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Acute necrotizing encephalopathy-linked mutations in Nup358 impair interaction of Nup358 with TNRC6/GW182 and miRNA function.

Biochem Biophys Res Commun 2021 May 4;559:230-237. Epub 2021 May 4.

National Centre for Cell Science, S.P. Pune University Campus, Pune, 411007, India. Electronic address:

MicroRNA (miRNA)-mediated translational suppression of mRNAs is involved in the regulation of multiple cellular processes. A recent study showed that Nup358, a protein mutated in a neurological disorder called acute necrotizing encephalopathy 1 (ANE1), helps in the coupling of miRNA-induced silencing complex (miRISC) - consisting of miRNA, AGO and GW182/TNRC6 proteins - with the target mRNA. Here we provide a detailed characterization of the interaction between Nup358 and GW182. Read More

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Phylogenetics and historical biogeography of Solanum section Brevantherum (Solanaceae).

Mol Phylogenet Evol 2021 May 4:107195. Epub 2021 May 4.

Programa de Pós-Graduação em Biodiversidade, Universidade Federal do Oeste do Pará, Rua Vera Paz, sn, Santarém, PA, 68040-255, Brazil; Programa de Pós-Graduação em Botânica, Instituto Nacional De Pesquisas Da Amazônia, Av. André Araújo, 2936 - Aleixo, Manaus, AM, 69060-001, Brazil.

Patterns of diversification in Neotropical plants have been studied intensively over the past decades. Most studies have focused on groups that migrated to and radiated into the Neotropics, however, with little focus on understanding diversification patterns in indigenous Neotropical groups. This study focuses on Solanum section Brevantherum Seithe (Solanaceae), a group of Neotropical nightshade shrubs or treelets defined mostly by terminal inflorescences with long peduncles, plurifoliate sympodial units and porrect-stellate, dendritic-echinoid, or lepidote trichomes. Read More

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Spectroscopic and molecular docking study of the interaction between neutral Re(I) tetrazolate complexes and bovine serum albumin.

Chemistry 2021 May 7. Epub 2021 May 7.

University of South Australia, Clinical and Health Sciences, North Terrace, 5000, Adelaide, AUSTRALIA.

Re(I) complexes have potential in biomedical sciences as imaging agents, diagnostics and therapeutics. Thus, it is crucial to understand how Re(I) complexes interact with carrier proteins, like serum albumins. Here, we used two neutral Re(I) complexes ( fac -[Re(CO) 3 (1,10-phenanthroline)L], where L is either 4-cyanophenyltetrazolate (1) or 4-methoxycarbonylphenyltetrazole ester (2) , to study the interactions with bovine serum albumin (BSA). Read More

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Cardiac Troponin T: The Impact of Posttranslational Modifications on Analytical Immunoreactivity in Blood up to the Excretion in Urine.

Adv Exp Med Biol 2021 ;1306:41-59

Unit of Clinical Chemistry, Central Diagnostic Laboratory, Maastricht University Medical Center, Maastricht, The Netherlands.

Cardiac troponin T (cTnT) is a sensitive and specific biomarker for detecting cardiac muscle injury. Its concentration in blood can be significantly elevated outside the normal reference range under several pathophysiological conditions. The classical analytical method in routine clinical analysis to detect cTnT in serum or plasma is a single commercial immunoassay, which is designed to quantify the intact cTnT molecule. Read More

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January 2021

Unique C-terminal extension and interactome of Mycobacterium tuberculosis GlmU impacts its in vivo function and the survival of the pathogen.

Biochem J 2021 May 6. Epub 2021 May 6.

National Institute of Immunology, New Delhi, India.

N-acetyl glucosamine-1-phosphate uridyltransferase (GlmU) is a bifunctional enzyme involved in the biosynthesis of Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc). UDP-GlcNAc is a critical precursor for the synthesis of peptidoglycan and other cell wall components. The absence of a homolog in eukaryotes makes GlmU an attractive target for therapeutic intervention. Read More

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Internalization and membrane activity of the antimicrobial peptide CGA-N12.

Biochem J 2021 May 6. Epub 2021 May 6.

Henan University of Technology, Zhengzhou, China.

Antimicrobial peptides (AMPs) are conventional antibiotic alternatives due to their broad-spectrum antimicrobial activities and special mechanisms of action against pathogens. The antifungal peptide CGA-N12 was originally derived from human chromogranin A (CGA) and consists of the 65th to 76th amino acids of the CGA N-terminal region. In the present study, we found that CGA-N12 had fungicidal activity and exhibited time-dependent inhibition activity against Candida tropicalis. Read More

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The Driver of Extreme Human-Specific Olduvai Repeat Expansion Remains Highly Active in the Human Genome.

Genetics 2020 Jan;214(1):179-191

Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, Colorado 80045.

Olduvai (formerly DUF1220) protein domains have undergone the largest human-specific increase in copy number of any coding region in the genome (∼300 copies of which 165 are human-specific) and have been implicated in human brain evolution... Read More

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January 2020

Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms.

Nat Commun 2021 05 5;12(1):2547. Epub 2021 May 5.

Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.

Receptors and their ligands are important therapeutic targets for about one third of marketed drugs. Here, we describe an epitope-guided approach for selection of antibodies that modulate cellular signaling of targeted receptors. We chose CXC chemokine receptor 2 (CXCR2) in the G-protein coupled receptor superfamily as receptor and a CXCR2 N-terminal peptide for antibody selection. Read More

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The N-terminal 5-68 amino acids domain of the minor capsid protein VP1 of human parvovirus B19 enters human erythroid progenitors and inhibits B19 infection.

J Virol 2021 May 5. Epub 2021 May 5.

Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA

Parvovirus B19 (B19V) infection causes diseases in humans ranging from the mild to severe hematological disorders. The unique region of the minor structural protein VP1 (VP1u) of 227 amino acids harbors strong neutralizing epitopes which elicit dominant immune responses in patients. Recent studies have shown that the VP1u selectively binds to and enters B19V permissive cells through an unknown cellular proteinaceous receptor. Read More

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A new crystal form of GABARAPL2.

Acta Crystallogr F Struct Biol Commun 2021 May 30;77(Pt 5):140-147. Epub 2021 Apr 30.

Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3052, Australia.

The Atg8 protein family comprises the GABA type A receptor-associated proteins (GABARAPs) and microtubule-associated protein 1 light chains 3 (MAP1LC3s) that are essential mediators of autophagy. The LC3-interacting region (LIR) motifs of autophagy receptors and adaptors bind Atg8 proteins to promote autophagosome formation, cargo recruitment, and autophagosome closure and fusion to lysosomes. A crystal structure of human GABARAPL2 has been published [PDB entry 4co7; Ma et al. Read More

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DOCK8-related Immunodeficiency Syndrome (DIDS): Report of Novel Mutations in Iranian Patients.

J Mol Neurosci 2021 May 4. Epub 2021 May 4.

Division of Medical Genetics, Tabriz Children's Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.

DOCK8 immunodeficiency syndrome (DIDS) is a rare autosomal recessive (AR) disorder characterized by elevated serum IgE levels, eosinophilia, recurrent cutaneous infections, severe eczema, and sinopulmonary and gastrointestinal infections. This syndrome is a multisystem disease that is associated with both immune deficiency and neurological complications. In this study, we describe the clinical characteristics of two Iranian patients with DOCK8 deficiency and propose possible mechanisms for this condition. Read More

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Attenuation of inhibitory PAS domain protein-induced cell death by synthetic peptides derived from Mcl-1 transmenbrane domain.

Cell Death Discov 2021 May 4;7(1):92. Epub 2021 May 4.

Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Aoba-ku Sendai, 980-8578, Japan.

Expression of Inhibitory PAS domain protein (IPAS) induces apoptosis by inhibiting the anti-apoptotic activity of mitochondrial pro-survival proteins including Bcl-x and Mcl-1 through direct binding. Analysis to examine the IPAS-binding region in Bcl-x demonstrated that the C-terminal transmembrane (TM) domain is indispensable for the specific binding. A chimeric protein composed of the TM domain of Mcl-1 fused to the C-terminus of Citrine also exhibited a binding affinity to IPAS, and markedly attenuated apoptosis caused by the overexpression of Cerulean-IPAS in SH-SY5Y cells. Read More

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Site-Specific Conversion of Cysteine in a Protein to Dehydroalanine Using 2-Nitro-5-thiocyanatobenzoic Acid.

Molecules 2021 Apr 29;26(9). Epub 2021 Apr 29.

The Texas A&M Drug Discovery Laboratory, Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.

Dehydroalanine exists natively in certain proteins and can also be chemically made from the protein cysteine. As a strong Michael acceptor, dehydroalanine in proteins has been explored to undergo reactions with different thiolate reagents for making close analogues of post-translational modifications (PTMs), including a variety of lysine PTMs. The chemical reagent 2-nitro-5-thiocyanatobenzoic acid (NTCB) selectively modifies cysteine to form -cyano-cysteine, in which the S-Cβ bond is highly polarized. Read More

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Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis.

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

Institute of Systems Biology, Pusan National University, Busan 46241, Korea.

Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, the functions and molecular aspects of many non-tandem CCCH zinc finger (non-TZF) proteins remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein and belongs to the ZFWD subfamily harboring a CCCH zinc finger motif and a WD40 domain. In this study, we characterized the biological and molecular functions of AtC3H59, which is subcellularly localized in the nucleus. Read More

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Trimerization of the N-Terminal Tail of Zika Virus NS4A Protein: A Potential In Vitro Antiviral Screening Assay.

Membranes (Basel) 2021 Apr 30;11(5). Epub 2021 Apr 30.

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.

The nonstructural (NS) protein NS4A in flaviviruses is a membrane protein that is critical for virulence, and, among other roles, it participates in membrane morphogenesis. In dengue virus (DENV), the NS4A hydrophilic N-terminal tail, together with the first transmembrane domain, is involved in both homo-oligomerization and hetero-oligomerization with NS4B. In both DENV and Zika virus (ZIKV), this N-terminal tail (residues 1-48) forms a random coil in solution but becomes mostly α-helical upon interaction with detergents or lipid membranes. Read More

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D936Y and Other Mutations in the Fusion Core of the SARS-CoV-2 Spike Protein Heptad Repeat 1: Frequency, Geographical Distribution, and Structural Effect.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

Kaust Catalysis Center, Physical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

The crown of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is constituted by its spike (S) glycoprotein. S protein mediates the SARS-CoV-2 entry into the host cells. The "fusion core" of the heptad repeat 1 (HR1) on S plays a crucial role in the virus infectivity, as it is part of a key membrane fusion architecture. Read More

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The nuclear localization sequence of the epigenetic factor RYBP binds to human importin α3.

Biochim Biophys Acta Proteins Proteom 2021 May 1;1869(8):140670. Epub 2021 May 1.

Institute for Biocomputation and Physics of Complex Systems (BIFI), Joint Units IQFR-CSIC-BIFI, GBsC-CSIC-BIFI, Universidad de Zaragoza, 50018 Zaragoza, Spain; Instituto de Investigación Sanitaria Aragón (IIS Aragón), 50009 Zaragoza, Spain; Fundación ARAID, Government of Aragón, 50018 Zaragoza, Spain; Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, 50009 Zaragoza, Spain; Centro de Investigación Biomédica en Red en el Área Temática de Enfermedades Hepáticas y Digestivas (CIBERehd), 28006 Madrid, Spain.

RYBP (Ring1 and YY1 binding protein, UniProt ID: Q8N488) is an epigenetic factor with a key role during embryonic development; it does also show an apoptotic function and an ubiquitin binding activity. RYBP is an intrinsically disordered protein (IDP), with a Zn-finger domain at its N-terminal region, which folds upon binding to DNA. It is predicted that RYBP has a nuclear localization sequence (NLS), comprising residues Asn58 to Lys83, to allow for nuclear translocation. Read More

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The translation initiation factor EIF4E5 from Leishmania: crystal structure and interacting partners.

RNA Biol 2021 May 4:1-17. Epub 2021 May 4.

Departamento de Microbiologia, Instituto Aggeu Magalhães, FIOCRUZ-PE, Av. Moraes Rego s/n, Recife-PE, Brazil.

The mRNA cap-binding protein, eIF4E, mediates the recognition of the mRNA 5' end and, as part of the heterotrimeric eIF4F complex, facilitates the recruitment of the ribosomal subunits to initiate eukaryotic translation. Various regulatory events involving eIF4E and a second eIF4F subunit, eIF4G, are required for proper control of translation initiation. In pathogenic trypanosomatids, six eIF4Es and five eIF4Gs have been described, several forming different eIF4F-like complexes with yet unresolved roles. Read More

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Sec17/Sec18 can support membrane fusion without help from completion of SNARE zippering.

Elife 2021 May 4;10. Epub 2021 May 4.

Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States.

Membrane fusion requires R-, Qa-, Qb-, and Qc-family SNAREs that zipper into RQaQbQc coiled coils, driven by the sequestration of apolar amino acids. Zippering has been thought to provide all the force driving fusion. Sec17/aSNAP can form an oligomeric assembly with SNAREs with the Sec17 C-terminus bound to Sec18/NSF, the central region bound to SNAREs, and a crucial apolar loop near the N-terminus poised to insert into membranes. Read More

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Analysis of Combined Indicators for Risk of Osteoporotic Hip Fracture in Elderly Women.

Orthop Surg 2021 May 4. Epub 2021 May 4.

Department of Orthopaedic Surgery, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China.

Objective: To compare the accuracy of combined independent risk factors in assessing the risk of hip fractures in elderly women.

Methods: Ninety elderly females who sustained hip fractures (including femoral neck fractures and intertrochanteric fractures) and 110 female outpatients without a hip fracture were included in our cross-sectional study from 24 November 2017 to 20 May 2019. The age of subjects in the present study was ≥65 years, with the mean age of 78. Read More

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Robust α-synuclein pathology in select brainstem neuronal populations is a potential instigator of multiple system atrophy.

Acta Neuropathol Commun 2021 May 3;9(1):80. Epub 2021 May 3.

Department of Neuroscience, College of Medicine, University of Florida, BMS J483/CTRND, 1275 Center Drive, Gainesville, FL, 32610, USA.

Multiple system atrophy (MSA) is an insidious middle age-onset neurodegenerative disease that clinically presents with variable degrees of parkinsonism and cerebellar ataxia. The pathological hallmark of MSA is the progressive accumulation of glial cytoplasmic inclusions (GCIs) in oligodendrocytes that are comprised of α-synuclein (αSyn) aberrantly polymerized into fibrils. Experimentally, MSA brain samples display a high level of seeding activity to induce further αSyn aggregation by a prion-like conformational mechanism. Read More

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D-Rhamnan and Pyruvate-Containing Teichuronic Acid from the Cell Wall of Rathayibacter sp. VKM Ac-2759.

Biochemistry (Mosc) 2021 Apr;86(4):506-516

All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.

Rathayibacter sp. VKM Ac-2759 (family Microbacteriaceae, class Actinobacteria) contains two glycopolymers in the cell wall. The main chain of rhamnan, glycopolymer 1, is built from the repeating tetrasaccharide units carrying terminal arabinofuranose residues at the non-reducing end, →3)-α-[α-D-Araf-(1→2)]-D-Rhap-(1→2)-α-D-Rhap-(1→3)-α-D-Rhap-(1→2)-α-D-Rhap-(1→. Read More

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DNA-binding domain as the minimal region driving RNA-dependent liquid-liquid phase separation of androgen receptor.

Protein Sci 2021 May 2. Epub 2021 May 2.

VIB-VUB Center for Structural Biology, Vlaams Instituut voor Biotechnology, Brussels, Belgium.

Androgen receptor (AR) is a nuclear hormone receptor that regulates the transcription of genes involved in the development of testis, prostate and the nervous system. Misregulation of AR is a major driver of prostate cancer (PC). The primary agonist of full-length AR is testosterone, whereas its splice variants, for example, AR-v7 implicated in cancer may lack a ligand-binding domain and are thus devoid of proper hormonal control. Read More

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Structure and Membrane Targeting of the PDZD7 Harmonin Homology Domain (HHD) Associated With Hearing Loss.

Front Cell Dev Biol 2021 15;9:642666. Epub 2021 Apr 15.

Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.

Usher syndrome (USH) is the leading cause of hereditary hearing-vision loss in humans. PDZ domain-containing 7 (PDZD7) has been reported to be a modifier of and contributor to USH. PDZD7 co-localizes with USH2 proteins in the inner ear hair cells and is essential for ankle-link formation and stereocilia development. Read More

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Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein.

Front Cell Infect Microbiol 2021 14;11:656620. Epub 2021 Apr 14.

Division of Medical Zoology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, Japan.

Erythrocyte recognition and invasion is critical for the intra-erythrocytic development of spp. parasites. The multistep invasion process involves specific interactions between parasite ligands and erythrocyte receptors. Read More

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Genome wide identification, phylogeny, and synteny analysis of family in common carp ().

Biotechnol Rep (Amst) 2021 Jun 16;30:e00607. Epub 2021 Mar 16.

Division of Fish Genetics and Biotechnology, Fauclty of Fisheries Rangil, Ganderbal, SKUAST-Kashmir, India.

Common carp () is a commercial fish species valuable for nutritious components and plays a vital role in human healthy nutrition. The SOX (SRY-related genes systematically characterized by a high-mobility group HMG-box) encoded important gene regulatory proteins, a family of transcription factors found in a broad range of animal taxa and extensively known for its contribution in multiple developmental processes including contribution in sex determination across phyla. In our current study, we initially accomplished a genome-wide analysis to report the SOX gene family in common carp fish based on available genomic sequences of zebrafish retrieved from gene repository databases, we focused on the global identification of the Sox gene family in Common carp among wide range of vertebrates and teleosts based on bioinformatics tools and techniques and explore the evolutionary relationships. Read More

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Molecular analysis of goose parvovirus field strains from a Derzsy's disease outbreak reveals local European-associated variants.

Arch Virol 2021 May 2. Epub 2021 May 2.

Department of Internal Medicine, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Turkey.

Since its first recognition in the early 1960s, Derzsy's disease has caused significant economic losses in the goose meat industry through the world. Today, Derzsy's disease still maintains its importance for small-scale waterfowl farming, despite not having a significant impact on public health. In the present study, we investigated the distribution of goose parvovirus (GPV) and its potential variants from a 2019 outbreak in Turkey. Read More

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HLA class II presentation is specifically altered at elevated temperatures in the B-lymphoblastic cell line JY.

Mol Cell Proteomics 2021 Apr 29:100089. Epub 2021 Apr 29.

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, the Netherlands. Electronic address:

HLA class I and HLA class II molecules play critical roles in our adaptive immune system by signaling a cell's health status to the immune system, through presentation of small peptides. Understanding HLA biology is important due to its prominent implication in auto-immune diseases and role in cancer immuno-therapy. Although both the HLA class I and class II antigen processing and presentation pathways have been studied extensively, the fundamental rules in HLA class II antigen presentation still remain less understood. Read More

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The differential solvent exposure of N-terminal residues provides 'fingerprints' of alpha-synuclein fibrillar polymorphs.

J Biol Chem 2021 Apr 29:100737. Epub 2021 Apr 29.

Laboratory of Neurodegenerative Diseases, Institut Francois Jacob (MIRCen), CNRS, CEA, University Paris-Saclay, 92265 Fontenay-Aux-Roses cedex, France.

Synucleinopathies are neurodegenerative diseases characterized by the presence of intracellular deposits containing the protein alpha-synuclein (aSYN) within patients' brains. It has been shown that aSYN can form structurally distinct fibrillar assemblies, also termed polymorphs. We previously showed that distinct aSYN polymorphs assembled in vitro, named fibrils, ribbons and fibrils 91, differentially bind to and seed the aggregation of endogenous aSYN in neuronal cells, which suggests that distinct synucleinopathies may arise from aSYN polymorphs. Read More

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The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form.

J Biol Chem 2021 Apr 29:100731. Epub 2021 Apr 29.

School of Biotechnology and Biomolecular Sciences, UNSW Sydney, NSW 2052, Australia. Electronic address:

Squalene monooxygenase (SM, also known as squalene epoxidase) is a rate-limiting enzyme of cholesterol synthesis that converts squalene to monooxidosqualene and is oncogenic in numerous cancer types. SM is subject to feedback regulation via cholesterol-induced proteasomal degradation, which depends on its lipid-sensing N-terminal regulatory domain. We previously identified an endogenous truncated form of SM with a similar abundance to full-length SM, but whether this truncated form is functional or subject to the same regulatory mechanisms as full-length SM is not known. Read More

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