1,538 results match your criteria strategy manipulate

Mechanisms adopted by Salmonella to colonize plant hosts.

Food Microbiol 2021 Oct 20;99:103833. Epub 2021 May 20.

Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11/12, 38104, Braunschweig, Germany. Electronic address:

Fruits and vegetables consumed fresh or as minimally-processed produce, have multiple benefits for our diet. Unfortunately, they bring a risk of food-borne diseases, for example salmonellosis. Interactions between Salmonella and crop plants are indeed a raising concern for the global health. Read More

View Article and Full-Text PDF
October 2021

How to Manipulate Through-Space Conjugation and Clusteroluminescence of Simple AIEgens with Isolated Phenyl Rings.

J Am Chem Soc 2021 Jun 11. Epub 2021 Jun 11.

Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction and Institute for Advanced Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China.

Apart from the traditional through-bond conjugation (TBC), through-space conjugation (TSC) is gradually proved as another important interaction in photophysical processes, especially for the recent observation of clusteroluminescence from nonconjugated molecules. However, unlike TBC in conjugated chromophores, it is still challenging to manipulate TSC and clusteroluminescence. Herein, simple and nonconjugated triphenylmethane (TPM) and its derivatives with electron-donating and electron-withdrawing groups were synthesized, and their photophysical properties were systematically studied. Read More

View Article and Full-Text PDF

Root mass carbon costs to acquire nitrogen are determined by nitrogen and light availability in two species with different nitrogen acquisition strategies.

J Exp Bot 2021 Jun 11. Epub 2021 Jun 11.

Department of Biological Sciences, Texas Tech University, Lubbock, TX USA.

Plant nitrogen acquisition requires carbon to be allocated belowground to build roots and sustain microbial associations. This carbon cost to acquire nitrogen varies by nitrogen acquisition strategy; however, the degree to which these costs vary due to nitrogen availability or demand has not been well tested under controlled conditions. We grew a species capable of forming associations with nitrogen-fixing bacteria (Glycine max) and a species not capable of forming such associations (Gossypium hirsutum) under four soil nitrogen levels to manipulate nitrogen availability and four light levels to manipulate nitrogen demand in a full-factorial greenhouse experiment. Read More

View Article and Full-Text PDF

High-Performance Indium Gallium Tin Oxide Transistors with an AlO Gate Insulator Deposited by Atomic Layer Deposition at a Low Temperature of 150 °C: Roles of Hydrogen and Excess Oxygen in the AlO Dielectric Film.

ACS Appl Mater Interfaces 2021 Jun 11. Epub 2021 Jun 11.

Department of Electronic Engineering, Hanyang University, Seoul 04763, South Korea.

In this work, high-performance amorphous InGaSnO (-IGTO) transistors with an atomic layer-deposited AlO dielectric layer were fabricated at a maximum processing temperature of 150 °C. Hydrogen (H) and excess oxygen (O) in the AlO film, which was controlled by adjusting the oxygen radical density (P: flow rate of O/[Ar+O]) in the radio-frequency (rf) plasma during ALD growth of AlO, significantly affected the performance and stability of the resulting IGTO transistors. The concentrations of H and O in AlO/IGTO stacks according to P were characterized by secondary ion mass spectroscopy, X-ray photoelectron spectroscopy, hard X-ray photoemission spectroscopy, and thermal desorption spectroscopy. Read More

View Article and Full-Text PDF

Effects of Gene Delivery Approaches on Differentiation Potential and Gene Function of Mesenchymal Stem Cells.

IEEE Trans Biomed Eng 2021 Jun 8;PP. Epub 2021 Jun 8.

Introduction of a gene to mesenchymal stem cells (MSCs) is a well-known strategy to purposely manipulate the cell fate and further enhance therapeutic performance in cell-based therapy. Viral and chemical approaches for gene delivery interfere with differentiation potential. Although microinjection as a physical delivery method is commonly used for transfection, its influence on MSC cell fate is not fully understood. Read More

View Article and Full-Text PDF

Enhanced osteogenic differentiation of mesenchymal stem cells on P(VDF-TrFE) layer coated microelectrodes.

J Biomed Mater Res B Appl Biomater 2021 Jun 3. Epub 2021 Jun 3.

School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Zhejiang, China.

Electrical stimulation has been proved to be critical to regulate cell behavior. But, cell behavior is also susceptible to multiple parameters of the adverse interferences such as surface current, electrochemical reaction products, and non-uniform compositions, which often occur during direct electric stimulation. To effectively prevent the adverse interferences, a novel piezoelectric poly(vinylidene fluoride-trfluoroethylene)(P(VDF-TrFE)) layer was designed to coat onto the indium tin oxide (ITO) planar microelectrode. Read More

View Article and Full-Text PDF

Communal living: the role of polyploidy and syncytia in tissue biology.

Chromosome Res 2021 Jun 1. Epub 2021 Jun 1.

Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA.

Multicellular organisms are composed of tissues with diverse cell sizes. Whether a tissue primarily consists of numerous, small cells as opposed to fewer, large cells can impact tissue development and function. The addition of nuclear genome copies within a common cytoplasm is a recurring strategy to manipulate cellular size within a tissue. Read More

View Article and Full-Text PDF

Switchable Binding Energy of Ionic Compounds and Application in Customizable Ligand Exchange for Colloid Nanocrystals.

J Phys Chem Lett 2021 Jun 1;12(22):5271-5278. Epub 2021 Jun 1.

Key Laboratory for Physical Electronics and Devices of the Ministry of Education, School of Electronic Science and Engineering, Faculty of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an, Shannxi 710049, P. R. China.

The ability to engineer the surface ligands or adsorbed molecules on colloid nanocrystals (NCs) is important for various applications, as the physical and chemical properties are strongly affected by the surface chemistry. Here, we develop a facile and generalized ionic compound-mediated ligand-exchange strategy based on density functional theory calculations, in which the ionic compounds possess switchable bonding energy when they transfer between the ionized state and the non-ionized state, hence catalyzing the ligand-exchange process. By using an organic acid as the intermediate ligand, ligands such as oleylamine, butylamine, polyvinylpyrrolidone, and poly(vinyl alcohol) can be freely exchanged on the surface of Au NCs. Read More

View Article and Full-Text PDF

Simple Reciprocal Fairness Message to Enhance Non-Donor's Willingness to Donate Blood.

Ann Behav Med 2021 May 29. Epub 2021 May 29.

School of Psychology, The University of Queensland, St Lucia 4067, Australia.

Background: Against a background of declining blood donor numbers, recruiting new donors is critical for the effective operations of healthcare providers. Thus, interventions are needed to recruit new blood donors.

Purpose: We provide initial evidence for Voluntary Reciprocal Altruism (VRA) to enhance nondonors' willingness to become blood donors. Read More

View Article and Full-Text PDF

Exploring the Influence of Different Albumin Binders on Molecular Imaging Probe Distribution.

Mol Pharm 2021 May 28. Epub 2021 May 28.

Clinic for Radiology, University Hospital Münster, 48149 Münster, Germany.

The biodistribution of molecular imaging probes or tracers mainly depends on the chemical nature of the probe and the preferred metabolization and excretion routes. Small molecules have rather short half-lives while antibodies reside inside the organism for a longer period of time. An excretion via kidneys and bladder is faster than a mainly hepatobiliary elimination. Read More

View Article and Full-Text PDF

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.

Sci China Life Sci 2021 May 27. Epub 2021 May 27.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.

Viruses utilize cellular lipids and manipulate host lipid metabolism to ensure their replication and spread. Therefore, the identification of lipids and metabolic pathways that are suitable targets for antiviral development is crucial. Using a library of compounds targeting host lipid metabolic factors and testing them for their ability to block pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) infection, we found that U18666A, a specific inhibitor of Niemann-Pick C1 (NPC1), is highly potent in suppressing the entry of diverse viruses including pseudotyped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Read More

View Article and Full-Text PDF

Serotonin neurons in the median raphe nucleus bidirectionally regulate somatic signs of nicotine withdrawal in mice.

Biochem Biophys Res Commun 2021 Jul 24;562:62-68. Epub 2021 May 24.

Department of Neuropharmacology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, N15 W7 Kita-ku, Sapporo, 060-8638, Japan.

In chronic smokers, nicotine withdrawal symptoms during tobacco cessation can lead to smoking relapse. In rodent models, chronic exposure to nicotine elicited physical dependence, whereas acute antagonism of nicotinic acetylcholine receptors (nAChRs) immediately precipitated withdrawal symptoms. Although the central serotonergic system plays an important role in nicotine withdrawal, the exact serotonergic raphe nuclei regulating these symptoms remain unknown. Read More

View Article and Full-Text PDF

Mesoporogen-Free Strategy to Construct Hierarchical TS-1 in a Highly Concentrated System for Gas-Phase Propene Epoxidation with H and O.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):26134-26142. Epub 2021 May 26.

State Key Lab of Heavy Oil Processing, China University of Petroleum, Qingdao 266580, China.

Hierarchical TS-1 has attracted enormous attention from both academia and industry due to its remarkable catalytic performance in epoxidation reactions. However, sustainable synthesis of hierarchical-nanosized TS-1 without mesoporogens is still challenging. In this work, we report a facile and mesoporogen-free strategy to simultaneously manipulate pore structure and particle size of TS-1 employing the concentrated system. Read More

View Article and Full-Text PDF

Regulating the Aggregation of Unfused Non-Fullerene Acceptors via Molecular Engineering towards Efficient Polymer Solar Cells.

ChemSusChem 2021 May 26. Epub 2021 May 26.

Department of Chemistry, City University of Hong Kong, Kowloon, 999077, Hong Kong, P. R. China.

Tuning molecular aggregation via structure design to manipulate the film morphology still remains as a challenge for polymer solar cells based on unfused non-fullerene acceptors (UF-NFAs). Herein, a strategy was developed to modulate the aggregation patterns of UF-NFAs by systematically varying the π-bridge (D) unit and central core (A') unit in A-D-A'-D-A framework (A and D refer to electron-withdrawing and electron-donating moieties, respectively). Specifically, the quantified contents of H- or J-aggregation and crystallite disorder of three UF-NFAs (BDIC2F, BCIC2F, and TCIC2F) were analyzed via UV/Vis spectrometry and grazing incidence X-ray scattering. Read More

View Article and Full-Text PDF

Caged Cumate Enables Proximity-Dependent Control Over Gene Expression.

Chembiochem 2021 May 24. Epub 2021 May 24.

Department of Chemistry, University of California, Irvine, 1120 Natural Sciences II, Irvine, CA 92697, USA.

Cell-cell interactions underlie diverse physiological processes yet remain challenging to examine with conventional imaging tools. Here we report a novel strategy to illuminate cell proximity using transcriptional activators. We repurposed cumate, a small molecule inducer of gene expression, by caging its key carboxylate group with a nitrile. Read More

View Article and Full-Text PDF

Bottom-Up Assembled Photonic Crystals for Structure-Enabled Label-Free Sensing.

ACS Nano 2021 May 24. Epub 2021 May 24.

BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre & Max Planck Center for Complex Fluid Dynamics, University of Twente, 7522 NB Enschede, The Netherlands.

Photonic crystals (PhCs) display photonic stop bands (PSBs) and at the edges of these PSBs transport light with reduced velocity, enabling the PhCs to confine and manipulate incident light with enhanced light-matter interaction. Intense research has been devoted to leveraging the optical properties of PhCs for the development of optical sensors for bioassays, diagnosis, and environmental monitoring. These applications have furthermore benefited from the inherently large surface area of PhCs, giving rise to high analyte adsorption and the wide range of options for structural variations of the PhCs leading to enhanced light-matter interaction. Read More

View Article and Full-Text PDF

Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives.

Adv Biol (Weinh) 2021 May 14;5(5):e2000180. Epub 2021 Jan 14.

600 South Mathews Avenue, 314 B Roger Adams Laboratory, Urbana, IL, 61801, USA.

Optogenetics utilizes photosensitive proteins to manipulate the localization and interaction of molecules in living cells. Because light can be rapidly switched and conveniently confined to the sub-micrometer scale, optogenetics allows for controlling cellular events with an unprecedented resolution in time and space. The past decade has witnessed an enormous progress in the field of optogenetics within the biological sciences. Read More

View Article and Full-Text PDF

Modulating T Follicular Cells In Vivo Enhances Antigen-Specific Humoral Immunity.

J Immunol 2021 Jun 19;206(11):2583-2595. Epub 2021 May 19.

Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Harvard University, Boston, MA;

Generation of high-affinity IgG is essential for defense against infections and cancer, which is the intended consequence of many vaccines, but can cause autoimmune and inflammatory diseases when inappropriately directed against self. The interplay of T follicular helper (T) cells and T follicular regulatory (T) cells is critical for the production of high-affinity IgG of a specific subclass. In this study, we sought to improve Ag-specific IgG responses with two interventions intended to transiently diminish T cell influence. Read More

View Article and Full-Text PDF

Construction of Interlayer Conjugated Links in 2D Covalent Organic Frameworks via Topological Polymerization.

J Am Chem Soc 2021 Jun 19;143(21):7897-7902. Epub 2021 May 19.

Frontiers Science Center for High Energy Material, Advanced Technology Research Institute (Jinan), Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.

Two-dimensional covalent organic frameworks (2D COFs) are well-defined polymeric sheets that usually stack in an eclipsed mode via van der Waals forces. Extensive efforts have been made to manipulate interlayer interactions, yet there still lack a way to construct conjugated connections between adjacent layers, which is important for (opto)electronic-related applications. Herein, we report an interlayer topological polymerization strategy to transform the well-organized diacetylene columnar arrays in three different 2D COFs (TAPFY-COF, TAPB-COF, and TAPP-COF) into conjugated enyne chains upon heating in the solid state. Read More

View Article and Full-Text PDF

Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes.

Bioact Mater 2021 Nov 21;6(11):4065-4072. Epub 2021 Apr 21.

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Due to the critical roles of macrophage in immune response and tissue repair, harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many attentions. Although there have developed many advanced platforms with dynamic features for cell manipulation, few studies have designed a dynamic chemical pattern to sequentially polarize macrophage phenotypes and meet the immune requirements at various tissue repair stages. Here, we propose a novel strategy for spatiotemporal manipulation of macrophage phenotypes by a UV-induced dynamic Arg-Gly-Asp (RGD) pattern. Read More

View Article and Full-Text PDF
November 2021

Excited state character of Cibalackrot-type compounds interpreted in terms of Hückel-aromaticity: a rationale for singlet fission chromophore design.

Chem Sci 2021 Mar 25;12(17):6159-6171. Epub 2021 Mar 25.

Department of Chemistry - Ångström Laboratory, Uppsala University 751 20 Uppsala Sweden

The exact energies of the lowest singlet and triplet excited states in organic chromophores are crucial to their performance in optoelectronic devices. The possibility of utilizing singlet fission to enhance the performance of photovoltaic devices has resulted in a wide demand for tuneable, stable organic chromophores with wide S-T energy gaps (>1 eV). Cibalackrot-type compounds were recently considered to have favorably positioned excited state energies for singlet fission, and they were found to have a degree of aromaticity in the lowest triplet excited state (T). Read More

View Article and Full-Text PDF

Hydrogel-based therapeutic angiogenesis: An alternative treatment strategy for critical limb ischemia.

Biomaterials 2021 Jul 7;274:120872. Epub 2021 May 7.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, PR China. Electronic address:

Critical limb ischemia (CLI) is the most severe clinical manifestation of peripheral arterial disease (PAD), resulting in the total or partial loss of limb function. Although the conventional treatment strategy of CLI (e.g. Read More

View Article and Full-Text PDF

Who listens to mother? A whole-family perspective on the evolution of maternal hormone allocation.

Biol Rev Camb Philos Soc 2021 May 14. Epub 2021 May 14.

Behavioural Biology, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Nijenborgh 7, Groningen, 9747AG, The Netherlands.

Maternal effects, or the influence of maternal environment and phenotype on offspring phenotype, may allow mothers to fine-tune their offspring's developmental trajectory and resulting phenotype sometimes long after the offspring has reached independence. However, maternal effects on offspring phenotype do not evolve in isolation, but rather within the context of a family unit, where the separate and often conflicting evolutionary interests of mothers, fathers and offspring are all at play. While intrafamilial conflicts are routinely invoked to explain other components of reproductive strategy, remarkably little is known about how intrafamilial conflicts influence maternal effects. Read More

View Article and Full-Text PDF

Skeletal editing through direct nitrogen deletion of secondary amines.

Nature 2021 May 12;593(7858):223-227. Epub 2021 May 12.

Department of Chemistry, University of Chicago, Chicago, IL, USA.

Synthetic chemistry aims to build up molecular complexity from simple feedstocks. However, the ability to exert precise changes that manipulate the connectivity of the molecular skeleton itself remains limited, despite possessing substantial potential to expand the accessible chemical space. Here we report a reaction that 'deletes' nitrogen from organic molecules. Read More

View Article and Full-Text PDF

Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species.

Methods Mol Biol 2021 ;2296:91-141

Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Bacteria of the genus Streptomyces are one of the most important producers of biologically active natural products. Recent robust genomic sequencing of Streptomyces strains has shown enormous genetic potential for new natural products. However, many biosynthetic gene clusters are silent. Read More

View Article and Full-Text PDF
January 2021

Efficient electrotransformation of Rhodococcus ruber YYL with abundant extracellular polymeric substances via a cell wall-weakening strategy.

FEMS Microbiol Lett 2021 May;368(9)

MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.

Rhodococcus spp. have broad potential applications related to the degradation of organic contaminants and the transformation or synthesis of useful compounds. However, some Gram-positive bacteria are difficult to manipulate genetically due to low transformation efficiency. Read More

View Article and Full-Text PDF

Oncogenic WIP1 phosphatase attenuates the DNA damage response and sensitizes p53 mutant Jurkat cells to apoptosis.

Oncol Lett 2021 Jun 19;21(6):479. Epub 2021 Apr 19.

Department of Medical Biology, Institute of Health Sciences, University of Aydin Adnan Menderes, 09010 Aydin, Turkey.

Wild-type (wt) p53-induced phosphatase 1 (Wip1), encoded by the protein phosphatase, Mg/Mn dependent 1D (PPM1D) gene, is a serine/threonine phosphatase induced upon genotoxic stress in a p53-dependent manner. Wip1/PPM1D is frequently overexpressed, amplified and mutated in human solid tumors harboring wt p53 and is thus currently recognized as an oncogene. Oncogenic Wip1 dampens cellular stress responses, such as cell cycle checkpoints, apoptosis and senescence, and consequently increases resistance to anticancer therapeutics. Read More

View Article and Full-Text PDF

Modulating surface charge of dexamethasone non-spherical microcrystals for improved inner ear delivery.

Colloids Surf B Biointerfaces 2021 Apr 29;204:111806. Epub 2021 Apr 29.

Biomedical Barriers Research Center, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, 300192, China. Electronic address:

It is important to achieve precise surface charge manipulation of non-spherical drug microcrystals using facile and time-efficient methods for local drug delivery. In this study, silk-coated dexamethasone (DEX) non-spherical microcrystals were synthesized by precipitation technique followed by alternate deposition of poly(allylamine hydrochloride) (PAH) (or PAH-coated FeO) and silk fibroin (SF) via layer-by-layer assembly. EDC and glutaraldehyde were employed to manipulate positive or negative charge of particles by simple chemical cross-linking reactions, respectively. Read More

View Article and Full-Text PDF

A Quorum Sensing System Enables Suppression of Innate Immunity.

mBio 2021 05 4;12(3). Epub 2021 May 4.

Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, USA

Some bacterial pathogens utilize cell-cell communication systems, such as quorum sensing (QS), to coordinate genetic programs during host colonization and infection. The human-restricted pathosymbiont (group A streptococcus [GAS]) uses the Rgg2/Rgg3 QS system to modify the bacterial surface, enabling biofilm formation and lysozyme resistance. Here, we demonstrate that innate immune cell responses to GAS are substantially altered by the QS status of the bacteria. Read More

View Article and Full-Text PDF

Targeting S1PRs as a Therapeutic Strategy for Inflammatory Bone Loss Diseases-Beyond Regulating S1P Signaling.

Hong Yu

Int J Mol Sci 2021 Apr 23;22(9). Epub 2021 Apr 23.

Department of Oral Health Sciences, College of Dental Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.

As G protein coupled receptors, sphingosine-1-phosphate receptors (S1PRs) have recently gained attention for their role in modulating inflammatory bone loss diseases. Notably, in murine studies inhibiting S1PR2 by its specific inhibitor, JTE013, alleviated osteoporosis induced by RANKL and attenuated periodontal alveolar bone loss induced by oral bacterial inflammation. Treatment with a multiple S1PRs modulator, FTY720, also suppressed ovariectomy-induced osteoporosis, collagen or adjuvant-induced arthritis, and apical periodontitis in mice. Read More

View Article and Full-Text PDF