67 results match your criteria study 48 months

The clinicopathologic spectrum and genomic landscape of de-/trans-differentiated melanoma.

Mod Pathol 2021 Jun 21. Epub 2021 Jun 21.

Experimental Cancer Genetics, Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

Dedifferentiation and transdifferentiation are rare and only poorly understood phenomena in cutaneous melanoma. To study this disease more comprehensively we have retrieved 11 primary cutaneous melanomas from our pathology archives showing biphasic features characterized by a conventional melanoma and additional areas of de-/trans-differentiation as defined by a lack of immunohistochemical expression of all conventional melanocytic markers (S-100 protein, SOX10, Melan-A, and HMB-45). The clinical, histologic, and immunohistochemical findings were recorded and follow-up was obtained. Read More

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Molecular Basis of Resistance to First-Line Drugs of Strains in Greece.

Microb Drug Resist 2021 Apr 20. Epub 2021 Apr 20.

Department of Microbiology, University Hospital of Larissa, Larissa, Greece.

The aim of this study was to determine the rate and the mutations of genes involved to the first-line antituberculous drugs' resistance of isolated in Central Greece from 2010 to 2019. During the study period, the rate of resistance to isoniazid, rifampicin, ethambutol, and pyrazinamide was 5.4%, 0. Read More

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Electron ionization gas chromatography-mass spectrometry (ei-gc-ms) analysis of extracted oil from Tribulus terrestris seeds.

Pak J Pharm Sci 2020 Nov;33(6):2633-2641

Baqai Institute of Pharmaceutical Sciences, Karachi, Pakistan.

The fine powdered form T. terrestris seeds, was extracted with n-hexane by soxhlet apparatus. The aim of the study was to analyze the T. Read More

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November 2020

Short-term effect of shock ammonium nitrogen load on activated sludge properties.

Water Sci Technol 2019 Dec;80(11):2191-2199

Faculty of Civil Engineering, Research Center for Civil Engineering, Water Research Laboratory, Riga Technical University, Paula Valdena Street 1 - 204, Riga LV-1048, Latvia E-mail:

The activated sludge process suffers from rapid load changes of ammonium (NH), which may result in process failure during wastewater treatment. In this study, the response of activated sludge properties in terms of microfauna composition and sludge volume index (SVI and SVI) on short-term increase of NH concentration (from 55 mg/l to 105 mg/l) was evaluated in batch scale reactors over 72 h. The results show that the first-step nitrification (NH transformation to nitrite (NO)) was inhibited after 24 h, whereas the second-step nitrification (NO transformation to nitrate (NO)) was not significantly affected. Read More

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December 2019

Giardia is the most prevalent parasitic infection in dogs and cats with diarrhea in the city of Medellín, Colombia.

Vet Parasitol Reg Stud Reports 2019 12 11;18:100335. Epub 2019 Sep 11.

CIBAV Research Group, Faculty of Agrarian Science, University of Antioquia, Carrera 75 No 65-87 Bloque 47-241, Medellín, Colombia. Electronic address:

Parasitic agents are a common cause of diarrhea in dogs and cats and, thus, determining their prevalence is essential to establish preventive and control measures. This retrospective study examined the fecal tests records from 1111 dogs and 203 cats with diarrhea submitted to a diagnostic laboratory in the city of Medellin between January and May 2018. The detection of parasites was carried out by direct smears and simple flotation methods. Read More

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December 2019

Long-term results of an intensive cognitive behavioral pain management program for patients with chronic low back pain: a concise report of an extended cohort with a minimum of 5-year follow-up.

Eur Spine J 2019 Jul 8;28(7):1579-1585. Epub 2019 May 8.

Department of Orthopedics, Sint Maartenskliniek, P.O. Box 9011, 6500 GM, Nijmegen, The Netherlands.

Purpose: Treatment options for chronic low back pain (CLBP) include cognitive behavioral interventions. Most of these interventions only have small and short-lived effects. Using strict inclusion criteria for participation in an intensive combined physical and psychological program, encouraging effects were reported at 1-year follow-up. Read More

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Crystal structure of Plasmodium falciparum adenosine deaminase reveals a novel binding pocket for inosine.

Arch Biochem Biophys 2019 05 16;667:6-13. Epub 2019 Apr 16.

National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand. Electronic address:

Plasmodium falciparum (Pf), a malarial pathogen, can only synthesize purine nucleotides employing a salvage pathway because it lacks de novo biosynthesis. Adenosine deaminase (ADA), one of the three purine salvage enzymes, catalyzes the irreversible hydrolytic deamination of adenosine to inosine, which is further converted to GMP and AMP for DNA/RNA production. In addition to adenosine conversion, Plasmodium ADA also catalyzes the conversion of 5'-methylthioadenosine, derived from polyamine biosynthesis, into 5'-methylthioinosine whereas the human enzyme is not capable of this function. Read More

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OXA-48-producing Enterobacterales in different ecological niches in Algeria: clonal expansion, plasmid characteristics and virulence traits.

J Antimicrob Chemother 2019 07;74(7):1848-1855

Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, Nîmes, France.

Objectives: To investigate the prevalence and molecular characteristics of OXA-48-carbapenemase-producing Enterobacterales strains recovered from various ecological niches in Algeria.

Methods: In total, 3309 samples were collected from different ecological niches (human carriage, animal farms, wild animals, pets, food products, aquatic environment and wastewater treatment plants) distributed among six provinces in Algeria between December 2015 and April 2017. The potential presence of OXA-48-producing Enterobacterales isolates was screened on selective medium. Read More

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Three-dimensional tumor visualization of invasive breast carcinomas using whole-mount serial section histopathology: implications for tumor size assessment.

Breast Cancer Res Treat 2019 Apr 5;174(3):669-677. Epub 2019 Jan 5.

Departments of Medical Biophysics and Medical Imaging, Faculty of Medicine, University of Toronto, Toronto, Canada.

Purpose: Linear tumor size (T-size) estimated with conventional histology informs breast cancer management. Previously we demonstrated significant differences in margin and focality estimates using conventional histology versus digital whole-mount serial sections (WMSS). Using WMSS we can measure T-size or volume. Read More

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First report on the nationwide incidence of type 1 diabetes and ketoacidosis at onset in children in Serbia: a multicenter study.

Eur J Pediatr 2018 Aug 17;177(8):1155-1162. Epub 2018 May 17.

Institute for Medical Statistics and Informatics, School of Medicine, University of Belgrade, Dr Subotica 15, Belgrade, 11000, Serbia.

Data regarding incidence of type 1 diabetes (T1DM), as well as data on frequency and severity of diabetic ketoacidosis (DKA) at the time of T1DM diagnosis is of paramount importance for national and regional healthcare planning. The aim of present multicenter study was to provide the first report regarding nationwide annual incidence rates for T1DM in youth in Serbia, as well as prevalence of DKA at the time of diagnosis. Data on all pediatric patients with newly diagnosed T1DM was retrospectively collected from all 15 regional centers for pediatric diabetes in Serbia during the period 2007-2017. Read More

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Spread of Klebsiella pneumoniae ST395 non-susceptible to carbapenems and resistant to fluoroquinolones in North-Eastern France.

J Glob Antimicrob Resist 2018 06 4;13:98-103. Epub 2017 Nov 4.

CHU Reims, Hôpital Robert Debré, Laboratoire de Bactériologie-Virologie-Hygiène, 51092 Reims, France; UFR Médecine, SFR CAP-Santé, EA 4687, Université de Reims Champagne-Ardenne, 51092 Reims, France.

Objectives: Fluoroquinolones (FQs) are a potential treatment for infections caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae that are susceptible to these agents.

Methods: Owing to increasing non-susceptibility to carbapenems among Enterobacteriaceae, in this study FQ resistance mechanisms were characterised in 36 ertapenem-non-susceptible Klebsiella pneumoniae isolated from North-Eastern France in 2012. The population structure was described by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Read More

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A Long-Term Study of the Diversity of OXA-48-Like Carbapenemase-Producing Bacterial Strains in Infected Patients and Carriers.

Microb Drug Resist 2018 Mar 14;24(2):181-189. Epub 2017 Jul 14.

3 Laboratory of Bacteriology, Saint-Antoine Hospital , Assistance Publique-Hôpitaux de Paris, Paris, France .

Purpose: Since their emergence at the beginning of the century, OXA-48 carbapenemases have spread in the community and in hospitals. To assess the diversity of OXA-48-producing bacterial strains and plasmids in the hospital setting, we studied the strains isolated from patients in three hospitals in the Paris area.

Materials And Methods: All possible OXA-48-like strains were included in the study. Read More

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A meta-regression analysis of 41 Australian problem gambling prevalence estimates and their relationship to total spending on electronic gaming machines.

BMC Public Health 2017 05 23;17(1):495. Epub 2017 May 23.

School of Business and Tourism, Southern Cross University, Hogbin Drive, Coffs Harbour, NSW, 2450, Australia.

Background: Many jurisdictions regularly conduct surveys to estimate the prevalence of problem gambling in their adult populations. However, the comparison of such estimates is problematic due to methodological variations between studies. Total consumption theory suggests that an association between mean electronic gaming machine (EGM) and casino gambling losses and problem gambling prevalence estimates may exist. Read More

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Improving spatial microsimulation estimates of health outcomes by including geographic indicators of health behaviour: The example of problem gambling.

Health Place 2017 07 29;46:29-36. Epub 2017 Apr 29.

Fenner School of Environment and Society, The Australian National University, 48A Linnaeus Way, Canberra, ACT 2601, Australia. Electronic address:

Gambling is an important public health issue, with recent estimates ranking it as the third largest contributor of disability adjusted life years lost to ill-health. However, no studies to date have estimated the spatial distribution of gambling-related harm in small areas on the basis of surveys of problem gambling. This study extends spatial microsimulation approaches to include a spatially-referenced measure of health behaviour as a constraint variable in order to better estimate the spatial distribution of problem gambling. Read More

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Encouraging employees to report verbal violence in primary health care in Serbia: A cross-sectional study.

Zdr Varst 2017 Mar 28;56(1):11-17. Epub 2016 Jul 28.

Primary Health Center New Belgrade, Djordja Cutukovica 48a, Zemun, 11070 Belgrade, Serbia.

Introduction: Workplace violence is a serious and multidimensional problem that adversely affects professional and personal lives of employees. The aim of this study was to assess the prevalence and characteristics of verbal violence as a part of psychological violence among employees in primary health care in Belgrade, and to identify contributing factors of verbal violence in the workplace.

Methods: In this cross-sectional study, the final analysis included 1526 employees, using multi-stage sampling. Read More

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OXA-48-like carbapenemases in the UK: an analysis of isolates and cases from 2007 to 2014.

J Antimicrob Chemother 2017 05;72(5):1340-1349

Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London NW9 5EQ, UK.

Objectives: OXA-48-like carbapenemases have spread worldwide since 2001. We analysed patient and microbiological data for UK isolates with these enzymes as confirmed by the national reference laboratory from November 2007 to December 2014.

Methods: MICs were determined using BSAC agar dilution. Read More

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Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study.

Biochim Biophys Acta 2016 11 7;1858(11):2911-2922. Epub 2016 Sep 7.

Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany; Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg, 06120 Halle/Saale, Germany. Electronic address:

The outermost layer of the mammalian skin, the stratum corneum (SC), is a very thin structure and realizes simultaneously the main barrier properties. The penetration barrier for xenobiotica is mostly represented by a complex lipid matrix. There is great interest in the subject of getting information about the arrangement of the lipids, which are mainly ceramides (CER), free fatty acids (FFA) and cholesterol (CHOL). Read More

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November 2016

Bioequivalence of Alendronate and Vitamin D3 in a Combination Tablet Versus Corresponding-Dose Individual Tablets in Healthy Taiwanese Volunteers, Determined Using a Novel Plasma Alendronate Assay.

Curr Ther Res Clin Exp 2015 Dec 14;77:116-21. Epub 2015 Oct 14.

Merck Research Laboratories, Merck & Co., Inc., Kenilworth, New Jersey.

Objective: This study was designed to demonstrate that alendronate (ALN)/vitamin D3 combination tablets (ALN/D5600) are bioequivalent to corresponding doses of ALN and vitamin D3 as individual tablets in healthy Taiwanese volunteers.

Methods: In this open-label, randomized, 2-period, crossover study, 68 volunteers were randomized to a single ALN/D5600 combination tablet or corresponding doses of 70 mg ALN + 5600 IU vitamin D3 (2 × 2800 IU), followed by a 12-day washout period and administration of the alternate formulation. Plasma ALN levels were measured using a newly developed assay. Read More

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December 2015

DRD1 and DRD4 dopamine receptors in the etiology of preeclampsia.

Ginekol Pol 2015 Sep;86(9):672-7

Introduction: Recent reports have suggested an association between genetic polymorphisms of dopamine receptors and the development of an increased risk of chronic hypertension, as well as preeclampsia (PE).

Objectives: The aim of the study was to evaluate the impact of the -48A>G DRD1 and -521C>T DRD4 polymorphisms in the etiology of PE among Polish women.

Material And Methods: Ninety-eight preeclamptic women and 120 healthy pregnant controls were enrolled in the study The investigated polymorphisms of the DRD 1 and DRD4 genes were identified using PCR/RFLP methods. Read More

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September 2015

Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France.

Front Microbiol 2015 23;6:1186. Epub 2015 Oct 23.

Laboratoire de Bactériologie-Virologie-Hygiène, Hôpital Robert Debré, CHU Reims Reims, France ; Structure Fédérative de Recherche CAP-Santé, UFR Médecine, Université de Reims Champagne-Ardenne Reims, France.

Fluoroquinolone (FQ) agents are a potential resort to treat infection due to Enterobacteriaceae producing extended spectrum β-lactamase and susceptible to FQ. In a context of increase of non-susceptibility to carbapenems among Enterobacteriaceae, we characterized FQ resistance mechanisms in 75 Enterobacter cloacae isolates non-susceptible to ertapenem in North-Eastern France in 2012 and describe the population structure by pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Among them, 14. Read More

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November 2015

Pain, instability, and familial discord: a qualitative study into women who use drugs in Malaysia.

Harm Reduct J 2015 Nov 5;12:52. Epub 2015 Nov 5.

Centre for Drug Research, Universiti Sains Malaysia, 11800, Penang, Malaysia.

Background: Out of 20,887 persons who use drugs that came into contact with the National Anti-Drugs Agency (NADA) officials in the year 2013, 3.2% were women. Because women who use drugs (WWUD) are often a hidden population, this may be an underestimate. Read More

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November 2015

A single-day point-prevalence study of faecal carriers in long-term care hospitals in Madrid (Spain) depicts a complex clonal and polyclonal dissemination of carbapenemase-producing Enterobacteriaceae.

J Antimicrob Chemother 2016 Feb 30;71(2):348-52. Epub 2015 Oct 30.

Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain

Objectives: The objective of this study was to describe the prevalence and microbiological characteristics of carbapenemase-producing Enterobacteriaceae (CPE) colonizing patients in long-term care hospitals (LTCHs) in Madrid, Spain.

Methods: Three LTCHs were included in a single-day point-prevalence survey (September 2013). Rectal swabs, collected from all hospitalized patients (137 in LTCH-A, 121 in LTCH-B and 83 in LTCH-C), were plated onto chromogenic media. Read More

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February 2016

Comparison of weight and length at birth of non-Roma and Roma newborn in Serbia.

Int J Public Health 2016 Jan 5;61(1):69-73. Epub 2015 Sep 5.

Institute for Children and Youth, Novi Sad, Vojvodina, Serbia.

Objectives: Roma infants tend to be smaller and are diagnosed as SGA more often than non-Roma infants, suggesting that specific anthropometric norms for these infants may be useful. We aimed to construct population-based centile, gender-specific charts for birth weight and length for singleton Roma infants born from 35 to 42 weeks of gestation and to compare it with anthropometric data of non-Roma infants.

Methods: We analyzed data on 27,602 non-Roma (53 % males) and 2235 Roma (51 % males) singleton live infants delivered from 2006 to 2012 in South East Serbia. Read More

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January 2016

Modification of fatty acid profile of cow milk by calcium salts of fatty acids and its use in ice cream.

J Food Sci Technol 2015 Feb 18;52(2):1061-7. Epub 2013 Jun 18.

Department of Dairy Technology, University of Veterinary and Animal Sciences, Sheikh Abdul Qadir Jilani Road Lower Mall, Lahore, Pakistan.

This study was conducted to determine the effect of calcium salts of fatty acids (CSFA) on fatty acid profile of milk of "Sahiwal" cows and suitability of milk with modified fatty acids in the formulation of ice cream. Fatty acid profile of cow milk was modified by feeding CSFA to eighteen randomly stratified "Sahiwal" cows of first and early lactation divided into three groups. CSFA were offered at two different levels i. Read More

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February 2015

Molecular docking and dynamics simulation study on the influence of Zn2+ on the binding modes of aggrecanase with its inhibitors.

Comb Chem High Throughput Screen 2014 ;17(10):891-903

Department of Bioinformatics, SRM University, SRM Nagar, Kattankulathur, Kancheepuram District, Chennai 603203, India

Zinc plays a vital role in structural organization, regulation of function and stabilization of the folded protein, which ultimately activates or inactivates the binding sites of the protein. Its transition makes a major change in the protein and its binding affinity. The ligand binding aggrecanases can be influenced by Zn2+ ions; therefore the study focuses on checking the binding mode in the presence and absence of zinc using Docking and Molecular dynamics simulation. Read More

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September 2015

Treatment outcome following use of the erbium, chromium:yttrium, scandium, gallium, garnet laser in the non-surgical management of peri-implantitis: a case series.

Br Dent J 2014 Oct;217(8):453-7

Dept of Periodontology, Kings College London Dental Institute, Floor 21, Tower Wing Guys Hospital, London, SE1 9RT.

Aim: To date there is no consensus on the appropriate usage of lasers in the management of peri-implantitis. Our aim was to conduct a retrospective clinical analysis of a case series of implants treated using an erbium, chromium:yttrium, scandium, gallium, garnet laser.

Materials And Methods: Twenty-eight implants with peri-implantitis in 11 patients were treated with an Er,Cr:YSGG laser (68 sites >4 mm), using a 14 mm, 500 μm diameter, 60° (85%) radial firing tip (1. Read More

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October 2014

Genetic evolution of T-cell resistance in the course of melanoma progression.

Clin Cancer Res 2014 Dec 7;20(24):6593-604. Epub 2014 Oct 7.

Department of Dermatology, University Hospital Essen, University Duisburg-Essen, Essen, Germany. German Cancer Consortium (DKTK), Germany.

Purpose: CD8(+) T lymphocytes can kill autologous melanoma cells, but their activity is impaired when poorly immunogenic tumor phenotypes evolve in the course of disease progression. Here, we analyzed three consecutive melanoma lesions obtained within one year of developing stage IV disease for their recognition by autologous T cells.

Experimental Design: One skin (Ma-Mel-48a) and two lymph node (Ma-Mel-48b, Ma-Mel-48c) metastases were analyzed for T-cell infiltration. Read More

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December 2014

Carbapenemase and virulence factors of Enterobacteriaceae in North Lebanon between 2008 and 2012: evolution via endemic spread of OXA-48.

J Antimicrob Chemother 2014 Oct 5;69(10):2699-705. Epub 2014 Jun 5.

Clermont Université, UMR 1071 Inserm/Université d'Auvergne, 63000 Clermont-Ferrand, France INRA, USC 2018, 63000 Clermont-Ferrand, France Centre Hospitalier Universitaire, 63000 Clermont-Ferrand, France

Objectives: To investigate the resistance to carbapenems in Enterobacteriaceae and the underlying resistance mechanisms in North Lebanon between 2008 and 2012.

Methods: A total of 2767 Enterobacteriaceae isolates recovered from clinical samples collected in Nini Hospital (North Lebanon) were screened for a decrease in susceptibility or resistance to ertapenem (MIC >0.25 mg/L). Read More

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October 2014

Analysis of the promoters involved in enterocin AS-48 expression.

PLoS One 2014 4;9(3):e90603. Epub 2014 Mar 4.

Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.

The enterocin AS-48 is the best characterized antibacterial circular protein in prokaryotes. It is a hydrophobic and cationic bacteriocin, which is ribosomally synthesized by enterococcal cells and post-translationally cyclized by a head-to-tail peptide bond. The production of and immunity towards AS-48 depend upon the coordinated expression of ten genes organized in two operons, as-48ABC (where genes encoding enzymes with processing, secretion, and immunity functions are adjacent to the structural as-48A gene) and as-48C1DD1EFGH. Read More

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November 2014

Molecular analysis of OXA-48-carrying conjugative IncL/M-like plasmids in clinical isolates of Klebsiella pneumoniae in Ireland.

Microb Drug Resist 2014 Aug 12;20(4):270-4. Epub 2014 Feb 12.

1 UCD Centre for Molecular Innovation & Drug Discovery, School of Public Health, Physiotherapy & Population Science, University College Dublin , Dublin, Ireland .

This study characterized an IncL/M-like plasmid containing a bla(OXA-48)-encoding gene from a clinical isolate of Klebsiella pneumoniae, denoted as E71T. Investigation of this plasmid sequence identified unique regions of interest along with conserved regions detected in eight other clinical carbapenem-resistant isolates. A 63-kb plasmid (pE71T) from K. Read More

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