12,604 results match your criteria three copies

[Prevalence of transmitted drug resistance in HIV-infected treatment-naive patients in Chile].

Rev Med Chil 2020 Nov;148(11):1550-1557

Laboratorio de Medicina Molecular, Hospital Clínico, Universidad de Chile, Santiago, Chile.

Background: Transmitted drug resistance (TDR) occurs in patients with HIV infection who are not exposed to antiretroviral drugs but who are infected with a virus with mutations associated with resistance.

Aim: To determine the prevalence of TDR and characterize HIV reverse transcriptase and protease mutation patterns.

Material And Methods: HIV infected antiretroviral treatment-naive patients treated in three centers between 2014 and 2018 were studied. Read More

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November 2020

Technical note: multi-alleles at the DYS385ab locus with high frequency in a Han Chinese population from southwestern China.

Int J Legal Med 2021 Apr 12. Epub 2021 Apr 12.

Laboratory of Forensic DNA, the Judicial Authentication Center, Southwest Medical University, Luzhou, 646000, Sichuan, China.

Y-chromosome short tandem repeat (Y-STR) markers have been widely used in forensic applications and usually show monoallelic or diallelic genotypic patterns at certain double-copied loci. In this study, we have found 13 samples among 703 males with multi-alleles at the DYS385ab locus, including one with five mutant alleles, nine with four, and three with three. The frequency of abnormal DYS385ab genotypes was 1. Read More

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Structural basis of drug recognition by the multidrug transporter ABCG2.

J Mol Biol 2021 Apr 7:166980. Epub 2021 Apr 7.

Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland. Electronic address:

ABCG2 is an ATP-binding cassette (ABC) transporter whose function affects the pharmacokinetics of drugs and contributes to multidrug resistance of cancer cells. While its interaction with the endogenous substrate estrone-3-sulfate (ES) has been elucidated at a structural level, the recognition and recruitment of exogenous compounds is not understood at sufficiently high resolution. Here we present three cryo-EM structures of nanodisc-reconstituted, human ABCG2 bound to anticancer drugs tariquidar, topotecan and mitoxantrone. Read More

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Development of multiplex real-time PCR assays for differential detection of capripoxvirus, parapoxvirus, and foot-and-mouth disease virus.

Transbound Emerg Dis 2021 Apr 10. Epub 2021 Apr 10.

Foreign Animal Disease Diagnostic Laboratory, NVSL, APHIS, USDA, Plum Island Animal Disease Center, Orient, NY, 11957, United States.

This study reports the development of multiplex real-time PCR assays for differential detection of capripoxvirus (CaPV), parapoxvirus (PaPV), and foot-and-mouth disease virus (FMDV) in sheep, goats and cattle. Three multiplex assays were developed, a capripox (CaP) rule-out assay for simultaneous detection and differentiation of CaPV and PaPV, a FMD rule-out assay for simultaneous detection and differentiation of FMDV and PaPV, and a FMD/CaP rule-out assay for simultaneous detection and differentiation of CaPV, PaPV and FMDV. All multiplex assays included β-actin gene ACTB as an internal positive control to monitor PCR inhibition and accuracy of nucleic acid extractions. Read More

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Development of a set of three real-time loop-mediated isothermal amplification (LAMP) assays for detection of Bacillus anthracis, the causative agent of anthrax.

Folia Microbiol (Praha) 2021 Apr 9. Epub 2021 Apr 9.

Bioprocess Technology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior-474 002, India.

Bacillus anthracis, the causative agent of anthrax is a Gram-positive, non-motile, spore forming bacterium. Its spores can persist in soil and water for years and can also be aerosolized. A rapid, sensitive and specific method to detect B. Read More

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The reference genome and transcriptome of the limestone langur, Trachypithecus leucocephalus, reveal expansion of genes related to alkali tolerance.

BMC Biol 2021 Apr 8;19(1):67. Epub 2021 Apr 8.

Department of Biochemistry and Molecular Biology, School of Pre-Clinical Medicine, Guangxi Medical University, Nanning, Guangxi, 530021, China.

Background: Trachypithecus leucocephalus, the white-headed langur, is a critically endangered primate that is endemic to the karst mountains in the southern Guangxi province of China. Studying the genomic and transcriptomic mechanisms underlying its local adaptation could help explain its persistence within a highly specialized ecological niche.

Results: In this study, we used PacBio sequencing and optical assembly and Hi-C analysis to create a high-quality de novo assembly of the T. Read More

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Etravirine in treatment-experienced HIV-1 infected children 1-<6 years of age.

AIDS 2021 Apr 6. Epub 2021 Apr 6.

University of Colorado Anschutz Medical Campus, Aurora, CO Children's Hospital of Philadelphia, Philadelphia, PA Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, NY Frontier Science and Technology, Buffalo, NY Harvard School of Public Health, Boston, MA Shandukani Research Centre, Johannesburg, South Africa FHI 360, Durham, NC ColumbusUSA Technologies contract at NIAID, Maternal, Adolescent & Pediatric Research Branch, Rockville, MD Eunice Kennedy Shriver National Institute of Child Health & Human Development, Bethesda, MD IMPAACT Laboratory Center, University of California Los Angeles, Los Angeles, CA Preventive Medicine Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Janssen, Research and Development, Beerse, Belgium.

Objective: To describe the pharmacokinetics (PK), safety, and efficacy of etravirine (ETR) in HIV-infected children 1 to < 6 yrs.

Design: Phase I/II, open label, multicenter, dose-finding study.

Methods: Antiretroviral (ARV)-experienced children in two age cohorts (I: 2-<6 yr; II: 1-<2 yr) received weight-based ETR, swallowed whole or dispersed in liquid, with optimized ARV therapy including a ritonavir-boosted protease inhibitor. Read More

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Morphological and molecular analyses of a population with high intragenomic rRNA polymorphism.

J Nematol 2020 6;52. Epub 2020 Nov 6.

Ningbo Customs Technical Centre (Ningbo Inspection and Quarantine Science Technology Academy), 8 Huikang Road, Ningbo, 315100, Zhejiang, P.R. China.

Root-knot nematode, can cause damage in trees, shrubs, and herbaceous plants, and was placed on the EPPO Alert List in 2014. In the present study, we report a population isolated from Japanese maple. The recovered population is described by detailed morphological and molecular approaches. Read More

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November 2020

Developing Familiarity in a New Duo: Rehearsal Talk and Performance Cues.

Front Psychol 2021 16;12:590987. Epub 2021 Mar 16.

Assistant Provost, Bond University, Robina, QLD, Australia.

Social and cognitive processes underlying individual classical musicians' and duo performers' preparation for performance have been explored using longitudinal case studies. Social processes can be inferred from and recent studies have focused on its content and nature. Cognitive processes can be inferred from score annotations representing musicians' thoughts while practicing, rehearsing (), and playing or singing from memory (). Read More

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Fine-Scale Temporal Dynamics of SARS-CoV-2 RNA Abundance in Wastewater during A COVID-19 Lockdown.

Water Res 2021 Mar 29;197:117093. Epub 2021 Mar 29.

Department of Civil and Environmental Engineering, University of Hawaii at Manoa, Honolulu, HI 96822. Electronic address:

Wastewater is a pooled sampling instrument that may provide rapid and even early disease signals in the surveillance of COVID-19 disease at the community level, yet the fine-scale temporal dynamics of SARS-CoV-2 RNA in wastewater remains poorly understood. This study tracked the daily dynamics of SARS-CoV-2 RNA in the wastewater from two wastewater treatment plants (WWTPs) in Honolulu during a rapidly expanding COVID-19 outbreak and a responding four-week lockdown that resulted in a rapid decrease of daily clinical COVID-19 new cases. The wastewater SARS-CoV-2 RNA concentration from both WWTPs, as measured by three quantification assays (N1, N2, and E), exhibited both significant inter-day fluctuations (10-10 gene copies or GC/L in wastewater liquid fractions, or 10-10 GC/g in solid fractions) and an overall downward trend over the lockdown period. Read More

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CO and N O emissions and microbial community structure from fields that include salt-affected soils.

J Environ Qual 2021 Apr 7. Epub 2021 Apr 7.

Distinguished Professor, Agronomy, Horticulture, and Plant Science, South Dakota State University, Brookings, SD.

Although salinity and sodicity are world-wide problems, information on greenhouse gas emissions from agricultural salt-affected soils is scarce. The CO -C and N O-N emissions were quantified from three zones intertwined within a single Northern Great Plains USA field, a highly productive zone (EC = 0.4 dS m ; SAR = 1. Read More

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Lumipulse G SARS-CoV-2 Ag assay evaluation using clinical samples from different testing groups.

Clin Chem Lab Med 2021 Apr 7. Epub 2021 Apr 7.

Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.

Objectives: Compared to RT-PCR, lower performance of antigen detection assays, including the Lumipulse G SARS-CoV-2 Ag assay, may depend on specific testing scenarios.

Methods: We tested 594 nasopharyngeal swab samples from individuals with COVID-19 (RT-PCR cycle threshold [Ct] values ≤ 40) or non-COVID-19 (Ct values > 40) diagnoses. RT-PCR positive samples were assigned to diagnostic, screening, or monitoring groups of testing. Read More

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Utilization of Standard Method Performance Requirements for the Detection of Coxiella burnetii in Environmental Samples.

J AOAC Int 2020 Jul 23. Epub 2020 Jul 23.

Wadsworth Center, New York State Department of Health, 120 New Scotland Ave, Albany, NY 12208, USA.

Background: Coxiella burnetii, the causative agent of Q fever, is a long-standing public health problem. Infected animals shed the organism, resulting in aerosol transmission to humans. This organism can potentially be used as a bioterrorism weapon and is on the Department of Health and Human Service Select Agent List. Read More

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Obturator Oblique and Pubic Ramus Inlet Views Can Better Guide the Insertion of an Anterior Column Acetabular Screw.

Orthop Surg 2021 Apr 5. Epub 2021 Apr 5.

Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: The objective of the present paper was to investigate the value of obturator oblique and pubic ramus inlet views in guiding anterior column acetabular screw insertion.

Methods: We collected pelvic CT scans at the diagnostic imaging center of our hospital between 2017 and 2019. Virtual three-dimensional (3D) models of the pelvis were created based on the CT scans. Read More

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Detecting SARS-CoV-2 in the Breath of COVID-19 Patients.

Front Med (Lausanne) 2021 17;8:604392. Epub 2021 Mar 17.

Commune of Scientific Engineers, Institute of Physics, Chinese Academy of Sciences, Beijing, China.

In the COVID-19 outbreak year 2020, a consensus was reached on the fact that SARS-CoV-2 spreads through aerosols. However, finding an efficient method to detect viruses in aerosols to monitor the risk of similar infections and enact effective control remains a great challenge. Our study aimed to build a swirling aerosol collection (SAC) device to collect viral particles in exhaled breath and subsequently detect SARS-CoV-2 using reverse transcription polymerase chain reaction (RT-PCR). Read More

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Development and performance evaluation of the first in-house multiplex rRT-PCR assay in Bangladesh for highly sensitive detection of SARS-CoV-2.

J Virol Methods 2021 Apr 1;293:114147. Epub 2021 Apr 1.

Molecular Division, OMC Healthcare (Pvt.) Ltd, Rupnagar, Dhaka, 1216, Bangladesh. Electronic address:

Background: The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic is posing a great threat to global health and economy. Due to the lack of broad diagnostic setup, consistent reagent supply lines, and access to laboratory instruments and equipment, it is undoubtedly an enormous burden for developing countries to face the crisis.

Objectives: To develop a cost-effective, reliable and sensitive multiplex assay for SARS-CoV-2 screening which would expand the testing capacities of a developing and low-income country like Bangladesh. Read More

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Animal models for SARS-Cov2/Covid19 research-A commentary.

Biochem Pharmacol 2021 Apr 2;188:114543. Epub 2021 Apr 2.

Synbal, Inc., 10210 Campus Point DR. #150, San Diego, CA 92121, United States. Electronic address:

Introduction: There is an urgent need for new animal models of SARS CoV-2 infection to improve research and drug development. This brief commentary examines the deficits of current models and proposes several improved alternates. The existing single transgene mouse models poorly mimic the clinical features of COVID-19; those strains get a milder disease than human COVID-19 disease. Read More

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Efficacy and safety of dolutegravir with emtricitabine and tenofovir alafenamide fumarate or tenofovir disoproxil fumarate, and efavirenz, emtricitabine, and tenofovir disoproxil fumarate HIV antiretroviral therapy regimens started in pregnancy (IMPAACT 2010/VESTED): a multicentre, open-label, randomised, controlled, phase 3 trial.

Lancet 2021 Apr;397(10281):1276-1292

David Geffen School of Medicine, Division of Infectious Diseases, University of California, Los Angeles, Los Angeles, CA, USA.

Background: Antiretroviral therapy (ART) during pregnancy is important for both maternal health and prevention of perinatal HIV-1 transmission; however adequate data on the safety and efficacy of different ART regimens that are likely to be used by pregnant women are scarce. In this trial we compared the safety and efficacy of three antiretroviral regimens started in pregnancy: dolutegravir, emtricitabine, and tenofovir alafenamide fumarate; dolutegravir, emtricitabine, and tenofovir disoproxil fumarate; and efavirenz, emtricitabine, and tenofovir disoproxil fumarate.

Methods: This multicentre, open-label, randomised controlled, phase 3 trial was done at 22 clinical research sites in nine countries (Botswana, Brazil, India, South Africa, Tanzania, Thailand, Uganda, the USA, and Zimbabwe). Read More

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The evolutionary fate of rpl32 and rps16 losses in the Euphorbia schimperi (Euphorbiaceae) plastome.

Sci Rep 2021 Apr 2;11(1):7466. Epub 2021 Apr 2.

Department of Integrative Biology, University of Texas at Austin, Austin, TX, 78712, USA.

Gene transfers from mitochondria and plastids to the nucleus are an important process in the evolution of the eukaryotic cell. Plastid (pt) gene losses have been documented in multiple angiosperm lineages and are often associated with functional transfers to the nucleus or substitutions by duplicated nuclear genes targeted to both the plastid and mitochondrion. The plastid genome sequence of Euphorbia schimperi was assembled and three major genomic changes were detected, the complete loss of rpl32 and pseudogenization of rps16 and infA. Read More

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The Abundance and Diversity of Fungi in a Hypersaline Microbial Mat from Guerrero Negro, Baja California, México.

J Fungi (Basel) 2021 Mar 12;7(3). Epub 2021 Mar 12.

Exobiology Branch, NASA Ames Research Center, Moffett Field, CA 94035, USA.

The abundance and diversity of fungi were evaluated in a hypersaline microbial mat from Guerrero Negro, México, using a combination of quantitative polymerase chain reaction (qPCR) amplification of domain-specific primers, and metagenomic sequencing. Seven different layers were analyzed in the mat (Layers 1-7) at single millimeter resolution (from the surface to 7 mm in depth). The number of copies of the 18S rRNA gene of fungi ranged between 10 and 10 copies per g mat, being two logarithmic units lower than of the 16S rRNA gene of bacteria. Read More

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Identification of Enterotype and Its Effects on Intestinal Butyrate Production in Pigs.

Animals (Basel) 2021 Mar 8;11(3). Epub 2021 Mar 8.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Gut microbiota is thought to play a crucial role in nutrient digestion for pigs, especially in processing indigestible polysaccharides in the diets to produce short-chain fatty acids (SCFAs). However, the link between microbiota community structure and phenotypic performances are poorly understood. In the present study, the fecal samples of 105 Jinhua pigs at 105 days of age were clustered into three enterotypes (ETs, ET1, ET2, and ET3) that are subpopulations of distinct bacterial community composition by using 16S rRNA high throughput sequencing. Read More

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Multi-site Evaluation and Validation of a Sensitive Diagnostic and Screening System for Spinal Muscular Atrophy that Reports SMN1 and SMN2 Copy Number, along with Disease Modifier and Gene Duplication Variants.

J Mol Diagn 2021 Mar 30. Epub 2021 Mar 30.

Asuragen Inc. Austin, Texas.

Spinal muscular atrophy (SMA) is a severe autosomal recessive disease caused by disruptions in the SMN1 gene. The nearly identical SMN2 gene copy number is associated with disease severity. SMN1 duplication markers such as c. Read More

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Analytical sensitivity comparison of 14 conventional and three rapid RT-PCR assays for SARS-CoV-2 detection.

J Virol Methods 2021 Mar 30;293:114144. Epub 2021 Mar 30.

Department of Molecular Biology, Shanghai Centre for Clinical Laboratory, Shanghai, China. Electronic address:

Recent reports have compared the analytical sensitivities of some SARS-CoV-2 RT-PCR assays, but differences in the viral materials used for these evaluations made comprehensive conclusions difficult. We carried out a direct comparison of the analytical sensitivities of 14 conventional and three rapid RT-PCR assays for the detection of SARS-CoV-2. The comparison was performed utilizing a certified reference material for SARS-CoV-2 RNA that was serially two-fold diluted in RNA storage solution. Read More

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Increased expression of Polδ does not alter the canonical replication program .

Wellcome Open Res 2021 26;6:44. Epub 2021 Feb 26.

Genome Damage and Stability Centre, School of Life Sciences, Science Park Road, University of Sussex, Falmer, Brighton, BN1 9RQ, UK.

experiments utilising the reconstituted eukaryotic replisome indicated that the efficiency of the leading strand replication is impaired by a moderate increase in Polδ concentration. It was hypothesised that the slower rate of the leading strand synthesis characteristic for reactions containing two-fold and four-fold increased concentration of Polδ represented a consequence of a relatively rare event, during which Polδ stochastically outcompeted Polε and, in an inefficient manner, temporarily facilitated extension of the leading strand. Inspired by this observation, we aimed to determine whether similarly increased Polδ levels influence replication dynamics using the fission yeast as a model system. Read More

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February 2021

Specific and sensitive, ready-to-use universal fungi detection by visual color using ITS1 loop-mediated isothermal amplification combined hydroxynaphthol blue.

PeerJ 2021 18;9:e11082. Epub 2021 Mar 18.

Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.

Being ubiquitous, fungi are common opportunistic pathogens to humans that can lead to invasive and life-threatening infections in immunocompromised individuals. Eukaryote-resembling cell membrane and filamentous branches make the fungal diagnosis difficult. This study therefore developed a ready-to-use ITS1 loop-mediated isothermal amplification combined with hydroxynaphthol blue (LAMP-HNB) for rapid, sensitive and specific colorimetric detection of universal fungi in all phyla. Read More

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Molecular Evolution and Organization of Ribosomal DNA in the Hawkweed Tribe Hieraciinae (Cichorieae, Asteraceae).

Front Plant Sci 2021 12;12:647375. Epub 2021 Mar 12.

Institute of Botany, Czech Academy of Sciences, Průhonice, Czechia.

Molecular evolution of ribosomal DNA can be highly dynamic. Hundreds to thousands of copies in the genome are subject to concerted evolution, which homogenizes sequence variants to different degrees. If well homogenized, sequences are suitable for phylogeny reconstruction; if not, sequence polymorphism has to be handled appropriately. Read More

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