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Diabetes status modifies the long-term effect of lipoprotein-associated phospholipase A2 on major coronary events.

Diabetologia 2021 Sep 25. Epub 2021 Sep 25.

Division of Population Health and Genomics, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK.

Aims/hypothesis: Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity has an independent prognostic association with major coronary events (MCE). However, no study has investigated whether type 2 diabetes status modifies the effect of Lp-PLA2 activity or inhibition on the risk of MCE. We investigate the interaction between diabetes status and Lp-PLA2 activity with risk of MCE. Read More

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September 2021

Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9.

Ecotoxicol Environ Saf 2021 Sep 21;225:112789. Epub 2021 Sep 21.

School of Civil Engineering, Yulin University, Yulin 719000, China.

A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated. Read More

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September 2021

Elevated placental histone H3K4 methylation via upregulated histone methyltransferases SETD1A and SMYD3 in preeclampsia and its possible involvement in hypoxia-induced pathophysiological process.

Placenta 2021 Sep 12;115:60-69. Epub 2021 Sep 12.

Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.

Introduction: Disturbance in placental epigenetic regulation contributes to the pathogenesis of preeclampsia (PE). Although aberrant placental DNA methylation status in PE has been thoroughly studied, the role of histone modifications, including histone methylation, in PE remains unclear. Moreover, no study has ever reported the association between PE and placental histone methylation status by focusing on histone methyltransferases. Read More

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September 2021

Gastric microbiota in patients with Helicobacter pylori-negative gastric MALT lymphoma.

Medicine (Baltimore) 2021 Sep;100(38):e27287

Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Abstract: To investigate the mucosal microbiota in the stomach of patients with Helicobacter pylori-negative mucosa-associated lymphoid tissue (MALT) lymphoma by means of metagenomic analysis.Although some gastric MALT lymphomas are associated with the presence of H. pylori, other gastric MALT lymphomas occur independently of H. Read More

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September 2021

Abnormalities in normal-appearing white matter from which multiple sclerosis lesions arise.

Brain Commun 2021 10;3(3):fcab176. Epub 2021 Aug 10.

Biogen Digital Health, Biogen, Cambridge, MA 02142, USA.

Normal-appearing white matter is far from normal in multiple sclerosis; little is known about the precise pathology or spatial pattern of this alteration and its relation to subsequent lesion formation. This study was undertaken to evaluate normal-appearing white matter abnormalities in brain areas where multiple sclerosis lesions subsequently form, and to investigate the spatial distribution of normal-appearing white matter abnormalities in persons with multiple sclerosis. Brain MRIs of pre-lesion normal-appearing white matter were analysed in participants with new T2 lesions, pooled from three clinical trials: SYNERGY (NCT01864148; = 85 with relapsing multiple sclerosis) was the test data set; ASCEND (NCT01416181; = 154 with secondary progressive multiple sclerosis) and ADVANCE (NCT00906399; = 261 with relapsing-remitting multiple sclerosis) were used as validation data sets. Read More

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Cross-cultural Adaptation of the Health Sciences Evidence-based Practice Questionnaire into Japanese and Its Test-Retest Reliability in Undergraduate Students.

Prog Rehabil Med 2021 27;6:20210034. Epub 2021 Aug 27.

Balearic Islands Health Research Institute (IdISBa), Palma de Mallorca, Spain.

Objectives: The Health Sciences Evidence-Based Practice (HS-EBP) questionnaire was recently developed for measuring five constructs of evidence-based clinical practice among Spanish health professionals by applying content and construct validity investigation. The current study aims to undertake a cross-cultural adaptation of the HS-EBP into Japanese and to investigate the internal consistency and test-retest reliability of the Japanese HS-EBP among undergraduate students of nursing and physical and occupational therapies.

Methods: Cross-cultural adaptation was undertaken by following Beaton's five-step process. Read More

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Qualitative Behavioral Assessment in Juvenile Farmed Atlantic Salmon (): Potential for On-Farm Welfare Assessment.

Front Vet Sci 2021 7;8:702783. Epub 2021 Sep 7.

Animal and Veterinary Sciences, SRUC (Scotland's Rural College), Edinburgh, United Kingdom.

There is a growing scientific and legislative consensus that fish are sentient, and therefore have the capacity to experience pain and suffering. The assessment of the welfare of farmed fish is challenging due to the aquatic environment and the number of animals housed together. However, with increasing global production and intensification of aquaculture comes greater impetus for developing effective tools which are suitable for the aquatic environment to assess the emotional experience and welfare of farmed fish. Read More

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September 2021

Evaluation of Angelica decursiva reference genes under various stimuli for RT-qPCR data normalization.

Sci Rep 2021 Sep 23;11(1):18993. Epub 2021 Sep 23.

School of Pharmacy, Jiangsu Health Vocational College, Nanjing, Jiangsu, China.

Angelica decursiva is one of the lending traditional Chinese medicinal plants producing coumarins. Notably, several studies have focused on the biosynthesis and not the RT-qPCR (quantitative real-time reverse transcription polymerase chain reaction) study of coumarins. This RT-qPCR technique has been extensively used to investigate gene expression levels in plants and the selection of reference genes which plays a crucial role in standardizing the data form the RT-qPCR analysis. Read More

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September 2021

Changes in magnetic resonance imaging relaxation time on postmortem magnetic resonance imaging of formalin-fixed human normal heart tissue.

BMC Med Imaging 2021 Sep 23;21(1):134. Epub 2021 Sep 23.

Department of Radiology, Faculty of Medical Science, University of Fukui, 23-3 Matsuoka Shimoaizuki, Eiheiji-cho, Yoshida-gun, Fukui, 910-1193, Japan.

Background: Postmortem magnetic resonance imaging (MRI) has been used to investigate the cause of death, but due to time constraints, it is not widely applied to the heart. Therefore, MRI analysis of the heart after formalin fixation was previously performed. However, the changes in MRI signal values based on the fixation time of formalin were not investigated. Read More

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September 2021

Osmoprotectant and antioxidant effects of new synthesized 6-(2-hydroxyethyl)cyclohex-3-enol on barley under drought stress.

Biol Futur 2021 Jun 7;72(2):241-249. Epub 2021 Jan 7.

Faculty of Medicine, Girne American University Drive, Karmi Campus, 10, Karaoglanoglu, Kyrenia, Mersin, Cyprus.

The aim of present study was synthesize 6-(2-hydroxyethyl)cyclohex-3-enol (11) and investigate its antioxidant properties in barley plants under drought stress. For this aim, 1,4-cyclohexadiene (7) was subjected to [2 + 2] ketene addition reaction with dichloro ketene and the chlorine atoms were reduced. After that, the cyclobutanone ring was converted to a lactone ring and the lactone ring was reduced with LiAlH. Read More

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Kynureninase contributes to the pathogenesis of psoriasis through pro-inflammatory effect.

J Cell Physiol 2021 Sep 22. Epub 2021 Sep 22.

Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China.

Kynureninase (KYNU) is a key enzyme in the tryptophan metabolism pathway with elevated expression in psoriatic lesions relative to normal skin. However, whether KYNU contributes to the pathogenesis of psoriasis remains unknown. We sought to investigate the role of KYNU in psoriasis and its possible regulation mechanism. Read More

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September 2021

LncRNA NEAT1 Promotes Gastric Cancer Progression Through miR-17-5p/TGFβR2 Axis Up-Regulated Angiogenesis.

Front Cell Dev Biol 2021 6;9:705697. Epub 2021 Sep 6.

Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Background: Long non-coding RNAs (lncRNAs) have been indicated to play critical roles in gastric cancer (GC) tumorigenesis and progression. However, their roles in GC remain to be further elucidated.

Methods: RT-qPCR and fluorescence hybridzation (FISH) were conducted to detect the expression of lncRNA NEAT1 in GC tissues and cell lines. Read More

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September 2021

Phantom Limb Pain: Feeling Sensation from a Limb that is No Longer Present and What it Can Reveal About Our Brain Anatomy.

Michele L Lemons

J Undergrad Neurosci Educ 2021 20;19(2):C1-C12. Epub 2021 Jun 20.

Department of Biological and Physical Sciences, Assumption University, Worcester, MA 01609.

This is a flexible, interrupted video case that uses phantom limb pain as a platform to investigate brain anatomy with a focus on somatosensory cortical mapping and the homunculus. The case begins with a video of neurologist Dr. V. Read More

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Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteins.

Proc Natl Acad Sci U S A 2021 09;118(39)

Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853;

As a common protein modification, asparagine-linked (linked) glycosylation has the capacity to greatly influence the biological and biophysical properties of proteins. However, the routine use of glycosylation as a strategy for engineering proteins with advantageous properties is limited by our inability to construct and screen large collections of glycoproteins for cataloguing the consequences of glycan installation. To address this challenge, we describe a combinatorial strategy termed shotgun scanning glycomutagenesis in which DNA libraries encoding all possible glycosylation site variants of a given protein are constructed and subsequently expressed in glycosylation-competent bacteria, thereby enabling rapid determination of glycosylatable sites in the protein. Read More

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September 2021

Finding Treatment Effects in Alzheimer Trials in the Face of Disease Progression Heterogeneity.

Neurology 2021 06;96(22):e2673-e2684

From the Department of Neurology, Alzheimer Center Amsterdam, Amsterdam Neuroscience, Amsterdam UMC (R.J.J., S.A.M.S., W.M.V.d.F., P.S., P.J.V., B.M.T.), and Clinical Developmental Psychology & Clinical Neuropsychology (S.A.M.S.), VU University; Alzheimer Center Limburg, School for Mental Health and Neuroscience (P.J.V.), Maastricht University, the Netherlands; and Department of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics (P.J.V.), Karolinska Institutet, Stockholm, Sweden.

Objective: To investigate the influence of heterogeneity in disease progression for detecting treatment effects in Alzheimer disease (AD) trials, using a simulation study.

Methods: Individuals with an abnormal amyloid PET scan, diagnosis of mild cognitive impairment or dementia, baseline Mini-Mental State Examination (MMSE) score ≥24, global Clinical Dementia Rating (CDR) score of 0.5, and ≥1 follow-up cognitive assessment were selected from the Alzheimer's Disease Neuroimaging Initiative database (n = 302, age 73 ± 6. Read More

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Protein Production and Purification of a Codon-Optimized Human NGN3 Transcription Factor from E. coli.

Protein J 2021 Sep 22. Epub 2021 Sep 22.

Laboratory for Stem Cell Engineering and Regenerative Medicine, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.

Neurogenin 3 (NGN3) transcription factor is vital for the development of endocrine cells of the intestine and pancreas. NGN3 is also critical for the neural precursor cell determination in the neuroectoderm. Additionally, it is one of the vital transcription factors for deriving human β-cells from specialized somatic cells. Read More

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September 2021

Ischiofemoral impingement: the evolutionary cost of pelvic obstetric adaptation.

J Hip Preserv Surg 2020 Dec 8;7(4):677-687. Epub 2021 Feb 8.

Department of Human Genetics, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.

The risk for ischiofemoral impingement has been mainly related to a reduced ischiofemoral distance and morphological variance of the femur. From an evolutionary perspective, however, there are strong arguments that the condition may also be related to sexual dimorphism of the pelvis. We, therefore, investigated the impact of gender-specific differences in anatomy of the ischiofemoral space on the ischiofemoral clearance, during static and dynamic conditions A random sampling Monte-Carlo experiment was performed to investigate ischiofemoral clearance during stance and gait in a large ( = 40 000) virtual study population, while using gender-specific kinematics. Read More

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December 2020

Genomic and Phenotypic Characteristics for Infections.

Infect Drug Resist 2021 14;14:3721-3726. Epub 2021 Sep 14.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.

Background: (VV) is a causative agent of foodborne diseases with high mortality. The aim of this study was to investigate the genomic and phenotypic profiles of VV.

Methods: Six VV isolates were collected and conducted whole-genome sequencing. Read More

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September 2021

The Mediterranean diet and dietary approach to stop hypertension (DASH)-style diet are differently associated with lipid profile in a large sample of Iranian adults: a cross-sectional study of Shahedieh cohort.

BMC Endocr Disord 2021 Sep 22;21(1):192. Epub 2021 Sep 22.

Nutrition and Food Security Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background: The association between the Mediterranean diet (MED) or dietary approach to stop hypertension (DASH) and cardiovascular disease (CVD) risk factors is well-documented. Nevertheless, a consistent relationship with the Middle East population has yet to be known. Thus, we aimed to investigate the association between DASH/MED and blood lipids in Iranian adults. Read More

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September 2021

Xiaoyu Xiezhuo Drink Protects against Ischemia-Reperfusion Acute Kidney Injury in Aged Mice through Inhibiting the TGF-1/Smad3 and HIF1 Signaling Pathways.

Biomed Res Int 2021 9;2021:9963732. Epub 2021 Sep 9.

Department of Nephrology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang, China.

Acute kidney injury (AKI) is responsible for significant mortality among hospitalized patients that is especially troubling aged people. An effective self-made Chinese medicine formula, Xiaoyu Xiezhuo Drink (XXD), displayed therapeutic effects on AKI. However, the compositions and underlying mechanisms of XXD remain to be elucidated. Read More

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September 2021

Safety and preliminary efficacy of sequential multiple ascending doses of solnatide to treat pulmonary permeability edema in patients with moderate-to-severe ARDS-a randomized, placebo-controlled, double-blind trial.

Trials 2021 Sep 20;22(1):643. Epub 2021 Sep 20.

Department of Anesthesiology, University Hospital of Ludwig-Maximilians-University (LMU), Munich, Germany.

Background: Acute respiratory distress syndrome (ARDS) is a complex clinical diagnosis with various possible etiologies. One common feature, however, is pulmonary permeability edema, which leads to an increased alveolar diffusion pathway and, subsequently, impaired oxygenation and decarboxylation. A novel inhaled peptide agent (AP301, solnatide) was shown to markedly reduce pulmonary edema in animal models of ARDS and to be safe to administer to healthy humans in a Phase I clinical trial. Read More

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September 2021

Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.

Cancer Cell Int 2021 Sep 20;21(1):505. Epub 2021 Sep 20.

Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

Aim: This study aimed to explore the mechanism of LncRNA urothelial carcinoma-associated 1 (UCA1) promoting cisplatin resistance in lung adenocarcinoma (LUAD).

Method: The UCA1 expression level in LUAD cell lines was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). We overexpressed UCA1 in A549 cells and downregulated UCA1 in A549/DDP cells by the lentivirus‑mediated technique. Read More

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September 2021

Extra-pair paternity in the wood-feeding cockroach Cryptocercus punctulatus Scudder: Social but not genetic monogamy.

Mol Ecol 2021 Sep 20. Epub 2021 Sep 20.

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695-7613, United States.

Subsocial Cryptocercus cockroaches are sister group to termites and considered socially monogamous. Because genetic monogamy is a suggested requirement for evolution of cooperative breeding/eusociality, particularly in hymenopterans, clarification of Cryptocercus mating biology would help illuminate evolutionary trends in eusocial insects. To investigate possible extra-pair paternity in C. Read More

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September 2021

Granulocyte colony-stimulating factor promotes an aggressive phenotype of colon and breast cancer cells with biochemical changes investigated by single-cell Raman microspectroscopy and machine learning analysis.

Analyst 2021 Sep 20. Epub 2021 Sep 20.

Department of Biological Engineering, Utah State University, Logan, UT 84322, USA.

Granulocyte colony-stimulating factor (G-CSF) is produced at high levels in several cancers and is directly linked with metastasis in gastrointestinal (GI) cancers. In order to further understand the alteration of molecular compositions and biochemical features triggered by G-CSF treatment at molecular and cell levels, we sought to investigate the long term treatment of G-CSF on colon and breast cancer cells measured by label-free, non-invasive single-cell Raman microspectroscopy. Raman spectrum captures the molecule-specific spectral signatures ("fingerprints") of different biomolecules presented on cells. Read More

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September 2021

ROCK1 Mediates Retinal Glial Cell Migration Promoted by Acrolein.

Front Med (Lausanne) 2021 3;8:717602. Epub 2021 Sep 3.

Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Acrolein is a highly reactive aldehyde that covalently binds to cellular macromolecules and subsequently modulates cellular function. Our previous study demonstrated that acrolein induces glial cell migration, a pathological hallmark of diabetic retinopathy; however, the detailed cellular mechanism remains unclear. The purpose of this study was to investigate the role of acrolein in retinal glial cell migration by focusing on rho-associated coiled-coil-containing protein kinases (ROCKs). Read More

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September 2021

Effects of carbamazepine and sodium valproate on efficacy, cognitive function and uric acid in epileptic patients with first generalized seizure.

Am J Transl Res 2021 15;13(8):9778-9783. Epub 2021 Aug 15.

Department of Intensive Care Unit, The Affiliated Changsha Hospital of Hunan Normal University Changsha 410006, Hunan, China.

Objective: To investigate the effects of carbamazepine and sodium valproate on efficacy, cognitive function and uric acid in epileptic patients with first generalized seizure.

Methods: 120 epilepsy patients with first generalized seizure who admitted to our hospital from March 2017 to March 2019, were selected and randomly divided into carbamazepine-group and sodium valproate-group, with 60 objects in each group. Both groups of patients received medication for one year. Read More

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Screening and identification of differentially expressed microRNAs in diffuse large B-cell lymphoma based on microRNA microarray.

Oncol Lett 2021 Nov 27;22(5):753. Epub 2021 Aug 27.

Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.

Diffuse large B-cell lymphoma (DLBCL) is the most common type of B-cell non-Hodgkin lymphoma in adults and the pathogenesis of DLBCL is multifactorial and complex. Understanding the molecular mechanisms involved in DLBCL is important to identify new therapeutic targets. The present study aimed to screen and identify differentially expressed microRNAs (miRNAs/miRs) between diffuse large B-cell lymphoma (DLBCL) and control [lymph node reactive hyperplasia (LRH)] groups, and to investigate whether miRNAs associated with DLBCL could serve as potential therapeutic targets. Read More

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November 2021

Diagnostic Accuracy of Female Pelvic Ultrasonography in Differentiating Precocious Puberty From Premature Thelarche: A Systematic Review and Meta-analysis.

Front Endocrinol (Lausanne) 2021 1;12:735875. Epub 2021 Sep 1.

Department of Family Medicine, Taipei Medical University Hospital, Taipei, Taiwan.

Background: The gonadotropin-releasing hormone (GnRH) stimulation test is the benchmark for diagnosing precocious puberty (PP). However, it is invasive, time-consuming, costly, and may create an unpleasant experience for participants. Moreover, some overlaps may occur between PP and premature thelarche (PT) in the early stage of PP. Read More

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September 2021

Exposure to ambient air pollution during childhood and subsequent risk of self-harm: A national cohort study.

Prev Med 2021 Nov 16;152(Pt 1):106502. Epub 2021 Sep 16.

Centre for Mental Health and Safety, Division of Psychology and Mental Health, Manchester Academic Health Sciences Centre, The University of Manchester, Manchester, UK; NIHR Greater Manchester Patient Safety Translational Research Centre, Manchester, UK.

A growing body of evidence indicates that exposure to air pollution not only impacts on physical health but is also linked with a deterioration in mental health. We conducted the first study to investigate exposure to ambient particulate matter with an aerodynamic diameter of less than 2.5 μm (PM) and nitrogen dioxide (NO) during childhood and subsequent self-harm risk. Read More

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November 2021

Serum Periostin Predicts Wheezing Exacerbation: A Prospective Study in Preschool Children with Recurrent Wheezing.

Int Arch Allergy Immunol 2021 Sep 17:1-8. Epub 2021 Sep 17.

Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Introduction: Wheezing is a common problem in preschool children. Currently, there are no reliable biomarkers that can predict subsequent wheezing in preschool children. This study aimed to compare serum periostin levels between preschool children with and without recurrent wheezing and investigate its utility for predicting acute wheezing exacerbation. Read More

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September 2021