6,339 results match your criteria technical details

Synovial chondromatosis of the temporomandibular joint. Technical details of arthroscopic management.

Br J Oral Maxillofac Surg 2020 Dec 17. Epub 2020 Dec 17.

Hospital Universitario Virgen de las Nieves, Granada, Spain.

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December 2020

Reconstruction of C1 lateral mass with an expandable cage in addition to vertebral artery preservation: presenting two cases.

Br J Neurosurg 2021 Sep 17:1-6. Epub 2021 Sep 17.

Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.

Purpose: C1 lateral mass reconstruction is recommended, in cases of instability caused by tumor involvement or extensive C1 lateral mass resection. However, because of the anatomical complexity of the area and, most importantly, the proximity to vertebral arteries, few cases of reconstruction have been reported to date. The purpose of this report is to present technical details of C1 lateral mass reconstruction in conjunction with vertebral artery preservation from a posterior approach. Read More

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September 2021

Purification of GFP-tagged nuclei from frozen livers of INTACT mice for RNA- and ATAC-sequencing.

STAR Protoc 2021 Sep 8;2(3):100805. Epub 2021 Sep 8.

Institute for Diabetes and Cancer (IDC), Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg 85764, Germany.

Isolation of nuclei tagged in specific cell types (INTACT) allows for stress-free and high-throughput analyses of cellular subpopulations. Here, we present an improved protocol for isolation of pure and high-quality GFP-labeled nuclei from frozen livers of INTACT mice, as well as protocols for downstream sequencing analyses. The adaptation to frozen tissue provides a pause point that allows sampling at multiple time points and/or phenotypic characterization of livers prior to nuclei isolation and downstream analyses. Read More

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September 2021

High-resolution analysis of the conformational transition of pro-apoptotic Bak at the lipid membrane.

EMBO J 2021 Sep 15:e107159. Epub 2021 Sep 15.

Bavarian NMR Center at the Department of Chemistry, Technical University of Munich, Garching, Germany.

Permeabilization of the outer mitochondrial membrane by pore-forming Bcl2 proteins is a crucial step for the induction of apoptosis. Despite a large set of data suggesting global conformational changes within pro-apoptotic Bak during pore formation, high-resolution structural details in a membrane environment remain sparse. Here, we used NMR and HDX-MS (Hydrogen deuterium exchange mass spectrometry) in lipid nanodiscs to gain important high-resolution structural insights into the conformational changes of Bak at the membrane that are dependent on a direct activation by BH3-only proteins. Read More

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September 2021

Teaching Antenatal Counseling Skills via Video Conference.

Cureus 2021 Aug 9;13(8):e17030. Epub 2021 Aug 9.

Neonatology, Seattle Children's Hospital/University of Washington School of Medicine, Seattle, USA.

Neonatologists provide counseling to expectant parents to prepare them for the birth and subsequent medical care that their extremely preterm, or otherwise medically complex newborn may require. The skills required to conduct these sensitive conversations are often taught to neonatology trainees via direct observation or simulated scenarios in advance of counseling actual patients. This technical report details how we taught antenatal counseling skills to junior neonatal-perinatal medicine (NPM) fellows via video conferencing during the coronavirus disease 2019 (COVID-19) pandemic. Read More

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Guidelines for reporting methods to estimate metabolic rates by aquatic intermittent-flow respirometry.

J Exp Biol 2021 Sep 14;224(18). Epub 2021 Sep 14.

MARBEC, Université Montpellier, CNRS, Ifremer, IRD, 34000 Montpellier, France.

Interest in the measurement of metabolic rates is growing rapidly, because of the importance of metabolism in advancing our understanding of organismal physiology, behaviour, evolution and responses to environmental change. The study of metabolism in aquatic animals is undergoing an especially pronounced expansion, with more researchers utilising intermittent-flow respirometry as a research tool than ever before. Aquatic respirometry measures the rate of oxygen uptake as a proxy for metabolic rate, and the intermittent-flow technique has numerous strengths for use with aquatic animals, allowing metabolic rate to be repeatedly estimated on individual animals over several hours or days and during exposure to various conditions or stimuli. Read More

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September 2021

SARS-CoV-2 structural coverage map reveals viral protein assembly, mimicry, and hijacking mechanisms.

Mol Syst Biol 2021 09;17(9):e10079

Department of Informatics, Bioinformatics & Computational Biology, Technical University of Munich, Munich, Germany.

We modeled 3D structures of all SARS-CoV-2 proteins, generating 2,060 models that span 69% of the viral proteome and provide details not available elsewhere. We found that ˜6% of the proteome mimicked human proteins, while ˜7% was implicated in hijacking mechanisms that reverse post-translational modifications, block host translation, and disable host defenses; a further ˜29% self-assembled into heteromeric states that provided insight into how the viral replication and translation complex forms. To make these 3D models more accessible, we devised a structural coverage map, a novel visualization method to show what is-and is not-known about the 3D structure of the viral proteome. Read More

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September 2021

Flexible Ureterorenoscopy under Spinal Anesthesia: Focus on Technique, Results, Complications, and Patients' Satisfaction from a Large Series.

Urol Int 2021 Sep 8:1-6. Epub 2021 Sep 8.

Department of Urology, Città della Salute e della Scienza, Molinette University Hospital, Turin, Italy.

Introduction: Although spinal anesthesia (SA) may reduce postoperative morbidity, most urologists perform flexible ureterorenoscopy (fURS) under general anesthesia (GA). The objective of our study is to provide technical details, results, complications, and patients' satisfaction with fURS performed under exclusive SA.

Methods: We analyzed all consecutive fURS performed under SA to treat renal stones from March 2011 to February 2017. Read More

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September 2021

Robotic Intracorporeal Anastomosis.

Clin Colon Rectal Surg 2021 Sep 8;34(5):334-337. Epub 2021 Sep 8.

Division of Colon and Rectal Surgery, Department of Surgery, Roper Hospital, Charleston, South Carolina.

Robotic intracorporeal anastomosis is currently increasingly used for the performance of colorectal anastomosis. We describe the technical details for the proper performance of an iso- and anti-peristaltic anastomosis including the set up of the robotic platform and the performance of the anastomosis. Read More

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September 2021

Balloon pulmonary angioplasty protocol in a Portuguese pulmonary hypertension expert center.

Rev Port Cardiol (Engl Ed) 2021 Sep;40(9):653-665

Cardiology Department, Hospital Garcia de Orta, Almada, Portugal; CCUL, CAML, University of Lisbon, Lisbon, Portugal.

Introduction: Balloon pulmonary angioplasty (BPA) has emerged as a promising therapeutic option for patients with chronic thromboembolic pulmonary hypertension (CTEPH) who are not eligible for pulmonary thromboendarterectomy (PEA) or who have recurrent or persistent pulmonary hypertension after surgery. There is no standardized technique for BPA and, its complexity and high risk of severe complications, requires skills and appropriate training and should be reserved for expert CTEPH centers, as a complementary intervention to medical and surgical therapy.

Objective: The purpose of this document is to describe the BPA protocol used at a high-volume center nationwide, validated by its results. Read More

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September 2021

Bridging stent-grafts fracture after branched endovascular aortic repair in a dual-center retrospective cohort study.

J Vasc Surg 2021 Sep 6. Epub 2021 Sep 6.

Department of Vascular Surgery, Ospedale Civile di Baggiovara, Azienda Ospedaliero-Universitaria di Modena, University of Modena and Reggio Emilia, Modena, Italy. Electronic address:

Purpose: Target vessel instability is a relevant complication after thoracoabdominal aortic aneurysms (TAAAs) branched endovascular aortic repair (BEVAR). Long-term bridging stent-grafts (BSGs) durability has already been established, but the incidence of long-term complications as component fractures was not deeply investigated. This paper aims to assess BSGs fracture incidence and risk factors after BEVAR. Read More

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September 2021


Ann Vasc Surg 2021 Sep 6. Epub 2021 Sep 6.

Department of Endovascular Therapy, Hospiten Hospital Group, Santa Cruz de Tenerife, Canary Island, Spain; University of Las Palmas de Gran Canarias (ULPGC), Las Palmas, Canary Island, Spain.

Introduction: Pelvic congestion syndrome (PCS) is a frequent finding in adult women and transcatheter embolization of dilated and refluxing veins is the treatment of choice. The procedure can be performed through different venous accesses such as the transfemoral, transjugular and the transbrachial access. The aim of this study was to demonstrate the feasibility and safety of the transbrachial approach for transcatheter embolization in this pathology in 201 women. Read More

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September 2021

Technical details for a robot-assisted hand-sewn esophago-gastric anastomosis during minimally invasive Ivor Lewis esophagectomy.

Surg Endosc 2021 Sep 9. Epub 2021 Sep 9.

Department of Surgery, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Background: Minimally invasive Ivor Lewis esophagectomy (MIILE) provides better outcomes than open techniques, particularly in terms of post-operative recovery and pulmonary complications. However, in addition to requiring advanced technical skills, thoracoscopic access makes it hard to perform esophagogastric anastomosis safely, and the reported rates of anastomotic leak vary from 5 to 16%. Several minimally invasive esophago-gastric anastomotic techniques have been described, but to date strong evidence to support one technique over the others is still lacking. Read More

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September 2021

3D culture system to study collective migration in mammary organoid epithelium.

STAR Protoc 2021 Sep 27;2(3):100778. Epub 2021 Aug 27.

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.

We recently established an culture system in which mammary gland organoid undergoes directional migration in response to an FGF10 concentration gradient. Here, we describe a step-by-step protocol for preparing organoids, the setup of the 3D culture system, and the image acquisition approach. The technical difficulties in conducting the 3D migration assay are choosing epithelial organoids of appropriate sizes and manually paring organoids and beads pre-soaked in FGF10 within a desirable distance (∼100 μm). Read More

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September 2021

Barbed Suture in Total Laparoscopic Hysterectomy: A Comparative Study of the Safety in Vaginal Cuff Closure with that of Polyglactin 910 Suture.

Gynecol Minim Invasive Ther 2021 Jul-Sep;10(3):154-158. Epub 2021 Aug 3.

Department of Obstetrics and Gynecology, JSS Medical College, JSS Academy of Higher Education & Research, Mysore, Karnataka, India.

Objectives: To evaluate whether the use of barbed sutures during laparoscopic hysterectomy for vaginal cuff closure has reduced the surgical difficulty and incidence of post-operative complications as compared to polyglactin 910 suture.

Materials And Methods: This is a randomized comparative study conducted at a tertiary care hospital and research institute where 100 patients were divided into two groups (50 each) and underwent vault closure using barbed suture/Polyglactin 910. Data collected include demographic details, indication for surgery, mean suturing time, degree of surgeon difficulty, and the incidence of postoperative complications when followed up to 12 weeks and were compared statistically using Chi square test and Independent-t test. Read More

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Concentration techniques tailored for the detection of SARS-CoV-2 genetic material in domestic wastewater and treatment plant sludge: A review.

J Environ Chem Eng 2021 Oct 30;9(5):106296. Epub 2021 Aug 30.

İstanbul Technical University, Civil Engineering Faculty, Environmental Engineering Department, Ayazağa Campus, Sarıyer, İstanbul 34469, Republic of Turkey.

Upon the outbreak of COVID-19 pandemic, detection and quantification of SARS-CoV-2 genetic material in domestic wastewater have led to an increase in the efforts to define and implement the wastewater-based epidemiology (WBE). This application provides valuable information to define local contamination monitoring, emergence of COVID-19 and its variants and many other aspects to cope with and control the pandemic. WBE surveillance, however, requires several consecutive steps such as sampling, pretreatment and concentration of samples, and detection and quantification of SARS-CoV-2 genetic material in wastewater. Read More

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October 2021

Early Outcomes of t-Branch Off-the-Shelf Multibranched Stent-Graft in Urgent and Emergent Repair of Thoracoabdominal Aortic Aneurysm.

J Vasc Surg 2021 Sep 1. Epub 2021 Sep 1.

German Aortic Center, Department of Vascular Medicine, University Heart & Vascular Center Hamburg, Germany.

Purpose: To compare outcomes between the elective, urgent, and emergent treatment of thoracoabdominal aortic aneurysms (TAAAs) with the t-branch off-the-shelf multibranched stent-graft.

Methods: All consecutive patients treated for TAAA using the t-Branch between September 2012 and June 2019 were included in the study. Patients were divided into three groups according to the urgency of repair; 1) elective, 2) urgent, and 3) emergent group. Read More

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September 2021

Current Perspectives on Contemporary Rheumatic Mitral Valve Repair.

Innovations (Phila) 2021 Sep 3:15569845211032942. Epub 2021 Sep 3.

1980 Department of Cardiac Surgery/Cardiovascular Sciences, University of Bristol, UK.

The surgical management of rheumatic mitral valve disease remains a challenge for cardiac surgeons. Durability of mitral valve repair (MVr) is likely compromised not simply due to high technical demand, but surgeon reluctance, despite boasting copious advantages over MV replacement. This comprehensive review aims to evoke a deeper understanding of MVr concepts necessary to abate these limitations and shift mindset towards a more holistic approach to repair. Read More

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September 2021

Technical and clinical validation of commercial automated volumetric MRI tools for dementia diagnosis-a systematic review.

Neuroradiology 2021 Sep 3. Epub 2021 Sep 3.

Department of Radiology and Nuclear Medicine, Erasmus MC University Medical Center, Rotterdam, The Netherlands.

Developments in neuroradiological MRI analysis offer promise in enhancing objectivity and consistency in dementia diagnosis through the use of quantitative volumetric reporting tools (QReports). Translation into clinical settings should follow a structured framework of development, including technical and clinical validation steps. However, published technical and clinical validation of the available commercial/proprietary tools is not always easy to find and pathways for successful integration into the clinical workflow are varied. Read More

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September 2021

Classifying patients with depressive and anxiety disorders according to symptom network structures: A Gaussian graphical mixture model-based clustering.

PLoS One 2021 1;16(9):e0256902. Epub 2021 Sep 1.

National Center for Cognitive Behavior Therapy and Research, National Center of Neurology and Psychiatry, Tokyo, Japan.

Patients with mental disorders often suffer from comorbidity. Transdiagnostic understandings of mental disorders are expected to provide more accurate and detailed descriptions of psychopathology and be helpful in developing efficient treatments. Although conventional clustering techniques, such as latent profile analysis, are useful for the taxonomy of psychopathology, they provide little implications for targeting specific symptoms in each cluster. Read More

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September 2021

Oil field-produced water treatment: characterization, photochemical systems, and combined processes.

Environ Sci Pollut Res Int 2021 Aug 31. Epub 2021 Aug 31.

Department of Chemical Engineering, Federal University of Rio Grande do Norte, Natal, 59078-970, Brazil.

Produced water, a mixture of inorganic and organic components, comprises the largest effluent stream from oil and gas activities. The removal of contaminants from this wastewater is receiving special attention of the researchers since most of them are persistent and difficult to remove with simple techniques. Several technologies from conventional to advanced oxidation processes have been employed to treat produced water. Read More

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Water Treatment for Hemodialysis: Keeping Patients Safe.

Nephrol Nurs J 2021 Jul-Aug;48(4):315-345

Managing Partner of Jim Curtis and Associates, LLC.

All nurses with any responsibility for hemodialysis need to know and understand the clinical ramifications of water treatment and dialysate preparation. Although some hemodialysis programs may have full-time technical staff who operate and test the water treatment and dialysate preparation systems, many programs rely on nursing staff for the day-to-day operation and testing of these systems. It is critical for all staff members to work together to keep patients safe. Read More

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September 2021

Objective follow-up after transection of uterine round ligament during laparoscopic repair of inguinal hernias in women: assessment of safety and long-term outcomes.

Surg Endosc 2021 Aug 30. Epub 2021 Aug 30.

Department of Colorectal Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, No. 107, Wenhua West Road, Jinan, 250012, Shandong Province, China.

Background: Whether to preserve the uterine round ligament during laparoscopic inguinal hernia repair in women is controversial. In this study, we aimed to compare outcomes of uterine round ligament preservation versus transection during such surgery and to explore the impact and long-term outcomes of transecting the round ligament.

Methods: The study cohort comprised 419 women who had undergone laparoscopic inguinal hernia repair in Beijing Chaoyang Hospital and Qilu Hospital from January 2013 to January 2020; 393 (93. Read More

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Pterygopalatine fossa schwannoma-case report and literature review.

Int J Surg Case Rep 2021 Sep 24;86:106312. Epub 2021 Aug 24.

Department of ENT & Maxillofacial Surgery, University of the West Indies, Port of Spain General Hospital, Trinidad and Tobago, W.I. Electronic address:

Introduction: Schwannomas are well-differentiated, benign tumours that originate from the Schwann cells of nerve sheaths. They constitute 25-45% of all the head and neck tumours and can cause significant morbidity depending on the site of origin. The pterygopalatine fossa is the rarest site of involvement, with only a few cases reported in the literature. Read More

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September 2021

3,4-Ethylenedioxythiophene (EDOT) End-Group Functionalized Poly-ε-caprolactone (PCL): Self-Assembly in Organic Solvents and Its Coincidentally Observed Peculiar Behavior in Thin Film and Protonated Media.

Polymers (Basel) 2021 Aug 14;13(16). Epub 2021 Aug 14.

Centre of Advanced Research in Bionanoconjugates and Biopolymers, "Petru Poni" Institute of Macromolecular Chemistry, 41A, Grigore-Ghica Voda Alley, 700487 Iasi, Romania.

End-group functionalization of homopolymers is a valuable way to produce high-fidelity nanostructured and functional soft materials when the structures obtained have the capacity for self-assembly (SA) encoded in their structural details. Herein, an end-functionalized PCL with a π-conjugated EDOT moiety, (), designed exclusively from hydrophobic domains, as a functional "hydrophobic amphiphile", was synthesized in the bulk ROP of ε-caprolactone. The experimental results obtained by spectroscopic methods, including NMR, UV-vis, and fluorescence, using DLS and by AFM, confirm that in solvents with extremely different polarities (chloroform and acetonitrile), presents an interaction- and structure-based bias, which is strong and selective enough to exert control over supramolecular packing, both in dispersions and in the film state. Read More

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Using Virtual Scanning to Find Optimal Configuration of a 3D Scanner Turntable for Scanning of Mechanical Parts.

Sensors (Basel) 2021 Aug 7;21(16). Epub 2021 Aug 7.

Faculty of Mechanical Engineering, Technical University of Košice, 04200 Košice, Slovakia.

The article describes a method of simulated 3D scanning of triangle meshes based on ray casting which is used to find the optimal configuration of a real 3D scanner turntable. The configuration include the number of scanners, their elevation above the rotary table and the number of required rotation steps. The evaluation is based on the percentage of the part surface covered by the resulting point cloud, which determines the ability to capture all details of the shape. Read More

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Bilayer versus Polymeric Artificial Water Channel Membranes: Structural Determinants for Enhanced Filtration Performances.

J Am Chem Soc 2021 Sep 27;143(35):14386-14393. Epub 2021 Aug 27.

Lehn Institute of Functional Materials, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

Artificial water channels (AWCs) and their natural aquaporin counterparts selectively transport water. They represent a tremendous source of inspiration to devise biomimetic membranes for several applications, including desalination. They contain variable water-channel constructs with adaptative architectures and morphologies. Read More

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September 2021

Bilateral Lung Transplantation for Patients With Destroyed Lung and Asymmetric Chest Deformity.

Front Surg 2021 10;8:680207. Epub 2021 Aug 10.

Wuxi Lung Transplant Center, Department of Thoracic Surgery, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.

Destroyed lung can cause mediastinal displacement and asymmetric chest deformity. Reports on bilateral lung transplantation (LT) to treat destroyed lung and asymmetric chest deformity are rare. This study presents our surgical experience of bilateral LT among patients with destroyed lung and asymmetric chest deformity. Read More

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The combined use of thoracic and abdominal aortic stent grafts for endovascular repair of wide neck abdominal aortic aneurysms in high risk patients.

Ann Vasc Surg 2021 Aug 23. Epub 2021 Aug 23.

Department of Endovascular and Vascular Surgery, Geisinger Medical Center, Danville, PA.

Introduction: Treatment of abdominal aortic aneurysms (AAA) with large (28 mm to 34 mm) and wide diameter (> 35 mm) necks remains a challenge in patients who are high-risk candidates for open repair. While several case reports describe the use of a thoracic stent graft in conjunction with a traditional modular bifurcated stent graft, most patients do not have the aortic length to accommodate such a configuration. We present our experience utilizing a distal unibody bifurcated aortic stent graft (Endologix, Irvine, CA) in conjunction with a proximal thoracic aortic stent graft (Medtronic, Minneapolis, MN) to treat wide-necked non-ruptured AAAs in patients who were otherwise poor candidates for open or fenestrated repair. Read More

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AngioVac Aspiration Thrombectomy of Right Atrial Thrombus is Safe and Effective in Cancer Patients.

Ann Vasc Surg 2021 Aug 23. Epub 2021 Aug 23.

Department of Radiology, Division of Interventional Radiology, University of California at Los Angeles, Los Angeles, CA.

Objectives: The aim of this study was to test the hypothesis that endovascular aspiration thrombectomy of right atrial thrombus (RAT) using the AngioVac device is as safe and effective in patients with cancer as those without cancer.

Background: RAT is a uniquely challenging clinical presentation of venous thromboembolism due to its low incidence and historically high-risk of mortality due to thrombus propagation into the pulmonary arteries. There is a lack of consensus regarding management, particularly in high-risk cancer patients. Read More

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