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Efficacy of Urine Dipstick Test in Diagnosing Urinary Tract Infection and Detection of the blaCTX-M Gene among ESBL-Producing .

Diseases 2021 Aug 27;9(3). Epub 2021 Aug 27.

Central Department of Microbiology, Tribhuvan University, Kirtipur 44600, Kathmandu, Nepal.

A urine dipstick test used for prompt diagnosis of urinary tract infection (UTI) is a rapid and cost-effective method. The main objective of this study was to compare the efficacy of the urine dipstick test with culture methods in screening for UTIs along with the detection of the gene in extended spectrum β-lactamase (ESBL)-producing . A total of 217 mid-stream urine samples were collected from UTI-suspected patients attending Bharatpur Hospital, Chitwan, and tested by dipstick test strip (COMBI-10SL, Germany) prior to the culture. Read More

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Mediating Role of Moral Disengagement between Emotional Manipulation and Psychological Well-Being: Does Age Matter?

Behav Sci (Basel) 2021 Aug 31;11(9). Epub 2021 Aug 31.

National Institute of Psychology, Quaid i Azam University, Islamabad 45401, Pakistan.

: When people hone their emotional skills, they become better at manipulating others. They use their emotional skills for coping with the demands of life. This study investigated the mediating role of moral disengagement between emotional manipulation and psychological well-being. Read More

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Adverse childhood experiences amplify the longitudinal associations of adult daily stress and health.

Child Abuse Negl 2021 Sep 21;122:105337. Epub 2021 Sep 21.

Human Development and Family Studies, The Pennsylvania State University, University Park, PA 16802, United States of America.

Background And Objective: The long-term negative impact of Adverse Childhood Experiences (ACEs) is now well-recognized; however, little research has explored the link between ACEs and daily stress processes in adulthood. The current study aimed to examine the effect of ACEs in the association between daily stressor exposure and daily negative affect, and whether such associations would predict long-term health and well-being.

Methods: Using data from the National Study of Daily Experiences 2 (NSDE 2) and the Midlife in the United States (MIDUS) studies, multilevel moderated mediation analyses were conducted to account for daily measurements nested within individuals. Read More

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September 2021

Increasing diagnostic possibilities using the geneLEAD VIII platform for detection of SARS-CoV-2.

J Virol Methods 2021 Sep 22:114291. Epub 2021 Sep 22.

Department of Medical Microbiology, Leiden University Medical Center, 2300 RC, Leiden, the Netherlands.

At the time SARS-CoV-2 was identified as the cause of coronavirus disease 2019 (COVID-19) no in vitro diagnostic (IVD) tests were available since it was a new virus. Very shortly after the release of the genomic sequence of SARS-CoV-2, laboratory-developed tests (LDTs) were developed, made available and implemented in several laboratories in the Netherlands and globally. In this study, the performance of an E-gene Sarbeco specific real-time reverse-transcriptase PCR (RT-PCR) was verified on the open modus of the geneLEAD VIII sample-to-answer platform. Read More

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September 2021

Syndromic approach to SARS-CoV-2 detection using QIAstat-Dx SARS-CoV-2 Panel from clinical samples.

J Virol Methods 2021 Sep 22:114300. Epub 2021 Sep 22.

Department of Clinical Virology, Institute of Liver & Biliary Sciences, New Delhi, India. Electronic address:

The QIAstat-Dx SARS-CoV-2 panel is a multiplex cartridge based assay based on real time PCR which can detect 17 respiratory viruses, including the novel coronavirus SARS-CoV-2. A syndromic approach is the need of the hour for COVID-19 diagnostics among patients presenting with respiratory symptoms. The present study was done to evaluate 120 archived respiratory clinical specimens for SARS-CoV-2 on the SARS-CoV-2 panel. Read More

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September 2021

A Simplified Adult Expiratory Support Device: First Clinical Cases in a Low Resource Setting.

Ann Thorac Surg 2021 Sep 22. Epub 2021 Sep 22.

Division of Critical Care Medicine, Department of Anaesthesia, Critical Care and Pain Medicine at Boston Children's Hospital.

Purpose: In Low and Middle-Income Countries mechanical ventilators or commercially available devices used to offer continuous positive airway pressure (CPAP) are not readily affordable and available. In Ghana, nearly 10% of critically ill patients presenting to the emergency department require ventilator support.

Description: We designed, built and tested a simple expiratory positive airway pressure (EPAP) device to provide adult respiratory support in low resource environments with or without supplemental oxygen and without the need for electricity. Read More

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September 2021

Clinical performance of methylation as a biomarker for cervical carcinoma-in-situ and cancer diagnosis: a worldwide study.

Int J Cancer 2021 Sep 25. Epub 2021 Sep 25.

Centre for Cancer Prevention, Wolfson Institute of Population Health Queen Mary University of London, Charterhouse Square, London, United Kingdom.

The shift towards primary HPV-based screening has necessitated the search for a secondary triage test that provides sufficient sensitivity to detect high grade cervical intraepithelial neoplasia (CIN) and cancer, but also brings an improved specificity to avoid unnecessary clinical work and colposcopy referrals. We evaluated the performance of the previously described DNA-methylation test (S5) in detecting CIN3 and cancers from diverse geographic settings in high, medium and low income countries, using the cut-off of 0.80 and exploratory cut-offs of 2. Read More

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September 2021

VACuum INtubation (VACcIN) box restricts the exhaled air dispersion generated by simulated cough: description and simulation-based tests of an innovative aerosolization protective prototype.

J Clin Monit Comput 2021 Sep 25. Epub 2021 Sep 25.

Department of Anesthesiology and Pain Medicine of Maisonneuve-Rosemont Hospital, CIUSSS de l'Est de l'Ile de Montreal, University of Montreal, 5415 Boulevard de l'Assomption, Montreal, QC, H1T 2M4, Canada.

The COVID-19 pandemic has caused personal protective equipment shortages worldwide and required healthcare workers to develop novel ways of protecting themselves. Anesthesiologists in particular are exposed to increased risks of contamination when performing interventions such as airway manipulations. We developed and tested an aerosolization protective device which contains aerosols around the patient's airway and helps eliminate particles using negative pressure. Read More

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September 2021

A prospective observational study of persistent opioid use after complex foot and ankle surgery.

Can J Anaesth 2021 Sep 24. Epub 2021 Sep 24.

Department of Anesthesiology and Pain Medicine, University of Alberta, Edmonton, AB, Canada.

Purpose: The incidence of persistent postsurgical opioid use (PPOU) after complex foot and ankle surgery is unknown. We aimed to determine the incidence and characteristics of PPOU in opioid-naïve, occasional, and regular opioid users at baseline and at six weeks, three months, and six months postoperatively.

Methods: We conducted a prospective observational study in patients undergoing complex foot and ankle surgery over an 18-month period. Read More

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September 2021

Mutual inhibition effect of sulfur compounds in the hydrodesulfurization of thiophene, 2-ethylthiophene and benzothiophene ternary mixture.

Sci Rep 2021 Sep 24;11(1):19053. Epub 2021 Sep 24.

Petroleum-Gas University of Ploiești, Bucharest Blvd. 39, 100680, Ploiești, Romania.

The hydrodesulphurization of a model component and equimolar ternary mixture of thiophene, 2-ethylthiophene and benzothiophene over sulphided CoMo/γ-AlO and CoMo/γ-AlO-NbO catalysts were investigated in a fixed bed flow reactor. The catalysts were prepared by incipient wetness impregnation method and characterized by textural characteristics, total acidity and chemical species present on the catalysts surface. The characterization results showed that both CoMo/γ-AlO-NbO and sulphided CoMo/γ-AlO catalysts exhibit a mesoporous structure with cylindrical pores open at both ends, evidenced by the IV type adsorption-desorption isotherm with a H1 hysteresis loop and have an average pore diameter between 3 and 4 nm. Read More

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September 2021

If We Make It, Will They Take It? Attitudes Towards the Acceptability of Chlamydia Point-of-Care Testing Among Adolescents and Young Adults.

Sex Transm Dis 2021 Sep 22. Epub 2021 Sep 22.

Department of Pediatrics, Division of Adolescent Medicine Division of Pediatric Infectious Diseases, SUNY Downstate Health Sciences University, Brooklyn, NY.

Introduction: Adolescent women, 15-19 years of age, have the highest rate of Chlamydia trachomatis infection in the US. The objective of this study was to ascertain knowledge, experience, of C. trachomatis and acceptance of C. Read More

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September 2021

Lumbar Spine Surgery and What We Lost in the Era of the Coronavirus Pandemic: A Survey of the Lumbar Spine Research Society.

Clin Spine Surg 2021 Sep 21. Epub 2021 Sep 21.

Department of Neurosurgery Stephens Family Clinical Research Institute, Carle Foundation Hospital, Urbana, IL Hackensack Meridian School of Medicine, Nutley Mountainside Medical Center, Montclair NJ Rothman Orthopaedic Institute, Philadelphia, PA Department of Electrical and Computer Engineering, The Grainger College of Engineering, Urbana, IL Department of Neurosurgery, Thomas Jefferson University, Philadelphia, PA.

Study Design: This was a survey of the surgeon members of the Lumbar Spine Research Society (LSRS).

Objective: The purpose of this study was to assess trends in surgical practice and patient management involving elective and emergency surgery in the early months of the coronavirus pandemic.

Summary Of Background Data: The novel coronavirus has radically disrupted medical care in the first half of 2020. Read More

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September 2021

β-arrestin-biased allosteric modulator potentiates Carvedilol stimulated β adrenergic receptor cardioprotection.

Mol Pharmacol 2021 Sep 24. Epub 2021 Sep 24.

Medicine, Duke University, United States

β adrenergic receptors (βARs) are central regulators of cardiac function and a drug target for cardiac disease. As a member of G protein-coupled receptor family, βARs activate cellular signaling by primarily coupling to Gs proteins to activate adenylyl cyclase and cAMP-dependent pathways, and the multifunctional adaptor-transducer protein β-arrestin. Carvedilol, a traditional β-blocker widely used in treating high blood pressure and heart failure by blocking βAR-mediated G-protein activation, can selectively stimulate Gs-independent β-arrestin signaling of βARs, a process known as β-arrestin-biased agonism. Read More

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September 2021

Serological responses to SARS-CoV-2 following non-hospitalised infection: clinical and ethnodemographic features associated with the magnitude of the antibody response.

BMJ Open Respir Res 2021 Sep;8(1)

Department of Clinical Immunology Service, University of Birmingham College of Medical and Dental Sciences, Birmingham, UK

Objective: To determine clinical and ethnodemographic correlates of serological responses against the SARS-CoV-2 spike glycoprotein following mild-to-moderate COVID-19.

Design: A retrospective cohort study of healthcare workers who had self-isolated due to COVID-19.

Setting: University Hospitals Birmingham NHS Foundation Trust, UK (UHBFT). Read More

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September 2021

A Comprehensive Clinicopathologic and Molecular Study of 19 Primary Effusion Lymphomas in HIV-infected Patients.

Am J Surg Pathol 2021 Sep 23. Epub 2021 Sep 23.

Departments of Pathology Clinical Immunology Hematological Cytogenetics Laboratory Hematology Laboratory, Saint-Louis Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP) University of Paris, Paris Department of Pathology, Henri Mondor Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP) INSERM U955, University Paris-Est Créteil, Créteil, France.

Primary effusion lymphoma (PEL) is associated with human herpesvirus 8 and frequently with Epstein-Barr virus (EBV). We report here a single-center series of 19 human immunodeficiency virus-associated PELs, including 14 EBV+ and 5 EBV- PELs. The objectives were to describe the clinicopathologic features of PELs, with a focus on programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) expression, to search for genetic alterations by targeted deep sequencing analysis, and to compare the features between EBV+ and EBV- cases. Read More

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September 2021

Pre-game drinking among young adults and its association with positive and negative alcohol consequences.

Addict Behav 2021 Sep 15;124:107120. Epub 2021 Sep 15.

Pennsylvania State University, Edna Bennett Pierce Prevention Research Center, 320E Biobehavioral Health Building, University Park, PA 16802, United States.

Background: Pre-gaming, or drinking before going out, is common among young adults and associated with heavier drinking and negative consequences. However, findings have been mixed as to whether a unique, day-level association between pre-gaming and negative consequences exists independent of alcohol intake. It is also unknown whether young adults experience more positive consequences of alcohol use on days they engage in pre-gaming. Read More

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September 2021

Epigenetic aging biomarkers and occupational exposure to benzene, trichloroethylene and formaldehyde.

Environ Int 2021 Sep 21;158:106871. Epub 2021 Sep 21.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, 9609 Medical Center Drive, Rockville, MD 20850, USA.

Epigenetic aging biomarkers are associated with increased morbidity and mortality. We evaluated if occupational exposure to three established chemical carcinogens is associated with acceleration of epigenetic aging. We studied workers in China occupationally exposed to benzene, trichloroethylene (TCE) or formaldehyde by measuring personal air exposures prior to blood collection. Read More

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September 2021

A simple pooling salivary test for SARS-CoV-2 diagnosis: a Columbus' egg?

Virus Res 2021 Sep 21:198575. Epub 2021 Sep 21.

Dental Surgeon, Bari 70010, Italy. Electronic address:

Saliva is an appropriate specimen for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) diagnosis. The possibility of pooling samples of saliva, using non-invasive bibula strips for sampling, was explored employing Bovine coronavirus (BCoV) spiked saliva. In laboratory, up to 30 saliva-soaked strips were pooled in a single tube with 2mL of medium. Read More

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September 2021

Preliminary Study of Virtual-reality-guided Meditation for Veterans with Stress and Chronic Pain.

Altern Ther Health Med 2021 Sep 24. Epub 2021 Sep 24.

Context: Studies have found evidence for meditation's positive effects on health and well-being, but the difficulty of learning and engaging in meditation practice has been identified as a major barrier. Virtual reality (VR) technology may facilitate meditation practice by immersing users in a distraction-free and calming virtual environment, although this theory has yet to be rigorously tested.

Objective: This study intended to examine the efficacy of VR-guided meditation in a population of US veterans as a tool to facilitate meditation and relaxation practice for reduction of stress and chronic pain as well as to elicit participants' feedback regarding their perceptions of VR-guided meditation. Read More

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September 2021

Analyses of Original and Computationally-Derived Electronic Health Record Data: The National COVID Cohort Collaborative.

J Med Internet Res 2021 Sep 12. Epub 2021 Sep 12.

Department of Biomedical and Medical Education, School of Medicine, University of Washington, Seattle, US.

Background: Computationally-derived ("synthetic") data can enable the creation and analysis of clinical, laboratory, and diagnostic data as if they were the original electronic health record (EHR) data. Synthetic data can support data sharing to answer critical research questions to address the COVID-19 pandemic.

Objective: We aimed to (1) compare the results from analyses of synthetic data to those from original data, and (2) assess the strengths and limitations of leveraging computationally-derived data for research purposes. Read More

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September 2021

A novel, eco-friendly bio-nanocomposite (Alg-Cst/Kal) for the adsorptive removal of crystal violet dye from its aqueous solutions.

Int J Phytoremediation 2021 Sep 24:1-12. Epub 2021 Sep 24.

Department of Chemistry, Maulana Azad National Institute of Technology, Bhopal, India.

Herein, we report the synthesis of a novel bio-nanocomposite (Alg-Cst/Kal) for the effective removal of the dye "Crystal Violet" from its aqueous solutions. In order to observe the surface morphology and functional groups, the bio-nanocomposite was characterized using various techniques such as SEM, EDX, TEM, FTIR, XRD, and TGA. The effect of parameters like contact time, pH, concentration and temperature on the adsorption of the dye over adsorbent has been studied in detail. Read More

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September 2021

Head and Neck Low-Grade Fibromyxoid Sarcoma: A Clinicopathologic Study of 15 Cases.

Head Neck Pathol 2021 Sep 24. Epub 2021 Sep 24.

Department of Pathology and Laboratory Medicine, Aga Khan University Hospital, Karachi, Pakistan.

Low-grade fibromyxoid sarcoma (LGFMS) is an uncommon mesenchymal tumor usually arising in the lower extremities and trunk. Only rare examples in the head and neck region have been reported. Fifteen cases of head and neck LGFMS were retrieved. Read More

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September 2021

PET/CT imaging of head-and-neck and pancreatic cancer in humans by targeting the "Cancer Integrin" αvβ6 with Ga-68-Trivehexin.

Eur J Nucl Med Mol Imaging 2021 Sep 24. Epub 2021 Sep 24.

Institute of Pathology, Technische Universität München, Munich, Germany.

Purpose: To develop a new probe for the αvβ6-integrin and assess its potential for PET imaging of carcinomas.

Methods: Ga-68-Trivehexin was synthesized by trimerization of the optimized αvβ6-integrin selective cyclic nonapeptide Tyr2 (sequence: c[YRGDLAYp(NMe)K]) on the TRAP chelator core, followed by automated labeling with Ga-68. The tracer was characterized by ELISA for activities towards integrin subtypes αvβ6, αvβ8, αvβ3, and α5β1, as well as by cell binding assays on H2009 (αvβ6-positive) and MDA-MB-231 (αvβ6-negative) cells. Read More

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September 2021

Longitudinal Pathways to Influenza Vaccination Vary With Socio-Structural Disadvantages.

Ann Behav Med 2021 Sep 24. Epub 2021 Sep 24.

University of Illinois at Urbana-Champaign, Champaign, IL 61820, USA.

Background: Although influenza vaccination can prevent influenza-related deaths, uptake remains low, particularly in disadvantaged populations.

Purpose: A theoretical model of psychological pathways to vaccination accounting for the direct and moderating role of socio-structural factors was tested. The study sought to understand the joint contributions of psychological (i. Read More

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September 2021

Influence of the Amount of Fresh Specimen on the Isolation of Tumor Mesenchymal Stem-Like Cells from High-Grade Glioma.

Yonsei Med J 2021 Oct;62(10):936-942

Yonsei University College of Medicine, Seoul, Korea.

Purpose: A critical indicator of the overall survival of patients with high-grade glioma is the successful isolation of tumor mesenchymal stem-like cells (tMSLCs), which play important roles in glioma progression. However, attempts to isolate tMSLCs from surgical specimens have not always been successful, and the reasons for this remain unclear. Considering that the amount of surgical high-grade glioma specimens varies, we hypothesized that larger surgical specimens would be better for tMSLC isolation. Read More

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October 2021

Preliminary findings of performing aerosol generating procedures using a novel innovative mask in times of COVID-19 pandemic.

Med J Malaysia 2021 Aug;76(Suppl 4):20-22

Universiti Malaysia Sabah, 3Faculty of Engineering, Kota Kinabalu, Sabah, Malaysia.

This article describes an innovative mask consisting of a newly fabricated 3-ply surgical face mask with a custom made attachment consisting of a plastic dome and a oneway valve port that allows endoscopes to be inserted through it. The mask was tested in-vitro with simulated sneezing using fluorescent dyes and also received positive feedbacks from field tests of 30 masks on real users in different hospitals. This innovative mask is useful in providing extra barrier for endoscopic procedures in ENT and can be used beyond this pandemic in patients with other infectious diseases. Read More

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A major outbreak of COVID-19 at aresidential care home.

Dan Med J 2021 Sep 23;68(10). Epub 2021 Sep 23.

Introduction SARS-CoV-2 outbreaks at care homes are associated with a high morbidity and mortality. We aimed to study the molecular epidemiology of a major care home outbreak in Denmark. Methods After a staff member had been tested positive on 16 November 2020, a bundle approach programme was initiated including frequent surveillance screenings of residents and staff, isolation and cohorting procedures. Read More

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September 2021

Spotlight influenza: Extending influenza surveillance to detect non-influenza respiratory viruses of public health relevance: analysis of surveillance data, Belgium, 2015 to 2019.

Euro Surveill 2021 09;26(38)

National Influenza Centre, Sciensano, Brussels, Belgium.

BackgroundSeasonal influenza-like illness (ILI) affects millions of people yearly. Severe acute respiratory infections (SARI), mainly influenza, are a leading cause of hospitalisation and mortality. Increasing evidence indicates that non-influenza respiratory viruses (NIRV) also contribute to the burden of SARI. Read More

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September 2021

Prevalence of spp. in symptomatic BaAka Pygmies inhabiting the rural Dzanga Sangha region of the Central African Republic.

Ann Agric Environ Med 2021 Sep 7;28(3):483-490. Epub 2021 Sep 7.

Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine, Medical University, Gdańsk, Poland.

Introduction: Malaria remains a diagnostic and therapeutic challenge in many endemic regions of sub-Saharan Africa. It is one of the most important causes of morbidity and mortality, especially in children <5 years. is responsible for the majority of severe malaria cases in sub-Saharan Africa, but is not the exclusive one. Read More

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September 2021

Role of mass spectrometry in the study of interactions between amylin and metal ions.

Mass Spectrom Rev 2021 Sep 24. Epub 2021 Sep 24.

Department of Medicine, University of Padova, Padova, Italy.

Amylin (islet amyloid polypeptide [IAPP]) is a neuroendocrine hormone synthesized with insulin in the beta cells of pancreatic islets. The two hormones act in different ways: in fact insulin triggers glucose uptake in muscle and liver cells, removing glucose from the bloodstream and making it available for energy use and storage, while amylin regulates glucose homeostasis. Aside these positive physiological aspects, human amyloid polypeptide (hIAPP) readily forms amyloid in vitro. Read More

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September 2021