198,874 results match your criteria three species

DNA polymerase D temporarily connects primase to the CMG-like helicase before interacting with proliferating cell nuclear antigen.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

The eukaryotic replisome is comprised of three family-B DNA polymerases (Polα, δ and ϵ). Polα forms a stable complex with primase to synthesize short RNA-DNA primers, which are subsequently elongated by Polδ and Polϵ in concert with proliferating cell nuclear antigen (PCNA). In some species of archaea, family-D DNA polymerase (PolD) is the only DNA polymerase essential for cell viability, raising the question of how it alone conducts the bulk of DNA synthesis. Read More

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Analysis of Populus glycosyl hydrolase family I members and their potential role in the ABA treatment and drought stress response.

Plant Physiol Biochem 2021 Apr 5;163:178-188. Epub 2021 Apr 5.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China. Electronic address:

Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs) are encoded by a large number of genes and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in the model plant species Arabidopsis thaliana, rice and maize but not in woody plant species, which have important economic and ecological value. In this study, we systematically analyzed Populus BGLUs (PtBGLUs) and demonstrated the involvement of several genes under stress conditions. Read More

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Chronic scrotal hyperthermia induces azoospermia and severe damage to testicular tissue in mice.

Acta Histochem 2021 Apr 10;123(4):151712. Epub 2021 Apr 10.

Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address:

Scrotal hyperthermia leads to altered spermatogenesis due to heat-related oxidative stress. One of the main causes of infertility in men is oxidative stress, which refers to an imbalance in the levels of reactive oxygen species (ROS) and antioxidants. Therefore, this study aimed to evaluate the effects of chronic scrotal hyperthermia on testicular tissue structure, sperm parameters, and oxidative stress in adult mice. Read More

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Damages to biological macromolecules in gonadal subcellular fractions of scallop Chlamys farreri following benzo[a]pyrene exposure: Contribution to inhibiting gonadal development and reducing fertility.

Environ Pollut 2021 Apr 3;283:117084. Epub 2021 Apr 3.

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, PR China.

Benzo[a]pyrene (B[a]P), a representative polycyclic aromatic hydrocarbon (PAH) compound in marine ecosystem, has great potential for chronic toxicity to marine animals. It is becoming increasingly apparent that reproductive system is the major target of B[a]P, but the adverse effects of B[a]P on subcellular fractions in bivalve gonads have not been elucidated. Scallops Chlamys farreri are used as the experimental species since they are sensitive to environmental pollutants. Read More

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Drivers of pro-environmental behaviours among outdoor recreationists: The case of a recreational fishery in Western Canada.

J Environ Manage 2021 Apr 10;289:112366. Epub 2021 Apr 10.

School of Sociological and Anthropological Studies, University of Ottawa, Ottawa, ON, Canada.

Pro-environmental behaviours (PEBs) are important for mitigation and restoration efforts in the Anthropocene. As recreationists are motivated to engage in leisure activities to increase their own personal wellbeing, we submit that threats to wellbeing (an egocentric motivator) predict engagement in PEBs amongst recreationists. We also predict that differences in experiences across groups of recreationists leads to differences in PEB engagement. Read More

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Unraveling interfacial properties of organic-coated marine aerosol with lipase incorporation.

Sci Total Environ 2021 Apr 5;782:146893. Epub 2021 Apr 5.

Environment Research Institute, Shandong University, Binhai Road 72, Qingdao 266237, China. Electronic address:

Marine aerosols are believed to have an organic surface coating on which fatty acids act as an important component due to their high surface activity. In addition, various kinds of enzyme species are abundantly found in seawater, some of which have been identified to exist in marine aerosols. Herein, from the perspective of marine aerosol interface simulation, we investigate the effect of Burkholderia cepacia lipase on the surface properties of stearic acid (SA) monolayer at the air-water interface by using surface-sensitive techniques of Langmuir trough and Infrared reflection-absorption spectroscopy (IRRAS). Read More

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Land cover alteration shifts ecological assembly processes in floodplain lakes: Consequences for fish community dynamics.

Sci Total Environ 2021 Mar 25;782:146724. Epub 2021 Mar 25.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China; Science Division, NSW Department of Planning, Industry and Environment, Sydney 2124, Australia. Electronic address:

Habitat degradation is expected to alter community structure and consequently, ecosystem functions including the maintenance of biodiversity. Understanding the underlying abiotic and biotic assembly mechanisms controlling temporal and spatial community structure and patterns is a central issue in biodiversity conservation. In this study, using monthly time series of fish abundance data collected over a three-year period, we compared the temporal community dynamics in natural habitats and poplar plantations in one of the largest river-lake floodplain ecosystems in China, the Dongting Lake. Read More

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High sensitivity of invertebrate detritivores from tropical streams to different pesticides.

Ecotoxicol Environ Saf 2021 Apr 10;216:112226. Epub 2021 Apr 10.

Department of Plant Biology and Ecology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Leioa, Spain; IKERBASQUE, Bilbao, Spain.

Freshwater organisms are often sensitive to pesticides, but their sensitivity varies across different taxa and with pesticide type and action mode, as shown by multiple acute toxicity tests. Such variability hampers predictions about how freshwater ecosystems may be altered by pesticide toxicity, which is especially critical for understudied areas of the world such as the tropics. Furthermore, there is little information about the sensitivity of some organisms that are key components of stream food webs; this is the case of litter-feeding detritivorous invertebrates, which contribute to the fundamental process of litter decomposition. Read More

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Highly divergent neuropeptide - non-coding RNA regulatory networks underpin variant host-finding behaviours in Steinernema species infective juveniles.

Int J Parasitol 2021 Apr 10. Epub 2021 Apr 10.

School of Biological Sciences, Queen's University Belfast, Belfast, UK; Agri-Food and Biosciences Institute (AFBI), Belfast, UK.

We conducted a transcriptomic and small RNA analysis of infective juveniles (IJs) from three behaviourally distinct Steinernema species. Substantial variation was found in the expression of shared gene orthologues, revealing gene expression signatures that correlate with behavioural states. Ninety-seven percent of predicted microRNAs are novel to each species. Read More

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The emergence of a complex pore-canal system in the dermal skeleton of Tremataspis (Osteostraci).

J Morphol 2021 Apr 13. Epub 2021 Apr 13.

Department of Organismal Biology, Uppsala University, Uppsala, Sweden.

Thyestiids are a group of osteostracans (sister-group to jawed vertebrates) ranging in time from the early Silurian to Middle Devonian. Tremataspis is unique among thyestiids in having a continuous mesodentine and enameloid cover on its dermal elements, and an embedded pore-canal system divided into lower and upper parts by a perforated septum. The origin of this upper mesh canal system and its potential homology to similar canal systems of other osteostracans has remained a matter of debate. Read More

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Differential proteome profile of gill and spleen in three pathogen-infected Paralichthys olivaceus.

Genes Genomics 2021 Apr 13. Epub 2021 Apr 13.

Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, Republic of Korea.

Background: Olive flounder (Paralichthys olivaceus) is one of the major cultured fish species in Asia including Korea. However, the mass mortality of olive flounder caused by various pathogens leads to huge economic loss. The pathogens that lead to fish mortality include parasites, bacteria, and viruses that can cause various kinds of diseases. Read More

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The temporal development of plant-soil feedback is contingent on competition and nutrient availability contexts.

Petr Dostál

Oecologia 2021 Apr 13. Epub 2021 Apr 13.

Institute of Botany, The Czech Academy of Sciences, 252 43, Průhonice, Czech Republic.

Strength and direction of plant-soil feedback (PSF), the reciprocal interactions between plants and soil, can change over time and have distinct effects on different life stages. PSF and its temporal development can also be modified by external biotic and abiotic factors such as competition and resource availability, yet most PSF research is conducted in simple experimental settings without considering temporal changes. Here I have studied the effect of different competitive settings (intraspecific, interspecific, and no competition) and nutrient addition on the magnitude and direction of biomass-based PSF (performance in conspecific relative to heterospecific inoculum) across 46 grassland species, estimated at the 4th, 10th, and 13th month of the response phase. Read More

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Antibacterial Activity in Iodine-coated Implants Under Conditions of Iodine Loss: Study in a Rat Model Plus In Vitro Analysis.

Clin Orthop Relat Res 2021 Apr 11. Epub 2021 Apr 11.

K. Ueoka, T. Kabata, Y. Kajino, D. Inoue, T. Takagi, T. Ohmori, J. Yoshitani, T. Ueno, Y. Yamamuro, A. Taninaka, H. Tsuchiya, Department of Orthopedic Surgery, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.

Background: We developed iodine-coated titanium implants to suppress microbial activity and prevent periprosthetic joint infection (PJI); their efficacy was demonstrated in animal and in vitro models. The iodine content in iodine-coated implants naturally decreases in vivo. However, to our knowledge, the effect of reduced iodine content on the implant's antimicrobial activity has not been evaluated to date. Read More

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Cryptic Diversity in the Terminal Portion of the Chromosomes of the Dogtooth Characins, Family Cynodontidae (Ostariophysi: Characiformes).

Zebrafish 2021 Apr 12. Epub 2021 Apr 12.

Institute for Natural and Life Sciences, Federal University of Latin American Integration, Foz do Iguaçu, Brazil.

The chromosomes of the dogtooth characins, fish species of the family Cynodontidae, have only a relatively small amount of heterochromatin, including the terminal portion. Curiously, in the cynodontid , the terminal portion is rich in repetitive DNAs, including transposable retroelements and microsatellite sequences. Given this, this study investigated the composition of the terminal portion of the chromosomes of two cynodontid species ( and ), to compile a database for the evaluation of all three cynodontid genera, and in particular, verify the possible tendency for the accumulation of repetitive DNAs in the terminal portion of the chromosomes of , , and The 1, 3, and 6 transposable retroelements and the (CA) (GA), (GATA), (GACA), (CAT), and (CAC) microsatellite motifs are found primarily in the terminal portion of the chromosomes of the species analyzed in this study, except , which has no evidence of the presence of or through the fluorescent hybridization technique. Read More

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Evaluation of Interfacial pH Using Surface Forces Apparatus Fluorescence Spectroscopy.

Langmuir 2021 Apr 13. Epub 2021 Apr 13.

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan.

The fluorescence spectrum measurement of a fluorescence pH probe, C. SNARF-4F, was performed for monitoring the interfacial pH of aqueous electrolytes between mica or silica surfaces while varying the surface separation () using surface force apparatus (SFA) fluorescence spectroscopy. The pH of the aqueous CsCl between mica exponentially decreased with decreasing . Read More

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Survey of anthelmintic resistance in a Romanian horse stud using three different methods.

Pol J Vet Sci 2021 Mar;24(1):145-149

Banat University of Agricultural Sciences and Veterinary Medicineof Timişoara, Faculty of Veterinary Medicine, 300645, Calea Aradului no. 119, Timişoara, Romania.

Resistance of cyathostomins to benzimidazole (BZ) anthelmintics is widespread in horses in many parts of the world. This study compared three methods for the determination of benzimidazole resistance of Cyathostominae in 18 horses from a stud farm in Romania. The horses were treated with Fenbendazole. Read More

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Plastic Variation in the Phyletic Lineages of Cactophilic Drosophila meridionalis and Relation to Hosts as Potential for Diversification.

Neotrop Entomol 2021 Apr 12. Epub 2021 Apr 12.

Pós-Graduação em Genética - Faculdade de Medicina de Ribeirão Preto, Univ de São Paulo, Ribeirão Preto, Brazil.

The insect/plant interaction is known to be a trigger for diversification and even speciation. Experimental analyses on fitness traits and phenotypic variation using alternative host sites have been performed to understand the process of diversification relative to insect/plant interactions. For cactophilic species of Drosophila, the speciation process is considered an adaptive radiation in response to the exploration of species of the Cactaceae as breeding and feeding sites. Read More

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A novel gene variant (c.857A>G; p.Tyr286Cys) in an extended family with non‑syndromic deafness 2A.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China.

Deafness is one of the most common sensory disorders found in humans; notably, >60% of all cases of deafness have been attributed to genetic factors. Variants in potassium voltage‑gated channel subfamily Q member 4 () are etiologically linked to a type of progressive hearing loss, deafness non‑syndromic autosomal dominant 2A (DFNA2A). In the present study, whole‑exome sequencing (WES) was performed on three members of a five‑generation Chinese family with 46 members with hearing loss. Read More

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Inferring number of populations and changes in connectivity under the n-island model.

Heredity (Edinb) 2021 Apr 12. Epub 2021 Apr 12.

Instituto Gulbenkian de Ciência, Oeiras, Portugal.

Inferring the demographic history of species is one of the greatest challenges in populations genetics. This history is often represented as a history of size changes, ignoring population structure. Alternatively, when structure is assumed, it is defined a priori as a population tree and not inferred. Read More

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Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and retinitis pigmentosa patients.

Eye (Lond) 2021 Apr 12. Epub 2021 Apr 12.

Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou, Guangdong, China.

Objectives: To delineate the disease-causing mutations of the Stargardt disease-related genes in Chinese patients diagnosed with Stargardt disease or retinitis pigmentosa (RP) by whole exome sequencing analysis.

Methods: A total of 123 sporadic RP or Stargardt disease patients and 2 Stargardt disease families were recruited. All sporadic patients and the probands of the families were subjected to whole exome sequencing analysis. Read More

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Micafungin population PK analysis in plasma and peritoneal fluid in septic patients with intra-abdominal infections: a prospective cohort study.

Antimicrob Agents Chemother 2021 Apr 12. Epub 2021 Apr 12.

Service des Réanimations, Pôle Anesthésie Réanimation Douleur Urgence, CHU Nîmes, Nîmes, France.

To describe the pharmacokinetics (PK) of micafungin in plasma and peritoneal fluid in septic patients with intra-abdominal infections. Twelve patients with secondary peritonitis in septic shock receiving 100 mg micafungin once daily were included. Total micafungin plasma and peritoneal fluid were subject to a population pharmacokinetic analysis using Pmetrics®. Read More

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Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis.

Parasit Vectors 2021 Apr 12;14(1):198. Epub 2021 Apr 12.

Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez S/N, 38203, La Laguna, Tenerife, Spain.

Background: The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. Read More

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Niche partitioning in the Rimicaris exoculata holobiont: the case of the first symbiotic Zetaproteobacteria.

Microbiome 2021 Apr 12;9(1):87. Epub 2021 Apr 12.

Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, 29280, Plouzané, France.

Background: Free-living and symbiotic chemosynthetic microbial communities support primary production and higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata, which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax. The dominant bacteria present are from the taxonomic groups Campylobacteria, Desulfobulbia (formerly Deltaproteobacteria), Alphaproteobacteria, Gammaproteobacteria, and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria. Read More

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Three in one: evolution of viviparity, coenocytic placenta and polyembryony in cyclostome bryozoans.

BMC Ecol Evol 2021 Apr 12;21(1):54. Epub 2021 Apr 12.

Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, Universitetskaja nab. 7/9, 199034, Saint Petersburg, Russia.

Background: Placentation has evolved multiple times among both chordates and invertebrates. Although they are structurally less complex, invertebrate placentae are much more diverse in their origin, development and position. Aquatic colonial suspension-feeders from the phylum Bryozoa acquired placental analogues multiple times, representing an outstanding example of their structural diversity and evolution. Read More

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Advanced materials for immobilization of purple phototrophic bacteria in bioremediation of oil-polluted wastewater.

Chemosphere 2021 Apr 2;278:130464. Epub 2021 Apr 2.

Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, Malaysia. Electronic address:

Oil pollution which results from industrial activities, especially oil and gas industry, has become a serious issue. Cinder beats (CB), coconut fiber (CF) and polyurethane foam (PUF) are promising immobilization carriers for crude oil biodegradation because they are inexpensive, nontoxic, and non-polluting. The present investigation was aimed to evaluate this advanced technology and compare the efficiency of these immobilization carriers on supporting purple phototrophic bacterial (PPB) strains in hydrocarbon biodegradation of crude oil contaminated seawater. Read More

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Untargeted GC/MS-based approach for identification of anti-inflammatory alkaloids from Hippeastrum elegans (Amaryllidaceae) using a human neutrophil model.

J Pharm Biomed Anal 2021 Apr 3;199:114061. Epub 2021 Apr 3.

Centro de Estudos Farmacêuticos e Cosméticos (CEFAC), Faculdade de Farmácia, Odontologia e Enfermagem, Departamento de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brazil. Electronic address:

Hippeastrum elegans is an Amaryllidaceae species producing alkaloids with pharmaceutical potential including lycorine and galanthamine. Herein, we developed a non-targeted metabolomic study associated to chemometrics and biological evaluations to identify the H. elegans constituents that were able to reduce the human neutrophils proinflammatory mechanisms. Read More

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The Indian Ocean Dipole and El Niño Southern Oscillation as major drivers of coral cover on shallow reefs in the Andaman Sea.

Glob Chang Biol 2021 Apr 12. Epub 2021 Apr 12.

Marine and Coastal Resources Research Center (Upper Eastern Gulf of Thailand), 58/5 Moo 8 Songklong Bangpakong, chachoengsao, Thailand, 24130.

Shallow reefs are a major feature of coral assemblages in the Andaman Sea. At Phuket, Thailand sheltered reefs are dominated by massive corals, together with an increasing abundance of branching species during favourable growth conditions. The growth of coral on these reefs is moderated by long-term increases in sea temperature and relative sea level but fluctuating decadal/intradecadal climate processes of El Niño Southern Oscillation (ENSO) and Indian Ocean Dipole (IOD), which modulate sea level and temperature, are the main drivers of coral cover. Read More

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Energetic constraints imposed on trophic interaction strengths enhance resilience in empirical and model food webs.

J Anim Ecol 2021 Apr 12. Epub 2021 Apr 12.

Institute of Biochemistry and Biology, University of Potsdam, 14469, Potsdam, Germany.

Food web stability and resilience are at the heart of understanding the structure and functioning of ecosystems. Previous studies show that models of empirical food webs are substantially more stable than random ones, due to a few strong interactions embedded in a majority of weak interactions. Analyses of trophic interaction loops show that in empirical food webs the patterns in the interaction strengths prevent the occurrence of destabilizing heavy loops and thereby enhances resilience. Read More

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Integral Multielement Signals by DNA-Programmed UCNP-AuNP Nanosatellite Assemblies for Ultrasensitive ICP-MS Detection of Exosomal Proteins and Cancer Identification.

Anal Chem 2021 Apr 12. Epub 2021 Apr 12.

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, P.O. Box 332, Shenyang 110819, China.

Exosomes are expected to be used as cancer biomarkers because they carry a variety of cancer-related proteins inherited from parental cells. However, it is still challenging to develop a sensitive, robust, and high-throughput technique for simultaneous detection of exosomal proteins. Herein, three aptamers specific to cancer-associated proteins (CD63, EpCAM, and HER2) are selected to connect gold nanoparticles (AuNPs) as core with three different elements (Y, Eu, and Tb) doped up-conversion nanoparticles (UCNPs) as satellites, thereby forming three nanosatellite assemblies. Read More

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Amidinate Supporting Ligands Influence Molecularity in Formation of Uranium Nitrides.

Inorg Chem 2021 Apr 12. Epub 2021 Apr 12.

Department of Chemistry, University of California, Berkeley, California 94720, United States.

Uranium nitride complexes are attractive targets for chemists as molecular models for the bonding, reactivity, and magnetic properties of next-generation nuclear fuels, but these molecules are uncommon and can be difficult to isolate due to their high reactivity. Here, we describe the synthesis of three new multinuclear uranium nitride complexes, [U(BCMA)](μ-N)(μ-κ:κ-BCMA) (), [(U(BIMA))(μ-N)(μ-NPr)(K(μ-η:η-CHCHNPr)] (), and [U(BIMA)](μ-N)(μ-κ:κ-BIMA) () (BCMA = ,-bis(cyclohexyl)methylamidinate, BIMA = ,-bis(-propyl)methylamidinate), from U(III) and U(IV) amidinate precursors. By varying the amidinate ligand substituents and azide source, we were able to influence the composition and size of these nitride complexes. Read More

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