211 results match your criteria subsp damselae


Complete, closed and curated genome sequences of subsp. isolates from Australia indicate mobilome-driven localized evolution and novel pathogenicity determinants.

Microb Genom 2021 Apr;7(4)

School of Biological Sciences, University of Queensland, Brisbane, Queensland 4072, Australia.

Despite the recent advances in sequencing technologies, the complete assembly of multi-chromosome genomes of the , often containing several plasmids, remains challenging. Using a combination of Oxford Nanopore MinION long reads and short Illumina reads, we fully sequenced, closed and curated the genomes of two strains of a primary aquatic pathogen subsp. isolated in Australia. Read More

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Methionine and Tryptophan Play Different Modulatory Roles in the European Seabass () Innate Immune Response and Apoptosis Signaling-An Study.

Front Immunol 2021 15;12:660448. Epub 2021 Mar 15.

Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Matosinhos, Portugal.

The range of metabolic pathways that are dependent on a proper supply of specific amino acids (AA) unveils their importance in the support of health. AA play central roles in key pathways vital for immune support and individual AA supplementation has shown to be able to modulate fish immunity. trials are important tools to evaluate the immunomodulatory role of AA, and the present study was conceived to evaluate methionine and tryptophan roles in immune-related mechanisms aiming to understand their effects in leucocyte functioning and AA pathways. Read More

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Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer).

Fish Shellfish Immunol 2021 Apr 6;111:179-188. Epub 2021 Feb 6.

International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Southern Taiwan Fish Diseases Research Centre, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan; Research Centre for Animal Biologics, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan. Electronic address:

Photobacterium damselae subsp. piscicida (Phdp) is the causative agent of photobacteriosis in marine fish and is responsible for huge losses to marine aquaculture worldwide. Efforts have been made to develop a vaccine against this disease. Read More

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A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. Cleaves the Peptidoglycan of Potentially Competing Bacteria.

mSphere 2021 02 3;6(1). Epub 2021 Feb 3.

Fish Immunology and Vaccinology Group, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Porto, Portugal

Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope. PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG. This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from subsp. Read More

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February 2021

Convergent Total Synthesis of the Siderophore Piscibactin as Its Ga Complex.

Org Lett 2021 01 23;23(2):340-345. Epub 2020 Dec 23.

Departamento de Química, Facultade de Ciencias e Centro de Investigacións Científicas Avanzadas (CICA), Agrupación Estratéxica CICA-INIBIC, Universidade da Coruña, A Coruña E-15071, Spain.

The siderophore piscibactin is a key virulence factor involved in the iron uptake of pathogenic bacteria subsp. and , responsible for the fish diseases photobacterioisis (pasteurellosis) and vibriosis, respectively. A convergent total synthesis of its Ga complex using l-/d-cysteine as chiral agents and Meldrum's acid is described. Read More

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January 2021

Diverse Horizontally-Acquired Gene Clusters Confer Sucrose Utilization to Different Lineages of the Marine Pathogen subsp. .

Genes (Basel) 2020 10 22;11(11). Epub 2020 Oct 22.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

The ability to metabolize sucrose is a variable trait within the family Vibrionaceae. The marine bacterium Photobacterium damselae subsp. damselae (Pdd), pathogenic for marine animals and humans, is generally described as negative for sucrose utilization (Scr). Read More

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October 2020

Intestinal Explant Cultures from Gilthead Seabream ( Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet.

Int J Mol Sci 2020 Oct 14;21(20). Epub 2020 Oct 14.

Aquaculture and Biodiversity Research Group, Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain.

The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. Read More

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October 2020

Exposure of the Opportunistic Marine Pathogen subsp. to Human Body Temperature Is a Stressful Condition That Shapes the Transcriptome, Viability, Cell Morphology, and Virulence.

Front Microbiol 2020 24;11:1771. Epub 2020 Jul 24.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

subsp. (), an important pathogen for marine animals, is also an opportunistic human pathogen that can cause fatal necrotizing fasciitis. The regulatory changes triggered by the temperature shift experienced by this marine pathogen upon entering the human body, are completely unknown. Read More

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Dietary arginine and citrulline supplementation modulates the immune condition and inflammatory response of European seabass.

Fish Shellfish Immunol 2020 Nov 13;106:451-463. Epub 2020 Aug 13.

Centro Interdisciplinar de Investigação Marinha e Ambiental, Matosinhos, Portugal; Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal. Electronic address:

The present study was designed to determine the modulatory effects of arginine and citrulline dietary supplementation on the immune condition and inflammatory response of European seabass, Dicentrarchus labrax. Four diets were manufactured: a control diet (CTRL) was formulated to meet the indispensable amino acids profile established for seabass. Based on this formulation, three other diets were supplemented with l-arginine at two different levels (0. Read More

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November 2020

Effects of aqueous and ethanolic leaf extracts from drumstick tree (Moringa oleifera) on gilthead seabream (Sparus aurata L.) leucocytes, and their cytotoxic, antitumor, bactericidal and antioxidant activities.

Fish Shellfish Immunol 2020 Nov 31;106:44-55. Epub 2020 Jul 31.

Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. Electronic address:

Aqueous and ethanolic extracts of drumstick, Moringa oleifera, leaves were evaluated in vitro to ascertain their principal active components and determine their immunostimulant, cytotoxic, antitumoral, bactericidal and antioxidant activities. Phytochemical screening of M. oleifera leaf extracts showed a greater abundance of phenolic and cyanogenic glycosides in aqueous than in ethanolic extracts, characterized by several flavonoids, condensed tannins and saponins. Read More

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November 2020

Use of bacteriophage vB_Pd_PDCC-1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail (Seriola rivoliana) eggs.

J Appl Microbiol 2020 Dec 3;129(6):1497-1510. Epub 2020 Jul 3.

CONACYT-CIBNOR, Av. Instituto Politécnico Nacional, La Paz Baja California Sur, Mexico.

Aims: This study describes the effect of phage therapy on hatching of longfin yellowtail (Seriola rivoliana) eggs challenged with Photobacterium damselae subsp. damselae.

Methods And Results: A lytic phage (vB_Pd_PDCC-1) against P. Read More

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December 2020

Genotypic diversity, and molecular and pathogenic characterization of Photobacterium damselae subsp. piscicida isolated from different fish species in Taiwan.

J Fish Dis 2020 Jul 17;43(7):757-774. Epub 2020 May 17.

International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan.

Photobacteriosis, caused by Photobacterium damselae subsp. piscicida (Phdp), is a serious disease in marine fish species worldwide. To date, the epidemiological characterization of this pathogen in Taiwan remains limited. Read More

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Highly Transferable pAQU-Related Plasmids Encoding Multidrug Resistance Are Widespread in the Human and Fish Pathogen Photobacterium damselae subsp. damselae in Aquaculture Areas in the Black Sea.

Microb Ecol 2020 Oct 8;80(3):507-518. Epub 2020 May 8.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Galicia, Spain.

The marine bacterium Photobacterium damselae subsp. damselae is a pathogen that causes disease in diverse marine animals, and is also a serious opportunistic human pathogen that can cause fatal infections. Strains of this pathogen isolated from diseased European sea bass in aquaculture facilities in the Turkish coast of the Black Sea were found to exhibit reduced sensitivity to multiple antimicrobials. Read More

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October 2020

A novel research on isolation and characterization of Photobacterium damselae subsp. damselae from Pacific white shrimp, Penaeus vannamei, displaying black gill disease cultured in China.

J Fish Dis 2020 May 20;43(5):551-559. Epub 2020 Mar 20.

Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.

In June 2019, massive mortalities of cultured Penaeus vannamei occurred in a local farm in Hainan Province, China. The diseased shrimp displayed evident black gills. Three bacterial strains 20190611001, 20190611007 and 20190611022 were isolated from hepatopancreas and gills of the diseased shrimp and identified as Photobacterium damselae subsp. Read More

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Inclusion of dietary Ulva ohnoi 5% modulates Solea senegalensis immune response during Photobacterium damselae subsp. piscicida infection.

Fish Shellfish Immunol 2020 May 5;100:186-197. Epub 2020 Mar 5.

Departamento de Microbiología, Universidad de Málaga, Andalucia Tech, Campus de Teatinos s/n, 29071, Málaga, Spain. Electronic address:

Macroalgae represent valuable sources of functional ingredients for fish diets, and the influence of supplemented aquafeeds on growth performance has been studied for some fish and seaweed species. In the present work, the potential immunomodulation exerted by U. ohnoi (5%) as dietary ingredient was investigated in Senegalese sole. Read More

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Effects of the sulfated polysaccharide ulvan from Ulva ohnoi on the modulation of the immune response in Senegalese sole (Solea senegalensis).

Fish Shellfish Immunol 2020 May 27;100:27-40. Epub 2020 Feb 27.

IFAPA Centro El Toruño, Camino Tiro Pichón s/n, 11500, El Puerto de Santa María, Cádiz, Spain. Electronic address:

Sulfated polysaccharides derived from green seaweeds exhibit many beneficial biological activities and have great potential to be used as nutraceutical in aquaculture. In this work, we evaluated the effects of the sulfated polysaccharide ulvan from Ulva ohnoi on Senegalese sole (Solea senegalensis) juveniles at the transcriptomic level. Cytotoxicity assay performed in liver primary cell cultures from sole determined that the different ulvan concentrations assayed did not impair cell viability. Read More

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Author Correction: Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida.

Sci Rep 2020 Jan 22;10(1):1266. Epub 2020 Jan 22.

Fish Immunology and Vaccinology Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.

An amendment to this paper has been published and can be accessed via a link at the top of the paper. Read More

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January 2020

Protective effects of seaweed supplemented diet on antioxidant and immune responses in European seabass (Dicentrarchus labrax) subjected to bacterial infection.

Sci Rep 2019 11 6;9(1):16134. Epub 2019 Nov 6.

CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Av. General Norton de Matos s/n, 4450-208, Matosinhos, Portugal, Portugal.

European seabass (Dicentrarchus labrax) production is often hampered by bacterial infections such as photobacteriosis caused by Photobacterium damselae subsp. piscicida (Phdp). Since diet can impact fish immunity, this work investigated the effect of dietary supplementation of 5% Gracilaria sp. Read More

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November 2019

Outer membrane protein FrpA, the siderophore piscibactin receptor of Photobacterium damselae subsp. piscicida, as a subunit vaccine against photobacteriosis in sole (Solea senegalensis).

Fish Shellfish Immunol 2019 Nov 30;94:723-729. Epub 2019 Sep 30.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, 15782, Spain. Electronic address:

Photobacteriosis caused by Photobacterium damselae subsp. piscicida (Pdp) remains one of the main infectious diseases affecting cultured fish in Mediterranean countries. Diverse vaccine formulations based in the use of inactivated bacterial cells have been used with unsatisfactory results, especially in newly cultured species like sole (Solea senegalensis). Read More

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November 2019

The Fish Pathogen Under Iron Deprivation Produces the Siderophore Piscibactin.

Microorganisms 2019 Sep 3;7(9). Epub 2019 Sep 3.

Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología and Instituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by . Read More

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September 2019

Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication.

Cell Microbiol 2020 01 11;22(1):e13109. Epub 2019 Sep 11.

Fish Immunology and Vaccinology Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.

Apoptosis-inducing protein of 56 kDa (AIP56) is a major virulence factor of Photobacterium damselae subsp. piscicida, a gram-negative pathogen that infects warm water fish species worldwide and causes serious economic losses in aquacultures. AIP56 is a single-chain AB toxin composed by two domains connected by an unstructured linker peptide flanked by two cysteine residues that form a disulphide bond. Read More

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January 2020

Fatal Photobacterium damselae-induced enteritis in a leatherback turtle Dermochelys coriacea.

Dis Aquat Organ 2019 Aug;135(2):151-156

Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California-Davis, Davis, California 95616, USA.

Stranded leatherback turtle Dermochelys coriacea complete pathology reports are rare, and the cause of mortality is difficult to determine in many cases. We conducted a complete pathological study of a stranded leatherback turtle from the western Mediterranean. The main finding was a fibrino-necrotizing enteritis with associated bacteria which were identified as Photobacterium damselae subsp. Read More

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Phobalysin: Fisheye View of Membrane Perforation, Repair, Chemotaxis and Adhesion.

Toxins (Basel) 2019 07 16;11(7). Epub 2019 Jul 16.

Institute of Medical Microbiology and Hygiene, University Medical Center, Johannes Gutenberg-University, 55128 Mainz, Germany.

Phobalysin P (PhlyP, for photobacterial lysin encoded on a plasmid) is a recently described small β-pore forming toxin of subsp. This organism, belonging to the family of is an emerging pathogen of fish and various marine animals, which occasionally causes life-threatening soft tissue infections and septicemia in humans. By using genetically modified strains, PhlyP was found to be an important virulence factor. Read More

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Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida.

Sci Rep 2019 06 21;9(1):9019. Epub 2019 Jun 21.

Fish Immunology and Vaccinology Group, IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.

AIP56 (apoptosis inducing protein of 56 kDa) is a key virulence factor secreted by virulent strains of Photobacterium damselae subsp. piscicida (Phdp), a Gram-negative bacterium that causes septicemic infections in several warm water marine fish species. AIP56 is systemically disseminated during infection and induces massive apoptosis of host macrophages and neutrophils, playing a decisive role in the disease outcome. Read More

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Fish photobacteriosis-The importance of rapid and accurate identification of Photobacterium damselae subsp. piscicida.

J Fish Dis 2019 Aug 13;42(8):1201-1209. Epub 2019 Jun 13.

Laboratory for Aquaculture Biotechnology, Rudjer Bošković Institute, Zagreb, Croatia.

MALDI-TOF MS was tested for the identification of Photobacterium damselae subsp. piscicida on isolates grown on two media, cultured at three incubation times and applied on the target plate by the direct sample spotting (DS), by the on-target extraction (OTE) and by the full extraction (FE) method, in triplicates. The identification of samples grown on blood agar (BA) outperformed identification on tryptic soya agar (TSA) by 0. Read More

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Draft Genome Sequences of Photobacterium damselae subsp. piscicida SNW-8.1 and PP3, Two Fish-Isolated Strains Containing a Type III Secretion System.

Microbiol Resour Announc 2019 May 23;8(21). Epub 2019 May 23.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, Santiago de Compostela, Spain

Here, we report the draft genome sequences of two strains of the fish pathogen subsp. , isolated from (SNW-8.1) and (PP3). Read More

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Dietary Tryptophan Induces Opposite Health-Related Responses in the Senegalese Sole () Reared at Low or High Stocking Densities With Implications in Disease Resistance.

Front Physiol 2019 1;10:508. Epub 2019 May 1.

Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal.

High rearing densities are typical conditions of both inland and onshore intensive aquaculture units. Despite obvious drawbacks, this strategy is nonetheless used to increase production profits. Such conditions inflict stress on fish, reducing their ability to cope with disease, bringing producers to adopt therapeutic strategies. Read More

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The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen subsp. .

Front Microbiol 2019 24;10:897. Epub 2019 Apr 24.

Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela - USC, Santiago de Compostela, Spain.

The RstB histidine kinase of the two component system RstAB positively regulates the expression of damselysin (Dly), phobalysin P (PhlyP) and phobalysin C (PhlyC) cytotoxins in the fish and human pathogen subsp. , a marine bacterium of the family . However, the function of the predicted cognate response regulator RstA has not been studied so far, and the role of the RstAB system in other cell functions and phenotypes remain uninvestigated. Read More

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Complete Genome Sequence of Subsp. Strain SSPD1601 Isolated from Deep-Sea Cage-Cultured with Septic Skin Ulcer.

Int J Genomics 2019 10;2019:4242653. Epub 2019 Apr 10.

Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academic of Fishery Sciences, Qingdao 266071, China.

subsp. (PDD) is a Gram-negative bacterium that can infect a variety of aquatic organisms and humans. Based on an epidemiological investigation conducted over the past 3 years, PDD is one of the most important pathogens causing septic skin ulcer in deep-sea cage-cultured in the Huang-Bohai Sea area and present throughout the year with high abundance. Read More

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Molecular cloning of IL-6, IL-10, IL-11, IFN-ɤ and modulation of pro- and anti-inflammatory cytokines in cobia (Rachycentron canadum) after Photobacterium damselae subsp. piscicida infection.

Comp Biochem Physiol B Biochem Mol Biol 2019 Apr 18;230:10-18. Epub 2019 Jan 18.

Laboratory of Molecular Fish Immunology and Genetics, Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan. Electronic address:

Photobacterium damselae subsp. piscicida (P. damselae subsp. Read More

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