9,597 results match your criteria time-dependent decrease


Toxicon 2021 Jun 8. Epub 2021 Jun 8.

Programa de Pós-graduação em Farmacologia e Química Medicinal, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brazil. Electronic address:

There is no consensus on whether serotherapy prevents acute kidney injury (AKI) and there is no pharmacotherapy to impede the disease. We aimed to elaborate an AKI model induced by the administration of Bothrops jararacussu (Bj) venom for preclinical studies. Male Wistar rats were randomly divided into 3 different groups: (1) Bj-IV: intravenous administration of 0. Read More

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The Key Role of Autophagy in Silver Nanoparticle-Induced BV2 Cells Inflammation and Polarization.

Food Chem Toxicol 2021 Jun 7:112324. Epub 2021 Jun 7.

Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China. Electronic address:

As the release of silver nanoparticles (AgNPs) in the environment continues to increase, great concerns have been raised about their potential toxicity to humans. It is urgent to assess the possible toxicity of AgNPs to the immune cells of the central nervous system due to the continuous accumulation of AgNPs in the brain. This study aimed to evaluate the neurotoxicity of AgNPs and the regulatory mechanism of autophagy in AgNPs-induced inflammation by using mouse microglia BV2 cell lines. Read More

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Copper-Oxo Active Sites for Methane C-H Activation in Zeolites: Molecular Understanding of Impact of Methane Hydroxylation on UV-Vis Spectra.

Inorg Chem 2021 Jun 7. Epub 2021 Jun 7.

Department of Chemistry, University of Nevada, Reno, 1664 North Virginia Street, Reno, Nevada 89557-0216, United States.

Here, we analyze changes in the optical spectra of activated copper-exchanged zeolites during methane activation with the Tamm-Dancoff time-dependent density functional theory, TDA-DFT, while using the ωB2PLYP functional. Two active sites, [CuO] and [CuO], were studied. For [CuO], the 22 700 cm peak is associated with μ-oxo 2p → Cu 3d/4s charge transfer. Read More

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Baseline and Changes in Serum B-Cell Maturation Antigen Levels Rapidly Indicate Changes in Clinical Status Among Patients with Relapsed/Refractory Multiple Myeloma Starting New Therapy.

Target Oncol 2021 Jun 7. Epub 2021 Jun 7.

Institute for Myeloma and Bone Cancer Research, 9201 W. Sunset Blvd., Ste. 300, West Hollywood, CA, 90069, USA.

Background: B-cell maturation antigen (BCMA) is expressed on malignant plasma cells from patients with multiple myeloma (MM). These patients have higher levels of serum (s)BCMA than healthy subjects, and levels correlate with disease status. The half-life of sBCMA is only 24-36 h, and levels are independent of renal function. Read More

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Correction of Matrix Effects for Reliable Non-target Screening LC-ESI-MS Analysis of Wastewater.

Anal Chem 2021 Jun 7. Epub 2021 Jun 7.

Analytical Chemistry Group, Department of Plant and Environmental Science, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.

Matrix effects are well-known challenges for accurate and comparable measurements with liquid chromatography (LC) electrospray ionization mass spectrometry (ESI-MS). This study describes a three-step method to evaluate and compensate for matrix effects in enriched wastewater extracts using LC ESI-high-resolution MS (HRMS). As a first step, the "dilute and shoot" approach was used to determine the optimal relative enrichment factor (REF) for a direct comparison between wastewater influent (REF 10) and effluent (REF 50) extracts. Read More

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Construction of a ceRNA Network and a Prognostic lncRNA Signature associated with Vascular Invasion in Hepatocellular Carcinoma based on Weighted Gene Co-Expression Network Analysis.

J Cancer 2021 5;12(13):3754-3768. Epub 2021 May 5.

Hepatic Surgery Center, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Understanding risk factors for vascular invasion (VI) is crucial for assessing the risk of recurrence and overall prognosis of hepatocellular carcinoma (HCC). This study aimed to construct a prognostic long non-coding RNA (lncRNA) signature and a ceRNA Network associated with vascular invasion in HCC. Differentially expressed genes (DEGs) of HCC patients associated with VI were identified by analyzing data from TCGA. Read More

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Assessing time dependent changes in microbial composition of biological crime scene traces using microbial RNA markers.

Forensic Sci Int Genet 2021 May 25;53:102537. Epub 2021 May 25.

Zurich Institute of Forensic Medicine, University of Zurich, Switzerland. Electronic address:

Current body fluid identification methods do not reveal any information about the time since deposition (TsD) of biological traces, even though determining the age of traces could be crucial for the investigative process. To determine the utility of microbial RNA markers for TsD estimation, we examined RNA sequencing data from five forensically relevant body fluids (blood, menstrual blood, saliva, semen, and vaginal secretion) over seven time points, ranging from fresh to 1.5 years. Read More

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The pro-apoptotic properties of a phytonutrient rich infusion of A. cherimola leaf extract on AML cells.

Biomed Pharmacother 2021 Jun 1:111592. Epub 2021 Jun 1.

Department of Natural Sciences, Lebanese American University, Byblos, Lebanon. Electronic address:

Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds' or leaves' extracts. The present study investigates the antiproliferative and antitumor activity of Annona cherimola aqueous leaf (AAL) extract/infusion in acute myeloid leukemia (AML) cell lines in vitro. High-resolution LC-MS was first used to analyze the composition of the aqueous extract. Read More

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Dose- and time-dependent changes in viability and IL-6, CXCL8 and CCL2 production by HaCaT-cells exposed to cobalt. Effects of high and low calcium growth conditions.

PLoS One 2021 4;16(6):e0252159. Epub 2021 Jun 4.

Department of Medical Sciences, School of Medicine and Health, Örebro University, Örebro, Sweden.

Background: Sensitization requires exposure to an allergen with subsequent production of a "danger "signal. In the skin, keratinocytes are the main producers of these signals.

Objective: To compare dose- and time-effects of cobalt on the viability of and cytokine release from HaCaT cells cultured at low or high calcium. Read More

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Metal Bioaccumulation, Cytogenetic and Clinico-Biochemical Alterations in Rattus norvegicus Exposed In Situ to a Municipal Solid Waste Landfill in Lagos, Nigeria.

Biol Trace Elem Res 2021 Jun 3. Epub 2021 Jun 3.

Department of Zoology, University of Ibadan, Ibadan, Nigeria.

This study aimed at determining in animal model the health effects of in situ exposure to landfill chemicals. We evaluated metal concentrations in tissues and cytogenetic and clinico-biochemical effects in Wistar rats (Rattus norvegicus) exposed in situ at Olusosun landfill in Lagos, Nigeria. Male rats (n = 30/point) were exposed at three different points to ambient air and underground water (via drinking) at the landfill for 4-, 8-, 12-, 16-, 20- and 24-week periods. Read More

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Involvement of nitrosative stress cytotoxicity induced by CdTe quantum dots in human vascular endothelial cells.

J Toxicol Sci 2021 ;46(6):273-282

Center Laboratory, The First Affiliated Hospital, Zhejiang University School of Medicine, China.

Quantum dots (QDs) are new types of fluorescent nanomaterials which can be utilized as ideal agents for intracellular tracking, drug delivery, biomedical imaging and diagnosis. It is urgent to understand their potential toxicity and the interactions with the toxin-susceptible vascular system, especially vascular endothelial cells. In this study, we intended to explore whether the cytotoxicity of CdTe (cadmium telluride) QDs was partly induced by nitrosative stress in vascular endothelial cells. Read More

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January 2021

Affordable oral healthcare: Dental biofilm disruption using chloroplast made enzymes with chewing gum delivery.

Plant Biotechnol J 2021 Jun 2. Epub 2021 Jun 2.

Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Current approaches for oral health care rely on procedures that are unaffordable to impoverished populations, whereas aerosolized droplets in the dental clinic and poor oral hygiene may contribute to spread of several infectious diseases including COVID-19 pandemic, requiring new solutions for dental biofilm/plaque treatment at home. Plant cells have been used to produce monoclonal antibodies or antimicrobial peptides for topical applications to decrease colonization of pathogenic microbes on dental surface. Therefore, we investigated an affordable method for dental biofilm disruption by expressing lipase, dextranase or mutanase in plant cells via the chloroplast genome. Read More

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Exploratory Data Analysis of Cell and Mitochondrial High-Fat, High-Sugar Toxicity on Human HepG2 Cells.

Nutrients 2021 May 19;13(5). Epub 2021 May 19.

CNC-Center for Neuroscience and Cell Biology, CIBB-Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, UC-Biotech, Biocant Park, 3060-197 Cantanhede, Portugal.

Non-alcoholic steatohepatitis (NASH), one of the deleterious stages of non-alcoholic fatty liver disease, remains a significant cause of liver-related morbidity and mortality worldwide. In the current work, we used an exploratory data analysis to investigate time-dependent cellular and mitochondrial effects of different supra-physiological fatty acids (FA) overload strategies, in the presence or absence of fructose (F), on human hepatoma-derived HepG2 cells. We measured intracellular neutral lipid content and reactive oxygen species (ROS) levels, mitochondrial respiration and morphology, and caspases activity and cell death. Read More

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Deciphering the Molecular Mechanism Responsible for Efficiently Inhibiting Metastasis of Human Non-Small Cell Lung and Colorectal Cancer Cells Targeting the Matrix Metalloproteinases by .

Plants (Basel) 2021 May 14;10(5). Epub 2021 May 14.

Bapalal Vaidya Botanical Research Centre, Department of Biosciences, Veer Narmad South Gujarat University, Surat 394230, India.

species are known to have antimicrobial, antioxidant, anti-inflammatory, anti-diabetic as well as anticancer effects. However, no study has examined the cytotoxic and anti-metastatic efficacy of to date. Therefore, this study aimed to evaluate the potential anti-metastatic properties of ethanol crude extract of in human non-small-cell lung (A-549) and colorectal cancer (HCT-116) cells with possible mechanisms. Read More

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A Critical Review of the Time-Dependent Performance of Polymeric Pipeline Coatings: Focus on Hydration of Epoxy-Based Coatings.

Polymers (Basel) 2021 May 9;13(9). Epub 2021 May 9.

Shawcor Ltd., 25 Bethridge Road, Toronto, ON M9W 1M7, Canada.

The barrier performance of organic coatings is a direct function of mass transport and long-term stability of the polymeric structure. A predictive assessment of the protective coating cannot be conducted a priori of degradation effects on transport. Epoxy-based powder coatings are an attractive class of coatings for pipelines and other structures because application processing times are low and residual stresses between polymer layers are reduced. Read More

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Murine Macrophages Modulate Their Inflammatory Profile in Response to Gas Plasma-Inactivated Pancreatic Cancer Cells.

Cancers (Basel) 2021 May 21;13(11). Epub 2021 May 21.

ZIK plasmatis, Leibniz Institute for Plasma Science and Technology (INP Greifswald), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.

Macrophages and immuno-modulation play a dominant role in the pathology of pancreatic cancer. Gas plasma is a technology recently suggested to demonstrate anticancer efficacy. To this end, two murine cell lines were employed to analyze the inflammatory consequences of plasma-treated pancreatic cancer cells (PDA) on macrophages using the kINPen plasma jet. Read More

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Sorghum Phenolic Compounds Are Associated with Cell Growth Inhibition through Cell Cycle Arrest and Apoptosis in Human Hepatocarcinoma and Colorectal Adenocarcinoma Cells.

Foods 2021 May 1;10(5). Epub 2021 May 1.

Department of Food Nutrition Dietetics and Health, Kansas State University, Manhattan, KS 66506, USA.

Phenolic compounds in some specialty sorghums have been associated with cancer prevention. However, direct evidence and the underlying mechanisms for this are mostly unknown. In this study, phenolics were extracted from 13 selected sorghum accessions with black pericarp while F10000 hybrid with white pericarp was used as a control, and cell growth inhibition was studied in hepatocarcinoma HepG2 and colorectal adenocarcinoma Caco-2 cells. Read More

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Association between acute graft pyelonephritis and kidney graft survival: A single-center observational study.

Am J Transplant 2021 May 31. Epub 2021 May 31.

CHU Lille, Department of Nephrology, F-59000, Lille, France.

The association between acute graft pyelonephritis (AGPN) and graft failure in kidney transplant recipients (KTR) remains controversial. In this single-center observational study, we aimed to assess the incidence of AGPN as a time-dependent post-transplantation event. We also examined the association between the diagnosis of AGPN and graft outcomes. Read More

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Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins.

J Vis Exp 2021 May 17(171). Epub 2021 May 17.

Department of Pharmaceutical Sciences, University of Maryland Baltimore;

Fast Photochemical Oxidation of proteins (FPOP) coupled with mass spectrometry (MS) has become an invaluable tool in structural proteomics to interrogate protein interactions, structure, and protein conformational dynamics as a function of solvent accessibility. In recent years, the scope of FPOP, a hydroxyl radical protein foot printing (HRPF) technique, has been expanded to protein labeling in live cell cultures, providing the means to study protein interactions in the convoluted cellular environment. In-cell protein modifications can provide insight into ligand induced structural changes or conformational changes accompanying protein complex formation, all within the cellular context. Read More

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Chronic valproic acid administration enhances oxidative stress, upregulates IL6 and downregulates Nrf2, Glut1 and Glut4 in rat's liver and brain.

Neuroreport 2021 Jul;32(10):840-850

Department of Physiology, College of Medicine and Health sciences, Sultan Qaboos University, Muscat, Oman.

Valproic acid (VPA) is a powerful antiepileptic drug that was associated with several neurological and hepatic problems especially with increasing its dose and duration. These problems may be metabolic in origin and related to glucose homeostasis. So, the present study investigated the effect of different doses and durations of VPA on the expression of glucose transporters (Glut1 and Glut4), oxidative stress and inflammatory cytokine (IL-6) in the liver and specific brain regions. Read More

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Ab initio dynamics simulation of laser-induced photodissociation of phenol.

Phys Chem Chem Phys 2021 Jun 26;23(22):12718-12730. Epub 2021 May 26.

College of Physical, Sichuan University, Chengdu 610065, China.

We theoretically investigated the photodissociation dynamics of phenol molecules steered by a sequence of temporally shaped femtosecond laser pulses with high intensity and ultrashort duration, via the real-time Time-Dependent Density Functional Theory (rt-TDDFT) combined with a Molecular Dynamics (MD) simulation. The principal findings of this research are that the phenol photodissociation can take place in 50 fs; the bonds broke sequentially; the degree of phenol molecular dissociation has a strong linear correlation with the intensity. For an incident laser being 800 nm-40 fs (wavelength-pulse duration), the threshold intensity is 7 × 10 W cm and the products are hydrogen from OH1 (phenolic hydroxyl group) and CHO-fragments. Read More

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Survival models to support shared decision-making about advance care planning for people with advanced stage cystic fibrosis.

BMJ Open Respir Res 2021 May;8(1)

Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA.

Background: For people with advanced stage cystic fibrosis (CF), tailored survival estimates could facilitate preparation for decision-making in the event of acutely deteriorating respiratory function.

Methods: We used the US CF Foundation national database (2008-2013) to identify adult people with incident advanced stage CF (forced expiratory volume in 1 s (FEV1) ≤45% predicted). Using the lasso method for variable selection, we divided the dataset into training and validation samples (2:1), and developed two multivariable Cox proportional hazards models to calculate probabilities of survival from baseline (T0 model), and from 1 year after (T12 model). Read More

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Enteric glial cells exert neuroprotection from hyperglycemia-induced damage via Akt/GSK3β pathway.

Neuroreport 2021 Jul;32(10):875-881

Center for Translational Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: Enteric glial cells (EGCs) can activate multiple pathways to inhibit the deleterious effects of acute and chronic insults. Our aim was to test the effect of EGCs on hyperglycemia-induced neuron damage and its underlying intracellular mechanisms.

Methods: A coculture model composed of EGCs and neuroblastoma cells (SH-SY5Y) was established to examine glial-mediated neuroprotection under high glucose conditions. Read More

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Accelerating Cu and Cd removal in soil flushing assisted by regulating permeability with electrolytes.

Chemosphere 2021 May 17;281:130883. Epub 2021 May 17.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China. Electronic address:

Soil flushing is one of the common in-situ remediation technologies, in which the permeability of the soil determines its feasibility. Batch extractions showed that deionized water extracted about 20% Cu and 30% Cd from a soil. Electrolytes of 100 mmol/l NaCl, 500 mmol/l NaCl, and 167 mmol/l CaCl promoted the extractions to about 60% and 90%, respectively, with higher concentration and valence of cations being more effective. Read More

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Impact of Microbial Colonization of Polystyrene Microbeads on the Toxicological Responses in the Sea Urchin .

Environ Sci Technol 2021 May 21. Epub 2021 May 21.

Department of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy.

The sea urchin was exposed to either virgin or biofilm-covered polystyrene microbeads (micro-PS, 45 μm) in order to test the effect of microbial colonization on the uptake, biodistribution, and immune response. The biofilm was dominated by bacteria, as detected by scanning electron microscopy and 16S rRNA sequencing. A higher internalization rate of colonized micro-PS inside sea urchins compared to virgin ones was detected, suggesting a role of the plastisphere in the interaction. Read More

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"Don, doff, discard" to "don, doff, decontaminate"-FFR and mask integrity and inactivation of a SARS-CoV-2 surrogate and a norovirus following multiple vaporised hydrogen peroxide-, ultraviolet germicidal irradiation-, and dry heat decontaminations.

PLoS One 2021 19;16(5):e0251872. Epub 2021 May 19.

TERRA Research Centre, Gembloux Agro-Bio Tech, Liège University, Gembloux, Belgium.

Background: As the SARS-CoV-2 pandemic accelerates, the supply of personal protective equipment remains under strain. To combat shortages, re-use of surgical masks and filtering facepiece respirators has been recommended. Prior decontamination is paramount to the re-use of these typically single-use only items and, without compromising their integrity, must guarantee inactivation of SARS-CoV-2 and other contaminating pathogens. Read More

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Lipopolysaccharide induces vascular endothelial cell pyroptosis via the SP1/RCN2/ROS signaling pathway.

Eur J Cell Biol 2021 May 8;100(4):151164. Epub 2021 May 8.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, China. Electronic address:

Endothelial cell pyroptosis is a novel cause of endothelial dysfunction in sepsis. Reticulocalbin-2 (RCN2) is involved in regulating vascular inflammation and plays an important role in the cardiovascular system. However, the role of RCN2 in inflammation-induced endothelial cell pyroptosis remains to be explored. Read More

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Inhibition of Caffeine Metabolism by and Families of Plant Products in Humans: and Studies.

Front Pharmacol 2021 29;12:641090. Epub 2021 Apr 29.

Department of Biological Sciences, Faculty of Science, Simon Fraser University, Burnaby, BC, Canada.

Daily consumption of caffeinated beverages is considered safe but serious health consequences do happen in some individuals. The and families of plant (ARFP) products are popular foods and medicines in the world. We previously reported significant amounts of furanocoumarin bioactive such as 8-methoxypsoralen, 5-methoxypsoralen, and isopimpinellin in ARFP products. Read More

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Practical demonstration of time bias with administration of adjuvant therapy in lung cancer.

Lung Cancer 2021 Jul 26;157:75-78. Epub 2021 Apr 26.

Department of Radiation Oncology, Vanderbilt University Medical Center, United States.

Purpose/background: Immortal time bias (ITB) can hinder appropriate interpretations of studies administering adjuvant therapies. Given the increase in National Cancer Data Base (NCDB) analyses evaluating postoperative radiation therapy (PORT) as an adjuvant therapy, we sought to practically demonstrate the effects of ITB by performing a series of simulated NCDB analyses.

Methods: A simulated NCDB analysis was performed to examine how the reported benefit of PORT in stage III non-small cell lung cancer (NSCLC) may change with adjustment for ITB utilizing sequential land mark analysis (SLMA) and time dependent Cox (TDC) modeling. Read More

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High-fat diet impairs duodenal barrier function and elicits glia-dependent changes along the gut-brain axis that are required for anxiogenic and depressive-like behaviors.

J Neuroinflammation 2021 May 16;18(1):115. Epub 2021 May 16.

Department of Physiology and Pharmacology "V. Erspamer", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy.

Background: Mood and metabolic disorders are interrelated and may share common pathological processes. Autonomic neurons link the brain with the gastrointestinal tract and constitute a likely pathway for peripheral metabolic challenges to affect behaviors controlled by the brain. The activities of neurons along these pathways are regulated by glia, which exhibit phenotypic shifts in response to changes in their microenvironment. Read More

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