6,538 results match your criteria taqman real-time


Photobiomodulation Therapy on Myocardial Infarction in Rats: Transcriptional and Posttranscriptional Implications to Cardiac Remodeling.

Lasers Surg Med 2021 Apr 13. Epub 2021 Apr 13.

Universidade Nove de Julho, Rua Vergueiro 249, Liberdade, São Paulo, SP, 01504-001, Brazil.

Background And Objectives: Induction of myocardial infarction (MI) in rats by occlusion of the left anterior descending coronary artery is an experimental model used in research to elucidate functional, structural, and molecular modifications associated with ischemic heart disease. Photobiomodulation therapy (PBMT) has become a therapeutic alternative by modulating various biological processes eliciting several effects, including anti-inflammatory and pro-proliferative actions. The main objective of this work was to evaluate the effect of PBMT in the modulation of transcriptional and post-transcriptional changes that occurred in myocardium signal transduction pathways after MI. Read More

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in cattle and their human contacts in a gaushala (cattle shelter) from India and its partial 1 gene sequence-based phylogenetic analysis.

Anim Biotechnol 2021 Apr 12:1-10. Epub 2021 Apr 12.

Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India.

Q fever caused by is an important zoonosis and has great public health significance. A total of 905 clinical samples from 387 cattle [serum ( = 387); vaginal swabs ( = 387); milk ( = 131)] and 59 serum samples from humans were collected from gaushala (cattle shelter) and screened for anti- IgG antibodies in the sera using an indirect-ELISA kit. Further, the samples were tested for DNA employing TaqMan real-time and conventional PCR assays targeting the 1 gene. Read More

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Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of .

Curr Med Mycol 2020 Sep;6(3):50-56

Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Background And Purpose: is an emerging multidrug-resistant pathogen. The identification of this species with the conventional phenotypic or biochemical mycological methods may lead to misidentification. Molecular-based species-specific identification methods such as quantitative real-time polymerase chain reaction (qPCR) facilitate a more reliable identification of than mycological methods. Read More

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September 2020

Development and application of a TaqMan-based real-time PCR assay for specifically detecting feline astrovirus.

Mol Cell Probes 2021 Apr 6;57:101729. Epub 2021 Apr 6.

College of Veterinary Medicine, Jilin Agricultural University, Changchun, Jilin, 130118, China. Electronic address:

Feline astrovirus (FeAstV), an enteric RNA virus of recent concern that is associated with diarrheal illness in cats, has been described in several countries throughout the world. However, no scientific and sensitive diagnostic method against FeAstV was reported up to now. Here, we developed a specific, sensitive and repeatable TaqMan fluorescence quantitative PCR (qPCR) assay to investigate the prevalence of FeAstV in domestic cats from China, especially low copy numbers in clinical sample. Read More

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Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR.

Virol J 2021 Apr 7;18(1):70. Epub 2021 Apr 7.

Division of Virology, Department of Veterinary Sciences, LMU Munich, Veterinärstrasse 13, 80539, Munich, Germany.

Background: The porcine circovirus type 2 (PCV2) is divided into eight genotypes including the previously described genotypes PCV2a to PCV2f and the two new genotypes PCV2g and PCV2h. PCV2 genotyping has become an important task in molecular epidemiology and to advance research on the prophylaxis and pathogenesis of PCV2 associated diseases. Standard genotyping of PCV2 is based on the sequencing of the viral genome or at least of the open reading frame 2. Read More

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Detection of Pneumocystis jirovecii from Clinical Specimens Utilizing a TaqMan-Based Real-Time PCR Assay on the Luminex ARIES.

Curr Protoc 2021 Apr;1(4):e95

Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.

Pneumocystis jirovecii can cause severe pneumonia in immunocompromised patients, which can be life threatening if left untreated. Despite the widespread use of polymerase chain reaction (PCR) within the clinical laboratory setting, FDA-approved PCR assays are not readily available for the detection of Pneumocystis from respiratory samples. Using the Luminex ARIES system-an open-channel, automated, sample-to-answer PCR platform-the cell division cycle 2 (cdc-2) gene can be targeted for the detection of Pneumocystis. Read More

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Influence of Potential Gene Polymorphisms on Propofol Dosage Regimen in Patients Undergoing Abdominal Hysterectomy.

Balkan J Med Genet 2020 Nov 23;23(2):41-48. Epub 2021 Mar 23.

University Clinic for Gynecology and Obstetrics, University "Ss Cyril and Methodius" Medical Faculty, Skopje, Republic of North Macedonia.

Propofol (2,6-diisopropylphenol) is the most common intravenous anesthetic used in modern medicine. It is postulated that individual differences in genetic factors [polymorphism of single nucleotide polymorphisms (SNPs)] in the genes encoding metabolic enzymes, molecular targets and molecular binding sites of propofol can be responsible for susceptibility to propofol effects. The aim of our study was to investigate the influence of the cytochrome P450 2B6 isozyme (rs3745274), γ-aminobutyric acid type A (GABAA) receptor α1 subunit (rs2279020) and ATP-binding cassette subfamily B member 1 (rs1045642) gene polymorphisms on propofol therapeutic outcomes in the patients undergoing abdominal hysterectomy. Read More

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November 2020

Serum Extracellular Vesicle-Derived miRNAs in Patients with Non-Small Cell Lung Cancer-Search for Non-Invasive Diagnostic Biomarkers.

Diagnostics (Basel) 2021 Mar 3;11(3). Epub 2021 Mar 3.

Department of Biomedicine and Genetics, Medical University of Lodz, 92-213 Lodz, Poland.

The aim of the study was a search for diagnostic and/or prognostic biomarkers in patients with non-small cell lung cancer (NSCLC) patients, based on circulating microRNAs (miRs: miR-23a, miR-361, miR-1228 and miR-let7i) in extracellular vesicles (EVs). Serum EVs were isolated from NSCLC patients ( = 31) and control subjects ( = 21). RNA was isolated from EVs and reverse transcription reaction was performed. Read More

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Diagnosis and Monitoring of Hepatitis B Virus Infection Using the Cobas HBV Test for Use on the Cobas 4800 System.

Microorganisms 2021 Mar 11;9(3). Epub 2021 Mar 11.

National Reference Center for Viral Hepatitis B, C and delta, Department of Virology, hôpital Henri Mondor, Université Paris-Est, 94000 Créteil, France.

(1) Background: Sensitive and accurate nucleic acid amplification technologies are now recommended for hepatitis B virus (HBV) DNA detection and quantification in clinical practice to diagnose and monitor hepatitis B infection. The aim of this study was to assess the analytical and clinical performance of the cobas HBV Test on the cobas 4800 System. (2) Methods: Standard panel and clinical specimens were tested in parallel with three different real-time commercial PCR assays including the cobas HBV Test, the Cobas AmpliPrep/Cobas TaqMan HBV Test v2. Read More

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Development of singleplex and multiplex real-time (Taqman®) RT-PCR assays for the detection of viruses associated with fig mosaic disease.

J Virol Methods 2021 Mar 30;293:114145. Epub 2021 Mar 30.

Istituto Agronomico Mediterraneo di Bari (CIHEAM-IAMB), Via Ceglie 9, 70010, Valenzano, Bari, Italy. Electronic address:

Singleplex and multiplex real-time (TaqMan®) RT-PCR assays were developed to detect seven fig-infecting viruses, i.e., fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV), fig mosaic virus (FMV), fig latent virus 1 (FLV-1), fig cryptic virus 1 (FCV-1) and fig fleck-associated virus (FFkaV). Read More

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Glutathione S-Transferase ( rs17856199) and Nitric Oxide Synthase ( rs2297518) Genotype Combination as Potential Oxidative Stress-Related Molecular Markers for Type 2 Diabetes Mellitus.

Diabetes Metab Syndr Obes 2021 25;14:1385-1403. Epub 2021 Mar 25.

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Background: Deregulation of the antioxidant enzymes was implicated in pathogenesis and complications of type 2 diabetes mellitus (T2DM). The data relate the genetic variants of these enzymes to T2DM are inconsistent among various populations.

Purpose: We aimed to explore the association of 13 genetic variants of "superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and nitric oxide synthase (NOS)" with T2DM susceptibility and the available clinical laboratory data. Read More

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Association of F11R polymorphisms and gene expression with primary Sjögren's syndrome patients.

Int J Rheum Dis 2021 Mar 29. Epub 2021 Mar 29.

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Aims: F11R gene encodes junctional adhesion molecule-A protein (JAM-A), which is expressed in various types of cells and is involved in leukocyte extravasation during inflammation. Sjögren's syndrome (SS) is a chronic systemic inflammatory disease that involves lymphocytes invasion of exocrine glands. F11R has been studied in autoimmune diseases, but any association between F11R and SS has not yet been investigated. Read More

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Overexpression of endogenous retroviruses in children with celiac disease.

Eur J Pediatr 2021 Mar 26. Epub 2021 Mar 26.

Department of Public Health and Pediatric Sciences, University of Turin, Piazza Polonia, 96 10126, Turin, Italy.

Human endogenous retroviruses (HERVs) represent 8% of our genome. Although no longer infectious, they can regulate transcription of adjacent cellular genes, produce retroviral RNAs, and encode viral proteins that can modulate both innate and adaptive immune responses. Based on this, HERVs have been studied and proposed as contributing factors in various autoimmune disorders. Read More

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Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus.

BMC Vet Res 2021 Mar 23;17(1):128. Epub 2021 Mar 23.

Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.

Background: Morbilliviruses are categorized under the family of Paramyxoviridae and have been associated with severe diseases, such as Peste des petits ruminants, canine distemper and measles with evidence of high morbidity and/or could cause major economic loss in production of livestock animals, such as goats and sheep. Feline morbillivirus (FeMV) is one of the members of Morbilliviruses that has been speculated to cause chronic kidney disease in cats even though a definite relationship is still unclear. To date, FeMV has been detected in several continents, such as Asia (Japan, China, Thailand, Malaysia), Europe (Italy, German, Turkey), Africa (South Africa), and South and North America (Brazil, Unites States). Read More

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Postpartum circulating microRNA enhances prediction of future type 2 diabetes in women with previous gestational diabetes.

Diabetologia 2021 Mar 23. Epub 2021 Mar 23.

Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, University of Melbourne, Heidelberg, VIC, Australia.

Aims/hypothesis: Type 2 diabetes mellitus is a major cause of morbidity and death worldwide. Women with gestational diabetes mellitus (GDM) have greater than a sevenfold higher risk of developing type 2 diabetes in later life. Accurate methods for postpartum type 2 diabetes risk stratification are lacking. Read More

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Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR-a pilot study.

BMC Res Notes 2021 Mar 9;14(1):90. Epub 2021 Mar 9.

Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.

Objective: Prompt diagnosis of Helicobacter pylori infection is essential for proper treatment and eradication of the pathogen because prolonged infection could lead to gastric cancer. Sensitive and cost effective diagnostic methods are key to guiding treatment options that will reduce mortality. This study was aimed at detecting H. Read More

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Evaluation of influenza A and B cold-adapted reassortant virus reproduction in trivalent live influenza vaccines.

Virus Res 2021 Mar 17:198396. Epub 2021 Mar 17.

Federal State Budget Scientific Institution "Institute of Experimental Medicine", St. Petersburg, Russian Federation.

Background: The objective of the present study was to compare reproduction of trivalent LAIV vaccine strains in MDCK cells and to perform quantitative RT-PCR analysis of trivalent LAIV replication after inoculation in mice.

Methods: We applied a reverse transcriptase real-time PCR (rRT-PCR) analysis using TaqMan technique to evaluate the infectious titers of vaccine strains containing in trivalent live influenza vaccines (LAIVs). We confirmed the PCR data in ELISA using staining of MDCK monolayer with mouse monoclonal antibodies to hemagglutinin. Read More

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The Chicken Egg Chorioallantoic Membrane (CAM) Model as an In Vivo Method for the Investigation of the Invasion and Metastasis Cascade of Malignant Tumor Cells.

Methods Mol Biol 2021 ;2294:17-26

Department of Experimental Surgery - Cancer Metastasis, Medical Faculty Mannheim, Ruprecht Karls University of Heidelberg, Mannheim, Germany.

The CAM model enables an in vivo analysis of the individual sub-steps of the metastatic cascade like local invasion, intravasation, or the establishment of metastasis in particular organs. Incubated fertilized chicken eggs are inoculated with human tumor cells and further processed for up to 9-10 days. The invasion and metastasis of these cells is then detected quantitatively with high specificity and sensitivity by means of a PCR for human ALU sequences, using the genomic DNA isolated from distant portions of the CAM, as well as from diverse internal organs of the developing embryo. Read More

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January 2021

YTHDC1 gene polymorphisms and Wilms tumor susceptibility in Chinese children: A five-center case-control study.

Gene 2021 May 16;783:145571. Epub 2021 Mar 16.

Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, Guangdong, China. Electronic address:

Wilms tumor is a common pediatric tumor with abundant genetic drivers. YTHDC1 is an important reader of the N-methyladenosine modification that widely regulates eukaryotic transcripts. YTHDC1 has been associated with the occurrence and development of some tumors. Read More

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Bisulfite-Converted DNA Quantity Evaluation: A Multiplex Quantitative Real-Time PCR System for Evaluation of Bisulfite Conversion.

Front Genet 2021 25;12:618955. Epub 2021 Feb 25.

Department of Forensic Medicine and Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea.

Bisulfite (BS) conversion, which includes a series of chemical reactions using bisulfite, is a prerequisite to most DNA methylation analysis methods, and thus is an essential step in the associated research process. Unfortunately, BS conversion leads to the degradation or loss of DNA, which can hinder further downstream analysis. In addition, it is well known that incomplete BS conversion is crucial, as it causes an exaggeration of the DNA methylation level, which can adversely affect the results. Read More

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February 2021

Development of a Rapid and Fully Automated Multiplex Real-Time PCR Assay for Identification and Differentiation of and on the BD MAX Platform.

Front Cell Infect Microbiol 2021 25;11:639473. Epub 2021 Feb 25.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

and are common diarrheal pathogens of great public health concern. A multiplex TaqMan-based real-time PCR assay was developed on the BD MAX platform; this assay can simultaneously detect and differentiate and directly from human fecal specimens. The assay includes two reactions. Read More

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February 2021

The impact of mitochondria-related and variants on breast cancer pathomorphological characteristics and patient outcomes.

Biomarkers 2021 Mar 26:1-11. Epub 2021 Mar 26.

Institute of Oncology, Lithuanian University of Health Sciences, Kaunas, Lithuania.

Purpose: Breast cancer is the most frequent female cancer, leading to relapse with distant metastasis of approximately one-third of patients. Cancer is usually considered a genetic disease involving mutations in nuclear DNA. However, genes, coding for mitochondrial proteins or regulatory molecules, are rarely under consideration. Read More

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Investigating the association between rs6983267 polymorphism and susceptibility to gastrointestinal cancers in Iranian population.

Mol Biol Rep 2021 Mar 13. Epub 2021 Mar 13.

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

Genome-wide association studies have revealed that some single nucleotide polymorphisms at 8q24, such as rs6983267, might be effective in susceptibility to various cancers in different populations. Therefore, rs6983267 might be useful as a marker for multiple cancers. In this study, we considered a population, including 478 gastrointestinal cancer cases from the Iranian population, to investigate the association between rs6983267 and susceptibility to gastrointestinal cancers. Read More

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Assessment of Plasmodium falciparum anti-malarial drug resistance markers in pfk13-propeller, pfcrt and pfmdr1 genes in isolates from treatment failure patients in Democratic Republic of Congo, 2018-2019.

Malar J 2021 Mar 11;20(1):144. Epub 2021 Mar 11.

Laboratory of Clinical Microbiology, University of Liège, 4000, Liège, Belgium.

Background: The national policy for malaria treatment of the Democratic Republic of Congo recommends two first-line artemisinin-based combinations for the treatment of uncomplicated malaria: artesunate-amodiaquine and artemether-lumefantrine. This study investigated the presence of markers associated with resistance to the current first-line artemisinin-based combination therapy (ACT) in isolates of Plasmodium falciparum from treatment failure patients in the Democratic Republic of Congo.

Methods: From November 2018 to November 2019, dried blood spots were taken from patients returning to health centres for fever within 28 days after an initial malaria treatment in six sentinel sites of the National Malaria Control Programme across Democratic Republic of Congo. Read More

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E2F1 copy number variations in germline and breast cancer: a retrospective study of 222 Italian women.

Mol Med 2021 03 10;27(1):26. Epub 2021 Mar 10.

Department of Medicine, Unit of Andrology and Reproductive Medicine, University Hospital Padua, Via N. Giustiniani 2, 35128, Padua, Italy.

Background: Breast cancer is the most common neoplasia among women in developed countries. The risk factors of breast cancer can be distinguished in modifiable and unmodifiable factors and, among the latter, genetic factors play a key role. Copy number variations (CNVs) are genetic variants that are classified as rare when present in less than 1% of the healthy population. Read More

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Integrative Morphometric and Molecular Approach to Update the Impact and Distribution of Potato Cyst Nematodes and (Tylenchida: Heteroderidae) in Algeria.

Pathogens 2021 Feb 16;10(2). Epub 2021 Feb 16.

Instituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, Portugal.

Morphological and molecular studies were conducted to characterize the specific identity of 36 isolates of potato cyst nematodes (PCNs) recovered from soil samples collected in several potato producing areas of Algeria. Morphometric data revealed that 44% of isolates contained alone, 28% contained alone and 28% mixtures of the two species. Morphometric values of cysts and second-stage juveniles were generally distributed with slight differences in the expected ranges for both species. Read More

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February 2021

No Association Between or Polymorphisms and Gestational Diabetes Mellitus in South African Women.

Diabetes Metab Syndr Obes 2021 24;14:791-800. Epub 2021 Feb 24.

Biomedical Research and Innovation Platform (BRIP), South African Medical Research Council, Cape Town, 7505, South Africa.

Purpose: Gestational diabetes mellitus (GDM) is a growing public health concern. GDM affects approximately 14% of pregnancies globally, and without effective treatment, is associated with short- and long-term complications in mother and child. Lower serum adiponectin concentrations and aberrant DNA methylation have been reported during GDM. Read More

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February 2021

Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine.

Microbiologyopen 2021 01;10(1):e1169

Department of Veterinary Bacteriology, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.

A novel TaqMan 5-plex real-time PCR using a combination of locked nucleic acid-modified (LNA)- and minor groove binding (MGB)-conjugated DNA probes was developed for identification and differentiation between the four main pathogenic Brachyspira species in swine. B. hyodysenteriae, B. Read More

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January 2021

Noninvasive Fetal Sex Determination by Real-Time PCR and TaqMan Probes.

Rep Biochem Mol Biol 2020 Oct;9(3):315-323

Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Background: Noninvasive fetal sex determination by analyzing Y chromosome-specific sequences is very useful in the management of cases related to sex-linked genetic diseases. The aim of this study was to establish a non-invasive fetal sex determination test using Real-Time PCR and specific probes.

Methods: The study was a prospective observational cohort study conducted from August 2018 to September 2019. Read More

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October 2020