327,899 results match your criteria studies murine

Modification of 5-methylphenanthridium from benzothiazoles to indoles as potent FtsZ inhibitors: Broadening the antibacterial spectrum toward vancomycin-resistant enterococci.

Eur J Med Chem 2021 Jul 26;224:113723. Epub 2021 Jul 26.

Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 West Culture Road, Jinan, 250012, China. Electronic address:

The death caused by pathogenic bacteria has always been a severe threat to mankind. The prevalence of drug resistance among bacteria underscores an urgent goal for new antibacterial agents with novel mode of action. Here we first designed and synthesized a class of benzothiazolyl-5-methylphenanthridium derivatives and evaluated their antibacterial activity. Read More

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Dioscin prevents DSS-induced colitis in mice with enhancing intestinal barrier function and reducing colon inflammation.

Int Immunopharmacol 2021 Jul 30;99:108015. Epub 2021 Jul 30.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, China. Electronic address:

Dioscin is a natural steroid saponin derived from plants of the genus Dioscoreaceae. Previous studies have proved its effects of antibacterial, anti-inflammatory and hypolipidemic. In this study, our aim was to explore the protective effect and preliminary mechanism of Dioscin on dextran sulfate sodium (DSS)-induced colitis in mice. Read More

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Sialic acid conjugate-modified liposomes enable tumor homing of epirubicin via neutrophil/monocyte infiltration for tumor therapy.

Acta Biomater 2021 Jul 30. Epub 2021 Jul 30.

College of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China. Electronic address:

Neutrophils and monocytes (N/Ms) are potential candidates for the delivery of therapeutic agents to the tumor microenvironment (TME) because of their tumor-accumulating nature. L-selectin and Siglec-1, receptors for sialic acid (SA), are highly expressed in circulating neutrophils and monocytes, respectively, in tumor-bearing mice, and N/Ms are recruited to tumors in response to inflammatory cytokines secreted by the TME, promoting tumor growth and invasion. Therefore, we constructed a drug delivery nano-platform using N/Ms as vehicles. Read More

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PI3K/ NF-κB-dependent TNF-α and HDAC activities facilitate LPS-induced RGS10 suppression in pulmonary macrophages.

Cell Signal 2021 Jul 30:110099. Epub 2021 Jul 30.

Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA. Electronic address:

Regulator of G-protein signaling 10 (RGS10) is a member of the superfamily of RGS proteins that canonically act as GTPase activating proteins (GAPs). RGS proteins accelerate GTP hydrolysis on the G-protein α subunits and result in termination of signaling pathways downstream of G protein-coupled receptors. Beyond its GAP function, RGS10 has emerged as an anti-inflammatory protein by inhibiting LPS-mediated NF-κB activation and expression of inflammatory cytokines, in particular TNF-α. Read More

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Deficiency of Heat shock factor 4 promotes lens epithelial cell senescence through upregulating p21 expression.

Biochim Biophys Acta Mol Basis Dis 2021 Jul 30:166233. Epub 2021 Jul 30.

Jointed National Lab for antibody drug engineering, The First Affiliated Hospital, Henan University School of Medicine, Kaifeng, China; Kaifeng Key Laboratory of Myopia and cataract, Kaifeng central Hospital Kaifeng, China; The Laboratory for ophthalmology and Vision Science, Department of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. Electronic address:

Genetic mutations in heat shock factor 4 (Hsf4) is associated with both congenital and age-related cataracts. Hsf4 regulates lens development through its ability to both activate and inhibit transcription. Previous studies suggested Hsf4 is involved in modulating cellular senescence depending on p21 and p27 expression in MEF cells. Read More

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Effect of involuntary chronic physical exercise on beta-amyloid protein in experimental models of Alzheimer's disease: Systematic review and meta-analysis.

Exp Gerontol 2021 Jul 30:111502. Epub 2021 Jul 30.

Laboratório de Bioquímica e Expressão Gênica, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.

The excessive deposition of β-amyloid proteins (Aβ) is directly correlated with the establishment and development of Alzheimer's Disease (AD). Current treatments for AD only reduce symptoms instead of acting on Aβ, which is the primary etiological agent. Hence, the anti-amyloid effect of regular exercise has been widely investigated as an alternative therapy. Read More

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Lipidomic analysis of meibomian glands from type-1 diabetes mouse model and preliminary studies of potential mechanism.

Exp Eye Res 2021 Jul 30:108710. Epub 2021 Jul 30.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, 266071, China; State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China. Electronic address:

Diabetes is a significant risk factor for meibomian gland dysfunction (MGD), but its mechanism is poorly understood. The main function of the meibomian glands (MGs) is to synthesize, store, and secrete lipids. In this study, we found that the amount of lipids in the meibomian acini in STZ-induced type 1 diabetic mice decreased, and the lipid droplets became larger and irregular. Read More

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3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue.

J Vis Exp 2021 Jul 16(173). Epub 2021 Jul 16.

Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo.

Astrocytes are glial cells with an essential role in the central nervous system (CNS), including neuronal support and functionality. These cells also respond to neural injuries and act to protect the tissue from degenerative events. In vitro studies of astrocytes' functionality are important to elucidate the mechanisms involved in such events and contribute to developing therapies to treat neurological disorders. Read More

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Studying Left Ventricular Reverse Remodeling by Aortic Debanding in Rodents.

J Vis Exp 2021 Jul 14(173). Epub 2021 Jul 14.

Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto;

To better understand the left ventricular (LV) reverse remodeling (RR), we describe a rodent model wherein, after aortic banding-induced LV remodeling, mice undergo RR upon removal of the aortic constriction. In this paper, we describe a step-by-step procedure to perform a minimally invasive surgical aortic debanding in mice. Echocardiography was subsequently used to assess the degree of cardiac hypertrophy and dysfunction during LV remodeling and RR and to determine the best timing for aortic debanding. Read More

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An inducible Cldn11-CreER mouse line for selective targeting of lymphatic valves.

Genesis 2021 Aug 2:e23439. Epub 2021 Aug 2.

Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.

Luminal valves of collecting lymphatic vessels are critical for maintaining unidirectional flow of lymph and their dysfunction underlies several forms of primary lymphedema. Here, we report on the generation of a transgenic mouse expressing the tamoxifen inducible CreER under the control of Cldn11 promoter that allows, for the first time, selective and temporally controlled targeting of lymphatic valve endothelial cells. We show that within the vasculature CLDN11 is specifically expressed in lymphatic valves but is not required for their development as mice with a global loss of Cldn11 display normal valves in the mesentery. Read More

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Systemic antioxidant and anti-inflammatory effects of yellow passion fruit bagasse extract during prostate cancer progression.

J Food Biochem 2021 Aug 2:e13885. Epub 2021 Aug 2.

Faculty of Food Engineering, Department of Food and Nutrition, University of Campinas (UNICAMP), Campinas, Brazil.

We evaluated the impact of yellow passion fruit (Passiflora edulis sp.) bagasse extract (PFBE) administration in systemic oxidative and inflammatory parameters in vivo, considering prostate cancer progression in transgenic mice (TRAMP). Piceatannol, scirpusin-B, dicaffeoylquinic acid, citric acid, and (+)-catechin were identified in PFBE, and the extract showed high in vitro antioxidant capacity. Read More

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Fermitin family homolog 2 (Kindlin-2) affects vascularization during the wound healing process by regulating the Wnt/β-catenin signaling pathway in vascular endothelial cells.

Bioengineered 2021 Dec;12(1):4654-4665

Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

Kindlin-2 is a member of the FERM-containing cytoskeletal protein family that regulates cell-matrix interactions. Previous studies have shown that Kindlin-2 recruits focal adhesion proteins and regulates integration by binding to the focal adhesion region of the integrin β-segment. Although Kindlin-2 has been reported to be involved in various skin diseases and many kinds of tumors, its role in the skin wound healing process remains unclear. Read More

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December 2021

New benzothieno[2,3-]pyridines as non-steroidal CYP17 inhibitors: design, synthesis, anticancer screening, apoptosis induction, and ADME profile studies.

J Enzyme Inhib Med Chem 2021 Dec;36(1):1839-1859

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Menoufia University, Menoufia, Egypt.

A series of [1]benzothieno[2,3-]pyridines was synthesised. Most compounds were chosen by NCI-USA to evaluate their anticancer activity. Compounds showed prominent growth inhibition against most cell lines. Read More

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December 2021

A quick and low-intensity method for oral administration to large numbers of mice: A possible alternative to oral gavage.

Lab Anim 2021 Aug 1:236772211035250. Epub 2021 Aug 1.

Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Portugal.

Oral administration of medication to experimental animals is a cause of significant stress. When coupled to animals who are already under strenuous circumstances due to the disease being modelled, there is a significant risk for increased morbidity and mortality, thus influencing the results. Faced with these constraints, a low-intensity method for oral administration was developed, based solely on the natural behaviour of the animals and minimal conditioning, in which precise doses of medication were administered in a locally available, standard wheat cookie fragment, providing both a palatable vehicle and an absorbent matrix for the medication. Read More

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Refining the rat sciatic nerve block: a novel ultrasound-guided technique.

Lab Anim 2021 Aug 1:236772211034627. Epub 2021 Aug 1.

Department of Anesthesiology and Pain Medicine, 19951Naval Medical Center Portsmouth, Naval Medical Center Portsmouth, USA.

Murine translational models are an important tool to understand pain pathophysiology. One procedure used frequently in murine research is the sciatic nerve block. This study sought to demonstrate the use of ultrasound-guided sciatic nerve block in a cadaveric murine model. Read More

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Aging-related hyperphosphatemia impairs myogenic differentiation and enhances fibrosis in skeletal muscle.

J Cachexia Sarcopenia Muscle 2021 Aug 1. Epub 2021 Aug 1.

Departamento de Biología de Sistemas, Facultad de Medicina y Ciencias de la Salud, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.

Background: Hyperphosphatemia has been related to the development of sarcopenia in aging mice. We describe the intracellular mechanisms involved in the impairment of the myogenic differentiation promoted by hyperphosphatemia and analyse these mechanisms in the muscle from older mice.

Methods: C C cells were grown in 2% horse serum in order to promote myogenic differentiation, in the presence or absence of 10 mM beta-glycerophosphate (BGP) for 7 days. Read More

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A NEW approach for characterizing mouse urinary pathophysiologies.

Physiol Rep 2021 Aug;9(15):e14964

Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, USA.

The void spot assay (VSA) is a cost-effective method for evaluating and quantifying mouse urinary voiding phenotypes. The VSA has been used to differentiate voiding behaviors between experimental groups, but not as a diagnostic assay. To build toward this goal, we used the VSA to define voiding patterns of male mice with diabetic diuresis (BTBR. Read More

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Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake.

STAR Protoc 2021 Sep 17;2(3):100496. Epub 2021 Jul 17.

State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.

The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a protocol accessing AMPK activity in mice by measuring glucose uptake induced by AMPK activators, MK8722 and A769662. Read More

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September 2021

Neprilysin Inhibition Increases Glucagon Levels in Humans and Mice With Potential Effects on Amino Acid Metabolism.

J Endocr Soc 2021 Sep 16;5(9):bvab084. Epub 2021 May 16.

Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen,Denmark.

Context: Inhibitors of the protease neprilysin (NEP) are used for treating heart failure, but are also linked to improvements in metabolism. NEP may cleave proglucagon-derived peptides, including the glucose and amino acid (AA)-regulating hormone glucagon. Studies investigating NEP inhibition on glucagon metabolism are warranted. Read More

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September 2021

Bifidobacterium Longum: Protection against Inflammatory Bowel Disease.

J Immunol Res 2021 23;2021:8030297. Epub 2021 Jul 23.

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.

The prevalence of inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD), increases gradually worldwide in the past decades. IBD is generally associated with the change of the immune system and gut microbiota, and the conventional treatments usually result in some side effects. Bifidobacterium longum, as colonizing bacteria in the intestine, has been demonstrated to be capable of relieving colitis in mice and can be employed as an alternative or auxiliary way for treating IBD. Read More

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Physiological Functions of Mcl-1: Insights From Genetic Mouse Models.

Front Cell Dev Biol 2021 16;9:704547. Epub 2021 Jul 16.

Programme in Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore, Singapore.

The ability to regulate the survival and death of a cell is paramount throughout the lifespan of a multicellular organism. Apoptosis, a main physiological form of programmed cell death, is regulated by the Bcl-2 family proteins that are either pro-apoptotic or pro-survival. The functions of distinct Bcl-2 family members are largely unmasked by genetically engineered murine models. Read More

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Knockout Gene-Based Evidence for PIWI-Interacting RNA Pathway in Mammals.

Front Cell Dev Biol 2021 14;9:681188. Epub 2021 Jul 14.

State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China.

The PIWI-interacting RNA (piRNA) pathway mainly consists of evolutionarily conserved protein factors. Intriguingly, many mutations of piRNA pathway factors lead to meiotic arrest during spermatogenesis. The majority of piRNA factor-knockout animals show arrested meiosis in spermatogenesis, and only a few show post-meiosis male germ cell arrest. Read More

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The Lipopolysaccharide-Sensing Caspase(s)-4/11 Are Activated in Cirrhosis and Are Causally Associated With Progression to Multi-Organ Injury.

Front Cell Dev Biol 2021 15;9:668459. Epub 2021 Jul 15.

Institute for Liver and Digestive Health, UCL, London, United Kingdom.

Background And Aims: The development of multi-organ injury in cirrhosis is associated with increased intestinal permeability, translocation of gut-derived bacterial products [e.g., lipopolysaccharide (LPS)] into the circulation, and increased non-apoptotic hepatocyte cell death. Read More

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Reversing Epigenetic Gene Silencing to Overcome Immune Evasion in CNS Malignancies.

Front Oncol 2021 15;11:719091. Epub 2021 Jul 15.

Neuro-Oncology Branch, National Cancer Institute, Bethesda, MD, United States.

Glioblastoma (GBM) is an aggressive brain malignancy with a dismal prognosis. With emerging evidence to disprove brain-immune privilege, there has been much interest in examining immunotherapy strategies to treat central nervous system (CNS) cancers. Unfortunately, the limited success of clinical studies investigating immunotherapy regimens, has led to questions about the suitability of immunotherapy for these cancers. Read More

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Down-Regulation of Toll-Like Receptor 5 (TLR5) Increased VEGFR Expression in Triple Negative Breast Cancer (TNBC) Based on Radionuclide Imaging.

Front Oncol 2021 15;11:708047. Epub 2021 Jul 15.

Key Laboratory for Experimental Teratology of the Ministry of Education and Research Center for Experimental Nuclear Medicine, School of Basic Medical Sciences, Shandong University, Jinan, China.

In this study, GFP-tagged TNBC 4T1 cells with down-regulated TLR5 expression (TLR5 4T1) and normal TLR5 expression (TLR5 4T1) were constructed, respectively. RT-PCR and Western blot studies showed that down-regulation of TLR5 obviously increased the expression of VEGFR in 4T1 cells. Highly stable radio-probes I-anti-TLR5 mAb/I-VEGF/I-IgG were obtained with labeling rates over 85% and radiochemical purities above 90%. Read More

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Targeting Thymidylate Synthase Enhances the Chemosensitivity of Triple-Negative Breast Cancer Towards 5-FU-Based Combinatorial Therapy.

Front Oncol 2021 15;11:656804. Epub 2021 Jul 15.

Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India.

Background: The ongoing treatment modalities for breast cancer (BC) primarily rely on the expression status of ER, PR and HER-2 receptors in BC tissues. Our strategy of chemosensitization provides new insights to counter chemoresistance, a major obstacle that limits the benefits of chemotherapy of mammary cancers.

Methods: By utilizing a murine breast cancer model employing NSG mice bearing orthotopic triple-negative breast cancer (TNBC) xenografts, we have evaluated the ability of phytochemical curcumin in chemosensitizing BC to 5-Fluorouracil (5-FU) chemotherapy and the differential modulations of cellular events in response to this strategy, independent of their receptor status. Read More

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Adiponectin Treatment Attenuates Cerebral Ischemia-Reperfusion Injury through HIF-1-Mediated Antioxidation in Mice.

Oxid Med Cell Longev 2021 14;2021:5531048. Epub 2021 Jul 14.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Adiponectin (ADPN) plays an important role in cerebral ischemia-reperfusion injury. Although previous studies have confirmed that ADPN pretreatment has a protective effect on ischemic stroke, the therapeutic effect of ADPN on ischemic stroke and the underlying mechanism are still unclear. In order to clarify these questions, focal transient cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice and ADPN was administered for three times at 6 h, 24 h, and 48 h after reperfusion. Read More

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Exosomal lncRNA-H19 promotes osteogenesis and angiogenesis through mediating Angpt1/Tie2-NO signaling in CBS-heterozygous mice.

Theranostics 2021 22;11(16):7715-7734. Epub 2021 Jun 22.

Bone Biology Laboratory, Department of Physiology, School of Medicine, University of Louisville, Louisville, KY 40202, USA.

Emerging evidence indicates that the growth of blood vessels and osteogenesis is tightly coordinated during bone development. However, the molecular regulators of intercellular communication in the bone microenvironment are not well studied. Therefore, we aim to investigate whether BMMSC-Exo promotes osteogenesis and angiogenesis via transporting lnc-H19 in the CBS- heterozygous mouse model. Read More

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Effects of intermittent fasting on liver physiology and metabolism in mice.

Exp Ther Med 2021 Sep 5;22(3):950. Epub 2021 Jul 5.

Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, Gansu 730030, P.R. China.

A broad spectrum of health benefits from intermittent fasting have been reported in studies on animal models and human subjects. However, the underlying mechanisms of these beneficial effects remain largely elusive. The present study aimed to explore the effects and potential mode of action of intermittent fasting in mouse models with a focus on the liver. Read More

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September 2021

MicroRNA-146a overexpression alleviates intestinal ischemia/reperfusion-induced acute lung injury in mice.

Exp Ther Med 2021 Sep 1;22(3):937. Epub 2021 Jul 1.

Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong 518028, P.R. China.

Previous studies have shown that microRNAs (miRs), such as miR-146a play an important role in the pathogenesis of intestinal ischemia/reperfusion (I/R)-induced injury; however, the role of miR-146a in intestinal I/R-induced acute lung injury has not been elucidated. An intestinal I/R-induced injury mouse model was established in the present study by clamping the superior mesenteric artery and expression levels of miR-146a in intestinal and lung tissue samples were evaluated using reverse transcription-quantitative PCR (RT-qPCR). Intestinal and lung histopathological characteristics in mice with intestinal I/R-induced injury were assessed by hematoxylin and eosin staining, and mRNA and protein expression levels in intestinal and lung tissue samples were evaluated using RT-qPCR and western blotting, respectively. Read More

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September 2021