21,583 results match your criteria strains appeared


Advancement of chimeric hybrid drugs to cure malaria infection: An overview with special emphasis on endoperoxide pharmacophores.

Eur J Med Chem 2021 Apr 4;219:113408. Epub 2021 Apr 4.

Chemical Biology Laboratory, Department of Chemistry, University of Delhi, Delhi 110007, India. Electronic address:

Emergence and spread of Plasmodium falciparum resistant to artemisinin-based combination therapy has led to a situation of haste in the scientific and pharmaceutical communities. Sincere efforts are redirected towards finding alternative chemotherapeutic agents that are capable of combating multidrug-resistant parasite strains. Extensive research yielded the concept of "Chimeric Bitherapy (CB)" which involves the linking of two molecules with individual pharmacological activity and exhibit dual mode of action into a single hybrid molecule. Read More

View Article and Full-Text PDF

Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli.

Environ Res 2021 May 11:111321. Epub 2021 May 11.

Department of Biotechnology, Modern College of Arts, Science and Commerce (Savitribai Phule Pune University), Ganeshkhind, Pune, 411016, India; Department of Environmental Science, Savitribai Phule Pune University, Pune, 411007, India. Electronic address:

A global upsurge in emergence and spread of antibiotic resistance (ABR) in bacterial populations is a serious threat for human health. Unfortunately, ABR is no longer confined to nosocomial environments and is frequently reported from community microbes as well. The ABR is resulting in shrinking potent antibiotics pool and thus necessitating novel and alternative therapies and therapeutics. Read More

View Article and Full-Text PDF

Biosynthesis of silver nano-drug using and its synergistic antibacterial activity against multidrug-resistant bacteria for wound dressing applications.

3 Biotech 2021 Jun 8;11(6):255. Epub 2021 May 8.

Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif, 21944 Saudi Arabia.

We report here the synthesis of silver nanoparticles (AgNPs) from an aqueous extract of and their use as an antimicrobial agent on their own or in combination with antibiotics in inhibiting multidrug-resistant bacteria (MDR). One strategy of bacterial infection control in wound healing is AgNP biosynthesis. We collected bacterial strains of patient skin infections from Al-Adwani Hospital. Read More

View Article and Full-Text PDF

Impact of prior vaccination on antibody response and influenza-like illness among Australian healthcare workers after influenza vaccination in 2016.

Vaccine 2021 May 10. Epub 2021 May 10.

WHO Collaborating Centre for Reference and Research on Influenza, Royal Melbourne Hospital, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia; Fielding School of Public Health, University of California, Los Angeles, USA; Department of Infectious Diseases, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia. Electronic address:

Background: Epidemiological studies suggest that influenza vaccine effectiveness decreases with repeated administration. We examined antibody responses to influenza vaccination among healthcare workers (HCWs) by prior vaccination history and determined the incidence of influenza infection.

Methods: HCWs were vaccinated with the 2016 Southern Hemisphere quadrivalent influenza vaccine. Read More

View Article and Full-Text PDF

Changes in sialic acid binding associated with egg adaptation decrease live attenuated influenza virus replication in human nasal epithelial cell cultures.

Vaccine 2021 May 10. Epub 2021 May 10.

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, United States. Electronic address:

Live Attenuated Influenza Virus (LAIV) is administered to and replicates in the sinonasal epithelium. Candidate LAIV vaccine strains are selected based on their ability to replicate to a high titer in embryonated hen's eggs, a process that can lead to mutations which alter the receptor binding and antigenic structure of the hemagglutinin (HA) protein. In the 2012-2013 northern hemisphere vaccine, the H3N2 HA vaccine strain contained three amino acid changes - H156Q, G186V and S219Y - which altered HA antigenic structure and thus presumably decreased vaccine efficacy. Read More

View Article and Full-Text PDF

Poly(glycidyl methacrylate) macromolecular assemblies as biocompatible nanocarrier for the antimicrobial lysozyme.

Int J Pharm 2021 May 10:120695. Epub 2021 May 10.

Department of Chemical Engineering, Universidad de Alcalá, 28805 Alcalá de Henares, Madrid, Spain.

The antimicrobial lysozyme (Lys) was electrostatically incorporated to negatively charged crosslinked poly(glycidyl methacrylate) (c-PGMA) macromolecular assemblies. The resulting material was characterized by AFM, infrared spectra, water contact angle measurements and the staining with the primary amino specific dye fluorescamine. c-PGMA nanoparticles were successfully loaded with Lys reaching ratios of 27. Read More

View Article and Full-Text PDF

Two newly identified Haematococcus strains efficiently accumulated radioactive cesium over higher astaxanthin production.

Environ Res 2021 May 10:111301. Epub 2021 May 10.

Department of Biomedical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul, Republic of Korea; Department of Mechanical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul, Republic of Korea. Electronic address:

In this study, we investigated the morphological, genomic and bioaccumulation characteristics of two isolated Haematococcus strains (namely Goyang and Sogang), which were newly discovered in South Korea. Morphological analysis revealed that the isolated strains were unicellular and bi-flagellated green microalgae that formed thickened walls at the palmelloid or red-cyst phase. Phylogenetic analysis of 18S rRNA and rbcL gDNA sequences demonstrated that both strains were taxonomically related to the genus Haematococcus. Read More

View Article and Full-Text PDF

First report of Pythium aphanidermatum causing root rot of head lettuce in China.

Plant Dis 2021 May 13. Epub 2021 May 13.

Shandong Academy of Agricultural Sciences, Institute of Plant Protection, NO.202,Industrial north road,Licheng district, Jinan, Shandong, China, 250100;

Head lettuce (Lactuca sativa L.) is an important crop for fresh consumption in China. In Shandong Province, head lettuce is planted in spring and in autumn each year. Read More

View Article and Full-Text PDF

Will Mutations in the Spike Protein of SARS-CoV-2 Lead to the Failure of COVID-19 Vaccines?

J Korean Med Sci 2021 May 10;36(18):e124. Epub 2021 May 10.

Tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute for Tuberculosis Research, 8th Medical Center, Chinese PLA General Hospital, Beijing, China.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19), which has spread worldwide since it was first identified in Wuhan, China, at the end of 2019. With the global transmission of the virus, a large number of SARS-CoV-2 variants have also appeared, especially, emerging strains that have recently been discovered in the United Kingdom (variant 20I/501Y.V1, lineage B. Read More

View Article and Full-Text PDF

Effective removal of heavy metals from industrial effluent wastewater by a multi metal and drug resistant Pseudomonas aeruginosa strain RA-14 using integrated sequencing batch reactor.

Environ Res 2021 May 8:111240. Epub 2021 May 8.

Grassland and Forage Division, National Institute of Animal Science, RDA, Seonghwan-Eup, Cheonan-Si, Chungnam, 330-801, Republic of Korea. Electronic address:

Sequencing batch reactor (SBR) is useful in removal of both non-biodegradable and biodegradable contaminants from wastewater. The main aim of the present investigation was to evaluate the potential of biocatalyst strain RA-14 on heavy metal removal under SBR. The selected strain was screened from the soil sediment contaminated with heavy metals. Read More

View Article and Full-Text PDF

Overcoming the Challenges of Pyrazinamide Susceptibility Testing in Clinical isolates.

Antimicrob Agents Chemother 2021 May 10. Epub 2021 May 10.

Irish Mycobacteria Reference Laboratory, St. James's Centre for Laboratory Medicine and Molecular Pathology, St. James's Hospital, Dublin, Ireland.

Pyrazinamide (PZA) is one of the first-line agents used for the treatment of tuberculosis. However, current phenotypic PZA susceptibility testing in the BACTEC MGIT 960 system is unreliable and false resistance is well documented. Rapid identification of resistance-associated mutations can confirm the phenotypic result. Read More

View Article and Full-Text PDF

First Report of Bud Soft Rot on Agave angustifolia Caused by Pantoea dispersa in Mexico.

Plant Dis 2021 May 10. Epub 2021 May 10.

Facultad de Ciencias Agropecuarias y Ambientales de la Universidad Autónoma de Guerrero, Departamento de Agronomía, Iguala de la Independencia, C.P. 40020, Guerrero, Mexico;

The agave (Agave spp.) is an important crop in México, with 120,897 ha grown mainly for alcoholic beverage production (SIAP, 2019). In September 2020, in the municipality of Huitzuco de los Figueroa (18. Read More

View Article and Full-Text PDF

Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain.

Comput Struct Biotechnol J 2021 4;19:2806-2818. Epub 2021 May 4.

QUT, Centre for Genomics and Personalised Health, Cancer and Ageing Research Program, School of Chemistry and Physics, Faculty of Science and Engineering, Institute of Health and Biomedical Innovation, 2 George Street, Brisbane, QLD 4000, Australia.

SARS-CoV-2 has rapidly spread throughout the world's population since its initial discovery in 2019. The virus infects cells via a glycosylated spike protein located on its surface. The protein primarily binds to the angiotensin-converting enzyme-2 (ACE2) receptor, using glycosaminoglycans (GAGs) as co-receptors. Read More

View Article and Full-Text PDF

Comparing cytokine production and clinical response following vaccination with BCG Moreau and BCG Russia strains in a Brazilian infant population.

Vaccine 2021 May 5. Epub 2021 May 5.

Laboratório Avançado de Saúde Pública, Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (IGM/Fiocruz), Salvador, Bahia 40296 710, Brazil; Departamento de Ciências da Vida, Universidade Estadual da Bahia (UNEB), Salvador, BA 41150 000, Brazil. Electronic address:

Introduction: BCG is the only licensed vaccine against tuberculosis (TB) and, in Brazil, comprises part of the recommended vaccine schedule within the first month of life. Due to a local manufacturing shortage of BCG Moreau, BCG Russia was introduced in 2017 by the Brazilian Ministry of Health.

Objective: To evaluate differences in immune responses induced by BCG Moreau and BCG Russia in infants, in addition to scar formation. Read More

View Article and Full-Text PDF

p-Coumaric acid loaded into liquid crystalline systems as a novel strategy to the treatment of vulvovaginal candidiasis.

Int J Pharm 2021 May 5:120658. Epub 2021 May 5.

Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.

Vulvovaginal candidiasis (VVC) is an extremely common type of vaginal infection, which is mainly caused by Candida albicans. However, non-albicans Candida species are frequently more resistant to conventional antifungal agents and can represent up to 30% of cases. Due to side effects and increasing antifungal resistance presented by standard therapies, phenolic compounds, such as p-coumaric acid (p-CA), have been studied as molecules from natural sources with potential antifungal activity. Read More

View Article and Full-Text PDF

Susceptibility variation to the main pathogens of Crassostrea gigas at the larval, spat and juvenile stages using unselected and selected oysters to OsHV-1 and/or V. aestuarianus.

J Invertebr Pathol 2021 May 5:107601. Epub 2021 May 5.

IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia. F-34090 Montpellier, France.

French commercial hatcheries are massively producing Crassostrea gigas selected for their higher resistance to OsHV-1, and soon should also implement selection for increasing resistance to Vibrio aestuarianus. The first objective of this study was to optimize the breeding programs for dual resistance to OsHV-1 and V. aestuarianus to determine the earliest life stage for which oysters are able to develop disease resistance. Read More

View Article and Full-Text PDF

Use of an animal model of disease for toxicology enables identification of a juvenile no observed adverse effect level for cyclocreatine in creatine transporter deficiency.

Regul Toxicol Pharmacol 2021 May 4;123:104939. Epub 2021 May 4.

Lumos Pharma, 4200 Marathon Blvd Suite 200, Austin, TX, 78756, USA. Electronic address:

In standard general toxicology studies in two species to support clinical development, cyclocreatine, a creatine analog for the treatment of creatine transporter deficiency, caused deaths, convulsions, and/or multi-organ pathology. The potential translatability of these findings to patients was evaluated by comparing toxicity of cyclocreatine in wild-type mice to creatine transporter-deficient mice, a model of the human disease. A biodistribution study indicated greater accumulation of cyclocreatine in the brains of wild-type mice, consistent with its ability to be transported by the creatine transporter. Read More

View Article and Full-Text PDF

The impact of foot shock-induced stress on pain-related behavior associated with burn injury.

Burns 2021 Apr 20. Epub 2021 Apr 20.

Department of Anesthesiology, University of Kansas Medical Center, Kansas City, KS 66160, USA.

Acute pain is prevalent following burn injury and can often transition to chronic pain. Prolonged acute pain is an important risk factor for chronic pain and there is little preclinical research to address this problem. Using a mouse model of second-degree burn, we investigated whether pre-existing stress influences pain(sensitivity) after a burn injury. Read More

View Article and Full-Text PDF

Entry-competent-replication-abortive African horse sickness virus strains elicit robust immunity in ponies against all serotypes.

Vaccine 2021 May 3. Epub 2021 May 3.

Department of Infection Biology, Faculty of Infectious and Tropical Disease, London School of Hygiene and Tropical Medicine, United Kingdom. Electronic address:

African horse sickness virus (AHSV) is an Orbivirus within the Reoviridae family, spread by Culicoides species of midges, which infects equids with high mortality, particularly in horses and has a considerable impact on the equine industry. In order to control the disease, we previously described Entry Competent Replication Abortive (ECRA) virus strains for each of the nine distinct AHSV serotypes and demonstrated their potential as vaccines, first in type I interferon receptor (IFNAR-/-) knockout mice, and then in ponies. In this report we have investigated whether or not a combination ECRA vaccine comprising nine vaccine strains as two different cocktails is as efficient in ponies and the duration of the immunity triggered by ECRA vaccines. Read More

View Article and Full-Text PDF

Enhanced diversity and rock-weathering potential of bacterial communities inhabiting potash trachyte surface beneath mosses and lichens - A case study in Nanjing, China.

Sci Total Environ 2021 Apr 26;785:147357. Epub 2021 Apr 26.

Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.

Mosses and lichens have been shown to play an important role in enhancing global chemical weathering of the surface rock. However, there are no studies concerning the effects of mosses and lichens on the microbial communities inhabiting rock surfaces. In this study, culture-dependent and culture-independent analyses were employed to compare the diversity, composition, and rock-weathering activity of bacterial communities inhabiting potash trachyte surfaces covered by mosses (MR) and lichens (LR) with those inhabiting surrounding bare rock surfaces (BR). Read More

View Article and Full-Text PDF

Surface-enhanced Raman spectroscopy for the identification of tigecycline-resistant E. coli strains.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 24;258:119831. Epub 2021 Apr 24.

Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan.

Tigecycline (TGC) is recognised as last resort of drugs against several antibiotic-resistant bacteria. Bacterial resistance to tigecycline due to presence of plasmid-mediated mobile TGC resistance genes (tet X3/X4) has broken another defense line. Therefore, rapid and reproducible detection of tigecycline-resistant E. Read More

View Article and Full-Text PDF

Personalising left-ventricular biophysical models of the heart using parametric physics-informed neural networks.

Med Image Anal 2021 Apr 20;71:102066. Epub 2021 Apr 20.

Institute for Biomedical Engineering, ETH Zurich and University Zurich, Zurich, Switzerland.

We present a parametric physics-informed neural network for the simulation of personalised left-ventricular biomechanics. The neural network is constrained to the biophysical problem in two ways: (i) the network output is restricted to a subspace built from radial basis functions capturing characteristic deformations of left ventricles and (ii) the cost function used for training is the energy potential functional specifically tailored for hyperelastic, anisotropic, nearly-incompressible active materials. The radial bases are generated from the results of a nonlinear Finite Element model coupled with an anatomical shape model derived from high-resolution cardiac images. Read More

View Article and Full-Text PDF

Chemical Constitution and Antimicrobial Activity of Kefir Fermented Beverage.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.

Kefir beverage (KB) is a fermented milk initiated by kefir grains rich with starter probiotics. The KB produced in this study seemed to contain many chemical compounds elucidated by gas chromatography-mass spectrometry (GC-MS) and IR spectra. These compounds could be classified into different chemical groups such as alcohols, phenols, esters, fatty esters, unsaturated fatty esters, steroids, polyalkenes, heterocyclic compounds and aromatic aldehydes. Read More

View Article and Full-Text PDF

Roseomonas hellenica sp. nov., isolated from roots of wild-growing Alkanna tinctoria.

Syst Appl Microbiol 2021 Apr 17;44(3):126206. Epub 2021 Apr 17.

Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Fac. Sciences, Ghent University, Gent, Belgium.

Two Gram-negative, aerobic, rod-shaped and yellow-orange pigmented bacterial strains (LMG 31523 and LMG 31524) were isolated from roots of wild-growing Alkanna tinctoria plants collected near Thessaloniki, Greece. Analysis of their 16S rRNA gene sequences revealed that they form a separate cluster related to the genus Roseomonas. A comparative whole genome analysis of the two strains and the type strains of related Roseomonas species revealed average nucleotide identity values from 78. Read More

View Article and Full-Text PDF

Persistence of neutralizing antibody and its protective efficacy induced by a live attenuated tetravalent dengue vaccine, KD-382, in cynomolgus monkeys.

Vaccine 2021 May 1. Epub 2021 May 1.

KM Biologics Co., Ltd., 1-6-1 Okubo, Kita-ku, Kumamoto-shi, Kumamoto 860-8568, Japan. Electronic address:

An effective dengue vaccine should induce a long-lasting immune response against all four serotypes simultaneously with a minimum number of immunizations. Our live attenuated tetravalent dengue vaccine candidate, KD-382, was developed using a classical host range mutation strategy (no addition of artificial genetic modification). In our previous study, cynomolgus monkeys immunized with a single dose of KD-382 seroconverted to all four serotypes. Read More

View Article and Full-Text PDF

Synthesis, biological, and photophysical studies of molecular rotor-based fluorescent inhibitors of the trypanosome alternative oxidase.

Eur J Med Chem 2021 Apr 16;220:113470. Epub 2021 Apr 16.

Instituto de Química Médica, IQM-CSIC, Juan de la Cierva 3, E-28006, Madrid, Spain. Electronic address:

We have recently reported on the development and trypanocidal activity of a class of inhibitors of Trypanosome Alternative Oxidase (TAO) that are targeted to the mitochondrial matrix by coupling to lipophilic cations via C14 linkers to enable optimal interaction with the enzyme's active site. This strategy resulted in a much-enhanced anti-parasite effect, which we ascribed to the greater accumulation of the compound at the location of the target protein, i.e. Read More

View Article and Full-Text PDF

Synthesis of alkynylated 1,2,4-oxadiazole/1,2,3-1H-triazole glycoconjugates: Discovering new compounds for use in chemotherapy against lung carcinoma and Mycobacterium tuberculosis.

Eur J Med Chem 2021 Apr 18;220:113472. Epub 2021 Apr 18.

Department of Chemistry, Federal Rural University of Pernambuco, Dois Irmãos, 52171-900, Recife, Brazil. Electronic address:

A total of forty-three compounds were synthesized, including thirty-two new ones. Among those compounds, seventeen were selected and tested on human tumor cell lines: PC-3 (prostate adenocarcinoma), HCT-116 (colorectal tumor), NCIH-460 (lung carcinoma), SKMEL-103 (melanoma) and AGP-01 (gastric tumor). Alkynylated 1,2,4-oxadiazoles 2m, 3g and 3k exhibited antiproliferative activities against NCIH-460 in culture. Read More

View Article and Full-Text PDF

FT-IR and GC-MS analyses of potential bioactive compounds of cow urine and its antibacterial activity.

Saudi J Biol Sci 2021 Apr 1;28(4):2432-2437. Epub 2021 Feb 1.

Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar - 249404, Uttarakhand, India.

The main emphasis of this study was to identify the bioactive compounds responsible for antibacterial activity of cow urine isolated by thin layer chromatography. The most effective bioactive fraction was analysed by FT-IR and GC-MS analyses. Among the four major fractions (EW1, EW2, CA1 and CA2) obtained by TLC profiling, EW1 was found most active against bacterial strains viz. Read More

View Article and Full-Text PDF

Prophylactic and therapeutic vaccine against Pseudomonas aeruginosa keratitis using bacterial membrane vesicles.

Vaccine 2021 Apr 29. Epub 2021 Apr 29.

Department of Molecular Biodefense Research, Graduate School of Medicine, Yokohama City University, Yokohama 236-0004, Japan. Electronic address:

Purpose: Pseudomonas aeruginosa (P. aeruginosa) infection is one of the major causes of keratitis. However, effective prophylactic and therapeutic vaccines against P. Read More

View Article and Full-Text PDF

Femoral strength and strains in sideways fall: Validation of finite element models against bilateral strain measurements.

J Biomech 2021 Apr 17;122:110445. Epub 2021 Apr 17.

Department of Biomedical Engineering, Lund University, Sweden.

Low impact falls to the side are the main cause of hip fractures in elderly. Finite element (FE) models of the proximal femur may help in the assessment of patients at high risk for a hip fracture. However, extensive validation is essential before these models can be used in a clinical setting. Read More

View Article and Full-Text PDF