30,564 results match your criteria subcellular localization


Nuclear localization of duck Tembusu virus NS5 protein attenuates viral replication in vitro and NS5-NS2B3 interaction.

Vet Microbiol 2021 Sep 20;262:109239. Epub 2021 Sep 20.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang District, Chengdu City, Sichuan Province, 611130, China. Electronic address:

Duck Tembusu virus (TMUV) belongs to the flavivirus genus whose genome replication involved in capping and RNA synthesis dominating by nonstructural protein 5 (NS5). Flaviviral replication has been well documented to occur in the cytoplasm, but the effect of NS5 to gain access to the nucleus remains controversial. Here, TMUV NS5 was observed to localize within the cytoplasm of transfected and infected cells and co-localized with the endoplasmic reticulum. Read More

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September 2021

Transcriptional characterization of subcutaneous adipose tissue in obesity affected women highlights metabolic dysfunction and implications for lncRNAs.

Genomics 2021 Sep 20. Epub 2021 Sep 20.

Department of Biomedical and Clinical Sciences "L. Sacco", University of Milan, Via Grassi 74, 20157 Milan, Italy; Pediatric Clinical Research Centre Fondazione "Romeo ed Enrica Invernizzi", University of Milano, Milano, Italy; Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Milano, Italy. Electronic address:

Obesity is a complex disease with multifactorial causes, and its prevalence is becoming a serious health crisis. For this reason, there is a crucial need to identify novel targets and players. With this aim in mind, we analyzed via RNA-sequencing the subcutaneous adipose tissue of normal weight and obesity-affected women, highlighting the differential expression in the two tissues. Read More

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September 2021

New Insights into Stress-Induced β-Ocimene Biosynthesis in Tea () Leaves during Oolong Tea Processing.

J Agric Food Chem 2021 Sep 23. Epub 2021 Sep 23.

FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

As the major contributors to the floral odors of tea products, terpenoid volatiles play critical roles in the defense response of plants to multiple stresses. Until now, only a few genes in tea plants () have been functionally validated. In this study, by comparative studies conducted at gene, protein, and metabolite levels during oolong tea processing, we isolated an ocimene synthase gene, , which displays a low similarity to previously characterized tea ocimene synthases. Read More

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September 2021

Efficient mesophyll protoplast isolation and development of a transient expression system for castor-oil plant (Ricinus communis L.).

Biol Futur 2019 Jan 16;70(1):8-15. Epub 2019 Nov 16.

Department of Biotechnology, Faculty of Agricultural Science, Guangdong Ocean University, Zhanjiang, Guangdong, P. R. China.

Introduction: We investigated the main factors affecting the efficacy of protoplast isolation, including leaf-obtaining period, cutting shapes of leaf material, enzyme concentration, enzymolysis time, and centrifugal speed.

Methods: Protoplast isolation was optimal on the condition of 20 days of leaf materials, 2-mm filament of leaves, 1.6% RS and 0. Read More

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January 2019

Amyloidosis cutis dyschromica cases caused by GPNMB mutations with different inheritance patterns.

J Dermatol Sci 2021 Aug 10. Epub 2021 Aug 10.

Department of Dermatology, Peking University First Hospital, Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, National Clinical Research Center for Skin and Immune Diseases, Beijing, China. Electronic address:

Background: Amyloidosis cutis dyschromica (ACD) is a rare form of primary cutaneous amyloidosis featured by reticulate dotted hypo- and hyperpigmentation. Recently, loss-of-function mutations in GPNMB, encoding glycoprotein (transmembrane) nonmetastatic melanoma protein B, were found in autosomal-recessive or semi-dominant ACD.

Objective: This study aims to detect the genetic defect underlying ACD in nine separate cases and to investigate the functional consequences of the mutants. Read More

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Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Mol Neurodegener 2021 Sep 22;16(1):66. Epub 2021 Sep 22.

Institute on Aging and Brain Disorders, The First Affiliated Hospital of USTC, Hefei National Laboratory for Physical Sciences at the Microscale, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China.

Background: Aberrant alternative splicing plays critical role in aging and age-related diseases. Heterogeneous nuclear ribonucleoproteins (hnRNPs) reportedly regulate RNA splicing process. Whether and how hnRNPs contribute to age-related neurodegenerative diseases, especially Alzheimer's disease (AD), remain elusive. Read More

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September 2021

RNALocate v2.0: an updated resource for RNA subcellular localization with increased coverage and annotation.

Nucleic Acids Res 2021 Sep 22. Epub 2021 Sep 22.

Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.

Resolving the spatial distribution of the transcriptome at a subcellular level can increase our understanding of biology and diseases. To facilitate studies of biological functions and molecular mechanisms in the transcriptome, we updated RNALocate, a resource for RNA subcellular localization analysis that is freely accessible at http://www.rnalocate. Read More

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September 2021

Nanodissection of Selected Viral Particles by Scanning Transmission Electron Microscopy/Focused Ion Beam for Genetic Identification.

Anal Chem 2021 Sep 22. Epub 2021 Sep 22.

Department of Infectious Diseases, Israel Institute for Biological Research, P.O.B 19, Ness Ziona 74100, Israel.

This study presents the development of a new correlative workflow to bridge the gap between electron microscopy imaging and genetic analysis of viruses. The workflow enables the assignment of genetic information to a specific biological entity by harnessing the nanodissection capability of focused ion beam (FIB). This correlative workflow is based on scanning transmission electron microscopy (STEM) and FIB followed by a polymerase chain reaction (PCR). Read More

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September 2021

Rice ACID PHOSPHATASE 1 regulates Pi stress adaptation by maintaining intracellular Pi homeostasis.

Plant Cell Environ 2021 Sep 22. Epub 2021 Sep 22.

Microelement Research Center, College of Resources & Environment, Huazhong Agricultural University, Wuhan, P. R. China.

The concentration and homeostasis of intracellular phosphate (Pi) are crucial for sustaining cell metabolism and growth. During short-term Pi starvation, intracellular Pi is maintained relatively constant at the expense of vacuolar Pi. After the vacuolar stored Pi is exhausted, the plant cells induce the synthesis of intracellular acid phosphatase (APase) to recycle Pi from expendable organic phosphate (Po). Read More

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September 2021

Subcellular proteomics.

Nat Rev Methods Primers 2021 29;1. Epub 2021 Apr 29.

Department of Biochemistry, University of Cambridge, Cambridge, UK.

The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchrony. Dynamic movement of proteins between niches is essential for cellular processes such as signalling, growth, proliferation, motility and programmed cell death, and mutations causing aberrant protein localization are associated with a wide range of diseases. Read More

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Expression and subcellular localization of Discoidin Domain Receptor 1 (DDR1) define prostate cancer aggressiveness.

Cancer Cell Int 2021 Sep 21;21(1):507. Epub 2021 Sep 21.

Department of Pathology, Wayne State University School of Medicine and Karmanos Cancer Institute, Scott Hall #8200, 540 E. Canfield St, Detroit, MI, 48201, USA.

Background: The Discoidin Domain Receptor 1 (DDR1) is one of the two members of a unique family of receptor tyrosine kinase receptors that signal in response to collagen, which has been implicated in cancer progression. Here, we examined the expression of DDR1 in prostate cancer (PCa), and assessed its potential value as a prognostic marker, as a function of grade, stage and other clinicopathologic parameters.

Methods: We investigated the association between the expression level and subcellular localization of DDR1 protein and PCa aggressiveness by immunohistochemistry, using tissue microarrays (TMAs) encompassing 200 cases of PCa with various Gleason scores (GS) and pathologic stages with matched normal tissue, and a highly specific monoclonal antibody. Read More

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September 2021

Endosomal disentanglement of a transducible artificial transcription factor targeting endothelin receptor A.

Mol Ther 2021 Sep 18. Epub 2021 Sep 18.

Department of Biomedicine, University Basel, 4031 Basel, Switzerland; Department of Ophthalmology, University Basel, 4031 Basel, Switzerland. Electronic address:

Cell-penetrating peptides (CPPs) hold great promise for intracellular delivery of therapeutic proteins. However, endosomal entrapment of transduced cargo is a major bottleneck hampering their successful application. While developing a transducible zinc finger protein-based artificial transcription factor targeting the expression of endothelin receptor A, we identified interaction between the CPP and the endosomal membrane or endosomal entanglement as a main culprit for endosomal entrapment. Read More

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September 2021

Far from the nuclear crowd: Cytoplasmic lncRNA and their implications in synaptic plasticity and memory.

Neurobiol Learn Mem 2021 Sep 18:107522. Epub 2021 Sep 18.

National Brain Research Centre, NH-8, Nainwal Mode, Manesar 122052, Haryana, India. Electronic address:

A striking proportion of long non-coding RNAs are expressed specifically in the mammalian brain. Advances in genome-wide sequencing detected widespread diversity in neuronal lncRNAs based on their expression pattern, localization and function. A growing body of literature proposes that localization of lncRNAs is a critical determinant of their function. Read More

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September 2021

LncRNA SCIRT absorbs miR-221 to advance the expression of lncRNA GAS5 in oral squamous cell carcinoma to inhibit cancer cell apoptosis.

J Oral Pathol Med 2021 Sep 21. Epub 2021 Sep 21.

Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, P. R. China.

Background: Although SCIRT has been reported to suppress breast cancer, its role in other cancers, such as oral squamous cell carcinoma (OSCC), is hardly known. We predicted that SCIRT might interact with miR-221 to target lncRNA GAS5 and analyzed the interaction between SCIRT and miR-221 in OSCC.

Methods: SCIRT and miR-221 expression levels were quantified using RT-qPCR. Read More

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September 2021

Disease mechanisms of X-linked cone dystrophy caused by missense mutations in the red and green cone opsins.

FASEB J 2021 Oct;35(10):e21927

Department of Ophthalmology, University of Florida, Gainesville, Florida, USA.

Cone photoreceptors are responsible for the visual acuity and color vision of the human eye. Red/green cone opsin missense mutations N94K, W177R, P307L, R330Q, and G338E have been identified in subjects with congenital blue cone monochromacy or color-vision deficiency. Studies on disease mechanisms due to these cone opsin mutations have been previously carried out exclusively in vitro, and the reported impairments were not always consistent. Read More

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October 2021

Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase.

Bioengineered 2021 Dec;12(1):7119-7130

Department of Otolaryngology & Head and Neck, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, Nanning City, P.R. China.

Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect LINC02570 expression in nasopharyngeal cell lines, NPC tissues, and chronic rhinitis tissues. Read More

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December 2021

Discovery of New 4-Indolyl Quinazoline Derivatives as Highly Potent and Orally Bioavailable P-Glycoprotein Inhibitors.

J Med Chem 2021 Sep 21. Epub 2021 Sep 21.

School of Pharmaceutical Sciences & Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou 450001, China.

The major drawbacks of P-glycoprotein (P-gp) inhibitors at the clinical stage make the development of new P-gp inhibitors challenging and desirable. In this study, we reported our structure-activity relationship studies of 4-indolyl quinazoline, which led to the discovery of a highly effective and orally active P-gp inhibitor, . effectively reversed multidrug resistance (MDR) to paclitaxel and colchicine in SW620/AD300 and HEK293T-ABCB1 cells. Read More

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September 2021

Incorporating label correlations into deep neural networks to classify protein subcellular location patterns in immunohistochemistry images.

Proteins 2021 Sep 21. Epub 2021 Sep 21.

Institute of Image Processing and Pattern Recognition, Shanghai Jiao Tong University, and Key Laboratory of System Control and Information Processing, Ministry of Education of China, Shanghai, China.

Analysis of protein subcellular localization is a critical part of proteomics. In recent years, as both the number and quality of microscopic images are increasing rapidly, many automated methods, especially convolutional neural networks, have been developed to predict protein subcellular location(s) based on bioimages, but their performance always suffers from some inherent properties of the problem. First, many microscopic images have non-informative or noisy sections, like unstained stroma and unspecific background, which affect the extraction of protein expression information. Read More

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September 2021

Identification and validation of an ECERIFERUM2- LIKE gene controlling cuticular wax biosynthesis in cabbage (Brassica oleracea L. var. capitata L.).

Theor Appl Genet 2021 Sep 21. Epub 2021 Sep 21.

Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 ZhongGuanCun South St., Beijing, 100081, China.

Key Message: A single nucleotide mutation of BoCER2 is the primary cause of the wax deficiency in cabbage. An effective allele-specific KASP marker was developed for marker-assisted selection of glossiness. TL28-1 is a novel spontaneous wax-deficient mutant with a glossy phenotype identified from cabbage. Read More

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September 2021

Augmented sequence features and subcellular localization for functional characterization of unknown protein sequences.

Med Biol Eng Comput 2021 Sep 20. Epub 2021 Sep 20.

Department of Computer Science & Engineering, National Institute of Technology Raipur, GE Road, Raipur, Chhattisgarh, 492010, India.

Advances in high-throughput techniques lead to evolving a large number of unknown protein sequences (UPS). Functional characterization of UPS is significant for the investigation of disease symptoms and drug repositioning. Protein subcellular localization is imperative for the functional characterization of protein sequences. Read More

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September 2021

Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana.

Authors:
Cristina Ruberti

Methods Mol Biol 2022 ;2363:153-163

Department of Biology, University of Padua, Padua, Italy.

Transient transformation assays for the analysis of protein localization are routinely used as rapid and convenient alternatives to stable transformation. In this chapter, we describe two transient gene expression assays (e.g. Read More

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January 2022

Surface charge manipulation and electrostatic immobilization of synaptosomes for super-resolution imaging: a study on tau compartmentalization.

Sci Rep 2021 Sep 20;11(1):18583. Epub 2021 Sep 20.

Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.

Synaptosomes are subcellular fractions prepared from brain tissues that are enriched in synaptic terminals, widely used for the study of neural transmission and synaptic dysfunction. Immunofluorescence imaging is increasingly applied to synaptosomes to investigate protein localization. However, conventional methods for imaging synaptosomes over glass coverslips suffer from formaldehyde-induced aggregation. Read More

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September 2021

Genome-wide identification and analysis of WRKY gene family in maize provide insights into regulatory network in response to abiotic stresses.

BMC Plant Biol 2021 Sep 20;21(1):427. Epub 2021 Sep 20.

Anhui Provincial Key Lab. of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu, 241000, China.

Background: The WRKY transcription factor family plays significant roles in biotic and abiotic stress responses, which has been associated with various biological processes in higher plants. However, very little is known regarding the structure and function of WRKY genes in maize.

Results: In this study, a total of 140 ZmWRKY proteins encoded by 125 ZmWRKY genes were eventually identified in maize. Read More

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September 2021

RNase Sensitivity Screening for Nuclear Bodies with RNA Scaffolds in Mammalian Cells.

Bio Protoc 2017 Apr 20;7(8):e2232. Epub 2017 Apr 20.

Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.

The mammalian cell nucleus is highly organized and contains membraneless nuclear bodies (NBs) characterized by distinct resident factors. The NBs are thought to serve as sites for biogenesis and storage of certain RNA and protein factors as well as assembly of ribonucleoprotein complexes. Some NBs are formed with architectural RNAs (arcRNAs) as their structural scaffolds and additional NBs likely remain unidentified in mammalian cells. Read More

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Protein Localization in the Cyanobacterium sp. PCC7120 Using Immunofluorescence Labeling.

Bio Protoc 2017 Jun 5;7(11):e2318. Epub 2017 Jun 5.

Laboratorio de Ecología Microbiana y Toxicología Ambiental, Department of Molecular Genetics and Microbiology, Pontificia Universidad Católica de Chile, Santiago, Chile.

Techniques such as immunoflorescence are widely used to determine subcellular distribution of proteins. Here we report on a method to immunolocalize proteins in sp. PCC7120 with fluorophore-conjugated antibodies by fluorescence microscopy. Read More

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Dysregulation in Subcellular Localization of Myelin Basic Protein mRNA Does Not Result in Altered Myelination in Amyotrophic Lateral Sclerosis.

Front Neurosci 2021 1;15:705306. Epub 2021 Sep 1.

Euan MacDonald Centre for MND Research, The University of Edinburgh, Edinburgh, United Kingdom.

Pathological hallmarks of amyotrophic lateral sclerosis (ALS), including protein misfolding, are well established in oligodendrocytes. More recently, an RNA trafficking deficit of key myelin proteins has been suggested in oligodendrocytes in ALS but the extent to which this affects myelination and the relative contribution of this to disease pathogenesis is unclear. ALS autopsy research findings showing demyelination contrasts with the routine clinical-pathological workup of ALS cases where it is rare to see white matter abnormalities other than simple Wallerian degeneration secondary to widespread neuronal loss. Read More

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September 2021

The MADS-box gene PpPI is a key regulator of the double-flower trait in peach.

Physiol Plant 2021 Sep 19. Epub 2021 Sep 19.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China.

Double flower is an invaluable trait in ornamental peach, but the mechanism underlying its development remains largely unknown. Here, we report the roles of ABCE model genes in double flower development in peach. A total of nine ABCE regulatory genes, including eight MADS-box genes and one AP2/EREBP gene, were identified in the peach genome. Read More

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September 2021

Mesenchymal Stem Cell-Derived Exosome-Containing Linc00632 Regulates GATA Binding Protein-3 Expression in Allergic Rhinitis by Interacting with Enhancer of Zeste Homolog 2 to Inhibit T Helper Cell 2 Differentiation.

Int Arch Allergy Immunol 2021 Sep 17:1-11. Epub 2021 Sep 17.

Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

Background: Allergic rhinitis (AR) is regarded as one of the most common allergic disease of nasal mucosa affecting many people worldwide. Long noncoding RNAs are critical modulators affecting AR progression, whereas the pathogenesis of Linc00632 in the development of AR remains unclear.

Methods: T helper cell 2 (Th2) differentiation of CD4+ T cells was measured by flow cytometry. Read More

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September 2021

Regulatory functions of ROS dynamics via glutathione metabolism and glutathione peroxidase activity in developing rice zygote.

Plant J 2021 Sep 19. Epub 2021 Sep 19.

Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa, Hachioji, Tokyo, Japan.

Reactive oxygen species (ROS) play essential roles in plant development and environmental stress responses. In this study, ROS dynamics, glutathione redox status, expression and subcellular localization of glutathione peroxidases (GPXs) and effect of inhibitors of ROS-mediated metabolism were investigated along with fertilization and early zygotic embryogenesis in rice. Zygotes and early embryos exhibited developmental arrest upon inhibition of ROS production. Read More

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September 2021

Eya1 protein distribution during embryonic development of Xenopus laevis.

Gene Expr Patterns 2021 Sep 15;42:119213. Epub 2021 Sep 15.

School of Natural Sciences, National University of Galway, Galway, Ireland. Electronic address:

Eya1 and other Eya proteins are important regulators of progenitor proliferation, cell differentiation and morphogenesis in all three germ layers. At present, most of our knowledge of Eya1 distribution is based on in situ hybridization for Eya1 mRNA. However, to begin to dissect the mechanisms underlying Eya1 functions, we need a better understanding of the spatiotemporal distribution of Eya1 proteins during embryonic development, their subcellular localization and their levels of expression in various tissues. Read More

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September 2021