Life Sci 2021 Aug 29;278:119553. Epub 2021 Apr 29.
The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), PB 1262, Chennai 600034, Tamil Nadu, India; JBM Inc., 3-1-14, Higashi Koiwa, Edogawa, Tokyo 133-0052, Japan; University of Yamanashi -Faculty of Medicine, 1110, Shimokato, Chuo, Yamanashi 409-3898, Japan; GN Corporation Co. Ltd., 3-8, Wakamatsu, Kofu, Yamanashi 400-0866, Japan. Electronic address:
Aims: We have evaluated the potential of a three-dimensional (3D) thermoreversible gelation polymer (TGP) matrix in enhancing miRNA 140 expression (a biomarker correlating with homeostasis and cartilage regeneration) during the in vitro expansion of osteoarthritis (OA)-affected human chondrocytes.
Materials And Methods: OA-chondrocytes were cultured in two-dimensional (2D) monolayer followed by culture in 3D-TGP. miRNA 140 expression levels in cell culture supernatant followed by expression in the cell lysate of both 2D and 3D-TGP cultures were analyzed. Read More