128,284 results match your criteria stress conditions

Investigation and Analysis of Perceived Stress Levels in Pediatric Nurses During the 2019-20 SARS-CoV-2 Outbreak.

Holist Nurs Pract 2021 May-Jun 01;35(3):133-139

Department of Infectious Diseases, Children's Hospital of Soochow University, Suzhou, China.

This study investigated perceived stress levels of pediatric nurses during the 2019-20 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak. From February 9 to 13, 2020, 250 pediatric nurses were selected from 4 grade III children's hospitals and 5 grade III general hospitals in Jiangsu Province, China. A general information questionnaire and a stress perception scale were used to investigate and analyze the influencing factors of perceived stress among participants. Read More

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The 7SK/P-TEFb snRNP controls ultraviolet radiation-induced transcriptional reprogramming.

Cell Rep 2021 Apr;35(2):108965

Molecular, Cellular and Developmental Biology Department (MCD), Centre de Biologie Intégrative (CBI), University of Toulouse, CNRS, UPS, 31062 Toulouse, France. Electronic address:

Conversion of promoter-proximally paused RNA polymerase II (RNAPII) into elongating polymerase by the positive transcription elongation factor b (P-TEFb) is a central regulatory step of mRNA synthesis. The activity of P-TEFb is controlled mainly by the 7SK small nuclear ribonucleoprotein (snRNP), which sequesters active P-TEFb into inactive 7SK/P-TEFb snRNP. Here we demonstrate that under normal culture conditions, the lack of 7SK snRNP has only minor impacts on global RNAPII transcription without detectable consequences on cell proliferation. Read More

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Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.

Cell Rep 2021 Apr;35(2):108977

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Sevilla 41092, Spain; Topology and DNA Breaks Group, Spanish National Cancer Centre (CNIO), Madrid 28029, Spain. Electronic address:

Accumulation of topological stress in the form of DNA supercoiling is inherent to the advance of RNA polymerase II (Pol II) and needs to be resolved by DNA topoisomerases to sustain productive transcriptional elongation. Topoisomerases are therefore considered positive facilitators of transcription. Here, we show that, in contrast to this general assumption, human topoisomerase IIα (TOP2A) activity at promoters represses transcription of immediate early genes such as c-FOS, maintaining them under basal repressed conditions. Read More

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Systematic Design, Synthesis, and Biological Studies of Some Novel 1,4-Benzoquinone Derivatives for the Prospective Management of Cognitive Decline.

ACS Chem Neurosci 2021 Apr 14. Epub 2021 Apr 14.

Department of Pharmaceutical Chemistry, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.

Cholinesterases are significant biological targets for the regulation of cholinergic neurotransmission, and their inhibitors are being exploited for the management of cognitive decline in various neurological conditions. The 1,4-benzoquinone scaffold possesses antioxidant potential along with AChE inhibition activity in various neurological disorders. To design novel and potent selective 1,4-benzoquinone analogues as cholinesterase inhibitors, a ligand-based drug design strategy was followed to develop a 3D quantitative structure-selectivity relationship (QSSR) model. Read More

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Effects of CcpA against salt stress in Lactiplantibacillus plantarum as assessed by comparative transcriptional analysis.

Appl Microbiol Biotechnol 2021 Apr 14. Epub 2021 Apr 14.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, People's Republic of China.

Lactiplantibacillus plantarum is frequently exposed to salt stress during industrial applications. Catabolite control protein (CcpA) controls the transcription of many genes, but its role in the response to salt stress remains unclear. In this study, we used transcriptome analyses to investigate differences in the logarithmic growth phases of Lactiplantibacillus plantarum ST-III and its ccpA-knockout mutant when grown with or without salt and glycine betaine (GB). Read More

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Rice drought breeding has selected for longer flag leaves and lower stomatal density.

J Exp Bot 2021 Apr 14. Epub 2021 Apr 14.

International Rice Research Institute, Los Baños, Laguna, Philippines.

Direct selection for yield under drought has resulted in the release of a number of drought-tolerant rice varieties across Asia. In this study, we characterized physiological traits affected by that strategy in breeding trials across sites in Bangladesh, India, and Nepal. The drought breeding lines and drought-tolerant varieties showed consistently longer flag leaves and lower stomatal density than the drought-susceptible check, IR64. Read More

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RhoG-Rac1 Signaling Pathway Mediates Metabolic Dysfunction of the Pancreatic Beta-Cells Under Chronic Hyperglycemic Conditions.

Cell Physiol Biochem 2021 Apr;55(2):180-192

Biomedical Research Service, John D. Dingell VA Medical Center, Detroit, MI, USA,

Background/aims: Published evidence suggests regulatory roles for small G proteins (Cdc42 and Rac1) in glucose-stimulated insulin secretion (GSIS) from pancreatic beta-cells. More recent evidence suggests novel roles for these G proteins, specifically Rac1, in the induction of metabolic dysfunction of the islet beta-cell under the duress of a variety of stress conditions. However, potential upstream regulators of sustained activation of Rac1 have not been identified in the beta-cell. Read More

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Pediatric sarcoma survivorship: A call for a developmental cascades approach.

Dev Psychopathol 2021 Apr 14:1-10. Epub 2021 Apr 14.

Department of Psychology, Gonzaga University, Spokane, WA, USA.

Survivors of pediatric sarcomas often experience greater psychological and psychosocial difficulties than their non-afflicted peers. We consider findings related to poorer outcomes from a developmental cascade perspective. Specifically, we discuss how physical, neurocognitive, psychological, and psychosocial costs associated with pediatric sarcomas and their treatment function transactionally to degrade well-being in long-term pediatric sarcoma survivors. Read More

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The Survey of the Health of Wisconsin (SHOW) Program: An infrastructure for Advancing Population Health Sciences.

medRxiv 2021 Apr 7. Epub 2021 Apr 7.

Purpose: The Survey of the Health of Wisconsin (SHOW) was established in 2008 by the University of Wisconsin (UW) School of Medicine and Public Health (SMPH) with the goals of 1) providing a timely and accurate picture of the health of the state residents; and 2) serving as an agile resource infrastructure for ancillary studies. Today SHOW continues to serve as a vital population health research infrastructure.

Participants: SHOW currently includes 5,846 adult and 980 minor participants recruited between 2008-2019 in four primary waves. Read More

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The Anaesthetic Biobank of Cerebrospinal fluid: a unique repository for neuroscientific biomarker research.

Ann Transl Med 2021 Mar;9(6):455

Department of Anesthesiology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Background: The pathophysiology of numerous central nervous system disorders remains poorly understood. Biomarker research using cerebrospinal fluid (CSF) is a promising way to illuminate the neurobiology of neuropsychiatric disorders. CSF biomarker studies performed so far generally included patients with neurodegenerative diseases without an adequate control group. Read More

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Transcriptome-wide and expression analysis of the NAC gene family in pepino () during drought stress.

PeerJ 2021 29;9:e10966. Epub 2021 Mar 29.

Qinghai Key Laboratory of Vegetable Genetics and Physiology, Agriculture and Forestry Sciences Institute of Qinghai University, Qinghai University, Xining, P.R. China.

(Pepino) is an increasingly popular solanaceous crop and is tolerant of drought conditions. In this study, 71 NAC transcription factor family genes of were selected to provide a theoretical basis for subsequent in-depth study of their regulatory roles in the response to biological and abiotic stresses, and were subjected to whole-genome analysis. The NAC sequences obtained by transcriptome sequencing were subjected to bioinformatics prediction and analysis. Read More

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Atrial fibrillation and autonomic nervous system: A translational approach to guide therapeutic goals.

J Arrhythm 2021 Apr 9;37(2):320-330. Epub 2021 Feb 9.

Department of Cardiology Policlinico Casilino Rome Italy.

The autonomic nervous system (ANS) is known to play an important role in the genesis and maintenance of atrial fibrillation (AF). Biomolecular and genetic mechanisms, anatomical knowledges with recent diagnostic techniques acquisitions, both invasive and non-invasive, have enabled greater therapeutic goals in patients affected by AF related to ANS imbalance. Catheter ablation of ganglionated plexi (GP) in the left and right atrium has been proposed in varied clinical conditions. Read More

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Metformin attenuates diabetic renal injury via the AMPK-autophagy axis.

Exp Ther Med 2021 Jun 31;21(6):578. Epub 2021 Mar 31.

Department of Nephrology, General Hospital of Daqing Oil Field, Daqing, Heilongjiang 163001, P.R. China.

Diabetic nephropathy (DN) is a clinical condition characterized by kidney damage that is observed in patients with diabetes. DN is the main cause of end-stage renal disease (ESRD), which is the final stage of chronic kidney disease. Increasing evidence suggests that metformin, a characteristic oral hypoglycemic drug used for treating diabetes, exerts beneficial effects on various medical conditions and diseases, including cancer, cardiovascular diseases and thyroid-related disorders. Read More

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Low biological fluctuation of mitochondrial CpG and non-CpG methylation at the single-molecule level.

Sci Rep 2021 Apr 13;11(1):8032. Epub 2021 Apr 13.

Department of Tumor Escape, Resistance and Immunity, TGF-Beta and Immuno-Regulation Team, Cancer Research Centre of Lyon (CRCL), INSERM U 1052, CNRS UMR 5286, UCBL1, Université de Lyon, Centre Léon Bérard, 28 rue Laennec, 69373, Lyon Cedex 08, France.

Mammalian cytosine DNA methylation (5mC) is associated with the integrity of the genome and the transcriptional status of nuclear DNA. Due to technical limitations, it has been less clear if mitochondrial DNA (mtDNA) is methylated and whether 5mC has a regulatory role in this context. Here, we used bisulfite-independent single-molecule sequencing of native human and mouse DNA to study mitochondrial 5mC across different biological conditions. Read More

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Humic + Fulvic acid mitigated Cd adverse effects on plant growth, physiology and biochemical properties of garden cress.

Sci Rep 2021 Apr 13;11(1):8040. Epub 2021 Apr 13.

Department of Agricultural Trade and Management, Yeditepe University, 34755, Istanbul, Turkey.

Cadmium (Cd) is a toxic and very mobile heavy metal that can be adsorbed and uptaken by plants in large quantities without any visible sign. Therefore, stabilization of Cd before uptake is crucial to the conservation of biodiversity and food safety. Owing to the high number of carboxyl and phenolic hydroxyl groups in their structure, humic substances form strong bonds with heavy metals which makes them perfect stabilizing agents. Read More

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Quantifying the Evolutionary Constraints and Potential of Hepatitis C Virus NS5A Protein.

mSystems 2021 Apr 13;6(2). Epub 2021 Apr 13.

Cancer Institute, ZJU-UCLA Joint Center for Medical Education and Research, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

RNA viruses, such as hepatitis C virus (HCV), influenza virus, and SARS-CoV-2, are notorious for their ability to evolve rapidly under selection in novel environments. It is known that the high mutation rate of RNA viruses can generate huge genetic diversity to facilitate viral adaptation. However, less attention has been paid to the underlying fitness landscape that represents the selection forces on viral genomes, especially under different selection conditions. Read More

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Stress-dependent activation of myosin in the heart requires thin filament activation and thick filament mechanosensing.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences and British Heart Foundation Centre of Research Excellence, King's College London, SE1 1UL London, United Kingdom

Myosin-based regulation in the heart muscle modulates the number of myosin motors available for interaction with calcium-regulated thin filaments, but the signaling pathways mediating the stronger contraction triggered by stretch between heartbeats or by phosphorylation of the myosin regulatory light chain (RLC) remain unclear. Here, we used RLC probes in demembranated cardiac trabeculae to investigate the molecular structural basis of these regulatory pathways. We show that in relaxed trabeculae at near-physiological temperature and filament lattice spacing, the RLC-lobe orientations are consistent with a subset of myosin motors being folded onto the filament surface in the interacting-heads motif seen in isolated filaments. Read More

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mRNA-Seq reveals the quorum sensing system luxS gene contributes to the environmental fitness of Streptococcus suis type 2.

BMC Microbiol 2021 Apr 13;21(1):111. Epub 2021 Apr 13.

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.

Background: Streptococcus suis type 2 (SS2) is an important zoonotic pathogen. We have previously reported the structure of LuxS protein and found that the luxS gene is closely related to biofilm, virulence gene expression and drug resistance of SS2. However, the mechanism of luxS mediated SS2 stress response is unclear. Read More

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The virtual physiological human gets nerves! How to account for the action of the nervous system in multiphysics simulations of human organs.

J R Soc Interface 2021 Apr 14;18(177):20201024. Epub 2021 Apr 14.

Centre for Computational Engineering Sciences, Cranfield University, Bedford MK43 0AL, UK.

This article shows how to couple multiphysics and artificial neural networks to design computer models of human organs that autonomously adapt their behaviour to environmental stimuli. The model simulates motility in the intestine and adjusts its contraction patterns to the physical properties of the luminal content. Multiphysics reproduces the solid mechanics of the intestinal membrane and the fluid mechanics of the luminal content; the artificial neural network replicates the activity of the enteric nervous system. Read More

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Costs of reproduction under experimental climate change across elevations in the perennial forb .

Proc Biol Sci 2021 Apr 14;288(1948):20203134. Epub 2021 Apr 14.

Department of Genetics and the Odum School of Ecology, University of Georgia, Athens, GA 30602, USA.

Investment in current reproduction can reduce future fitness by depleting resources needed for maintenance, particularly under environmental stress. These trade-offs influence life-history evolution. We tested whether climate change alters the future-fitness costs of current reproduction in a large-scale field experiment of (Brassicaceae). Read More

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Protocol for The Pregnancy During the COVID-19 Pandemic (PdP) study: A longitudinal cohort study of mental health among pregnant Canadians during the COVID-19 pandemic and developmental outcomes in their children.

JMIR Res Protoc 2021 Apr 13. Epub 2021 Apr 13.

University of Calgary, 2500 University Drive, NW, Calgary, CA.

Background: The COVID-19 pandemic and countermeasures implemented by governments around the world have led to dramatically increased symptoms of depression and anxiety. Pregnant women may be particularly vulnerable to the negative psychological effects of COVID-19 public health measures, because they represent a demographic that is most affected by disasters and because pregnancy itself entails significant life changes that require major psychosocial and emotional adjustments. The Pregnancy During the COVID-19 Pandemic (PdP) study was designed to investigate the associations between exposure to objective hardship caused by the pandemic, perceived stress and psychological distress in pregnant individuals, and developmental outcomes in their offspring. Read More

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Analysis of Populus glycosyl hydrolase family I members and their potential role in the ABA treatment and drought stress response.

Plant Physiol Biochem 2021 Apr 5;163:178-188. Epub 2021 Apr 5.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China. Electronic address:

Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs) are encoded by a large number of genes and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in the model plant species Arabidopsis thaliana, rice and maize but not in woody plant species, which have important economic and ecological value. In this study, we systematically analyzed Populus BGLUs (PtBGLUs) and demonstrated the involvement of several genes under stress conditions. Read More

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Statistical Learning and Prosodic Bootstrapping Differentially Affect Neural Synchronization during Speech Segmentation.

Neuroimage 2021 Apr 10:118051. Epub 2021 Apr 10.

Department of Cognition, Development and Educational Psychology, Campus Bellvitge, University of Barcelona, 5L'Hospitalet de Llobregat, 08097, Barcelona, Spain; Cognition and Brain Plasticity Group, Bellvitge Biomedical Research Institute, L'Hospitalet de Llobregat, 08097, Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats, ICREA, 08010, Barcelona, Spain. Electronic address:

Neural oscillations constitute an intrinsic property of functional brain organization that facilitates the tracking of linguistic units at multiple time scales through brain-to-stimulus alignment. This ubiquitous neural principle has been shown to facilitate speech segmentation and word learning based on statistical regularities. However, there is no common agreement yet on whether speech segmentation is mediated by a transition of neural synchronization from syllable to word rate, or whether the two time scales are concurrently tracked. Read More

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Tumor necrosis factor-alpha and interferon-gamma induce inflammasome-mediated corneal endothelial cell death.

Exp Eye Res 2021 Apr 10:108574. Epub 2021 Apr 10.

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Purpose: Chronic corneal endothelial cell (CEC) loss results in corneal edema and vision loss in conditions such as pseudophakic bullous keratopathy (PBK), Fuchs' dystrophy, and corneal graft failure. Low CEC density has been associated with an elevation of intraocular pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α and interferon (INF)-γ. These cytokines are capable of triggering pyroptosis, a programmed cell death mechanism mediated by the inflammasome, prompting the activation of the pro-inflammatory cytokine interleukin (IL)-1β, the perpetuation of inflammation, and subsequent damage of corneal endothelial tissue. Read More

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Effect of aromatherapy on autonomic nervous system regulation with treadmill exercise-induced stress among adolescents.

PLoS One 2021 13;16(4):e0249795. Epub 2021 Apr 13.

Department of Pediatric Emergency, Changhua Christian Children Hospital, Changhua, Taiwan.

Introduction: Stress is a major health issue in adolescents owing to the important transitions experienced during this period. Aromatherapy is an effective method for the reduction of stress in adolescents.

Purpose: The aims of this study were to examine the effect of aromatherapy on the regulation of the autonomic nervous system (ANS) along with stress relief and to explore the effect of aromatherapy on adolescents with different levels of stress. Read More

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Shotgun metagenomics reveals distinct functional diversity and metabolic capabilities between 12 000-year-old permafrost and active layers on Muot da Barba Peider (Swiss Alps).

Microb Genom 2021 Apr;7(4)

Forest Soils and Biogeochemistry, Swiss Federal Institute for Forest, Snow and Landscape Research WSL, Birmensdorf, Switzerland.

The warming-induced thawing of permafrost promotes microbial activity, often resulting in enhanced greenhouse gas emissions. The ability of permafrost microorganisms to survive the sub-zero temperatures, their energetic strategies and their metabolic versatility in using soil organic materials determine their growth and functionality upon thawing. Hence, functional characterization of the permafrost microbiome, particularly in the underexplored mid-latitudinal alpine regions, is a crucial first step in predicting its responses to the changing climate, and the consequences for soil-climate feedbacks. Read More

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Acetylated Ubiquitin Modulates the Catalytic Activity of the E1 Enzyme Uba1.

Biochemistry 2021 Apr 13. Epub 2021 Apr 13.

Department of Biochemistry, Western University, London, Ontario N6A 5C1, Canada.

Ubiquitin (Ub) signaling requires the covalent passage of Ub among E1, E2, and E3 enzymes. The choice of E2 and E3 enzymes combined with multiple rounds of the cascade leads to the formation of polyubiquitin chains linked through any one of the seven lysines on Ub. The linkage type and length act as a signal to trigger important cellular processes such as protein degradation or the DNA damage response. Read More

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Silicon induces metallochaperone-driven Cd binding to the cell wall and restores redox status through elevated glutathione in Cd-toxic sugar beet.

Physiol Plant 2021 Apr 13. Epub 2021 Apr 13.

Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Republic of Korea.

Cadmium (Cd) is toxic; however, whether silicon (Si) alleviates Cd toxicity was never studied in sugar beet. The study was conducted on two weeks old sugar beet cultivated in the presence or absence of Cd (10 μM CdSO ) and Si (1 mM Na SiO ) in hydroponic conditions. The morphological impairment and cellular damages observed in sugar beet upon Cd toxicity were entirely reversed due to Si. Read More

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Mitigating the stress response to improve outcomes for older patients undergoing emergency surgery with the addition of beta-adrenergic blockade.

Eur J Trauma Emerg Surg 2021 Apr 13. Epub 2021 Apr 13.

University of Southern California, Los Angeles, USA.

As population age, healthcare systems and providers are likely to experience a substantial increase in the proportion of elderly patients requiring emergency surgery. Emergency surgery, compared with planned surgery, is strongly associated with increased risks of adverse postoperative outcomes due to the short time available for diagnosis, optimization, and intervention in patients presenting with physiological derangement. These patient populations, who are often frail and burdened with a variety of co-morbidities, have lower reserves to deal with the stress of the acute condition and the required emergency surgical intervention. Read More

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Contraction-relaxation coupling is unaltered by exercise training and infarction in isolated canine myocardium.

J Gen Physiol 2021 Jul;153(7)

Department of Physiology and Cell Biology, College of Medicine, Ohio State University, Columbus, OH.

The two main phases of the mammalian cardiac cycle are contraction and relaxation; however, whether there is a connection between them in humans is not well understood. Routine exercise has been shown to improve cardiac function, morphology, and molecular signatures. Likewise, the acute and chronic changes that occur in the heart in response to injury, disease, and stress are well characterized, albeit not fully understood. Read More

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