26,989 results match your criteria terminally


Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity.

Genes Cells 2021 May 7. Epub 2021 May 7.

Department of Pathology, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan.

Mesenchymal stem cells (MSCs) are the likely precursors of multiple lines of mesenchymal cells. The existence of bona fide MSCs with self-renewal capacity and differentiation potential into all mesenchymal lineages, however, has been unclear because of the lack of MSC-specific marker(s) that are not expressed by the terminally differentiated progeny. Meflin, a glycosylphosphatidylinositol-anchored protein, is an MSC marker candidate that is specifically expressed in rare stromal cells in all tissues. Read More

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Assessing hospitalized patients' quality of life from external indices: the perspectives of lay people and health professionals.

Qual Life Res 2021 May 6. Epub 2021 May 6.

Institute of Advanced Studies (EPHE), Paris, France.

Purpose: We examined the way people assess hospitalized patients' quality of life from what they immediately observe when entering the patient's room, from what they learn by conversing with the patient, and from what they know about the patient's social life.

Methods: A sample of 474 adults (among them 7 physicians, 57 nurses, and 42 nurse's aides) aged 18-90 years was presented with 54 realistic scenarios depicting the situation of a terminally ill patient, and created by orthogonally combining the levels of four factors: chronic pain (e.g. Read More

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Twenty-five years after Dolly - How far have we come?

Reproduction 2021 May 1. Epub 2021 May 1.

A Schnieke, Department of Animal Sciences, Technical University of Munich School of Life Sciences Weihenstephan, Freising, Germany.

For more than a century the scientific consensus stated that a nucleus from a terminally differentiated cell would not be able to control the development of offspring. This theory was refuted by the birth of Dolly, the first animal generated by nuclear transfer using an adult somatic cell as nuclear donor. Following this paradigm shift, a wide variety of animals has been cloned using somatic cell nuclear transfer. Read More

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Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications.

Methods Mol Biol 2021 May 5. Epub 2021 May 5.

Jeffrey Cheah Biomedical Centre, Department of Surgery, Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Long-term neuroepithelial-like stem cells (lt-NES) derived from human embryonic stem cells are a stable self-renewing progenitor population with high neurogenic potential and phenotypic plasticity. Lt-NES are amenable to regional patterning toward neurons and glia subtypes and thus represent a valuable source of cells for many biomedical applications. For use in regenerative medicine and cell therapy, lt-NES and their progeny require derivation with high-quality culture conditions suitable for clinical use. Read More

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Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils.

Cell Rep Med 2021 Apr 8;2(4):100239. Epub 2021 Apr 8.

Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA.

Neutrophils are often considered terminally differentiated and poised for bacterial killing. In chronic diseases such as cystic fibrosis (CF), an unexplained paradox pits massive neutrophil presence against prolonged bacterial infections. Here, we show that neutrophils recruited to CF airways and in an transmigration model display rapid and broad transcriptional firing, leading to an upregulation of anabolic genes and a downregulation of antimicrobial genes. Read More

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Assessment of Burden and Stress Among Caregivers of Terminally Ill Patients in a Saudi University Hospital: A Cross-Sectional Study.

Cureus 2021 Mar 31;13(3):e14215. Epub 2021 Mar 31.

Oncology Center, King Abdullah University Hospital, Jeddah, SAU.

Background As the burden of cancer in Saudi Arabia has increased, the number of terminally ill patients is growing. In parallel, family caregivers' burden has emerged as an escalating problem within the field of palliative medicine. In this study, we aimed to explore the prevalence and types of burden experienced by caregivers of terminally ill patients and the associated risk factors. Read More

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Single-keratinocyte transcriptomic analyses identify different clonal types and proliferative potential mediated by FOXM1 in human epidermal stem cells.

Nat Commun 2021 05 4;12(1):2505. Epub 2021 May 4.

Centre for Regenerative Medicine "Stefano Ferrari", University of Modena and Reggio Emilia, Modena, Italy.

Autologous epidermal cultures restore a functional epidermis on burned patients. Transgenic epidermal grafts do so also in genetic skin diseases such as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells, which can be only partially distinguished from the other clonogenic keratinocytes and cannot be prospectively isolated. Read More

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Cultural implications for disclosure of diagnosis and prognosis toward terminally ill cancer patients in China: A literature review.

Palliat Support Care 2021 May 5:1-7. Epub 2021 May 5.

Faculty of Medicine, Department of Palliative Care, RWTH Aachen University, Aachen, Germany.

Objective: Health professionals in China tend to avoid open communication with terminally ill cancer patients concerning their diagnosis and prognosis. This review aims to explore Chinese cultural beliefs and attitudes concerning disclosure and death among health professionals and cancer patients in China and to investigate preferences of terminally ill cancer patients for a "good death."

Method: A narrative literature review was conducted in May 2020 on MEDLINE, EMBASE, and WEB OF SCIENCE to include all studies with clear study design which presented its own study data or referred to data within underlying studies, published between January 2000 and May 2020, having cancer patients and/or healthcare professionals as participants, conducted in Mainland China, Hong Kong, or Taiwan and containing relevant data concerning "medical disclosure" or "good death. Read More

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Changing the eligibility criteria for welfare payments at the end of life - a budget impact analysis for England and Wales.

BMC Health Serv Res 2021 May 4;21(1):421. Epub 2021 May 4.

Health Sciences School, The University of Sheffield, Barber House Annexe, 387 Glossop Road, Sheffield, S10 2HQ, UK.

Background: Terminal illness can cause a financial burden for many households. In England and Wales, fast-track access to welfare payments is available through special rules for the terminally ill (SRTI). Individuals are eligible for SRTI if they are judged to have 6 months or less to live. Read More

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Chronic active non-lethal human-type tuberculosis in a high royal Bavarian officer of Napoleonic times-a mummy study.

PLoS One 2021 4;16(5):e0249955. Epub 2021 May 4.

Institute of Legal Medicine, Ludwig-Maximilians University (LMU), Munich, Germany.

In paleopathology, morphological and molecular evidence for infection by mycobacteria of the M. tuberculosis complex (MTC) is frequently associated with early death. In the present report, we describe a multidisciplinary study of a well-preserved mummy from Napoleonic times with a long-standing tuberculous infection by M. Read More

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DNA damage and repair in differentiation of stem cells and cells of connective cell lineages: A trigger or a complication?

Eur J Histochem 2021 May 3;65(2). Epub 2021 May 3.

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The review summarizes literature data on the role of DNA breaks and DNA repair in differentiation of pluripotent stem cells (PSC) and connective cell lineages. PSC, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC), are rapidly dividing cells with highly active DNA damage response (DDR) mechanisms to ensure the stability and integrity of the DNA. In PSCs, the most common DDR mechanism is error-free homologous recombination (HR) that is primarily active during S phase of the cell cycle, whereas in quiescent, slow-dividing or non-dividing tissue progenitors and terminally differentiated cells, error-prone non-homologous end joining (NHEJ) mechanism of the double-strand break (DSB) repair is dominating. Read More

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Diversity in Representations and Voices of Terminally Ill People in End-of-Life Documentaries.

Authors:
Outi J Hakola

J Palliat Care 2021 May 3:8258597211013961. Epub 2021 May 3.

Area and Cultural Studies, University of Helsinki, Helsinki, Finland.

Introduction: The 21st century has seen a proliferation of end-of-life documentary films and television documentaries that contribute to building a public image of hospice and palliative care. The way in which terminally ill patients are represented in these documentaries creates impressions of who is welcomed to receive end-of-life care. These documentary representations have not been previously mapped. Read More

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T cell plasticity in renal autoimmune disease.

Cell Tissue Res 2021 May 3. Epub 2021 May 3.

I. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

The presence of immune cells is a morphological hallmark of rapidly progressive glomerulonephritis, a disease group that includes anti-glomerular basement membrane glomerulonephritis, lupus nephritis, and anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis. The cellular infiltrates include cells from both the innate and the adaptive immune responses. The latter includes CD4 and CD8 T cells. Read More

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N-terminally endogenous mKate2-tagged HIM-4 in .

Authors:
Shinji Ihara

MicroPubl Biol 2021 Apr 29;2021. Epub 2021 Apr 29.

Faculty of Life and Environmental, Prefectural University of Hiroshima, Hiroshima, Japan.

The gene encodes the homolog of hemicentin, an evolutionarily conserved extracellular matrix protein. Despite being an extracellular matrix protein, mutations in him-4 result in pleiotropic defects during development and demonstrate tissue fragility. While previous studies in have confirmed the localization of either transgenic HIM-4::GFP or endogenous C-terminally tagged HIM-4::mNeonGreen, only green or green/yellow fluorescent protein-tagged HIM-4 are available to the C. Read More

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Optimal paracentesis volume for terminally ill cancer patients with ascites.

J Pain Symptom Manage 2021 Apr 29. Epub 2021 Apr 29.

Division of Biostatistics, Tohoku University Graduate School of Medicine, Miyagi, Japan.

Context: Patients with malignant ascites often suffer from distressing symptoms, especially in their end-of-life stage. Although paracentesis is the most common treatment modality to alleviate such symptoms, the optimal volume of paracentesis is not known.

Objectives: To explore the efficacy and safety of paracentesis by the drainage volume for terminally ill cancer patients with malignant ascites. Read More

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Effects of two potential probiotic Lactobacillus bacteria on adipogenesis in vitro.

Life Sci 2021 Apr 28;278:119538. Epub 2021 Apr 28.

School of Biological Sciences, National Institute of Science Education and Research (NISER), P.O. - Bhimpur-Padanpur, Jatni - 752050, Dist.-Khurda, Odisha, India; Homi Bhabha National Institute (HBNI), Training School Complex, Anushaktinagar, Mumbai 400 094, India. Electronic address:

Aims: Overweight is a major global health problem. Various methodologies to get rid of the extra fat are available, but usually, those are associated with adverse side effects. Probiotics, on the contrary, seem to have the potential to help reduce fat accumulation without much apparent adversity. Read More

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Nanopore sequencing reveals TACC2 locus complexity and diversity of isoforms transcribed from an intronic promoter.

Sci Rep 2021 Apr 30;11(1):9355. Epub 2021 Apr 30.

Preventive Medicine and Applied Genomics Unit, RIKEN Center for Integrative Medical Sciences, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.

Gene expression is controlled at the transcriptional and post-transcriptional levels. The TACC2 gene was known to be associated with tumors but the control of its expression is unclear. We have reported that activity of the intronic promoter p10 of TACC2 in primary lesion of endometrial cancer is indicative of lymph node metastasis among a low-risk patient group. Read More

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Paediatric palliative care: a personal perspective.

Acta Paediatr 2021 Apr 30. Epub 2021 Apr 30.

Department of Health Care Sciences, Palliative Research Centre, Ersta Sköndal Bräcke University College, Sweden.

Palliative care as a specialty was developed in the 1960s through the foundation of the hospice movement in United Kingdom aimed at taking care of terminally ill patients with cancer. A definition of palliative care was adopted by WHO in 1990, which, however, is currently being modified to emphasize the importance of lessening suffering. Paediatric palliative care was recognized and defined by WHO in 1998. Read More

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Proper protein folding in the endoplasmic reticulum is required for attachment of a glycosylphosphatidylinositol anchor in plants.

Plant Physiol 2021 Apr 30. Epub 2021 Apr 30.

Department of Applied Genetics and Cell Biology and University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, Austria.

Endoplasmic reticulum (ER) quality control processes recognize and eliminate misfolded proteins to maintain cellular protein homeostasis and prevent the accumulation of defective proteins in the secretory pathway. Glycosylphosphatidylinositol (GPI)-anchored proteins carry a glycolipid modification which provides an efficient ER export signal and potentially prevents the entry into ER-associated degradation (ERAD), which is one of the major pathways for clearance of terminally misfolded proteins from the ER. Here, we analyzed the degradation routes of different misfolded glycoproteins carrying a C-terminal GPI-attachment signal peptide in Arabidopsis thaliana. Read More

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Genomic Spectrum and Phenotypic Heterogeneity of Human IL-21 Receptor Deficiency.

J Clin Immunol 2021 Apr 30. Epub 2021 Apr 30.

Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, NSW, 2010, Australia.

Biallelic inactivating mutations in IL21R causes a combined immunodeficiency that is often complicated by cryptosporidium infections. While eight IL-21R-deficient patients have been reported previously, the natural course, immune characteristics of disease, and response to hematopoietic stem cell transplantation (HSCT) remain to be comprehensively examined. In our study, we have collected clinical histories of 13 patients with IL-21R deficiency from eight families across seven centers worldwide, including five novel patients identified by exome or NGS panel sequencing. Read More

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Heterochromatin and Polycomb as regulators of haematopoiesis.

Biochem Soc Trans 2021 Apr 30. Epub 2021 Apr 30.

The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria3052, Australia.

Haematopoiesis is the process by which multipotent haematopoietic stem cells are transformed into each and every type of terminally differentiated blood cell. Epigenetic silencing is critical for this process by regulating the transcription of cell-cycle genes critical for self-renewal and differentiation, as well as restricting alternative fate genes to allow lineage commitment and appropriate differentiation. There are two distinct forms of transcriptionally repressed chromatin: H3K9me3-marked heterochromatin and H3K27me3/H2AK119ub1-marked Polycomb (often referred to as facultative heterochromatin). Read More

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Development of chlorine-induced lung injury in the anesthetized, spontaneously breathing pig.

Toxicol Mech Methods 2021 May;31(4):257-271

CBR Division, Dstl Porton Down, Salisbury, UK.

Chlorine is a toxic industrial chemical produced in vast quantities globally, being used in a range of applications such as water purification, sanitation and industrial processes. Its use and transport cannot be restricted; exposure may occur following accidental or deliberate releases. The OPCW recently verified the use of chlorine gas against civilians in both Syria and Iraq. Read More

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A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy.

Antib Ther 2020 Dec 9;3(4):227-236. Epub 2020 Nov 9.

Innovent Biologics (Suzhou) Co., Ltd., 168 Dongping Street, Suzhou Industrial Park, Suzhou 215123, Jiangsu, China.

Background: Strategies to reinvigorate exhausted T cells have achieved great efficacy in certain subpopulations of tumor patients. Blocking the antibodies that target programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte-associated protein 4 induces durable responses in Hodgkin's lymphoma, melanoma, renal and lung cancers. T cell immunoglobulin mucin-3 (TIM-3) is another well-defined inhibitory receptor that is expressed in terminally differentiated Th1/Tc1 cells, which produces interferon gamma and cytotoxic molecules. Read More

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December 2020

The Presence of a Marked Imbalance Between Regulatory T Cells and Effector T Cells Reveals That Tolerance Mechanisms Could Be Compromised in Heart Transplant Children.

Transplant Direct 2021 May 23;7(5):e693. Epub 2021 Apr 23.

Laboratory of Immune-regulation, Immunology Department, Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid, Spain.

Regulatory T cells (Treg) are crucial for the induction and maintenance of graft tolerance. In pediatric heart transplant procedures, the thymus is routinely excised, removing the primary source of T-cell replenishment. Consequently, thymectomy joined to the effects of immunosuppression on the T-cell compartment may have a detrimental impact on Treg values, compromising the intrinsic tolerance mechanisms and the protective role of Treg preventing graft rejection in heart transplant children. Read More

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Increased Osteocyte Lacunae Density in the Hypermineralized Bone Matrix of Children with Osteogenesis Imperfecta Type I.

Int J Mol Sci 2021 Apr 26;22(9). Epub 2021 Apr 26.

1st Medical Department, Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, Hanusch Hospital, 1140 Vienna, Austria.

Osteocytes are terminally differentiated osteoblasts embedded within the bone matrix and key orchestrators of bone metabolism. However, they are generally not characterized by conventional bone histomorphometry because of their location and the limited resolution of light microscopy. OI is characterized by disturbed bone homeostasis, matrix abnormalities and elevated bone matrix mineralization density. Read More

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-Related (Neuro)Developmental Disorders: A Question of Causality.

Genes (Basel) 2021 Apr 28;12(5). Epub 2021 Apr 28.

Department of Human Genetics, Donders Institute for Brain, Cognition and Behavior, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.

MED12 is a member of the Mediator complex that is involved in the regulation of transcription. Missense variants in MED12 cause FG syndrome, Lujan-Fryns syndrome, and Ohdo syndrome, as well as non-syndromic intellectual disability (ID) in hemizygous males. Recently, female patients with de novo missense variants and de novo protein truncating variants in MED12 were described, resulting in a clinical spectrum centered around ID and Hardikar syndrome without ID. Read More

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The Role of Advance Care Planning in Cancer Patient and Caregiver Grief Resolution: Helpful or Harmful?

Cancers (Basel) 2021 Apr 20;13(8). Epub 2021 Apr 20.

Cornell Center for Research on End-of-Life Care, Weill Cornell Medicine, New York, NY 10021, USA.

Cancer patients and their family caregivers experience various losses when patients become terminally ill, yet little is known about the grief experienced by patients and caregivers and factors that influence grief as patients approach death. Additionally, few, if any, studies have explored associations between advance care planning (ACP) and grief resolution among cancer patients and caregivers. To fill this knowledge gap, the current study examined changes in grief over time in patients and their family caregivers and whether changes in patient grief are associated with changes in caregiver grief. Read More

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NKL-Code in Normal and Aberrant Hematopoiesis.

Authors:
Stefan Nagel

Cancers (Basel) 2021 Apr 19;13(8). Epub 2021 Apr 19.

Department of Human and Animal Cell Lines, Leibniz-Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, 38124 Braunschweig, Germany.

We have recently described physiological expression patterns of NKL homeobox genes in early hematopoiesis and in subsequent lymphopoiesis and myelopoiesis, including terminally differentiated blood cells. We thereby systematized differential expression patterns of eleven such genes which form the so-called NKL-code. Due to the developmental impact of NKL homeobox genes, these data suggest a key role for their activity in normal hematopoietic differentiation processes. Read More

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CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells.

Genes (Basel) 2021 Apr 8;12(4). Epub 2021 Apr 8.

Institute of Molecular Genetics of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech Republic.

RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dicer isoform, denoted Dicer. Dicer processes dsRNA into small RNAs more efficiently than the full-length Dicer expressed in somatic cells. Read More

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Conformational Plasticity-Rigidity Axis of the Coagulation Factor VII Zymogen Elucidated by Atomistic Simulations of the N-Terminally Truncated Factor VIIa Protease Domain.

Biomolecules 2021 Apr 8;11(4). Epub 2021 Apr 8.

Novo Nordisk Foundation Center for Basic Metabolic Research, Section for Metabolic Receptology, University of Copenhagen, Blegdamsvej 3b, DK-2200 Copenhagen, Denmark.

The vast majority of coagulation factor VII (FVII), a trypsin-like protease, circulates as the inactive zymogen. Activated FVII (FVIIa) is formed upon proteolytic activation of FVII, where it remains in a zymogen-like state and it is fully activated only when bound to tissue factor (TF). The catalytic domains of trypsin-like proteases adopt strikingly similar structures in their fully active forms. Read More

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