J Biochem Mol Biol 2001 Nov;34(6):551-558
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, Baltimore, MD 21201, USA.
We extended the measurable time scale of DNA dynamics to submicrosecond using a long-lifetime metal-ligand complex, [Ru(phen)(dppz)] (phen =1,10-phenanthroline, dppz = dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), which displays a mean lifetime near 350 ns. We partially characterized the fluorescence resonance energy transfer (FRET) in calf thymus DNA from RuPD to nile blue (NB) using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. There was a significant overlap of the emission spectrum of the donor RuPD with the absorption spectrum of the acceptor NB. Read More