63,395 results match your criteria structures identified


Localized scleroderma: actual insights and new biomarkers.

Int J Dermatol 2021 Aug 4. Epub 2021 Aug 4.

I.M. Sechenov First Moscow State Medical University (Sechenov University), Department of Dermatovenerology, Moscow, Russia.

Localized scleroderma (LS, morphea, limited scleroderma, focal scleroderma) is a chronic autoimmune disease characterized by a progressive damage to the connective tissue with a predominance of fibrosclerotic disorders in the skin and the subcutaneous tissue. In addition surrounding structures may be affected: fascia, muscle, and bone tissues. This review reflects the current understanding about limited scleroderma, its pathogenesis, diagnosis, new biomarkers, and information about the possibilities of its transition to systemic scleroderma. Read More

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Deep geometric representations for modeling effects of mutations on protein-protein binding affinity.

PLoS Comput Biol 2021 Aug 4;17(8):e1009284. Epub 2021 Aug 4.

Department of Computer Science, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.

Modeling the impact of amino acid mutations on protein-protein interaction plays a crucial role in protein engineering and drug design. In this study, we develop GeoPPI, a novel structure-based deep-learning framework to predict the change of binding affinity upon mutations. Based on the three-dimensional structure of a protein, GeoPPI first learns a geometric representation that encodes topology features of the protein structure via a self-supervised learning scheme. Read More

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Diffuse midline gliomas, H3 K27M-mutant are associated with less peritumoral edema and contrast enhancement in comparison to glioblastomas, H3 K27M-wildtype of midline structures.

PLoS One 2021 4;16(8):e0249647. Epub 2021 Aug 4.

Radiology and Neuroradiology, Asklepios Klinik Barmbek, Hamburg, Germany.

Purpose: The entity 'diffuse midline glioma, H3 K27M-mutant (DMG)' was introduced in the revised 4th edition of the 2016 WHO classification of brain tumors. However, there are only a few reports on magnetic resonance imaging (MRI) of these tumors. Thus, we conducted a retrospective survey focused on MRI features of DMG compared to midline glioblastomas H3 K27M-wildtype (mGBM-H3wt). Read More

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Psychometric Properties of the Safety Climate Survey in Austrian Acute Care: Factor Structure, Reliability, and Usability.

J Patient Saf 2021 Aug 2. Epub 2021 Aug 2.

From the Paracelsus Medical University, Institute of Nursing Science and Practice, Salzburg, Austria.

Background: Hospitals are complex organizations with a potential for medical errors that can be influenced by safety culture. Safety climate, as measurable element of safety culture, illustrates the perception of safety-relevant aspects of health care staff at a certain time. The Safety Climate Survey is applied internationally to measure safety climate. Read More

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The Arcuate Eminence and Superior Semicircular Canal: Magnetic Resonance Imaging Study.

J Comput Assist Tomogr 2021 Aug 2. Epub 2021 Aug 2.

From the Department of Neurological Surgery, Juntendo University Urayasu Hospital, Urayasu, Chiba Division of Radiological Technology, Medical Satellite Yaesu Clinic, Tokyo, Japan.

Objective: The present study aimed to explore the relationship between the arcuate eminence (AE) and superior semicircular canal (SSC) using the constructive interference steady-state (CISS) sequence.

Patients And Methods: After conventional magnetic resonance imaging, a total of 71 patients underwent the CISS sequence in thin-sliced coronal sections.

Results: In all patients, the SSC was delineated on both sides. Read More

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Enzymatic Synthesis of l--β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Y0017.

Appl Environ Microbiol 2021 Aug 4:AEM0133521. Epub 2021 Aug 4.

Research Institute for Science and Engineering, Waseda University, Tokyo, Japan.

β-Hydroxy-α-amino acids are useful compounds for pharmaceutical development. Enzymatic synthesis of β-hydroxy-α-amino acids has attracted considerable interest as a selective, sustainable, and environmentally benign process. In this study, we identified a novel amino acid hydroxylase, AEP14369, from Y0017, which is included in a previously constructed CAS-like superfamily protein library, to widen the variety of amino acid hydroxylases. Read More

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Sequestration of the exocytic SNARE Psy1 into multiprotein nodes reinforces polarized morphogenesis in fission yeast.

Mol Biol Cell 2021 Aug 4:mbcE20050277. Epub 2021 Aug 4.

Department of Biochemistry and Cell Biology, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755.

Polarized morphogenesis is achieved by targeting or inhibiting growth at distinct regions. Rod-shaped fission yeast cells grow exclusively at their ends by restricting exocytosis and secretion to these sites. This growth pattern implies the existence of mechanisms that prevent exocytosis and growth along non-growing cell sides. Read More

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Guest-Host Interactions in Clathrate Hydrates: Benchmark MP2 and CCSD(T)/CBS Binding Energies of CH, CO, and HS in (HO) Cages.

J Phys Chem Lett 2021 Aug 4:7574-7582. Epub 2021 Aug 4.

Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.

We present benchmark binding energies of naturally occurring gas molecules CH, CO, and HS in the small cage, namely, the pentagonal dodecahedron (5) (HO), which is one of the constituent cages of the 3 major lattices (structures I, II, and H) of clathrate hydrates. These weak interactions require higher levels of electron correlation and converge slowly with an increasing basis set to the complete basis set (CBS) limit, necessitating the use of large basis sets up to the aug-cc-pV5Z and subsequent correction for basis set superposition error (BSSE). For the host hollow (HO) cages, we have identified a most stable isomer with binding energy of -200. Read More

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Chemical Characterization of Recycled Consumer Products Using Suspect Screening Analysis.

Environ Sci Technol 2021 Aug 4. Epub 2021 Aug 4.

Center for Computational Toxicology and Exposure, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27709, United States.

Recycled materials are found in many consumer products as part of a circular economy; however, the chemical content of recycled products is generally uncharacterized. A suspect screening analysis using two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS) was applied to 210 products (154 recycled, 56 virgin) across seven categories. Chemicals in products were tentatively identified using a standard spectral library or confirmed using chemical standards. Read More

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TMEM106B in humans and Vac7 and Tag1 in yeast are predicted to be lipid transfer proteins.

Authors:
Tim P Levine

Proteins 2021 Aug 4. Epub 2021 Aug 4.

UCL Institute of Ophthalmology, London, United Kingdom.

TMEM106B is an integral membrane protein of late endosomes and lysosomes involved in neuronal function, its over-expression being associated with familial frontotemporal lobar degeneration, and point mutation linked to hypomyelination. It has also been identified in multiple screens for host proteins required for productive SARS-CoV2 infection. Because standard approaches to understand TMEM106B at the sequence level find no homology to other proteins, it has remained a protein of unknown function. Read More

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Identification of a proline-kinked amphipathic α-helix downstream from the methyltransferase domain of a potexvirus replicase and its role in virus replication and perinuclear complex formation.

J Virol 2021 Aug 4:JVI0190620. Epub 2021 Aug 4.

Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Japan.

Characterized positive-strand RNA viruses replicate in association with intracellular membranes. Regarding viruses in the genus , the mechanism by which their RNA-dependent RNA polymerase (replicase) associates with membranes is understudied. Here, by membrane flotation analyses of the replicase of plantago asiatica mosaic potexvirus (PlAMV), we identified a region in the methyltransferase (MET) domain as a membrane association determinant. Read More

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Double-Walled Metal-Organic Framework with Regulable Pore Environments for Efficient Removal of Radioactive Cesium Cations.

Inorg Chem 2021 Aug 4. Epub 2021 Aug 4.

School of Materials Science and Engineering, Tianjin Key Laboratory of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin 300350, China.

An anion double-walled metal-organic framework [CoLi(BTC)(DMF)(HO)·(CH)N] () based on heterobimetallic Li and Co ions was successfully constructed. Utilizing selective destruction and formation of Co-O/Co-N bonds in the metal chains, [CoLi(BTC)(py)(HO)·(CH)N] () and [CoLi(BTC)(pi)(HO)·(CH)N] () with the same skeleton but distinct pore structures can be surprisingly obtained. Additionally, compounds and can be transformed into [CoLi(BTC)(HO)·(CH)N] () by soaking them in an ethanol solution. Read More

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Interaction mechanism of endogenous PP2A inhibitor protein ENSA with PP2A.

FEBS J 2021 Aug 4. Epub 2021 Aug 4.

Institute of Biomedicine, University of Turku, 20520, Turku, Finland.

The vast diversity of protein phosphatase 2A (PP2A) holoenzyme composition ensures its multi-faceted role in the regulation of cellular growth and signal transduction. In several pathological conditions, such as cancer, PP2A is inhibited by endogenous inhibitor proteins. Several PP2A inhibitor proteins have been identified, one of which is α-endosulfine (ENSA). Read More

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Using yeast two-hybrid system and molecular dynamics simulation to detect venom protein-protein interactions.

Curr Res Toxicol 2021 23;2:93-98. Epub 2021 Feb 23.

Biology Department, The University of Texas Rio Grande Valley, Brownsville, TX 78520, USA.

Proteins and peptides are major components of snake venom. Venom protein transcriptomes and proteomes of many snake species have been reported; however, snake venom complexity (i.e. Read More

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February 2021

DESCARTES and his project of a fantasized brain.

Dement Neuropsychol 2021 Apr-Jun;15(2):281-285

Institute of Neurology Deolindo Couto, Universidade Federal do Rio de Janeiro - Rio de Janeiro, RJ, Brazil.

Interest in anatomy dates from the earliest times. Such knowledge was acquired through dissections of animals and human corpses by many researchers. The macroscopic anatomy of the varied structures of the brain were identified over the centuries, and the predominating solid substance was seen as amorphous, and devoid of any specific function, until the Renaissance. Read More

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January 2021

Seizure Outcome after surgery for Insula High Grade Glioma.

World Neurosurg 2021 Jul 31. Epub 2021 Jul 31.

Dept of Neurosurgery, University Hospital of North Midlands, Stoke on Trent, UK.

Background: The insula cortex is an eloquent island of mesocortex surrounded by vital structures making this region relatively challenging to the neurosurgeon. Historically, lesions in this region were considered too high risk to approach given the strong chance of poor surgical outcome. Advances over the last few decades have meant that surgeons can more safely access this eloquent region. Read More

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A reflux-and-growth mechanism explains oscillatory patterning of lateral root branching sites.

Dev Cell 2021 Jul 27. Epub 2021 Jul 27.

Computational Developmental Biology, Department of Biology, Utrecht University, Utrecht, the Netherlands. Electronic address:

Modular, repetitive structures are a key component of complex multicellular body plans across the tree of life. Typically, these structures are prepatterned by temporal oscillations in gene expression or signaling. Although a clock-and-wavefront mechanism was identified and plant leaf phyllotaxis arises from a Turing-type patterning for vertebrate somitogenesis and arthropod segmentation, the mechanism underlying lateral root patterning has remained elusive. Read More

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New sesquiterpene dilactone and -carboline alkaloid and the -glucosidase inhibitory activity of selected phytochemicals from (L.) Kosterm.

Nat Prod Res 2021 Aug 3:1-9. Epub 2021 Aug 3.

Department of Chemistry, Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia.

One new sesquiterpene dilactone, coccinine () and one new -carboline alkaloid, daibucarboline F () together with 10 known compounds; linderane (), linderalactone (), pseudoneolinderane (), linderanlide C (), linderanine A (), epicatechin (), (-)-taxifolin (), astilbin (), L-quercitrin () and afzelin () were isolated from the stems and leaves of (L.) Kosterm (Lauraceae). The structures of ( and ) were established by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in literature. Read More

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Hot Carrier Dynamics at Ligated Silicon(111) Surfaces: A Computational Study.

J Phys Chem Lett 2021 Aug 3:7504-7511. Epub 2021 Aug 3.

Department of Chemistry and Biochemistry, North Dakota State University, Fargo, North Dakota 58108, United States.

We provide a case-study for thermal grafting of benzenediazonium bromide onto a hydrogenated Si(111) surface using molecular dynamics (AIMD) calculations. A sequence of reaction steps is identified in the AIMD trajectory, including the loss of N from the diazonium salt, proton transfer from the surface to the bromide ion that eliminates HBr, and deposition of the phenyl group onto the surface. We next assess the influence of the phenyl groups on photophysics of hydrogen-terminated Si(111) slabs. Read More

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Novel factor VII gene mutations in six families with hereditary coagulation factor VII deficiency.

J Clin Lab Anal 2021 Aug 2:e23905. Epub 2021 Aug 2.

Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Introduction: Hereditary human coagulation factor VII (FVII) deficiency is an inherited autosomal recessive hemorrhagic disease involving mutations in the F7 gene. The sites and types of F7 mutations may influence the coagulation activities of plasma FVII (FVII: C) and severity of hemorrhage symptoms. However, the specific mutations that impact FVII activity are not completely known. Read More

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An Unusual Maxillary Tumor with Tubuloductal Epithelial Structures, Solid Epithelial Nests and Stromal Odontogenic Ameloblast-Associated Protein Deposits. Tubuloductal/Syringoid Variant of Central Odontogenic Fibroma with Amyloid?

Head Neck Pathol 2021 Aug 2. Epub 2021 Aug 2.

Faculty of Dental Medicine, Université de Montréal, Montréal, Québec, H3T 1J4, Canada.

Glandular tumors of jaw bones present, most often, histopathologic features of salivary gland and, rarely, of cutaneous glandular neoplasms. They are thought to originate from odontogenic epithelium. An unusual maxillary tumor presenting as a radiolucency in the periapical area of the right permanent lateral incisor of a 74-year-old male is presented causing root resorption. Read More

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The "Visible" Muscles on Ultrasound Imaging Make Botulinum Toxin Injection More Precise: A Systematic Review.

Aesthetic Plast Surg 2021 Aug 2. Epub 2021 Aug 2.

Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Dongcheng District, Beijing, 100730, China.

Background: Botulinum toxin (BoNT) injection is the most commonly performed procedure in cosmetic surgery. However, blind injection is unable to take individual anatomical variations into consideration, which is the main contributing factor to complications. Ultrasound (US) imaging was introduced to reduce complications and improve effects. Read More

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Distinct mutations in importin-β family nucleocytoplasmic transport receptors transportin-SR and importin-13 affect specific cargo binding.

Sci Rep 2021 Aug 2;11(1):15649. Epub 2021 Aug 2.

Cellular Dynamics Laboratory, RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan.

Importin-(Imp)β family nucleocytoplasmic transport receptors (NTRs) are supposed to bind to their cargoes through interaction between a confined interface on an NTR and a nuclear localization or export signal (NLS/NES) on a cargo. Although consensus NLS/NES sequence motifs have been defined for cargoes of some NTRs, many experimentally identified cargoes of those NTRs lack those motifs, and consensus NLSs/NESs have been reported for only a few NTRs. Crystal structures of NTR-cargo complexes have exemplified 3D structure-dependent binding of cargoes lacking a consensus NLS/NES to different sites on an NTR. Read More

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Structures of the Plasmodium falciparum heat-shock protein 70-x ATPase domain in complex with chemical fragments identify conserved and unique binding sites.

Acta Crystallogr F Struct Biol Commun 2021 Aug 28;77(Pt 8):262-268. Epub 2021 Jul 28.

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.

Plasmodium falciparum invades erythrocytes and extensively modifies them in a manner that increases the virulence of this malaria parasite. A single heat-shock 70 kDa-type chaperone, PfHsp70-x, is among the parasite proteins exported to the host cell. PfHsp70-x assists in the formation of a key protein complex that underpins parasite virulence and supports parasite growth during febrile episodes. Read More

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Proteomics of pseudoexfoliation materials in the anterior eye segment.

Adv Protein Chem Struct Biol 2021 26;127:271-290. Epub 2021 Apr 26.

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, United States; Vision Science and Investigative Ophthalmology Graduate Program, University of Miami, Miami, FL, United States; Miami Integrative Metabolomics Research Center, University of Miami, Miami, FL, United States. Electronic address:

Pseudoexfoliation syndrome (PEX) is characterized by the production of white extracellular fluffy clumps of microfibrillar material that aggregates in various organs throughout the body but is known to cause disease in the eye. The accumulation of PEX material (PEXM) in the anterior segment ocular structures is believed to cause an increase in intraocular pressure (IOP) resulting in pseudoexfoliation glaucoma (PEXG). The onset of PEXG is often bilateral but asymmetric-one eye often presents with glaucoma prior to the other eye. Read More

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Clinical governance in New Zealand: perceptions from registered health professionals in health care delivery compared with social insurance.

Aust Health Rev 2021 Aug 3. Epub 2021 Aug 3.

ObjectiveEffective clinical governance can improve delivery of health outcomes. This exploratory study compared perceptions of clinical governance development held by registered health professionals employed by two different but interrelated health organisations in the broader New Zealand (NZ) health system. Most staff in public sector healthcare service delivery organisations (i. Read More

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Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius.

mBio 2021 Aug 3:e0136821. Epub 2021 Aug 3.

Biosciences Institute, The Medical School, Newcastle University, Newcastle upon Tyne, United Kingdom.

The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins (AGPs) are a ubiquitous feature of plant polysaccharides available to the HGM. Read More

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Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains.

mBio 2021 Aug 3:e0159021. Epub 2021 Aug 3.

Department of Microbiology and Immunology, Loyola University Chicagogrid.164971.c, Maywood, Illinois, USA.

Selective pressures drive adaptive changes in the coronavirus spike proteins directing virus-cell entry. These changes are concentrated in the amino-terminal domains (NTDs) and the receptor-binding domains (RBDs) of complex modular spike protein trimers. The impact of this hypervariability on virus entry is often unclear, particularly with respect to sarbecovirus NTD variations. Read More

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Advanced imaging of dentin microstructure.

Biomed Phys Eng Express 2021 Aug 2. Epub 2021 Aug 2.

Restorative Dentistry, King Abdulaziz University, Alnaeem, Jeddah, 21418, SAUDI ARABIA.

This study aimed to demonstrate the feasibility of using optical coherence tomography (OCT) for locating the sectioning site of a specimen before characterizing the ultrastructural features of dentin surfaces as well as the inner wall of the dentinal tubules (DT) using a field emission scanning electron microscope (FESEM). Eight sound human molar teeth were extracted, examined via cross-polarization optical coherence tomography (CP-OCT), embedded, and hemisectioned using a low-speed diamond sawing machine. Next, each sectioned surface was further trimmed, polished, and examined under a confocal laser scanning microscope (CLSM) to locate the target area on the superficial dentin. Read More

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Characterization and expression analysis of wall-associated kinase (WAK) and WAK-like family in cotton.

Int J Biol Macromol 2021 Jul 30. Epub 2021 Jul 30.

State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton of the Ministry of Agriculture and Rural Affairs, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

The wall-associated kinases (WAKs) and WAK-like kinases (WAKLs) form a group of receptor-like kinases (RLKs) with extracellular domains tightly linked to the cell wall. The WAKs/WAKLs have been known to be involved in plant growth, development, and stress responses. However, the functions of WAKs/WAKLs are less well known in cotton. Read More

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