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Protean Regulation of Leukocyte Function by Nuclear Lamins.

Trends Immunol 2021 Apr 27;42(4):323-335. Epub 2021 Feb 27.

Molecular Signaling Section, Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases (NIAID), Rockville, MD 20892, USA. Electronic address:

The leukocyte nucleus must be sufficiently elastic to squeeze through tissue barriers during migration, but not so collapsible as to risk damaging chromatin. The proper balance is struck in part by the composition of the nuclear lamina, a flexible meshwork composed mainly of intermediate filaments woven from type A and type B lamin proteins, that is located subjacent to the inner nuclear membrane. There is now increasing evidence that, in addition to influencing nuclear shape and stiffness and cell migration, lamins and lamin-interacting proteins may also interact functionally with chromatin to influence leukocyte gene expression, differentiation, and effector function, including T cell differentiation, B cell somatic hypermutation, and the formation of neutrophil extracellular traps (NETosis). Read More

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Wiad Lek 2020 ;73(12 cz 1):2612-2616


Objective: The aim: Thepaper wasaimedat thestudyof thebiomineralization processesofa permanentdental crown in thepostnatal period of histogenesis.

Patients And Methods: Materials and methods: The study involved 30 culled puppies aged 30-40 days. To study the histogenesis of the germs of the permanent tooth from the cuticular epithelium in the postnatal period microscopic, electron microscopic, immunohistochemical methods of study have been used. Read More

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February 2021

Extracellular vesicles: how they interact with endothelium, potentially contributing to metastatic cancer cell implants.

Murray M Bern

Clin Transl Med 2017 Sep 21;6(1):33. Epub 2017 Sep 21.

University of New Mexico Comprehensive Cancer Center, 1201 Camino de Salud, Albuquerque, NM, 87131, USA.

Extracellular vesicles (EV) are blebs of cellular membranes, which entrap small portions of subjacent cytosol. They are released from a variety of cells, circulate in the blood for an unknown length of time and come to rest on endothelial surfaces. They contribute to an array of physiologic pathways, the complexity of which is still being investigated. Read More

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September 2017

Ocular Lesions in Red-Tailed Hawks ( Buteo jamaicensis) With Naturally Acquired West Nile Disease.

Vet Pathol 2017 03 1;54(2):277-287. Epub 2016 Oct 1.

3 The Raptor Center, University of Minnesota, St Paul, MN, USA.

Ocular lesions are common in red-tailed hawks with West Nile (WN) disease. These lesions consist of pectenitis, choroidal or retinal inflammation, or retinal necrosis, but detailed investigation of the ocular lesions is lacking. Postmortem examination of the eyes of 16 red-tailed hawks with naturally acquired WN disease and 3 red-tailed hawks without WN disease was performed using histopathology, immunohistochemistry for West Nile virus (WNV) antigen, glial fibrillary acid protein, cleaved caspase-3, and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method. Read More

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Periosteal PTHrP Regulates Cortical Bone Remodeling During Fracture Healing.

Bone 2015 Dec 9;81:104-111. Epub 2015 Jul 9.

Department of Orthopaedics and Rehabilitation, Yale University, New Haven, CT 06520, USA. Electronic address:

Parathyroid hormone-related protein (PTHrP) is widely expressed in the fibrous outer layer of the periosteum (PO), and the PTH/PTHrP type I receptor (PTHR1) is expressed in the inner PO cambial layer. The cambial layer gives rise to the PO osteoblasts (OBs) and osteoclasts (OCs) that model/remodel the cortical bone surface during development as well as during fracture healing. PTHrP has been implicated in the regulation of PO modeling during development, but nothing is known as regards a role of PTHrP in this location during fracture healing. Read More

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December 2015

Decidualization and syndecan-1 knock down sensitize endometrial stromal cells to apoptosis induced by embryonic stimuli.

PLoS One 2015 1;10(4):e0121103. Epub 2015 Apr 1.

Department of Obstetrics/Gynecology and Reproductive Endocrinology and Infertility (UniKiD), Medical Center University of Duesseldorf, Duesseldorf, Germany.

Human embryo invasion and implantation into the inner wall of the maternal uterus, the endometrium, is the pivotal process for a successful pregnancy. Whereas disruption of the endometrial epithelial layer was already correlated with the programmed cell death, the role of apoptosis of the subjacent endometrial stromal cells during implantation is indistinct. The aim was to clarify whether apoptosis plays a role in the stromal invasion and to characterize if the apoptotic susceptibility of endometrial stromal cells to embryonic stimuli is influenced by decidualization and Syndecan-1. Read More

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Retinal vascular layers in macular telangiectasia type 2 imaged by optical coherence tomographic angiography.

JAMA Ophthalmol 2015 Jan;133(1):66-73

Vitreous Retina Macula Consultants of New York, New York.

Importance: Macular telangiectasia type 2 (MacTel 2) is a rare disease in which abnormalities of the retinal vasculature play a key role. The vascular abnormalities are typically evaluated using fluorescein angiography, a modality with known defects in imaging the deeper layers of the retinal vasculature. Angiography based on optical coherence tomography can image vessels based on flow characteristics without dye injection and may provide improved information concerning the pathophysiology of MacTel 2. Read More

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January 2015

Determination of the Young's modulus of the epicuticle of the smooth adhesive organs of Carausius morosus using tensile testing.

J Exp Biol 2014 Oct 11;217(Pt 20):3677-87. Epub 2014 Sep 11.

Department Cellular Neurobionics, Institute for Biology II, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany Institute of Biomedical Mechatronics, University of Linz, Altenbergerstraße 69, 4040 Linz, Austria.

Adhesive organs like arolia of insects allow these animals to climb on different substrates by creating high adhesion forces. According to the Dahlquist criterion, adhesive organs must be very soft, exhibiting an effective Young's modulus of below 100 kPa to adhere well to substrates. Such a low effective Young's modulus allows the adhesive organs to make almost direct contact with the substrate and results in van der Waals forces along with capillary forces. Read More

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October 2014

SDOCT thickness measurements of various retinal layers in patients with autosomal dominant optic atrophy due to OPA1 mutations.

Biomed Res Int 2013 19;2013:121398. Epub 2013 Aug 19.

Department of Ophthalmology, University Hospital of Cologne, 50924 Cologne, Germany.

Purpose: To specify thickness values of various retinal layers on macular spectral domain Optical Coherence Tomography (SDOCT) scans in patients with autosomal dominant optic atrophy (ADOA) compared to healthy controls.

Methods: SDOCT volume scans of 7 patients with ADOA (OPA-1 mutation) and 14 healthy controls were quantitatively analyzed using manual grading software. Mean thickness values for the ETDRS grid subfields 5-8 were calculated for the spaces neurosensory retina, retinal nerve fiber layer (RNFL), ganglion cell layer (GCL), a combined space of inner plexiform layer/outer plexiform layer/inner nuclear layer (IPL+INL+OPL), and a combined space of outer nuclear layer/photoreceptor layers (ONL+PL). Read More

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In vivo identification of alteration of inner neurosensory layers in branch retinal artery occlusion.

Br J Ophthalmol 2012 Feb 22;96(2):201-7. Epub 2011 Apr 22.

Department of Ophthalmology, Medical University of Vienna, Austria, Waehringer Guertel 18-20, Vienna A-1090, Austria.

Background/aims: To characterise the extension and progression of alteration of neurosensory layers following acute and chronic branch retinal artery occlusion (BRAO) in vivo using spectral-domain optical coherence tomography.

Methods: In this observational case series, eight eyes with acute BRAO and nine eyes with chronic BRAO were analysed using a Spectralis Heidelberg Retina Angiograph (HRA)+optical coherence tomography system including eye tracking. Patients with acute BRAO were examined within 36±5 h after primary event and at weekly/monthly intervals thereafter. Read More

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February 2012

Illustrative stream surfaces.

IEEE Trans Vis Comput Graph 2010 Nov-Dec;16(6):1329-38

Universität Leipzig.

Stream surfaces are an intuitive approach to represent 3D vector fields. In many cases, however, they are challenging objects to visualize and to understand, due to a high degree of self-occlusion. Despite the need for adequate rendering methods, little work has been done so far in this important research area. Read More

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December 2010

Fit of surgical guides--manufactured by InLab 3D.

Int J Comput Dent 2007 Oct;10(4):329-37

Department of Prosthodontics and Material Sciences, University of Leipzig, Germany.

Based on an exclusively virtual implant planning and positioning concept, the fit of surgical-guide-like workpieces was evaluated in an in-vitro pilot study, manufactured with the InLab 3D system. From 85 maxillary casts, 8 were randomly selected. The first right maxillary incisor was removed. Read More

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October 2007

Micromechanics of smooth adhesive organs in stick insects: pads are mechanically anisotropic and softer towards the adhesive surface.

J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2008 Apr 25;194(4):373-84. Epub 2008 Jan 25.

Department of Cellular Neurobionics, Institute of Biology 2, RWTH-Aachen, Kopernikusstr. 16, 52068, Aachen, Germany.

Animals have evolved adhesive structures on their legs to cling to the substrate during locomotion. Here we characterise the ultrastructure and mechanical properties of adhesive pads in Carausius morosus (Phasmatodea) using atomic force microscopy (AFM) as well as transmission and scanning electron microscopy (TEM, SEM). The smooth adhesive arolium has a soft cuticle consisting of principal rods, which branch into finer fibres near the surface. Read More

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Colonization of barley (Hordeum vulgare) with Salmonella enterica and Listeria spp.

FEMS Microbiol Ecol 2006 May;56(2):262-71

GSF-National Research Center for Environment and Health, Department of Rhizosphere Biology, Institute of Soil Ecology, Neuherberg, Germany.

Colonization of barley plants by the food-borne pathogens Salmonella enterica serovar typhimurium and three Listeria spp. (L. monocytogenes, L. Read More

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FGF8 initiates inner ear induction in chick and mouse.

Genes Dev 2005 Mar;19(5):603-13

Sensory Development, Riken Center for Developmental Biology, Chuo-ku, Kobe 650-0047, Japan.

In both chick and mouse, the otic placode, the rudiment of the inner ear, is induced by at least two signals, one from the cephalic paraxial mesoderm and the other from the neural ectoderm. In chick, the mesodermal signal, FGF19, induces neural ectoderm to express additional signals, including WNT8c and FGF3, resulting in induction of the otic placode. In mouse, mesodermal Fgf10 acting redundantly with neural Fgf3 is required for induction of the placode. Read More

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Tooth development in a scincid lizard, Chalcides viridanus (Squamata), with particular attention to enamel formation.

Cell Tissue Res 2005 Jan 3;319(1):71-89. Epub 2004 Nov 3.

Equipe Evolution & Développement du Squelette, Université Paris 6, CNRS FRE 2696, Case 7077, 7 Quai St.-Bernard, 75251 Paris cedex 05, France.

Comparative analysis of tooth development in the main vertebrate lineages is needed to determine the various evolutionary routes leading to current dentition in living vertebrates. We have used light, scanning and transmission electron microscopy to study tooth morphology and the main stages of tooth development in the scincid lizard, Chalcides viridanus, viz., from late embryos to 6-year-old specimens of a laboratory-bred colony, and from early initiation stages to complete differentiation and attachment, including resorption and enamel formation. Read More

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January 2005

Analysis of detached human kinetochores.

Chromosoma 2003 Aug 23;112(2):96-102. Epub 2003 Jul 23.

Department of Cell Biology and Neuroscience, The University of South Alabama, Mobile, AL 36688, USA.

A method has recently been established for inducing the physical detachment of kinetochores from chromosomes in human HeLa cells, and was used in the studies reported here to investigate the organization and function of dissociated HeLa kinetochores. Immunofluorescence labeling demonstrated that the detached HeLa kinetochores were relatively intact, with the number of detached kinetochores being only moderately more than the diploid number of chromosomes in HeLa cells. In addition, the detached kinetochores could be labeled with antibodies specific for the inner kinetochore plate, outer kinetochore, and subjacent centromeric heterochromatin. Read More

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Morphology of the air-breathing stomach of the catfish Hypostomus plecostomus.

J Morphol 2003 Aug;257(2):147-63

Department of Comparative Anatomy, Institute of Zoology, Jagiellonian University, 30-060 Kraków, Poland.

Histological and ultrastructural investigations of the stomach of the catfish Hypostomus plecostomus show that its structure is different from that typical of the stomachs of other teleostean fishes: the wall is thin and transparent, while the mucosal layer is smooth and devoid of folds. The epithelium lining the whole internal surface of the stomach consists of several types of cells, the most prominent being flattened respiratory epithelial cells. There are also two types of gastric gland cells, three types of endocrine cells (EC), and basal cells. Read More

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Comparative ultrastructure of the root system in rhizocephalan barnacles (Crustacea: Cirripedia: Rhizocephala).

J Morphol 2001 Jul;249(1):9-42

Department of Ecology, Zoology Section, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.

Rhizocephalan barnacles are parasites of Crustacea. They lack even the rudiments of an alimentary canal, but infiltrate their hosts with a nutrient-absorbing system of rootlets. We review the ultrastructure of the rootlets using light microscopy, SEM, and TEM in nine species from five families, representing both suborders of the Rhizocephala: from the Kentrogonida Peltogaster paguri, P. Read More

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Proteasome inhibitors alter the orderly progression of DNA synthesis during S-phase in HeLa cells and lead to rereplication of DNA.

R Yamaguchi A Dutta

Exp Cell Res 2000 Nov;261(1):271-83

Division of Molecular Oncology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.

Replication of the mammalian genome occurs only once per cell cycle and is under strict spatiotemporal control. DNA synthesis first takes place in the inner nucleus and moves gradually to the area subjacent to the nuclear membrane as S-phase progresses. We found that proteasome inhibitors specifically reduce DNA synthesis from later replicating origins but not that from earlier replicating origins. Read More

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November 2000

CENP-G in neocentromeres and inactive centromeres.

Chromosoma 2000 ;109(5):328-33

Laboratorio di Citogenetica, Istituto G. Gaslini, Genoa, Italy.

CENP-G is a novel constitutive centromere-specific protein localized to the kinetochore inner plate and subjacent region. It has been identified as associating specifically with the alpha-1 subfamily of alpha-satellite DNA. In the present work, the localization of CENP-G was compared with that of other CENPs by immunofluorescence and fluorescence in situ hybridization. Read More

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February 2001

Ultrastructural analysis of folliculogenesis in the ovary of the yellow spotted stingray, Urolophus jamaicensis.

Ann Anat 1999 Mar;181(2):159-72

South Bend Center for Medical Education, Indiana University School of Medicine, Notre Dame, USA.

The ovary of the yellow spotted ray, Urolophus jamaicensis, is embedded in the epigonal gland, a lymphomyeloid organ. The covering of the ovary is composed of a germinal epithelium that is cuboidal and dome-shaped with microvilli. Adjacent cells have elaborate intercellular folds that create dilated intercellular spaces. Read More

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Tangential migration of ameboid microglia in the developing quail retina: mechanism of migration and migratory behavior.

Glia 1998 Jan;22(1):31-52

Departamento de Biología Celular, Facultad de Ciencias, Universidad de Granada, Spain.

Long distance migration of microglial precursors within the central nervous system is essential for microglial colonization of the nervous parenchyma. We studied morphological features of ameboid microglial cells migrating tangentially in the developing quail retina to shed light on the mechanism of migration and migratory behavior of microglial precursors. Many microglial precursors remained attached on retinal sheets containing the inner limiting membrane covered by a carpet of Müller cell endfeet. Read More

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January 1998

Scierai ectasia associated with hereditary retinal dysplasia in a mutant strain of Japanese quail (Coturnix coturnix japonica).

Avian Pathol 1997 ;26(1):53-61

Nippon Institute for Biological Science, Tokyo 198, Japan.

Ocular defects and age-related lesions in mutant (GUB strain) Japanese quail (Coturnix coturnix japonica), phenotypically characterised by silver plumage, are described. Grossly, a circular area of hypopigmentation in the posterior retina with thinning of the subjacent sciera was observed in all GUB quails. As the birds matured, the thinned sciera progressed to scierai ectasia. Read More

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October 2012

Ontogeny of somatostatin immunoreactive neurons in the medial cerebral cortex and other cortical areas of the lizard Podarcis hispanica.

J Comp Neurol 1996 Oct;374(1):118-35

Facultad de Ciencias Biologicas, Universidad de Valencia, Spain.

The ontogeny of somatostatin immunoreactive interneurons in the cerebral cortex of the lizard Podarcis hispanica has been studied in histological series of embryos, perinatal specimens, and adults. Somatostatin immunoreactive interneurons appear in the early stages of lizard cerebral cortex ontogeny, their number increases during embryonary development, reaches a peak in early postnatal life, and decreases in adult lizards. The first somatostatin immunoreactive somata in the lizard forebrain appeared on E36, and they were located in non cortical areas. Read More

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October 1996

Developmental expression of laminin beta 2 in rat retina. Further support for a role in rod morphogenesis.

Invest Ophthalmol Vis Sci 1996 Jul;37(8):1651-61

Department of Biology, Boston College, Chestnut Hill, MA 02167, USA.

Purpose: The authors previously hypothesized that laminin beta 2 (S-laminin) plays a role in directing photoreceptor development. The aim of this study was to examine the temporal and spatial expression pattern of beta 2 laminins in rat retina to test this hypothesis.

Methods: Retinas from Sprague-Dawley rats were harvested on embryonic days (E) 14, 16, and 21, as well as on postnatal days (P) 2, 5, and 10. Read More

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A novel entorhinal projection to the rat dentate gyrus: direct innervation of proximal dendrites and cell bodies of granule cells and GABAergic neurons.

J Neurosci 1996 May;16(10):3322-33

Institute of Anatomy, University of Freiburg, Germany.

Entorhinal fibers to the fascia dentata originating from layer II stellate neurons are known to terminate exclusively in the outer two thirds of the molecular layer, where they innervate distal dendritic portions of dentate neurons. Using anterograde tracing with Phaseolus vulgaris leucoagglutinin, we unraveled a previously unknown entorhinal projection that directly innervates proximal dendritic portions and somata of granule cells and GABAergic neurons. This projection originates from neurons located in entorhinal layers IV-VI of the medial entorhinal area. Read More

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The density and branching of dentinal tubules in human teeth.

I A Mjör I Nordahl

Arch Oral Biol 1996 May;41(5):401-12

College of Dentistry, University of Florida, Gainesville 32610-0415, USA.

Detailed knowledge of dentine structure, and especially that of the dentinal tubules, is essential in order to understand dentine permeability and to interpret data from investigations on dentine adhesive materials. The aim here was to examine the density and branching of dentinal tubules in human teeth by light and scanning electron microscopy. Stained and unstained demineralized sections and undemineralized fractured specimens were studied. Read More

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Wall composition in intravascular ultrasound layered appearance of human coronary artery.

Heart Vessels 1996 ;11(3):152-9

Division of Cardiology, National Cardiovascular Center, Osaka, Japan.

To evaluate the impact of histological factors on the appearance of the wall of the coronary artery by intravascular ultrasound (IVUS), we performed an in vitro study of 34 coronary artery segments from eight autopsied patients. We assumed the coronary cross section to be divided into four equal parts, and assessed the quadrants with maximal and minimal wall thickness by IVUS (30 MHz; 4.3 Fr; 1800 rpm) and by a histological study. Read More

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February 1997

Localization of S-adenosylmethionine decarboxylase in murine tissues by immunohistochemistry.

Eur J Oral Sci 1995 Jun;103(3):133-40

Department of Oral Biochemistry, Faculty of Odontology, Göteborg University, Sweden.

S-adenosylmethionine decarboxylase (AdoMetDC), one of three key enzymes in polyamine biosynthesis, is present in minute concentrations in adult tissues, whereas increased AdoMetDC activity occurs in conjunction with differentiation and growth. The occurrence of AdoMetDC in tissues has earlier been studied by biochemical technique only. In this work, an antiserum against recombinant human AdoMetDC was used to investigate the localization of the enzyme in different mouse tissues. Read More

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