studies to provide "brown adipose-specific" conditional gene targeting. Here, we examine the distribution of Cre and UCP1 throughout the body in UCP1-Cre reporter mice. Read More

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November 2021

Methionine and leucine induce ARID1A degradation to promote mTOR expression and milk synthesis in mammary epithelial cells.

J Nutr Biochem 2021 Nov 26:108924. Epub 2021 Nov 26.

College of Life Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address:

Amino acids can activate mTOR to promote milk synthesis in mammary epithelial cells (MECs), but the underlying molecular mechanism is still largely unknown. The objective is to investigate the regulatory mechanism of amino acids (Met and Leu) in stimulating mRNA expression of mTOR in MECs. We found that the protein abundance of AT-rich interaction domain 1A (ARID1A) was poorly expressed in mouse mammary gland tissues of lactating period. Read More

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November 2021

Assessment of lipophilic fluorescence products in β-amyloid-induced cognitive decline: A parallel track in hippocampus, CSF, plasma and erythrocytes.

Exp Gerontol 2021 Nov 26:111645. Epub 2021 Nov 26.

Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Oxidative stress implicates in Alzheimer's disease (AD) pathophysiology, and associates with the creation of end products of free radical reactions, are known as lipophilic fluorescent products (LFPs). This study aimed to evaluate the probable parallel alterations in the spectral properties of the LFPs in the hippocampus tissues, cerebrospinal fluid (CSF), plasma, and erythrocytes during AD model induction by intra-cerebroventricular (ICV) amyloid β-protein fragment 25-35 (Aβ) injection.

Methods: Male rats received an intra-ICV injection of Aβ. Read More

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November 2021

Selective measurement of NAPE-PLD activity via a PLA-resistant fluorogenic N-acyl-phosphatidylethanolamine analog.

J Lipid Res 2021 Nov 26:100156. Epub 2021 Nov 26.

Department of Pharmacology, Vanderbilt University, Nashville, TN, USA; Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, TN, USA. Electronic address:

N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a zinc metallohydrolase enzyme that converts NAPEs to bioactive N-acyl-ethanolamides (NAEs). Altered NAPE-PLD activity may contribute to pathogenesis of obesity, diabetes, atherosclerosis, and neurological diseases. Selective measurement of NAPE-PLD activity is challenging, however, due to alternative phospholipase pathways for NAPE hydrolysis. Read More

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November 2021

Efficacy and safety of novel glycopeptides versus vancomycin for the treatment of gram-positive bacterial infections including methicillin resistant Staphylococcus aureus: A systematic review and meta-analysis.

PLoS One 2021 29;16(11):e0260539. Epub 2021 Nov 29.

Dept. of Clinical Pharmacy, Near East University, Lefkosa, Northern Cyprus.

Objective: To compare between current evidence of novel glycopeptides against vancomycin for the treatment of gram-positive bacterial infections.

Methodology: A systematic review and meta-analysis was done. Major databases were searched for eligible randomized control trials that assessed clinical success, microbiological success and safety profile of novel glycopeptides versus vancomycin for infections caused by gram-positive bacteria. Read More

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November 2021

An optimized LC-HRMS untargeted metabolomics workflow for multi-matrices investigations in the three-spined stickleback.

PLoS One 2021 29;16(11):e0260354. Epub 2021 Nov 29.

Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, Villeurbanne, France.

Environmental metabolomics has become a growing research field to understand biological and biochemical perturbations of organisms in response to various abiotic or biotic stresses. It focuses on the comprehensive and systematic analysis of a biologic system's metabolome. This allows the recognition of biochemical pathways impacted by a stressor, and the identification of some metabolites as biomarkers of potential perturbations occurring in a body. Read More

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November 2021

Probing Loop-Mediated Isothermal Amplification (LAMP) targeting two gene-fragments of rose rosette virus.

PLoS One 2021 29;16(11):e0256510. Epub 2021 Nov 29.

North Florida Research and Education Center, University of Florida, Quincy, Florida, United States America.

This study explores the development of Loop-mediated isothermal amplification (LAMP) for detection of rose rosette virus (RRV), a technique with the potential to be translated to rose nurseries. RRV is a negative-sense, single-stranded RNA virus which is a member of the genus Emaravirus (Family Fimoviridae) and the causal agent of the rose rosette disease (RRD). Although RRV symptoms are characteristics, early visual diagnosis of RRD can be misleading and confusing since it may appear like herbicide damage. Read More

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November 2021

No Evidence of SARS-CoV-2 Infection in Neotropical Primates Sampled During COVID-19 Pandemic in Minas Gerais and Rio Grande do Sul, Brazil.

Ecohealth 2021 Nov 29. Epub 2021 Nov 29.

Medical School, Health Science Post-Graduate Program, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Campus JK - Rodovia MGT 367 - Km 583, n° 5000 - Alto da Jacuba, Diamantina, MG, 39100-000, Brazil.

In 2019, a new coronavirus disease (COVID-19) was detected in China. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was capable to infect domestic and captive mammals like cats, tigers and minks. Due to genetic similarities, concern about the infection of non-human primates (NHPs) and the establishment of a sylvatic cycle has grown in the Americas. Read More

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November 2021

Clinicopathological significance of claspin overexpression and its efficacy as a novel biomarker for the diagnosis of urothelial carcinoma.

Virchows Arch 2021 Nov 29. Epub 2021 Nov 29.

Department of Molecular Pathology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

We previously reported that claspin is a key regulator in the progression of gastric cancer and renal cell carcinoma. However, the clinicopathological significance of claspin in urothelial carcinoma (UC) has not been investigated. We analyzed the expression and distribution of claspin in UC cases by immunohistochemistry. Read More

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November 2021

Homogeneous and Reproducible Mixing of Highly Viscous Biomaterial Inks and Cell Suspensions to Create Bioinks.

Gels 2021 Nov 23;7(4). Epub 2021 Nov 23.

Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine, Technische Universität Dresden, 01307 Dresden, Germany.

Highly viscous bioinks offer great advantages for the three-dimensional fabrication of cell-laden constructs by microextrusion printing. However, no standardised method of mixing a high viscosity biomaterial ink and a cell suspension has been established so far, leading to non-reproducible printing results. A novel method for the homogeneous and reproducible mixing of the two components using a mixing unit connecting two syringes is developed and investigated. Read More

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November 2021

Production of Porous Agarose-Based Structures: Freeze-Drying vs. Supercritical CO Drying.

Gels 2021 Nov 5;7(4). Epub 2021 Nov 5.

Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.

In this work, the effect of two processes, i.e., freeze-drying and supercritical CO (SC-CO) drying, on the final morphology of agarose-based porous structures, was investigated. Read More

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November 2021

Antibiotic Susceptibility, Biofilm Production, and Detection of A Gene among Isolates from Different Clinical Specimens.

Diseases 2021 Nov 1;9(4). Epub 2021 Nov 1.

Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu 44600, Nepal.

The increasing incidence of methicillin-resistant and biofilm-forming isolates in hospital settings is a gruesome concern today. The main objectives of this study were to determine the burden of in clinical samples, assess their antibiotic susceptibility pattern and detect biofilm formation and A gene in them. A total of 1968 different clinical specimens were processed to isolate following standard microbiological procedures. Read More

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November 2021

Knockdown RBM15 Inhibits Colorectal Cancer Cell Proliferation and Metastasis Via N6-Methyladenosine (m6A) Modification of MyD88 mRNA.

Cancer Biother Radiopharm 2021 Nov 25. Epub 2021 Nov 25.

Department of Gastrointestinal Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China.

Colorectal cancer (CRC) is one of the most common cancers worldwide. In this study, we explored the role of RNA binding motif protein 15 (RBM15)-mediated MyD88 mRNA N6-methyladenosine (m6A) in CRC development. Cell proliferation, apoptosis, and invasion were detected by EdU, Annexin V-FITC/PI staining, and Transwell assays, respectively. Read More

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November 2021