39 results match your criteria time neutrophils'

Pinaverium Bromide Attenuates Lipopolysaccharide-Induced Excessive Systemic Inflammation via Inhibiting Neutrophil Priming.

J Immunol 2021 Apr 26;206(8):1858-1865. Epub 2021 Mar 26.

Department of Medical Microbiology and Immunology, University of Toledo Medical Center, Toledo, OH 43614

Dominant infiltration of neutrophils is a hallmark of many inflammatory diseases, especially in septic shock. IL-1β as one of the most early released proinflammatory cytokine in neutrophil, plays a pivotal role in the progress of sepsis. In this study, we built a high-throughput-compatible drug screen assay platform based on our newly constructed reporter C57BL/6 mice, pIL1-DsRed, expressing the DsRed gene under the control of the IL-1β promoter. Read More

View Article and Full-Text PDF

Thromboinflammation Model-on-A-Chip by Whole Blood Microfluidics on Fixed Human Endothelium.

Diagnostics (Basel) 2021 Jan 29;11(2). Epub 2021 Jan 29.

Heart Research Institute, University of Sydney, Sydney, NSW 2042, Australia.

Microfluidic devices have an established role in the study of platelets and coagulation factors in thrombosis, with potential diagnostic applications. However, few microfluidic devices have assessed the contribution of neutrophils to thrombus formation, despite increasing knowledge of neutrophils' importance in cardiovascular thrombosis. We describe a thromboinflammation model which uses straight channels, lined with fixed human umbilical vein endothelial cells, after treatment with tumour necrosis factor-alpha. Read More

View Article and Full-Text PDF
January 2021

Clozapine, neutropenia and Covid-19: should clinicians be concerned? 3 months report.

Brain Behav Immun Health 2021 May 28;13:100212. Epub 2021 Jan 28.

University of Hertfordshire, De Havilland Campus, Mosquito Way, Hatfield, AL10 9EU, UK.

Background: Clozapine is among the most effective antipsychotics used for treatment resistant schizophrenia. Adverse reactions to clozapine include neutropenia. In March 2020, at the start of the Coronavirus -19 pandemic, clinicians raised concerns regarding continuation of antipsychotic treatment, and specifically of clozapine, in patients with coronavirus disease. Read More

View Article and Full-Text PDF

COVID-19 disease-Temporal analyses of complete blood count parameters over course of illness, and relationship to patient demographics and management outcomes in survivors and non-survivors: A longitudinal descriptive cohort study.

PLoS One 2020 28;15(12):e0244129. Epub 2020 Dec 28.

National Institute for Infectious Diseases, Lazzaro Spallanzani, IRCCS, Rome, Italy.

Background: Detailed temporal analyses of complete (full) blood count (CBC) parameters, their evolution and relationship to patient age, gender, co-morbidities and management outcomes in survivors and non-survivors with COVID-19 disease, could identify prognostic clinical biomarkers.

Methods: From 29 January 2020 until 28 March 2020, we performed a longitudinal cohort study of COVID-19 inpatients at the Italian National Institute for Infectious Diseases, Rome, Italy. 9 CBC parameters were studied as continuous variables [neutrophils, lymphocytes, monocytes, platelets, mean platelet volume, red blood cell count, haemoglobin concentration, mean red blood cell volume and red blood cell distribution width (RDW %)]. Read More

View Article and Full-Text PDF
January 2021

Splenic sympathetic signaling contributes to acute neutrophil infiltration of the injured spinal cord.

J Neuroinflammation 2020 Sep 23;17(1):282. Epub 2020 Sep 23.

Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.

Background: Alterations in the immune system are a complication of spinal cord injury (SCI) and have been linked to an excessive sympathetic outflow to lymphoid organs. Still unknown is whether these peripheral immune changes also contribute for the deleterious inflammatory response mounted at the injured spinal cord.

Methods: We analyzed different molecular outputs of the splenic sympathetic signaling for the first 24 h after a thoracic compression SCI. Read More

View Article and Full-Text PDF
September 2020

Human neutrophils compromise the restoration-tooth interface.

Acta Biomater 2020 11 17;117:283-293. Epub 2020 Sep 17.

Faculty of Dentistry, University of Toronto, Toronto, ON, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, ON, Canada. Electronic address:

Neutrophils, cells of the innate immune system, enter the mouth and release factors that are hypothesized to contribute to the degradation of tooth dentin, methacrylate resin composites, and adhesives at the restoration-tooth-dentin interface. The objectives were to characterize neutrophils' degradation towards resin composite, self-etch (SE) and total-etch (TE) adhesives, SE and TE resin-dentin interfaces and to identify proteins that could contribute to the degradation process. Neutrophils' degradation of cured resin composite, and SE and TE adhesives, was quantified by measuring the specific resin degradation by-product, bishydroxy-propoxy-phenyl-propane (bisHPPP), released after 30 days incubation of the materials with the cells. Read More

View Article and Full-Text PDF
November 2020

Fragile neutrophils in surgical patients: A phenomenon associated with critical illness.

PLoS One 2020 4;15(8):e0236596. Epub 2020 Aug 4.

Department of trauma surgery, University Medical Center Utrecht, Utrecht, the Netherlands.

Leukocyte viability (determined by e.g. propidium iodide [PI] staining) is automatically measured by hematology analyzers to check for delayed bench time. Read More

View Article and Full-Text PDF
October 2020

Symphytum officinale L.: Liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils.

J Ethnopharmacol 2020 Nov 31;262:113169. Epub 2020 Jul 31.

Department of Pharmacognosy, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115, Iasi, Romania; Biothermodynamics, TUM School of Life and Food Sciences Weihenstephan, Technical University of Munich, 85354, Freising, Germany. Electronic address:

Ethnopharmacological Relevance: Symphytum officinale L. (comfrey, Boraginaceae) has been traditionally used for millennia in joint distortions, myalgia, bone fractures and hematomas. However, key activity-determining constituents and molecular mechanisms underlying its use have not been completely elucidated. Read More

View Article and Full-Text PDF
November 2020

Overexpression of Gene Makes Granulocyte-Like HL-60 Susceptible to Release Reactive Oxygen Species.

Int J Mol Sci 2020 Jul 22;21(15). Epub 2020 Jul 22.

Department of Laboratory Medicine and Clinical Immunology of Developmental Age, Medical University of Warsaw, Zwirki i Wigury 63a Street, 02-091 Warsaw, Poland.

Neutrophils represent the first line of defense against pathogens using various strategies, such as phagocytosis, production of reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) formation. Recently, an autophagy-independent role of autophagy related () gene 5 in immune cells, including neutrophils, was emphasized. Our aim was to investigate the role of ATG5 protein in neutrophils' antimicrobial functions, proliferation and apoptosis. Read More

View Article and Full-Text PDF

Modelling of neutrophil dynamics in children receiving busulfan or treosulfan for haematopoietic stem cell transplant conditioning.

Br J Clin Pharmacol 2020 08 3;86(8):1537-1549. Epub 2020 Mar 3.

Department of Pharmacy, Great Ormond Street Hospital for Children, London, UK.

Aims: Busulfan and treosulfan are cytotoxic agents used in the conditioning regime prior to paediatric haematopoietic stem cell transplantation (HSCT). These agents cause suppression of myeloid cells leaving patients severely immunocompromised in the early post-HSCT period. The main objectives were: (i) to establish a mechanistic pharmacokinetic-pharmacodynamic (PKPD) model for the treatment and engraftment effects on neutrophil counts comparing busulfan and treosulfan-based conditioning, and (ii) to explore current dosing schedules with respect to time to HSCT. Read More

View Article and Full-Text PDF

Game of neutrophils: modeling the balance between apoptosis and necrosis.

BMC Bioinformatics 2019 Dec 10;20(Suppl 6):475. Epub 2019 Dec 10.

University of Amsterdam, Amsterdam, the Netherlands.

Background: Neutrophils are one of the key players in the human innate immune system (HIIS). In the event of an insult where the body is exposed to inflammation triggering moieties (ITMs), neutrophils are mobilized towards the site of insult and antagonize the inflammation. If the inflammation is cleared, neutrophils go into a programmed death called apoptosis. Read More

View Article and Full-Text PDF
December 2019

Autologous stem cell transplantation is safe in selected elderly multiple myeloma patients.

Eur J Haematol 2020 Feb 12;104(2):138-144. Epub 2019 Dec 12.

Cellular Therapy and Transfusion Medicine Unit, Careggi University Hospital, Florence, Italy.

Objectives: ASCT is currently the "gold standard" first-line treatment for multiple myeloma patients younger than 65 years old, and limited data on efficacy and safety in older patients are available.

Methods: We retrospectively analyzed a cohort of 83 newly diagnosed multiple myeloma patients aged 65 or older. All patients were evaluated for fitness at diagnosis and after bortezomib-based induction treatment. Read More

View Article and Full-Text PDF
February 2020

Clozapine and desmethylclozapine: correlation with neutrophils and leucocytes counting in Mexican patients with schizophrenia.

BMC Psychiatry 2019 10 9;19(1):295. Epub 2019 Oct 9.

Departamento de Neuroquímica, Instituto Nacional de Neurología "Dr Manuel Velasco Suarez", Ave. Insurgentes Sur No. 3877, 14269, Ciudad de México, D.F, México.

Purpose: The aim of present study is to measure plasma clozapine (CLZ) and N-desmethyl clozapine (DMC) as biomarkers to correlate drug concentrations with the appearance of preclinical adverse hematic effects.

Methods: A high-performance liquid chromatographic method, using a diode-array (ultraviolet) detector, was validated to obtain reliable concentrations of CLZ and DMC, its main metabolite, in plasma of 41 schizophrenic patients taking CLZ. Blood neutrophils and leucocytes counting were concurrently assessed as a proxy to subclinical adverse reactions. Read More

View Article and Full-Text PDF
October 2019

Ibrutinib induces multiple functional defects in the neutrophil response against .

Haematologica 2020 31;105(2):478-489. Epub 2020 Jan 31.

Sorbonne Université, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, Cimi-Paris, Paris

The Bruton tyrosine kinase inhibitor ibrutinib has become a leading therapy against chronic lymphoid leukemia. Recently, ibrutinib has been associated with the occurrence of invasive fungal infections, in particular invasive aspergillosis. The mechanisms underlying the increased susceptibility to fungal infections associated with exposure to ibrutinib are currently unknown. Read More

View Article and Full-Text PDF
January 2020

NEC is likely a NETs dependent process and markers of NETosis are predictive of NEC in mice and humans.

Sci Rep 2018 08 22;8(1):12612. Epub 2018 Aug 22.

Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany.

Necrotizing enterocolitis (NEC) is one of the most devastating diseases affecting premature and mature infants. It is hypothesized that NEC is the result of neutrophils' active role in hyperinflammation after bacterial gut colonization, through their nuclear DNA release and formation of neutrophil extracellular traps (NETs) to combat pathogens. The aim of this study was to evaluate the importance of NETs in NEC pathogenesis, as well as to identify and validate markers of NETosis to predict NEC. Read More

View Article and Full-Text PDF

cfDNA correlates with endothelial damage after cardiac surgery with prolonged cardiopulmonary bypass and amplifies NETosis in an intracellular TLR9-independent manner.

Sci Rep 2017 12 12;7(1):17421. Epub 2017 Dec 12.

Department of Cardiothoracic Surgery, Heart Center of the University of Cologne, Cologne, Germany.

Cardiopulmonary bypass (CPB) provokes inflammation culminating in organ dysfunction and increased mortality. Recently, neutrophil extracellular traps (NETs) have been found to be involved in a variety of cardiovascular diseases promoting tissue and organ injury. Here, we aimed to elaborate the proinflammatory potential of circulating cell-free (cf)DNA in patients undergoing cardiac surgery with CPB. Read More

View Article and Full-Text PDF
December 2017

Cholecystokinin protects mouse liver against ischemia and reperfusion injury.

Int Immunopharmacol 2017 Jul 15;48:180-186. Epub 2017 May 15.

Department of Anesthesiology and Critical Care, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China. Electronic address:

Background: Cholecystokinin (CCK), as a gastrointestinal hormone, has an important protective role against sepsis or LPS-induced endotoxic shock. We aim to address the role of CCK in hepatic ischemia followed by reperfusion (I/R) injury.

Materials And Methods: A murine model of 60min partial hepatic ischemia followed by 6h of reperfusion was used in this study. Read More

View Article and Full-Text PDF

Dataset on granulopoiesis- and lymphopoiesis-stimulating cytokine levels in insulin secretagogue users with incident breast cancer.

Data Brief 2017 Apr 20;11:277-283. Epub 2017 Feb 20.

State University of New York at Buffalo, Department of Pharmacy Practice, NYS Center of Excellence in Bioinformatics and Life Sciences, 701 Ellicott Street, Buffalo, NY 14203, United States; Roswell Park Cancer Institute, Dept. of Pharmacy Services, Elm & Carlton Streets, Buffalo, NY 14263, United States.

GM-CSF and G-CSF are widely used for their benefit in reducing chemotherapy-associated neutropenia. However, whether GM- or G-CSF administration could have tumorigenic or pro-metastatic effects or whether insulin resistance could negatively impact such effects is not known. Their ability to stimulate monocyte production at the same time with the highly sought after neutrophils' production, enables an enhanced potential for activation of tumor-associated macrophages. Read More

View Article and Full-Text PDF

Chemical characterization and protective effect of the Bactris setosa Mart. fruit against oxidative/nitrosative stress.

Food Chem 2017 Apr 30;220:427-437. Epub 2016 Sep 30.

UCIBIO, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. Electronic address:

Bactris setosa Mart. is a Brazilian tree from the palm family (Arecaceae), whose fruits are scientifically underexploited. Here, we report, for the first time, the identification and quantification of phenolic compounds and carotenoids in the pulp, seed, and peel extracts of B. Read More

View Article and Full-Text PDF

Different forms of alpha-1 antitrypsin and neutrophil activation mediated by human anti-PR3 IgG antibodies.

Pharmacol Rep 2016 Dec 5;68(6):1276-1284. Epub 2016 Aug 5.

Department of Internal Medicine, Jagiellonian University Medical College, Kraków, Poland. Electronic address:

Background: One of characteristic findings in granulomatosis with polyangiitis (GPA) is the presence of proteinase-3 (anti-PR3) specific antibodies. These antibodies can cause neutrophil activation, degranulation and generation of reactive oxygen species (ROS). Each of these inflammatory events can be suppressed by circulating alpha-1 antitrypsin (A1AT). Read More

View Article and Full-Text PDF
December 2016


Georgian Med News 2016 Apr(253):98-103

I. Horbachevsky Ternopil State Medical University, Ukraine.

Acute lung injury (ALI) remains a major cause of acute respiratory failure and death of patients. Despite the achievements at the current stage in treatment, morbidity and mortality of ALI remain high. However, a deeper understanding of the pathogenetic links of ALI, identifying of the predictors that positively or negatively influence on the course of the syndrome, the correlation between some pathogenetic mechanisms will improve therapeutic strategies for patients with ALI, which makes the actuality of this study. Read More

View Article and Full-Text PDF

Size-dependent cytotoxicity of silver nanoparticles in human neutrophils assessed by multiple analytical approaches.

Life Sci 2016 Jan 29;145:247-54. Epub 2015 Dec 29.

UCIBIO-REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, 4050-313 Porto, Portugal. Electronic address:

Aims: Silver nanoparticles (AgNPs) have emerged as an important class of nanomaterials with a wide range of industrial and medical applications. The assessment of AgNPs' biological effects in the human organism is therefore essential to evaluate the impact of these nanomaterials in public health and reassure the ratio benefit/risk. In the present study, the effect of polyvinyl pyrrolidone (PVP)-coated AgNPs with distinct sizes (10 and 50nm) on neutrophils' oxidative burst and toxicity were tested. Read More

View Article and Full-Text PDF
January 2016

Heme-related molecules induce rapid production of neutrophil extracellular traps.

Transfusion 2014 Nov 28;54(11):2811-9. Epub 2014 May 28.

Cell Analysis Center, Scientific Affairs, Sysmex Corporation, Kobe, Japan.

Background: Pulmonary endothelial cell damages caused by neutrophil overactivation could result in acute lung injuries including transfusion-related acute lung injury (TRALI). We previously reported that heme-related molecules derived from hemolysis induced the production of reactive oxygen species from neutrophils. Recently, neutrophil extracellular traps (NETs) have been demonstrated to associate with the onset of TRALI. Read More

View Article and Full-Text PDF
November 2014

Nickel induces apoptosis in human neutrophils.

Biometals 2013 Feb 25;26(1):13-21. Epub 2012 Oct 25.

REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313, Porto, Portugal.

Nickel is an ubiquitous transition metal that is industrially applied in many forms, which inevitably leads to a high degree of occupational and environmental exposure. Over-exposure to nickel can produce a variety of adverse effects on human health, including allergy and lung and nasal cancers. In the present study, it is demonstrated, for the first time, that nickel [(Ni(II)] (as a nickel nitrate salt) at concentrations that may be attained in vivo, induces neutrophils' apoptosis by the intrinsic pathway. Read More

View Article and Full-Text PDF
February 2013

[Effects of sodium valproate on neutrophils' oxidative metabolism and oxidant status in children with idiopathic epilepsy].

Zhonghua Er Ke Za Zhi 2011 Oct;49(10):776-81

Department of Pediatric Neurology, Children's Hospital of Fudan University, Shanghai 201102, China.

Objective: To evaluate the influence of VPA treatment on neutrophils' oxidative metabolism and oxidant status in epileptic children.

Method: Twenty-six newly diagnosed epileptic children with idiopathic epilepsy and 30 healthy children were included in the study. The activation rates of neutrophils and stimulation indexes were detected in patients before and 6 months and 12 months after VPA treatment respectively and in all the healthy children by flow cytometry with dihydrorhodamine as fluorochrome. Read More

View Article and Full-Text PDF
October 2011

Effect of lemon verbena supplementation on muscular damage markers, proinflammatory cytokines release and neutrophils' oxidative stress in chronic exercise.

Eur J Appl Physiol 2011 Apr 22;111(4):695-705. Epub 2010 Oct 22.

Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Avda. de la Universidad S/Nº, 03202 Elche, Alicante, Spain.

Intense exercise is directly related to muscular damage and oxidative stress due to excessive reactive oxygen species (ROS) in both, plasma and white blood cells. Nevertheless, exercise-derived ROS are essential to regulate cellular adaptation to exercise. Studies on antioxidant supplements have provided controversial results. Read More

View Article and Full-Text PDF

Ficolin-1 is present in a highly mobilizable subset of human neutrophil granules and associates with the cell surface after stimulation with fMLP.

J Leukoc Biol 2009 Dec 9;86(6):1439-49. Epub 2009 Sep 9.

The Granulocyte Research Laboratory, Department of Hematology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark.

Ficolins are soluble molecules that bind carbohydrate present on the surface of microorganisms and function as recognition molecules in the lectin complement pathway. Three ficolins have been identified in humans: ficolin-1, ficolin-2, and ficolin-3. Ficolin-1 is synthesized in monocytes and type II alveolar epithelial cells. Read More

View Article and Full-Text PDF
December 2009

Effects of 900-MHz radio frequencies on the chemotaxis of human neutrophils in vitro.

IEEE Trans Biomed Eng 2008 Feb;55(2 Pt 1):795-7

Department of Electrical and Computer Engineering, University of Colorado at Boulder, Boulder, CO 80309-0425, USA.

The effects of radio frequency (RF) fields on the ability of human neutrophils to follow concentration gradients of Cyclic Adenosine 3', 5'-Monophosphate (C-AMP) are reported. Blood from healthy adult donors was exposed in vitro to different temperatures and 900-MHz RF field at approximately 0.4 V/m. Read More

View Article and Full-Text PDF
February 2008

Some pathophysiological regularities of formation of digestive diseases in population newly coming to the North.

Alaska Med 2007 ;49(2 Suppl):147-52

Scientific Center of Clinical and Experimental Medicine SB RAMS, Novosibirsk, Russia.

Objectives: The purpose of the work was to reveal some pathophysiological regularities of digestive diseases (DD) development, depending on a period of living in the North.

Study Design: A total of 1,014 workers of industrial enterprises in Mirny-city (Yakutia) have been screened according to the WHO\ CINDI program. DD have been revealed in 321 patients. Read More

View Article and Full-Text PDF
November 2007

Differential surface expression of CD18 and CD44 by neutrophils in bone marrow and spleen contributed to the neutrophilia in thalidomide-treated female B6C3F1 mice.

Toxicol Appl Pharmacol 2007 Feb 22;218(3):227-37. Epub 2006 Nov 22.

Department of Pharmacology and Toxicology, Virginia Commonwealth University, PO Box 980613, Richmond, VA 23298-6013, USA.

Previously, we have reported that thalidomide (Thd) can enhance neutrophil function in female B6C3F1 mice. The present study was intended to evaluate the mechanisms underlying the enhanced neutrophil responses following Thd treatment intraperitoneally (100 mg/kg) for 14 or 28 days. Treatment with Thd increased the numbers of neutrophils in the spleen, peripheral blood, bone marrow, peritoneal cavity and lungs of female B6C3F1 mice when compared to the vehicle control mice. Read More

View Article and Full-Text PDF
February 2007