Neuro Oncol 2016 11 3;18(11):1487-1497. Epub 2016 Apr 3.
Molecular Reproduction, Development and Genetics department, Indian Institute of Science, Bangalore, India (S.S.P., P.G., M.B., P.K.); Department of Neuropathology, National Institute of Mental Health and Neuro Sciences, Bangalore, India (S.D.S., J.J., V.S.); Clinical Neurosciences, National Institute of Mental Health and Neuro Sciences, Bangalore, India (A.H.S.); Neurosurgery, National Institute of Mental Health and Neuro Sciences, Bangalore, India (A.A.).
Background: Upregulation of insulin-like growth factor binding protein 2 (IGFBP-2) is often associated with aggressiveness of glioblastoma (GBM) and contributes to poor prognosis for GBM patients. In view of the regulation of β-catenin by IGFBP-2 in breast cancer and the crucial role of β-catenin pathway in glioma invasion, proliferation and maintenance of glioma stem cells, the mechanism of regulation of β-catenin by IGFBP-2, and its role in GBM prognosis was studied.
Methods: Regulation of the β-catenin pathway was studied by immunocytochemistry, Western blot analysis, luciferase assays, and real-time RT-PCR. Read More