Stem Cell Reports 2021 Sep 8. Epub 2021 Sep 8.
Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, CB2 0AW Cambridge, UK; Lab of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, Greece. Electronic address:
Postnatal brain neural stem and progenitor cells (NSPCs) cluster in anatomically inaccessible stem cell niches, such as the subependymal zone (SEZ). Here, we describe a method for the isolation of NSPCs from live animals, which we term "milking." The intracerebroventricular injection of a release cocktail, containing neuraminidase, integrin-β1-blocking antibody, and fibroblast growth factor 2, induces the controlled flow of NSPCs in the cerebrospinal fluid, where they are collected via liquid biopsies. Read More