121,648 results match your criteria testes

Variant , Defective piRNA Processing, and Azoospermia.

N Engl J Med 2021 Aug 4. Epub 2021 Aug 4.

From the Division of Genetics, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton (L.N., D.F.C.); the Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland (D.F.C.); the Department of Growth and Reproduction (N.M., J.E.N., R.S., I.G., S.B.W., N.E.S., E.R.-D.M., N.J., K.A.) and the International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (N.M., J.E.N., R.S., I.G., S.B.W., N.E.S., E.R.-D.M., N.J., K.A.), Rigshospitalet, and the Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences (K.A.), University of Copenhagen, Copenhagen; the Laboratory of Molecular Neurooncology, Neuroscience Institute (R.S.), and the Institute of Biology Systems and Genetic Research (I.G.), Lithuanian University of Health Sciences, Kaunas, Lithuania; the Department of Human Genetics, Donders Institute for Brain, Cognition, and Behavior (M.S.O., G.W.H.), and the Department of Obstetrics and Gynecology (G.W.H.), Radboud University Medical Center, Nijmegen, the Netherlands; Serviço de Genética, Departamento de Patologia, Faculdade de Medicina da Universidade do Porto (F.C., C.J.M.), Instituto de Investigação e Inovação em Saúde, Universidade do Porto (F.C., C.J.M., A.M.L.), and the Institute of Molecular Pathology and Immunology of the University of Porto (A.M.L.) - all in Porto, Portugal; the Andrology and In Vitro Fertilization Laboratory, Department of Surgery (Urology), University of Utah School of Medicine, Salt Lake City (K.I.A.); the Departments of Pathology and Laboratory Medicine (F.K.) and Urology (P.N.S.), Weill Cornell Medicine, New York; and the Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom (J.A.V.).

Background: P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are short (21 to 35 nucleotides in length) and noncoding and are found almost exclusively in germ cells, where they regulate aberrant expression of transposable elements and postmeiotic gene expression. Critical to the processing of piRNAs is the protein poly(A)-specific RNase-like domain containing 1 (PNLDC1), which trims their 3' ends and, when disrupted in mice, causes azoospermia and male infertility.

Methods: We performed exome sequencing on DNA samples from 924 men who had received a diagnosis of nonobstructive azoospermia. Read More

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A Two-Generation Reproductive Toxicity Study of Lanthanum Nitrate in SD Rats.

Biol Trace Elem Res 2021 Aug 4. Epub 2021 Aug 4.

Department of Health Toxicology, Faculty of Naval Medicine, The Second Military Medical University, Shanghai, 200433, China.

In order to evaluate the effects of lanthanum nitrate on the development of the parent, offspring, and the third generation of Sprague-Dawley (SD) rats, a two-generation reproductive toxicity experiment, was conducted. Two hundred and forty specific pathogen-free (SPF) healthy SD rats were randomly divided into the control group, low-, medium-, and high-dose group, with 30 male and 30 female rats in each group. The rats in each group were given 0 mg/kg, 10. Read More

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Gene expression microarray analysis of adult testicular germ cell tumor: a comparison between pure-type seminomas and seminoma components in mixed tumors.

Virchows Arch 2021 Aug 4. Epub 2021 Aug 4.

Department of Basic Pathology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan.

We previously demonstrated a genetic evidence of the progression from seminoma to embryonal carcinoma in mixed testicular germ cell tumors (TGCTs). This process, the "reprogramming" of seminoma cells, is crucial for pathological tumorigenesis and should be kept in mind while designing clinical therapeutic strategies. We hypothesized that a comparison between pure-type seminomas and seminoma components in mixed tumors (mixed-type seminomas) could reveal early changes in the reprogramming process. Read More

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Toxicokinetic studies of di-isobutyl phthalate focusing on the exploration of gender differences in rats.

Chemosphere 2021 Jul 28;286(Pt 1):131706. Epub 2021 Jul 28.

College of Pharmacy, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea. Electronic address:

Due to the use of di-isobutyl-phthalate (DiBP) in the production of soft-polyvinyl chloride articles, it is currently a hazardous substance prevalent in human daily life. However, reports on DiBP's toxicokinetics are still very scarce. And no studies have been reported on gender differences in DiBP toxicokinetics. Read More

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Phytochemical analysis and protective effects of (cranberry) in rats () following ethylene oxide-induced oxidative insult.

Bioengineered 2021 Dec;12(1):4593-4604

Forensic Medicine Directorate (FMD), Ministry of Public Health, Kabul, Afghanistan.

The genus comprises more than 126 genera of perennial flowering plants that are commonly adapted to poor and acidic soils or epiphytic environments. Their molecular and genomic characterization is a result of the recent advent in next-generation sequencing technology. In the current research, extracts were prepared in different media, such as petroleum ether, methanol and ethanol. Read More

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December 2021

Hedgehog signal disruption, gonadal dysgenesis and reproductive disorders: Is there a link to endocrine disrupting chemicals?

Curr Res Toxicol 2020 Jun 24;1:116-123. Epub 2020 Oct 24.

Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.

Developmental exposure to chemicals that can disrupt sex hormone signaling may cause a broad spectrum of reproductive disorders. This is because reproductive development is tightly regulated by steroid sex hormones. Consequently, non-animal screening methods currently used to test chemicals for potential endocrine disrupting activities typically include steroidogenesis and nuclear receptor assays. Read More

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Sub-chronic dermal exposure to aircraft engine oils impacts the reproductive organ weights and alters hematological profiles of Sprague Dawley rats.

Curr Res Toxicol 2020 Jun 10;1:12-24. Epub 2020 Mar 10.

Air Force Research Laboratory, 711 Human Performance Wing, Airman Systems Directorate (AFRL/711 HPW/RHBB), Wright-Patterson AFB, OH 45433, USA.

There is little data available for the toxicity of used aircraft engine oils relative to their unused (new) versions. This study was conducted to determine if grade 3 (G3) and 4 (G4) aircraft engine oils in their new states (G3-N and G4-N) and their used versions (G3-U and G4-U) have the potential to induce toxicity via dermal application. Male and female Sprague Dawley rats were dermally exposed to water (control), new and used versions of G3 and G4 oils to determine the oil sub-chronic toxicity potentials. Read More

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Intrauterine exposure to diethylhexyl phthalate disrupts gap junctions in the fetal rat testis.

Curr Res Toxicol 2020 Jun 10;1:5-11. Epub 2020 Mar 10.

Division of Diet, Disease Prevention and Toxicology, National Food Institute, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.

Fetal exposure to certain phthalate esters can disrupt testis development in rodents and lead to male reproductive disorders, but with a causal link less certain in humans. Di(2-ethylhexyl) phthalate (DEHP) is one of the most common phthalates found in the environment and in rodents it is known to induce serious testis toxicity, as well as male reproductive disorders including cryptorchidism, hypospadias, impaired spermatogenesis and reduced fertility. In this study, we show that perinatal DEHP exposure disrupts gap junction localization in fetal and postnatal rat testis and correlate these findings to morphological changes. Read More

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Prevalence, management and outcome of cryptorchidism associated with gastroschisis: A systematic review and meta-analysis.

J Pediatr Surg 2021 Jul 13. Epub 2021 Jul 13.

Pediatric Surgery Unit, Department of Maternal Infantile and Urological Sciences, Sapienza University of Rome, Azienda Ospedaliero Universitaria Policlinico Umberto I, Viale Regina Elena, 324, Rome 00161, Italy. Electronic address:

Purpose: Cryptorchidism associated with gastroschisis has been poorly investigated. We aimed to assess the prevalence of this association, and to address the management and outcome of cryptorchidism in this context.

Materials And Methods: In this systematic review, we searched electronic databases (PubMed, Web of Science, and Google Scholar), without language restrictions from inception to March 31, 2021, for studies on cryptorchidism and gastroschisis co-occurrence. Read More

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Decreased angiotensin receptor 1 expression in ± AT1 Knockout mice testis results in male infertility and GnRH reduction.

Reprod Biol Endocrinol 2021 Aug 3;19(1):120. Epub 2021 Aug 3.

Institute of Nephrology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 528 Zhangheng Road, Pudong New Area, Shanghai, China.

Background: This study aimed to detect the effect of angiotensin receptor 1 (AT1) knock out (KO) on spermatogenesis and hypothalamic-pituitary-gonadal (HPG) axis hormone expression.

Methods: Normal C57BL/6 male mice were used as control group or treated with angiotensin receptor blocker, in addition heterozygous ± AT1KO mice were generated. After caged at a ratio of 2 to 1 with females, pregnancy rates of female mice were determined by detection of vaginal plugs. Read More

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Omega-3 fatty acid rescues ischaemia/perfusion-induced testicular and sperm damage via modulation of lactate transport and xanthine oxidase/uric acid signaling.

Biomed Pharmacother 2021 Jul 31;142:111975. Epub 2021 Jul 31.

Bioresources Development Centre, National Biotechnology Development Agency, Ministry of Science and Technology, Ogbomoso, Oyo State, Nigeria. Electronic address:

This study aimed to explore the potential antioxidant, anti-inflammatory, and anti-apoptotic effects of omega 3 fatty acid (Ω-3) in a rat model of testicular torsion/detorsion (T/D). Under ketamine/xylazine anaesthesia, age-matched adult male Wistar rats of comparable weight underwent sham-operation or testicular torsion by fixing the left testis rotated at 720° for two and half hours. After detorsion, animals were treated with either olive oil as vehicle or Ω-3 subcutaneously for three days. Read More

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HIV-1 establishes a sanctuary site in the testis by permeating the BTB through changes in cytoskeletal organization.

Endocrinology 2021 Aug 3. Epub 2021 Aug 3.

The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.

Studies suggest that HIV-1 invades the testis through permeation of the blood-testis barrier (BTB). The selectivity of the BTB to antiretroviral drugs makes this site a sanctuary for the virus. Little is known about how HIV-1 crosses the BTB and invades the testis. Read More

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TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos.

J Anim Sci Biotechnol 2021 Aug 3;12(1):89. Epub 2021 Aug 3.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China.

Background: Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion. To derive greater economic benefit, farmers require more female offspring (does); however, the buck-to-doe offspring sex ratio is approximately 50%. At present, artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring. Read More

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Retiform hemangioendothelioma: a rare lesion of the vulva.

J Int Med Res 2021 Aug;49(8):3000605211027783

Department of Gynecological Oncology, The First Hospital of Jilin University, Changchun, Jilin, China.

Retiform hemangioendothelioma (RH) is a rare borderline-malignant vascular tumor with specific histological characteristics, usually occurring in the limbs and trunk. We report the case of a 63-year-old woman who presented with a painless, oval nodule that had been growing slowly on her left vulva for 3 years. Magnetic resonance imaging of the pelvic cavity revealed a 4. Read More

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Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage.

Hum Exp Toxicol 2021 Aug 2:9603271211035674. Epub 2021 Aug 2.

Department of Histology and Embryology, Faculty of Medicine, 37521Trakya University, Edirne, Turkey.

The aim of the study was to investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) against methotrexate (MTX)-induced testicular damage in rats. Forty male rats were divided into equally four groups: Control group (saline solution, IP), PDTC group (100 mg/kg PDTC,IP, 10 days), MTX group (20 mg/kg MTX, IP, single dose, on the 6th day) and MTX + PDTC group (100 mg/kg PDTC, IP, 10 days and 20 mg/kg MTX, IP, single dose, on the 6th day). After 10 days, testicular tissues were excised for morphometric, histological and immunohistochemical evaluations. Read More

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Association of malignant neoplasms with semen quality in Guangdong province, China: a propensity score matching analysis.

Andrology 2021 Aug 2. Epub 2021 Aug 2.

NHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong Province, Guangzhou, Guangdong, 510080, China.

Background: Previous studies have reported lower semen quality in malignant neoplasm patients before antineoplastic treatments, but the adverse effects of malignant neoplasms on semen quality have rarely been quantitatively evaluated. In addition, due to the lack of a comparable control group and limited types of studied malignant neoplasms, the results remain inconsistent and inconclusive.

Objectives: To quantitatively evaluate the potential adverse effects of specific malignant neoplasms on semen quality. Read More

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Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2.

Endocrinology 2021 Aug 2. Epub 2021 Aug 2.

Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, P.R China.

The blood-testis barrier (BTB) and apical ectoplasmic specialization (ES), which are synchronized through the crosstalk of Sertoli cells and Sertoli-germ cells, are required for spermatogenesis and sperm release. Here, we show that Wnt5a, a noncanonical Wnt signaling pathway ligand, is predominately expressed in both the BTB and apical ES and has a specific expression pattern during the seminiferous epithelium cycle. We employed siRNA to knockdown Wnt5a expression in testis and Sertoli cells and then identified elongated spermatids that lost their polarity, and were embedded in the seminiferous epithelium. Read More

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Microsurgical Obstruction of Testes Fusion in Spodoptera litura.

J Vis Exp 2021 Jul 16(173). Epub 2021 Jul 16.

South China Normal University-Shipai Campus: South China Normal University;

Instead of using genetic methods like RNA interference (RNAi) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease Cas9, a physical barrier was microsurgically inserted between the testes of Spodoptera litura to study the impact of this microsurgery on its growth and reproduction. After inserting aluminum foil between the testes, insect molting during metamorphosis proceeded normally. Insect growth and development were not remarkably altered; however, the number of sperm bundles changed if testes fusion was stopped by the microsurgery. Read More

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The impact of antisperm antibodies on human male reproductive function: an update.

Reproduction 2021 Aug 1. Epub 2021 Aug 1.

S Amaral, University of Coimbra, University of Coimbra Center for Neuroscience and Cell Biology, Coimbra, Portugal.

Immune infertility occurs due to the presence of antisperm antibodies (ASA). This type of infertility has a relatively low prevalence (2.6-6. Read More

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Long-term Testis Cancer Survivors in Canada-Mortality Risks in a Large Population-based Cohort.

Eur Urol Open Sci 2020 Dec 20;22:54-60. Epub 2020 Nov 20.

Division of Urology, Department of Surgery, Western University, London, Ontario, Canada.

Background: Testis cancer (TC) patients are young with excellent cancer prognosis. Hence, the risk of late-onset treatment-related morbidity and mortality is of concern due to longer survival after treatment.

Objective: We set to characterize long-term survival of TC patients through a Canadian population dataset. Read More

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December 2020

Abdominoscrotal Hydrocele: An Uncommon Cause of Abdominoscrotal Cystic Swelling.

Case Rep Urol 2021 9;2021:6655127. Epub 2021 Jul 9.

Department of General Surgery, JNMC, Maharashtra, 442001, Wardha, India.

Abdominoscrotal hydrocele (ASH) consists of fluid-filled intercommunicating inguinoscrotal and abdominal sac with a characteristic hourglass-like picture on CECT, which usually affects single testis and a rare cause of abdominoscrotal cystic swelling. The precise etiology of ASH is not known. Ultrasonography is the initial diagnostic modality of choice as it demonstrates the intercommunication between the two sacs and also identifies any abnormality of the testis and genitourinary tract. Read More

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Unnecessary ultrasounds in children with undescended testes: An interrogation of the impact of the Choosing Wisely campaigns and clinical practice guidelines.

Paediatr Child Health 2021 Aug 7;26(5):299-304. Epub 2020 Oct 7.

The Division of Urology, The Hospital for Sick Children, The University of Toronto, Toronto, Ontario.

Objectives: Over the past decade, clinical practice guidelines and educational campaigns have counselled against the use of routine ultrasound (US) in the diagnosis of undescended testes (UDT). We aimed to establish whether or not there has been change in the proportion of children with UDT undergoing pre-referral US prior to referral to our centre over this period. We also sought to determine whether type of referring specialist, UDT diagnosis, and patient distance from the hospital had impacted the rate of pre-referral US. Read More

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Identification and Characterization of the Succinate Dehydrogenase Complex Iron Sulfur Subunit B Gene in the Oriental River Prawn, .

Front Genet 2021 15;12:698318. Epub 2021 Jul 15.

Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.

Previous studies have revealed that has potential functions in the male sexual differentiation and development in . through providing ATP. In this study, the functions of were further analyzed in depth using quantitative polymerase chain reaction (qPCR), hybridization, western-blot, and RNA interference (RNAi), combined with the histological observations. Read More

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Identification of the Bisphenol A (BPA) and the Two Analogues BPS and BPF in Cryptorchidism.

Front Endocrinol (Lausanne) 2021 14;12:694669. Epub 2021 Jul 14.

Department of Pediatric Surgery and Urology, Faculty of Medicine, Medical University of Bialystok, Białystok, Poland.

Objective: to explore the association of plasma concentrations of bisphenol A (BPA), bisphenol S (BPS), and bisphenol F (BPF) with unilateral cryptorchidism. In addition, to analyze selected demographic and intraoperative characteristics.

Design: Retrospective analysis to determine plasma concentrations of total BPA, BPS and BPF using gas chromatography - mass spectrometry (GC-MS) among prepubertal boys with cryptorchidism and prebupertal male control subjects. Read More

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Prevalence, intensity, and impacts of non-cutaneous lesions of lumpy skin disease among some infected cattle flocks in Nile Delta governorates, Egypt.

Comp Clin Path 2021 Jul 24:1-8. Epub 2021 Jul 24.

Department of pathology, Agriculture Research Center, Provincial Lab, Animal Health Research Institute, Mansoura, Egypt.

Lumpy skin disease (LSD) is one of the major viral diseases still causing great economic losses among breeding flocks of Egypt. This study was designed to focus light on non-cutaneous lesions (prevalence, intensity, and impacts) among necropsied LSD infected cattle. We selected some dairy and beef flocks (Frisian breed) located in 3 governorates (Sharkia, Dakahlia, and Kaloubia) in Nile delta, Egypt, in the period from January 2019 to January 2020 for our survey study. Read More

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Somatic-type Malignancies in Testicular Germ Cell Tumors: A Clinicopathologic Study of 63 Cases.

Am J Surg Pathol 2021 Aug 2. Epub 2021 Aug 2.

Departments of Pathology Genitourinary Medical Oncology Urology, The University of Texas MD Anderson Cancer Center, Houston, TX.

The development of somatic-type malignancies (SMs) in testicular germ cell tumors (GCTs) is a rare but well-recognized phenomenon. We studied the pathologic features of 63 GCTs with SMs in the testis (n=22) or metastases (n=41) and correlated these features with clinical outcomes. The patients with SMs in the testis (median age, 26 y) were younger than those with metastatic SMs (median age, 38. Read More

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Warning against second-generation antiandrogen for metastatic castration sensitive prostate cancer.

BJU Int 2021 Aug 1. Epub 2021 Aug 1.

Department of Nephro-Urologic Surgery and Andrology, Mie University Graduate School of Medicine.

Growing evidence showed that the use of next-generation androgen receptor axis-targeted agents (ARATs) and/or docetaxel improves both progression-free and overall survival in metastatic hormone-sensitive prostate cancer (mCSPC) (1). ARATs include abiraterone acetate, enzalutamide, and apalutamide. Abiraterone acetate blocks CYP17A1, which reduces testosterone synthesis from adrenal androgens or cholesterol in the testis, adrenal glands, and prostate cancer cells (2). Read More

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Protective effects of sinapic acid against cyclophosphamide-induced testicular toxicity via inhibiting oxidative stress, caspase-3 and NF-kB activity in BALB/c mice.

Andrologia 2021 Jul 31:e14196. Epub 2021 Jul 31.

Department of Anatomy, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Cyclophosphamide (CP), as a chemotherapeutic agent, with the generation of oxidative stress leads to testicular toxicity. Sinapic acid (SA), as a phenylpropanoid compound has therapeutic activities. This research was planned to evaluate the improving effects of SA versus testicular injury induced by CP. Read More

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Gonadal sex differentiation and early ovarian/testicular development in cultured Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel).

Theriogenology 2021 Jul 24;173:56-63. Epub 2021 Jul 24.

Nagasaki Field Station, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 1551-8 Taira-machi, Nagasaki, Nagasaki, 851-2213, Japan. Electronic address:

Pacific bluefin tuna (PBT), Thunnus orientalis, is one of the most important species for aquaculture in Japan. Recently, the reduction in muscle fat content associated with sexual maturation in farmed PBT has become a serious problem. To develop technologies for inducing sterility, detailed and reliable data on gonadal development in PBT are needed. Read More

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Testing sensation of gently squeezing the testes has diagnostic value in spinal cord injury men.

Spinal Cord Ser Cases 2021 Jul 30;7(1):67. Epub 2021 Jul 30.

Neurology, Internal Medicine, Sakura Medical Center, Toho University, Tokyo, Japan.

Study Design: Retrospective cohort study.

Objectives: Determine the diagnostic value of testing the sensation of squeezing the testes.

Setting: Research group run by the University of Antwerp. Read More

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