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Relationship between DNA mismatch repair and CRISPR/Cas9-mediated knock-in in the bovine β-casein gene locus.

Anim Biosci 2021 Jun 24. Epub 2021 Jun 24.

Department of Animal Science, Chonnam National University, Gwangju 61186, Korea.

Objective: Efficient gene editing technology is critical for successful knock-in in domestic animals. RAD51 gene plays an important role in strand invasion during homologous recombination (HR) in mammals, and is regulated by CHK1 and CHK2 genes, which are upstream elements of RAD51. In addition, mismatch repair (MMR) system is inextricably linked to HR-related pathways and regulates HR via heteroduplex rejection. Read More

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Blood biochemical parameters and organs development of brown layers fed reduced dietary protein levels in two rearing systems.

Anim Biosci 2021 Jun 24. Epub 2021 Jun 24.

Department of Animal Science, Federal University of Goiás, GO, 74690-900, Brazil.

Objective: An experiment was conducted to evaluate the effect of different levels of crude protein (CP) and two rearing systems (cage and floor), on blood parameters and digestive and reproductive organ development of brown laying hens.

Methods: A total of 400 Hisex Brown laying hens between 30 and 45 weeks of age were distributed in a completely randomized design and a 2×4 factorial arrangement, with main effects including two rearing systems (cage and floor) and levels of CP (140, 150, 160, and 180 g/kg), in a total of eight treatments and five replicates of 10 birds each with initial body weight of 1,877 g (laying hen in cage) and 1,866 g (laying hens in floor). The parameters evaluated were plasma total protein, albumin, uric acid, total cholesterol, relative weights of oviduct, abdominal fat, liver, gizzard, crest and dewlap, length of small intestine and oviduct. Read More

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Adverse childhood experiences and trauma informed care: treating the whole patient with a more complete osteopathic approach.

T Brian Marcoux

J Osteopath Med 2021 Jul 22. Epub 2021 Jul 22.

Division of Child & Adolescent Psychiatry, The Johns Hopkins Hospital, 1800 Orleans St, Baltimore, MD21287-0010, USA.

In 1998, a seminal study identified a strong connection between participants' exposures to adverse childhood experiences (ACEs) and the development of risk factors for serious health conditions later in life. More than two decades later, leaders in both policy and health care professions now appreciate the impact of social determinants of health, including the enormous societal costs incurred by deleterious experiences, and recognize that treating illness begins with prevention in early childhood. The trauma informed care (TIC) model offers a treatment approach that lends consideration to the traumatic experiences that impact a given patient and allows for more complete treatment by their physician. Read More

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Diagnostic accuracy of a novel artificial intelligence system for adenoma detection in daily practice: a prospective non-randomized comparative study.

Endoscopy 2021 Jul 22. Epub 2021 Jul 22.

Department of Gastroenterology and interventional Endoscopy, Krankenhaus Barmherzige Bruder Regensburg, Regensburg, Germany.

Background And Aims: Adenoma detection rate (ADR) varies significantly between endoscopists with up to 26% adenoma miss rate (AMR). Artificial intelligence (AI) systems may improve endoscopic quality and reduce the rate of interval cancer. We evaluated the efficacy of an AI system in real time colonoscopy and its influence on the AMR and the ADR. Read More

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Functional Outcomes and Return to Sport After Cartilage Restoration of the Knee in High-level Athletes.

J Am Acad Orthop Surg 2021 Jul 22. Epub 2021 Jul 22.

From the Department of Orthopaedic Surgery, Kaiser Permanente, Los Angeles, CA (Mehran), the Department of Orthopaedic Surgery, Harbor-UCLA Medical Center, Torrance, CA (Singla), the Department of Orthopaedic Surgery, Mayo Clinic, Rochester, MN (Okoroha), and the Orthopaedic Surgery and Sports Medicine, Gundersen Health System, Onalaska, WI (Mitchell).

Articular cartilage injuries of the knee are being observed with increasing frequency in athletes and have proven to be difficult to treat given the limited regenerative ability of cartilage and the potential for progressive joint degeneration. A wide range of surgical treatments such as microfracture, autologous chondrocyte implantation, and osteochondral autograft and allograft have demonstrated promising results in these high-demand individuals. These procedures permit healing of cartilage defects while decreasing pain and restoring function with patient-reported outcomes demonstrating significant improvement at short-, mid-, and long-term follow-up. Read More

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Relatively Low Rate of Heterotopic Ossification Following Primary Total Knee Arthroplasty: A Systematic Review and Meta-analysis.

J Am Acad Orthop Surg Glob Res Rev 2021 07 22;5(7). Epub 2021 Jul 22.

From the Stavros Niarchos Foundation Complex Joint Reconstruction Center, Hospital for Special Surgery, New York, NY (Dr. Gkiatas, Mr. Xiang, Mr. Windsor, Dr. Malahias, Dr. Tarity, and Dr. Sculco), and the School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece (Mr. Karasavvidis).

Background: Heterotopic ossification (HO) is abnormal growth of ectopic bone and negatively affects the outcomes after total knee arthroplasty (TKA). This systematic review and meta-analysis were performed to characterize the prevalence and severity of HO after primary TKA.

Methods: A systematic review of the literature was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Read More

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Effect of Sustained Interventions from Infancy to Toddlerhood in Children with Cleft Lip and Palate for Preventing Early Childhood Caries.

Caries Res 2021 Jul 22:1-9. Epub 2021 Jul 22.

Department of Pediatric and Preventive Dentistry, Pushpagiri College of Dental Sciences, Thiruvalla, India.

The study aimed to evaluate the effectiveness of sustained interventions in children with cleft lip and palate (CLP) for preventing early childhood caries (ECC). This prospective, nonrandomized interventional cohort study was conducted in infants aged 0-12 months with congenital CLP. Interventions were given to parents/primary caregivers in the form of combined oral health-care measures (sterile wet gauze piece, finger brush, toothbrush, and toothpaste) by a motivational interviewing approach. Read More

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Complexities at the Au/ZrS(001) interface probed by X-ray photoemission spectroscopy.

J Phys Condens Matter 2021 Jul 22. Epub 2021 Jul 22.

Department of Physics and Astronomy and Center for Materials Research and Analysis, University of Nebraska- Lincoln, Jorgensen Hall, 855 North 16th Str., NE 68588-0299, Lincoln, 68588-0299, UNITED STATES.

Interaction between the Au adlayers and ZrS(001) has been examined via X-ray photoemission spectroscopy (XPS). The angle-resolved XPS (ARXPS) measurements reveal that ZrS(001) is disulfide (S) terminated and the Au thickness-dependent XPS indicates that the observed band bending, for low Au coverage, is consistent with formation of a Schottky barrier at the Au/ZrS(001) interface. This band bending, however, appears to be suppressed as the thickness of Au adlayer is increased, indicating varying interfacial interactions at the Au/ZrS(001) interface. Read More

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Synthetic pulmonary perfusion images from 4DCT for functional avoidance using deep learning.

Phys Med Biol 2021 Jul 22. Epub 2021 Jul 22.

Radiation Oncology, Beaumont Health System, Royal Oak, Michigan, UNITED STATES.

Purpose: To develop and evaluate the performance of a deep learning model to generate synthetic pulmonary perfusion images from clinical 4DCT images for patients undergoing radiotherapy for lung cancer.

Methods: A clinical data set of 58 pre- and post-radiotherapy 99mTc-labelled MAA-SPECT perfusion studies (32 patients) each with contemporaneous 4DCT studies was collected. Using the inhale and exhale phases of the 4DCT, a 3D-Residual Network was trained to create synthetic perfusion images utilizing the MAA-SPECT as ground truth. Read More

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Biohybrid generators based on living plants and artificial leaves: Influence of leaf motion and real wind outdoor energy harvesting.

Bioinspir Biomim 2021 Jul 22. Epub 2021 Jul 22.

Center for Micro-Biorobotics, Italian Institute of Technology (IIT), Viale Rinaldo Piaggio 34, Pontedera, 56025, ITALY.

Plants translate wind energy into leaf fluttering and branch motion by reversible tissue deformation. Simultaneously, the plant's outermost structure, i.e. Read More

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The lncRNA OGFRP1/miR-149-5p/IL-6 axis regulates prostate cancer chemoresistance.

Pathol Res Pract 2021 Jun 22;224:153535. Epub 2021 Jun 22.

Department of Laboratory Medicine, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, China. Electronic address:

Background: The long non-coding RNA (lncRNA) OGFRP1 has been found to promote malignancy in prostate cancer (PC) and other cancer types. How this lncRNA functions in the regulation of PC chemoresistance, however, is poorly defined.

Methods: qRT-PCR was employed to measure OGFRP1, miR-149-5p, and IL-6 expression in PC tissues and cells. Read More

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Astrocyte-derived CCL7 promotes microglia-mediated inflammation following traumatic brain injury.

Int Immunopharmacol 2021 Jul 19;99:107975. Epub 2021 Jul 19.

Neurology Department, Jintan Hospital affiliated to Jiangsu University, Changzhou 213200, China. Electronic address:

Microglia are immune cells of the central nervous system that mediate neuroinflammation. It is widely known that microglia-mediated inflammation in the brain contribute to the widespread tissue damage and neurological deficits in traumatic brain injury (TBI). However, the mechanisms responsible for this inflammatory response remain elusive. Read More

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AIRE-overexpressing BMDCs suppress T cells through ICOSL to prevent and attenuate autoimmune diabetes in NOD mice.

Int Immunopharmacol 2021 Jul 19;99:107979. Epub 2021 Jul 19.

Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China. Electronic address:

The strong genetic association between autoimmune regulator (AIRE) and autoimmune diseases indicates its critical role in immune tolerance. AIRE deficiency is thought to promote the development of follicular helper T (T) cells, which are considered to be essential in B cell proliferation. Excessive T cell generation is a key step towards the development of autoimmune diseases, including type 1 diabetes. Read More

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Direct in vivo reprogramming with non-viral sequential targeting nanoparticles promotes cardiac regeneration.

Biomaterials 2021 Jul 15;276:121028. Epub 2021 Jul 15.

Department of Cardiology, Zhongshan Hospital, Fudan University. Shanghai Institute of Cardiovascular Diseases, Shanghai, 20032, China; Institute of Biomedical Sciences, Fudan University, Shanghai, 20032, China. Electronic address:

microRNA-mediated direct cardiac reprogramming, directly converts fibroblasts into induced cardiomyocyte-like cells (iCMs), which holds great promise in cardiac regeneration therapy. However, effective approaches to deliver therapeutic microRNA into cardiac fibroblasts (CFs) to induce in vivo cardiac reprogramming remain to be explored. Herein, a non-viral biomimetic system to directly reprogram CFs for cardiac regeneration after myocardial injury was developed by coating FH peptide-modified neutrophil-mimicking membranes on mesoporous silicon nanoparticles (MSNs) loaded with microRNA1, 133, 208, and 499 (miR Combo). Read More

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Biodegradable and re-usable sponge materials made from chitin for efficient removal of microplastics.

J Hazard Mater 2021 Jul 8;420:126599. Epub 2021 Jul 8.

Institute of Coastal Environmental Pollution Control, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address:

Microplastics have attracted widespread attention due to their detrimental effects on organisms, and their efficient removal poses great challenges, especially those smaller than 3 µm that are more harmful for aquatic biota. Herein, the chitin based sponges with interconnected pores, excellent elasticity and mechanical durability were fabricated and composed with graphene oxide (GO) and oxygen-doped carbon nitride (O-CN). The chitin based sponges could effectively remove different functionalized microplastics (~1 µm) at pH 6-8, including carboxylate-modified polystyrene (PS-COOH), amine-modified polystyrene (PS-NH), and polystyrene (PS). Read More

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Remediation of contaminated dredged harbor sediments by combining hydrodynamic cavitation, hydrocyclone, and persulfate oxidation process.

J Hazard Mater 2021 Jul 6;420:126594. Epub 2021 Jul 6.

Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan. Electronic address:

A pilot-scale hybrid treatment system consisting of hydrodynamic cavitation (HC), hydrocyclone separator (HS), and sodium persulfate (PS), was employed for removing polycyclic aromatic hydrocarbons (PAHs) from dredged harbor sediments. The effectiveness of PAH degradation was studied by varying the inlet pressure (0-2.0 bar), PS dosage (or Σ[PAH] to [PS] mole ratio of 1:1-1:10) at HS inflow velocity of 2. Read More

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Exploring the nexus between agriculture and greenhouse gas emissions in BIMSTEC region: The role of renewable energy and human capital as moderators.

J Environ Manage 2021 Jul 20;297:113316. Epub 2021 Jul 20.

University School of Management Studies, Guru Gobind Singh Indraprastha University, Sector 16C, Dwarka, New Delhi, 110078, India. Electronic address:

Agriculture and the food system emit a considerable amount of Greenhouse Gas (GHG) emissions in the atmosphere. Hence, current researchers, policymakers, and other stakeholders are calling for improving the environmental performance of agriculture. This study utilizes the countries of The Bay of Bengal Initiative for Multi-Sectoral Technical and Economic Cooperation (BIMSTEC) to investigate the effect of agriculture value-added, pesticide use, renewable energy adoption, human capital, and economic growth on greenhouse gas emissions. Read More

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Utilization of solar energy for wastewater treatment: Challenges and progressive research trends.

J Environ Manage 2021 Jul 20;297:113300. Epub 2021 Jul 20.

Center of Research Excellence in Renewable Energy and Power Systems, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; School of Energy Management, Shri Mata Vaishno Devi University, Katra, 182320, Jammu and Kashmir, India. Electronic address:

This article offers a trend of inventions and implementations of photocatalysis process, desalination technologies and solar disinfection techniques adapted particularly for treatment of industrial and domestic wastewater. Photocatalysis treatment of wastewater using solar energy is a promising renewable solution to reduce stresses on global water crisis. Rendering to the United Nation Environment Programme, 1/3 of world population live in water-stressed countries, while by 2025 about 2/3 of world population will face water scarcity. Read More

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Enhancing robustness of activated sludge with Aspergillus tubingensis as a protective backbone structure under high-salinity stress.

J Environ Manage 2021 Jul 19;297:113302. Epub 2021 Jul 19.

State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Bioprocess, Beijing, 100029, People's Republic of China; Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing, 100029, People's Republic of China; Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China. Electronic address:

High salt seriously destroys the stable interactions among key functional species of activated sludge, which in turn limits the performance of high-salinity wastewater biological treatment. In this study, pelletized Aspergillus tubingensis (AT) was used as a protective backbone structure for activated sludge under high-salinity stress, and a superior salt-tolerant AT-based aerobic granular sludge (AT-AGS) was developed. Results showed that the COD and NH-N removal efficiencies of salt-domesticated AT-AGS were 11. Read More

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Identifying optimal virtual water management strategy for Kazakhstan: A factorial ecologically-extended input-output model.

J Environ Manage 2021 Jul 20;297:113303. Epub 2021 Jul 20.

School of Environmental Science and Engineering, Xiamen University of Technology, Xiamen, 361024, China. Electronic address:

Virtual water is an important indicator measuring the amount of water needed from the perspective of consumption, which can help decision makers to identify desired system design and optimal management strategy against water resources shortage. In this study, a novel model named as factorial ecologically-extended input-output model (abbreviated as FEIOM) is developed for virtual water management. FEIOM integrates techniques of input-output model (IOM), ecological network analysis (ENA) and factorial analysis (FA) into a general framework. Read More

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A residual network with attention module for hyperspectral information of recognition to trace the origin of rice.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 15;263:120155. Epub 2021 Jul 15.

School of Automation Engineering, Northeast Electric Power University, Jilin 132012, China. Electronic address:

In this work, a neural network framework for hyperspectral information recognition was proposed, combined with residual block and convolutional block attention module (CBAM) to enhance the detection performance of hyperspectral for tracing the rice quality. Firstly, the hyperspectral image system was used to obtain the hyperspectral information of the rice. Secondly, due to the small data set, the structure of the residual network was designed based on the characteristics of the hyperspectral information to prevent overfitting the model. Read More

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Computer Game Assisted Task Specific Exercises in the Treatment of Motor and Cognitive Function and Quality of Life in Stroke: A Randomized Control Study.

J Stroke Cerebrovasc Dis 2021 Jul 19;30(9):105991. Epub 2021 Jul 19.

Department of Physical and Rehabilitation Medicine, Baskent University Faculty of Medicine, Maresal Fevzi Cakmak Cad. 5. Sok. No: 48, Bahcelievler, Ankara, 06490, Turkey.

Objectives: Computer game assisted task specific exercises (CGATSE) are rehabilitation gaming systems (RGS) used in stroke rehabilitation to facilitate patient performance of high intensity, task based, repetitive exercises aiming to enhance neuroplasticity. CGATSE maybe an appealing option in home based rehabilitation of stroke patients, especially during the COVID-19 pandemic. This study aimed to determine the effects of CGATSE on hemiplegic arm-hand function, cognitive function and quality of life in stroke. Read More

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Inconsistent idealizations and inferentialism about scientific representation.

Peter Tan

Stud Hist Philos Sci 2021 Jul 19;89:11-18. Epub 2021 Jul 19.

Fordham University, Department of Philosophy, Lowenstein 916C, 113 W. 60th St, New York, NY 10023, USA. Electronic address:

Inferentialists about scientific representation hold that an apparatus's representing a target system consists in the apparatus allowing "surrogative inferences" about the target. I argue that a serious problem for inferentialism arises from the fact that many scientific theories and models contain internal inconsistencies. Inferentialism, left unamended, implies that inconsistent scientific models have unlimited representational power, since an inconsistency permits any conclusion to be inferred. Read More

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Threatening Blindness in a Child With Typical Hemolytic Uremic Syndrome.

Pediatr Neurol 2021 Jun 25;122:35-37. Epub 2021 Jun 25.

Department of Paediatric Neurology, APHM La Timone, Marseille, France. Electronic address:

Background: Hemolytic uremic syndrome (HUS) is the most common cause of acute kidney failure in children younger than five years. Central nervous system involvement occurs in 15% of patients, with clinical manifestations including confusion, coma, seizures, stroke, and cortical blindness. Ocular involvement in children with HUS is rare, but retinal and choroidal hemorrhages as well as ischemic retinopathy due to thrombotic microangiopathic lesions have been documented. Read More

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Interfacial structures of particle-stabilized emulsions examined by ultrasonic scattering analysis with a core-shell model.

Ultrasonics 2021 Jul 13;116:106510. Epub 2021 Jul 13.

Department of Macromolecular Science and Engineering, Graduate School of Science & Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address:

Pickering emulsions comprising liquid droplets stabilized by solid microparticles have gained much attention in the field of cosmetics, inks, and drug delivery systems. To ensure that microparticles in Pickering emulsions are localized at the surface of liquid droplets, ultrasonic spectroscopy analysis combined with scattering function theory was conducted in this study. Two specific cases were investigated: (1) silica particles and liquid droplets independently dispersed in liquid and (2) silica particles effectively localized at the surface of the droplets. Read More

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Insourcing and scaling a telemedicine solution in under 2 weeks: Lessons for the digital transformation of health care.

Healthc (Amst) 2021 Jul 19;9(3):100568. Epub 2021 Jul 19.

University of Pennsylvania Health System, Penn Medicine Center for Health Care Innovation, 3400 Civic Center Blvd, 14(th) Floor South Pavilion, Philadelphia, PA, 19104, USA; University of Pennsylvania Health System, Office of the Chief Medical Information Officer, 3400 Civic Center Blvd, Philadelphia, PA, 19104, USA; University of Pennsylvania Health System, Cardiology, 3400 Civic Center Blvd, Philadelphia, PA, 19104, USA. Electronic address:

The Covid-19 pandemic required rapid scale of telemedicine as well as other digital workflows to maintain access to care while reducing infection risk. Both patients and clinicians who hadn't used telemedicine before were suddenly faced with a multi-step setup process to log into a virtual meeting. Unlike in-person examination rooms, locking a virtual meeting room was more error-prone and posed a risk of multiple patients joining the same online session. Read More

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Color discrimination in anomalous trichromacy: Experiment and theory.

Vision Res 2021 Jul 19;188:85-95. Epub 2021 Jul 19.

Department of Psychology, University of California, San Diego, CA, USA.

In anomalous trichromacy, the color signals available from comparing the activities of the two classes of cone sensitive in the medium and long wavelength parts of the spectrum are much reduced from those available in normal trichromacy, and color discrimination thresholds along the red-green axis are correspondingly elevated. Yet there is evidence that suprathreshold color perception is relatively preserved; this has led to the suggestion that anomalous trichromats post-receptorally amplify their impoverished red-green signals. To test this idea, we measured chromatic discrimination from white and from saturated red and green pedestals. Read More

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Abscisic acid and putrescine synergistically regulate the cold tolerance of melon seedlings.

Plant Physiol Biochem 2021 Jul 13;166:1054-1064. Epub 2021 Jul 13.

Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang, 110866, Liaoning, PR China. Electronic address:

Low temperature in early spring severely endangers the growth and development of melon seedlings. Abscisic acid (ABA) and polyamines (PAs) are important signal molecules in plant response to stress. However, the issue of whether they interact to regulate melon cold tolerance remains largely uncharacterized. Read More

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Monomeric C-reactive protein promotes platelets to release mitochondrial DNA in anti-neutrophil cytoplasmic antibody-associated vasculitis.

Mol Immunol 2021 Jul 19;137:228-237. Epub 2021 Jul 19.

Department of Nephrology, Tianjin Medical University General Hospital, Tianjin, 300052, China. Electronic address:

Although high level of circulating C-reactive protein (pCRP) is considered as a biomarker for disease activity, the significance of CRP in the pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) has not been clarified. We once reported in AAV, pentameric CRP (pCRP) could dissociate into monomeric CRP (mCRP) and activate platelets. Recent studies have demonstrated that the activated platelets can release mitochondrial DNA (mtDNA). Read More

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Estradiol correlates with the accumulation of Monocytic Myeloid-Derived Suppressor Cells in Pre-term birth: A possible explanation of immune suppression in pre-term babies.

J Reprod Immunol 2021 Jul 19;147:103350. Epub 2021 Jul 19.

Department of Molecular and Human Genetics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India. Electronic address:

Synergistic interplay of immune endocrine interaction is prerequisite for an effective maternal fetal tolerance. Pre-term birth (PTB) may be a consequence of altered immune-endocrine crosstalk during third trimester resulting in early breakdown of this tolerance. Myeloid derived suppressor cells (MDSCs), a heterogenous population of immature immune cells are increased in pregnant women and healthy newborns, but their role in PTB still remains obscure. Read More

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