61 results match your criteria subcultured consisted


Comparative evaluation of various herbal and synthetic solutions on disinfection of guttapercha: An study.

Indian J Dent Res 2020 May-Jun;31(3):376-381

Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.

Background: Although gutta-percha cones are usually supplied in aseptic packages, once opened and used, they may be contaminated. Chair side disinfection of these cones is important and critical for success of endodontic therapy. Gutta-percha being heat labile, chemical disinfection is the only viable alternative for the sterilization of these cones. Read More

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Development and characterization of a continuous cell line (EL) from the liver of European eel Anguilla anguilla.

Cell Biol Int 2020 Mar 19;44(3):808-820. Epub 2019 Dec 19.

Biotechnology Institute, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou, Fujian, 350003, China.

In the present study, a new hepatic tissue-origin cell line from European eel Anguilla anguilla has been developed and characterized. This cell line designated EL has been maintained in Leibovitz L-15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast-like cells, which could survive over 100 days in vitro, and could grow at 15-32°C. Read More

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Comparative Evaluation of Three Herbal Solutions on the Disinfection of Guttapercha Cones: An In vitro Study.

J Clin Diagn Res 2017 Mar 1;11(3):ZC21-ZC24. Epub 2017 Mar 1.

Postgraduate Student, Department of Conservative Dentistry and Endodontics, Meghna Institute of Dental Sciences, Nizamabad, Telangana, India.

Introduction: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. Read More

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Establishment and characterization of fin-derived cell line from ornamental carp, Cyprinus carpio koi, for virus isolation in India.

In Vitro Cell Dev Biol Anim 2015 Aug 20;51(7):705-13. Epub 2015 May 20.

Peninsular and Marine Fish Genetic Resources Centre, National Bureau of Fish Genetic Resources, CMFRI Campus, P.O. Number 1603, Kochi, Kerala, 682018, India,

Cyprinus carpio koi fin (CCKF) cell line was established and characterized from the caudal fin tissue of ornamental common carp, C. carpio koi. This cell line has been maintained in L-15 medium supplemented with 15% foetal bovine serum (FBS) and subcultured more than 52 times over a period of 24 mo. Read More

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A novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi: development and characterization.

J Fish Biol 2015 Jan 6;86(1):32-45. Epub 2014 Nov 6.

College of Animal Science and Technology, Northwest A&F University, Shanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Guangzhou 510380, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China.

In this study, a continuous cell line (named as CPB) was established from Siniperca chuatsi brain and has been subcultured >140 times. CPB cell line predominantly consisted of fibroblast-like cells that could grow better in Leibovitz's L-15 supplemented with 10% foetal bovine serum at 28° C. Polymerase chain reaction amplification of 18s recombinant (r)RNA confirmed the origin of this cell line from S. Read More

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January 2015

Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses.

Virus Res 2014 Aug 30;189:56-62. Epub 2014 Apr 30.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address:

An Ussuri catfish Pseudobagrus ussuriensis skin (UCS) cell line was developed and subcultured for more than 60 passages. UCS cells consisted of mostly epithelial-like cells and multiplied well in TC199 medium supplemented with 10% fetal bovine serum at 25°C. Chromosome analysis revealed that most UCS cells had a normal diploid karyotype with 2n=52. Read More

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Effects of parathyroid hormone-related peptide on the large conductance calcium-activated potassium channel and calcium homeostasis in vascular smooth muscle cells.

Postgrad Med 2014 Mar;126(2):76-85

Wuppertal Department of Internal Medicine and Angiology, Wuppertal Research and Medical Center, Wuppertal, Germany.

Aim: To demonstrate the impact of the parathyroid hormone-related peptide (PTHrP) on the large conductance calcium-activated potassium (BKCa) channels in vascular smooth muscle cells (VSMC) and hyperpolarization of the cell membrane and its dependence on calcium.

Materials And Methods: VSMC were isolated from rat aorta and further subcultured. Four experiments were conducted in calcium-release measurements and each of them consisted of a control group, PTHrP, chemical substance, and PTHrP + chemical substance. Read More

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First Report of Stem Rot on Cereus peruvianus monstruosus Caused by Bipolaris cactivora (Petr.) Alcorn in Italy.

Plant Dis 2014 Jan;98(1):159

Center of Competence AGROINNOVA, University of Torino, Via Leonardo da Vinci, 44, 10095 Grugliasco, Italy.

Cereus peruvianus monstruosus, known as "monster cactus," family Cactaceae, is grown as a potted plant. In the winter of 2013, a stem rot was observed on a farm located near Ventimiglia (northern Italy) on 80% of 4,000 9-month-old plants grown in trays in a peat substrate. Symptoms consisted of a rapid rot of the upper portion of the stem. Read More

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January 2014

Isolation and characterization of epithelial and myogenic cells by "fishing" for the morphologically distinct cell types in rat primary periodontal ligament cultures.

Differentiation 2013 Feb 4;85(3):91-100. Epub 2013 May 4.

Department of Developmental and Regenerative Dentistry, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan.

The periodontal ligament (PDL) contains various cell populations and plays a central role in the maintenance, repair, and regeneration of the periodontium, i.e., tooth-supporting structures. Read More

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February 2013

Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.

Fish Physiol Biochem 2013 Dec 27;39(6):1399-410. Epub 2013 Mar 27.

Key Laboratory of Agricultural Animal Genetics, College of Fisheries, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

This study established and characterized a new cell line (MAF) from the fin of blunt snout bream (Megalobrama amblycephala), a freshwater fish cultivated in China. MAF cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °C and have been subcultured more than 95 times in almost a year. MAF cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. Read More

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December 2013

First Report of Fusarium oxysporum Causing Wilt on Iceland Poppy (Papaver nudicaule) in Italy.

Plant Dis 2012 Dec;96(12):1823

AGROINNOVA, Università di Torino Via Leonardo da Vinci 44, 10095 Grugliasco, Italy.

During fall 2011, symptoms of a wilt disease were observed in a commercial nursery near Ventimiglia as well as in the Research Center of Floriculture of Sanremo (northern Italy) on plants of Papaver nudicaule (Iceland poppy) of a local unnamed cultivar. In the commercial nursery, 15 to 20% of plants were affected, while about 3% of plants were affected at the Research Center. Symptoms consisted of chlorosis, premature leaf drop, and foliar wilting, followed by the stem wilting, bending, and eventually rotting from the base. Read More

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December 2012

Polysorbate 80 and Helicobacter pylori: a microbiological and ultrastructural study.

BMC Microbiol 2012 Sep 22;12:217. Epub 2012 Sep 22.

Department of Internal Medicine, University of Siena, Siena, Italy.

Background: The frequent occurrence of chemoresistant strains reduces the chances of eradication of H. pylori infection and prompted the investigation of non-antibiotic substances active against this organism. Some surfactants enhance the effectiveness of antibiotics for their permeabilizing properties towards bacteria. Read More

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September 2012

A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).

In Vitro Cell Dev Biol Anim 2012 Aug 30;48(7):426-33. Epub 2012 Jun 30.

Agricultural Research Service, Biological Control of Insects Research Laboratory, USDA, Columbia, MO 65203, USA.

The red flour beetle, Tribolium castaneum, is a model organism for agricultural and medical research and its complete genome is sequenced. We established a continuously replicating T. castaneum cell line to complement existing physiological, genetic, and genomic research tools. Read More

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A new insect cell line from pupal ovary of Spodoptera exigua established by stimulation with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).

In Vitro Cell Dev Biol Anim 2012 May 2;48(5):271-5. Epub 2012 May 2.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

A continuous cell line derived from the pupal ovary of Spodoptera exigua was established by treating primary cells with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Three days after treating cells with 3.0 μg/ml of MNNG, the cells formed a monolayer and were initially subcultured 60 d after the MNNG was removed, followed by subculturing for 30 passages. Read More

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First Report of Leaf Spot of Rocket (Eruca sativa) Caused by Fusarium equiseti in Italy.

Plant Dis 2011 Oct;95(10):1315

Centre of Competence for the Agro-Environmental Sector (AGROINNOVA), Via Leonardo da Vinci 44, 10095 Grugliasco, Italy.

During the summer of 2010, rocket (Eruca sativa) plants grown in an open field and under a plastic tunnel in Piedmont (northern Italy) showed symptoms of a previously reported foliar disease. Symptoms were observed on 30-day-old plants and consisted of small, circular, brown leaf spots (1 to 3 to 10 to 12 mm in diameter), sometimes later becoming elliptical. Necrotic lesions were cracked in the center and showed a well-defined border, frequently surrounded by a violet-brown halo. Read More

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October 2011

First Report of Fusarium oxysporum Causing Wilt on Jade Plant (Crassula ovata) in Italy.

Plant Dis 2011 Sep;95(9):1191

AGROINNOVA, Università di Torino Via Leonardo da Vinci 44, 10095 Grugliasco, Italy.

During summer 2010, symptoms of a wilt disease were observed in a commercial farm in northern Italy on Crassula ovata (jade plant). First symptoms consisted of chlorosis and premature drop of still turgid leaves. As the disease progressed, leaves turned yellow and wilted before dropping off and the stem wilted, bent, and eventually rotted starting from the base. Read More

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September 2011

Goss's Bacterial Blight and Wilt of Corn Caused by Clavibacter michiganensis subsp. nebraskensis Occurs in Minnesota.

Plant Dis 2010 Aug;94(8):1064

Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.

Goss's bacterial wilt and blight caused by Clavibacter michiganensis subsp. nebraskensis (Vidaver and Mandel) Davis et al. can be an economically significant disease of corn (Zea mays L. Read More

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Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790).

In Vitro Cell Dev Biol Anim 2010 Sep 25;46(8):647-56. Epub 2010 Jun 25.

National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, P.O. Number 1603, Kochi, India.

A new cell line [pearlspot fin (PSF)] has been developed from caudal fin of Etroplus suratensis, a brackish/freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum (FBS). The PSF cell line consisted predominantly of epithelial-like cells. Read More

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September 2010

Fusarium Wilt of Strawberry Caused by Fusarium oxysporum in California.

Plant Dis 2009 Oct;93(10):1077

Department of Plant Pathology, University of California, Davis 95616.

Beginning in 2006 and continuing into 2009, an apparently new disease of strawberry (Fragaria × ananassa) affected commercial plantings (cvs. Albion, Camarosa, and others) in coastal (Ventura and Santa Barbara counties) California. Symptoms consisted of wilting of foliage, drying and withering of older leaves, stunting of plants, and reduced fruit production. Read More

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October 2009

Phylogenetic composition and properties of bacteria coexisting with the fungus Hypholoma fasciculare in decaying wood.

ISME J 2009 Oct 11;3(10):1218-21. Epub 2009 Jun 11.

Laboratory of Environmental Microbiology, Institute of Microbiology of the ASCR, v.v.i., Prague 4 14220, Czech Republic.

White-rot fungi are major degraders of woody materials in terrestrial environments because of their ability to decompose lignin. However, little is known on the possible associations of white-rot fungi with other microorganisms during wood decay. We investigated the numbers, community composition and functional traits of bacteria present in natural wood samples under advanced decay by the white-rot basidiomycete Hypholoma fasciculare. Read More

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October 2009

Shunt infection: is there a near-miss scenario?

J Neurosurg 2007 Jan;106(1 Suppl):15-9

Department of Neurosurgery, Great Ormond Street Hospital for Children, NHS Trust, London, United Kingdom.

Object: The aim of this study was to establish whether microbiological contamination at the time of shunt insertion can be detected and used to predict the likelihood of subsequent shunt infection.

Methods: A prospective study of pediatric patients undergoing primary shunt insertion was undertaken. Following the protocol devised for this study, three swab samples were collected from the surgical wounds during each procedure. Read More

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January 2007

Clonal analysis of adult human olfactory neurosphere forming cells.

Biotech Histochem 2005 Sep-Dec;80(5-6):189-200

Department of Anatomical Sciences and Neurobiology, University of Louisville, School of Medicine, Louisville, Kentucky 40292, USA.

Olfactory neuroepithelium (ONe) is unique because it contains progenitor cells capable of mitotic division that replace damaged or lost neurons throughout life. We isolated populations of ONe progenitors from adult cadavers and patients undergoing nasal sinus surgery that were heterogeneous and consisted of neuronal and glial progenitors. Progenitor lines have been obtained from these cultures that continue to divide and form nestin positive neurospheres. Read More

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Development and characterization of a new tropical marine fish cell line from grouper, Epinephelus coioides susceptible to iridovirus and nodavirus.

J Virol Methods 2006 Jan 30;131(1):58-64. Epub 2005 Aug 30.

State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, 135 West Xingang Road, Guangzhou 510275, PR China.

The development and characterization of a new tropical marine fish cell line (GS), derived from the spleen of orange spotted grouper, Epinephelus coioides is described. The GS cells grow well in Leibovitz's L-15 medium supplemented with 10% foetal bovine serum, and have been subcultured more than 200 times. The optimal growth temperature was 27 degrees C. Read More

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January 2006

Long-term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum-free culture system.

Gastroenterology 2005 Jun;128(7):2089-104

Department of Pediatrics, Graduate School of Medicine, Kyoto University, Japan.

Background & Aims: The liver has high regenerative potential. We attempted to establish a novel culture system for extensive expansion of fetal mouse hepatic stem/progenitor cells and to characterize cultured cells.

Methods: Hepatic spheroids collected from 6-day floating cultures were cultured on collagen-coated dishes in serum-free conditions in medium containing growth factors. Read More

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Development of a capture/enrichment sandwich ELISA for the rapid detection of enteropathogenic and enterohaemorrhagic Escherichia coli O26 strains.

J Appl Microbiol 2004 ;97(6):1161-5

Department of Veterinary Sciences, The Queen's University of Belfast, Belfast, UK.

Aims: To improve the sensitivity of a monoclonal antibody (MAb 2F3) based enteropathogenic Escherichia coli (EPEC)/enterohaemorrhagic E. coli (EHEC) serogroup O26-specific sandwich ELISA (sELISA), using a capture/enrichment format of the assay.

Methods And Results: The sELISA utilized an EPEC/EHEC O26-specific MAb 2F3 as the capture reagent and an E. Read More

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Bone tissue engineering on calcium phosphate-coated titanium plates utilizing cultured rat bone marrow cells: a preliminary study.

J Mater Sci Mater Med 1998 Dec;9(12):859-63

University of Twente, The Netherlands.

The use of osteoinductive in vitro tissue-coated implants in orthopaedic and dental surgery (e.g. revision hip arthroplasty), could result in a better fixation of these implants. Read More

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December 1998

Establishment and characterization of two new cell lines derived from flounder, Paralichthys olivaceus (Temminck & Schlegel).

J Fish Dis 2003 Nov-Dec;26(11-12):657-65

Department of Fish Pathology, Yosu National University, Yosu, Chunnam, Republic of Korea.

Two new cell cultures from flounder, Paralichthys olivaceus (Temminck & Schlegel), flounder fin (FFN) cells from fin tissue and flounder spleen (FSP) cells from spleen tissue, were established and characterized. The cells multiplied well in Eagle's minimum essential medium, supplemented with 10% foetal bovine serum, and have been subcultured more than 100 times, becoming continuous cell lines. Modal diploid chromosome number of FFN and FSP cells was 64 and 62, respectively. Read More

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February 2004

Preenrichment versus direct selective agar plating for the detection of Salmonella Enteritidis in shell eggs.

J Food Prot 2003 Sep;66(9):1670-4

US Food and Drug Administration/Center for Food Safety and Applied Nutrition, Office of Plant and Dairy Food and Beverages, 5100 Paint Branch Parkway, College Park, Maryland 20740, USA.

The relative effectiveness of two methods for the recovery of Salmonella Enteritidis (SE) from jumbo and medium shell eggs was compared. The first method used in the comparison consisted of a preenrichment of the sample, and the second method was developed by the U.S. Read More

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September 2003

Establishment of two new cell lines from Bombyx mori (L.) (Lepidoptera: Bombycidae) & their susceptibility to baculoviruses.

Indian J Med Res 2002 May;115:189-93

National Institute of Virology (ICMR), Pune, India.

Background & Objectives: Lepidopteran cell cultures and baculovirus expression vector systems are becoming popular due to their potential applications in biotechnology especially for the expression of foreign proteins. Efforts were made to develop new, indigenous, cell lines from Bombyx mori larvae and pupae.

Methods: Eight to ten B. Read More

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Cultivation and characterization of coronary microvascular endothelial cells: a novel porcine model using micropigs.

Microvasc Res 2002 Sep;64(2):278-88

School of Molecular Biosciences, College of Education, Washington State University, Pullman, 99164, USA.

Coronary microvascular endothelial cells (CMECs) play an important role in many physiological processes. Porcine CMECs from large breed pigs have been isolated and successfully cultured. However, because micropigs offer research advantages over large breed pigs, micropig CMEC (MPCMEC) cultures may be useful as an alternative in vitro porcine model for cardiovascular studies. Read More

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September 2002