21,362 results match your criteria supplemented studies

Biomechanical analysis of lumbar interbody fusion supplemented with various posterior stabilization systems.

Eur Spine J 2021 May 4. Epub 2021 May 4.

Interdisciplinary Division of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China.

Purpose: Biomechanical comparison between rigid and non-rigid posterior stabilization systems following lumbar interbody fusion has been conducted in several studies. However, most of these previous studies mainly focused on investigating biomechanics of adjacent spinal segments or spine stability. The objective of the present study was to compare biomechanical responses of the fusion devices when using different posterior instrumentations. Read More

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Randomized, double-blind, placebo-controlled clinical trial on the usefulness of probiotic Lactobacillus reuteri in bismuth-containing quadruple eradication therapy for infection with Helicobacter pylori.

Rev Esp Enferm Dig 2021 May 5. Epub 2021 May 5.

Aparato Digestivo, Hospital Virgen Macarena, España.

Introduction: The primary goal of this study was to compare gastrointestinal symptom reduction in patients on bismuth-containing quadruple eradication therapy supplemented with Lactobacillus reuteri strains (DSM 17938 and ATCC PTA 6475) or placebo.

Materials And Methods: This was randomized, double-blind, parallel-arm, placebo-controlled clinical trial. Patients received first-line an eradication regimen based on bismuth subcitrate potassium, metronidazole, tetracycline hydrochloride (three-in-one capsules), and omeprazole 40 mg twice a day for ten days, plus a probiotic or placebo tablet for 30 days. Read More

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Quality and Shelf Life of Fresh Meat from Iberian Pigs as Affected by a New Form of Presentation of Oleic Acid and an Organic-Acid Mix in the Diet.

Foods 2021 Apr 30;10(5). Epub 2021 Apr 30.

Estación Tecnológica de la Carne, Instituto Tecnológico Agrario de Castilla y León (ITACyL), Calle Filiberto Villalobos, 5, 37770 Guijuelo, Spain.

The objective of the study was to evaluate the inclusion of a novel form of oleic acid and an organic-acid mix in the diet of Iberian pigs, and their effect on the quality and shelf-life of the pig meat. 200 castrated male Iberian pigs were randomly assigned to four groups. Diets included different fat sources: pig fat (G1), solid oleic acid (G2), oleic-high sunflower oil with solid oleic (G3); a diet of G3 supplemented with organic-acid mix (G4). Read More

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Quantifying the impact of cell culture media on CHO cell growth and protein production.

Biotechnol Adv 2021 May 1:107761. Epub 2021 May 1.

Department of Process Engineering and Applied Science, Dalhousie University, 1360 Barrington St., PO Box 15000, Halifax, NS B3H 4R2, Canada. Electronic address:

In recombinant protein production, cell culture media development and optimization is typically seen as a useful strategy to increase titer and cell density, reduce by-products, as well as improve product quality (with cell density and titer often serving as the primary reported outcome of media studies). However, despite the large number of media optimization studies, there have been few attempts to comprehensively assess the overall effectiveness of media additives. The aim of this review is therefore both to document published media optimization studies over the last twenty years (in the context of Chinese hamster ovary cell recombinant production) and quantitatively estimate the impact of this media optimization on cell culture performance. Read More

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Bioactive Stigmastadienone from Isodon rugosus as potential anticholinesterase, α-glucosidase and COX/LOX inhibitor: In-vitro and molecular docking studies.

Steroids 2021 May 1:108857. Epub 2021 May 1.

Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia. Electronic address:

Natural product is a well-known source of bioactive compounds. Herein, a steroidal compound stigmasta-7,22-diene-3-one (stigmastadienone) has been isolated from Isodon rugosus. The potency of isolated compound has been tested for several in-vitro targets. Read More

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A novel strategy for isolation of mice bone marrow endothelial cells (BMECs).

Stem Cell Res Ther 2021 May 3;12(1):267. Epub 2021 May 3.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, 221002, China.

Background: In the bone marrow microenvironment (BM), endothelial cells are individual cells that form part of the sinusoidal blood vessels called the "bone marrow endothelial-vascular niche." They account for less than 2% of the bone marrow cells. They play essential functions by generating growth and inhibitory factors that promote the hematopoietic stem cells (HSCs) regulation. Read More

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Implementation outcomes of HIV self-testing in low- and middle- income countries: A scoping review.

PLoS One 2021 3;16(5):e0250434. Epub 2021 May 3.

Department of Medical Social Sciences, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.

Introduction: HIV self-testing (HIV-ST) is an effective means of improving HIV testing rates. Low- and middle-income countries (LMIC) are taking steps to include HIV-ST into their national HIV/AIDS programs but very few reviews have focused on implementation in LMIC. We performed a scoping review to describe and synthesize existing literature on implementation outcomes of HIV-ST in LMIC. Read More

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Micronutrient status in bariatric surgery patients receiving postoperative supplementation per guidelines: Insights from a systematic review and meta-analysis of longitudinal studies.

Obes Rev 2021 May 2:e13249. Epub 2021 May 2.

Center for Obesity and Metabolic Diseases, Korea University Anam Hospital, Seoul, South Korea.

The micronutrient status and optimal monitoring schedule after bariatric surgery have not been sufficiently assessed. This systematic review and meta-analysis investigated the longitudinal changes in micronutrient status after bariatric surgery. PubMed, EMBASE, and Cochrane Library were searched for articles that measured preoperative and postoperative serum micronutrient levels in patients undergoing Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG). Read More

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PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

Exp Ther Med 2021 Jun 14;21(6):613. Epub 2021 Apr 14.

Department of General Surgery, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China.

Endoplasmic reticulum stress (ERS)-induced apoptosis serves a crucial role in the pathogenesis of myocardial ischemia/reperfusion injury (MIRI). Previous studies have confirmed that pleckstrin homology-like domain family A member 3 (PHLDA3) is an important mediator in ERS-associated apoptosis. The aim of the current study focused on whether PHLDA3 served protective effects on hypoxia/reoxygenation (H/R)-injured cardiomyocytes by inhibiting ERS-induced apoptosis. Read More

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Virtual Reality Augments Movement during Physical Therapy: A Pragmatic Randomized Trial.

Am J Phys Med Rehabil 2021 Apr 30. Epub 2021 Apr 30.

Division of Pediatric Anesthesia; Department of Anesthesiology, Perioperative, and Pain Medicine; Stanford School of Medicine, Stanford, USA. ATI Physical Therapy, Palo Alto, CA.

Objective: Virtual reality (VR) facilitates physical therapy (PT) via improved engagement. While shown to benefit specific patient populations, such as stroke patients, it is less established in otherwise healthy adults and children receiving outpatient PT. The primary objective was to compare total PT-guided Movement supplemented with VR (PTMVR) to PT guided Movement alone, without VR (PTM). Read More

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Impact of an Infant Formula Containing a Novel Fat Blend (Cow's Milk Fat, Fish and Vegetable Oil) and Prebiotics on Stool Fatty Acid Soaps and Erythrocyte Fatty Acid Profiles in Full-Term Healthy Newborns.

Ann Nutr Metab 2021 Apr 30:1-8. Epub 2021 Apr 30.

Department of Nutrition and Food Sciences, Nutritional Physiology, University of Bonn, Bonn, Germany.

Introduction: Recently, new commercial infant formulas have been composed considering novel fat blends and oligosaccharides to better resemble the fatty acid (FA) composition and stereospecific distribution (e.g., increased amount of ß-palmitate) as well as probiotics content of human breast milk. Read More

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Changes in lipoprotein particle subclasses, standard lipids, and apolipoproteins after supplementation with n-3 or n-6 PUFAs in abdominal obesity: A randomized double-blind crossover study.

Clin Nutr 2021 Apr 3;40(5):2556-2575. Epub 2021 Apr 3.

Department of Heart Disease, Haukeland University Hospital, 5021 Bergen, Norway; Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, 5020 Bergen, Norway. Electronic address:

Background & Aims: Marine-derived omega-3 (n-3) polyunsaturated fatty acids (PUFAs), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), lower circulating levels of triacylglycerols (TAGs), and the plant-derived omega-6 (n-6) PUFA linoleic acid (LA) may reduce cholesterol levels. Clinical studies on effects of these dietary or supplemental PUFAs on other blood fat fractions are few and have shown conflicting results. This study aimed to determine effects of high-dose supplemental n-3 (EPA + DHA) and n-6 (LA) PUFAs from high-quality oils on circulating lipoprotein subfractions and standard lipids (primary outcomes), as well as apolipoproteins, fatty acids, and glycemic control (secondary outcomes), in females and males with abdominal obesity. Read More

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Sleep contributes to preference for novel food odours in Drosophila melanogaster.

Sci Rep 2021 Apr 30;11(1):9395. Epub 2021 Apr 30.

Research Institute of Green Science and Technology, Shizuoka University, Oya 836, Shizuoka, 422-8529, Japan.

The importance of sleep in maintaining cognitive functions such as learning and memory has been reported in both vertebrates and invertebrates. Previous studies demonstrated that sleep deprivation impaired the olfactory memory retention of fruit flies as described in the classical conditioning paradigm. Here, we show that sleep deprivation leads to a preference for the odours of the rearing environment in Drosophila melanogaster. Read More

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VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes.

Stem Cells Int 2021 10;2021:8888575. Epub 2021 Apr 10.

Department of Cardiothoracic Surgery, Heart Center of the University of Cologne, Cologne, Germany.

Myocardial hypertrophy is present in many heart diseases, representing a strong predictor of adverse cardiovascular outcomes. Regarding therapeutic intervention, mesenchymal stem cells (MSCs) have been suggested to significantly reduce cardiac hypertrophy and progression to heart failure. Preconditioning of MSCs was previously demonstrated to highly improve their paracrine activity resulting in modulation of immune responses and the progression of diseases. Read More

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Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats.

J Poult Sci 2021 Apr;58(2):119-130

Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia.

An experiment in broilers was conducted to investigate the effect of olive () leaves and marigold () petal extract supplementation on oxidative stress, characteristics of intestinal contents, and on the morphology of the small intestine. Oxidative stress was induced by a n-3 polyunsaturated fatty acids rich diet. 1-day-old male broiler chickens, Ross 308, were housed in a deep litter system. Read More

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Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties.

Microorganisms 2021 Apr 13;9(4). Epub 2021 Apr 13.

Laboratory of Plant Ecophysiology, Faculty of Natural Resources, Campus Luis Rivas del Canto, Catholic University of Temuco, Rudecindo Ortega #03694, 4780000 Temuco, Chile.

Five macrolichens of different thallus morphology from Antarctica (King George Island) were used for this ecophysiological study. The effect of thallus desiccation on primary photosynthetic processes was examined. We investigated the lichens' responses to the relative water content (RWC) in their thalli during the transition from a wet (RWC of 100%) to a dry state (RWC of 0%). Read More

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Increased Elasticity Modulus of Polymeric Materials Is a Source of Surface Alterations in the Human Body.

J Funct Biomater 2021 Apr 16;12(2). Epub 2021 Apr 16.

Department of Surgery, Klinikum Guetersloh, Reckenberger Strasse 19, 33332 Guetersloh, Germany.

The introduction of alloplastic materials (meshes) in hernia surgery has improved patient outcome by a radical reduction of hernia recurrence rate, but discussion about the biocompatibility of these implanted materials continues since observations of surface alterations of polypropylene and other alloplastic materials were published. This study intends to investigate if additives supplemented to alloplastic mesh materials merge into the solution and become analyzable. Four polypropylene and one polyester alloplastic material were incubated in different media for three weeks: distilled water, saline solution, urea solution, formalin, and hydrogen peroxide. Read More

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Dietary Oleocanthal Supplementation Prevents Inflammation and Oxidative Stress in Collagen-Induced Arthritis in Mice.

Antioxidants (Basel) 2021 Apr 23;10(5). Epub 2021 Apr 23.

Department of Pharmacology, School of Pharmacy, Universidad de Sevilla, 41012 Seville, Spain.

Oleocanthal (OLE), a characteristic and exclusive secoiridoid of family, is mainly found in extra virgin olive oil (EVOO). Previous studies have reported its antioxidant, anti-inflammatory, antimicrobial, anticancer and neuroprotective effects. Since the pathogenesis of rheumatoid arthritis (RA) involves inflammatory and oxidative components, this study was designed to evaluate the preventive role of dietary OLE-supplemented effects in collagen-induced arthritis (CIA) murine model. Read More

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Postnatal Iron Supplementation with Ferrous Sulfate vs. Ferrous Bis-Glycinate Chelate: Effects on Iron Metabolism, Growth, and Central Nervous System Development in Sprague Dawley Rat Pups.

Nutrients 2021 Apr 22;13(5). Epub 2021 Apr 22.

Department of Nutrition, University of California, Davis, CA 95616, USA.

Iron-fortified formulas and iron drops (both usually ferrous sulfate, FS) prevent early life iron deficiency, but may delay growth and adversely affect neurodevelopment by providing excess iron. We used a rat pup model to investigate iron status, growth, and development outcomes following daily iron supplementation (10 mg iron/kg body weight, representative of iron-fortified formula levels) with FS or an alternative, bioavailable form of iron, ferrous bis-glycinate chelate (FC). On postnatal day (PD) 2, sex-matched rat litters ( = 3 litters, 10 pups each) were randomly assigned to receive FS, FC, or vehicle control until PD 14. Read More

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A Cell-Based Approach to Dental Pulp Regeneration Using Mesenchymal Stem Cells: A Scoping Review.

Sahng G Kim

Int J Mol Sci 2021 Apr 22;22(9). Epub 2021 Apr 22.

Division of Endodontics, Columbia University College of Dental Medicine, New York, NY 10032, USA.

Despite the recent explosion of investigations on dental pulp regeneration using various tissue engineering strategies, the translation of the findings from such studies into therapeutic applications has not been properly achieved. The purpose of this scoping review was to systematically review the efficacy of mesenchymal stem cell transplantation for dental pulp regeneration. A literature search was conducted using five electronic databases from their inception to January 2021 and supplemented by hand searches. Read More

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In Vitro Investigation of Vascular Permeability in Endothelial Cells from Human Artery, Vein and Lung Microvessels at Steady-State and Anaphylactic Conditions.

Biomedicines 2021 Apr 19;9(4). Epub 2021 Apr 19.

Department of Allergy and Immunology, IIS-Fundación Jiménez Díaz, UAM, 28040 Madrid, Spain.

Human anaphylactic reactions largely involve an increase in vascular permeability, which is mainly controlled by endothelial cells (ECs). Due to the acute and serious nature of human anaphylaxis, in vivo studies of blood vessels must be replaced or supplemented with in vitro models. Therefore, we used a macromolecular tracer assay (MMTA) to investigate the EC permeability of three phenotypes of human ECs: artery (HAECs), vein (HSVECs) and microvessels from lung (HMLECs). Read More

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Relation between Vitamin D and COVID-19 in Aged People: A Systematic Review.

Nutrients 2021 Apr 17;13(4). Epub 2021 Apr 17.

Department of Geriatrics, General Hospital of Valenciennes, 59300 Valenciennes, France.

Background: Vitamin D has diverse and extensive effects on the immune system, including activating innate immunity and reducing the overactive adaptive immune response. A systematic review was performed to identify and synthesize the best available evidence on the association between vitamin D level and risk of COVID-19, adverse outcomes and possible benefits of supplementation in aged 60 years or over.

Methods: A literature search was performed in PubMed© and Scopus© for all publications from inception published before 15 March 2021. Read More

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The Leloir Cycle in Glioblastoma: Galactose Scavenging and Metabolic Remodeling.

Cancers (Basel) 2021 Apr 10;13(8). Epub 2021 Apr 10.

Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX 77030, USA.

Background: Glioblastoma (GBM) can use metabolic fuels other than glucose (Glc). The ability of GBM to use galactose (Gal) as a fuel via the Leloir pathway is investigated.

Methods: Gene transcript data were accessed to determine the association between expression of genes of the Leloir pathway and patient outcomes. Read More

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Gut Microbiota Dynamics, Growth Performance, and Gut Morphology in Broiler Chickens Fed Diets Varying in Energy Density with or without Bacitracin Methylene Disalicylate (BMD).

Microorganisms 2021 Apr 9;9(4). Epub 2021 Apr 9.

Department of Animal Science and Aquaculture, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, Canada.

High-energy-density diet could increase body weight at the expense of the intestinal health of the animals. In order to optimize production without negatively influencing the gut health of chickens, dietary supplementation with bacitracin methylene disalicylate (BMD) is a common feeding strategy adopted to enhance production performance and intestinal health. Studies have suggested that BMD could improve chicken growth performance and gut health through modulation of the gut microbiota. Read More

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Treating Diabetic Foot Osteomyelitis: A Practical State-of-the-Art Update.

Medicina (Kaunas) 2021 Apr 1;57(4). Epub 2021 Apr 1.

Department of Orthopedic Surgery, Balgrist University Hospital, University of Zurich, 8008 Zurich, Switzerland.

Diabetic foot osteomyelitis (DFO) can be difficult to treat and securing optimal clinical outcomes requires a multidisciplinary approach involving a wide variety of medical, surgical and other health care professionals, as well as the patient. Results of studies conducted in the past few years have allowed experts to formulate guidelines that can improve clinical outcomes. We conducted a narrative review of the literature on treat- ment of DFO, with an emphasis on studies published in the last two years, especially regarding antimicrobial therapies and surgical approached to treatment of DFO, supplemented by our own extensive clinical and research experience in this field. Read More

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Genistein improves systemic metabolism and enhances cold resistance by promoting adipose tissue beiging.

Biochem Biophys Res Commun 2021 Apr 26;558:154-160. Epub 2021 Apr 26.

Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, 410010, China. Electronic address:

Genistein, a naturally occurring phytoestrogen and a member of the large class of compounds known as isoflavones, exerts protective effects in several diseases. Recent studies indicate that genistein plays a critical role in controlling body weight, obesity-associated insulin resistance, and metabolic disorders, but its target organs in reversing obesity and related pathological conditions remain unclear. In this study, we showed that mice supplemented with 0. Read More

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Acute pre-exercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans.

J Appl Physiol (1985) 2021 Apr 29. Epub 2021 Apr 29.

Department of Movement and Sports Sciences, Ghent University, Belgium.

Classic in vitro experiments (Severin's phenomenon) demonstrated that acute carnosine supplementation may potentiate muscle contractility. However, upon oral ingestion, carnosine is readily degraded in human plasma by the highly active serum carnosinase-1 (CN1). We developed a novel strategy to circumvent CN1 by pre-exercise ingestion of combined carnosine (CARN) and anserine (ANS), the methylated analog with similar biochemical properties but more resistant to CN1. Read More

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The Oxygen Uptake Plateau-A Critical Review of the Frequently Misunderstood Phenomenon.

Sports Med 2021 Apr 29. Epub 2021 Apr 29.

Department Medicine, Training and Health, Institute of Sports Science and Motologie, Philipps-University Marburg, Jahnstr. 12, 35037, Marburg, Germany.

A flattening of the oxygen uptake-work rate relationship at severe exercise indicates the achievement of maximum oxygen uptake [Formula: see text]. Unfortunately, a distinct plateau [Formula: see text] at [Formula: see text]is not found in all participants. The aim of this investigation was to critically review the influence of research methods and physiological factors on the [Formula: see text] incidence. Read More

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