1,126,417 results match your criteria technique


Demystifying the spontaneous phenomena of motor hyperexcitability.

Clin Neurophysiol 2021 May 13;132(8):1830-1844. Epub 2021 May 13.

UK Dementia Research Institute, Department of Basic and Clinical Neuroscience, Maurice Wohl Clinical Neuroscience Institute, Institute of Psychiatry, Psychology and Neuroscience, King's College London, UK; King's College Hospital NHS Foundation Trust, Denmark Hill, London, UK.

Possessing a discrete functional repertoire, the anterior horn cell can be in one of two electrophysiological states: on or off. Usually under tight regulatory control by the central nervous system, a hierarchical network of these specialist neurons ensures muscular strength is coordinated, gradated and adaptable. However, spontaneous activation of these cells and their axons can result in abnormal muscular twitching. Read More

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Fullerenol as a water-soluble MALDI-MS matrix for rapid analysis of small molecules and efficient quantification of saccharin sodium in foods.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jun 4;1178:122819. Epub 2021 Jun 4.

Henan Key Laboratory of Organic Functional Molecule and Drug Innovation, Key Laboratory of Green Chemical Media and Reactions of Ministry of Education, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, China. Electronic address:

Due to the strong background interferences in the low-mass region and poor reproducibility of conventional organic matrices, it is of great importance to develop a novel matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to qualitatively and quantitatively analyze small molecules. In this work, water-soluble fullerenol C(OH) was selected as a MALDI matrix for the analysis of low-molecular-weight compounds in consideration of optical absorption property, water solubility and stability. Compared with the traditional matrices, fullerenol demonstrated lower background interference and stronger peak intensity. Read More

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Microplastic degradation methods and corresponding degradation mechanism: Research status and future perspectives.

J Hazard Mater 2021 Jun 10;418:126377. Epub 2021 Jun 10.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Institute of Eco-Environmental Research, Guangxi Academy of Sciences, Nanning 530007, China. Electronic address:

Microplastics (MPs) pollution has become a global environmental concern because of their severe threat to biota. However, limited studies on the elimination of MPs pollution were reported. The conventional treatment methods were not suitable for MPs owing to their smaller size than plastic items. Read More

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A biomechanical model for testing cage subsidence in spine specimens with osteopenia or osteoporosis under permanent maximum load.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

Department of Orthopedic, Trauma and Plastic Surgery, University Hospital Leipzig. Electronic address:

Intervertebral fusions in cases of reduced bone density are a tough challenge. From a biomechanical point of view, the majority of current studies have focused on the range of motion or have shown test setups for single component tests. Definite setups for biomechanical testing of the primary stability of a 360° fusion using a screw rod system and cage on the osteoporotic spine are missing. Read More

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Optic nerve mobilization as an alternative to anterior clinoidectomy in superior carotid-ophthalmic aneurysms: Operative technique.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

Department of Neurosurgery, Padilla Hospital, Tucumán, Argentina.

Background: Carotid-ophthalmic aneurysms arise from the internal carotid artery between the distal dural ring and the origin of the posterior communicating artery. The surgical treatment of these aneurysms usually requires an anterior clinoidectomy. However, this procedure is not without complications. Read More

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Evaluation on effectiveness of treatment strategies for degenerative lumbar spinal stenosis: a systematic review and network meta-analysis of clinical studies.

World Neurosurg 2021 Jun 12. Epub 2021 Jun 12.

The Center for Pain Medicine, Peking University Third Hospital, Beijing 100191, China. Electronic address:

Objective: Lumbar spinal stenosis (LSS) is a common disease in spinal surgery. At present, many related treatment methods have been reported, but their effectiveness still lacks a systematic comparison. We aimed to evaluate the clinical outcomes related to the efficacy and safety of these treatment strategies via a network meta-analysis. Read More

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Sensitive and quantitative detection of MHC-I displayed neoepitopes using a semi-automated workflow and TOMAHAQ mass spectrometry.

Mol Cell Proteomics 2021 Jun 12:100108. Epub 2021 Jun 12.

Genentech, South San Francisco, 94080, California, USA. Electronic address:

Advances in several key technologies, including MHC peptidomics, have helped fuel our understanding of basic immune regulatory mechanisms and the identification of T cell receptor targets for the development of immunotherapeutics. Isolating and accurately quantifying MHC-bound peptides from cells and tissues enables characterization of dynamic changes in the ligandome due to cellular perturbations. However, the current multi-step analytical process is challenging, and improvements in throughput and reproducibility would enable rapid characterization of multiple conditions in parallel. Read More

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Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia.

Parasitol Int 2021 Jun 12:102404. Epub 2021 Jun 12.

Institute of Veterinary Medicine, Mongolian University of Life Science, Zaisan, 17042, Ulaanbaatar, Mongolia. Electronic address:

Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. Read More

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Interleukin 4 and Interleukin 4 receptor alpha gene variants and risk of atopy - A case control study based assessment.

Clin Immunol 2021 Jun 12:108783. Epub 2021 Jun 12.

Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences (SKIMS), Soura, 190011, J&K, India. Electronic address:

Introduction: IL4 pathway is known to upregulate IgE mediated immune responses and responsible for the manifestation of Atopic disorders. The current study was aimed to elucidate the genetic variations of Interleukin 4 (IL4) and Interleukin 4 receptor alpha (IL4R) genes and their possible association with atopic subjects.

Methods: The well-designed questionnaire was used to collect the subject demographic and clinical details. Read More

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Endoscopic Septotomy Of Zenker's Diverticulum With Stag-Beetle Knife: A Descriptive Observational Study And Literature Review.

Gastroenterol Hepatol 2021 Jun 12. Epub 2021 Jun 12.

UGC Aparato Digestivo Hospital Universitario Virgen de la Victoria, Málaga, Spain.

Introduction: Zenker's diverticulum (ZD) is a protrusion of the hypopharyngeal mucosa with a prevalence of 2 / 100,000 inhabitants. The symptoms of the patients determine the need for treatment, which can be surgical or endoscopic. The latter, known as endoscopic septotomy or diverticulotomy (ED), this involves dissecting the diverticular septum, which can be performed with different dissection devices. Read More

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The funny current in genetically modified mice.

Prog Biophys Mol Biol 2021 Jun 12. Epub 2021 Jun 12.

Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France; LabEx Ion Channels Science and Therapeutics (ICST), France. Electronic address:

Since its first description in 1979, the hyperpolarization-activated funny current (I) has been the object of intensive research aimed at understanding its role in cardiac pacemaker activity and its modulation by the sympathetic and parasympathetic branches of the autonomic nervous system. I was described in isolated tissue strips of the rabbit sinoatrial node using the double-electrode voltage-clamp technique. Since then, the rabbit has been the principal animal model for studying pacemaker activity and I for more than 20 years. Read More

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Intra operative assessment of the coronal balance in spinal deformity surgery : a technical note and retrospective study.

Acta Orthop Belg 2021 Mar;87(1):175-179

Obtaining a spine that is well balanced after fusion for scoliotic deformity is primordial for the patients' quality of life. A simple T-shaped instrument combined with standard intraoperative fluoroscopy can be of great help to evaluate the coronal alignment quickly. The aim of this study was to evaluate if a T-shaped device could predict the postoperative coronal balance. Read More

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Proximal femoral derotation osteotomy in children with CP : long term outcome and the role of age at time of surgery.

Acta Orthop Belg 2021 Mar;87(1):167-173

The femoral derotation osteotomy (FDO) is seen as the golden standard treatment in children with cerebral palsy and internal rotated gait. This study provides quantitative evidence in support of the beneficial effect of FDO after long term follow up. Retrospective clinical and kinematic evaluation of 31 CP patients (55 operated limbs) pre-, 1 and 3 years postoperatively after proximal FDO was conducted for a minimal follow-up of 3 years. Read More

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Functional and radiological outcome after forearm plating in children and adolescent fracture.

Acta Orthop Belg 2021 Mar;87(1):143-149

The literature on forearm overgrowth after plating in traumatic conditions is relatively poor though this technique can be useful when intra-medullary nailing is not sufficient in pediatric cases. The goal of this study was to assess a potential overgrowth after plating and identify impact on function. We conducted a retrospective study of all pediatric patients who underwent open surgery of the radius and/or ulna diaphysis with internal fixation by plating, in our institution, between October 2013 and July 2019. Read More

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Subcuticular sutures versus staples for skin closure after primary hip arthroplasty.

Acta Orthop Belg 2021 Mar;87(1):55-64

High-quality and cost-effective health care are highly recommended especially in joint replacement surgeries, particularly in total hip arthroplasty. Therefore, it is indispensable for orthopaedic surgeons to spot the potential areas of quality improvement. Evaluating the efficacy of the different ways of skin closure is an unacknowledged topic. Read More

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Novel troubleshooting technique for the migration of a biliary plastic stent using a thin balloon catheter (with video).

J Hepatobiliary Pancreat Sci 2021 Jun 15. Epub 2021 Jun 15.

Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, Osaka-Sayama, Japan.

Plastic stents (PSs) are commonly used to for obstructive jaundice (1, 2), but there are difficulties associated with removing migrated PSs. (3-5) A 75-year-old woman presented with obstructive jaundice due to hilar cholangiocarcinoma, and two PSs were inserted. After two years, re-intervention for the occlusion of PSs was performed. Read More

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Unraveling the Interplay of Image Formation, Data Representation and Learning in CT-based COPD Phenotyping Automation: The Need for a Meta-Strategy.

Med Phys 2021 Jun 15. Epub 2021 Jun 15.

Siemens Healthineers, CT R&D Image Analytics, Forchheim, Germany.

Purpose: In the literature on automated phenotyping of Chronic Obstructive Pulmonary Disease (COPD), there is a multitude of isolated classical machine learning and deep learning techniques, mostly investigating individual phenotypes, with small study cohorts and heterogeneous meta-parameters, e.g. different scan protocols or segmented regions. Read More

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Comparison of foot strike sound between rearfoot, midfoot and forefoot strike runners.

J Athl Train 2021 Jun 15. Epub 2021 Jun 15.

Gait & Motion Analysis Laboratory, Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hong Kong.

Context: There are three common foot strike techniques in runners. Whether these techniques generate different sounds at the point of impact with the ground may influence lower limb kinetics. No previous studies have determined whether such relationships exist. Read More

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The selection of indicators from initial blood routine test results to improve the accuracy of early prediction of COVID-19 severity.

PLoS One 2021 15;16(6):e0253329. Epub 2021 Jun 15.

The Geriatric Respiratory Department, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

The global pandemic of COVID-19 poses a huge threat to the health and lives of people all over the world, and brings unprecedented pressure to the medical system. We need to establish a practical method to improve the efficiency of treatment and optimize the allocation of medical resources. Due to the influx of a large number of patients into the hospital and the running of medical resources, blood routine test became the only possible check while COVID-19 patients first go to a fever clinic in a community hospital. Read More

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Value of spectral detector computed tomography for the early assessment of technique efficacy after microwave ablation of hepatocellular carcinoma.

PLoS One 2021 15;16(6):e0252678. Epub 2021 Jun 15.

Faculty of Medicine and University Hospital Cologne, Department of Diagnostic and Interventional Radiology, University of Cologne, Cologne, Germany.

Objectives: To investigate whether virtual monoenergetic images (VMI) and iodine maps derived from spectral detector computed tomography (SDCT) improve early assessment of technique efficacy in patients who underwent microwave ablation (MWA) for hepatocellular carcinoma (HCC) in liver cirrhosis.

Methods: This retrospective study comprised 39 patients with 49 HCC lesions treated with MWA. Biphasic SDCT was performed 7. Read More

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Serial imaging of micro-agents and cancer cell spheroids in a microfluidic channel using multicolor fluorescence microscopy.

PLoS One 2021 15;16(6):e0253222. Epub 2021 Jun 15.

Surgical Robotics Laboratory, Department of Biomechanical Engineering, University of Twente, Enschede, The Netherlands.

Multicolor fluorescence microscopy is a powerful technique to fully visualize many biological phenomena by acquiring images from different spectrum channels. This study expands the scope of multicolor fluorescence microscopy by serial imaging of polystyrene micro-beads as surrogates for drug carriers, cancer spheroids formed using HeLa cells, and microfluidic channels. Three fluorophores with different spectral characteristics are utilized to perform multicolor microscopy. Read More

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Laboratory evaluation of the rapid diagnostic tests for the detection of Vibrio cholerae O1 using diarrheal samples.

PLoS Negl Trop Dis 2021 Jun 15;15(6):e0009521. Epub 2021 Jun 15.

Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

Background: Cholera, an acute diarrheal disease is a major public health problem in many developing countries. Several rapid diagnostic tests (RDT) are available for the detection of cholera, but their efficacies are not compared in an endemic setting. In this study, we have compared the specificity and sensitivity of three RDT kits for the detection of Vibrio cholerae O1 and compared their efficiency with culture and polymerase chain reaction (PCR) methods. Read More

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Two-dimensional vector accelerometer based on orthogonal Bragg gratings inscribed in a standard single-mode fiber cladding.

Opt Lett 2021 Jun;46(12):2992-2995

We propose and demonstrate a novel, to the best of our knowledge, two-dimensional vector accelerometer based on orthogonal cladding fiber Bragg gratings (FBGs) inscribed in a standard single-mode fiber (SMF). The cladding FBGs are written by a femtosecond laser point-by-point technique and run parallel with the core. We experimentally demonstrate that the two orthogonal components of acceleration can be directly detected using simplified power-referenced detection. Read More

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In-service crosstalk monitoring in multicore fibers based on precoded DMT system.

Opt Lett 2021 Jun;46(12):2924-2927

In-service crosstalk monitoring based on the precoding technique in a discrete multitone (DMT) system is proposed and validated experimentally. The method relies on the ability of time-frequency domain equalization of precoded DMT. Experiments on a 20 GBaud 16 quadrature amplitude modulation DMT system over seven-core weakly coupled multicore fibers (MCFs) are conducted. Read More

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Common-path angular-multiplexing holographic data storage based on computer-generated holography.

Opt Lett 2021 Jun;46(12):2920-2923

An unconventional angular-multiplexed recording technique is proposed for holographic data storage based on a computer-generated hologram (CGH) technique. While general angular-multiplexed recording techniques require a Mach-Zehnder interferometer to record data pages as volume holograms, the proposed method records ones with a common-path configuration with the help of a CGH technique, which prevents the optical setup from being bulky. In the proposed method, the CGH reconstructs signal and reference beams simultaneously, and these beams interfere in a recording medium. Read More

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Phase-shifted demodulation technique with additional modulation based on a 3 × 3 coupler and EFA for the interrogation of fiber-optic interferometric sensors.

Opt Lett 2021 Jun;46(12):2900-2903

A phase-shifted demodulation technique with a 3×3 coupler and ellipse fitting algorithm (EFA) for the interrogation of interferometric sensors is proposed. To reduce the error of the EFA as to measure small phase signals, additional phase modulation is introduced. The additional modulation provides a walk of the operating point along the Lissajous ellipse large enough to permit calculation of the ellipse parameters at every moment. Read More

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Large refractive index modulation based on a BDK-doped step-index PMMA optical fiber for highly reflective Bragg grating inscription.

Opt Lett 2021 Jun;46(12):2864-2867

We experimentally report high reflectivity on the poly(methyl methacrylate) (PMMA)-based polymer optical fiber Bragg gratings by means of a 266 nm pulsed laser and phase mask technique. In the first recipe, fiber Bragg gratings (FBGs) were manufactured with a single pulse up to 3.7 mJ. Read More

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Resource-effective quantum key distribution: a field trial in Padua city center.

Opt Lett 2021 Jun;46(12):2848-2851

Field trials are of key importance for novel technologies seeking commercialization and widespread adoption. This is also the case for quantum key distribution (QKD), which allows distant parties to distill a secret key with unconditional security. Typically, QKD demonstrations over urban infrastructures require complex stabilization and synchronization systems to maintain a low quantum bit error and high secret key rates over time. Read More

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Method to obtain the initial value for the inverse design in nanophotonics based on a time-reversal technique.

Opt Lett 2021 Jun;46(12):2815-2818

In the inverse design of nanophotonic devices, mathematical optimization methods are generally used to perform local optimization in the design region to obtain the physical structure that meets design expectations. These methods usually produce good structures. However, due to the lack of physical considerations, most of the inverse design methods for nanophotonic devices use random initial topology as the initialization for optimization, which will inevitably cause a waste of computing resources. Read More

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Full-field measurement of complex objects illuminated by an ultrashort pulse laser using delay-line sweeping off-axis interferometry.

Opt Lett 2021 Jun;46(12):2803-2806

Measuring the complex field of ultrashort pulse lasers plays a fundamental role in light wavefront manipulation and nonlinear phenomena investigation; yet, it still constitutes a challenge for both full-field and high-resolution characterization due to the short coherent length. We proposed and implemented an off-axis interference system with a delay-line sweeping technique to overcome the fringe contrast degradation caused by the envelope mismatch between interfering pulses, resulting in an increased effective analysis area. The effectiveness of the proposed method was first demonstrated by measuring a complex field generated by a phase-only spatial light modulator, where a four-pixel binning technique was adopted for both amplitude and phase modulation; then it was used for the measurement of the second harmonic generation signal of a urea crystal sample. Read More

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