2,205,405 results match your criteria surface proteins

Improved SARS-CoV-2 M inhibitors based on feline antiviral drug GC376: Structural enhancements, increased solubility, and micellar studies.

Eur J Med Chem 2021 May 30;222:113584. Epub 2021 May 30.

Department of Chemistry, University of Alberta, Edmonton AB, T6G 2G2, Canada. Electronic address:

Replication of SARS-CoV-2, the coronavirus causing COVID-19, requires a main protease (M) to cleave viral proteins. Consequently, M is a target for antiviral agents. We and others previously demonstrated that GC376, a bisulfite prodrug with efficacy as an anti-coronaviral agent in animals, is an effective inhibitor of M in SARS-CoV-2. Read More

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High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.

Transl Oncol 2021 Jun 9;14(8):101146. Epub 2021 Jun 9.

Department of Otolaryngology Head & Neck Surgery, The Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi-Zao-Ju Road, Shanghai 200011, China; Ear Institute, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China. Electronic address:

Head and neck paragangliomas (HNPGLs) are rare neoplasms that represent difficult treatment paradigms in neurotology. Germline mutations in genes encoding succinate dehydrogenase (SDH) are the cause of nearly all familial HNPGLs. However, the molecular mechanisms underlying tumorigenesis remain unclear. Read More

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PLAC1 affects cell to cell communication by interacting with the desmosome complex.

Placenta 2021 Jun 6;110:39-45. Epub 2021 Jun 6.

Laboratory of Genetics and Genomics, National Institute on Aging, Baltimore, MD, 21224, USA. Electronic address:

Introduction: X-linked PLAC1 is highly expressed in placenta during embryogenesis, and when ablated in mice, causes aberrant placental cell layer organization. It is also highly expressed in many types of cancer cell-lines. Although it has been shown that it promotes AKT phosphorylation in cancer cells, the exact mechanism by which it influences placental layer differentiation is unclear. Read More

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Compctutational identification of putative common genomic drug and vaccine targets in Mycoplasma genitalium.

Genomics 2021 Jun 9. Epub 2021 Jun 9.

Department of Immunology, Microbiology and Parasitology, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil. Electronic address:

Mycoplasma genitalium is an obligate intracellular bacterium that is responsible for several sexually transmitted infections, including non-gonococcal urethritis in men and several inflammatory reproductive tract syndromes in women. Here, we applied subtractive genomics and reverse vaccinology approaches for in silico prediction of potential vaccine and drug targets against five strains of M. genitalium. Read More

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Positive Selection as a Key Player for SARS-CoV-2 Pathogenicity: Insights into ORF1ab, S and E genes.

Virus Res 2021 Jun 9:198472. Epub 2021 Jun 9.

Center for Informatics Sciences (CIS), Nile University, Giza, Egypt.

The human β-coronavirus SARS-CoV-2 epidemic started in late December 2019 in Wuhan, China. It causes Covid-19 disease which has become pandemic. Each of the five-known human β-coronaviruses has four major structural proteins (E, M, N and S) and 16 non-structural proteins encoded by ORF1a and ORF1b together (ORF1ab) that are involved in virus pathogenicity and infectivity. Read More

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The murburn precepts for aerobic respiration and redox homeostasis.

Prog Biophys Mol Biol 2021 Jun 9. Epub 2021 Jun 9.

Institute of Chemical Kinetics and Combustion Russian Academy of Sciences, St. Institutskaya 3, 630090, Novosibirsk, Russia. Electronic address:

Murburn concept is a new perspective to metabolism which posits that certain redox enzymes/proteins mediate catalysis outside their active site, via diffusible reactive oxygen species (DROS, usually deemed as toxic wastes). We have recently questioned the proton-centric chemiosmotic rotary ATP synthesis (CRAS) explanation for mitochondrial oxidative phosphorylation (mOxPhos) and proposed an oxygen-centric murburn model in lieu. Herein, the chemical equations and thermodynamic foundations for this new model of mOxPhos are detailed. Read More

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Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus.

J Immunol Methods 2021 Jun 9:113084. Epub 2021 Jun 9.

Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. Electronic address:

Bovine Leukemia Virus (BLV) is an oncogenic virus which is the etiological agent of a neoplastic disease in infected cattle called enzootic bovine leukemia (EBL). The most common and sensitive diagnostic methods for EBL like enzyme-linked immunosorbent assay (ELISA) is time-consuming and requires manual handling which makes it unsuitable as an on-farm diagnostic test. Hence, there is a need for an alternative test with rapid detection and reduced manual labour. Read More

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The extracellular matrix proteins type I collagen, type III collagen, fibronectin, and laminin 421 stimulate migration of cancer cells.

FASEB J 2021 Jul;35(7):e21692

Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.

For metastasis formation, individual cells from a primary tumor must migrate toward other tissues. The aim of this study was to determine if mesenchymal stromal cells (MSCs) from human bone marrow are able to emit signals that induce this migratory activity in cancer cells. We separated the supernatant of MSCs derived from human bone marrow by size-exclusion and ion-exchange chromatography and have subsequently studied the migratory behavior of the prostate cancer cell line PC3 and the breast cancer cell line MDA-MB-231 toward the respective fractions in a transwell migration assay. Read More

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Protein Supercomplex Recording in Living Cells Via Position-Specific Fluorescence Lifetime Sensors.

Methods Mol Biol 2021 ;2275:301-314

Department of Biology, Institute of Molecular Cell Biology, University of Münster, Münster, Germany.

Our group has previously established a strategy utilizing fluorescence lifetime probes to image membrane protein supercomplex (SC) formation in situ. We showed that a probe at the interface between individual mitochondrial respiratory complexes exhibits a decreased fluorescence lifetime when a supercomplex is formed. This is caused by electrostatic interactions with the adjacent proteins. Read More

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January 2021

Species-Specific Urothelial Toxicity with an anti-HIV Non-Catalytic Site Integrase Inhibitor (NCINI) is Related to Unusual pH-Dependent Physicochemical Changes.

Toxicol Sci 2021 Jun 12. Epub 2021 Jun 12.

Nonclinical Safety & Pathobiology, Gilead Sciences, Inc, Foster City, USA.

GS-9695 and GS-9822 are next generation Non-Catalytic Site Integrase Inhibitors (NCINIs) with significantly improved potency against HIV compared with previous drugs such as BI-224436. Development stopped due to vacuolation of the bladder urothelium seen in cynomolgus monkey but not in rat; this lesion was absent in equivalent preclinical studies with BI-224436 (tested in dog and rat). Lesions were unlikely to be attributable to target since NCINIs specifically target viral integrase protein and no mammalian homologue is known. Read More

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MoSep3 and MoExo70 are needed for MoCK2 ring assembly essential for appressorium function in the rice blast fungus, Magnaporthe oryzae.

Mol Plant Pathol 2021 Jun 11. Epub 2021 Jun 11.

State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Polar growth during appressorium formation is vital for the penetration peg formation in the rice blast fungus, Magnaporthe oryzae. Previous research has shown that the Sln1-septin-exocyst complex, localized at the base of the appressorium in contact with the leaf surface, forms a ring structure that influences growth polarity and affects penetration peg formation, and is necessary for pathogenicity. Our previous research showed CK2 proteins assemble another ring structure positioned perpendicular to the Sln1-septin-exocyst complex. Read More

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Environmental dimensions of the protein corona.

Nat Nanotechnol 2021 Jun 11;16(6):617-629. Epub 2021 Jun 11.

School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.

The adsorption of biomolecules to the surface of engineered nanomaterials, known as corona formation, defines their biological identity by altering their surface properties and transforming the physical, chemical and biological characteristics of the particles. In the first decade since the term protein corona was coined, studies have focused primarily on biomedical applications and human toxicity. The relevance of the environmental dimensions of the protein corona is still emerging. Read More

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Assessment of a complete and classified platelet proteome from genome-wide transcripts of human platelets and megakaryocytes covering platelet functions.

Sci Rep 2021 Jun 11;11(1):12358. Epub 2021 Jun 11.

Department of Biochemistry, CARIM, Maastricht University, P.O. Box 616, 6200 MD, Maastricht, The Netherlands.

Novel platelet and megakaryocyte transcriptome analysis allows prediction of the full or theoretical proteome of a representative human platelet. Here, we integrated the established platelet proteomes from six cohorts of healthy subjects, encompassing 5.2 k proteins, with two novel genome-wide transcriptomes (57. Read More

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Optoregulated force application to cellular receptors using molecular motors.

Nat Commun 2021 Jun 11;12(1):3580. Epub 2021 Jun 11.

INM - Leibniz Institute for New Materials, Saarbrücken, Germany.

Progress in our understanding of mechanotransduction events requires noninvasive methods for the manipulation of forces at molecular scale in physiological environments. Inspired by cellular mechanisms for force application (i.e. Read More

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K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR.

Cell Death Dis 2021 Jun 11;12(6):608. Epub 2021 Jun 11.

Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Dual specificity tyrosine phosphorylation regulated kinase 1A, DYRK1A, functions in multiple cellular pathways, including signaling, endocytosis, synaptic transmission, and transcription. Alterations in dosage of DYRK1A leads to defects in neurogenesis, cell growth, and differentiation, and may increase the risk of certain cancers. DYRK1A localizes to a number of subcellular structures including vesicles where it is known to phosphorylate a number of proteins and regulate vesicle biology. Read More

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Assessment of immunogenicity and safety across two manufacturing lots of a 3-antigen hepatitis B vaccine, Sci-B-Vac®, compared with Engerix-B® in healthy Asian adults: A phase 3 randomized clinical trial.

Vaccine 2021 Jun 8. Epub 2021 Jun 8.

VBI Vaccines Inc., Cambridge, MA, United States.

Background: Sci-B-Vac®, a 3-antigen hepatitis B vaccine (3A-HBV), contains all three recombinant hepatitis B virus (HBV) envelope proteins (S, pre-S1, and pre-S2). In 2005, 3A-HBV manufacturing transferred facilities (A to B), where it continues to be manufactured.

Methods: This phase 3, single-blind, randomized study, conducted at one site in Vietnam, compared efficacy and safety among two 3A-HBV lots, lot A and lot B, and a single-antigen hepatitis B vaccine (1A-HBV), Engerix-B®. Read More

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Isolation methods commonly used to study the liposomal protein corona suffer from contamination issues.

Acta Biomater 2021 Jun 8. Epub 2021 Jun 8.

DTU Health Tech, Department of Health Technology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. Electronic address:

The liposomal protein corona has been the focus of numerous studies, but there is still no consensus regarding its extent and composition. Rather, the literature is full of conflicting reports on the matter. To elucidate whether there could be a methodological explanation for this, we here scrutinize the efficiency of three commonly used liposome isolation methods at isolating stealth liposomes from human plasma. Read More

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Total saponins from Tupistra chinensis Baker inhibits growth of human gastric cancer cells in vitro and in vivo.

J Ethnopharmacol 2021 Jun 8:114323. Epub 2021 Jun 8.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; Guangdong Engineering Research Center for Lead Compounds & Drug Discovery, Guangzhou 510006, China. Electronic address:

Ethnopharmacological Relevance: Tupistra chinensis Baker (syn. Rohdea chinensis), a antitumor folk herb mainly distributed in China, its rhizome has been historically used to treat gastric cancer. Studies showed that the steroidal saponins were the main bioactive components in the rhizome of T. Read More

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A glimpse at the glycoRNA world.

Matthew D Disney

Cell 2021 Jun;184(12):3080-3081

The Scripps Research Institute, Department of Chemistry, Jupiter, FL 33458, USA. Electronic address:

In the past several decades, there has been an increased appreciation of RNA modifications and their biological functions. In this issue of Cell, Flynn et al. describe the discovery of glycoRNAs present on the surface of cells. Read More

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Structural Changes beyond the EF-Hand Contribute to Apparent Calcium Binding Affinities: Insights from Parvalbumins.

J Phys Chem B 2021 Jun 11. Epub 2021 Jun 11.

Stritch School of Medicine, Maywood, Illinois 60153, United States.

Members of the parvalbumin (PV) family of calcium (Ca) binding proteins (CBPs) share a relatively high level of sequence similarity. However, their Ca affinities and selectivities against competing ions like Mg can widely vary. We conducted molecular dynamics simulations of several α-parvalbumin (αPV) constructs with micromolar to nanomolar Ca affinities to identify structural and dynamic features that contribute to their binding of ions. Read More

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Proximity Proteomics Has Potential for Extracellular Vesicle Identification.

J Proteome Res 2021 Jun 11. Epub 2021 Jun 11.

Department of Biochemistry, Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan.

Extracellular vesicles (EVs) are biomarkers and mediators of intercellular communication. In biological samples, EVs are secreted by various types of cells. The proteomic identification of proteins expressed in EVs has potential to contribute to research and clinical applications, particularly for cancer. Read More

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Improved Protocol to Tackle the pH Effects on Membrane-Inserting Peptides.

J Chem Theory Comput 2021 Jun 11. Epub 2021 Jun 11.

Departamento de Química e Bioquímica, Faculdade de Ciências, BioISI: Biosystems and Integrative Sciences Institute, Universidade de Lisboa, 1749-016 Lisboa, Portugal.

Many important biological pathways rely on membrane-interacting peptides or proteins, which can alter the biophysical properties of the cell membrane by simply adsorbing to its surface to undergo a full insertion process. To study these phenomena with atomistic detail, model peptides have been used to refine the current computational methodologies. Improvements have been made with force-field parameters, enhanced sampling techniques to obtain faster sampling, and the addition of chemical-physical properties, such as pH, whose influence dramatically increases at the water/membrane interface. Read More

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Testicular torsion-detorsion causes dysfunction of mitochondrial oxidative phosphorylation.

Andrology 2021 Jun 11. Epub 2021 Jun 11.

Integrative Research Centre for Critical Care, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.

Background: Semen quality impairment is a serious consequence of testicular torsion-detorsion (TD). Adequate germ-cell mitochondrial oxidative phosphorylation (OXPHOS) plays a crucial role in male fertility. Changes in cellular OXPHOS in testicular tissues after testicular TD remain unclear. Read More

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Survival motor neuron deficiency slows myoblast fusion through reduced myomaker and myomixer expression.

J Cachexia Sarcopenia Muscle 2021 Jun 11. Epub 2021 Jun 11.

Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, F. Edward Hébert School of Medicine, Bethesda, MD, USA.

Background: Spinal muscular atrophy is an inherited neurodegenerative disease caused by insufficient levels of the survival motor neuron (SMN) protein. Recently approved treatments aimed at increasing SMN protein levels have dramatically improved patient survival and have altered the disease landscape. While restoring SMN levels slows motor neuron loss, many patients continue to have smaller muscles and do not achieve normal motor milestones. Read More

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Minor pilins are involved in motility and natural competence of the cyanobacterium Synechocystis sp. PCC 6803.

Mol Microbiol 2021 Jun 11. Epub 2021 Jun 11.

Molecular Genetics, Institute of Biology III, University of Freiburg, Freiburg, Germany.

Cyanobacteria synthesize type IV pili, which are known to be essential for motility, adhesion and natural competence. They consist of long flexible fibres that are primarily composed of the major pilin PilA1 in Synechocystis sp. PCC 6803. Read More

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Cell-penetrating Mitochondria-targeting Ligands for Universal Delivery of Small Molecules, Proteins and Nanomaterials.

Chemistry 2021 Jun 11. Epub 2021 Jun 11.

National University of Singapore, Department of Chemistry, 3 Science Dr., 117543, Singapore, SINGAPORE.

Mitochondria are key organelles that perform vital cellular functions such as cell survival and death. Targeted delivery of different types of cargos to mitochondria is a well-established strategy to study mitochondrial biology and diseases. Of various existing mitochondria-transporting vehicles, most of them suffered from poor cytosolic entry, low delivery efficiency, limited cargo types, and cumbersome preparation protocols, and none was known to be universally applicable for mitochondrial delivery of different types of cargos (small molecules, proteins and nanomaterials). Read More

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Glass Slide-Printed Protein Arrays as a Platform to Discover Serodiagnostic Antigens Against Bacterial Infections.

Methods Mol Biol 2021 ;2344:151-161

Departamento de Bioquímica y Biología Molecular, Edificio "Severo Ochoa" Planta Baja, Campus de Rabanales, Universidad de Córdoba, Córdoba, Spain.

Infectious diseases represent a major cause of morbidity and mortality worldwide. Early detection of infections is capital for managing life-threatening cases. So far, traditional diagnostic methods such as microbiological cultures are slow and, sometimes, inaccurate. Read More

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January 2021

CXCL2-CXCR2 axis mediates αV integrin-dependent peritoneal metastasis of colon cancer cells.

Clin Exp Metastasis 2021 Jun 11. Epub 2021 Jun 11.

Section of Surgery, Department of Clinical Sciences, Malmö, Skåne University Hospital, Lund University, 20502, Malmö, Sweden.

Peritoneal metastasis is an insidious aspect of colorectal cancer. The aim of the present study was to define mechanisms regulating colon cancer cell adhesion and spread to peritoneal wounds after abdominal surgery. Mice was laparotomized and injected intraperitoneally with CT-26 colon carcinoma cells and metastatic noduli in the peritoneal cavity was quantified after treatment with a CXCR2 antagonist or integrin-αV-antibody. Read More

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How Influenza's Spike Motor Works.

Phys Rev Lett 2021 May;126(21):218101

Institut Charles Sadron UPR22-CNRS, 67034 Strasbourg, France and Institute Theory of Polymers, Leibniz-Institute of Polymer Research, D-01069 Dresden, Germany.

While often believed to be a passive agent that merely exploits its host's metabolism, the influenza virus has recently been shown to actively move across glycan-coated surfaces. This form of enzymatically driven surface motility is currently not well understood and has been loosely linked to burnt-bridge Brownian ratchet mechanisms. Starting from known properties of influenza's spike proteins, we develop a physical model that quantitatively describes the observed motility. Read More

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Advances in exosome therapies in ophthalmology-From bench to clinical trial.

Acta Ophthalmol 2021 Jun 11. Epub 2021 Jun 11.

Faculty of Life Sciences & Medicine, King's College London, London, UK.

During the last decade, the fields of advanced and personalized therapeutics have been constantly evolving, utilizing novel techniques such as gene editing and RNA therapeutic approaches. However, the method of delivery and tissue specificity remain the main hurdles of these approaches. Exosomes are natural carriers of functional small RNAs and proteins, representing an area of increasing interest in the field of drug delivery. Read More

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