3,031 results match your criteria streptomyces coelicolor

Biochemical and Mutational Analysis of Radical -Adenosyl-L-Methionine Adenosylhopane Synthase HpnH from Reveals that the Conserved Residue Cysteine-106 Reduces a Radical Intermediate and Determines the Stereochemistry.

Biochemistry 2021 Sep 10. Epub 2021 Sep 10.

Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan.

Adenosylhopane is a crucial precursor of C hopanoids, which are believed to modulate the fluidity and permeability of bacterial cell membranes. Adenosylhopane is formed by a crosslinking reaction between diploptene and a 5'-deoxyadenosyl radical that is generated by the radical -adenosyl-L-methionine (SAM) enzyme HpnH. We previously showed that HpnH from A3(2) (ScHpnH) converts diploptene to (22)-adenosylhopane. Read More

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September 2021

Expression of the Mycobacterium tuberculosis RipA cell-wall hydrolase in Streptomyces coelicolor hampers vancomycin resistance.

J Glob Antimicrob Resist 2021 Sep 4. Epub 2021 Sep 4.

Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Spain; Department of Functional Biology, Microbiology Area, Medical School, University of Oviedo, Spain; Centre for Bacterial Cell Biology, Medical School, Newcastle University, Newcastle Upon Tyne, UK. Electronic address:

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September 2021

Four-Step Pathway from Phenylpyruvate to Benzylamine, an Intermediate to the High-Energy Propellant CL-20.

ACS Synth Biol 2021 Sep 7;10(9):2187-2196. Epub 2021 Sep 7.

Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

Benzylamine is a commodity chemical used in the synthesis of motion-sickness treatments and anticonvulsants, in dyeing textiles, and as a precursor to the high-energy propellant CL-20. Because chemical production generates toxic waste streams, biosynthetic alternatives have been explored, recently resulting in a functional nine-step pathway from central metabolism (phenylalanine) in . We report a novel four-step pathway for benzylamine production, which generates the product from cellular phenylpyruvate using enzymes from different sources: a mandelate synthase (), a mandelate oxidase (), a benzoylformate decarboxylase (), and an aminotransferase (). Read More

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September 2021

Correction: Hardy et al. Genome Sequence and Characterization of Five Bacteriophages Infecting and : Alderaan, Coruscant, Dagobah, Endor1 and Endor2. 2020, , 1065.

Viruses 2021 Aug 16;13(8). Epub 2021 Aug 16.

Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425 Jülich, Germany.

The authors wish to make the following corrections to this paper [... Read More

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Establishing an efficient salinomycin biosynthetic pathway in three heterologous Streptomyces hosts by constructing a 106-kb multioperon artificial gene cluster.

Biotechnol Bioeng 2021 Aug 26. Epub 2021 Aug 26.

State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Helmholtz International Lab for Anti-infectives, Shandong University-Helmholtz Institute of Biotechnology, Shandong University, Qingdao, Shandong, China.

Salinomycin is a promising anticancer drug for chemotherapy. A highly productive biosynthetic gene cluster will facilitate the creation of analogs with improved therapeutic activity and reduced side effects. In this study, we engineered an artificial 106-kb salinomycin gene cluster and achieved efficient heterologous expression in three hosts: Streptomyces coelicolor CH999, S. Read More

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The Response Regulator MacR and its Potential in Improvement of Antibiotic Production in Streptomyces coelicolor.

Curr Microbiol 2021 Oct 23;78(10):3696-3707. Epub 2021 Aug 23.

The State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

We previously reported that the two-component system MacRS regulates morphogenesis and production of the blue-pigmented antibiotic actinorhodin (ACT) in Streptomyces coelicolor. In this study, the role of MacRS was further extended to include control of the production of the red-pigmented antibiotic undecylprodigiosin (RED) and the calcium-dependent antibiotic (CDA), and control of other important cellular activities. Our data indicated that disruption of the MacRS TCS reduced production not only of ACT but also of RED and CDA. Read More

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October 2021

Impact of MtrA on phosphate metabolism genes and the response to altered phosphate conditions in Streptomyces.

Environ Microbiol 2021 Aug 14. Epub 2021 Aug 14.

The State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Phosphate metabolism is known to be regulated by the PhoPR regulatory system in Streptomyces and some other bacteria. In this study, we report that MtrA also regulates phosphate metabolism in Streptomyces. Our data showed that, in Streptomyces coelicolor, MtrA regulates not only phosphate metabolism genes such as phoA but also phoP under different phosphate conditions, including growth on rich complex media without added inorganic phosphate and on defined media with low or high concentrations of inorganic phosphate. Read More

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Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region.

Turk J Biol 2021 23;45(3):275-286. Epub 2021 Jun 23.

Molecular Biology and Genetic Department, Faculty of Science, Gebze Technical University, Kocaeli Turkey.

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive A3(2) has been indicated as a high potential PHA producer, C gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. Read More

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SERR Spectroelectrochemistry as a Guide for Rational Design of DyP-Based Bioelectronics Devices.

Int J Mol Sci 2021 Jul 27;22(15). Epub 2021 Jul 27.

Instituto de Tecnologia Química e Biológica António Xavier, Universidade NOVA de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.

Immobilised dye-decolorizing peroxidases (DyPs) are promising biocatalysts for the development of biotechnological devices such as biosensors for the detection of HO. To this end, these enzymes have to preserve native, solution properties upon immobilisation on the electrode surface. In this work, DyPs from (CboDyP), (ScoDyP) and (TfuDyP) are immobilised on biocompatible silver electrodes functionalized with alkanethiols. Read More

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Cross-Recognition of Promoters by the Nine SigB Homologues Present in A3(2).

Int J Mol Sci 2021 Jul 22;22(15). Epub 2021 Jul 22.

Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovakia.

In contrast to , A3(2) contains nine homologues of stress response sigma factor SigB with a major role in differentiation and osmotic stress response. The aim of this study was to further characterize these SigB homologues. We previously established a two-plasmid system to identify promoters recognized by sigma factors and used it to identify promoters recognized by the three SigB homologues, SigF, SigG, and SigH from A3(2). Read More

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Protein synthesis in Mycobacterium tuberculosis as a potential target for therapeutic interventions.

Mol Aspects Med 2021 Jul 31:101002. Epub 2021 Jul 31.

Structural Biology & Translation Regulation Laboratory, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, 121 001, India. Electronic address:

Mycobacterium tuberculosis (Mtb) causes one of humankind's deadliest diseases, tuberculosis. Mtb protein synthesis machinery possesses several unique species-specific features, including its ribosome that carries two mycobacterial specific ribosomal proteins, bL37 and bS22, and ribosomal RNA segments. Since the protein synthesis is a vital cellular process that occurs on the ribosome, a detailed knowledge of the structure and function of mycobacterial ribosomes is essential to understand the cell's proteome by translation regulation. Read More

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Structures of full-length VanR from Streptomyces coelicolor in both the inactive and activated states.

Acta Crystallogr D Struct Biol 2021 Aug 29;77(Pt 8):1027-1039. Epub 2021 Jul 29.

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, USA.

Vancomycin has historically been used as a last-resort treatment for serious bacterial infections. However, vancomycin resistance has become widespread in certain pathogens, presenting a serious threat to public health. Resistance to vancomycin is conferred by a suite of resistance genes, the expression of which is controlled by the VanR-VanS two-component system. Read More

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Identification of the cognate response regulator of the orphan histidine kinase OhkA involved in both secondary metabolism and morphological differentiation in Streptomyces coelicolor.

Appl Microbiol Biotechnol 2021 Aug 21;105(14-15):5905-5914. Epub 2021 Jul 21.

Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences (CAS), Shanghai, 200032, China.

In the model actinomycete strain, Streptomyces coelicolor, an orphan histidine kinase (HK) named OhkA (encoded by SCO1596), which belongs to bacterial two-component regulatory systems (TCSs), has been identified as being involved in the regulation of both antibiotic biosynthesis and morphological development. However, its cognate response regulator (RR) remains unknown due to its isolated genetic location on the genome, which impedes the elucidation of the mechanism underlying OhkA-mediated regulation. Here, we identified the orphan RR OrrA (encoded by SCO3008) as the cognate RR of OhkA according to mutant phenotypic changes, transcriptomics analysis, and bacterial two-hybrid experiment. Read More

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Effects of and on Growth Performance of Broiler Chickens.

Microorganisms 2021 Jun 21;9(6). Epub 2021 Jun 21.

Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, TN 37209, USA.

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of () and Actinobacteria class of bacteria in their gastrointestinal tract. Read More

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Directed evolution of alditol oxidase for the production of optically pure D-glycerate from glycerol in the engineered Escherichia coli.

J Ind Microbiol Biotechnol 2021 Aug;48(7-8)

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 Xiqidao, Tianjin Airport Economic Park, Tianjin 300308, China.

D-glycerate is an attractive chemical for a wide variety of pharmaceutical, cosmetic, biodegradable polymers, and other applications. Now several studies have been reported about the synthesis of glycerate by different biotechnological and chemical routes from glycerol or other feedstock. Here, we present the construction of an Escherichia coli engineered strain to produce optically pure D-glycerate by oxidizing glycerol with an evolved variant of alditol oxidase (AldO) from Streptomyces coelicolor. Read More

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Enzymatic treatment of phenolic pollutants by a small laccase immobilized on APTES-functionalised magnetic nanoparticles.

3 Biotech 2021 Jun 30;11(6):302. Epub 2021 May 30.

Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P.O. BOX 1334, Durban, 4000 South Africa.

In this study, we have successfully synthesized magnetic nanoparticles (MNPs), functionalised them by silanization and used them for the covalent immobilization of a recombinant small laccase (rSLAC) from . The immobilized recombinant laccase (MNP-rSLAC) was subsequently used for the treatment of phenol, 4-chlorophenol (4-CP) and 4-fluorophenol (4-FP). The enzyme completely degraded 80 µg/mL of the selected phenolic compounds within 2 h in the presence of a natural mediator, acetosyringone. Read More

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Stereoselective synthesis of a 4-⍺-glucoside of valienamine and its X-ray structure in complex with Streptomyces coelicolor GlgE1-V279S.

Sci Rep 2021 Jun 28;11(1):13413. Epub 2021 Jun 28.

Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH, 43606, USA.

Glycoside hydrolases (GH) are a large family of hydrolytic enzymes found in all domains of life. As such, they control a plethora of normal and pathogenic biological functions. Thus, understanding selective inhibition of GH enzymes at the atomic level can lead to the identification of new classes of therapeutics. Read More

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Bioremediation of lindane contaminated soil: Exploring the potential of actinobacterial strains.

Chemosphere 2021 Sep 3;278:130468. Epub 2021 Apr 3.

Laboratory of Structural Biology and Lignin Biochemistry, Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618, Tallinn, Estonia. Electronic address:

Lindane, an organochlorine pesticide, causes detrimental impacts on the environment and human health owing to its high toxicity, low degradation, and bioaccumulation. Its toxic nature can be overcome by biological and eco-friendly approaches involving its degradation and detoxification. The biodegradation of lindane was assessed using actinobacterial species Thermobifida cellulosilytica TB100 (T. Read More

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September 2021

Pleiotropic effects of ActVI-ORFA as an unusual regulatory factor identified in the biosynthetic pathway of actinorhodin in Streptomyces coelicolor.

Microbiol Res 2021 Sep 27;250:126792. Epub 2021 May 27.

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, PR China; The College of Life Sciences, Central China Normal University, Wuhan, 430079, PR China. Electronic address:

Regulatory networks play critical roles in controlling the the biosynthesis of natural products in Streptomyces. ActVI-ORFA, a regulatory factor encoded by the actinorhodin biosynthetic gene cluster (act cluster), positively controls the production of actinorhodin (ACT) in Streptomyces coelicolor, although its regulatory mechanism remains obscure. This study aimed to identify the regulatory targets of ActVI-ORFA. Read More

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September 2021

Fluorescent labeling in size-controlled liposomes reveals membrane curvature-induced structural changes in the KcsA potassium channel.

FEBS Lett 2021 07 23;595(14):1914-1919. Epub 2021 Jun 23.

Department of Molecular Neuroscience, Faculty of Medical Sciences, University of Fukui, Japan.

Biological structures with highly curved membranes, such as caveolae and transport vesicles, are essential for signal transduction and membrane trafficking. Although membrane proteins in these structures are subjected to physical stress due to the curvature of the lipid bilayers, the effect of this membrane curvature on protein structure and function remains unclear. In this study, we established an experimental procedure to evaluate membrane curvature-induced structural changes in the prototypical potassium channel KcsA. Read More

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Reconstruction of a Genome-Scale Metabolic Model of J1074: Improved Engineering Strategies in Natural Product Synthesis.

Metabolites 2021 May 11;11(5). Epub 2021 May 11.

Department of Biology and Biological Engineering, Chalmers University of Technology, 41296 Gothenburg, Sweden.

J1074 is recognized as an effective host for heterologous production of natural products. Its fast growth and efficient genetic toolbox due to a naturally minimized genome have contributed towards its advantage in expressing biosynthetic pathways for a diverse repertoire of products such as antibiotics and flavonoids. In order to develop precise model-driven engineering strategies for de novo production of natural products, a genome-scale metabolic model (GEM) was reconstructed for the microorganism based on protein homology to model species   while drawing annotated data from databases and literature for further curation. Read More

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Poly- and Monoamine Metabolism in Streptomyces coelicolor: The New Role of Glutamine Synthetase-Like Enzymes in the Survival under Environmental Stress.

Microb Physiol 2021 May 27:1-15. Epub 2021 May 27.

Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), Cluster of Excellence 'Controlling Microbes to Fight Infections', University of Tübingen, Tübingen, Germany.

Soil bacteria from the genus Streptomyces, phylum Actinobacteria, feature a complex metabolism and diverse adaptations to environmental stress. These characteristics are consequences of variable nutrition availability in the soil and allow survival under changing nitrogen conditions. Streptomyces coelicolor is a model organism for Actinobacteria and is able to use nitrogen from a variety of sources including unusual compounds originating from the decomposition of dead plant and animal material, such as polyamines or monoamines (like ethanolamine). Read More

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Streptomyces consortia-mediated plant defense against Fusarium wilt and plant growth-promotion in chickpea.

Microb Pathog 2021 Aug 24;157:104961. Epub 2021 May 24.

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Telangana, India. Electronic address:

Three strains of Streptomyces griseus (CAI-24, CAI-121 and CAI-127) and one strain each of Streptomyces africanus (KAI-32) and Streptomyces coelicolor (KAI-90) were reported by us as biocontrol agents against Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceri (FOC), and as plant growth-promoters (PGP) in chickpea. Read More

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Genetic Network Architecture and Environmental Cues Drive Spatial Organization of Phenotypic Division of Labor in Streptomyces coelicolor.

mBio 2021 05 18;12(3). Epub 2021 May 18.

Department of Plant and Microbial Biology, University of California, Berkeley, California, USA

A number of bacteria are known to differentiate into cells with distinct phenotypic traits during processes such as biofilm formation or the development of reproductive structures. These cell types, by virtue of their specialized functions, embody a division of labor. However, how bacteria build spatial patterns of differentiated cells is not well understood. Read More

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Nitric Oxide Signaling for Actinorhodin Production in Streptomyces coelicolor A3(2) via the DevS/R Two-Component System.

Appl Environ Microbiol 2021 06 25;87(14):e0048021. Epub 2021 Jun 25.

Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.

Nitric oxide (NO) is an important signaling molecule in eukaryotic and prokaryotic cells. A previous study revealed an NO synthase-independent NO production metabolic cycle in which the three nitrogen oxides, nitrate (NO), nitrite (NO), and NO, were generated in the actinobacterium Streptomyces coelicolor A3(2). NO was suggested to act as a signaling molecule, functioning as a hormone that regulates secondary metabolism. Read More

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Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.

Microb Cell Fact 2021 May 13;20(1):99. Epub 2021 May 13.

Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.

Background: Identifying the regulatory factors that control transcriptional activity is a major challenge of gene expression studies. Here, we describe the application of a novel approach for in vivo identification of regulatory proteins that may directly or indirectly control the transcription of a promoter of interest in Streptomyces.

Results: A method based on the combination of Tn5 minitransposon-driven random mutagenesis and lux reporter genes was applied for the first time for the Streptomyces genus. Read More

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Spatial control of gene expression in flies using bacterially derived binary transactivation systems.

Insect Mol Biol 2021 Oct 24;30(5):461-471. Epub 2021 May 24.

Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, California, USA.

Controlling gene expression is an instrumental tool for biotechnology, as it enables the dissection of gene function, affording precise spatial-temporal resolution. To generate this control, binary transactivational systems have been used employing a modular activator consisting of a DNA binding domain(s) fused to activation domain(s). For fly genetics, many binary transactivational systems have been exploited in vivo; however, as the study of complex problems often requires multiple systems that can be used in parallel, there is a need to identify additional bipartite genetic systems. Read More

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October 2021

Redox-sensitive transcriptional regulator SoxR directly controls antibiotic production, development and thiol-oxidative stress response in Streptomyces avermitilis.

Microb Biotechnol 2021 May 5. Epub 2021 May 5.

State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, China.

The redox-sensitive transcriptional regulator SoxR is conserved in bacteria. Its role in mediating protective response to various oxidative stresses in Escherichia coli and related enteric bacteria has been well established. However, functions and regulatory mechanisms of SoxR in filamentous Streptomyces, which produce half of known antibiotics, are unclear. Read More

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The ARC2 response in requires the global regulatory genes and .

Microbiology (Reading) 2021 05;167(5)

Department of Biochemistry University of Toronto MaRS Discovery District 661 University Avenue Toronto, Ontario CANADA M5G 1M1, Canada.

ARC2 is a synthetic compound, related in structure and mechanism to the antibiotic triclosan, that activates the production of many specialized metabolites in the genus of bacteria. In this work, we demonstrate that the addition of ARC2 to cultures results in considerable alterations in overall gene expression including most notably the specialized metabolic genes. Using actinorhodin production as a model system, we show that the effect of ARC2 depends on the pleiotropic regulators and but not . Read More

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