269,206 results match your criteria target gene

Ectopic expression of MELK in oral squamous cell carcinoma and its correlation with epithelial mesenchymal transition.

Aging (Albany NY) 2021 May 7;13. Epub 2021 May 7.

Xiangya School of Stomatology, Central South University, Changsha, Hunan 410008, PR China.

Epithelial-mesenchymal transition (EMT) is closely correlated to metastasis formation generation and maintenance of cancer stem cells, nevertheless, the underlying mechanisms are unclear. The aim of this study is to investigate the role of maternal embryonic leucine-zipper kinase (MELK) in EMT regulation in oral squamous cell carcinoma (OSCC). We found that there was overexpression of MELK in human OSCC tissues, and high MELK expression was correlated with lymphatic metastasis and led to poor prognosis in patients with OSCC. Read More

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Family with sequence similarity 83 member A promotes tumor cell proliferation and metastasis and predicts poor prognosis in cervical cancer.

Pathol Res Pract 2021 Apr 15;222:153450. Epub 2021 Apr 15.

Department of Obstetrics and Gynecology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China. Electronic address:

Family with sequence similarity 83 member A (FAM83A) is a member of the FAM83 family and is proven to have oncogenic properties in several cancers. However, the mechanisms of FAM83A in human cervical cancer (CC) progression are unknown. Here, we found that FAM83A is highly expressed in CC tissues and cell lines through western blot and qRT-PCR. Read More

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IL-6 regulates the bone metabolism and inflammatory microenvironment in aging mice by inhibiting Setd7.

Acta Histochem 2021 May 4;123(5):151718. Epub 2021 May 4.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Lingyuan West Road, Guangzhou, Guangdong, 510055, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China; Guangdong Engineering Research Center of Technology and Materials for Oral Reconstruction, Guangzhou, China. Electronic address:

Aging, which has become a worldwide problem, leads to the degeneration of multiple organs and tissues. Two of the main changes in aging are dysregulation of the tissue microenvironment and abnormal functioning of specific stem cells. Bone marrow stem cells (BMSCs) in the aging microenvironment are not only effector cells but also immunomodulatory cells that change the microenvironment. Read More

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Transcriptome analysis provides insights into long noncoding RNAs in medaka gonads.

Comp Biochem Physiol Part D Genomics Proteomics 2021 Apr 29;39:100842. Epub 2021 Apr 29.

International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

Long non-coding RNAs (lncRNAs) are gradually regarded as regulators in sex determination and gonad development of various animals. Medaka (Oryzias latipes) is an excellent reproductive research model with sex-determining genes. However, the regulation of gonadal lncRNAs on medaka reproductive development remains unknown. Read More

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Proteomic analysis of liver tissues in chicken embryo at Day 16 and Day 20 reveals antioxidant mechanisms.

J Proteomics 2021 May 4:104258. Epub 2021 May 4.

College of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China. Electronic address:

To investigate the mechanisms of the defense system and antioxidant defense system during chicken embryo development, protein profiling of liver tissues in chicken embryo at Day 16 and Day 20 was conducted. TMT was used to analyze the liver tissues proteomes with significantly different activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in chicken embryo. PRM was operated to validate the target differentially abundant proteins (DAPs) using the same samples. Read More

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Osteoarthritis: from upcoming treatments to treatments yet to come.

Joint Bone Spine 2021 May 4:105206. Epub 2021 May 4.

Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, F-44000 Nantes, France; Université de Nantes, UFR Odontologie, F-44042 Nantes, France.

Osteoarthritis affects hundreds of millions of people worldwide, and its prevalence is constantly increasing. While there is currently no treatment that can alter the course of the disease, promising therapeutic strategies and novel targets are being investigated. Innovative cell therapies are already reaching clinical trials, and recent progress in our understanding of the disease is opening new routes for gene therapy. Read More

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The potential endocrine disruption mechanism of anthelmintic drug niclosamide by activating estrogen receptors and estrogen-related receptors.

Toxicology 2021 May 4:152805. Epub 2021 May 4.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, PR China. Electronic address:

Niclosamide (NIC), a helminthic drug used widely for controlling schistosomiasis, can reportedly disrupt the endocrine system. However, its underlying mechanisms are still unclear. In this study, we revealed the potential endocrine disruption mechanism of NIC by activating estrogen receptors (ERs) and estrogen-related receptors (ERRs). Read More

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Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway.

Toxicology 2021 May 4:152806. Epub 2021 May 4.

Section of Biomolecular Medicine. Electronic address:

Colorectal cancer (CRC) is the third most common cancer worldwide and the second leading cause of cancer death. Benzo[a]pyrene (BaP) and 2-amino-1-methyl-6-phenylimidazol [4,5-b] pyridine (PhIP) present in cooked meat are pro-carcinogens and considered to be potential risk factors for CRC. Their carcinogenic and mutagenic effects require metabolic activation primarily by cytochrome P450 1 family enzymes (CYPs); the expression of these enzymes can be modulated by aryl hydrocarbon receptor (AhR) activation and the tumour microenvironment, involving mediators of inflammation. Read More

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TIRR inhibits the 53BP1-p53 complex to alter cell-fate programs.

Mol Cell 2021 Apr 29. Epub 2021 Apr 29.

Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA. Electronic address:

53BP1 influences genome stability via two independent mechanisms: (1) regulating DNA double-strand break (DSB) repair and (2) enhancing p53 activity. We discovered a protein, Tudor-interacting repair regulator (TIRR), that associates with the 53BP1 Tudor domain and prevents its recruitment to DSBs. Here, we elucidate how TIRR affects 53BP1 function beyond its recruitment to DSBs and biochemically links the two distinct roles of 53BP1. Read More

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AHNAK controls 53BP1-mediated p53 response by restraining 53BP1 oligomerization and phase separation.

Mol Cell 2021 Apr 29. Epub 2021 Apr 29.

Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch, France; Centre National de Recherche Scientifique, UMR7104, 67404 Illkirch, France; Université de Strasbourg, 67081 Strasbourg, France. Electronic address:

p53-binding protein 1 (53BP1) regulates both the DNA damage response and p53 signaling. Although 53BP1's function is well established in DNA double-strand break repair, how its role in p53 signaling is modulated remains poorly understood. Here, we identify the scaffolding protein AHNAK as a G1 phase-enriched interactor of 53BP1. Read More

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Whole transcriptome analysis of the differential RNA profiles and associated competing endogenous RNA networks in LPS-induced acute lung injury (ALI).

PLoS One 2021 7;16(5):e0251359. Epub 2021 May 7.

School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Acute lung injury (ALI) is a serious inflammation disease usually arises alveolar epithelial membrane dysfunction and even causes death. Therefore, the aims of this study are to screen the differentially expressed lncRNAs, circRNAs, miRNAs, and mRNAs in ALI based on the high-throughput sequencing. The lipopolysaccharide (LPS)-induced ALI mouse model was established, the injury of ALI mouse model was evaluated through histological analysis with hemotoxylin and eosin (H & E) staining assay, dry/wet ratio, infiltrated-immune cells, ET-1 mRNA expression and released-proinflammation factors. Read More

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Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2.

PLoS Biol 2021 May 7;19(5):e3001236. Epub 2021 May 7.

Murphy Medical Associates, Greenwich, Connecticut, United States of America.

With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. It was found that the B.1. Read More

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MiR-485-3p promotes proliferation of osteoarthritis chondrocytes and inhibits apoptosis via Notch2 and the NF-κB pathway.

Immunopharmacol Immunotoxicol 2021 May 7:1-10. Epub 2021 May 7.

Department of Foot and Ankle Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Context: Osteoarthritis (OA) is one of the leading causes of disability worldwide. microRNAs (miRs) has been shown to be involved in multiple pathological processes during OA. But the possible mechanism of miR-485-3p in OA remains unclear. Read More

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EPO transgene detection in Dried Blood Spots for antidoping application.

Drug Test Anal 2021 May 7. Epub 2021 May 7.

Analysis Department - Agence Française de Lutte contre le Dopage (AFLD), 143 avenue Roger Salengro-, 92290, Châtenay-Malabry, France.

The modification of gene expression to treat diseases is a field of research with exponential growth. As doping in sport closely follows emerging therapies, a surveillance of the modification of gene expression to enhance performance is needed. The gene coding for erythropoietin (EPO), is one target of interest. Read More

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Overexpression of miR-7977 in CD4+ T cells is associated with multiplex autoimmunity in patients with Addison's disease.

Eur J Endocrinol 2021 May 1. Epub 2021 May 1.

M Ruchala, Department of Endocrinology, Metabolism and Internal Medicine, University of Medical Sciences, Poznan, 60-355, Poland.

Objective: Autoimmune Addison's disease (AD) results from a combination of the genetic predisposition, unclear environmental triggers and ensuing immune dysfunction. MicroRNA molecules (miRNAs) are involved in post-transcriptional regulation of numerous target genes, hence may affect the immune function and promote autoimmunity. A deregulated miRNAs profile was reported in several autoimmune conditions. Read More

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The lipolytic activity of LipJ, a stress-induced enzyme, is regulated by its C-terminal adenylate cyclase domain.

Future Microbiol 2021 May 7. Epub 2021 May 7.

Department of Biotechnology, BMS Block-1, South Campus, Panjab University, Chandigarh, India.

The confirmation of lipolytic activity and role of Rv1900c in the mycobacterium physiology /N-terminus domain () were cloned in pET28a/, purified by affinity chromatography and characterized. A zone of clearance on tributyrin-agar and activity with NP-decanoate confirmed the lipolytic activity of Rv1900c. The Rv1900NT demonstrated higher enzyme specific activity, V and , but Rv1900c was more thermostable. Read More

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Identification of autophagy-related genes signature predicts chemotherapeutic and immunotherapeutic efficiency in bladder cancer (BLCA).

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Internal Medicine, Division of Nephrology, Leiden University Medical Center, Leiden, The Netherlands.

Autophagy maintains cellular homeostasis by degrading and recycling cytoplasmic components under stress conditions, which is identified to be involved in tumorigenesis and now has been recognized as novel target in cancer treatment. In present study, we gathered total autophagy-related genes and established an autophagy-related genes signature (ATGRS) through LASSO cox regression analysis in BLCA. Kaplan-Meier survival and multivariate cox regression analyses both showed the ATGRS was a robust independent prognostic factor with high accuracy. Read More

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Microdeletion of 9q22.3: A patient with minimal deletion size associated with a severe phenotype.

Am J Med Genet A 2021 May 7. Epub 2021 May 7.

Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Queensland, Australia.

Basal cell nevus syndrome (also known as Gorlin Syndrome; MIM109400) is an autosomal dominant disorder characterized by recurrent pathological features such as basal cell carcinomas and odontogenic keratocysts as well as skeletal abnormalities. Most affected individuals have point mutations or small insertions or deletions within the PTCH1 gene on human chromosome 9, but there are some cases with more extensive deletion of the region, usually including the neighboring FANCC and/or ERCC6L2 genes. We report a 16-year-old patient with a deletion of approximately 400,000 bases which removes only PTCH1 and some non-coding RNA genes but leaves FANCC and ERCC6L2 intact. Read More

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ZEB1 serves as an oncogene in acute myeloid leukaemia via regulating the PTEN/PI3K/AKT signalling pathway by combining with P53.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

The Key Laboratory of Major Autoimmune Diseases of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, China.

Acute myeloid leukaemia is a complex, highly aggressive hematopoietic disorder. Currently, in spite of great advances in radiotherapy and chemotherapy, the prognosis for AML patients with initial treatment failure is still poor. Therefore, the need for novel and efficient therapies to improve AML treatment outcome has become desperately urgent. Read More

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Integrated microenvironment-associated genomic profiles identify LRRC15 mediating recurrent glioblastoma-associated macrophages infiltration.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, China.

Glioblastoma (GBM) is the most common malignant intracranial tumour with intrinsic infiltrative characteristics, which could lead to most patients eventually relapse. The prognosis of recurrent GBM patients remains unsatisfactory. Cancer cell infiltration and their interaction with the tumour microenvironment (TME) could promote tumour recurrence and treatment resistance. Read More

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Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway.

J Cell Mol Med 2021 May 7. Epub 2021 May 7.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.

Asthma is a chronic airway disease that causes excessive inflammation, oxidative stress, mucus production and bronchial epithelial cell apoptosis. Fructose-1,6-bisphosphatase (Fbp1) is one of the rate-limiting enzymes in gluconeogenesis and plays a critical role in several cancers. However, its role in inflammatory diseases, such as asthma, is unclear. Read More

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Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba.

Tree Physiol 2021 May 7. Epub 2021 May 7.

School of Forest Resources and Conservation, University of Florida, Gainesville, FL 32611, USA.

Although the CRISPR/Cas9 system has been successfully used for crop breeding, its application remains limited in forest trees. Here we describe an efficient gene editing strategy for hybrid poplar, based on the Golden Gate MoClo cloning. To test the system efficiency for generating single gene mutants, two sgRNAs were designed and incorporated into the MoClo Tool Kit level 2 binary vector with the Cas9 expression cassette to mutate SHORT ROOT (SHR) gene. Read More

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Network pharmacology-based identification of the key mechanism of quercetin acting on hemochromatosis.

Metallomics 2021 May 7. Epub 2021 May 7.

College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, 310058, China.

Hemochromatosis is an iron overload disease, which lacks nutritional intervention strategies. This study explored the protective effect of quercetin on hemochromatosis and its possible mechanism through network pharmacology. We used Online Mendelian Inheritance in Man (OMIM) to screen the disease targets of hemochromatosis, and further constructed a potential protein interaction network through STITCH. Read More

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Identification of biomarkers related to Tumor-Infiltrating Lymphocytes (TILs) infiltration with gene co-expression network in colorectal cancer.

Bioengineered 2021 Dec;12(1):1676-1688

Department of Abdominal Oncology, West China Hospital of Sichuan University, Chengdu, Sichuan, P.R. China.

Colorectal cancer (CRC) is one of the most common tumors, ranking second in the global cause of death from cancer. The prognosis of advanced patients is still very poor. In this study, hub modules with the highest association with tumor-infiltrating immune cells were identified by weighted gene co-expression network analysis based on CRC expression data from the Gene Expression Omnibus database. Read More

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December 2021

Relationship between hepatocellular carcinoma and depression via online database analysis.

Bioengineered 2021 Dec;12(1):1689-1697

Clinical laboratory, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

There may be a mutually reinforcing relationship between hepatocellular carcinoma (HCC) and depression, but the mechanism is unknown. This study used bioinformatics to evaluate the relationship between HCC and depression at the genetic level. Genes associated with HCC and depression were obtained from pubmed2ensemble. Read More

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December 2021

The comprehensive landscape of miR-34a in cancer research.

Cancer Metastasis Rev 2021 May 6. Epub 2021 May 6.

New Drug Screening Center, China Pharmaceutical University, Nanjing, 210009, China.

MicroRNA-34 (miR-34) plays central roles in human diseases, especially cancers. Inactivation of miR-34 is detected in cancer cell lines and tumor tissues versus normal controls, implying its potential tumor-suppressive effect. Clinically, miR-34 has been identified as promising prognostic indicators for various cancers. Read More

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MicroRNA-26 regulates the expression of CTGF after exposure to ionizing radiation.

Radiat Environ Biophys 2021 May 6. Epub 2021 May 6.

Department of Matrix Biology and Medicine, Faculty of Medicine, Oita University, Oita, Japan.

Radiation-induced fibrosis (RIF) is a serious complication that occurs after irradiation and which is caused by the deposition of extracellular matrix (ECM) proteins such as collagen. However, the underlying mechanisms, including the expression of the cytokines, that promote the RIF process, are not yet fully understood. MicroRNAs (miRNAs) have recently been suggested to act as post-transcriptional repressors for many genes; however, their role in the process of RIF remains to be elucidated. Read More

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Immunological networks defining the heterogeneity of inflammatory bowel diseases.

J Crohns Colitis 2021 May 7. Epub 2021 May 7.

Division of immunology and Allergy, Department of Medicine, Solna, Karolinska Institutet and University Hospital, 17176 Stockholm, Sweden.

Current practice in IBD is to classify patients based on clinical signs and symptoms and provide treatments accordingly. However, the response of IBD patients to available treatments is highly variable, highlighting clinically significant heterogeneity among patients. Thus, more accurate patient stratification is urgently needed to more effectively target therapeutic interventions to specific patients. Read More

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Hypoxia Constructing the Prognostic Model of Colorectal Adenocarcinoma and Related to the Immune Microenvironment.

Front Cell Dev Biol 2021 20;9:665364. Epub 2021 Apr 20.

Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Hypoxia is a common phenomenon in solid tumors, which plays an important role in tumor proliferation, apoptosis, angiogenesis, invasion and metastasis, energy metabolism and chemoradiotherapy resistance. However, comprehensive analysis of hypoxia markers in colorectal adenocarcinoma (COAD) is still lacking. And there is a need for mechanism exploration and clinical application. Read More

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Reck-Notch1 Signaling Mediates miR-221/222 Regulation of Lung Cancer Stem Cells in NSCLC.

Front Cell Dev Biol 2021 20;9:663279. Epub 2021 Apr 20.

Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Cancer stem cells (CSCs) contribute to the cancer initiation, metastasis and drug resistance in non-small cell lung cancer (NSCLC). Herein, we identified a miR-221/222 cluster as a novel regulator of CSCs in NSCLC. Targeted overexpression or knockdown of miR-221/222 in NSCLC cells revealed the essential roles of miR-221/222 in regulation of lung cancer cell proliferation, mammosphere formation, subpopulation of CD133 CSCs and the expression of stemness genes including OCT4, NANOG and h-TERT. Read More

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