11,058 results match your criteria three biologically

A Brief Negotiation Interview Adherence Scale for Smoking Cessation: A psychometric evaluation.

J Subst Abuse Treat 2021 Jul 20;126:108398. Epub 2021 Apr 20.

Yale University School of Medicine Department of Emergency Medicine 464 Congress Avenue, Suite 260, New Haven, CT 06519, USA; Yale School of Public Health, 60 College St., New Haven, CT 06510, USA; Yale Cancer Center, 333 Cedar St., New Haven, CT 06510, USA.

Practitioner adherence to the Brief Negotiation Interview (BNI) for high-risk alcohol consumption and opioid use disorder can be measured using the BNI Adherence Scale, for alcohol (BAS-A) and opioids (BAS-O), respectively. However, no psychometrically validated brief intervention adherence scale for smoking cessation has been reported in the literature. Our objective was to develop and examine the psychometric properties of a BAS for smoking cessation (BASS). Read More

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NMJ-Analyser identifies subtle early changes in mouse models of neuromuscular disease.

Sci Rep 2021 Jun 10;11(1):12251. Epub 2021 Jun 10.

MRC Unit for Lifelong Health and Ageing, Department of Population Science and Experimental Medicine, University College London, London, WC1E 6BT, UK.

The neuromuscular junction (NMJ) is the peripheral synapse formed between a motor neuron axon terminal and a muscle fibre. NMJs are thought to be the primary site of peripheral pathology in many neuromuscular diseases, but innervation/denervation status is often assessed qualitatively with poor systematic criteria across studies, and separately from 3D morphological structure. Here, we describe the development of 'NMJ-Analyser', to comprehensively screen the morphology of NMJs and their corresponding innervation status automatically. Read More

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Collagen VI as a driver and disease biomarker in human fibrosis.

FEBS J 2021 Jun 9. Epub 2021 Jun 9.

Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Science, University of Oxford, UK.

Fibrosis of visceral organs such as the lungs, heart, kidneys and liver remains a major cause of morbidity and mortality and is also associated with many other disorders, including cancer and metabolic disease. In this review, we focus upon the microfibrillar collagen VI, which is present in the extracellular matrix (ECM) of most tissues. However, expression is elevated in numerous fibrotic conditions, such as idiopathic pulmonary disease (IPF), and chronic liver and kidney diseases. Read More

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Capturing Death in Animated Films: Can Films Stimulate Parent-Child Conversations about Death?

Cogn Dev 2021 Jul-Sep;59. Epub 2021 May 25.

University of Rochester.

We present three studies examining death in children's animated films. Study 1 is a content analysis of 49 films. We found that death is often portrayed in films, but many deaths occurred off-screen. Read More

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Novel Phenolic Compounds as Potential Dual EGFR and COX-2 Inhibitors: Design, Semisynthesis, in vitro Biological Evaluation and in silico Insights.

Drug Des Devel Ther 2021 31;15:2325-2337. Epub 2021 May 31.

Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, Aljouf, 72341, Saudi Arabia.

Introduction: Epidermal growth factor receptor (EGFR) inhibition is an imperative therapeutic approach targeting various types of cancer including colorectal, lung, breast, and pancreatic cancer types. Moreover, cyclooxygenase-2 (COX-2) is frequently overexpressed in different types of cancers and has a role in the promotion of malignancy, apoptosis inhibition, and metastasis of tumor cells. Combination therapy has been emerged to improve the therapeutic benefit against cancer and curb intrinsic and acquired resistance. Read More

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Development of a chitosan and hyaluronic acid hydrogel with potential for bioprinting utilization: A preliminary study.

J Biomater Appl 2021 Jun 9:8853282211024164. Epub 2021 Jun 9.

Center for Natural Sciences and Humanities (CCNH), Federal University of ABC, São Bernardo do Campo, Sao Paulo, Brazil.

Bioprinting is a technique that has been applied in the areas of tissue engineering and regenerative medicine (TERM). Natural polymer-based hydrogels are known for their favorable biocompatible properties, as well as attractive biomaterials for cell encapsulation. These hydrogels provide an aqueous three-dimensional environment with biologically relevant chemical and physical signals, mimicking the natural environment of the extracellular matrix (ECM). Read More

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Modeling adaptive drug resistance of colorectal cancer and therapeutic interventions with tumor spheroids.

Exp Biol Med (Maywood) 2021 Jun 8:15353702211014185. Epub 2021 Jun 8.

Department of Biomedical Engineering, The University of Akron, Akron, OH 44325, USA.

Drug resistance is a major barrier against successful treatments of cancer patients. Various intrinsic mechanisms and adaptive responses of tumor cells to cancer drugs often lead to failure of treatments and tumor relapse. Understanding mechanisms of cancer drug resistance is critical to develop effective treatments with sustained anti-tumor effects. Read More

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The Discovery, Development, and Demonstration of Three Caged Compounds.

Photochem Photobiol 2021 Jun 8. Epub 2021 Jun 8.

Department of Chemistry, Marquette University, P. O. Box 1881, Milwaukee, 53202-1881.

An overview of the history, mechanistic aspects, and applications is provided for p-hydroxyphenacyl (pHP) and benzoin photoremovable protecting groups, which release biologically important leaving groups upon photolysis with UV light. Also discussed is (7-diethylaminocoumarin-4-yl)methyl (DEACM), a photoremovable protecting group that absorbs visible light. These are followed by the α-keto amides and naphtho- and benzothiophene-2-carboxanilides as caging groups, which eliminate leaving groups via photochemically produced zwitterionic intermediates. Read More

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Titanium-coated polypropylene mesh as innovative bioactive material in conservatives mastectomies and pre-pectoral breast reconstruction.

Bioact Mater 2021 Dec 19;6(12):4640-4653. Epub 2021 May 19.

San Rossore Breast Unit, Pisa 56122, Italy.

Breast reconstruction is rapidly evolving, thanks to the growing acceptance of synthetic meshes as innovative biomaterials. 276 patients undergoing mastectomy (total of 328 mastectomies) were analyzed in a retrospective observational study to evaluate the pre-pectoral immediate breast reconstruction (IBR) using an implant wrapped with Titanium-Coated Polypropylene Mesh (TCPM) vs. patients treated with tissue expander (TE), equally placed pre-pectorally (and wrapped with the same TCPM in 74. Read More

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December 2021

The HD-Domain Metalloprotein Superfamily: An Apparent Common Protein Scaffold with Diverse Chemistries.

Catalysts 2020 Oct 15;10(10). Epub 2020 Oct 15.

Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02453, USA.

The histidine-aspartate (HD)-domain protein superfamily contains metalloproteins that share common structural features but catalyze vastly different reactions ranging from oxygenation to hydrolysis. This chemical diversion is afforded by (i) their ability to coordinate most biologically relevant transition metals in mono-, di-, and trinuclear configurations, (ii) sequence insertions or the addition of supernumerary ligands to their active sites, (iii) auxiliary substrate specificity residues vicinal to the catalytic site, (iv) additional protein domains that allosterically regulate their activities or have catalytic and sensory roles, and (v) their ability to work with protein partners. More than 500 structures of HD-domain proteins are available to date that lay out unique structural features which may be indicative of function. Read More

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October 2020

Chemical synthesis and biological activity of peptides incorporating an ether bridge as a surrogate for a disulfide bond.

Chem Sci 2020 Jul 8;11(30):7927-7932. Epub 2020 Jul 8.

School of Food and Biological Engineering, Hefei University of Technology Hefei Anhui 230009 China

Disulfide bridges contribute to the definition and rigidity of polypeptides, but they are inherently unstable in reducing environments and in the presence of isomerases and nucleophiles. Strategies to address these deficiencies, ideally without significantly perturbing the structure of the polypeptide, would be of great interest. One possible surrogate for the disulfide bridge is a simple thioether, but these are susceptible to oxidation. Read More

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A novel bioengineered fragment peptide of Vasostatin-1 exerts smooth muscle pharmacological activities and anti-angiogenic effects via blocking VEGFR signalling pathway.

Comput Struct Biotechnol J 2021 3;19:2664-2675. Epub 2021 May 3.

Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Taipa, Macau SAR, China.

Chromogranin A (CgA) is a hydrophilic glycoprotein released by post-ganglionic sympathetic neurons. CgA consists of a single peptide chain containing numerous paired basic residues, which are typical cleavage sites in prohormones to generate bioactive peptides. It is recognized as a diagnostic and prognostic serum marker for neuroendocrine tumours. Read More

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Activity of trastuzumab emtansine (T-DM1) in 3D cell culture.

Breast Cancer Res Treat 2021 Jun 5. Epub 2021 Jun 5.

Roche Tissue Diagnostics, 1910 E Innovation Park Drive, Tucson, AZ, 85755, USA.

Background: Cell spheroids and aggregates generated from three-dimensional (3D) cell culture methods are similar to in vivo tumors in terms of tissue morphology, biology, and gene expression, unlike cells grown in 2D cell cultures. Breast cancer heterogeneity is one of the main drug resistant mechanisms and needs to be overcome in order to increase the efficacy of drug activity in its treatments.

Methods: We performed a unique 3D cell culture and drug efficacy study with trastuzumab emtansine (Kadcyla®, T-DM1) across five breast cancer cell lines (BT-474, SK-BR-3, MDA-MB-361, MDA-MB-175, and MCF-7) that were previously investigated in 2D cell culture. Read More

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The concept of protein folding/unfolding and its impacts on human health.

Adv Protein Chem Struct Biol 2021 22;126:227-278. Epub 2021 Feb 22.

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran. Electronic address:

Proteins have evolved in specific 3D structures and play different functions in cells and determine various reactions and pathways. The newly synthesized amino acid chains once depart ribosome must crumple into three-dimensional structures so can be biologically active. This process of protein that makes a functional molecule is called protein folding. Read More

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February 2021

Identification of three novel pathogenic mutations in sarcomere genes associated with familial hypertrophic cardiomyopathy based on multi-omics study.

Clin Chim Acta 2021 Jun 1;520:43-52. Epub 2021 Jun 1.

Department of Cardiovascular Ultrasound, The First Affiliated Hospital of China Medical University, Shenyang 110001, China. Electronic address:

Background: Familial hypertrophic cardiomyopathy (HCM) is a leading cause of sudden cardiac death, but exhibits heterogeneous clinical features. A major research focus is to identify specific ultrasonic phenotypes, and causal gene mutations, as well as to elucidate the possible metabolic pathogenic effects in familial HCM through multi-omics study.

Methods: Nine members of two familial HCM pedigrees were enrolled in this study. Read More

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Engineering a Blue Light Inducible SpyTag System (BLISS).

J Am Chem Soc 2021 Jun 2. Epub 2021 Jun 2.

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware 19716, United States.

The SpyCatcher/SpyTag protein conjugation system has recently exploded in popularity due to its fast kinetics and high yield under biologically favorable conditions in both and intracellular settings. The utility of this system could be expanded by introducing the ability to spatially and temporally control the conjugation event. Taking inspiration from photoreceptor proteins in nature, we designed a method to integrate light dependency into the protein conjugation reaction. Read More

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Rheostat functional outcomes occur when substitutions are introduced at nonconserved positions that diverge with speciation.

Protein Sci 2021 Jun 2. Epub 2021 Jun 2.

Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, Kansas, USA.

When amino acids vary during evolution, the outcome can be functionally neutral or biologically-important. We previously found that substituting a subset of nonconserved positions, "rheostat" positions, can have surprising effects on protein function. Since changes at rheostat positions can facilitate functional evolution or cause disease, more examples are needed to understand their unique biophysical characteristics. Read More

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In situ determination of secretory kinase Fam20C from living cells using fluorescence correlation spectroscopy.

Talanta 2021 Sep 29;232:122473. Epub 2021 Apr 29.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. Electronic address:

Secretory proteins constitute a biologically crucial subset of proteins for regulation of some pathological and physiological processes, and they have become very important biomarkers in clinical diagnosis and therapeutic targets. So far, secretory protein functions and mechanisms have not been fully understood due to methodological limitations in detection of low-abundance proteins against medium background. Here, we propose a strategy to determine secretory protein from living cells in situ using fluorescence correlation spectroscopy (FCS). Read More

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September 2021

The Potential Mechanisms of High-Velocity, Low-Amplitude, Controlled Vertebral Thrusts on Neuroimmune Function: A Narrative Review.

Medicina (Kaunas) 2021 May 27;57(6). Epub 2021 May 27.

Centre for Chiropractic Research, New Zealand College of Chiropractic, Auckland 1060, New Zealand.

The current COVID-19 pandemic has necessitated the need to find healthcare solutions that boost or support immunity. There is some evidence that high-velocity, low-amplitude (HVLA) controlled vertebral thrusts have the potential to modulate immune mediators. However, the mechanisms of the link between HVLA controlled vertebral thrusts and neuroimmune function and the associated potential clinical implications are less clear. Read More

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Comprehensive Evaluation of Late Season Peach Varieties ( L.): Fruit Nutritional Quality and Phytochemicals.

Molecules 2021 May 10;26(9). Epub 2021 May 10.

Fruit Growing Institute, 4000 Plovdiv, Bulgaria.

Peaches are one of the most preferred seasonal fruits, and a reliable source of nutrients. They possess biologically active substances that largely differ among varieties. Hence, revealing the potential of several late season peaches is of present interest. Read More

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Synthesis, Characterization, and In Vitro Insulin-Mimetic Activity Evaluation of Valine Schiff Base Coordination Compounds of Oxidovanadium(V).

Biomedicines 2021 May 17;9(5). Epub 2021 May 17.

Department of Inorganic Chemistry, Faculty of Chemistry, University of Bucharest, 23 Dumbrava Roşie, 020464-Bucharest, Romania.

Type 2 diabetes became an alarming global health issue since the existing drugs do not prevent its progression. Herein, we aimed to synthesize and characterize a family of oxidovanadium(V) complexes with Schiff base ligands derived from L-/D-valine (val) and salicylaldehyde (sal) or -vanillin (van) as insulin-mimetic agents and to assess their potential anti-diabetic properties. Two new oxidovanadium(V) complexes, [{VO(R-salval)(HO)}(μ-O){VO(R-salval)}] and [{VO(R-vanval)(CHOH)}(μ-O)], and their S-enantiomers were synthesized and characterized. Read More

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Synthesis of Isothiocyanates Using DMT/NMM/TsO as a New Desulfurization Reagent.

Molecules 2021 May 6;26(9). Epub 2021 May 6.

Faculty of Chemistry, Institute of Organic Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

Thirty-three alkyl and aryl isothiocyanates, as well as isothiocyanate derivatives from esters of coded amino acids and from esters of unnatural amino acids (6-aminocaproic, 4-(aminomethyl)benzoic, and tranexamic acids), were synthesized with satisfactory or very good yields (25-97%). Synthesis was performed in a "one-pot", two-step procedure, in the presence of organic base (EtN, DBU or NMM), and carbon disulfide via dithiocarbamates, with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TsO) as a desulfurization reagent. For the synthesis of aliphatic and aromatic isothiocyanates, reactions were carried out in a microwave reactor, and selected alkyl isothiocyanates were also synthesized in aqueous medium with high yields (72-96%). Read More

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Bioactive Components of and Their Potential Antidiabetic Properties: A Review.

Molecules 2021 May 20;26(10). Epub 2021 May 20.

Nutrition, Metabolism and Cardiovascular Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Malaysia, No.1, Jalan Setia Murni U13/52, Seksyen U13 Setia Alam, Shah Alam 40170, Malaysia.

The utilization of therapeutic plants is expanding around the globe, coupled with the tremendous expansion of alternative medicine and growing demand in health treatment. Plants are applied in pharmaceuticals to preserve and expand health-physically, mentally and as well as to treat particular health conditions and afflictions. There are more than 600 families of plants identified so far. Read More

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The Known Unknowns of the Immune Response to .

J Fungi (Basel) 2021 May 11;7(5). Epub 2021 May 11.

Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA.

Coccidioidomycosis, otherwise known as Valley Fever, is caused by the dimorphic fungi and . While most clinical cases present with self-limiting pulmonary infection, dissemination of spp. results in prolonged treatment and portends higher mortality rates. Read More

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Techniques for RNA extraction from cells cultured in starPEG-heparin hydrogels.

Open Biol 2021 Jun 2;11(6):200388. Epub 2021 Jun 2.

Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove, Australia.

Three-dimensional (3D) cell culture models that provide a biologically relevant microenvironment are imperative to investigate cell-cell and cell-matrix interactions . Semi-synthetic star-shaped poly(ethylene glycol) (starPEG)-heparin hydrogels are widely used for 3D cell culture due to their highly tuneable biochemical and biomechanical properties. Changes in gene expression levels are commonly used as a measure of cellular responses. Read More

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Structure of Biologically Active Benzoxazoles: Crystallography and DFT Studies.

Acta Chim Slov 2021 Mar;68(1):144-150

Using X-ray single crystal diffraction, the crystal structures of biologically active benzoxazole derivatives were determined. DFT calculation was performed with standard 6-31G*(d), 6-31G** and 6-31+G* basis set to analyze the molecular geometry and compare with experimentally obtained X-ray crystal data of compounds. The calculated HOMO-LUMO energy gap in compound 2 (2-(2-hydroxynaphtalen-1-yl)-4-methyl-7-isopropyl-1,3-benzoxazol-5-ol) is 3. Read More

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Alkaloids Self-Assembled Supramolecular Nanocapsules with Enhanced Antioxidant and Cytotoxic Activities.

ACS Omega 2021 May 27;6(18):11954-11963. Epub 2021 Apr 27.

Department of Chemistry, School of Sciences & Engineering, The American University in Cairo, AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.

Amphiphilic macrocycles, such as -sulfonatocalix[6]arenes (-SC6), have demonstrated great potential in designing synthetic nanovesicles based on self-assembly approaches. These supramolecular nanovesicles are capable of improving the solubility, stability, and biological activity of various drugs. In the present study, the biologically active harmala alkaloid-rich fraction (HARF) was extracted from . Read More

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Impacts of solvation on photo-damage of polypeptides: Modulation and biological implications.

J Photochem Photobiol B 2021 Jul 20;220:112214. Epub 2021 May 20.

Department of Chemistry, College of Chemical Engineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, P R, China. Electronic address:

We investigate the photon/matter interactions between soft X-rays and three selected polypeptides, poly-glycine (poly-Gly), poly-L-arginine (poly-Arg), and poly-l-lysine (poly-Lys), where the effects of molecular packing under the influence of solvent, e.g., water, substrates (Au foil or Si wafer) and X-ray irradiation under different durations were systematically investigated. Read More

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Social network distribution of syphilis self-testing among men who have sex with men in China: study protocol for a cluster randomized control trial.

BMC Infect Dis 2021 May 27;21(1):491. Epub 2021 May 27.

Dermatology Hospital of Southern Medical University, Guangzhou, Guangdong, China.

Background: Syphilis is a common sexually transmitted infection (STI) among men who have sex with men (MSM). Increasing syphilis testing is important to syphilis control. However, in low- and middle-income countries like China, syphilis testing rates remain low among MSM. Read More

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Implicit coordination for 3D underwater collective behaviors in a fish-inspired robot swarm.

Sci Robot 2021 Jan;6(50)

Harvard University, John A. Paulson School of Engineering and Applied Sciences, Cambridge, MA 02138, USA.

Many fish species gather by the thousands and swim in harmony with seemingly no effort. Large schools display a range of impressive collective behaviors, from simple shoaling to collective migration and from basic predator evasion to dynamic maneuvers such as bait balls and flash expansion. A wealth of experimental and theoretical work has shown that these complex three-dimensional (3D) behaviors can arise from visual observations of nearby neighbors, without explicit communication. Read More

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January 2021