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Multi-level multi-modality (PET and CT) fusion radiomics: Prognostic modeling for non-small cell lung carcinoma.

Phys Med Biol 2021 Sep 20. Epub 2021 Sep 20.

Division of Nuclear Medicine, University Hospital of Geneva, 24 Rue Micheli-du-Crest, 1211 Geneva 4, Geneva, SWITZERLAND.

We developed multi-modality radiomic models by integrating information extracted from 18F-FDG PET and CT images using feature- and image-level fusions, toward improved prognosis for non-small cell lung carcinoma (NSCLC) patients. Two independent cohorts of NSCLC patients from two institutions (87 and 95 patients) were cycled as training and testing datasets. Fusion approaches were applied at two levels, namely feature- and image-levels. Read More

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September 2021

Evaluation of a Digital Handheld Hydrogen Breath Monitor to Diagnose Lactose Malabsorption: An Interventional Crossover Study.

JMIR Form Res 2021 Sep 20. Epub 2021 Sep 20.

Duke Early Phase Clinical Research Unit, Durham, US.

Background: Lactose malabsorption is a common condition that affects a broad segment of the population. Clinical diagnosis based on symptom recall can be unreliable and conventional testing can be inconvenient, requiring expensive lab-based equipment conducted in a clinical setting.

Objective: To assess the performance of a digital handheld hydrogen breath monitor (GIMate) in diagnosing lactose malabsorption compared to an FDA cleared device (H2 Check) for the same indication. Read More

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September 2021

Reliability of performing ultrasound derived SWE and fat fraction in adult livers.

Clin Imaging 2021 Sep 17;80:424-429. Epub 2021 Sep 17.

Rocky Vista University, Ivins, UT, USA.

Purpose: The aim of the study was to test the reproducibility of performing conventional point shear wave elastography (pSWE), auto-pSWE, and ultrasound derived fat fraction (UDFF) in adult livers.

Methods: The Institutional Review Board approved the study and all participants provided written informed consent. Conventional pSWE (obtaining 10 measurements through 10 acquisitions), auto-pSWE (automatically obtaining 15 measurements by a single acquisition), and UDFF (one measurement obtained by one acquisition) of the liver were prospectively performed in 21 participants (10 men, 11 women, mean age 63y) by senior and junior operators in February-May 2021. Read More

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September 2021

RMplex: An efficient method for analyzing 30 Y-STRs with high mutation rates.

Forensic Sci Int Genet 2021 Sep 11;55:102595. Epub 2021 Sep 11.

Department of Genetic Identification, Erasmus MC, University Medical Center Rotterdam, Rotterdam, the Netherlands. Electronic address:

Y-chromosomal short tandem repeats (Y-STRs) with high mutation rates are recognized as valuable genetic markers for differentiating paternally related men, who typically cannot be separated with standard Y-STRs, and were shown to provide paternal lineage differentiation on a higher resolution level than standard Y-STRs. Both features make Y-STRs with high mutation rates relevant in criminal casework, particularly in sexual assault cases involving highly unbalanced male-female DNA mixtures that often fail autosomal forensic STR profiling for the male donor. Previously, the number of known Y-STRs with mutation rates higher than 10 per locus per generation termed rapidly mutating Y-STRs (RM Y-STRs) was limited to 13, which has recently been overcome by the discovery and characterization of 12 additional RM Y-STRs. Read More

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September 2021

Cross-cultural adaptation and psychometric validation of the Indonesian version of the Oxford Hip Score.

Bone Jt Open 2021 Sep;2(9):765-772

Orthopaedic and Traumatology, Fatmawati General Hospital, Jakarta, Indonesia.

Aims: The aim of this study was to perform a cross-cultural adaptation of Oxford Hip Score (OHS) to Indonesian, and to evaluate its psychometric properties.

Methods: We performed a cross-cultural adaptation of Oxford Hip Score into Indonesian language (OHS-ID) and determined its internal consistency, test-retest reliability, measurement error, floor-ceiling effect, responsiveness, and construct validity by hypotheses testing of its correlation with Harris Hip Score (HHS), vsual analogue scale (VAS), and Short Form-36 (SF-36). Adults (> 17 years old) with chronic hip pain (osteoarthritis or osteonecrosis) were included. Read More

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September 2021

How physicians evaluate patients with dementia who present with shortness of breath.

J Am Geriatr Soc 2021 Sep 20. Epub 2021 Sep 20.

Center for the Study of Healthcare Innovation, Implementation, and Policy, VA Greater Los Angeles Healthcare System, Los Angeles, California, USA.

Background: Whether the presence of dementia in patients makes it difficult for physicians to assess the risk such patients might have for serious conditions such as pulmonary embolism (PE) is unknown. Our objective was to examine the differential association of four clinical factors (deep venous thrombosis (DVT)/PE, malignancy, recent surgery, and tachycardia) with PE testing for patients with dementia compared to patients without dementia.

Methods: We performed a cross-sectional study of emergency department (ED) visits to 104 Veterans Affairs (VA) hospitals from 2011 to 2018 by patients aged 60 years and over presenting with shortness of breath (SOB). Read More

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September 2021

Reference and point-of-care testing for G6PD deficiency: Blood disorder interference, contrived specimens, and fingerstick equivalence and precision.

PLoS One 2021 20;16(9):e0257560. Epub 2021 Sep 20.

PATH, Seattle, Washington, United States of America.

Certain clinical indications and treatments such as the use of rasburicase in cancer therapy and 8-aminoquinolines for Plasmodium vivax malaria treatment would benefit from a point-of-care test for glucose-6-phosphate dehydrogenase (G6PD) deficiency. Three studies were conducted to evaluate the performance of one such test: the STANDARD™ G6PD Test (SD BIOSENSOR, South Korea). First, biological interference on the test performance was evaluated in specimens with common blood disorders, including high white blood cell (WBC) counts. Read More

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September 2021

Immunological assessment of SARS-CoV-2 infection in pregnancy from diagnosis to delivery: A multicentre prospective study.

PLoS One 2021 20;16(9):e0253090. Epub 2021 Sep 20.

UCD School of Medicine, National Maternity Hospital, Dublin, Ireland.

Background: Background Population-based data on SARS-CoV-2 infection in pregnancy and assessment of passive immunity to the neonate, is lacking. We profiled the maternal and fetal response using a combination of viral RNA from naso-pharyngeal swabs and serological assessment of antibodies against SARS-CoV-2.

Methods: This multicentre prospective observational study was conducted between March 24th and August 31st 2020. Read More

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September 2021

Carbapenem and Colistin Resistant Klebsiella Pneumoniae ST14 and ST2096 Dominated in Two Hospitals in Turkey.

Clin Lab 2021 Sep;67(9)

Background: The determination of clonal interactions between microorganisms is very important in epidemiological studies. In the present study, we aimed to evaluate the resistance mechanisms and genetic relationships of carbapenem and colistin resistant Klebsiella pneumoniae (K. pneumoniae) strains isolated from inpatients at two university hospitals in Turkey. Read More

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September 2021

High incidences of chromosomal aberrations and Y chromosome micro-deletions as prominent causes for recurrent pregnancy losses in highly ethnic and consanguineous population.

Arch Gynecol Obstet 2021 Sep 20. Epub 2021 Sep 20.

School of Life and Basic Sciences, Jaipur National University, Jaipur, 302025, India.

Purpose: Recurrent Miscarriages (RM) commonly complicates the reproductive outcome where prominently chromosomal aberrations and molecular factors lead to recurrent miscarriages. We investigated couples with RM for cytogenetic abnormalities and Y chromosome microdeletions in males along with detection of aneuploidies de novo in the product of conception from a highly ethnic consanguineous population (Kashmir, North India) .

Study Design: Chromosomal analysis was done by Karyotyping on peripheral blood lymphocyte cultures and analyzed by Cytovision software Version 3. Read More

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September 2021

Gaps in Public Awareness About BRCA and Genetic Testing in Prostate Cancer: Social Media Landscape Analysis.

JMIR Cancer 2021 Sep 20;7(3):e27063. Epub 2021 Sep 20.

Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA, United States.

Background: Genetic testing, particularly for BRCA1/2, is increasingly important in prostate cancer (PCa) care, with impact on PCa management and hereditary cancer risk. However, the extent of public awareness and online discourse on social media is unknown, and presents opportunities to identify gaps and enhance population awareness and uptake of advances in PCa precision medicine.

Objective: The objective of this study was to characterize activity and engagement across multiple social media platforms (Twitter, Facebook, and YouTube) regarding BRCA and genetic testing for PCa compared with breast cancer, which has a long history of public awareness, advocacy, and prominent social media presence. Read More

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September 2021

Clinical decision making is improved by BioFire Pneumonia Plus in suspected lower respiratory tract infection after lung transplantation - Results of the prospective DBATE-IT Study.

Transpl Infect Dis 2021 Sep 20. Epub 2021 Sep 20.

Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany.

Background: Lower respiratory tract infections (LRTI) are a significant cause of morbidity and mortality in lung transplant (LTx) recipients. Timely and precise pathogen detection is vital to successful treatment. Multiplex PCR kits with short turnover times like the BioFire PneumoniaPlus (BFPPp, manufactured by bioMérieux), may be a valuable addition to conventional tests. Read More

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September 2021

A review of the toxicology of oil in vertebrates: what we have learned following the oil spill.

J Toxicol Environ Health B Crit Rev 2021 Sep 19:1-40. Epub 2021 Sep 19.

Sea Mammal Research Unit, Scottish Oceans Institute, University of St Andrews, St Andrews, UK.

In the wake of the (DWH) oil spill, a number of government agencies, academic institutions, consultants, and nonprofit organizations conducted lab- and field-based research to understand the toxic effects of the oil. Lab testing was performed with a variety of fish, birds, turtles, and vertebrate cell lines (as well as invertebrates); field biologists conducted observations on fish, birds, turtles, and marine mammals; and epidemiologists carried out observational studies in humans. Eight years after the spill, scientists and resource managers held a workshop to summarize the similarities and differences in the effects of DWH oil on vertebrate taxa and to identify remaining gaps in our understanding of oil toxicity in wildlife and humans, building upon the cross-taxonomic synthesis initiated during the Natural Resource Damage Assessment. Read More

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September 2021

Resource implications following expansion of computed tomography coronary angiography: An Australian experience.

J Med Imaging Radiat Oncol 2021 Sep 19. Epub 2021 Sep 19.

Peninsula Health, Melbourne, Victoria, Australia.

Introduction: To determine the downstream utilisation of Computed Tomography Coronary Angiography (CTCA) in a single Australian tertiary centre.

Methods: A single-centre retrospective study analysed 1460 patients undergoing CTCA between 1st January 2015 to 31st December 2018 at a tertiary hospital in Victoria, Australia, with a catchment area of 500,000 people. The coronary stenosis grading, plaque characteristics and coronary calcium score were identified. Read More

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September 2021

A methylation study implicates the rewiring of brain neural circuits during puberty in the emergence of sex differences in depression symptoms.

J Child Psychol Psychiatry 2021 Sep 19. Epub 2021 Sep 19.

Center for Biomarker Research and Precision Medicine, Virginia Commonwealth University, Richmond, VA, USA.

Background: Women are 1.5-3 times more likely to suffer from depression than men. This sex bias first emerges during puberty and then persists across the reproductive years. Read More

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September 2021

Public Attitudes and Factors of COVID-19 Testing Hesitancy in the United Kingdom and China: Comparative Infodemiology Study.

JMIR Infodemiology 2021 Jan-Dec;1(1):e26895. Epub 2021 Aug 27.

School of Public Health Fudan University Shanghai China.

Background: Massive community-wide testing has become the cornerstone of management strategies for the COVID-19 pandemic.

Objective: This study was a comparative analysis between the United Kingdom and China, which aimed to assess public attitudes and uptake regarding COVID-19 testing, with a focus on factors of COVID-19 testing hesitancy, including effectiveness, access, risk perception, and communication.

Methods: We collected and manually coded 3856 UK tweets and 9299 Chinese Sina Weibo posts mentioning COVID-19 testing from June 1 to July 15, 2020. Read More

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Cost-effective screening using a two-antibody panel for detecting mismatch repair deficiency in sporadic colorectal cancer.

World J Clin Cases 2021 Aug;9(24):6999-7008

Division of Colon and Rectal Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, South Korea.

Background: The microsatellite instability (MSI) test and immunohistochemistry (IHC) are widely used to screen DNA mismatch repair (MMR) deficiency in sporadic colorectal cancer (CRC). For IHC, a two-antibody panel of MLH1 and MSH2 or four-antibody panel of MLH1, MSH2, PMS2, and MSH6 are used. In general, MSI is known as a more accurate screening test than IHC. Read More

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Mechanical Stimulation Muscle Activity Is Necessary for the Maturation of Tendon Multiscale Mechanics During Embryonic Development.

Front Cell Dev Biol 2021 3;9:725563. Epub 2021 Sep 3.

Department of Biomedical Engineering, Pennsylvania State University, University Park, PA, United States.

During embryonic development, tendons transform into a hypocellular tissue with robust tensile load-bearing capabilities. Previous work suggests that this mechanical transformation is due to increases in collagen fibril length and is dependent on mechanical stimulation muscle activity. However, the relationship between changes in the microscale tissue structure and changes in macroscale tendon mechanics is still unclear. Read More

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September 2021

An Outbreak of Carbapenem-Resistant of K57 Capsular Serotype in an Emergency Intensive Care Unit of a Teaching Hospital in China.

Front Public Health 2021 1;9:724212. Epub 2021 Sep 1.

Clinical Laboratory of Taian City Central Hospital, Tai'an, China.

The emergence of carbapenem-resistant hypervirulent (CR-hvKP) strains has increased the threat posed by . Here, we described an outbreak of 32 CR-hvKP isolates from the emergency intensive care unit (EICU) of a teaching hospital in China. Thirty-two CRKp isolates were collected from six patients and their surrounding environment in EICU. Read More

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September 2021

Extending the Applicability of and Chicken Chorioallantoic Membrane Assays to Study Cytostatic Activity in Neuroblastoma Cells.

Front Oncol 2021 1;11:707366. Epub 2021 Sep 1.

Research Group for Molecular Biology and Nanomedicine, Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czechia.

Purpose: The chick chorioallantoic membrane (CAM) assay can provide an alternative versatile, cost-effective, and ethically less controversial model for reliable screening of drugs. In the presented work, we demonstrate that CAM assay ( and ) can be simply employed to delineate the effects of cisplatin (CDDP) and ellipticine (Elli) on neuroblastoma (Nbl) cells in terms of their growth and metastatic potential.

Methods: The Nbl UKF-NB-4 cell line was established from recurrent bone marrow metastases of high-risk Nbl (stage IV, amplification, 7q21 gain). Read More

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September 2021

Adapted full-face snorkel masks as an alternative for COVID-19 personal protection during aerosol generating procedures in South Africa: A multi-centre, non-blinded simulation study.

Afr J Emerg Med 2021 Sep 13. Epub 2021 Sep 13.

Department of Anaesthesia and Perioperative Medicine, Faculty of Health Sciences, University of Cape Town, and Groote Schuur Hospital, Cape Town, South Africa.

Introduction: SARS-CoV-2 has resulted in increased worldwide demand for personal protective equipment (PPE). With pressure from ongoing epidemic and endemic episodes, we assessed an adapted snorkel mask that provides full-face protection for healthcare workers (HCWs), particularly during aerosol-generating procedures. These masks have a custom-made adaptor which allows the fitment of standard medical respiratory filters. Read More

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September 2021

Evaluation of the ESGE recommendations for COVID-19 pre-endoscopy risk-stratification in a high-volume center in Germany.

Endosc Int Open 2021 Oct 16;9(10):E1556-E1560. Epub 2021 Sep 16.

Department of Gastroenterology and Infectious Diseases, University Hospital of Augsburg, Augsburg, Germany.

The European Society of Gastrointestinal Endoscopy (ESGE) has defined COVID-19 infection prevention and control strategies within the endoscopy unit. These include pre-endoscopic questionnaire-based risk-stratification as well as pre-procedure viral testing. Real-life data on the effectiveness of these measures are presented here. Read More

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October 2021

Cervical Cancer Screening in South Florida Veteran Population, 2014 to 2020: Cytology and High-Risk Human Papillomavirus Correlation and Epidemiology.

Cureus 2021 Aug 17;13(8):e17247. Epub 2021 Aug 17.

Pathology and Laboratory Medicine, James A. Haley Veterans' Hospital, Tampa, USA.

Objective This project aims to use our robust women's health patient data to analyze the correlation between cytology and high-risk human papillomavirus (Hr-HPV) testing, study the performance of Hr-HPV testing for detecting cytology lesions, and examine epidemiologic measures of human papillomavirus (HPV) infections in the women's veteran population. Methods We collected patient data from 2014 to 2020 from our computerized patient record system. We performed HPV assays using the cobas® 4800 system (Roche Diagnostics, Basel, Switzerland). Read More

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Olmesartan-Induced Enteropathy: A Report of an Unusual Cause of Chronic Diarrhea.

Cureus 2021 Aug 8;13(8):e17004. Epub 2021 Aug 8.

Gastroenterology Department, General University Hospital of Patras, Patras, GRC.

Olmesartan, an angiotensin II receptor blocker indicated in the treatment of hypertension, has been associ-ated with a seronegative sprue-like enteropathy that should be considered in the differential diagnosis of patients with unexplained chronic diarrhoea. It typically presents with severe chronic diarrhoea, considerable weight loss, and villous atrophy on biopsy and may be difficult to recognize because of its clinical and histological similarities to other clinical entities. Practically, discontinuation of the drug leads to dramatic recovery of the symptoms. Read More

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Prevalence of multidrug-resistance and and genes among gram-negative clinical isolates in tertiary care hospital, Kathmandu, Nepal.

Iran J Microbiol 2021 Jun;13(3):303-311

Depatment of Microbiology, Shi-Gan International College of Sciences and Technology, Kathmandu, Nepal.

Background And Objectives: Carbapenems have been the choice of antibiotics for the treatment of infections caused by multidrug-resistant bacteria. The main objective of this study was to determine the prevalence of carbapenemase ( and ) producing isolates among and

Materials And Methods: A total of 1,151 clinical samples were collected from the patients visiting Annapurna Neurological Institute and Allied Science and Annapurna Research Centre, Kathmandu, between June 2017 and January 2018. Antibiotic susceptibility testing (AST) was performed on the and isolates using the Kirby-Bauer disk diffusion method. Read More

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The gene expression patterns of neuronal cells reveal the pathogenesis of autism.

Am J Transl Res 2021 15;13(8):8977-8987. Epub 2021 Aug 15.

Department of Neurology Medicine, The Second Hospital, Cheeloo College of Medicine, Shandong University Jinan 250033, Shandong, China.

Objective: To explore the effects of early comprehensive and specific behavioral interventions on improving social communication and attenuating anxiety in autism patients.

Methods: This study was based on the modular comprehensive analysis method and explored the neurotransmitter conduction mechanism in the pathogenesis of autism. We downloaded autism-related data from the Gene Expression Omnibus (GEO) database and performed a differential analysis, a co-expression Network Analysis (WGCNA) analysis, a GO function, and a KEGG pathway enrichment analysis. Read More

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Recall Rate in Congenital Hypothyroidism Screening: Influence of the Day of Sample Collection and Lower Cutoff.

Acta Endocrinol (Buchar) 2021 Apr-Jun;17(1):22-25

University of Medicine and Pharmacy - Clinical Biochemistry and Immunology.

Introduction: The recall rate in congenital hypothyroidism (CH) newborn screening programs depends on several factors such as primary screening strategy, sample collection guidelines, and cut-off. A recall rate of 0.05% is recommended but recall rates of 0. Read More

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October 2021

Real-life Cretan asthma registry focused on severe asthma: On behalf of 'The Cretan registry of the use of Biologics in Severe Asthma'.

Exp Ther Med 2021 Nov 1;22(5):1239. Epub 2021 Sep 1.

Laboratory of Molecular and Cellular Pneumonology, Department of Respiratory Medicine, School of Medicine, University Hospital of Heraklion, University of Crete, 71003 Heraklion, Greece.

Asthma diagnosis and management remains a challenging task for the medical community. The aim of the present study was to present the functional and inflammatory profiles of patients with difficult-to-treat asthma in a real-life clinical setting referred to the specialized asthma clinic at the University Hospital of Heraklion. The registry included a cohort of 267 patients who were referred to the severe asthma clinic. Read More

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November 2021

Monitoring Specific IgM and IgG Production Among Severe COVID-19 Patients Using Qualitative and Quantitative Immunodiagnostic Assays: A Retrospective Cohort Study.

Front Immunol 2021 3;12:705441. Epub 2021 Sep 3.

Department of Pediatrics, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.

The purpose of this study is to monitor specific anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) IgG and IgM antibody production in patients with severe forms of coronavirus disease 2019 (COVID-19) using various commercially available quantitative and qualitative tests. The sera of 23 confirmed COVID-19 patients were processed for anti-SARS-CoV-2 IgG and IgM detection. Three different immunoassays, viz. Read More

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September 2021

Development and Application of a Novel Rapid and Throughput Method for Broad-Spectrum Anti-Foodborne Norovirus Antibody Testing.

Front Microbiol 2021 3;12:670488. Epub 2021 Sep 3.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.

Foodbone norovirus (NoV) is the leading cause of acute gastroenteritis worldwide. Candidate vaccines are being developed, however, no licensed vaccines are currently available for managing NoV infections. Screening for stimulated antibodies with broad-spectrum binding activities can be performed for the development of NoV polyvalent vaccines. Read More

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September 2021