Talanta 2021 Mar 12;224:121889. Epub 2020 Nov 12.
Laboratoire de Biochimie et Pharmaco-toxicologie, Centre Hospitalier Lyon-Sud, Hospices Civils de Lyon, F-69495, Pierre Bénite, France; EMR 3738, Ciblage Thérapeutique en Oncologie, Faculté de Médecine Lyon-Sud, F-69921, Oullins, France; Laboratoire de Toxicologie, Faculté de Pharmacie ISPBL, Université Lyon 1, F-69373, Lyon, France. Electronic address:
Nivolumab is a fully human immunoglobulin G4 used for the treatment of several advanced solid cancers as immune checkpoint inhibitors. There are some challenges for the quantification of mAb in plasma because IgG are present intrinsically in complex biologic matrices and this determination must be based on reliable, selective, and accurate analytical methods. This study described two validated methods carried out in two separate laboratories, one developed with a triple quadrupole tandem mass spectrometry (LC-MS/MS) and the other with high resolution mass spectrometry with an orbitrap system (LC-MS/HRMS). Read More