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Antimicrobial use, biosecurity, herd characteristics, and antimicrobial resistance in indicator Escherichia coli in ten Finnish pig farms.

Prev Vet Med 2021 Jun 10;193:105408. Epub 2021 Jun 10.

Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Finland.

We investigated connections between antimicrobial use (AMU), biosecurity, and the numbers of pigs and staff in ten Finnish farrow-to-finish herds. Data on AMU in each herd were collected for 12 months. AMU was quantified as treatment incidences per 1000 days at risk (TI) using the consensus defined daily dose calculation. Read More

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Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research.

Stem Cell Res 2021 Jun 8;54:102409. Epub 2021 Jun 8.

Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA. Electronic address:

We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT05) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cell differentiation. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal. Read More

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Children are safe in schools: a review of the Irish experience of reopening schools during the COVID-19 pandemic.

Public Health 2021 Jun 12;195:158-160. Epub 2021 Jun 12.

Office of the Clinical Director for Health Protection, HSE, Ireland; Department of Public Health, HSE Midlands, Tullamore, Ireland.

Objectives: Schools in the Republic of Ireland reopened to students and staff in late August 2020. We sought to determine the test positivity rate of close contacts of cases of coronavirus disease 2019 (COVID-19) in schools during the first half-term of the 2020/2021 academic year.

Methods: National-level data from the schools' testing pathway were interrogated to determine the positivity rate of close contacts of cases of COVID-19 in Irish primary, postprimary and special schools during the first half-term of 2020/2021 academic year. Read More

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Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia.

Parasitol Int 2021 Jun 12:102404. Epub 2021 Jun 12.

Institute of Veterinary Medicine, Mongolian University of Life Science, Zaisan, 17042, Ulaanbaatar, Mongolia. Electronic address:

Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. Read More

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IN VITRO and IN VIVO TRYPANOCIDAL ACTIVITY OF A BENZOFUROXAN DERIVATIVE AGAINST Trypanosoma cruzi.

Exp Parasitol 2021 Jun 12:108125. Epub 2021 Jun 12.

Department of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria, RS, Brazil.

Chagas disease, caused by Trypanosoma cruzi, is a major public health problem and is described as one of the most neglected diseases worldwide. It affects about 6-7 million people. Currently, only two drugs are available for the treatment of this disease: nifurtimox and benznidazole. Read More

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Validation of the Polish version of the Motivational Postures (Toward Taxes) Questionnaire.

PLoS One 2021 15;16(6):e0252937. Epub 2021 Jun 15.

Centre for Economic Psychology and Decision Sciences, Kozminski University, Warsaw, Poland.

This article presents a Polish adaptation of the Motivational Postures (Towards Taxes) Questionnaire (MPQ). The MPQ is based on the concept of five tax-related motivational postures (Commitment, Capitulation, Resistance, Disengagement and Game Playing) and consists of 29 items. Three studies validating the Polish version of the MPQ are presented. Read More

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Molecular investigation of tick-borne pathogens in ticks removed from tick-bitten humans in the southwestern region of the Republic of Korea.

PLoS One 2021 15;16(6):e0252992. Epub 2021 Jun 15.

Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea.

In this study, we investigated the presence of tick-borne pathogens in ticks removed from tick-bitten humans in the southwestern provinces of the Republic of Korea (ROK). We identified 33 ticks from three tick species, namely Amblyomma testudinarium (60.6%), Haemaphysalis longicornis (27. Read More

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Laboratory evaluation of the rapid diagnostic tests for the detection of Vibrio cholerae O1 using diarrheal samples.

PLoS Negl Trop Dis 2021 Jun 15;15(6):e0009521. Epub 2021 Jun 15.

Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

Background: Cholera, an acute diarrheal disease is a major public health problem in many developing countries. Several rapid diagnostic tests (RDT) are available for the detection of cholera, but their efficacies are not compared in an endemic setting. In this study, we have compared the specificity and sensitivity of three RDT kits for the detection of Vibrio cholerae O1 and compared their efficiency with culture and polymerase chain reaction (PCR) methods. Read More

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Randomness in Cancer Breakpoint Prediction.

J Comput Biol 2021 Jun 15. Epub 2021 Jun 15.

Laboratory of Bioinformatics, Faculty of Computer Science, National Research University Higher School of Economics, Moscow, Russia.

Cancer genomes are susceptible to multiple rearrangements by deleting, inserting, and translocating genomic regions. Recently, the problem of finding determinants of breakpoint formations was approached with machine learning methods; however, unlike cancer point mutations, breakpoint prediction appeared to be a more difficult task, and various machine learning models did not achieve high prediction power often slightly exceeding the threshold of random guessing. This raised the question of whether the breakpoints are random noise in cancer mutagenesis or there exist determinants in structural mutagenesis. Read More

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The Ability of Probiotic Lactobacillus Strains in Removal of Benzo[a]pyrene: a Response Surface Methodology Study.

Probiotics Antimicrob Proteins 2021 Jun 15. Epub 2021 Jun 15.

Department of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

In this study, the ability of various lactic acid bacteria was assessed in removing benzo[a]pyrene (BaP) from contaminated phosphate buffer saline (PBS). Response surface methodology (RSM) was performed using Box-Behnken design to investigate the effect of four independent variables including pH (5-7), incubation time (1-24 h), cell density (10-10 cfu/mL), and initial BaP concentration (5-15 mg/kg) at three levels to evaluate in vitro removal of BaP as response. The results showed that all the tested strains were able to remove BaP from PBS and this reduction was entirely strain-specific. Read More

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Insights into evolution and coexistence of the colibactin- and yersiniabactin secondary metabolite determinants in enterobacterial populations.

Microb Genom 2021 Jun;7(6)

Institute of Hygiene, University of Münster, Münster, Germany.

The bacterial genotoxin colibactin interferes with the eukaryotic cell cycle by causing dsDNA breaks. It has been linked to bacterially induced colorectal cancer in humans. Colibactin is encoded by a 54 kb genomic region in . Read More

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Free-breathing Accelerated Cardiac MRI Using Deep Learning: Validation in Children and Young Adults.

Radiology 2021 Jun 15:202624. Epub 2021 Jun 15.

From the Department of Radiology, Stanford University School of Medicine, 725 Welch Rd, Stanford, CA 94305 (E.J.Z., A.K., S.S.V.); Department of Electrical Engineering, Stanford University, Stanford, Calif (C.M.S.); and Global MR Applications and Workflow, GE Healthcare, Menlo Park, Calif (P.L.).

Background Obtaining ventricular volumetry and mass is key to most cardiac MRI but challenged by long multibreath-hold acquisitions. Purpose To assess the image quality and performance of a highly accelerated, free-breathing, two-dimensional cine cardiac MRI sequence incorporating deep learning (DL) reconstruction compared with reference standard balanced steady-state free precession (bSSFP). Materials and Methods A DL algorithm was developed to reconstruct custom 12-fold accelerated bSSFP cardiac MRI cine images from coil sensitivity maps using 15 iterations of separable three-dimensional convolutions and data consistency steps. Read More

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The Fluorescent Dye 1,6-Diphenyl-1,3,5-hexatriene Binds to Amyloid Fibrils Formed by Human Amylin and Provides a New Probe of Amylin Amyloid Kinetics.

Biochemistry 2021 Jun 15. Epub 2021 Jun 15.

Graduate Program in Biochemistry and Structural Biology, Stony Brook University, Stony Brook, New York 11794, United States.

The fluorescent dye 1,6-diphenyl-1,3,5-hexatriene (DPH) is widely used as a probe of membrane order. We show that DPH also interacts with amyloid fibrils formed by human amylin (h-amylin, also known as islet amyloid polypeptide) in solution, and this results in a 100-fold increase in DPH fluorescence for a sample of 20 μM h-amylin and 0.25 μM DPH. Read More

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Digital reminiscence app co-created by people living with dementia and carers: Usability and eye gaze analysis.

Health Expect 2021 Jun 15. Epub 2021 Jun 15.

Ulster University, Newtownabbey, UK.

Background: This research reports on a pilot study that examined the usability of a reminiscence app called 'InspireD' using eye tracking technology. The InspireD app is a bespoke digital intervention aimed at supporting personalized reminiscence for people living with dementia and their carers. The app was developed and refined in two co-creation workshops and subsequently tested in a third workshop using eye tracking technology. Read More

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Development and preliminary validation of the patient-reported Chronic Itch Burden Scale, assessing health-related quality of life in chronic pruritus.

Br J Dermatol 2021 Jun 15. Epub 2021 Jun 15.

Univ Brest, LIEN and University Hospital of Brest, Department of Dermatology, 2 avenue Foch, F-29200, Brest, France.

Background: Chronic pruritus (CP) significantly affects patients' health-related quality of life (HRQoL). Very few self-reported HRQoL questionnaires exploring CP were developed according to international guidelines thus limiting their use in pre-authorization trials.

Objectives: To develop a self-reported HRQoL questionnaire in patients with CP due to psoriasis, atopic dermatitis, seborrheic dermatitis of the scalp or idiopathic dermatitis; to explore its preliminary psychometric properties. Read More

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Effects of extended abstinence on cognitive functions in tramadol-dependent patients: A cohort study.

Neuropsychopharmacol Rep 2021 Jun 15. Epub 2021 Jun 15.

Department of Psychiatry, Assiut University, Assiut, Egypt.

Background: Some pieces of the literature report impaired cognitive functioning in tramadol dependence. Whether extended abstinence improves cognitive functioning or not is not well studied.

Aim: We aimed to measure the change in cognitive functioning following complete abstinence among individuals with tramadol dependence. Read More

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The effect of source claims on statement believability and speaker accountability.

Mem Cognit 2021 Jun 14. Epub 2021 Jun 14.

Department of Cognitive Science, Central European University, Budapest, Hungary.

What is the effect of source claims (such as "I saw it" or "Somebody told me") on the believability of statements, and what mechanisms are responsible for this effect? In this study, we tested the idea that source claims impact statement believability by modulating the extent to which a speaker is perceived to be committed to (and thereby accountable for) the truth of her assertion. Across three experiments, we presented participants with statements associated with different source claims, asked them to judge how much they believed the statements, and how much the speaker was responsible if the statement turned out to be false. We found that (1) statement believability predicted speaker accountability independently of a statement's perceived prior likelihood or associated source claim; (2) being associated with a claim to first-hand ("I saw that . Read More

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Do standards of care and early outcomes of periprosthetic fractures change during the COVID-19 pandemic? A multicentre study.

J Orthop Traumatol 2021 Jun 14;22(1):22. Epub 2021 Jun 14.

Department of Orthopaedic Surgery, ASST Papa Giovanni XXIII, Piazza OMS 1, 24127, Bergamo, Italy.

Background: Periprosthetic fractures (PPFs) are a growing matter for orthopaedic surgeons, and patients with PPFs may represent a frail target in the case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The purpose of this study is to investigate whether hospital reorganisations during the most severe phase of the SARS-CoV-2 pandemic affected standards of care and early outcomes of patients treated for PPFs in Northern Italy.

Materials And Methods: Data were retrieved from a multicentre retrospective orthopaedics and traumatology database, including 14 hospitals. Read More

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Targeting hepatocyte growth factor in epithelial-stromal interactions in an in vitro experimental model of human periodontitis.

Odontology 2021 Jun 14. Epub 2021 Jun 14.

Charité - Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Augustenburger Platz 1, 13353, Berlin, Germany.

Periodontitis is a chronic inflammatory disease leading to progressive connective tissue degradation and loss of the tooth-supporting bone. Clinical and experimental studies suggest that hepatocyte growth factor (HGF) is involved in the dysregulated fibroblast-epithelial cell interactions in periodontitis. The aim of this study was to explore effects of HGF to impact fibroblast-induced collagen degradation. Read More

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Outcomes of SARS-CoV-2 Infection in Patients with Chronic Liver Disease and Cirrhosis: a N3C Study.

medRxiv 2021 Jun 7. Epub 2021 Jun 7.

Background And Aims: In patients with chronic liver diseases (CLD) with or without cirrhosis, existing data on the risk of adverse outcomes with SARS-CoV-2 infection have been mixed or have limited generalizability. We used the National COVID Cohort Collaborative (N3C) Data Enclave, a harmonized electronic health record (EHR) dataset of 5.9 million nationally-representative, diverse, and gender-balanced patients, to describe outcomes in patients with CLD and cirrhosis with SARS-CoV-2. Read More

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Rhizosphere Bacterial Networks, but Not Diversity, Are Impacted by Pea-Wheat Intercropping.

Front Microbiol 2021 28;12:674556. Epub 2021 May 28.

Agroécologie, AgroSup Dijon, INRAE, Université de Bourgogne - Université de Bourgogne Franche-Comté, Dijon, France.

Plant-plant associations, notably cereal-legume intercropping, have been proposed in agroecology to better value resources and thus reduce the use of chemical inputs in agriculture. Wheat-pea intercropping allows to decreasing the use of nitrogen fertilization through ecological processes such as niche complementarity and facilitation. Rhizosphere microbial communities may account for these processes, since they play a major role in biogeochemical cycles and impact plant nutrition. Read More

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A genome-wide portrait of pervasive drug contaminants.

Sci Rep 2021 Jun 14;11(1):12487. Epub 2021 Jun 14.

Genome Science & Technology Graduate Program, University of British Columbia, Vancouver, Canada.

Using a validated yeast chemogenomic platform, we characterized the genome-wide effects of several pharmaceutical contaminants, including three N-nitrosamines (NDMA, NDEA and NMBA), two related compounds (DMF and 4NQO) and several of their metabolites. A collection of 4800 non-essential homozygous diploid yeast deletion strains were screened in parallel and the strain abundance was quantified by barcode sequencing. These data were used to rank deletion strains representing genes required for resistance to the compounds to delineate affected cellular pathways and to visualize the global cellular effects of these toxins in an easy-to-use searchable database. Read More

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Cross sectional study of the clinical characteristics of French primary care patients with COVID-19.

Sci Rep 2021 06 14;11(1):12492. Epub 2021 Jun 14.

Primary Care Unit, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

The early identification of patients suffering from SARS-CoV-2 infection in primary care is of outmost importance in the current pandemic. The objective of this study was to describe the clinical characteristics of primary care patients who tested positive for SARS-CoV-2. We conducted a cross-sectional study between March 24 and May 7, 2020, involving consecutive patients undergoing RT-PCR testing in two community-based laboratories in Lyon (France) for a suspicion of COVID-19. Read More

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Application of substitution box of present cipher for automated detection of snoring sounds.

Artif Intell Med 2021 Jul 6;117:102085. Epub 2021 May 6.

Ngee Ann Polytechnic, Department of Electronics and Computer Engineering, 599489, Singapore; Department of Biomedical Engineering, School of Science and Technology, SUSS University, Singapore; Department of Biomedical Informatics and Medical Engineering, Asia University, Taichung, Taiwan.

Background And Purpose: Snoring is one of the sleep disorders, and snoring sounds have been used to diagnose many sleep-related diseases. However, the snoring sound classification is done manually which is time-consuming and prone to human errors. An automated snoring sound classification model is proposed to overcome these problems. Read More

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Deep ensemble multitask classification of emergency medical call incidents combining multimodal data improves emergency medical dispatch.

Artif Intell Med 2021 Jul 13;117:102088. Epub 2021 May 13.

Biomedical Data Science Laboratory (BDSLab), Instituto de Aplicaciones de las Tecnologías de la Información y de las Comunicaciones Avanzadas (ITACA), Universitat Politècnica de València (UPV), Camino de Vera s/n, 46022 Valencia, Spain.

The objective of this work was to develop a predictive model to aid non-clinical dispatchers to classify emergency medical call incidents by their life-threatening level (yes/no), admissible response delay (undelayable, minutes, hours, days) and emergency system jurisdiction (emergency system/primary care) in real time. We used a total of 1 244 624 independent incidents from the Valencian emergency medical dispatch service in Spain, compiled in retrospective from 2009 to 2012, including clinical features, demographics, circumstantial factors and free text dispatcher observations. Based on them, we designed and developed DeepEMC, a deep ensemble multitask model integrating four subnetworks: three specialized to context, clinical and text data, respectively, and another to ensemble the former. Read More

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Tacrolimus-loaded chitosan nanoparticles for enhanced skin deposition and management of plaque psoriasis.

Carbohydr Polym 2021 Sep 23;268:118238. Epub 2021 May 23.

Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address:

Tacrolimus is a natural macrolide that exhibits an anti-proliferative action by T-lymphocytic cells inhibition. Hence, it was tested as a potential topical treatment to improve and control psoriatic plaques. In this study, for the first time the lipophilic tacrolimus in chitosan nanoparticles was used to achieve the desired response and dermal retention of the drug using a modified ionic gelation technique. Read More

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September 2021

A Novel Real-Time PCR Assay for Detection of HLA-A*31:01 in Individuals Being Considered for Carbamazepine Therapy.

CNS Spectr 2021 Apr;26(2):154-155

Genomind, Inc., King of Prussia, PA, USA.

Background: Carbamazepine, an anticonvulsant also used as a mood stabilizer and for trigeminal neuralgia, is associated with serious, sometimes fatal cutaneous adverse drug reactions, including Stevens Johnson Syndrome and toxic epidermal necrolysis1. Current literature demonstrates a genetic predisposition linked to specific class I and II human leukocyte antigen (HLA) types in various ethnic populations2. HLA-A*31:01 is one such HLA type, and is routinely identified by the tag SNP rs1061235. Read More

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Economic Burden of Dementia Related Psychosis Among Medicare Beneficiaries: A State-Transition Markov Analysis of Total Annual Direct Costs.

CNS Spectr 2021 Apr;26(2):161

An-L-It-Iks, Inc., Framingham, MA, USA.

Study Objective: Dementia related psychosis (DRP), characterized by debilitating symptoms such as hallucinations and delusions, is estimated to affect 2.4M people with dementia in the US. Patients with DRP may have twice the rate of dementia progression compared to patients with no DRP. Read More

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Acroflavone A, a new prenylated flavone from the fruit of (L.) Miq.

Nat Prod Res 2021 Jun 15:1-7. Epub 2021 Jun 15.

Faculty of Science, Department of Chemistry, and Center for Innovation in Chemistry, Khon Kaen University, Khon Kaen, Thailand.

Phytochemical investigation of the fruit of (L.) Miq. led to the isolation of a new prenylated flavone, acroflavone A (), together with eight known compounds (-). Read More

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Computed Tomography Angiography Spot Sign, Hematoma Expansion, and Functional Outcome in Spontaneous Cerebellar Intracerebral Hemorrhage.

Stroke 2021 Jun 15:STROKEAHA120033297. Epub 2021 Jun 15.

Department of Neurology and Neurosurgery, Brain Center Rudolf Magnus, University Medical Center Utrecht, the Netherlands (S.D.S., J.R.S., G.J.E.R., H.B.B.).

Background And Purpose: The computed tomography angiography spot sign is associated with hematoma expansion, case fatality, and poor functional outcome in spontaneous supratentorial intracerebral hemorrhage (ICH). However, no data are available on the spot sign in spontaneous cerebellar ICH.

Methods: We investigated consecutive patients with spontaneous cerebellar ICH at 3 academic hospitals between 2002 and 2017. Read More

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