199,176 results match your criteria time pcr


The Expression and Regulation of Na+-K+-ATPase in Nasal Epithelial Cells of Chronic Rhinosinusitis with Nasal Polyps.

ORL J Otorhinolaryngol Relat Spec 2021 Sep 22:1-8. Epub 2021 Sep 22.

Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Objective: Na+-K+-ATPase (NKA) is essential in maintaining cell permeability, reserving potential energy, and preventing cellular edema. Nevertheless, how NKA expression is altered and regulated in chronic rhinosinusitis with nasal polyps (CRSwNPs) remain uncertain. Therefore, the present study aimed to explore the expression and regulation of NKA in CRSwNP. Read More

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September 2021

Increased serum exosomal long non-coding RNA SNHG15 expression predicts poor prognosis in non-small cell lung cancer.

J Clin Lab Anal 2021 Sep 22:e23979. Epub 2021 Sep 22.

Department of Respiratory and Critical Care Medicine, the First Hospital of Yulin City, Yulin City, Shaanxi Province, China.

Background: Circulating long non-coding RNAs (lncRNAs) are emerging as promising biomarkers for non-small cell lung cancer (NSCLC). This study aimed to detect serum exosomal lncRNA SNHG15 expression in NSCLC and evaluate its potential clinical value.

Methods: A total of 238 serum samples were collected from 118 patients with NSCLC, 40 patients with benign pulmonary lesions and 80 healthy volunteers. Read More

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September 2021

Evaluation of nanopore sequencing as a diagnostic tool for the rapid identification of from individual and pooled respiratory tract samples.

J Clin Microbiol 2021 Sep 22:JCM0111021. Epub 2021 Sep 22.

Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.

Rapid identification of infections in cattle is a key factor to guide antimicrobial therapy and biosecurity measures. Recently, nanopore sequencing became an affordable diagnostic tool for both clinically relevant viruses and bacteria, but the diagnostic accuracy for identification is undocumented. Therefore, in this study nanopore sequencing was compared to rapid identification of with MALDI-TOF MS (RIMM), and triplex real-time PCR in a Bayesian latent class model (BLCM) for in bronchoalveolar lavage fluid (BALf) obtained from calves. Read More

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September 2021

A Computational Framework to Infer Prostate Cancer-Associated Long Noncoding RNAs and Analyses for Identifying a Competing Endogenous RNA Network.

Genet Test Mol Biomarkers 2021 Sep;25(9):582-589

Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Prostate cancer (PC) is the second leading cause of cancer death after lung cancer in men. Current biomarkers are ineffective for the treatment and management of the disease. Long noncoding RNAs (lncRNAs) are a heterogeneous group of transcripts that are involved in complex gene expression regulatory networks. Read More

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September 2021

Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology.

Genet Test Mol Biomarkers 2021 Sep;25(9):611-614

Federal Research Center Krasnoyarsk Scientific Center, Siberian Branch of RAS, Krasnoyarsk, Russia.

To develop magnetic nanoparticles (MNPs) based on iron oxide for DNA isolation from blood cells for quantitative molecular genetic analyses of the V617F mutation in the Januskinase 2 () gene. MNPs were synthesized by the coprecipitation method and coated with tetraethyl orthosilicate (TEOS). The size and shape of the complexes were estimated using transmission electron microscopy. Read More

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September 2021

E670G (rs505151) Variant and Coronary Artery Disease Risk Among Diabetics.

Genet Test Mol Biomarkers 2021 Sep;25(9):615-623

Medical Biochemistry Department, and Faculty of Medicine, University of Zagazig, Zagazig, Egypt.

Proprotein convertase subtilisin/kexin type 9 () is an enzyme in the family of proprotein convertases implicated in lipid metabolism and is a significant genetic risk factor in cardiovascular diseases among various populations. This study explored the correlation between the alleles of the rs505151 (E670G) locus of the gene and its expression levels with coronary artery disease (CAD) risk in Egyptian patients with type 2 diabetes mellitus (T2DM). A case-control study was performed on 112 lean subjects compared to 100 T2DM patients without CAD and 84 T2DM patients with CAD to investigate the relationships among expression levels, the E670G (rs505151) gene variant, lipid concentrations, and CAD risk in an Egyptian diabetic population. Read More

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September 2021

Clinical Outcome in Patients with Nosocomial COVID-19 Infection After Thoracic Surgery.

Adv Exp Med Biol 2021 Sep 23. Epub 2021 Sep 23.

Department of Thoracic Surgery, Klinik am Park Lünen, Lünen, Germany.

In the present study, we retrospectively evaluated outcomes in 8 patients (mean age 67 ± 7, range 55-77 years; male/female 7/1) who acquired nosocomial COVID-19 infection postoperatively out of the 39 adults who underwent elective thoracic surgery in November 2020. All patients were tested negative for COVID-19 on admission. The mortality rate in the eight patients was 25%. Read More

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September 2021

In vitro virucidal activity of the theaflavin-concentrated tea extract TY-1 against influenza A virus.

J Nat Med 2021 Sep 22. Epub 2021 Sep 22.

Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido, 080-8555, Japan.

The annual spread of influenza A virus (IAV) infection is a global concern. We examined the IAV-inactivating potential of theaflavin-concentrated tea extract TY-1, which contains abundant polyphenols, including concentrated theaflavins and catechins. TY-1 exhibited concentration- and time-dependent virucidal activity against IAV. Read More

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September 2021

Silencing of Long Non-coding RNA LINC01106 Represses Malignant Behaviors of Gastric Cancer Cells by Targeting miR-34a-5p/MYCN Axis.

Mol Biotechnol 2021 Sep 22. Epub 2021 Sep 22.

Digestive System Department, The Fifth People's Hospital of Jinan, No. 24297, Jingshi Road, Jinan City, 250022, Shandong Province, China.

Long non-coding RNA LINC01106 is an lncRNA aberrantly expressed in gastric cancer (GC). However, the accurate function remains unclear. The objective of this investigation is to explore detailed regulatory mechanism of lncRNA LINC01106 in GC. Read More

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September 2021

Impeding Virulence of Candida albicans by Candesartan and Domperidone.

Curr Microbiol 2021 Sep 22. Epub 2021 Sep 22.

Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, 44519, Egypt.

Candida albicans is the most common human fungal pathogen that has developed extensive virulence factors which allows successful colonization and infection of the host. Anti-virulence agents can alleviate the pathogenesis of fungi and help the immune system to eradicate them easily. This study aimed to explore the anti-virulence effect of domperidone and candesartan against C. Read More

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September 2021

The Vancomycin Resistance-Associated Regulatory System VraSR Modulates Biofilm Formation of Staphylococcus epidermidis in an -Dependent Manner.

mSphere 2021 Sep 22:e0064121. Epub 2021 Sep 22.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan Universitygrid.8547.e, Shanghai, China.

The two-component system VraSR responds to the cell wall-active antibiotic stress in Staphylococcus epidermidis. To study its regulatory function in biofilm formation, a deletion mutant (Δ) was constructed using S. epidermidis strain 1457 (SE1457) as the parent strain. Read More

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September 2021

[Evaluation of the validity of Ag PANBIO-COVID19 in the diagnosis of SARS-CoV-2 infection in asymptomatic or mildly infected patients].

Rev Esp Quimioter 2021 Sep 22. Epub 2021 Sep 22.

Maria Paz Ventero Martín. Hospital General Universitario de Alicante, C/Pintor Baeza, 12. CP:03010. Spain.

Objective: To assess the validity of SARS-CoV-2 Antigen (Ag) detection for the diagnosis of SARS-CoV-2 infection in mildly infected or asymptomatic patients.

Methods: Observational study to evaluate diagnostic tests. Non-hospitalized patients with indication for diagnostic testing for SARS-CoV-2 infection were included. Read More

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September 2021

The roles of and methylation and elongase6 related fatty acids in metabolic syndrome.

Food Funct 2021 Sep 22. Epub 2021 Sep 22.

CAS Key Laboratory of Nutrition Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

: The prevalence of metabolic syndrome (MetS) has increased along with rapid socio-economic development in China in recent decades, aggravating the burden of the health care system. Both plasma levels of fatty acids (FAs) and aberrant DNA methylation profiles are associated with MetS risk. However, studies exploring the role of DNA methylation and FAs simultaneously in MetS etiology are sparse. Read More

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September 2021

Precise Microfluidic Luminescent Sensor Platform with Controlled Injection System.

ACS Omega 2021 Sep 2;6(36):23412-23420. Epub 2021 Sep 2.

School of Mechanical Engineering, Kyungpook National University, Daegu 41566, South Korea.

Efforts have been devoted to screening various prevalent diseases, such as severe acute respiratory syndrome (SARS) and coronavirus disease 2019 (COVID-19). Real-time polymerase chain reaction (RT-PCR), which is currently the most widely used, has high accuracy, but it requires several facilities and takes a relatively long time to check; so, new testing technology is necessary for a higher test efficiency. A chemiluminescence (CL) sensor is a relatively simple device and suitable as an alternative because it can detect very precise specimens. Read More

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September 2021

Lung Transplantation in a Patient With COVID-19-Associated Acute Respiratory Failure.

Cureus 2021 Aug 13;13(8):e17152. Epub 2021 Aug 13.

Pulmonology, University of Kentucky, Lexington, USA.

Coronavirus disease 2019 (COVID-19) is currently a significant cause of acute respiratory failure worldwide, leading to irreversible fibrotic lung disease. In patients with persistent respiratory failure after acute COVID-19 infection, lung transplant is an emerging option. Here, we have presented a case where the patient required venovenous extracorporeal membrane oxygenation (VV-ECMO) support for 33 days until a bilateral lung transplant was performed on day 71 after the initial COVID-19 infection. Read More

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Labyrinthitis: A Rare Consequence of COVID-19 Infection.

Cureus 2021 Aug 12;13(8):e17121. Epub 2021 Aug 12.

Medicine, Services Hospital, Lahore, PAK.

Since the declaration of coronavirus disease 2019 (COVID-19) as a pandemic, it remains a widespread infection with a major impact on global resources and health infrastructure. The hallmark of COVID-19 continues to be the well-documented effects it has on the respiratory system. With the passage of time, the involvement of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus in other systems has become more apparent, with the increased incidence of thromboembolic events, cardiac involvement as well as gastrointestinal and neurological symptoms secondary to the infection. Read More

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Predictors of incident SARS-CoV-2 infections in an international prospective cohort study.

BMJ Open 2021 Sep 21;11(9):e052025. Epub 2021 Sep 21.

Department of Medicine, University of California San Francisco, San Francisco, California, USA

Objective: Until effective treatments and vaccines are made readily and widely available, preventative behavioural health measures will be central to the SARS-CoV-2 public health response. While current recommendations are grounded in general infectious disease prevention practices, it is still not entirely understood which particular behaviours or exposures meaningfully affect one's own risk of incident SARS-CoV-2 infection. Our objective is to identify individual-level factors associated with one's personal risk of contracting SARS-CoV-2. Read More

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September 2021

Are circulating immune cells a determinant of pancreatic cancer risk? A prospective study using epigenetic cell count measures.

Cancer Epidemiol Biomarkers Prev 2021 Sep 20. Epub 2021 Sep 20.

Nutrition and Metabolism Branch, International Agency For Research On Cancer.

Background: Evidence is accumulating that immune cells play a prominent role in pancreatic cancer aetiology but prospective investigations are missing.

Methods: We conducted a nested case-control study within the European Prospective Investigation into Cancer and Nutrition (EPIC) study with 502 pairs of incident pancreatic cancer cases and matched controls. Relative counts of circulating immune cells (neutrophils and lymphocyte sub-lineages: total CD3+, CD8+, CD4+, and FOXP3+ regulatory T cells (Tregs) relative to nucleated cells, (white blood cells) were measured by qRT-PCR. Read More

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September 2021

Changes in PPAR-γ Expression Are Associated with microRNA Profiles during Fetal Programming due to Maternal Overweight and Obesity.

Gynecol Obstet Invest 2021 Sep 21:1-12. Epub 2021 Sep 21.

Biochemistry Department, University Autonomous of San Luis Potosí, San Luis Potosi, Mexico.

Background: There has been a global increase in the prevalence of obesity in pregnant women in recent years. Animal studies have shown that intrauterine environment associated with maternal obesity leads to epigenetic changes. However, the effects of epigenetic changes occurring before birth in response to maternal conditions have not been clearly characterized in humans. Read More

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September 2021

Comparison of the current abattoir surveillance system for detection of paratuberculosis in Australian sheep with quantitative PCR tissue strategies using simulation modelling.

Prev Vet Med 2021 Sep 15;196:105495. Epub 2021 Sep 15.

Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, NSW, Australia. Electronic address:

Abattoir surveillance for Johne's disease monitoring in Australia has provided valuable feedback to producers about their flock's disease status since its commencement in 1999. The current surveillance system relies on the identification of gross lesions in sheep carcases at an abattoir, followed by sampling and histopathology testing. This manual inspection system has not been adapted to meet the changing disease situation, as infection prevalence levels have declined over time due to vaccination. Read More

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September 2021

Pharmacokinetic/pharmacodynamic profiles of baicalin against Mycoplasma gallisepticum in an in vivo infection model.

Poult Sci 2021 Aug 20;100(11):101437. Epub 2021 Aug 20.

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, P. R. China.

Mycoplasma gallisepticum (M. gallisepticum), a devastating avian pathogen that commonly causes chronic respiratory disease in chicken, is responsible for tremendous economic losses to the poultry industry. Baicalin is the main constituent of Scutellaria baicalensis that shows potential therapeutic effects against M. Read More

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Variant-specific interventions to slow down replacement and prevent outbreaks.

Math Biosci 2021 Sep 18:108703. Epub 2021 Sep 18.

Laboratory for Industrial and Applied Mathematics, Department of Mathematics and Statistics, York University, Canada. Electronic address:

Emergency and establishment of variants of concern (VOC) impose significant challenges for the COVID-19 pandemic control specially when a large portion of the population has not been fully vaccinated. Here we develop a mathematical model and utilize this model to examine the impact of non pharmaceutical interventions, including the COVID-test (PCR, antigen and antibody test) and whole genome sequencing (WGS) test capacity and contact tracing and quarantine strength, on the VOC-induced epidemic wave. We point out the undesirable and unexpected effect of lukewarm tracing and quarantine that can potentially increase the VOC-cases at the outbreak peak time, and we demonstrate the significance of strain-specific interventions to either prevent a VOC-induced outbreak, or to mitigate the epidemic wave when this outbreak is unavoidable. Read More

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September 2021

A duplex SYBR Green I-based real time real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.

J Virol Methods 2021 Sep 18:114294. Epub 2021 Sep 18.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China. Electronic address:

Feline coronavirus (FCoV) contains two serotypes, feline enteric coronavirus (FECV) and Feline infectious peritonitis virus (FIPV). FECV and feline parvovirus (FPV) can cause similar clinical symptoms in cats, such as diarrhea. The objective of this study was to establish a duplex SYBR Green I-based quantitative polymerase chain reaction (qPCR) assay for rapid and simultaneous detection of FPV and FCoV. Read More

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September 2021

Low-dose cyclooxygenase-2 (COX-2) inhibitor celecoxib plays a protective role in the rat model of neonatal necrotizing enterocolitis.

Authors:
Ling Sun

Bioengineered 2021 Dec;12(1):7234-7245

Neonatal Intensive Care Unit, Yantaishan Hospital, Yantai, China.

This study aims to investigate the effects of the cyclooxygenase-2 (COX-2) inhibitor celecoxib on neonatal necrotizing enterocolitis (NEC) in rats. After treatment with a low dose of celecoxib (0.5, 1, or 1. Read More

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December 2021

An Advanced One-Step RT-LAMP for Rapid Detection of little cherry virus 2 Combined with HTS-based Phylogenomics Reveal Divergent Flowering Cherry Isolates.

Plant Dis 2021 Sep 21. Epub 2021 Sep 21.

ILVO, 74875, Plant Sciences, Burgemeester Van Gansberghelaan 96, Merelbeke, Merelbeke, Belgium, 9820.

Little cherry virus 2 (LChV-2, genus ) is considered to be the main causal agent of the economically damaging little cherry disease (LChD), which can only be controlled by removal of infected trees. The widespread viral disease of sweet cherry ( L.) is affecting the survival of long-standing orchards in North America and Europe, hence the dire need for an early and accurate diagnosis towards a sound disease control strategy. Read More

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September 2021

Long-term, induced expression of Hand2 in peripheral sympathetic neurons ameliorates sarcopenia in geriatric mice.

J Cachexia Sarcopenia Muscle 2021 Sep 21. Epub 2021 Sep 21.

Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.

Background: The discovery of adrenoceptors, which mediate the effects of the sympathetic nervous system neurotransmitter norepinephrine on specific tissues, sparked the development of sympathomimetics that have profound influence on skeletal muscle mass. However, chronic administration has serious side effects that preclude their use for muscle-wasting conditions such as sarcopenia, the age-dependent decline in muscle mass, force, and power. Devising interventions that can adjust neurotransmitter release to changing physiological demands will require understanding how the sympathetic nervous system affects muscle motor innervation and muscle mass, which will prevent sarcopenia-associated impaired mobility, falls, institutionalization, co-morbidity, and premature death. Read More

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September 2021

First time identification of subconjunctival Dirofilaria immitis in Turkey: giant episcleral granuloma mimicking scleritis.

Parasitol Res 2021 Sep 21. Epub 2021 Sep 21.

Department of Parasitology, Faculty of Medicine, University of Ege, Izmir, Turkey.

Dirofilariasis is a vector-borne disease that is present worldwide. This report describes a giant subconjunctival granuloma which mimics scleritis, caused by D. immitis. Read More

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September 2021

CD40 Expressed in Endothelial Cells Promotes Upregulation of ICAM-1 But Not Pro-Inflammatory Cytokines, NOS2 and P2X7 in the Diabetic Retina.

Invest Ophthalmol Vis Sci 2021 Sep;62(12):22

Division of Infectious Diseases and HIV Medicine, Dept. of Medicine, Case Western Reserve University, Cleveland, Ohio, United States.

Purpose: CD40 is an upstream inducer of inflammation in the diabetic retina. CD40 is upregulated in retinal endothelial cells in diabetes. The purpose of this study was to determine whether expression of CD40 in endothelial cells is sufficient to promote inflammatory responses in the retina of diabetic mice. Read More

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September 2021

A multiplexed ion-exchange membrane-based miRNA (MIX·miR) detection platform for rapid diagnosis of myocardial infarction.

Lab Chip 2021 Sep 21. Epub 2021 Sep 21.

Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.

Micro RNAs (miRNAs) have shown great potential as rapid and discriminating biomarkers for acute myocardial infarction (AMI) diagnosis. We have developed a multiplexed ion-exchange membrane-based miRNA (MIX·miR) preconcentration/sensing amplification-free platform for quantifying in parallel a panel of miRNAs, including miR-1, miR-208b, and miR-499, from the same plasma samples from: 1) reference subjects with no evident coronary artery disease (NCAD); 2) subjects with stable coronary artery disease (CAD); and 3) subjects experiencing ST-elevation myocardial infarction (STEMI) prior to (STEMI-pre) and following (STEMI-PCI) percutaneous coronary intervention. The picomolar limit of detection from raw plasma and 3-decade dynamic range of MIX·miR permits detection of the miRNA panel in untreated samples from disease patients and its precise standard curve, provided by large 0. Read More

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September 2021

AEOL 10150 Alleviates Radiation-induced Innate Immune Responses in Non-human Primate Lung Tissue.

Health Phys 2021 Oct;121(4):331-344

Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD 21201.

Abstract: To study the molecular and cellular mechanisms of radiation-induced lung injury (RILI) in a non-human primate model, Rhesus macaques were irradiated with lethal doses of radiation to the whole thorax. A subset of the irradiated animals was treated with AEOL 10150, a potent catalytic scavenger of reactive oxygen and nitrogen species. Lung tissues were collected at necropsy for molecular and immunohistochemical (IHC) studies. Read More

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October 2021